Medicine and Health Sciences Commons^™

Comparison Of The Sensitivity Of Biomarker Based Matrix Assisted Laser Desorption Ionization-Time Of Flight Mass Spectrometry (Maldi-Tof Ms) And Conventional Methods In The Identification Of Clinically Relevant Bacteria And Yeast, Ali Abdow Kassim

Master of Medicine in Clinical Pathology

Background: MALDI-TOF MS is an analytical method that has recently become integral in the identification of microorganisms in clinical laboratories. It relies on databases that majorly employ pattern recognition or fingerprinting. Biomarker based databases have also been developed and there is optimism that these may be superior to pattern recognition based databases. This study compared the performance of ribosomal biomarker based MALDI-TOF MS and conventional methods in the identification of selected bacteria and yeast. Methodology: The study was a cross sectional study carried out in collaboration with the Swiss Tropical and Public Health Institute, Switzerland and Mabritec laboratory, Riehen, Switzerland. …

Frameless Image-Guided Radiosurgery For Trigeminal Neuralgia, Lisa B. E. Shields, Todd S. Shanks, Andrew J. Shearer, Lauren A. Shelton, Brent J. Shelton, Jonathan Howe, James M. Coons, Brian Plato, Aaron C. Spalding

Markey Cancer Center Faculty Publications

Background: Frameless image-guided radiosurgery (IGRS) is a safe and effective noninvasive treatment for trigeminal neuralgia (TN). This study evaluates the use of frameless IGRS to treat patients with refractory TN.

Methods: We reviewed the records of 20 patients diagnosed with TN who underwent frameless IGRS treatments between March 2012 and December 2013. Facial pain was graded using the Barrow Neurological Institute (BNI) scoring system. The initial setup uncertainty from simulation to treatment and the patient intrafraction uncertainty were measured. The median follow-up was 32 months.

Results: All patients' pain was BNI Grade IV or V before the frameless IGRS treatment. …

Response To “Cortisol In Human Milk: The Good, The Bad, Or The Ugly?”, Jennifer Hahn-Holbrook, Elysia P. Davis, Laura M. Glynn

Psychology Faculty Articles and Research

A response to Finken et al.'s "Cortisol in Human Milk: The Good, the Bad, or the Ugly?", a critique of the authors' previous article "Cortisol in human milk predicts child BMI".

Inside Out, Spring 2017

Inside Out

Contents:

Abstract Anatomy: The Strength Within, Jordyn Brooke Tumas, Front Cover

Lower East Side, Caroline Christianson, Inside Cover

Untitled, Alex Rowan, 1

Daytime Figure Study, Chen Zhou, 2

Amyloids, Malika Madhava, 3

Dirty Dishes, Daisy Zhang, 4

Topography, Prachi Priyam, 5

Glass Wall, South Street, Sanket Patkak, 5

Our home is open, Julie Christensen, 6

Hands, Katie Shen, 7

Balance, Anna Melnick, 8

Consequence, Anitha Ahmed, 9

Woman with White Carnation, Katie Sommers, 10

pocketbooks, Anthony K. Vu, 10

Miniatures, Holly Rankin, 11

Savory Poem, Anya Platt, 11

Rememberance, Anya Platt, 12

Paper Doll, Daisy Zhang, 13

Miniatures, Holly Rankin …

Inside Out, Spring 2016

Inside Out

Contents:

Untitled, Amanda Breem, Front Cover

Stockholm Underground, David Chou, Inside Front Cover

Portrait of a Woman, Chen Zhou, Opposite Page

The Happy Prince, Yuhang Zhou, 1

Feeling Limited, Amy Tressan, 2

Winged Scapula, Daisy Zhang, 3

Untitled, Kathryn Tuazon, 4

Cooking with Papa in Ramadan, Anitha Ahmed, 6

Not Sure Where I'm Going, Jessica Gatt, 6

Salt Marsh, Jessica Gatt, 7

Bite your liquor, not your tongue, Daisy Zhang, 8

Dr. Frank N. Furter, Kathryn Tuazon, 9

Calm, Jessica Gatt, 10

River Basin, Jessica Gatt, 11

Thoracic Vertebra, Holly Rankin, 12

Scapula 2, Holly Rankin, 13

The Kacha Bazaar, …

A Case Of Transient St Elevation And Polymorphic Tachycardia Without Angina Diagnosed By Holter Monitoring, Ali Farooq, Fahad Alqahtani, Almoutassim Trabulsi, Akram Kawsara, Mohamad Alkhouli

