Medicine and Health Sciences Commons^™

Predictive Performance Of Different Ntcp Techniques For Radiation-Induced Esophagitis In Nsclc Patients Receiving Proton Radiotherapy, Mei Chen, Zeming Wang, Shengpeng Jiang, Jian Sun, Li Wang, Narayan Sahoo, G Brandon Gunn, Steven J Frank, Cheng Xu, Jiayi Chen, Quynh-Nhu Nguyen, Joe Y Chang, Zhongxing Liao, X Ronald Zhu, Xiaodong Zhang

Faculty, Staff and Student Publications

This study aimed to compare the predictive performance of different modeling methods in developing normal tissue complication probability (NTCP) models for predicting radiation-induced esophagitis (RE) in non-small cell lung cancer (NSCLC) patients receiving proton radiotherapy. The dataset was composed of 328 NSCLC patients receiving passive-scattering proton therapy and 41.6% of the patients experienced ≥ grade 2 RE. Five modeling methods were used to build NTCP models: standard Lyman-Kutcher-Burman (sLKB), generalized LKB (gLKB), multivariable logistic regression using two variable selection procedures-stepwise forward selection (Stepwise-MLR), and least absolute shrinkage and selection operator (LASSO-MLR), and support vector machines (SVM). Predictive performance was internally …

Identification Of Functional Heterogeneity Of Carcinoma-Associated Fibroblasts With Distinct Il6-Mediated Therapy Resistance In Pancreatic Cancer, Kathleen M Mcandrews, Yang Chen, J Kebbeh Darpolor, Xiaofeng Zheng, Sujuan Yang, Julienne L Carstens, Bingrui Li, Huamin Wang, Toru Miyake, Pedro Correa De Sampaio, Michelle L Kirtley, Mariangela Natale, Chia-Chin Wu, Hikaru Sugimoto, Valerie S Lebleu, Raghu Kalluri

Faculty, Staff and Student Publications

The tumor microenvironment in pancreatic ductal adenocarcinoma (PDAC) involves a significant accumulation of fibroblasts as part of the host response to cancer. Employing single-cell RNA-sequencing, multiplex immunostaining, and several genetic mouse models, we identify carcinoma-associated fibroblasts (CAFs) with opposing functions in PDAC progression. Depletion of fibroblast activation protein (FAP)+ CAFs results in increased survival, in contrast to depletion of alpha smooth muscle actin (αSMA)+ CAFs that leads to decreased survival. Tumor-promoting FAP+ CAFs (TP-CAFs) and tumor-restraining αSMA+ CAFs (TR-CAFs) differentially regulate cancer-associated pathways and accumulation of Tregs. Improved efficacy of gemcitabine is observed when IL-6 is deleted from αSMA+ CAFs …

Structural Characterization Of Dicyanopyridine Containing Dnmt1-Selective, Non-Nucleoside Inhibitors, John R Horton, Sarath Pathuri, Kristen Wong, Ren Ren, Lourdes Rueda, David T Fosbenner, Dirk A Heerding, Michael T Mccabe, Melissa B Pappalardi, Xing Zhang, Bryan W King, Xiaodong Cheng

Faculty, Staff and Student Publications

DNMT1 maintains the parental DNA methylation pattern on newly replicated hemimethylated DNA. The failure of this maintenance process causes aberrant DNA methylation that affects transcription and contributes to the development and progression of cancers such as acute myeloid leukemia. Here, we structurally characterized a set of newly discovered DNMT1-selective, reversible, non-nucleoside inhibitors that bear a core 3,5-dicyanopyridine moiety, as exemplified by GSK3735967, to better understand their mechanism of inhibition. All of the dicyanopydridine-containing inhibitors examined intercalate into the hemimethylated DNA between two CpG base pairs through the DNA minor groove, resulting in conformational movement of the DNMT1 active-site loop. In …

The Experiences Of Collegiality By Early-Career Counselor Educators, Jessica M. Tyler, Malti Tuttle, Marina Naviaux, Sara Ellison

Journal of Counselor Preparation and Supervision

It is essential to develop a work culture that supports faculty needs, be it professional for promotion and tenure, or personal. This phenomenological study examined the lived experiences of collegiality by early-career counselor educator faculty members (CES) working in a CACREP institution. Relational support, expectations, administration, and doctoral experiences emerged as themes from these narratives. Implications for the field are discussed to address these experiences and provide recommendations to counselor education faculty and departments.

