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Full-Text Articles in Veterinary Medicine
Genetic Characteristics Of Avian Influenza A Viruses Isolated From Thai Free-Grazing Ducks And Their Pathogenicity Potential In Animal Models, Supassama Chaiyawong
Genetic Characteristics Of Avian Influenza A Viruses Isolated From Thai Free-Grazing Ducks And Their Pathogenicity Potential In Animal Models, Supassama Chaiyawong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Influenza A viruses (IAVs) can infect several animal species and humans. In Thailand, free-grazing ducks are reservoir hosts of influenza A virus. The free-grazing ducks are potentially interface with wild birds and domestic birds and subsequently cause interspecies transmission of IAVs. This thesis composes of three study phases. Phase 1 is surveillance of influenza A in free-grazing ducks. Phase 2 is genetic characterization and diversity analysis of influenza A viruses from free-grazing ducks. Phase 3 is challenge of influenza A viruses isolated from free-grazing ducks in animal models. For phase 1, multi-years surveillance of IAVs in free-grazing ducks was conducted …
Detection And Study Of Genetic Diversity Of Selected Vector-Borne Protozoa And Rickettsia In Buffaloes In Thailand By Biomolecular Method, Hoang Lan Anh Nguyen
Detection And Study Of Genetic Diversity Of Selected Vector-Borne Protozoa And Rickettsia In Buffaloes In Thailand By Biomolecular Method, Hoang Lan Anh Nguyen
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study aimed to determine the occurrence, geographical distribution and genetic diversity of tick and mosquito-borne protozoa and rickettsia in buffaloes by the PCR – sequencing approach. A total of 456 blood samples were collected in provinces of Thailand including Mukdahan, Uthai Thani, Lampang, Amnat Charoen, Nong Bua Lamphu, Phatthalung, Surin and Chachoengsao. DNA was extracted from buffalo blood and screened for the presence of pathogens, followed by sequencing. The results indicated that the overall prevalence of rickettsia, protozoa, and malaria were 41%, 25.4%, and 12.9% respectively. Double and triple infection of buffaloes with pathogens were observed in 19.1% of …
Genetic Characterization Of Canine Parvovirus And Circovirus In Dogs In Vietnam, Nguyen Manh Tuong
Genetic Characterization Of Canine Parvovirus And Circovirus In Dogs In Vietnam, Nguyen Manh Tuong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Canine parvovirus (CPV-2) emerged and caused severe enteritis in dogs since the 1970s. The last variant, CPV-2c is considered as the high virulent strain and spread ubiquitous worldwide. This strain was early detected in Vietnam since 2002, but the whole genome is still fragmentary. In this study, we attempted to gain the whole genome of CPV-2c from the Vietnamese infected dogs as the first report of the full-length CPV-2c sequence originated from Asia. The Vietnamese CPV-2c exhibited the common mutations in both structural and nonstructural proteins and shared a typical evolutionary pattern with Asian strains. Besides, Vietnamese sequences presented some …
Effect Of Fish Antifreeze Protein Type Iii Supplementation To Semen Extender On Cryopreserved Dog Spermatozoa, Rudal Jetta Bayusalaksa
Effect Of Fish Antifreeze Protein Type Iii Supplementation To Semen Extender On Cryopreserved Dog Spermatozoa, Rudal Jetta Bayusalaksa
Chulalongkorn University Theses and Dissertations (Chula ETD)
Ice binding protein such as antifreeze protein (AFP) type III have been used as a cryoprotectant and proven to improve sperm survival in some species but dog. Addition of Equex STM paste to semen freezing medium provides beneficial effects on cryopreserved dog sperm. Hence, the objectives of this study were to (a) evaluate the effects of AFP (Experiment I) and (b) its combination with Equex STM paste on cryopreserved dog spermatozoa (Experiment II). The semen samples were pooled to allow for a sufficient number of sperm and to reduce an individual variation. Only sperm-rich fractions with total motility more than …
Establishment Of Encapsulated Human Dental Pulp Stem Cell-Derived Insulin Producing Cells (Hdpsc-Ipcs) For Translational Stem Cell Based Diabetes Therapy, Suryo Kuncorojakti