Clinical and Translational Science Institute

We report the case of 52-year-old female with recurrent episodes of palpitations and dizziness. Holter monitoring revealed transient ST elevations followed by episodes of polymorphic ventricular tachycardia associated with episodes of palpitations and dizziness. Coronary angiography revealed mildly irregular right coronary artery with 90% stenosis. The patient underwent percutaneous coronary intervention with successful placement of a stent to the mid-right coronary artery. The patient has been followed closely over a period of 12 months. There haven't been any recorded episodes of tachycardia, and the patient has remained symptom-free.

Private Sector Role, Readiness And Performance For Malaria Case Management In Uganda, 2015., Kathryn A. O'Connell, Vamsi Vasireddy, Megan Littrell, Andria Rusk, Actwatch Group, Henry Kaula, Peter Buyungo, Jimmy Opigo

Collected Faculty Scholarship

BACKGROUND: Several interventions have been put in place to promote access to quality malaria case management services in Uganda's private sector, where most people seek treatment. This paper describes evidence using a mixed-method approach to examine the role, readiness and performance of private providers at a national level in Uganda. These data will be useful to inform strategies and policies for improving malaria case management in the private sector.

METHODS: The ACTwatch national anti-malarial outlet survey was conducted concurrently with a fever case management study. The ACTwatch nationally representative anti-malarial outlet survey was conducted in Uganda between May 18th 2015 …

Do Anti-Malarials In Africa Meet Quality Standards? The Market Penetration Of Non Quality-Assured Artemisinin Combination Therapy In Eight African Countries., Kathryn A. O'Connell, Vamsi Vasireddy, Megan Littrell, Andria Rusk, Actwatch Group, Paul N Newton, Kara Hanson, Catherine Goodman

Collected Faculty Scholarship

BACKGROUND: Quality of artemisinin-based combination therapy (ACT) is important for ensuring malaria parasite clearance and protecting the efficacy of artemisinin-based therapies. The extent to which non quality-assured ACT (non-QAACT), or those not granted global regulatory approval, are available and used to treat malaria in endemic countries is poorly documented. This paper uses national and sub-national medicine outlet surveys conducted in eight study countries (Benin, Kinshasa and Kantanga [Democratic Republic of the Congo, DRC], Kenya, Madagascar, Nigeria, Tanzania, Uganda and Zambia) between 2009 and 2015 to describe the non-QAACT market and to document trends in availability and distribution of non-QAACT in …

Synchronized And Mixed Outbreaks Of Coupled Recurrent Epidemics, Muhua Zheng, Ming Zhao, Byungjoon Min, Zonghua Liu

Publications and Research

Epidemic spreading has been studied for a long time and most of them are focused on the growing aspect of a single epidemic outbreak. Recently, we extended the study to the case of recurrent epidemics (Sci. Rep. 5, 16010 (2015)) but limited only to a single network. We here report from the real data of coupled regions or cities that the recurrent epidemics in two coupled networks are closely related to each other and can show either synchronized outbreak pattern where outbreaks occur simultaneously in both networks or mixed outbreak pattern where outbreaks occur in one network but do not …

Who Helps Our Helpers?: Poster At Scott Library, Daniel G. Kipnis, Msi

Jefferson Digital Commons News

Visit the 2nd floor gallery area of Scott Library this summer to view a 9-foot poster entitled Who Helps Our Helpers? Rediscovering Joy in Medicine by Addressing Secondary

Disulfide High Mobility Group Box-1 Causes Bladder Pain Through Bladder Toll-Like Receptor 4, Fei Ma, Dimitrios E. Kouzoukas, Katherine L. Meyer-Siegler, Karin N. Westlund, David E. Hunt, Pedro L. Vera