Intercampus Health Care Simulation Model For Development Of Students’ Interprofessional Socialization And Competency, Cody Marie Busch, Lynn Gilbertson, Colleen Gullickson, Elizabeth Laessig

Teaching and Learning in Communication Sciences & Disorders

Clinical education standards for speech-language pathology and nursing programs indicate the need for students to gain experience with interprofessional collaborative practice (ICP). However, colleges not affiliated with hospitals struggle to offer experiences between healthcare disciplines. The purpose of this paper is to present a case study as a model for interprofessional healthcare simulation that can be applied to schools that have limited access to a variety of professional programs and few complimentary medical specialties at their home institution. The partnership of a masters in speech-language pathology (MS-SLP) program at a public state sponsored institution and a bachelors of science in …

Perceptions And Effects Of Online Clinical Preparation Modules For First-Semester Speech-Language Pathology Graduate Students, Elizabeth M. Leatherman, Kris Pedersen

Teaching and Learning in Communication Sciences & Disorders

Students begin graduate school with varying backgrounds and experiences from their undergraduate program, which can result in different degrees of competence as they begin clinical work. This study investigated the effects of a series of seven online modules designed to review foundational clinical skills and enhance students’ clinical success. A cohort of 39 incoming graduate students at a midwestern university participated in the study and completed the modules during program orientation. Mixed methods were used to evaluate the effects and perceptions of the modules, including surveying students and the clinical faculty, comparing students’ clinical evaluations to those of previous students …

The Blended And Online Learning Design Fellows Program: Developing Teacher-Researchers In Communication Sciences And Disorders, Laurel Teller, Jena Mcdaniel, C. Melanie Schuele, Cynthia J. Brame

Teaching and Learning in Communication Sciences & Disorders

Developing skills in the scholarship of teaching and learning (SoTL) represents an important gap in doctoral training in Communication Sciences and Disorders. This study describes the Blended and Online Learning Design Fellows Program (BOLD) as a model for infusing SoTL into doctoral studies and for teaching graduate students how to develop online instructional modules. We first describe the structure of the yearlong BOLD Program. We then provide a case example of the development and SoTL evaluation of an online instructional module on the topic of intentional communication. We conclude with a discussion regarding how our study results inform future instructional …

The Use Of Interprofessional Education (Ipe) To Address Collaboration For Individualized Education Plans (Ieps): A Retrospective Study Of Occupational Therapy, Speech-Language Pathology, And Special Education Students’ Perceptions, Kristina Curro, Lisa Shooman, Sue Foo

Teaching and Learning in Communication Sciences & Disorders

The purpose of this project is to measure the effects of interprofessional education (IPE) on the perceptions of preservice professionals’ development of an Individualized Education Plan (IEP). The preservice professionals (PSPs) participants included master’s students from speech language pathology (SLP), occupational therapy (OT) and special education (SPED) programs (N = 78). The primary project goals were to examine the perceptions of the participants’ roles and knowledge on a multidisciplinary education team, their perceptions of the collaborative process, and their familiarity with the IEP development process. A mixed-methods, retrospective, cross-sectional investigation was used to obtain quantitative and qualitative outcomes. A …

A Proposed Framework For Peer Reviews, Sarah M. Ginsberg, Colleen F. Visconti

Teaching and Learning in Communication Sciences & Disorders

Journal manuscripts are peer reviewed with the hopes that the peer review will facilitate an editor's decision regarding the disposition of the article and that the feedback will be helpful to the authors in improving their manuscript. However peer reviews may not achieve these goals if they lack sufficient analysis and feedback. This article presents a domains of language for peer review (DoLPR) framework to improve depth and quality of peer reviews conducted on journal manuscripts. The framework was developed in hopes of improving the quality of peer reviews.

