Medicine and Health Sciences Commons^™

Negative Pressure Wound Therapy With Instillation: International Consensus Guidelines Update., Paul J Kim, Christopher E Attinger, Thomas Constantine, Brett D Crist, Elizabeth Faust, Christoph R Hirche, Lawrence A Lavery, Valerie J Messina, Norihiko Ohura, Laurie J Punch, Garrett A Wirth, Ibby Younis, Luc Téot

2020-Current year OA Pubs

The use of negative pressure wound therapy with instillation and dwell time (NPWTi-d) has gained wider adoption and interest due in part to the increasing complexity of wounds and patient conditions. Best practices for the use of NPWTi-d have shifted in recent years based on a growing body of evidence and expanded worldwide experience with the technology. To better guide the use of NPWTi-d with all dressing and setting configurations, as well as solutions, there is a need to publish updated international consensus guidelines, which were last produced over 6 years ago. An international, multidisciplinary expert panel of clinicians was …

Redefining And Revisiting Cost Estimates Of Routine Art Care In Zambia: An Analysis Of Ten Clinics, Austin Tucker, Tannia Tembo, Radhika P Tampi, Jacob Mutale, Mpande Mukumba-Mwenechanya, Anjali Sharma, David W Dowdy, Carolyn B Moore, Elvin Geng, Charles B Holmes, Izukanji Sikazwe, Hojoon Sohn

2020-Current year OA Pubs

INTRODUCTION: Accurate costing is key for programme planning and policy implementation. Since 2011, there have been major changes in eligibility criteria and treatment regimens with price reductions in ART drugs, programmatic changes resulting in clinical task-shifting and decentralization of ART delivery to peripheral health centres making existing evidence on ART care costs in Zambia out-of-date. As decision makers consider further changes in ART service delivery, it is important to understand the current drivers of costs for ART care. This study provides updates on costs of ART services for HIV-positive patients in Zambia.

METHODS: We evaluated costs, assessed from the health …

A Set Of Functionally-Defined Brain Regions With Improved Representation Of The Subcortex And Cerebellum, Benjamin A Seitzman, Caterina Gratton, Scott Marek, Ryan V Raut, Nico U F Dosenbach, Bradley L Schlaggar, Steven E Petersen, Deanna J Greene

2020-Current year OA Pubs

An important aspect of network-based analysis is robust node definition. This issue is critical for functional brain network analyses, as poor node choice can lead to spurious findings and misleading inferences about functional brain organization. Two sets of functional brain nodes from our group are well represented in the literature: (1) 264 volumetric regions of interest (ROIs) reported in Power et al., 2011, and (2) 333 cortical surface parcels reported in Gordon et al., 2016. However, subcortical and cerebellar structures are either incompletely captured or missing from these ROI sets. Therefore, properties of functional network organization involving the subcortex and …

Insight Gains Of Narrative Case Studies, H. Luis Vargas

Counseling and Family Therapy Scholarship Review

The Editor provides an Editorial for the new issue.

An Intervention To Improve The Evaluation Of Clerkship Students, Landon Thompson, Marc Hettlinger, Lynne J. Goebel

Marshall Journal of Medicine

Background: Effective feedback is an important part of formative evaluation of clerkship students, improving student performance by increasing awareness to weaknesses and strengths.

Aim: The aim of this study was to obtain more helpful feedback.

Setting: Internal Medicine third year clerkship rotation at Joan C Edwards School of Medicine, Huntington, WV.

Participants: The Internal Medicine department has fifty-nine general and subspecialty faculty physicians.

Program Description: We changed the structure of the existing feedback form by requesting written comments at the beginning and asking for specific strengths and areas for improvement, educated faculty, and gave them a milestones card. Three reviewers …

Modern Ventilator Management In Surgical/Trauma Patients, Errington C. Thompson

Marshall Journal of Medicine

Physicians have a lot to consider when they place a patient on the ventilator. This article reviews the literature and provides the physician with a clear guide to managing the ventilator in the ICU. This manuscript is meant to be a practical guide to managing the ventilator at the patient's bedside.