Physiology Faculty Publications

Background: Bladder pain is a prominent symptom in several urological conditions (e.g. infection, painful bladder syndrome/interstitial cystitis, cancer). Understanding the mechanism of bladder pain is important, particularly when the pain is not accompanied by bladder pathology. Stimulation of protease activated receptor 4 (PAR4) in the urothelium results in bladder pain through release of urothelial high mobility group box-1 (HMGB1). HGMB1 has two functionally active redox states (disulfide and all-thiol) and it is not known which form elicits bladder pain. Therefore, we investigated whether intravesical administration of specific HMGB1 redox forms caused abdominal mechanical hypersensitivity, micturition changes, and bladder inflammation in …

Effects Of Winning Percentage On Men’S Collegiate Basketball After Investments In Facilities, Matthew D. Frank

Purdue Polytechnic Directed Projects

Throughout studying the impacts of investments on facilities and its potential impacts on Men’s Basketball overall success within the conferences we were able to find some indicators that would suggest the financial investments do have an impact but we would need to study more teams to see a possible statistical significance. Although statistical significance was not found within the current study (.13 was found with an alpha set at .10) my suggestion and observations throughout this study show that facilities play a major role in the overall success of a program and spending will continue to take place as the …

Comparison Of Biomarker Based Matrix Assisted Laser Desorption Ionization-Time Of Flight Mass Spectrometry (Maldi-Tof Ms) And Conventional Methods In The Identification Of Clinically Relevant Bacteria And Yeast, Ali Kassim, Valentin Pflüger, Zul Premji, Claudia Daubenberger, Gunturu Revathi

Pathology, East Africa

Background: MALDI-TOF MS is an analytical method that has recently become integral in the identification of microorganisms in clinical laboratories. It relies on databases that majorly employ pattern recognition or fingerprinting. Biomarker based databases have also been developed and there is optimism that these may be superior to pattern recognition based databases. This study compared the performance of ribosomal biomarker based MALDI-TOF MS and conventional methods in the identification of selected bacteria and yeast.

Methods: The study was a cross sectional study identifying clinically relevant bacteria and yeast isolated from varied clinical specimens submitted to a clinical laboratory. The identification …

Development And Initial Assessment Of A Patient Education Video About Pharmacogenetics., Rachel Mills, Megan Ensinger, Nancy Callanan, Susanne B Haga

Medical Education Articles

As few patient-friendly resources about pharmacogenetics are currently available, we aimed to create and assess a patient educational video on pharmacogenetic testing. A primary literature and resources review was conducted to inform the content and the format of the video. The educational video was then created using a commercially available animation program and pilot tested in focus groups of the general public and by an online survey of pharmacists. Emerging themes from the focus groups and survey indicate a desire for appropriate risk contextualization and specific examples when pharmacogenetic testing may be beneficial. Focus group participants also expressed a preference …

Intercepting The Intergenerational Trauma Of Mass Incarceration Through Art-Based Parent Programs, Ana Ruth Yela Castillo

LMU Theses and Dissertations

This study discusses the intergenerational impact of mass incarceration on families. The general literature repeatedly described the negative effects of mass incarceration among children who have an incarcerated parent by pointing to the difficulty of educational attainment, social exclusion, stigma, substance abuse, and the exacerbation of mental health issues such as depression, anxiety, and antisocial behavior (Kjellstrand & Eddy, 2011; Miller & Barnes, 2015; Turney, 2014). Unfortunately, most incarcerated individuals are parents and most incarcerated women are mothers (Scudder, A., et al., 2014, and Miller, et al., 2014). Through the use of art, service providers (artists, clinicians, etc.) that facilitate …

Nano-Assembly Of Amyloid Β Peptide: Role Of The Hairpin Fold., Sibaprasad Maity, Mohtadin Hashemi, Yuri L. Lyubchenko

Journal Articles: Pharmaceutical Sciences

Structural investigations have revealed that β hairpin structures are common features in amyloid fibrils, suggesting that these motifs play an important role in amyloid assembly. To test this hypothesis, we characterized the effect of the hairpin fold on the aggregation process using a model β hairpin structure, consisting of two Aβ(14-23) monomers connected by a turn forming YNGK peptide. AFM studies of the assembled aggregates revealed that the hairpin forms spherical structures whereas linear Aβ(14-23) monomers form fibrils. Additionally, an equimolar mixture of the monomer and the hairpin assembles into non-fibrillar aggregates, demonstrating that the hairpin fold dramatically changes the …