Innovative Changes To Study Abroad: Virtual Intercultural Learning During The Pandemic, Lata A. Krishnan, Lan Jin, Aletha Stahl, Sita Sreekumar, Saumya Sundaram, Manju Subrahmanian, Praveena Davis

Teaching and Learning in Communication Sciences & Disorders

Intercultural competence (ICC) is increasingly important for healthcare professionals, and study abroad (SA) programs have been one mechanism to increase students’ ICC. With SA programs being cancelled due to the pandemic, and qualitative literature supporting virtual intercultural learning (ICL), the purpose of this paper is to describe a novel approach towards “study abroad” without traveling abroad and evaluate participant gains in ICC with a virtual international ICL experience embedded into a graduate level pediatric audiology course. Nine U.S students and ten students at a partner institution in India were enrolled in the course. Comparison of pre- and post-IDI® scores showed …

Medication Therapy Management Implementation In Conjunction With A Rural Health Clinic In Minnesota: A Cqi Impact On Diabetes Metrics, Abigail Sirek Pharmd

Theses and Dissertations

The purpose of this continuous quality improvement project was to implement a pharmacist-led Medication Therapy Management (MTM) clinic in rural, central Minnesota and assess the impact on patients’ healthcare over a six-month period. Patients were identified through the clinic’s diabetes registry, which included patients 18 through 75 years old with a diagnosis of diabetes not meeting all five D5 goals, as recommended by the Minnesota Community Measurements, independently referred by the provider, or be identified by their insurance company as a patient that would benefit from an MTM visit. The D5 focuses on blood pressure and blood sugar control, appropriate …

Dataset Of Antemortem Tooth Loss And Carious Lesions At Deir El-Medina, Anne Austin

UMSL Datasets

These data are from observations on antemortem tooth loss and the presence of carious lesions from the site of Deir el-Medina, Egypt. The data were collected by Anne Austin and Mélie Louys as part of the mission of the Institut Français d’Archéologie Orientale at the site during the 2012-14 and 2019-20 field seasons. These data were derived from human remains found in Theban Tombs 6, 217, 290, and 298. They are estimated to date from 1550-945 BCE. These data include macroscopic observations from 32 mandibles and 44 maxillae consisting of 485 observable teeth and 1,052 observable tooth sockets. Any mandibles …

Synchronized Diaphragmatic Stimulation: A Case Report Of A Novel Extra-Cardiac Intervention For Chronic Heart Failure., Lee R Goldberg, Ana Jorbenadze, Tamaz Shaburishvilli, Michael Mirro Md, Marat Fudim, Michel Zuber, Simon F Stämpfli, Felix C Tanner, Paul Erne, John G Cleland

Parkview Heart Institute

Synchronized diaphragmatic stimulation (SDS) is a novel extra-cardiac device-based therapy for symptomatic heart failure with reduced ejection fraction. SDS provides imperceptible chronic stimulation of the diaphragm through a laparoscopically implanted system consisting of an implantable pulse generator and two sensing/stimulating leads affixed to the inferior surface of the diaphragm delivering imperceptible R-wave gaited pulses that alter intrathoracic pressure improving ventricular filling and cardiac output. We describe, in a man with a history of myocardial infarctions resulting in heart failure and persistent New York Heart Association Class III symptoms despite standard therapies, the successful implantation of SDS resulting in improved quality …

Design Of Topical Moxifloxacin Mucoadhesive Nanoemulsion For The Management Of Ocular Bacterial Infections, Ahmed Adel Ali Youssef, Ruchi Thakkar, Samir Senapati, Poorva H. Joshi

Faculty and Student Publications

Ocular bacterial infections can lead to serious visual disability without proper treatment. Moxifloxacin (MOX) has been approved by the US Food and Drug Administration as a monotherapy for ocular bacterial infections and is available commercially as an ophthalmic solution (0.5% w/v). However, precorneal retention, drainage, and low bioavailability remain the foremost challenges associated with current commercial eyedrops. With this study, we aimed to design a MOX-loaded nanoemulsion (NE; MOX-NE) with mucoadhesive agents (MOX-NEM) to sustain MOX release, as well as to overcome the potential drawbacks of the current commercial ophthalmic formulation. MOX-NE and MOX-NEM formulations were prepared by hot homogenization …

The International Cannabis Toolkit (Icanntoolkit): A Multidisciplinary Expert Consensus On Minimum Standards For Measuring Cannabis Use, Valentina Lorenzetti, Chandni Hindocha, Kat Petrilli, Paul Griffiths

Faculty and Student Publications

Background: The lack of an agreed international minimum approach to measuring cannabis use hinders the integration of multidisciplinary evidence on the psychosocial, neurocognitive, clinical and public health consequences of cannabis use. Methods: A group of 25 international expert cannabis researchers convened to discuss a multidisciplinary framework for minimum standards to measure cannabis use globally in diverse settings. Results: The expert-based consensus agreed upon a three-layered hierarchical framework. Each layer—universal measures, detailed self-report and biological measures—reflected different research priorities and minimum standards, costs and ease of implementation. Additional work is needed to develop valid and precise assessments. Conclusions: Consistent use of …

Medical School Watercooler Newsletter - June 1, 2022, Lindsey Lyle

Watercooler Newsletter

This is the June 1, 2022 edition of the Frederick P. Whiddon College of Medicine's newsletter - Watercooler.

