Open Access. Powered by Scholars. Published by Universities.®
Articles 241 - 270 of 284
Full-Text Articles in Veterinary Medicine
Biomechanical And Biological Properties Of Cryopreserved Canine Amniotic Membrane, Nathawan Withavatpongtorn
Biomechanical And Biological Properties Of Cryopreserved Canine Amniotic Membrane, Nathawan Withavatpongtorn
Chulalongkorn University Theses and Dissertations (Chula ETD)
Amniotic membrane (AM) is the innermost fetal membrane. Due to its many known properties, human AM is a favorable corneal graft. Cryopreservation at -80ºC maintains most of biological properties. Due to shortage of supply, AM of other species are studied. The objective of this study is to explore the biomechanical and biological properties of canine AM (CAM) after cryopreservation for 7 and 30 days. CAMs were collected from healthy bitches undergoing cesarean sections. After preservation, they were tested for tensile strength by Universal Testing Machine, transparency by spectrophotometer, sterility by microbial culture, cell viability by trypan blue staining, and histological …
The Efficiency Of Pcso-524 On Feline Osteoarthritis Associated With Chronic Kidney Disease, Pemika Dulyapraphant
The Efficiency Of Pcso-524 On Feline Osteoarthritis Associated With Chronic Kidney Disease, Pemika Dulyapraphant
Chulalongkorn University Theses and Dissertations (Chula ETD)
Twenty-six osteoarthritic cats concurrent with chronic kidney disease (CKD) enrolled in this study. All cats were divided randomly into 2 groups; control group (n=12) received placebo and treatment group (n=14) received PCSO-524 for 60 days. Cats had verified stable CKD stage II and had treated and followed up for CKD more than 3 months were included in our study. Before starting the study (day 0), all cats were taken the coxofemoral joint radiographic imaging. Only cats with the radiographic osteoarthritic lesion(s) were recruited. All of them had blood profile check-up, urinalysis, blood pressure measurement, lameness score assessment, acute and chronic …
Chondroprotective Efficacy Of Pcso-524 On Canine Osteoarthritis Secondary To Medial Patellar Luxation, Ratthanan Sathienbumrungkit
Chondroprotective Efficacy Of Pcso-524 On Canine Osteoarthritis Secondary To Medial Patellar Luxation, Ratthanan Sathienbumrungkit
Chulalongkorn University Theses and Dissertations (Chula ETD)
Fifty stifles from Pomeranians and Chihuahuas with over five years old were enrolled in this study, which are 8 normal stifles and 42 medial patellar luxation (MPL) stifles. All MPL stifles were assigned randomly into 2 groups, including control and treatment groups. The control group was received placebo daily for 16 weeks, while treatment group was fed with PCSO-524 for 16 weeks. Lameness score, ultrasonographic findings, radiographic OA score and owner questionnaire (cBPI) were assessed at pre-treatment (D0) and post-treatment at week 2, 4, 8, 12 and 16. The ultrasonographic evaluations consisted of synovial fluid, articular cartilage, bone surface and …
Comparative Clinical Outcomes Of Elongated Soft Palate In Brachycephalic Dogs By Surgical Treatment Using The Ultrasonic Scalpel And Bipolar Sealing Device Techniques, Varit Wijitratanagorn
Comparative Clinical Outcomes Of Elongated Soft Palate In Brachycephalic Dogs By Surgical Treatment Using The Ultrasonic Scalpel And Bipolar Sealing Device Techniques, Varit Wijitratanagorn
Chulalongkorn University Theses and Dissertations (Chula ETD)
Brachycephalic airway obstruction syndromes (BAOS) are the most common respiratory problems in brachycephalic dogs. Surgical treatment which are staphylectomy and alaplasty is commonly used for correction primary lesions of BAOS. The purpose of this study is to compare clinical outcome of post staphylectomy using ultrasonic scalpel and bipolar sealing devices. Twenty brachycephalic dogs were included for staphylectomy and randomly separated into two groups. Surgical time, perioperative blood loss, pain score and clinical score of respiratory function were recorded at day 1 7 14 21 and 28 post-operative. The excised soft palate at day 0 and 21 was recorded lateral thermal …
