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Full-Text Articles in Veterinary Medicine
Detection And Distribution Of Enterotoxin And Adhesin Genes Of Staphylococcus Pseudintermedius Isolated From Dog, Human, And Environment, Nathita Phumthanakorn
Detection And Distribution Of Enterotoxin And Adhesin Genes Of Staphylococcus Pseudintermedius Isolated From Dog, Human, And Environment, Nathita Phumthanakorn
Chulalongkorn University Theses and Dissertations (Chula ETD)
To obtain the information of virulence factors associated with Staphylococcus pseudintermedius pathogenicity, host survival and adaptation, the study of staphylococcal enterotoxin (SE) genes and cell wall-associated (CWA) protein genes in S. pseudintermedius isolates from dogs, humans, and environment were performed. Human methicillin-resistant Staphylococcus pseudintermedius (MRSP) harbored the hightes number of SE genes at 12 out of 17 genes compared to those from dogs at 5 and environmental isolates at 3 gene types. For CWA protein genes or S. pseudintermedius surface proteins (sps), there was no an easy and fast method to detect them. Therefore, this study develop the multiplex PCR …
Dual Infection Between A Thai Isolate Highly Pathogenic Porcine Reproductive And Respiratory Syndrome Virus And Pandemic H1n1swine Influenza Virus In Weanling Pigs, Nattaphong Sangpratum
Dual Infection Between A Thai Isolate Highly Pathogenic Porcine Reproductive And Respiratory Syndrome Virus And Pandemic H1n1swine Influenza Virus In Weanling Pigs, Nattaphong Sangpratum
Chulalongkorn University Theses and Dissertations (Chula ETD)
After an introduction of pandemic H1N1 swine influenza virus (pdmH1N1 SIV) in the Thai swine farms, mild clinical outcome is observed and its role in porcine respiratory disease complex (PRDC) is questionable. A highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) has become endemic in the Thai swine farms. This study was conducted to determine the pathogenicity of the pdmH1N1 SIV when co-infected with a HP-PRRSV. Thirty-two weanling pigs from a free PRRSV, SIV, porcine circovirus type 2 (PCV-2) and Mycoplasma spp. commercial farm were randomly divided into 4 groups: Negative, PRRSV, SIV and Co-infection groups. Pigs in PRRSV …
Monitoring Of Antibiogram And Resistance Gene Profiles Among Escherichia Coli In Pig Production System, Kittitat Lugsomya
Monitoring Of Antibiogram And Resistance Gene Profiles Among Escherichia Coli In Pig Production System, Kittitat Lugsomya
Chulalongkorn University Theses and Dissertations (Chula ETD)
The antimicrobial resistant (AMR) bacteria in pig farms have been believed as an important source in food chain with public health concern. While some studies suggested transmission of AMR from pigs to humans may occur, but there was still needing to combine high resolution genomic data analysis with systematically collected epidemiological evidence to reconstruct patterns of AMR transmission between pigs and humans. The objectives of this study were to determine the occurrence and characterization the AMR phenotypes against 18 antimicrobials in pig producing system in both cross-sectional from fattening and longitudinal studies from newborn to slaughtering pigs and to evaluate …
Identification Of Porcine Circovirus Type 3 (Pcv3) In Thailand And Its Associated Syndrome, Roongtham Kedkovid
Identification Of Porcine Circovirus Type 3 (Pcv3) In Thailand And Its Associated Syndrome, Roongtham Kedkovid
Chulalongkorn University Theses and Dissertations (Chula ETD)