Contents include:

• Medical students win awards at MASA conference
• Bauer to present on extracellular vesicle signaling
• Medical students begin summer research
• Meet a Med Student: Anna Shunnarah

Comparison Between Linear And Curved Arrangement Of Telescopic Attachment Used For 4 Implants Mandibular Overdenture: A Study Of Clinical Deformity Of Denture Base, Ahmed G. Ghozy, Osama M. Askar, Ahmed A. Habib

Mansoura Journal of Dentistry

Objective: The aim of this study was clinical evaluation of stresses developed in complete mandibular overdenture assisted by four implants using telescopic attachment placed in curved or linear configurations. Materials and Methods: Twelve completely edentulous participants were selected for the study and each patient received four implants. All patients were into 2 equal groups regarding implants position: The first group (Linear design): The four implants were installed at lateral incisor and canine areas. The second group (Curved design): the four implants were installed bilaterally in the interforaminal region of the mandible (at canine and second premolar areas). The implants were …

Early Orthopedic Correction Of Skeletal Class Ll Division 1 By Modified Bionator, Mohammad S. Qaisieh, Marwa S. Shamaa, Maher A. Fouda

Mansoura Journal of Dentistry

Objective: The purpose of this study was to evaluate the early treatment effects of the modified bionator with J-hook on soft tissue of growing patients with skeletal Class II division 1 malocclusion. Materials and Methods: This investigation was conducted on 12 patients. All the patients had the following criteria: skeletal Class II division 1 due to mandibular retrusion with maxillary excess. Age ranged from 8-12 (mixed dentition and early permanent dentition). ANB° was more than 6°. Overjet was more than 5 mm. Lateral cephalometric radiographs, photographs and study casts were obtained before and after treatment. The collected data were analyzed …

Study On The Factors Influencing Return To Work Within First Six Months Among Blue Collar Workers With Hand Injury In India, Nguyen Khac Tuan

Manipal College of Health Professions, Manipal Theses and Dissertations

No abstract provided.

Micro-Rna Analysis Of Extracellular Vesicles Secreted By Alveolar Macrophages And Epithelial Cells In Response To Cadmium, Anisha Bhakta

Biotechnology Theses

Inhalation of cadmium (Cd) has been an environmental health concern with the increase in industrial activities and smoking. Cd exposure is known to affect several organs such as lungs, kidneys, and liver. In this study, we wanted to understand if Cd inhalation exposure can affect the lungs and other organs in the body. Exosomes are extracellular vesicles secreted by all living cells and are known to carry toxicants between organs. We investigated whether Cd exposure could affect exosome biogenesis and their composition. Cd exposure did not affect the viability of A549, a lung epithelial cell line and macrophages derived from …

The Rd-Connect Genome-Phenome Analysis Platform: Accelerating Diagnosis, Research, And Gene Discovery For Rare Diseases., Steven Laurie, Davide Piscia, Leslie Matalonga, Alberto Corvó, Marcos Fernández-Callejo, Carles Garcia-Linares, Carles Hernandez-Ferrer, Cristina Luengo, Inés Martínez, Anastasios Papakonstantinou, Daniel Picó-Amador, Joan Protasio, Rachel Thompson, Raul Tonda, Mònica Bayés, Gemma Bullich, Jordi Camps-Puchadas, Ida Paramonov, Jean-Rémi Trotta, Angel Alonso, Marcella Attimonelli, Christophe Béroud, Virginie Bros-Facer, Orion J Buske, Andrés Cañada-Pallarés, José M Fernández, Mats G Hansson, Rita Horvath, Julius O B Jacobsen, Rajaram Kaliyaperumal, Séverine Lair-Préterre, Luana Licata, Pedro Lopes, Estrella López-Martín, Deborah Mascalzoni, Lucia Monaco, Luis A Pérez-Jurado, Manuel Posada De La Paz, Jordi Rambla, Ana Rath, Olaf Riess, Peter N Robinson, David Salgado, Damian Smedley, Dylan Spalding, Peter A C 'T Hoen, Ana Töpf, Irina Zaharieva, Holm Graessner, Ivo G Gut, Hanns Lochmüller, Sergi Beltran