Rectal Neuroendocrine Tumor In A Patient With Chek2 Mutation, Shefali Khanna, Deena Dahshan, Erin Fankhanel, Doreen Griswold, Lynne J. Goebel

Marshall Journal of Medicine

We provide new information about CHEK2 mutation in association with a rectal neuroendocrine tumor (NET). A 67-year-old Caucasian woman diagnosed with estrogen receptor positive ductal carcinoma in situ breast cancer underwent genetic testing, revealing a CHEK2 mutation that prompted her to have a colonoscopy. This revealed a polyp with immunohistochemical staining positive for markers classically associated with NETs. Six-month follow up surveillance colonoscopy after initial polyp removal found no evidence of recurrence. While the CHEK2 mutation is known to be associated with adenocarcinomas, there is only one other reported case of its association with NETs.

Bilateral Extracranial Carotid Artery Aneurysms: A Rare Complication Of Marfan Syndrome, Lee Mendenhall, Karl G. Shaver, Lynne J. Goebel

Marshall Journal of Medicine

Marfan Syndrome (MFS) is an autosomal dominant connective tissue disorder caused by a mutation in the FBN1 gene that encodes for the extracellular matrix protein fibrillin-1. The clinical manifestations of MFS which lead to morbidity and mortality are primarily those involving aortic disease, such as aneurysmal dilation, aortic regurgitation, and aortic dissection. Despite 60-80% of adults with MFS having some degree of aortic disease, carotid artery aneurysms are rare and usually represent extensions of aortic dissections.¹ We report a 70-year-old female with a history of ectopia lentis and family history of MFS who presented to the ED with epistaxis …

Breaking The Web Of Needless Disability – Empowering The Worker, Employer, And Healthcare Provider, Mohammed Ranavaya

Marshall Journal of Medicine

No abstract provided.

The New Decade Of Scholarly Publishing, Darshana T. Shah

Marshall Journal of Medicine

No abstract provided.

Parental Deployment And Healthcare Utilization In Adolescents With Migraines And Other Somatic Symptoms, Charles Zimbrick-Rogers, Ashleigh R. Drake, Anne Peruski, Samuel Stinnette, Brian Adams

Journal of Adolescent and Family Health

Somatic symptoms in adolescents are associated with psychosocial stress. Parental military deployment can function as a stressor for adolescents. A retrospective cohort study was developed exploring relationships between health care utilization, a proxy for symptomatology, and sponsor deployment in adolescents with somatic symptoms. Data was obtained from the Military Health System Data Repository. Migraine headaches was the most common somatic symptom diagnosis. Parental deployment was overall associated with a 27% decrease in visits for somatic symptoms. For adolescents whose sponsor was female, deployment was associated with a 34% decrease in visits. This pattern is inconsistent with prior research on somatic …

Author Correction: Cancer Testis Antigen Promotes Triple Negative Breast Cancer Metastasis And Is Traceable In The Circulating Extracellular Vesicles (Scientific Reports, (2019), 9, 1, (11632), 10.1038/S41598-019-48064-W), Anbarasu Kannan, Julie V. Philley, Kate L. Hertweck, Harrison Ndetan, Karan P. Singh, Subramaniam Sivakumar, Robert B. Wells, Ratna K. Vadlamudi, Santanu Dasgupta

School of Medicine Faculty Publications and Presentations

Triple negative breast cancer (TNBC) has poor survival, exhibits rapid metastases, lacks targeted therapies and reliable prognostic markers. Here, we examined metastasis promoting role of cancer testis antigen SPANXB1 in TNBC and its utility as a therapeutic target and prognostic biomarker. Expression pattern of SPANXB1 was determined using matched primary cancer, lymph node metastatic tissues and circulating small extracellular vesicles (sEVs). cDNA microarray analysis of TNBC cells stably integrated with a metastasis suppressor SH3GL2 identified SPANXB1 as a potential target gene. TNBC cells overexpressing SH3GL2 exhibited decreased levels of both SPANXB1 mRNA and protein. Silencing of SPANXB1 reduced migration, invasion …

Covid-19 Travel Restrictions - China, January 31, 2020, University Of Northern Iowa

UNI Response to COVID-19

A memo announcing the restriction on travel to mainland China and Hong Kong.