Porcine circovirus type 3 (PCV3) is a recently identified swine virus with currently unknown pathogenesis. To date, PCV3 has been found in many countries, indicating the wide spread of the virus. Therefore, there is an urgent need to clarify the detrimental role of PCV3. To better understand how PCV3 might influence the pig industry, various perspectives in swine production cycle should be considered. In this study, firstly, PCV3 was identified for the first time in Thailand. The study also showed that PCV3 has existed in the Thai pig population as early as 2005, suggesting that the virus might have long …
Immunophenotype, Molecular Diagnosis, Minimal Residual Disease, And Multidrug Resistance Protein Expression In Modified Cop, Modified Chop And Rescue Protocols Of Treated Canine Lymphomas, Sirintra Sirivisoot
Immunophenotype, Molecular Diagnosis, Minimal Residual Disease, And Multidrug Resistance Protein Expression In Modified Cop, Modified Chop And Rescue Protocols Of Treated Canine Lymphomas, Sirintra Sirivisoot
Chulalongkorn University Theses and Dissertations (Chula ETD)
Multicentric lymphoma is the most common hematopoietic tumors in dogs. Both B- or T-lymphocyte clones can originate to lymphoma. A gold standard method to differentiate each lymphocyte lineage is immunohistochemistry (IHC) by detecting specific proteins of B and T cell. A protein marker for T and B cells is CD3 and CD79a, respectively. However, another pan pre B-cell marker is Pax5 or B-cell specific activator protein. It is more specific and sensitive than CD79a because it expresses only in B-cell lymphoma cases. The first purpose of this research was to 1) determine Pax5 expression in canine lymphomas by IHC and …
Oral Fluid Samples Used For Prrs Diagnosis And Management, Yonlayong Woonwong
Oral Fluid Samples Used For Prrs Diagnosis And Management, Yonlayong Woonwong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Porcine reproductive and respiratory syndrome (PRRS) is an economically significant swine disease having a negative impact to the swine industry worldwide. Appropriate management strategies and diagnostic tests are crucial to successfully control PRRS virus (PRRSV). However, the application for oral fluid-based diagnosis has not been evaluated in the Thai farms previously. In this study, pre-extraction methods of oral fluids were evaluated to improve the PCR product yielded. Moreover, the oral fluids utilization for PRRSV monitoring during the gilt acclimatization and farrowing to nursery period was investigated. The results demonstrated that increasing sample volume might be a suitable simple method for …
Quantitative Microbial Risk Assessment Of Streptococcus Suis Serotype 2 From Pork Consumption, Phrutsamon Wongnak
Quantitative Microbial Risk Assessment Of Streptococcus Suis Serotype 2 From Pork Consumption, Phrutsamon Wongnak
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study aimed at determine prevalence and concentration of Streptococcus suis contamination in pork production chains as well as evaluate the health risk attributable to S. suis serotype 2 from pork consumption in Thailand. In total, 492 pig-to-pork and 480 environmental samples were collected from 4 pork production chains in Bangkok. Besides, total 1,036 pork samples were also collected from traditional and modern trades in Chiangmai, Phayao, Nan, Khonkaen, Mukdahan, Saraburi, Nakhonpathom, and Phang-nga provinces. All samples were enumerated using plate count method. S. suis isolates were confirmed by detecting cps2-j gene, followed by Quellung reaction. A stochastic risk assessment …
Quantitative Microbial Risk Assessment Of Klebsiella Pneumoniae To Ciprofloxacin From Pork In Bangkok, Rodjana Namkratok
Quantitative Microbial Risk Assessment Of Klebsiella Pneumoniae To Ciprofloxacin From Pork In Bangkok, Rodjana Namkratok
Chulalongkorn University Theses and Dissertations (Chula ETD)