Faculty Research 2022

Rare disease patients are more likely to receive a rapid molecular diagnosis nowadays thanks to the wide adoption of next-generation sequencing. However, many cases remain undiagnosed even after exome or genome analysis, because the methods used missed the molecular cause in a known gene, or a novel causative gene could not be identified and/or confirmed. To address these challenges, the RD-Connect Genome-Phenome Analysis Platform (GPAP) facilitates the collation, discovery, sharing, and analysis of standardized genome-phenome data within a collaborative environment. Authorized clinicians and researchers submit pseudonymised phenotypic profiles encoded using the Human Phenotype Ontology, and raw genomic data which is …

An Evaluation Of The Effect Of The Oxycontin Reformulation On Unintentional Fatal And Nonfatal Overdose, Daniel C. Beachler, Kelsey Hall, Renu Garg, Geetanjoli Banerjee, Ling Li, Luke Boulanger, Huseyin Yuce, Alexander M. Walker

Applied Sciences, Technology and Education Faculty Publications

Objectives:

OxyContin was reformulated with a polyethylene oxide matrix in August 2010 to reduce the potential for intravenous abuse and for abuse by insufflation. The objective of this study was to evaluate the impact of OxyContin's reformulation on overdose (OD) risk for individuals dispensed OxyContin in comparison to those dispensed other opioids under regular care.

Materials and Methods:

Three national insurance databases with National Death Index linkage identified OD in individuals with any dispensing of OxyContin or a primary comparator opioid (extended release morphine, transdermal fentanyl, or methadone) between July 2008 through September 2015. A difference-in-differences design was used to …

Ethoss Versus Mineralized Plasmatic Matrix For Maxillary Lateral Sinus Lifting With Simultaneous Implant Placement, Abdullwahab A. Al Khadher, Sally E. Abdelsameaa, Wael M. Ahmed

Mansoura Journal of Dentistry

Objective: The aim of this study was to compare the effect of Ethoss and Mineralized Plasmatic Matrix (sticky bone) for maxillary lateral sinus lifting with simultaneous implant placement. Patients and methods: This study included 20 patients with missed maxillary posterior teeth and they were treated by lateral maxillary sinus lift with Simultaneous Implant Placement. The patients were equally and randomly classified into two equal groups. Group I used Ethoss for bone grafting (n=10) while group II used Mineralized Plasmatic Matrix (MBM) (n=10). All patient was evaluated clinically (the stability of the dental implant, the depth of pocket around the implant, …

2022-06-01 Michiganoverview_Covidbedppe, Michigan Department Of Health & Human Services

Michigan COVID-19 Hospital Data

Contains the tables Statewide Hospital Capacity Report for COVID-19, COVID-19 Metrics, Statewide Hospital Available PPE Tracking for COVID-19, as well as the tables PPE Days On Hand and Patient Census.

2022-06-01_Michiganoverview_Covidstats, Michigan Department Of Health & Human Services

Cumulative Michigan COVID-19 Data

The COVID Overview page was obtained from Michigan's main COVID-19 page: https://www.michigan.gov/coronavirus/stats.

The State of Michigan is working around the clock to test and treat COVID-19 patients. Below is the most recent data healthcare experts across the state have compiled. COVID case and testing data will be posted once a week on Wednesdays.

This report is provisional and subject to change. City of Detroit and Wayne County are reported separately. MDHHS began tracking probable cases April 5, 2020. FCI indicates federal correctional institute. MDOC indicates Michigan Department of Corrections. Diagnostic tests, including nucleic acid amplification/real-time polymerase chain reaction (NAAT/RT-PCR) …

2022-06-01_Michiganoverview_Covidcounts, Michigan Department Of Health & Human Services

Michigan COVID-19 Confirmed Cases by Date of Onset Data

Data was downloaded as an Excel spreadsheet which appeared in the Public Use Datasets section under the Cumulative Data link of Michigan's Coronavirus webpage, under the url https://www.michigan.gov/coronavirus/0,9753,7-406-98163_98173---,00.html.

Cases are aggregated by the date of onset of COVID-19 symptoms, if known, otherwise by laboratory specimen date if known, otherwise by case referral date. Deaths are aggregated by the date of death if known, otherwise by the last date of a change to the case status.