Physiological And Immunological Responses Of Porcine Primary Endometrial Cells To Porcine Reproductive And Respiratory Syndrome (Prrs) Virus Infection, Muttarin Lothong
Physiological And Immunological Responses Of Porcine Primary Endometrial Cells To Porcine Reproductive And Respiratory Syndrome (Prrs) Virus Infection, Muttarin Lothong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Porcine reproductive and respiratory syndrome virus (PRRSV) is highly limited to only cell subsets that express PRRSV receptors. Reproductive organ revealing the typical signs of PRRSV infection may be the critical site of problem syndromes. Persistent PRRSV producing re-infection via horizontal or vertical transmission could not be eradicated from herds. This research examined the possibility of porcine endometrium to be a PRRSV permissive cell and serves as the primary cause of the persistent PRRSV. Cellular and immunological in response to PRRSV relevant to viral replication, shedding and re-circulation was assessed. The different outcomes between the different genotypes (type I vs. …
ผลของการเสริมกากชาต่อสมรรถภาพการผลิต การย่อยได้ปรากฏ และ การผลิตก๊าซมีเทนของแพะนมลูกผสม, รพีพัฒน์ สันโดษ
ผลของการเสริมกากชาต่อสมรรถภาพการผลิต การย่อยได้ปรากฏ และ การผลิตก๊าซมีเทนของแพะนมลูกผสม, รพีพัฒน์ สันโดษ
Chulalongkorn University Theses and Dissertations (Chula ETD)
งานวิจัยนี้มีวัตถุประสงค์เพื่อศึกษาผลของการใช้กากชา ต่อประสิทธิภาพการใช้อาหาร การย่อยได้ปรากฏ การเปลี่ยนแปลงของเมทาโบไลท์ในเลือด และการผลิตก๊าซมีเทน ในอาหารที่ใช้เลี้ยงแพะนมลูกผสม วางแผนการวิจัยแบบสลับ (3 x 3 crossover design) โดยใช้แพะนมลูกผสมพันธุ์ซาแนนจำนวน 9 ตัว น้ำหนักเฉลี่ย 30 ± 3.50 กิโลกรัม เป็นแม่แพะที่ผ่านการคลอดมาแล้ว (multiparous) และอยู่ในช่วงให้ผลผลิตน้ำนมหลังคลอด (lactation) การทดลองแบ่งออกเป็น 2 ช่วง โดยในช่วง 14 วันแรกจะเป็นช่วงของการปรับตัว ช่วงที่เหลืออีก 7 วันจะเป็นช่วงของการเก็บตัวอย่างและข้อมูล แพะแต่ละตัวได้รับอาหารทั้งสามสูตรที่มีการเสริมกากชาลงในสัดส่วนที่ 0 5 และ 10 เปอร์เซ็นต์น้ำหนักแห้งในรูปของอาหารผสมเสร็จ (Total mixed ration; TMR) (กลุ่มที่ 1 2 และ 3 ตามลำดับ) มีการคำนวณระดับโปรตีนและพลังงานเท่ากันทุกสูตร (isonitrogenous and isocaloric diets) ทำการเก็บตัวอย่าง ได้แก่ อาหาร เลือด ปัสสาวะ มูล น้ำนม และของเหลวจากกระเพาะรูเมน เพื่อที่จะประเมินค่าพารามิเตอร์ที่สังเกตหลายชนิด ผลการศึกษาพบว่า ไม่มีความแตกต่างกันทางสถิติในกลุ่มทดลองของปริมาณการกินได้ ผลผลิตน้ำนม ค่าเมทาโบไลท์ในเลือด (กลูโคส ไตรกลีเซอไรด์ โปรตีนรวม) ค่าพีเอชในกระเพาะรูเมน กรดไขมันระเหย (volatile fatty acids; VFAs) ค่าอัลลานโทอิน (allantoin) ค่าสมดุลไนโตรเจนและผลผลิตก๊าซมีเทน ส่วนประกอบของโปรตีนในน้ำนมในกลุ่มที่ 3 มีความแตกต่างกันทางสถิติ (p<0.05) เมื่อเปรียบเทียบกับกลุ่มที่ 1 และ 2 ค่าการย่อยได้ของวัตถุแห้งในกลุ่มที่ 3 สูงกว่าในกลุ่มที่ 2 อย่างมีนัยสำคัญทางสถิติ (p<0.05) แต่ไม่แตกต่างจากกลุ่มที่ 1 ส่วนกลุ่มที่ 2 มีค่าการย่อยได้ของสารอินทรีย์ โปรตีนและ NDF ต่ำที่สุดในทุกกลุ่ม อย่างไรก็ตาม กลุ่มที่ 3 มีค่าการย่อยได้ของ NDF และ ADF สูงที่สุดเมื่อเปรียบเทียบกับกลุ่มอื่น จากผลการศึกษาสรุปได้ว่า สามารถใช้กากชาได้ถึงระดับ 10 เปอร์เซ็นต์น้ำหนักแห้งในสูตรอาหาร เพื่อเป็นแหล่งโปรตีนในอาหารที่ใช้เลี้ยงแพะนมลูกผสมผสมซาเนนได้ โดยไม่ก่อให้เกิดผลเสียต่อปริมาณการให้ผลผลิตและนิเวศวิทยาในกระเพาะรูเมน อย่างไรก็ตาม การเสริมกากชาที่ระดับ 5 และ 10 เปอร์เซ็นต์น้ำหนักแห้งในสูตรอาหารไม่ส่งผลต่อการผลิตก๊าซมีเทน
Follicular Responsiveness To Equine Chorionic Gonadotropin In Cat Species: Study In In Vitro Culture, Ajjima Chansaenroj
Follicular Responsiveness To Equine Chorionic Gonadotropin In Cat Species: Study In In Vitro Culture, Ajjima Chansaenroj
Chulalongkorn University Theses and Dissertations (Chula ETD)
