Medicine and Health Sciences Commons^™

Sars-Cov-2 Neuroinvasion, Inflammatory Neurodegeneration And Alzheimer's Disease, Yuhai Zhao, Walter J. Lukiw

School of Medicine Faculty Publications

No abstract provided.

Editorial: Nk/T-Cell Lymphoma: Biology, Prognostics, Prediction, And Treatment, Jean El Cheikh, Khodr Terro, Layal Sharrouf, Anjali Mishra, Marcos De Lima

Department of Medical Oncology Faculty Papers

No abstract provided.

Directed Evolution Of Pd-L1-Targeted Affibodies By Mrna Display, Brian J Grindel, Brian J Engel, Justin N Ong, Anupallavi Srinivasamani, Xiaowen Liang, Niki M Zacharias, Robert C Bast, Michael A Curran, Terry T Takahashi, Richard W Roberts, Steven W Millward

Faculty, Staff and Student Publications

Therapeutic monoclonal antibodies directed against PD-L1 (e.g., atezolizumab) disrupt PD-L1:PD-1 signaling and reactivate exhausted cytotoxic T-cells in the tumor compartment. Although anti-PD-L1 antibodies are successful as immune checkpoint inhibitor (ICI) therapeutics, there is still a pressing need to develop high-affinity, low-molecular-weight ligands for molecular imaging and diagnostic applications. Affibodies are small polypeptides (∼60 amino acids) that provide a stable molecular scaffold from which to evolve high-affinity ligands. Despite its proven utility in the development of imaging probes, this scaffold has never been optimized for use in mRNA display, a powerful

Fusobacterium Necrophorum Septicemia Secondary To An Ovarian Abscess: A Case Report., Amanda Morrall, Uwe Schmidt

Ambulatory and Primary Care Articles

Fusobacterium necrophorum is part of the normal oropharyngeal flora and can result in a life-threatening systemic infection known as Lemierre's syndrome. A rare presentation of F. necrophorum infection is seen in the female genital tract and is typically due to obstetric infections. Here we present a unique case of F. necrophorum without traditional features of Lemierre's syndrome with the female genital tract as a primary site. A 50-year-old female presents with a two-month history of nausea, vomiting, abdominal pain, and weight loss. She ultimately developed bilateral lower extremity necrotizing fasciitis, colonic perforation, and a left chest wall abscess. Blood and …

Inflammatory Breast Cancer: The Cytokinome Of Post-Mastectomy Wound Fluid Augments Proliferation, Invasion, And Stem Cell Markers, Alshaimaa Tarek, Shrouk Khalaf El-Sayed, Wendy A Woodward, Mohamed El-Shinawi, Jon Mark Hirshon, Mona Mostafa Mohamed

Faculty, Staff and Student Publications

Inflammatory breast cancer (IBC) is an aggressive phenotype with a high recurrence and low survival rate. Approximately 90% of local breast cancer recurrences occur adjacent to the same quadrant as the initial cancer, implying that tumor recurrence may be caused by residual cancer cells and/or quiescent cancer stem cells (CSCs) in the tumor. We hypothesized that wound fluid (WF) collected after modified radical mastectomy (MRM) may activate cancer cells and CSCs, promoting epithelial mesenchymal transition (EMT) and invasion. Therefore, we characterized the cytokinome of WF drained from post-MRM cavities of non-IBC and IBC patients. The WF of IBC patients showed …

Development And Evaluation Of A Remote Training Strategy For The Implementation Of Mental Health Evidence-Based Practices In Rural Schools: Pilot Study Protocol., Ricardo Eiraldi, Barry Mccurdy, Muniya S Khanna, Jessica Goldstein, Rachel Comly, Jennifer Francisco, Laura E Rutherford, Tara Wilson, Kathryn Henson, Thomas Farmer, Abbas F Jawad

PCOM Scholarly Works

BACKGROUND: An increasing number of schools in rural settings are implementing multi-tier positive behavioral interventions and supports (PBIS) to address school-climate problems. PBIS can be used to provide the framework for the implementation of evidence-based practices (EBPs) to address children's mental health concerns. Given the large service disparities for children in rural areas, offering EBPs through PBIS can improve access and lead to better long-term outcomes. A key challenge is that school personnel need technical assistance in order to implement EBPs with fidelity and clinical effectiveness. Providing ongoing on-site support is not feasible or sustainable in the majority of rural …

Isolation And Rna Sequencing Of Single Nuclei From Drosophila Tissues, Colleen N Mclaughlin, Yanyan Qi, Stephen R Quake, Liqun Luo, Hongjie Li

Center on Aging Staff Publications

Many insect cells are encapsulated within the exoskeleton and cannot be dissociated intact, making them inaccessible to single-cell transcriptomic profiling. We have used single-nucleus RNA sequencing to extract transcriptomic information from multiple Drosophila tissues. Here, we describe procedures for the (1) dissociation of single nuclei, (2) isolation of single nuclei using two popular cell sorters, and (3) preparation of libraries for Smart-seq2 and 10× Genomics. This protocol enables generation of high-quality transcriptomes from single nuclei and can be applied to other species.