Use Of Micro-Ct To Examine Effects Of Heat On Coronal Obturation, Ivy Shen, Joan Daniel, Kali Vo, Chul Ahn, Carolyn Primus, Takashi Komabayashi

Dental Medicine Faculty Publications

Purpose: The aim of this study was to categorize the effects of heat on coronal obturation with gutta-percha and sealer using X-ray micro-computed tomography (micro-CT).
Methods: Ten single-rooted, extracted human teeth were shaped using ProTaper NEXT files to size X5 (#50/Taper 6%) with 2.5% NaOCl irrigation. A single ProTaper NEXT X5 gutta-percha point was then inserted with epoxy resin (AH Plus) or tricalcium silicate (EndoSequence BC) sealer (n = 5/group), and cut at the cemento-enamel junction. The teeth were scanned using micro-CT (SkyScan1272) to obtain 11 sagittal 2-D images. Three calibrated raters categorized the coronal 0.5 mm of the …

The Role Of Reported Affective Symptoms And Anxiety In Recovery Trajectories After Sport-Related Concussion, Bernadette A. D'Alonzo, Abigail C. Bretzin, Douglas J. Wiebe, Ivy League–Big Ten Epidemiology Of Concussion Study Investigators, Arthur C. Maerlender, Cary R. Savage

Center for Brain, Biology, and Behavior: Faculty Publications

Background: There is growing awareness and clinical interest in athletes with affective symptoms after sport-related concussion (SRC), as these symptoms may contribute to overall symptoms and represent a modifiable risk factor of longer recovery. However, evidence of their effects on the entire return-to-play (RTP) trajectory, particularly among women and men, is limited.

Purpose/Hypothesis: To examine the relationship between affective symptom reporting and RTP progression after SRC among a cohort of Division 1 student-athletes. We hypothesized that those endorsing affective symptoms, specifically nervous-anxious symptoms, spend more time in RTP progression and recovery.

Study Design: Cohort study; Level of evidence, 3.

Methods: …

Age-Dependent White Matter Disruptions After Military Traumatic Brain Injury: Multivariate Analysis Results From Enigma Brain Injury, Heather C. Bouchard, Delin Sun, Emily L. Dennis, Mary R. Newsome, Seth G. Disner, Jeremy Elman, Annelise Silva, Carmen Velez, Andrei Irimia, Nicholas D. Davenport, Scott R. Sponheim, Carol E. Franz, William S. Kremen, Michael J. Coleman, M. Wright Williams, Elbert Geuze, Inga K. Koerte, Martha E. Shenton, Maheen M. Adamson, Raul Coimbra, Gerald Grant, Lori Shutter, Mark S. George, Ross D. Zafonte, Thomas W. Mcallister, Murray B. Stein, Paul M. Thompson, Elisabeth A. Wilde, David F. Tate, Aristeidis Sotiras, Rajendra A. Morey

Center for Brain, Biology, and Behavior: Faculty Publications

Mild Traumatic brain injury (mTBI) is a signature wound in military personnel, and repetitive mTBI has been linked to age-related neurogenerative disorders that affect white matter (WM) in the brain. However, findings of injury to specific WM tracts have been variable and inconsistent. This may be due to the heterogeneity of mechanisms, etiology, and comorbid disorders related to mTBI. Non-negative matrix factorization (NMF) is a data-driven approach that detects covarying patterns (components) within high-dimensional data. We applied NMF to diffusion imaging data from military Veterans with and without a self-reported TBI history. NMF identified 12 independent components derived from fractional …

3d Patient-Derived Tumor Models To Recapitulate Pediatric Brain Tumors In Vitro., Min D Tang-Schomer, Harshpreet Chandok, Wei-Biao Wu, Ching C Lau, Markus J Bookland, Joshy George

Faculty Research 2022

Brain tumors are the leading cause of cancer-related deaths in children. Tailored therapies need preclinical brain tumor models representing a wide range of molecular subtypes. Here, we adapted a previously established brain tissue-model to fresh patient tumor cells with the goal of establishing3D in vitro culture conditions for each tumor type.Wereported our findings from 11 pediatric tumor cases, consisting of three medulloblastoma (MB) patients, three ependymoma (EPN) patients, one glioblastoma (GBM) patient, and four juvenile pilocytic astrocytoma (Ast) patients. Chemically defined media consisting of a mixture of pro-neural and pro-endothelial cell culture medium was found to support better growth than …