Equine chorionic gonadotropin (eCG) has been commonly used to induce estrus in several felid species. However, the mechanisms by which this gonadotropin regulates cat folliculogenesis are still unclear. In this present study, we investigated 1) the in vitro responsiveness of cat ovarian follicles at different follicle developmental stages to various eCG concentration; 2) tiger antral follicle responsiveness to eCG; 3) the influence of eCG combination with insulin-like growth factor I (IGF-I) and/or stem cell factor (SCF) on cat ovarian follicles at different developmental stages. Study 1, the isolated follicles from the ovaries of 22 cats were classified into three developmental …
The Development Of Elisa Kit To Detect Antibody Response Against Porcine Epidemic Diarrhea Virus From Colostrum And Milk, Anchalee Srijangwad
The Development Of Elisa Kit To Detect Antibody Response Against Porcine Epidemic Diarrhea Virus From Colostrum And Milk, Anchalee Srijangwad
Chulalongkorn University Theses and Dissertations (Chula ETD)
Porcine epidemic diarrhea is caused by porcine epidemic diarrhea virus (PEDV), which causes swine watery diarrhea and higher mortality rates in piglets less than one week of age. Outbreaks of PED affect to economic loss, especially in a swine industrial country. Many strategies were used for preventing the outbreak and infection of PEDV, especially maternal antibody stimulation in pigs and then transfer to piglet through colostrum or milk. Thus, the investigation of antibody level for the situation on the farm is required. The method which convenient and rapid than viral neutralization (VN) test is an enzyme-linked immunosorbent assay (ELISA). The …
Microfluidics-Based Single Cell Isolation And Trapping Of Canine Cutaneous Mast Cell Tumor Cells, Dettachai Ketpun
Microfluidics-Based Single Cell Isolation And Trapping Of Canine Cutaneous Mast Cell Tumor Cells, Dettachai Ketpun
Chulalongkorn University Theses and Dissertations (Chula ETD)
In this research, the primary objective was to develop a microfluidic platform utilized for the size-based single cell sorting of canine cutaneous mast cell tumor (MCT) cells which were categorized into 4 groups; I, II, III and IV, upon their individual sizes ranged from 5-9, 10-14, 15-19, and equal to or over than 20 mm respectively. And the secondary aim was to establish the microfluidic-based entrapping microdevice which had a capacity to ensnare single MCT cells into its triangular microwell array at the ratio of one cell per one microwell. The study consequence consistently indicated the accomplishment of device developments. …
Involucrin Expression In Three-Dimentional Cell Culture And Prognostic Parameters Of Canine Cutaneous Squamous Cell Carcinoma (Scc) In Canine Patients And In Ultraviolet Radiation Induced Cultured Cells, Nongnut Assawawongkasem
Involucrin Expression In Three-Dimentional Cell Culture And Prognostic Parameters Of Canine Cutaneous Squamous Cell Carcinoma (Scc) In Canine Patients And In Ultraviolet Radiation Induced Cultured Cells, Nongnut Assawawongkasem
Chulalongkorn University Theses and Dissertations (Chula ETD)
The objectives of this study are 1) to demonstrate the involucrin (INV) expression and prognostic parameters in normal skin and cutaneous squamous cell carcinoma (CSCC), 2) to develop the artificial epidermis in dog and 3) to study the effect of INV expression on pathogenesis of ultraviolet radiation induced CSCC in dog.The first experiment is to determine the protein expression by Immunofluorescence technic (IFC) and mRNA expression of INV,Cytokeratin 10 (CK10), Ki67 and p53 normal skin(n=6) and CSCC (n=6) in dog.The protein expression pattern was demonstrated that INV and CK10 were positive in intra-cytoplasmic pattern in nucleated epidermis. While, intra-nuclear pattern …
Identification Of Serum Proteomic Profile In Canine Lymphomas, Piyanoot Fonghem
Identification Of Serum Proteomic Profile In Canine Lymphomas, Piyanoot Fonghem