Establishment Of Encapsulated Human Dental Pulp Stem Cell-Derived Insulin Producing Cells (Hdpsc-Ipcs) For Translational Stem Cell Based Diabetes Therapy, Suryo Kuncorojakti
Chulalongkorn University Theses and Dissertations (Chula ETD)
Current approach for diabetes treatment remained several adverse events varied from gastrointestinal to life-threatening symptoms. Regenerative therapy regarding Edmonton protocol has been facing serious limitations involving protocol efficiency and safety. This led to the study for alternative insulin-producing cell (IPC) resource and transplantation platform. In this study, evaluation of encapsulated human dental pulp stem cell (hDPSC)-derived IPCs (hDPSC-IPCs) by alginate (ALG) and pluronic F127-coated alginate (ALGPA) was performed. The results showed that ALG and ALGPA preserved hDPSC viability and allowed glucose and insulin diffusion in and out. ALG- and ALGPA-encapsulated hDPSC-IPCs maintained viability for at least 336 hours and sustained …
Resistome Analysis Of Aeromonas Veronii Strains Isolated From Diseased Outbreak In Tilapia Farms In Thailand, Rungnapa Sakulworakan
Resistome Analysis Of Aeromonas Veronii Strains Isolated From Diseased Outbreak In Tilapia Farms In Thailand, Rungnapa Sakulworakan
Chulalongkorn University Theses and Dissertations (Chula ETD)
Aeromonas veronii outbreaks in tilapia farming caused relatively high mortality. Moreover, it was resistant to many kinds of antimicrobial used in Thailand aquaculture. According to no CLSI standard, the determination of antimicrobial efficacy has been limited phenotypically; the genomics study is required. This research aims to analyze the resistome of A. veronii isolated from diseased Tilapia in Chainat, Nong Khai and Uttaradit province, Thailand. Twelve isolates of A. veronii were identified base on gyrB sequencing then determined the MIC value to eight antimicrobials (AMP, AML, GEN, ENR, OXO, OXY, SXT, and FFC). According to MIC patterns, five representatives were performed …
Chondroprotective Efficacy Of Undenatured Collagen Type Ii On Canine Osteoarthritis Secondary To Medial Patellar Luxation, Susolos Kunsuwanachai
Chondroprotective Efficacy Of Undenatured Collagen Type Ii On Canine Osteoarthritis Secondary To Medial Patellar Luxation, Susolos Kunsuwanachai
Chulalongkorn University Theses and Dissertations (Chula ETD)
Osteoarthritis (OA) is found secondary from medial patellar luxation (MPL) which is one of the most common diseases in small-breed dogs. Managements of the disease are surgical correction or a medical treatment. In the patients who are unable to do the surgical correction, non-steroidal anti-inflammatory drugs (NSAIDs) remains the drug of choice for OA therapy. Nowadays nutraceuticals have become more popular since there are no side effects once given for a long period of time. The purpose of this study was to evaluate the clinical chondroprotective effects of undenatured collagen type-II (UCII) on OA secondary to canine medial patellar luxation. …
Comparison Of The Physical Capacity Using 6-Minute Walk Test And Questionnaire Before And After Resection Of Elongated Soft Palate By Bipolar Sealing Device In French Bulldogs, Tamolwan Anyamaneecharoen
Comparison Of The Physical Capacity Using 6-Minute Walk Test And Questionnaire Before And After Resection Of Elongated Soft Palate By Bipolar Sealing Device In French Bulldogs, Tamolwan Anyamaneecharoen
Chulalongkorn University Theses and Dissertations (Chula ETD)
Brachycephalic airway obstruction syndrome (BAOS) is an anatomical abnormality of upper airway commonly found in brachycephalic breeds leading to clinical signs and physical capacity impairment. The purposes of this study were to compare clinical signs and physical capacity in French bulldogs between before and after surgical correction using questionnaire and 6-minute walk test (6-MWT). Twenty-seven French bulldogs were enrolled in this study and divided into normal group (n=7) and BAOS group (n=20). History taking, clinical signs and questionnaire information were recorded and 6-MWT was performed in both groups. BAOS dogs had significantly higher severity of clinical signs from questionnaire with …
Akt Expression And Lactose Synthesis During Heat Exposure In Saanen Goat, Nungnuch Saipin
Akt Expression And Lactose Synthesis During Heat Exposure In Saanen Goat, Nungnuch Saipin
Chulalongkorn University Theses and Dissertations (Chula ETD)