For complete details on the use and execution of this protocol, please refer to McLaughlin et al. (2021) …

Experience Of Telemedicine In Gastroenterology Out-Patient Practice During The Covid-19 Pandemic: Experiences From A Tertiary-Care Hospital In A Developing Country, Anjiya Shaikh, Maria Khan, Faisal Wasim Ismail

Department of Medicine

Objective: Telemedicine is being widely implemented in the COVID-19 pandemic to avoid infection risk. However, its effectiveness has not been evaluated, especially in developing countries, where it is invaluable for healthcare access. This study assesses physicians' and patients' perspectives of the usefulness and challenges of telemedicine in the gastroenterology department to identify its pitfalls.
Methods: A cross-sectional telephonic survey was conducted on patients presenting to the gastroenterology department at a tertiary care hospital in Pakistan. An online survey was sent to physicians in the department.
Results: A total of 160 patients participated, with a mean age 49.8 years, and 42.8% …

Protocol To Analyze And Quantify Protein-Methylated Rna Interactions In Mammalian Cells With A Combination Of Rna Immunoprecipitation And Nucleoside Mass Spectrometry, Ning Tsao, Jennifer M Soll, Nima Mosammaparast

2020-Current year OA Pubs

Cellular RNAs are modified by both physiological factors and exogenous agents, such as methyl methanesulfonate (MMS). However, techniques for analyzing how proteins may interact with these modified RNAs are limited. Here, we provide a protocol combining RNA immunoprecipitation (RIP) with mass spectrometry (MS) to analyze the methylation state of the RNAs bound by Flag-tagged proteins in mammalian cells. The approach is highly quantitative and can simultaneously detect several methylated nucleosides in a single experiment. For complete details on the use and execution of this protocol, please refer to Tsao et al. (2021).

Evaluation Of Long-Chain Fatty Acid Respiration In Neonatal Mouse Cardiomyocytes Using Seahorse Instrument, Aude Angelini, Xinchun Pi, Liang Xie

Faculty, Staff and Students Publications

Metabolic switches play a critical role in the pathophysiology of cardiac diseases, including heart failure. Here, we describe an assay for long-chain fatty acid oxidation in neonatal mouse cardiomyocytes by using a SeaHorse Flux Analyzer (Agilent). This protocol is a simplified but robust adaptation of the standard protocol that enables metabolic measurements in cells isolated from transgenic mouse models, which can be timesaving and informative. Cell isolation and culture represent a critical point that may require bench optimization.

For complete details on the use and execution of this protocol, please refer to Angelini et al. (2021).

Imaging Peripheral Nerve Micro-Anatomy With Muse, 2d And 3d Approaches, Chaitanya Kolluru, Austin Todd, Aniruddha R Upadhye, Yehe Liu, Mikhail Y Berezin, Farzad Fereidouni, Richard M Levenson, Yanming Wang, Andrew J Shoffstall, Michael W Jenkins, David L Wilson

2020-Current year OA Pubs

Understanding peripheral nerve micro-anatomy can assist in the development of safe and effective neuromodulation devices. However, current approaches for imaging nerve morphology at the fiber level are either cumbersome, require substantial instrumentation, have a limited volume of view, or are limited in resolution/contrast. We present alternative methods based on MUSE (Microscopy with Ultraviolet Surface Excitation) imaging to investigate peripheral nerve morphology, both in 2D and 3D. For 2D imaging, fixed samples are imaged on a conventional MUSE system either label free (via auto-fluorescence) or after staining with fluorescent dyes. This method provides a simple and rapid technique to visualize myelinated …

Spinal Tumors Operated At University Clinical Centre Of Kosova In The Periods 2015-2020, Besnik Elshani

International Journal of Business and Technology

Introduction: Spinal tumors are abnormal masses that grow uncontrollably from normal cell growth and are mainly located around the spinal cord and column. Primary tumors originate in the spine or spinal cord while secondary or metastatic tumors appear in other parts of the body and spread from there to the spine. Spinal tumors are referred to depending on the region where they appear (Cervical, thoracic lumbar and sacrum), as well as their location on the spine. Purpose: The purpose of this research was the analysis of spinal tumors operated and treated at the University Clinical Center of Kosovo in the …