Chulalongkorn University Theses and Dissertations (Chula ETD)
Lymphoma is the third most commonly diagnosed, malignant hematopoietic tumor in dogs, and it is similar to human non-Hodgkin's lymphoma. Proteomics refer to the large-scale study of proteins, including their structures as well as their complex protein mixtures. It also involves identifying the changes in the protein isoforms and posttranslational modifications expressed by genetic material under defined specific conditions. This study was aimed at identifying the serum proteomic profiles of canine lymphoma compared with healthy dogs by using Tandem Mass Tag (TMT). Blood samples from twenty lymphoma and four healthy dogs were collected. All cases were categorized according to the …
Fatigue Study Of Cancellous And Cortical Stainless Steel Screws Used With Polymethymethacrylate And The Sop Plate System For The Immobilisation Of Vertebral Fracture And Luxation In Canine Cadavers, Mingrath Mekavichai
Chulalongkorn University Theses and Dissertations (Chula ETD)
Globally, vertebral fracture and luxation (VFL) are one of the most common neurological injuries in dogs and cats. The standard care for vertebral body stabilisation of canine VFL are screws with polymethylmethacrylate (PMMA) and the string of pearls (SOP) plate system fixation. The VFL with implants are usually exposed to force in a repetitive or cyclic loading fashion. Indeed, stress on the implant cyclically at a load significantly less than its ultimate tensile strength can cause fatigue failure, one of the major causes of implant breakage. This study aims to compare the failure mode of four different implants using a …
Effects And Mechanisms Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Type 1 And Type 2 On The Permeability And Viability Of Porcine Endometrial Epithelial Cells, Dran Rukarcheep
Chulalongkorn University Theses and Dissertations (Chula ETD)
Both PRRSV types 1 and 2 revealed the sign of reproductive disorders associated with a lesion at implantation sites. Impairment of maternal glandular endometrium cell integrity and function by PRRSV infection may impact a proper nourishing fetus. This research was aimed to examine the effects of PRRSV type 1 and type 2 directly on the viability and barrier function of the endometrium using porcine glandular endometrial epithelial cell culture (PEG). The comparison of the route and the strain of PRRSV infection coinciding with their virulent in reproductive epithelia were considered. PEG cells isolated from the 4-6 months old PRRSV-free-herd gilts …
Genetic Characterization Of S1 Gene Of Infectious Bronchitis Virus Isolated From Chickens In Thailand During 2014-2016, Sirorat Munyahongse
Genetic Characterization Of S1 Gene Of Infectious Bronchitis Virus Isolated From Chickens In Thailand During 2014-2016, Sirorat Munyahongse
Chulalongkorn University Theses and Dissertations (Chula ETD)
Infectious bronchitis (IB), caused by infectious bronchitis virus (IBV), is a highly contagious respiratory disease in chickens, causing significant economic losses in poultry industry worldwide. IBV has a high frequency of genetic mutation and recombination in its genome, particularly in the S1 gene, resulting in the emergence of several new IBV variants. This potentially affects the effectiveness of currently used IBV vaccine. Therefore, the continuous monitoring of the currently circulating IBV strains is essential. In this study, the genetic characteristic of IBV strains circulating in Thai chicken flocks during 2014-2016 was investigated by analysis of the complete S1 gene. A …
Difference Of Some Spermatozoa And Seminal Plasma Proteins In Good And Poor Freezability Boar Ejaculates, Janyaporn Rungruangsak
Difference Of Some Spermatozoa And Seminal Plasma Proteins In Good And Poor Freezability Boar Ejaculates, Janyaporn Rungruangsak
Chulalongkorn University Theses and Dissertations (Chula ETD)