In tropical countries, high ambient temperature (HTa) is a fundamental factor that cause a decline in the mammary gland function in dairy animals. Dairy animals fed under HTa decreased milk yield (MY) as well as milk compositions. The current study focuses on the effect of HTa to decrease milk synthesis from natural and in vitro HTa condition. The first experiment was performed to investigate the effect of HTa from the natural condition in early lactating Saanen goat, 2-4 weeks of postpartum (2 PP-4 PP). Five animals were investigated during the winter season as control ambient condition. Six animals as the …
The Left Ventricular Functions And Heart Rate Variability In Dogs With Pulmonic Valvular Stenosis, Ploypanut Trikhun
The Left Ventricular Functions And Heart Rate Variability In Dogs With Pulmonic Valvular Stenosis, Ploypanut Trikhun
Chulalongkorn University Theses and Dissertations (Chula ETD)
Pulmonic valvular stenosis (PS) is one of the most common congenital heart diseases in dogs that can lead to the cardiac maladaptation. Also, PS can affect the cardiac autonomic nervous system (ANS). However, clinical information regarding to systolic function and cardiac ANS alteration in PS dogs had not been fully elucidated. The objectives of this study were to evaluate cardiac electrical property, left ventricular (LV) function, and to assess cardiac ANS from heart rate variability (HRV) analysis in PS dogs compared with healthy dogs. The dogs in this study were divided into 2 groups, PS dogs (n=13) and healthy control …
Inhibitory Effect Of Canine Amniotic Membrane Against Common Bacterial Pathogens Associated With Ulcerative Keratitis, Rajit Lohajaroensub
Inhibitory Effect Of Canine Amniotic Membrane Against Common Bacterial Pathogens Associated With Ulcerative Keratitis, Rajit Lohajaroensub
Chulalongkorn University Theses and Dissertations (Chula ETD)
Infectious ulcerative keratitis is one of the significant ocular diseases in dogs leading to aggressive damage of the cornea and possibly loss of vision. Canine amniotic membrane (CAM) transplant revealed satisfactory result in canine complicated ulcers. However, antimicrobial activity of CAM has not yet been elucidated. This study is to assess antimicrobial activities of fresh CAM on bacteria associated with ulcerative keratitis and to investigate antimicrobial peptide genes expression. A total of twenty-three CAMs from healthy puppies were collected. Nineteen CAMs were tested for antimicrobial activity by modified disk diffusion test. Four CAMS were tested for mRNA expression of antimicrobial …
Salivary Proteomic Analysis For Detection And Monitoring Treatment Response In Canine Oral Tumors, Sekkarin Ploypetch
Salivary Proteomic Analysis For Detection And Monitoring Treatment Response In Canine Oral Tumors, Sekkarin Ploypetch
Chulalongkorn University Theses and Dissertations (Chula ETD)
Tumors of the oral cavity account for 8% of all canine neoplasia. Malignant oral mass such as melanoma, oral squamous cell carcinoma (OSCC) and fibrosarcoma covers approximately 59% of canine oral neoplasia. Among these, canine oral melanoma (COM) is the most common oral tumors in dogs, with approximately 30–40% of all oral tumors. With high recurrence and/or distant metastasis rate, biomarkers are required for early detection, diagnosis, prognosis, and monitoring treatment response. Since saliva directly contacts tumors and saliva collection is non-invasive, easily accessible and cost effective, salivary biomarkers are suitable to support the evaluation of oral diseases. This study …
The Effect Of Estrogen And Chronic Mild Stress On Gabaergic System And Anxiety- Like Behavior In Ovariectomized Rat, Sushawadee Tongta
The Effect Of Estrogen And Chronic Mild Stress On Gabaergic System And Anxiety- Like Behavior In Ovariectomized Rat, Sushawadee Tongta
Chulalongkorn University Theses and Dissertations (Chula ETD)
Stress in daily life and estrogen deprivation are known factors contributed to anxiety and depression. In animal models, anxiety-liked behaviors were demonstrated in ovariectomized or stress-exposed subjects. However, it was not clarified whether anxiety-liked behavior would be worsen when ovariectomized animals were exposed to mild stressors. The aims of this study were then designed to investigate (1) the effect of estrogen deprivation and chronic mild stresses (CMS) on anxiety- and depressive-liked behaviors and (2) the effect of estrogen receptor activation i.e. ERβ or GPR30 in estrogen deprivation exposed to CMS on anxiety-liked behavior. The alterations of GABAergic transmission in the …