A Review On Active Food Packing As Innovation Strategies For The Future, Violeta Lajqi

International Journal of Business and Technology

Due to the dynamic life, consumers are constantly seeking to increase the shelf life of packaged food products, encouraging scientists in various fields to develop innovative packaging that meets the demands of consumers and at the same time producers. The purpose of this paper is to review the latest trends in the development of active packaging (AP) for foods. Given the specific properties of each type of food as well as the storage and distribution conditions, the choice of packaging material with accompanying elements requires a careful approach to each type of food. The knowledge of packaging materials, food properties, …

Evaluation A Renal Function Of Patients With Medication- Overuse Headache (Moh), Drita Yzeiri Havziu

International Journal of Business and Technology

Excessive drug use causes Medication-overuse headache (MOH) which can be manifested with chronic daily headaches, occurring monthly 15 or more days when the medicament is used redundantly for more than three months. Recent studies concerning the epidemiology of drug- induced disorders suggest that an increased risk of nephrotoxicity appears in a group of patients who abuse NSAIDs. The aim is to confirm the early phase of nephrotoxicity in patients with (MOH), who were treated with NSAIDs in combination with other drugs (analgesics, triptans, and antidepressants) and compared patients treated only with Diclofenac, Piroxicam, Ketoprofen, Paracetamol, Ibuprofen, and Celecoxib. Besides conventional …

2022-06-16_Michiganoverview_Misc, Michigan Department Of Health & Human Services

Michigan COVID-19 MIS-C Data

The MIS-C Data page was obtained from Michigan's COVID-19 data page: https://www.michigan.gov/coronavirus/stats/mis-c-reporting.

This report is created by the Michigan Department of Health & Human Services from data self-reported by health care providers and local health departments. Data is updated on the first and third Thursdays of the month.

Multisystem Inflammatory Syndrome in Children (MIS-C) is a condition in children and adolescents under 21 years of age where multiple organ systems become inflamed or dysfunctional which occurs in association with illness. Children with MIS-C may have a fever and various symptoms, including abdominal (gut) pain, vomiting, diarrhea, neck pain, rash, …

The Impact Of Background Noise On The Communicative Experience Of People With Mild To Moderate Aphasia: A Qualitative Study, Riley Robertson Hegewald

Theses and Dissertations

This study examined how retelling stories with a variety different background noise conditions affected the communicative experiences of people with aphasia (PWA). Participants included 11 adults with mild to moderate aphasia and 11 age- and gender-matched controls. Participants participated in a semi-structured interview immediately following the experimental measure where they were asked open-ended questions regarding their experience retelling stories and how those experiences related to their everyday lives. Results revealed three themes related to how participants responded to communicating in noise: (a) cognitive reactions, (b) emotional reactions, and (c) social reactions. The findings suggest that PWA exert more effort when …

The Analysis Of Omission And Substitution Errors In Various Language Tasks In Bilingual Children, Macy Whiting

Theses and Dissertations

As the population of Spanish-English bilingual children in the United States steadily grows, the importance of accurately assessing and diagnosing developmental language disorder (DLD) has also grown. Understanding a child's underlying language learning system is critical in this process. Several studies have demonstrated the efficacy of nonword repetition (NWR) tasks in bilingual children across a wide range of development, however there is little information regarding guidelines for interpretation of NWR performance or analyzing error patterns. This study was conducted to address these gaps in the research. A sample of 26 Spanish-English bilingual school aged children (6;0-9;4) were administered English and …

How Different Pre-Existing Mental Disorders And Their Co-Occurrence Affects Covid-19 Clinical Outcomes? A Real-World Data Study In The Southern United States, Shan Qiao Ph.D., Jiajia Zhang Ph.D., Shujie Chen, Bankole Olatosi Ph.D., Suzanne Hardeman, Meera Narasimhan, Larisa Bruner, Abdoulaye Diedhiou, Cheryl Scott, Ali Mansaray, Sharon Weissman, Xiaoming Li Ph.D.

Faculty Publications

Background: Although a psychiatric history might be an independent risk factor for COVID-19 infection and mortality, no studies have systematically investigated how different clusters of pre-existing mental disorders may affect COVID-19 clinical outcomes or showed how the coexistence of mental disorder clusters is related to COVID-19 clinical outcomes.