The present study was performed to determine the differences of some seminal plasma proteins, i.e., glutathione peroxidase 5 (GPX5) and fibronectin 1 (FN1), and some spermatozoa proteins, i.e., triosephosphate isomerase (TPI) and ACRBP (acrosin binding protein), in the boar semen having good and poor freezability. The study was conducted by including 38 ejaculates in seminal plasma study and 32 ejaculates from spermatozoa study from 27 boars (Duroc=13, Landrace= 8, Yorkshire= 6). The semen was split into two portions. The first portion was centrifuged to separate the pellet of sperm from the seminal plasma. The samples were kept at −80 °C …
The Effects Of Hydroxyethyl Starch (Hes) On Quality Of Equine Sperm Following Cold Storage And Cryopreservation, Ornwanya Nuchanart
The Effects Of Hydroxyethyl Starch (Hes) On Quality Of Equine Sperm Following Cold Storage And Cryopreservation, Ornwanya Nuchanart
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study aimed at examining the effects of different concentrations of hydroxyethyl starch (HES) on quality of equine sperm following cold storage and cryopreservation. In experiment 1, the semen was collected from 6 stallions (3 replicates). The semen was diluted with extender for cold storage containing different concentrations of HES (2.5%, 5%, 10% and 15%). Extender without HES (0% HES) served as a control group. The temperature of extended semen was gradually decreased to 4 ºC. The quality of sperm in terms of progressive motility and viability was evaluated after cold storage for 6, 24 and 48 hours. This study …
Factors Influencing Colostrum Consumption Of Piglets And Levels Of Immunoglobulin G And Immunoglobulin A Specifically Against Ped Virus In Colostrum Of Sows, Patthawan Juthamanee
Factors Influencing Colostrum Consumption Of Piglets And Levels Of Immunoglobulin G And Immunoglobulin A Specifically Against Ped Virus In Colostrum Of Sows, Patthawan Juthamanee
Chulalongkorn University Theses and Dissertations (Chula ETD)
The aims of the present study was to investigate factors associated with colostrum consumption of the neonatal piglets and determine the concentrations of total immunoglobulins G (IgG) and immunoglobulins A (IgA) against porcine epidemic diarrhea (PED) virus in colostrum for sow. The study consisted of two parts: Part I, the study included data of colostrum consumption from 1,140 neonatal piglets from 80 sows. Factors associated with piglet’s colostrum consumption included body weight at birth of the piglet, birth order, birth interval, heart rate, blood oxygen saturation, rectal temperature at 24 h, gestation length, total born, born alive, sow body conditions …
Establishment Of Cardiac Lineage From Rabbit Embryonic And Induced Pluripotent Stem Cells, Praopilas Phakdeedindan
Establishment Of Cardiac Lineage From Rabbit Embryonic And Induced Pluripotent Stem Cells, Praopilas Phakdeedindan
Chulalongkorn University Theses and Dissertations (Chula ETD)
Rabbit is a promising model for studying myocardial infarction as several physiological and pathological conditions are resemble to humans. However, the information on establishment of rabbit pluripotent stem cells (PSC) and differentiation potential especially cardiac differentiation in this species has been limited. This research aimed at establishing and examining in vitro cardiogenesis of rabbit embryonic stem cells (ESC) and rabbit induced pluripotent stem cells (iPSC). In experiment 1, four cell lines of ESC derived from blastocyst stage embryos were cultured and characterized for their pluripotency in terms of gene and immunohistochemistry. They exhibited normal karyotypes (n=44). Embryoid body (EB) formation …
Development Of Inactivated Mycoplasma Gallisepticum Vaccine In Chickens, Arithat Limsatanun
Development Of Inactivated Mycoplasma Gallisepticum Vaccine In Chickens, Arithat Limsatanun
Chulalongkorn University Theses and Dissertations (Chula ETD)