Systems Biology Approach For The Establishment Of Veterinary Bone Tissue Engineering: The Proteomics Of Cbm-Mscs And Cdpscs Osteogenic Models, Sirirat Nantavisai
Systems Biology Approach For The Establishment Of Veterinary Bone Tissue Engineering: The Proteomics Of Cbm-Mscs And Cdpscs Osteogenic Models, Sirirat Nantavisai
Chulalongkorn University Theses and Dissertations (Chula ETD)
The utilization of canine mesenchymal stem cells (cMSCs) with multipotent capabilities has been regarded for possible therapy of incorrigible bone disease. Although various sources of cMSCs show similar characteristics, they are different in osteogenic potential due to their original cellular sources. This study was designed to globally explore and analyze the in vitro differentiation potential and behavior of canine bone-marrow derived mesenchymal stem cells (cBM-MSCs) and canine dental pulp stem cells (cDPSCs) toward osteogenic lineage. An in vitro osteogenic differentiation potential of the cells was preliminarily compared in terms of alkaline phosphatase activity assay and Von Kossa staining. Global study …
Application Of Needle-Free Injection Technology For Foot And Mouth Disease Vaccination Via Intradermal Route, Sirirat Wataradee
Application Of Needle-Free Injection Technology For Foot And Mouth Disease Vaccination Via Intradermal Route, Sirirat Wataradee
Chulalongkorn University Theses and Dissertations (Chula ETD)
Foot and mouth disease (FMD) is an endemic disease in Thailand and caused severe economic loses. In order to prevent and control disease, the vaccination program is routinely administered for 2-3 times a year via subcutaneous route (SC). Even though, the vaccination is performed regularly, the outbreaks have often reported. The outbreaks occur due to the failure of number of immunity animal in population or herd immunity. The limitation of vaccine distribution to all animal population and difficulty of cattle restraint are forcible several farmers to failing to vaccinate their animals. Based on previous studies, the intradermal route (ID) can …
Innovation Of Gonadotropin Releasing Hormone (Gnrh)-Modified Nanocarrier Delivered To Induce Testicular And Mammary Cell Apoptosis, Chatwalee Boonthum
Innovation Of Gonadotropin Releasing Hormone (Gnrh)-Modified Nanocarrier Delivered To Induce Testicular And Mammary Cell Apoptosis, Chatwalee Boonthum
Chulalongkorn University Theses and Dissertations (Chula ETD)
The main overall goal of this study is to investigate the application of modified chitosan as a potential vector for apoptotic gene delivery to gonadotropin-releasing hormone receptor (GnRHR)-expressing cells (i.e. testicular cells and mammary cancer cells). This study reported Gonadotropin Releasing Hormone-modified Chitosan (GnRH-CS) nanoparticle as a promising vector for targeted gene delivery, and a GnRH peptide was used for active targeting of a transgene in GnRHR expressing cells. The physiological characterizations of the prepared nanoparticle were investigated by proton nuclear magnetic resonance spectroscopy (1H NMR), Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and Nanosizer. The prepared GnRH-CS was …
Validation Of Lamp-Lfd Ehrlichia Canis Dna Sensor Kit, Pipat Jirapiti
Validation Of Lamp-Lfd Ehrlichia Canis Dna Sensor Kit, Pipat Jirapiti
Chulalongkorn University Theses and Dissertations (Chula ETD)
Diagnosis of Canine Monocytic Ehrlichiosis is in light of anamesis, visualized cytoplasmic morulae, clinical signs and laboratory test result of platelet counts. The PCR technique is always a definitive confirmatory test of Ehrlichia canis infection. However, the drawback of PCRs test is crucially needed of laboratory equipments. This drawback is somehow enabled newly DNA based technology to be developed in order to eliminate laborious thermocycler and gel electrophoresis. The LAMP-LFD is on focus by using a single temperature to amplify DNA and no gel electrophoresis involved to detect DNA product. LAMP-LFD is included in DNAsensor test kit that recently developed …