Methods: Using a retrospective cohort study design, a total of 476,775 adult patients with lab-confirmed and probable COVID-19 between March 06, 2020 and April 14, 2021 in South Carolina, United States were included in the current study. The electronic health record data of COVID-19 patients were linked to all payer-based claims data …

Community Health Worker Program - Substance Use Disorder In Pregnancy, Alicia Claire Kletter Dnp

Doctor of Nursing Practice (DNP) Projects

Background Substance use disorder (SUD) in pregnancy is a concern for mother, baby and community. Healthcare policies are increasingly calling for diversification of the workforce to meet healthcare needs. Community health workers (CHWs) are understood from the literature to be effective at bridging the gap between patient and healthcare provider, especially for vulnerable populations. Local Problem California’s Central Valley is majority Latinx and faces challenges from poverty, lack of medical resources, unemployment and SUD at rates which are higher than other communities. Combine SUD with pregnancy and the need for robust social connections, psychological support, and physical healthcare become even …

Microrna, The Innate-Immune System And Sars-Cov-2, James M. Hill, Walter J. Lukiw

School of Medicine Faculty Publications

The single-stranded viral RNA (ssvRNA) known as the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that causes COVID-19 can be effectively inactivated by a number of natural ribonucleic acid-based host cell defenses. One of the most important of these defenses includes the actions of a class of small non-coding RNAs (sncRNAs) known as microRNAs (miRNAs). Via base-pair complementarity miRNAs are capable of specifically targeting ssvRNA sequences such as SARS-CoV-2 promoting its inactivation and neutralization. RNA-sequencing and bioinformatics analysis indicate that multiple naturally-occurring human miRNAs have extensive complementarity to the SARS-CoV-2 ssvRNA genome. Since miRNA abundance, speciation, and complexity vary significantly …

Examining How Congruence In And Satisfaction With Dyadic Care Type Appraisal Contribute To Quality Of Life In Heart Failure Care Dyads, Elliane Irani, Seunghee Margevicius

Faculty Scholarship

AIMS: Given the complexity of heart failure (HF) management, persons with HF and their informal caregivers often engage in dyadic illness management. It is unknown how congruent appraisal of dyadic HF care type is associated with dyadic health. Our aim was to examine how congruence in and satisfaction with appraisal of dyadic HF care type contribute to quality of life (QOL) for dyads. METHODS AND RESULTS: This is a secondary analysis of cross-sectional data on 275 HF care dyads (patients 45.1% female, caregivers 70.5% female). Congruent appraisal and satisfaction were assessed using the Dyadic Symptom Management Type instrument. Quality of …

Quality Improvement In A Hospital Setting: Central Line-Associated Bloodstream Infections At An Acute Care Hospital In South Texas, Hoang Anh T. Nguyen, Jose E. Campo Maldonado

MEDI 9331 Scholarly Activities Clinical Years

Background: Central line-associated infections (CLABSI) are life-threatening nosocomial infections that are associated with significant increase in healthcare expenditure, prolonged stays, and risk of mortality.

Objective: To identify outcome trends and risk factors that contribute to incidence of CLABSI at an 866-bed acute care hospital in South Texas.

Methods: A retrospective chart review was conducted on 38 patients who met the National Healthcare Safety Network criteria for CLABSI during the period of January 2021 to March 2022

Results: There was a total of thirty-two CLABSIs in 2021. Hospital-wide CLABSI rate was 1.76 hospital-wide per 1,000-line days. Half of cases were COVID-19 …

Houseplants As Mental Health Supports For Dorm Occupants During The Lockdown Period At Portland State University, Brittani Wallsten

Anthós

In this study, students who lived in dorms around the lockdown period of Portland State University, March 2020—September 2021, were interviewed about their experience and how their houseplants affected their mental health. This was done via in-person interviews and an online focus group. Houseplants were found to support students’ mental health by encouraging a regular routine, providing opportunities for responsibility, adding aesthetic value, and serving as a general indicator of mental health. All of the participants recommended houseplants as a mental health support to their fellow students.

Serum Metabolomics Reveals Distinct Profiles During Ischemia And Reperfusion In A Porcine Model Of Myocardial Ischemia–Reperfusion, Eric Goetzman, Zhenwei Gong, Dhivyaa Rajasundaram, Ishan Muzumdar, Traci Goodchild, David Lefer, Radhika Muzumdar

School of Medicine Faculty Publications

Acute myocardial infarction (MI) is one of the leading causes of death worldwide. Early identification of ischemia and establishing reperfusion remain cornerstones in the treatment of MI, as mortality and morbidity can be significantly reduced by establishing reperfusion to the affected areas. The aim of the current study was to investigate the metabolomic changes in the serum in a swine model of MI induced by ischemia and reperfusion (I/R) injury, and to identify circulating metabolomic biomarkers for myocardial injury at different phases. Female Yucatan minipigs were subjected to 60 min of ischemia followed by reperfusion, and serum samples were collected …