Mycoplasma gallisepticum (MG) is the respiratory pathogen causing chronic respiratory disease (CRD) in poultry. More importantly, MG infection affects the economic losses of poultry industry due to decrease egg production and carcass quality. There are several procedures for MG monitoring and several commercial vaccines to prevent and control the MG infection. In Thailand, MG infection has been the one of major problems of poultry industry. The objectives of this study were to investigate the different molecular techniques for MG monitoring in the poultry farms and to prepare the inactivated vaccine or bacterin with Thai MG strain. There were 3 experiments …
Comparative Study Of Urinary N-Acetyl-Beta-D-Glucosaminidase Levels, Urinary Glycosaminoglycans And Plasma Glycosaminoglycans Levels In Cats With Feline Idiopathic Cystitis, Jeeranan Benjasiriwan
Comparative Study Of Urinary N-Acetyl-Beta-D-Glucosaminidase Levels, Urinary Glycosaminoglycans And Plasma Glycosaminoglycans Levels In Cats With Feline Idiopathic Cystitis, Jeeranan Benjasiriwan
Chulalongkorn University Theses and Dissertations (Chula ETD)
Comparative study was conducted to measure urinary N-acetyl-β-glucosaminidase (NAG), urinary glycosaminoglycans (GAGs) and plasma glycosaminoglycans (GAGs) in cats with feline idiopathic cystitis (FIC). A standard questionnaire was designed to gather information for all cats including signalment, characteristics, environment, type of food and management of the cats’ litter box to evaluate the risk factors for developing FIC. Blood and urine samples were collected from 19 clinically normal cats and 19 aged and sex matched cats with FIC. Concentration of urinary NAG, urinary GAGs and plasma GAGs were measured by colorimetric method. NAG index and GAGs-to-creatinine ratio were calculated. The results demonstrated …
The Role Of Triarylmethane-34 On Potassium Channel Kca3.1 In Doxorubicin-Induced Feline Kidney Cell Line, Paralee Nantarakchaikul
The Role Of Triarylmethane-34 On Potassium Channel Kca3.1 In Doxorubicin-Induced Feline Kidney Cell Line, Paralee Nantarakchaikul
Chulalongkorn University Theses and Dissertations (Chula ETD)
Study involved with potassium channel KCa3.1 blocker (triarylmethane-34; TRAM-34) in doxorubicin (DOX)-induced feline kidney cells was performed. The study was divided into two parts. In part I, subtoxic dose of TRAM-34 in feline kidney cell lines was studied to determine the cytotoxicity of TRAM-34 in feline kidney cells by cytotoxicity assay. In part II, feline kidney cell lines were incubated with appropriate dose and time of TRAM-34 pretreatment for 24 h or 30 min and post-treatment for 24 h condition in DOX-induced cell toxicity. Cytotoxicity assay, apoptosis and necrosis assay and KCa3.1 protein expression were measured. The cytotoxicity results indicated …
Serological Status Of Pcv2-Infected Swine Herds, Cherdpong Phupolphan
Serological Status Of Pcv2-Infected Swine Herds, Cherdpong Phupolphan
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study aimed to determine the dynamics of serological and virological profiles against Porcine Circovirus type 2 (PCV2) infection in Thai swine herds using the in-house indirect ELISA and real-time PCR techniques. A total of 10 swine herds in Thailand were divided into two groups according to their clinical history. Group A (n=5) had clinical signs of PCV2, and was routinely vaccinated by PCV2 vaccines with production loss more than 5%. Group B (n=5) had no clinical signs of PCV2 with production loss less than 5%. Group B consisted of two subgroups: PCV2-vaccinated herds (B-Vac, n=2) and non-vaccinated herds (B-non-Vac, …
Development Of Multiplex Rt-Pcr And Metagenomic Analyses Of Canine Infectious Respiratory Disease Complex Associated Viruses In Dogs, Chutchai Piewbang
Development Of Multiplex Rt-Pcr And Metagenomic Analyses Of Canine Infectious Respiratory Disease Complex Associated Viruses In Dogs, Chutchai Piewbang