The Effect Of Monosodium Glutamate On Brain, Liver, Kidney, Testis, Ovary, Fertilization And Hatching Rate In Zebrafish (Danio Rerio), Chortip Sajjaviriya
The Effect Of Monosodium Glutamate On Brain, Liver, Kidney, Testis, Ovary, Fertilization And Hatching Rate In Zebrafish (Danio Rerio), Chortip Sajjaviriya
Chulalongkorn University Theses and Dissertations (Chula ETD)
Monosodium glutamate (MSG) is composed of sodium salt and amino acids that is commonly used to enhance food taste. Although it is safe at consumption level, the adverse effect of MSG had been reported. The present research was intended to evaluate the effect of MSG on the brain, liver, kidney, testes, and ovaries of zebrafish (Danio rerio) which were exposed from embryo stage to adult stage, along with the analyzing of the fertilization rate and hatching rate of zebrafish embryos. From the observation of histopathological changing of different organs of zebrafish after exposed to 10, 100, and 1000 ppm MSG …
Synbiotic Effects Of Jerusalem Artichoke (Helianthus Tuberosus) And Lactobacillus Rhamnosus-Supplemented Diet On Growth Performance, Immunological Parameters And Protection Against Aeromonas Veronii Challenge In Juvenile Red Tilapias (Oreochromis Spp.), Mariya Sewaka
Chulalongkorn University Theses and Dissertations (Chula ETD)
Synbiotics, a synergistic combination of probiotics and prebiotics, are now highlighted as one of the most practical nutritional supplements in red tilapia farms. The aim of the present study was to assess the effect of the dietary inclusion of Jerusalem artichoke, Lactobacillus rhamnosus, or their combination, on serum biochemical, growth performance, intestinal morphology, immune parameters, intestinal bacterial count and protection against Aeromonas veronii in juvenile red tilapia (Oreochromis spp.). One hundred and eighty male red tilapias (average body weight of 14.05±0.42 g) were distributed into six 1000-liter tanks containing water under continuous aeration and an average temperature of 25–28°C. Tilapias …
Efficacy Of Tetracycline And Enrofloxacin Against Wolbachia Spp. In Microfilaria Of Brugia Pahangi, Pimsiri Piromkij
Efficacy Of Tetracycline And Enrofloxacin Against Wolbachia Spp. In Microfilaria Of Brugia Pahangi, Pimsiri Piromkij
Chulalongkorn University Theses and Dissertations (Chula ETD)
Brugia pahangi is a major causative agent of animal lymphatic filariasis, one of the neclected tropical disease. Recent studies have also indicated that B. pahangi can cause clinical infection in human, with clinical appearances which are consistent with human lymphatic filariasis. Nowadays, the recognition of Wolbachia as new alternative drug choice for treatment and control of lymphatic filariasis is the most important in anti-filarial chemotherapy. Since, Wolbachia play important roles, including the development, viability, fertility and fecundity of filarial parasites. Depletion of Wolbachia results in sterilization, stunting and death of filarial worms. In this study, we investigated the effects of …
Efficacy And Safety Evaluations Of Topical Proretinal Nanoparticles, Benchaphorn Limcharoen
Efficacy And Safety Evaluations Of Topical Proretinal Nanoparticles, Benchaphorn Limcharoen
Chulalongkorn University Theses and Dissertations (Chula ETD)
Retinal (retinaldehyde) is one of natural vitamin A metabolites. It is widely used in clinical dermatology such as acne, acne scar, photoaging, wrinkles, psoriasis, skin neoplasia and seborrheic dermatitis. Retinal is a pivotal key to regulate keratinocyte proliferation and differentiation in the epidermis resulting in epidermal thickening and inhibit collagen destruction in the dermis. However, topical application of retinal is still irritative to the skin and chemically and photochemically unstable. Topically applied retinal can induce a retinoid dermatitis. This inflammation is induced by an overload of non-physiological amounts of exogenous retinoic acid in the skin. The adverse effects and physicochemical …
Genome Characterization Of Quinolone Resistance Associated Genes Of Flavobacterium Columnare Isolated From Columnaris Diseased Asian Sea Bass (Lates Calcarif)Er), Putita Chokmangmeepisarn
Genome Characterization Of Quinolone Resistance Associated Genes Of Flavobacterium Columnare Isolated From Columnaris Diseased Asian Sea Bass (Lates Calcarif)Er), Putita Chokmangmeepisarn