The Passive Approach: How Academically Motivated Students Approach Their Mental Health., Megan E. Mccoy

Anthós

The research utilizes qualitative interviews and focus groups to understand how academically motivated students approach their mental health. Mental health is defined as “emotional, psychological, and social well-being,” by the Center for Disease Control and Prevention and is becoming a more significant concern in collegiate student populations. At Portland State University, the effects of mental health concerns on students are nearly double that of students around the U.S (Community Commons, n.d.). Academically motivated students are a group who seemingly strive for success and likely experience high levels of stress daily. The study found that students often take a passive approach …

Covid's Impact On Nurses & Their Labor Market, Joshua Stanfill

University Honors Theses

The Covid pandemic has swept across the globe impacting many different kinds of people in many different ways. Businesses and workers experienced changes in how their respective labor markets operated with new incentives. One group of laborers that experienced quite a significant impact are nurses working through the Covid pandemic. The pandemic created new incentives for nurses that heavily impacted their labor market. Through a literature review of scientific studies examining the impacts of working through a pandemic on nurses, I discovered that this was causing psychological and emotional damage to nurses. Covid spurred on an already occurring nursing shortage …

Facilitators And Barriers To Farmers’ Market Use In A Rural Area, Soghra Jarvandi, Kristen Johnson, Karen Franck

Journal of Extension

Farmers’ markets may improve access to healthful foods in rural areas. Our objective was to identify facilitators and barriers to farmers’ market use in a rural county. We collected data via surveys, focus group sessions, and key informant interviews. Study participants identified the two existing farmers’ markets as community assets. Barriers to use farmers’ markets included inconvenient market hours, not accepting nutrition assistance program benefits, limited transportation, and limited variety. Interventions to improve food access should include ways to meet the needs of specific populations such as low-income residents and residents living in outlying areas without farmers’ markets.

Ros And Mirna Dysregulation In Ovarian Cancer Development, Angiogenesis And Therapeutic Resistance, David C Stieg, Yifang Wang, Ling-Zhi Liu, Bing-Hua Jiang

Kimmel Cancer Center Faculty Papers

The diverse repertoires of cellular mechanisms that progress certain cancer types are being uncovered by recent research and leading to more effective treatment options. Ovarian cancer (OC) is among the most difficult cancers to treat. OC has limited treatment options, especially for patients diagnosed with late-stage OC. The dysregulation of miRNAs in OC plays a significant role in tumorigenesis through the alteration of a multitude of molecular processes. The development of OC can also be due to the utilization of endogenously derived reactive oxygen species (ROS) by activating signaling pathways such as PI3K/AKT and MAPK. Both miRNAs and ROS are …

Systematic Review And Meta-Analysis Of The Correlation Between Bispectral Index (Bis) And Clinical Sedation Scales: Toward Defining The Role Of Bis In Critically Ill Patients., Mojdeh S Heavner, Emily F Gorman, Dustin D Linn Pharm D, Siu Yan Amy Yeung, Todd A Miano

Parkview Pharmacy Department

INTRODUCTION: The bispectral index (BIS) is an attractive approach for monitoring level of consciousness in critically ill patients, particularly during paralysis, when commonly used sedation scales cannot be used.

OBJECTIVES: As a first step toward establishing the utility of BIS during paralysis, this review examines the strength of correlation between BIS and clinical sedation scales in a broad population of non-paralyzed, critically ill adults.

METHODS: We included studies evaluating the strength of correlation between concurrent assessments of BIS and Richmond Agitation Sedation Scale (RASS), Ramsay Sedation Scale (RSS), or Sedation Agitation Scale (SAS) in critically ill adult patients. Studies involving …

Lessons Learned Recruiting Comparison Elementary Schools For Impact Evaluation Of Snap-Ed Interventions, Amanda Linares, Phoebe Harpainter, Kaela Plank, Gail Woodward-Lopez

Journal of Extension

To determine the effectiveness of Supplemental Nutrition Assistance Program- Education (SNAP-Ed) nutrition and physical activity programming in elementary schools, it is necessary to recruit socioeconomically similar comparison schools not receiving SNAP-Ed programming. We developed a flexible recruitment strategy to tailor our approach to each individual school district and site. Here we discuss the lessons learned during the 10-month recruitment period, including early outreach, emphasizing participation benefits, leveraging and building relationships, and visiting sites.