Chulalongkorn University Theses and Dissertations (Chula ETD)
Canine infectious respiratory disease complex (CIRDC) viruses have been detected in dogs with respiratory illness. The CIRDC is associated with multiple factors depending on host susceptibility, environment and pathogens. Canine influenza virus (CIV), canine parainfluenza virus (CPIV), canine distemper virus (CDV), canine respiratory coronavirus (CRCoV), canine adenovirus type 2 (CAdV-2) and canine herpesvirus 1 (CaHV-1), are all associated with the CIRDC. Environmental factors serve as the one key point for infection. The transmission route can be via community-acquired infection (CAI) or hospital-associated infection (HAI), but the variable factors within these two routes are not well described. Moreover, novel pathogens that …
Development Of Dna Vaccine Against Porcine Epidemic Diarrhea Virus, Gun Temeeyasen
Development Of Dna Vaccine Against Porcine Epidemic Diarrhea Virus, Gun Temeeyasen
Chulalongkorn University Theses and Dissertations (Chula ETD)
Porcine epidemic diarrhea (PED) is a devastating enteric disease mainly affected to the sucking piglets which causing the severe economic losses in many countries worldwide including Thailand. To date, there have no satisfied commercial vaccine for PED. The clustering system based on the spike gene demonstrated 2 variant of PEDV, classical and pandemic variant, which the difference of these variants may be affected to the protective immunity between them. The objectives of this study were to investigate the genetic diversity of S gene of PEDV in Thailand and evaluate the efficacy of DNA vaccine which developed from Thai PEDV isolate. …
Detection And Distribution Of Enterotoxin And Adhesin Genes Of Staphylococcus Pseudintermedius Isolated From Dog, Human, And Environment, Nathita Phumthanakorn
Detection And Distribution Of Enterotoxin And Adhesin Genes Of Staphylococcus Pseudintermedius Isolated From Dog, Human, And Environment, Nathita Phumthanakorn
Chulalongkorn University Theses and Dissertations (Chula ETD)
To obtain the information of virulence factors associated with Staphylococcus pseudintermedius pathogenicity, host survival and adaptation, the study of staphylococcal enterotoxin (SE) genes and cell wall-associated (CWA) protein genes in S. pseudintermedius isolates from dogs, humans, and environment were performed. Human methicillin-resistant Staphylococcus pseudintermedius (MRSP) harbored the hightes number of SE genes at 12 out of 17 genes compared to those from dogs at 5 and environmental isolates at 3 gene types. For CWA protein genes or S. pseudintermedius surface proteins (sps), there was no an easy and fast method to detect them. Therefore, this study develop the multiplex PCR …
Dual Infection Between A Thai Isolate Highly Pathogenic Porcine Reproductive And Respiratory Syndrome Virus And Pandemic H1n1swine Influenza Virus In Weanling Pigs, Nattaphong Sangpratum
Dual Infection Between A Thai Isolate Highly Pathogenic Porcine Reproductive And Respiratory Syndrome Virus And Pandemic H1n1swine Influenza Virus In Weanling Pigs, Nattaphong Sangpratum
Chulalongkorn University Theses and Dissertations (Chula ETD)
After an introduction of pandemic H1N1 swine influenza virus (pdmH1N1 SIV) in the Thai swine farms, mild clinical outcome is observed and its role in porcine respiratory disease complex (PRDC) is questionable. A highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) has become endemic in the Thai swine farms. This study was conducted to determine the pathogenicity of the pdmH1N1 SIV when co-infected with a HP-PRRSV. Thirty-two weanling pigs from a free PRRSV, SIV, porcine circovirus type 2 (PCV-2) and Mycoplasma spp. commercial farm were randomly divided into 4 groups: Negative, PRRSV, SIV and Co-infection groups. Pigs in PRRSV …