Herpes Simplex Virus 1 Spread In Oligodendrocytic Cells Is Highly Dependent On Mal Proteolipid., José Antonio López-Guerrero, Carmen De La Nuez, Beatriz Praena, Enrique Sánchez-León, Claude Krummenacher, Raquel Bello-Morales

College of Science & Mathematics Departmental Research

Myelin and lymphocyte protein (MAL) is a tetraspan integral membrane protein that resides in detergent-insoluble membrane fractions enriched in condensed membranes. MAL is expressed in oligodendrocytes, in Schwann cells, where it is essential for the stability of myelin, and at the apical membrane of epithelial cells, where it has a critical role in transport. In T lymphocytes, MAL is found at the immunological synapse and plays a crucial role in exosome secretion. However, no involvement of MAL in viral infections has been reported so far. Here, we show that herpes simplex virus 1 (HSV-1) virions travel in association with MAL-positive …

A Review Of Teen And Adolescent Attitudes Regarding E-Cigarettes And Their Relevance To Education, Maury Grebenau

Journal of Adolescent and Family Health

Over the last ten years, while teenage use of cigarettes has diminished, their use of electronic nicotine delivery systems (e-cigarettes) has increased. Adolescent e-cigarette use includes risks and challenges which are unique and different from adolescent use of traditional cigarettes. Many of the risks of e-cigarettes are not well known or well understood, especially by the teen population. A robust understanding of what entices adolescents and teens to use e-cigarettes is critical for schools in crafting an effective response. This paper reviews the literature on teen and adolescent perspectives on e-cigarettes and provides suggestions for using this information to guide …

Human And Mouse Essentiality Screens As A Resource For Disease Gene Discovery., Pilar Cacheiro, Violeta Muñoz-Fuentes, Stephen A Murray, Mary E Dickinson, Maja Bucan, Lauryl M J Nutter, Kevin A Peterson, Hamed Haselimashhadi, Ann M Flenniken, Hugh Morgan, Henrik Westerberg, Tomasz Konopka, Chih-Wei Hsu, Audrey Christiansen, Denise G Lanza, Arthur L Beaudet, Jason D Heaney, Helmut Fuchs, Valerie Gailus-Durner, Tania Sorg, Jan Prochazka, Vendula Novosadova, Christopher J Lelliott, Hannah Wardle-Jones, Sara Wells, Lydia Teboul, Heather Cater, Michelle Stewart, Tertius Hough, Wolfgang Wurst, Radislav Sedlacek, David J Adams, John R Seavitt, Glauco Tocchini-Valentini, Fabio Mammano, Robert E Braun, Colin Mckerlie, Yann Herault, Martin Hrabě De Angelis, Ann-Marie Mallon, K C Kent Lloyd, Steve D M Brown, Helen Parkinson, Terrence F Meehan, Damian Smedley, Genomics England Research Consortium, International Mouse Phenotyping Consortium

Faculty Research 2020

The identification of causal variants in sequencing studies remains a considerable challenge that can be partially addressed by new gene-specific knowledge. Here, we integrate measures of how essential a gene is to supporting life, as inferred from viability and phenotyping screens performed on knockout mice by the International Mouse Phenotyping Consortium and essentiality screens carried out on human cell lines. We propose a cross-species gene classification across the Full Spectrum of Intolerance to Loss-of-function (FUSIL) and demonstrate that genes in five mutually exclusive FUSIL categories have differing biological properties. Most notably, Mendelian disease genes, particularly those associated with developmental disorders, …

Demarcation Of Sepsis-Induced Peripheral And Central Acidosis With Ph (Low) Insertion Cycle Peptide, Kelly E. Henry, Aisling M. Chaney, Veronica L. Nagle, Haley C. Cropper, Saghar Mozaffari, Chip Slaybaugh, Keykavous Parang, Oleg A. Andreev, Yana K. Reshetnyak, Michelle L. James, Jason S. Lewis

Pharmacy Faculty Articles and Research

Acidosis is a key driver for many diseases, including cancer, sepsis, and stroke. The spatiotemporal dynamics of dysregulated pH across disease remain elusive, and current diagnostic strategies do not provide localization of pH alterations. We sought to explore if PET imaging using hydrophobic cyclic peptides that partition into the cellular membrane at low extracellular pH (denoted as pH [low] insertion cycles, or pHLIC) can permit accurate in vivo visualization of acidosis. Methods: Acid-sensitive cyclic peptide c[E₄W₅C] pHLIC was conjugated to bifunctional maleimide-NO2A and radiolabeled with ⁶⁴Cu (half-life, 12.7 h). C57BL/6J mice were administered lipopolysaccharide (15 …

Solving The Sepsis Puzzle: Every Minute Counts, Jamie Roney, Amber Cline

Articles, Abstracts, and Reports

SPEMS Pre-Conference Course Sepsis Class

Angioimmunoblastic T Cell Lymphoma: A Diagnostic Dilemma, Nishanth Thalambedu, Md, Seema Sethi, Do, Herbert Auerbach, Do

Abington Jefferson Health Posters

Angioimmunoblastic T cell lymphoma(AITL) is one of the rarest T cell lymphomas. Diagnosing the type of lymphoma is crucial, as the management differs depending on the type. We present a case of a sixty-one-year-old woman who presented with generalized lymphadenopathy and how we unfolded the diagnosis.