Chulalongkorn University Theses and Dissertations (Chula ETD)
Flavobacterium columnare is Gram negative bacteria which caused columnaris disease in various kinds of freshwater fishes. This study is the first report of F. columnare infection in freshwater culturing Asian sea bass in Thailand. Phenotypic characterization, antimicrobial susceptibility test and quinolone resistant-associated genes of F. columnare were performed. A total 15 of F. columnare were isolated from diseased fishes and phylogenetic analysis revealed that these isolates were belonged to genetic group 2 and 4. From disk diffusion test results, 5 and 6 isolates were resistant to oxolinic (OA) and nalidixic acid (NA) respectively. The highest MIC values to OA and …
Surveillance Of Influenza A Viruses In A Live Bird Market, Yangon, Myanmar During 2017-2018, Khin Thurain
Surveillance Of Influenza A Viruses In A Live Bird Market, Yangon, Myanmar During 2017-2018, Khin Thurain
Chulalongkorn University Theses and Dissertations (Chula ETD)
Live bird markets play a significant role in highly pathogenic avian influenza (HPAI) virus spread and transmission. In Myanmar, the first HPAI-H5N1 outbreak was reported in 2006. Since 2014, routine influenza A virus surveillance in LBMs have been conducted in Myanmar by the Livestock Breeding and Veterinary Department in cooperation with FAO. However, there is limited information on virus characterization and evolution of influenza viruses in Myanmar. The objectives of this thesis were to detect influenza virus subtypes circulating in different poultry species from Mingalar-Taung-Nyunt live bird market (MTN-LBM) and to determine genetic characteristics and genetic diversity of influenza A …
Appropriate Campylobacter Detection Method And Genetic Variation Of Campylobacter Jejuni In Broilers, Natnicha Phetsri
Appropriate Campylobacter Detection Method And Genetic Variation Of Campylobacter Jejuni In Broilers, Natnicha Phetsri
Chulalongkorn University Theses and Dissertations (Chula ETD)
Campylobacter has been known as an important cause of gastroenteritis in humans. The primary source is raw or undercooked poultry meat. Previous study showed that Campylobacter isolated from slaughterhouses were associated with Campylobacter isolated from broiler farms. Thus, detection of Campylobacter flock status and sorting flocks prior to slaughtering processes may help prevent Campylobacter contamination between Campylobacter-positive and negative flocks during slaughtering processes. Unfortunately, appropriate sample type and isolation method have not been clearly specified for Campylobacter detection at the farm level. Because Campylobacter are fastidious bacteria, improper detection method can attribute to false negative results. Therefore, the objectives of …
Development Of Reverse Transcription Loop-Mediated Isothermal Amplification Combine With Lateral Flow Device As A Detection Assay For Swine Influenza Virus, Albarriyati Ummi Asih
Development Of Reverse Transcription Loop-Mediated Isothermal Amplification Combine With Lateral Flow Device As A Detection Assay For Swine Influenza Virus, Albarriyati Ummi Asih
Chulalongkorn University Theses and Dissertations (Chula ETD)
Swine influenza causes high morbidity and low mortality in pigs. The disease spreads rapidly in pig farms causing the delay of pig weight to reach market. Moreover, pigs play important role as "mixing vessel" of influenza viruses. Pigs can support infection and replication of influenza viruses from avian, human and swine origins. These lead to major concerns of the important of swine influenza which are not only the economic impacts but also the human health. There is the need of accurate and rapid diagnosis for swine influenza virus (SIV) to minimize further spreading of the viruses in pig population and …
Pedv Monitoring Using Oral Fluid, Feces And Surface Swab Samples After The Ped Outbreak, Busayamas Jantrasakul
Pedv Monitoring Using Oral Fluid, Feces And Surface Swab Samples After The Ped Outbreak, Busayamas Jantrasakul
Chulalongkorn University Theses and Dissertations (Chula ETD)