Risk estimates are evaluated by using quantitative microbial risk assessment (QMRA) which is a scientific tool. The methodology can be applied to model of human adverse health effect associated with K. pneumoniae and ciprofloxacin-resistant K. pneumoniae (CRKP) from pork consumption in Bangkok. This study had four phases which were 1) sample collection, 2) bacterial isolation and enumeration, 3) antimicrobial susceptibility test and 4) risk assessment. A total of 378 pork samples from fresh markets were collected from six Bangkok areas which contained Central Bangkok, Eastern Bangkok, Northern Bangkok, Southern Bangkok, Upper Thonburi and Lower Thonburi. The mean prevalence of K. …
Application Of Autologous Blood-Derived Platelet-Rich Fibrin For Periodontitis Treatment In Dogs, Chatvadee Kornsuthisopon
Application Of Autologous Blood-Derived Platelet-Rich Fibrin For Periodontitis Treatment In Dogs, Chatvadee Kornsuthisopon
Chulalongkorn University Theses and Dissertations (Chula ETD)
Periodontitis is one of the most prevalent inflammatory oral diseases in dog. Non-surgical managements are conventionally performed to obtain healthy oral status. With progressive periodontitis, advanced periodontal surgery and also dental extraction become warranted with major disadvantages of trauma, bleeding, prolonged anesthetic time and hospitalization, and wound healing impairment. Hence, use of platelet-rich fibrin has been studied and considered one of the novel approaches to augment in periodontitis management. This study aimed to evaluate the efficacy of autologous blood-derived platelet-rich fibrin in an aspect of periodontitis treatment in a canine models. In this study, the experiment was done at maxillary …
The Effect Of Dexmedetomidine In Combination With Pethidine On Isoflurane Minimum Alveolar Concentration For Canine Antinociception, Kanjana Vinyunantakul
The Effect Of Dexmedetomidine In Combination With Pethidine On Isoflurane Minimum Alveolar Concentration For Canine Antinociception, Kanjana Vinyunantakul
Chulalongkorn University Theses and Dissertations (Chula ETD)
The effects of dexmedetomidine combined with pethidine on the minimum alveolar concentration (MAC) of isoflurane and cardiorespiratory variables in dogs were evaluated in thirty client-owned, healthy adult male dogs scheduled for castration at the Small Animal Teaching Hospital, Faculty of Veterinary Science, Chulalongkorn University. The dogs were allocated into Group 1 (n=6) receiving sterilized water for injections (placebo) as a negative control group, Group 2 (n=12) receiving 5 µg kg-1 of dexmedetomidine IM as a positive control group, and, Group 3 (n=12) receiving 5 µg kg-1 of dexmedetomidine and 5 mg kg-1 of pethidine IM. At 15 minutes after injection …
Computed Tomography Appearance Of Intra-Thoracic Lymph Nodes In Normal Cats, Ninlawan Thammasiri
Computed Tomography Appearance Of Intra-Thoracic Lymph Nodes In Normal Cats, Ninlawan Thammasiri
Chulalongkorn University Theses and Dissertations (Chula ETD)
The study of the appearance of intra-thoracic lymph nodes in the cat observed on the computed tomography which divided into 3 parts as following; the feline soft cadaver, clinically normal cats and the retrospective study of the feline patients. The result showed that the computed tomographic images of feline soft tissue cadavers could not detected any intra-thoracic lymph nodes. However, based on the study on 30 clinically normal cats which were mixed breed, age ranging from 4 months to 11 years old, body weight between 1.5 to 5.7 kilogram, 12 male cats (6 castrated) and 18 female cats (10 spayed) …
Intra-Articular Characteristics Of Dogs With Medial Patellar Luxation, Sethapong Jahrupatrakorn
Intra-Articular Characteristics Of Dogs With Medial Patellar Luxation, Sethapong Jahrupatrakorn
Chulalongkorn University Theses and Dissertations (Chula ETD)