Monitoring Of Antibiogram And Resistance Gene Profiles Among Escherichia Coli In Pig Production System, Kittitat Lugsomya
Monitoring Of Antibiogram And Resistance Gene Profiles Among Escherichia Coli In Pig Production System, Kittitat Lugsomya
Chulalongkorn University Theses and Dissertations (Chula ETD)
The antimicrobial resistant (AMR) bacteria in pig farms have been believed as an important source in food chain with public health concern. While some studies suggested transmission of AMR from pigs to humans may occur, but there was still needing to combine high resolution genomic data analysis with systematically collected epidemiological evidence to reconstruct patterns of AMR transmission between pigs and humans. The objectives of this study were to determine the occurrence and characterization the AMR phenotypes against 18 antimicrobials in pig producing system in both cross-sectional from fattening and longitudinal studies from newborn to slaughtering pigs and to evaluate …
Identification Of Porcine Circovirus Type 3 (Pcv3) In Thailand And Its Associated Syndrome, Roongtham Kedkovid
Identification Of Porcine Circovirus Type 3 (Pcv3) In Thailand And Its Associated Syndrome, Roongtham Kedkovid
Chulalongkorn University Theses and Dissertations (Chula ETD)
Porcine circovirus type 3 (PCV3) is a recently identified swine virus with currently unknown pathogenesis. To date, PCV3 has been found in many countries, indicating the wide spread of the virus. Therefore, there is an urgent need to clarify the detrimental role of PCV3. To better understand how PCV3 might influence the pig industry, various perspectives in swine production cycle should be considered. In this study, firstly, PCV3 was identified for the first time in Thailand. The study also showed that PCV3 has existed in the Thai pig population as early as 2005, suggesting that the virus might have long …
Immunophenotype, Molecular Diagnosis, Minimal Residual Disease, And Multidrug Resistance Protein Expression In Modified Cop, Modified Chop And Rescue Protocols Of Treated Canine Lymphomas, Sirintra Sirivisoot
Immunophenotype, Molecular Diagnosis, Minimal Residual Disease, And Multidrug Resistance Protein Expression In Modified Cop, Modified Chop And Rescue Protocols Of Treated Canine Lymphomas, Sirintra Sirivisoot
Chulalongkorn University Theses and Dissertations (Chula ETD)
Multicentric lymphoma is the most common hematopoietic tumors in dogs. Both B- or T-lymphocyte clones can originate to lymphoma. A gold standard method to differentiate each lymphocyte lineage is immunohistochemistry (IHC) by detecting specific proteins of B and T cell. A protein marker for T and B cells is CD3 and CD79a, respectively. However, another pan pre B-cell marker is Pax5 or B-cell specific activator protein. It is more specific and sensitive than CD79a because it expresses only in B-cell lymphoma cases. The first purpose of this research was to 1) determine Pax5 expression in canine lymphomas by IHC and …
Oral Fluid Samples Used For Prrs Diagnosis And Management, Yonlayong Woonwong
Oral Fluid Samples Used For Prrs Diagnosis And Management, Yonlayong Woonwong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Porcine reproductive and respiratory syndrome (PRRS) is an economically significant swine disease having a negative impact to the swine industry worldwide. Appropriate management strategies and diagnostic tests are crucial to successfully control PRRS virus (PRRSV). However, the application for oral fluid-based diagnosis has not been evaluated in the Thai farms previously. In this study, pre-extraction methods of oral fluids were evaluated to improve the PCR product yielded. Moreover, the oral fluids utilization for PRRSV monitoring during the gilt acclimatization and farrowing to nursery period was investigated. The results demonstrated that increasing sample volume might be a suitable simple method for …