College Of Public Health News, Georgia Southern University

College of Public Health: News & Publications

• Georgia Southern associate dean named to National Advisory Mental Health Council
• Georgia Southern to offer new online Master of Public Health in Fall 2020
• Jiann-Ping Hsu College of Public Health Eta Sigma Gamma chapter receives national award

Evaluation Of The Effect Of Gm-Csf Blocking On The Phenotype And Function Of Human Monocytes., Noushin Lotfi, Guang-Xian Zhang, Nafiseh Esmaeil, Mohamad Rostami

Department of Neurology Faculty Papers

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a multipotent cytokine that prompts the proliferation of bone marrow-derived macrophages and granulocytes. In addition to its effects as a growth factor, GM-CSF plays an important role in chronic inflammatory autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. Reports have identified monocytes as the primary target of GM-CSF; however, its effect on monocyte activation has been under-estimated. Here, using flow cytometry and ELISA we show that GM-CSF induces an inflammatory profile in human monocytes, which includes an upregulated expression of HLA-DR and CD86 molecules and increased production of TNF-α and IL-1β. Conversely, blockage of …

Identification Of Functional Regulatory Elements In The Human Genome Using Pooled Crispr Screens., Samantha M. Borys, Scott T. Younger

Manuscripts, Articles, Book Chapters and Other Papers

BACKGROUND: Genome-scale pooled CRISPR screens are powerful tools for identifying genetic dependencies across varied cellular processes. The vast majority of CRISPR screens reported to date have focused exclusively on the perturbation of protein-coding gene function. However, protein-coding genes comprise < 2% of the sequence space in the human genome leaving a substantial portion of the genome uninterrogated. Noncoding regions of the genome harbor important regulatory elements (e.g. promoters, enhancers, silencers) that influence cellular processes but high-throughput methods for evaluating their essentiality have yet to be established.

RESULTS: Here, we describe a CRISPR-based screening approach that facilitates the functional profiling of thousands of noncoding regulatory elements in parallel. We selected the tumor suppressor p53 as a model system and designed a pooled CRISPR library targeting thousands of p53 binding sites throughout the genome. Following transduction into dCas9-KRAB-expressing cells we identified several regulatory elements that influence cell proliferation. Moreover, …

Evaluation Of The Effect Of Gm-Csf Blocking On The Phenotype And Function Of Human Monocytes, Noushin Lotfi, Guang-Xian Zhang, Nafiseh Esmaeil, Mohamad Rostami

Department of Neurology Faculty Papers

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a multipotent cytokine that prompts the proliferation of bone marrow-derived macrophages and granulocytes. In addition to its effects as a growth factor, GM-CSF plays an important role in chronic inflammatory autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. Reports have identified monocytes as the primary target of GM-CSF; however, its effect on monocyte activation has been under-estimated. Here, using flow cytometry and ELISA we show that GM-CSF induces an inflammatory profile in human monocytes, which includes an upregulated expression of HLA-DR and CD86 molecules and increased production of TNF-α and IL-1β. Conversely, blockage of …

People With Parkinson’S And Care Partners Of Pwps’ Uncertainty Management Through Information Strategies, Amy Haywood

USF Tampa Graduate Theses and Dissertations

This thesis explores how people with Parkinson’s (PwP) and care partners of PwP manage the uncertainty surrounding Parkinson’s disease (PD). PD causes both motor and cognitive decline in patients, which may affect each individual differently (Williamson, Simpson, & Murray, 2008). There is no-known cure or cause for PD, meaning it is shrouded in uncertainty from the first symptom to the ongoing management process. This disease also affects care partners because of its variability in symptoms and progression. For this reason, this research, which is part of a larger study, interviewed both care partners of PwP (n= 10) and those diagnosed …

Treatments For Cancer Given Orally: Patients' Perceptions Of Distress Due To Financial Toxicity, Ellen Carr

Dissertations

Purpose/Aims: For adult participants who have received or are receiving treatment for hematologic and solid tumor malignancies given orally, this study describes the relationship between participants’ experience of financial toxicity (FT), the participants’ perception of distress associated with FT, and participants’ self-identified adherence to prescribed treatments in the context of FT.