A total of 220 pooled samples from 3 collection times were conducted. Eighty pooled samples from the first collection at 1 month after PED outbreak (1MAO) included 10 pooled oral fluid samples, 30 pooled fecal samples and 40 pooled surface swab samples. Eighty-five pooled samples from the second collection at 2MAO included 10 pooled oral fluid samples, 30 pooled fecal samples and 45 pooled surface swab samples. Fifty-five pooled samples from the third collection at 8MAO included 5 pooled oral fluid samples, 15 pooled fecal samples and 35 pooled surface swab samples. All samples collected from the PED-affected swine farm …
Phenotypic And Genotypic Characteristics Of Extended-Spectrum Β-Lactamase (Esbl) Production And Colistin-Resistance In Salmonella Enterica And Escherichia Coli Isolated From Pigs And Their Meat Products In The Border Provinces Between Thailand And Cambodia, Lao Pdr And Myanmar, Kyaw Phyoe Sunn
Chulalongkorn University Theses and Dissertations (Chula ETD)
A total of 809 samples of pig rectal swab from slaughterhouses (n=441) and pork from retail market (n=368) were collected in the border provinces among Thailand, Cambodia, Lao PDR and Myanmar between October 2016 and March 2017. The objective of this study was to determine resistance to extended-spectrum ß-lactams (ESBLs) and colistin in Salmonella enterica and Escherichia coli. A total of Salmonella (n=463) and E. coli (n=767) were collected and determined for the ESBL-production and for the Minimum Inhibitory Concentrations (MICs) of colistin and; the presence of ESBL gene and mcr gene. The results showed that the prevalence of Salmonella …
Genetic Characteristics Of Avian Influenza Viruses Isolated From Ducks In Java Island, Indonesia Year 2015-2017, Lestari -
Chulalongkorn University Theses and Dissertations (Chula ETD)
Avian influenza becomes public health concern over years due to the serious impacts in both animal and human health. This study aimed to identify and characterize avian influenza viruses isolated from ducks in Java Indonesia during 2015-2017. Total 50 samples of previously identified as avian influenza viruses were acquired from the virus/culture collections of Disease Investigation Center (DIC) Wates Yogyakarta, Indonesia. The samples were recovered from either the avian influenza surveillance or avian influenza outbreaks in ducks in 3 provinces, East Java (n=26), Central Java (n=16), and Yogyakarta (n=8). Then the samples were processed for influenza A virus screening by …
Genome Characterization Of Hahella Chejuensis Strain Hn01, A Pathogen Of Red Egg Disease In Tilapia (Oreochromis Sp.), Putu Cri Devischa Gallantiswara
Genome Characterization Of Hahella Chejuensis Strain Hn01, A Pathogen Of Red Egg Disease In Tilapia (Oreochromis Sp.), Putu Cri Devischa Gallantiswara
Chulalongkorn University Theses and Dissertations (Chula ETD)
Red eggs disease is an emerging disease occurred in Tilapia hatcheries which caused by a Gram-negative bacterium called Hahella chejuensis HN01. The disease infects to all the stage of fish eggs and reduces the hatchability. However, there are limited study about the bacterial pathogenicity. In this study, we analyzed the phylogenetic relationship and characterized the virulence traits of Hahella chejuensis by using genomic approach. The bacterial genome was sequenced using Illumina Miseq platform. The phylogenetic tree based on several housekeeping genes confirmed that the bacterium was belong to Genus Hahella and species chejuensis. the Average Nucleotide Identity calculation with the …
Detection Of Antimicrobial Resistance Determinants In Vibrio Parahaemolyticus Isolated From Cultivated Oysters And Estuarine Waters, Winn Khant
Chulalongkorn University Theses and Dissertations (Chula ETD)
In this study, we aimed to detect antimicrobial resistance determinants and virulence factor genes in V. parahaemolyticus isolates from cultivated oysters and estuarine waters in southern Thailand. A total of 594 V. parahaemolyticus isolates were collected from pooled oysters (n=361) and estuarine waters (n=233). The samples were collected monthly between March 2016 and February 2017 from Thap Put district, Phang Nga province in southern Thailand. Confirmation of V. parahaemolyticus was carried out by PCR assay detecting species-specific (tlh) gene and the presence of virulence genes (tdh and trh). Antimicrobial susceptibility test was performed in eight antimicrobials, and the occurrence of …