The study investigated the relationship of degree of patellar luxation of dogs with joint radiography, intra-articular degenerative change of the stifle with medial patellar luxation (MPL), histopathologic change, and biomarker index. Thirty-eight stifles were divided into four groups: normal stifle joints (4), grades 2 (13), 3 (12), and 4 (9) MPL. Demographic data, lameness score, and degree of patellar luxation of all dogs were assessed and recorded. Radiographs were taken to assess osteophyte formation. Synovial tissue was collected for microscopic evaluation differential cell counts, and cytokines measurement. Stifle joints with MPL were scored during surgical correction. All observed data were …
Comparative Surgical Treatment Of Elongated Soft Palate In Brachycephalic Dogs Using The Ultrasonic Scalpel And The Conventional Incisional Techniques, Sunisa Thunyodom
Comparative Surgical Treatment Of Elongated Soft Palate In Brachycephalic Dogs Using The Ultrasonic Scalpel And The Conventional Incisional Techniques, Sunisa Thunyodom
Chulalongkorn University Theses and Dissertations (Chula ETD)
Brachycephalic airway obstruction syndrome (BAOS) is life threatening condition in brachycephalic dogs. Surgical correction of primary disorders including elongated soft palate and stenotic nares is indicated in all cases. Aim of this study was to compare the use of the ultrasonic scalpel with the conventional incision technique for staphylectomy. Twenty dogs with BAOS enrolled in this study were randomly assigned into conventional (N=10) or ultrasonic group (N=10). Respiratory score was evaluated and recorded prior to surgery. Staphylectomy time and bleeding were recorded. Respiratory, postoperative complication and pain scores were evaluated and recorded at days 1, 3, 7, 14 and 28 …
การประเมินทางพันธุกรรมของลักษณะการอยู่รอดของลูกสุกรที่สัมพันธ์กับขนาดครอกและน้ำหนักแรกเกิดรายตัว, ทิตยา วรวัฒนธรรม
การประเมินทางพันธุกรรมของลักษณะการอยู่รอดของลูกสุกรที่สัมพันธ์กับขนาดครอกและน้ำหนักแรกเกิดรายตัว, ทิตยา วรวัฒนธรรม
Chulalongkorn University Theses and Dissertations (Chula ETD)
ประเมินค่าพารามิเตอร์ทางพันธุกรรมของลักษณะการอยู่รอดของลูกสุกรแรกเกิด (PSB) และการอยู่รอดของลูกสุกรก่อนหย่านม (PSW) ร่วมกับลักษณะน้ำหนักแรกเกิดรายตัว (IBW) และลักษณะขนาดครอก (LS) จากข้อมูลการอยู่รอดของลูกสุกรจำนวน 14,389 บันทึก (1,413 ครอก) จากแม่สุกร 705 ตัว ข้อมูลการอยู่รอดของลูกสุกรพันธุ์แลนด์เรซ ยอร์กเชียร์ และดูร็อก บันทึกในฟาร์มที่เลี้ยงสุกรในโรงเรือนเปิดที่ไม่มีระบบระบายความร้อน เป็นข้อมูลของลูกสุกรที่เกิดระหว่างปี พ.ศ. 2549 ถึง 2560 ประมาณค่าองค์ประกอบความแปรปรวนด้วยวิธี Gibbs sampling สำหรับลักษณะแบบไบนารี่และวิธี Average Information Restricted Maximum Likelihood (AIREML) สำหรับลักษณะแบบต่อเนื่อง ผลการศึกษาพบว่า ลำดับท้อง ระยะเวลาการอุ้มท้องของแม่สุกร และกลุ่มของโรงเรือน-ปี-เดือนที่ลูกสุกรเกิด มีอิทธิพลต่อ PSB, PSW และ IBW อย่างมีนัยสำคัญทางสถิติ (p<0.05) นอกจากนี้ PSW และ IBW ยังได้รับอิทธิพลจากพันธุ์ และเพศของสุกร (p<0.05) สำหรับ LS พบว่าขึ้นอยู่กับอิทธิพลของพันธุ์ของลูกสุกร และกลุ่มของโรงเรือน-ปี-เดือนที่ลูกสุกรเกิด (p<0.05) ค่าอัตราพันธุกรรมของ PSB, PSW, IBW และ LS มีค่าเท่ากับ 0.23 ± 0.02, 0.36 ± 0.03, 0.38 ± 0.03 และ 0.22 ± 0.01 ตามลำดับ ค่าสหสัมพันธ์ทางพันธุกรรมระหว่าง IBW กับ PSB และ PSW มีค่าสูงเท่ากับ 0.77 ± 0.05 และ 0.85 ± 0.05 ตามลำดับ ในขณะที่ค่าสหสัมพันธ์ทางพันธุกรรมระหว่าง PSB กับ PSW มีค่าปานกลางเท่ากับ 0.69 ± 0.14 สำหรับลักษณะรายครอกพบว่าค่าสหสัมพันธ์ทางพันธุกรรมระหว่าง LS กับลักษณะเปอร์เซ็นต์การอยู่รอดของลูกสุกรแรกเกิด (%PSB) เปอร์เซ็นต์การอยู่รอดของลูกสุกรก่อนหย่านม (%PSW) และน้ำหนักแรกเกิดเฉลี่ย (ABW) มีค่าเป็นลบเท่ากับ -0.16 ± 0.03 -0.64 ± 0.02 และ -0.99 ± 0.001 ตามลำดับ จากค่าสหสัมพันธ์ทางพันธุกรรมแสดงให้เห็นว่า การคัดเลือกเพื่อเพิ่มน้ำหนักแรกเกิดของลูกสุกรอาจมีผลทำให้ PSB และ PSW เพิ่มขึ้น แต่หากคัดเลือกเพื่อเพิ่ม LS จะมีผลทำให้ %PSB, %PSW และ ABW ลดลง