Background: FT has emerged as an additional source of distress for cancer patients. The costs of treatments given orally can be prohibitively expensive for patients. Therefore, these patients may experience considerable distress and may not adhere to treatments as prescribed.

Method: Descriptive cross-sectional correlational design study of a sample …

A Transcriptome-Wide Association Study Based On 27 Tissues Identifies 106 Genes Potentially Relevant For Disease Pathology In Age-Related Macular Degeneration, Tobias Strunz, Susette Lauwen, Christina Kiel, Lars G. Fritsche, Wilmar Igl, Jessica N.Cooke Bailey, Felix Grassmann, Sebanti Sengupta, Jennifer L. Bragg-Gresham, Matthew P. Johnson, John Blangero

School of Medicine Publications

Genome-wide association studies (GWAS) for late stage age-related macular degeneration (AMD) have identified 52 independent genetic variants with genome-wide significance at 34 genomic loci. Typically, such an approach rarely results in the identification of functional variants implicating a defined gene in the disease process. We now performed a transcriptome-wide association study (TWAS) allowing the prediction of effects of AMD-associated genetic variants on gene expression. The TWAS was based on the genotypes of 16,144 late-stage AMD cases and 17,832 healthy controls, and gene expression was imputed for 27 different human tissues which were obtained from 134 to 421 individuals. A linear …

Assessing And Reducing The Elusive Faculty Workload: The Role Of The Graduate Nursing Student Faculty Navigator, Jane Garvin, Amy Herrington

Nursing Collection

Nursing programs have struggled with defining the faculty workload. Most academic institutions with a healthcare focus use a percentage of time allotted for teaching, research, service, and practice. The mission of the institution often dictates which of the four carries the most weight. Some institutions define the teaching load based on academic hours while others use contact hours. Typically, service receives the smallest percentage of the assigned faculty workload. Yet, service is the area in which many miscellaneous tasks are allocated.

Student advising, committee service, and service to the profession are often incorporated in the small percentage of time allotted …

Health Care Use And Access Among Rural And Urban Nonelderly Adult Medicare Beneficiaries, Erika C. Ziller Phd, Amanda Burgess Mppm, Deborah Thayer Mba

Access / Insurance

Little is known about the characteristics and health care use of rural residents with disabilities. Using the Medicare Current Beneficiary Survey (2009-2013), we compared access to and use of health services among rural and urban nonelderly Medicare beneficiaries with a disability, and examined their health and functional status along with sociodemographic characteristics. We found that the characteristics of nonelderly Medicare beneficiaries with a disability reflected the differences observed between rural and urban populations overall: rural recipients were more likely than their urban peers to be older, non-Hispanic white, and have a lower level of educational attainment. Although self-reported access to …

Potential Use Of Biotherapeutic Bacteria To Target Colorectal Cancer-Associated Taxa, Gareth Lawrence, Maire Begley, Paul D. Cotter, Caitríona M. Guinane

Department of Biological Sciences Publications

The role of the gut microbiome in human health and disease is the focus of much attention. It has been widely agreed upon that our gut bacteria play a role in host immunity, nutrient absorption, digestion, metabolism, and other key drivers of health. Furthermore, certain microbial signatures and specific taxa have also been associated with the development of diseases, such as obesity; inflammatory bowel disease; and, indeed, colorectal cancer (CRC), which is the focus of this review. By extension, such taxa represent potential therapeutic targets. In particular, the emerging human pathogen Fusobacterium nucleatum represents an important agent in CRC development …

The Relationship Between Grit And Selected Personality Measures In Medical Students, Gerald A. Isenberg, Andrew Brown, Jennifer Desantis, Jon Veloski, Mohammadreza Hojat

CRMEHC Faculty Papers

OBJECTIVES: To test the hypothesis that scores on a Grit scale are positively associated with personality measures that are conducive to relationship building (Empathy, Self-Esteem, Activity, and Sociability), but inversely associated with personality measures that are detrimental to interpersonal relationships (Neuroticism-Anxiety, Aggression-Hostility, Impulsive Sensation Seeking, and Loneliness).

METHODS: Convenient sampling was used that included 241 medical students at Sidney Kimmel Medical College at Thomas Jefferson University who participated in this ex post facto research. Validated instruments were used to measure Grit, Empathy, Self-Esteem, Activity, Sociability, Neuroticism-Anxiety, Aggression-Hostility, Impulsive Sensation Seeking, and Loneliness. Bivariate correlations and multivariate regression were used to …