Paranormal Activity: Para-Esophageal/Gastric Hemorrhage In A Patient With A Heartmate Ii Lvad, Anna Stone, Ashwin Ravichandran

The VAD Journal

In this report we present an unusual case of non-traumatic, spontaneous para-esophageal/para-gastric hemorrhage requiring multiple units of red blood cells in a female patient with a continuous flow left ventricular assist device. She presented with dysphagia and atypical chest pain 18 months post implantation, on the recommended anticoagulation regimen for HeartMate II support of ASA 81mg and international normalized ratio goal (INR) of 2-3 and was successfully managed with conservation support, volume resuscitation and multiple transfusions. Non-traumatic causes of acute bleeding, including acquired platelet dysfunction are considered.

A Cell-Surface Membrane Protein Signature For Glioblastoma., Dhimankrishna Ghosh, Cory C Funk, Juan Caballero, Nameeta Shah, Katherine Rouleau, John C Earls, Liliana Soroceanu, Greg Foltz, Charles Cobbs, Nathan D Price, Leroy Hood

Articles, Abstracts, and Reports

We present a systems strategy that facilitated the development of a molecular signature for glioblastoma (GBM), composed of 33 cell-surface transmembrane proteins. This molecular signature, GBMSig, was developed through the integration of cell-surface proteomics and transcriptomics from patient tumors in the REMBRANDT (n = 228) and TCGA datasets (n = 547) and can separate GBM patients from control individuals with a Matthew's correlation coefficient value of 0.87 in a lock-down test. Functionally, 17/33 GBMSig proteins are associated with transforming growth factor β signaling pathways, including CD47, SLC16A1, HMOX1, and MRC2. Knockdown of these genes impaired GBM invasion, reflecting their role …

Offering Self-Administered Oral Hiv Testing As A Choice To Truck Drivers In Kenya: Predictors Of Uptake And Need For Guidance While Self-Testing, Elizabeth A. Kelvin, Gavin George, Eva Mwai, Eston N. Nyaga, Joanne E. Mantell, Matthew L. Romo, Jacob O. Odhiambo, Kaymarlin Govender

Publications and Research

We assessed predictors of choosing self-administered oral HIV testing in the clinic with supervision versus the standard provider-administered blood test when offered the choice among 149 Kenyan truck drivers, described the types of guidance participants needed during self-testing and predictors of needing guidance. Overall, 56.38% of participants chose the self-test, 23.49% the provider-administered test, and 20.13% refused testing. In the adjusted regression models, each additional unit on the fatalism and self-efficacy scales was associated with 0.97 (p = 0.003) and 0.83 (p = 0.008) times lower odds of choosing the self-test, respectively. Overall, 52.38% of self-testers did so correctly without …

Foundational Knowledge And Other Predictors Of Commitment To Trauma-Informed Care, Stephanie Anne Sundborg

Dissertations and Theses

Trauma-informed care (TIC) is an approach to service delivery based on the understanding of the prevalence of psychological trauma among service users, knowledge about the impact trauma has on engagement to services, and recognition that service settings can be re-traumatizing. For more than a decade, momentum has been building on this topic. Practitioners are pursuing the knowledge and skills needed to implement trauma-informed service delivery, while organizations are building infrastructure and processes aimed at supporting this approach. Disciplines across many human service sectors are eager to incorporate TIC into policy and practice. Despite this enthusiasm, implementation efforts are slow. Acquiring …

N-Blr, A Primate-Specific Non-Coding Transcript Leads To Colorectal Cancer Invasion And Migration., Isidore Rigoutsos, Sang Kil Lee, Su Youn Nam, Simone Anfossi, Barbara Pasculli, Martin Pichler, Yi Jing, Cristian Rodriguez-Aguayo, Aristeidis G. Telonis, Simona Rossi, Cristina Ivan, Tina Catela Ivkovic, Linda Fabris, Peter M. Clark, Hui Ling, Masayoshi Shimizu, Roxana S. Redis, Maitri Y Shah, Xinna Zhang, Yoshinaga Okugawa, Eun Jung Jung, Aristotelis Tsirigos, Li Huang, Jana Ferdin, Roberta Gafà, Riccardo Spizzo, Milena S. Nicoloso, Anurag N. Paranjape, Maryam Shariati, Aida Tiron, Jen Jen Yeh, Raul Teruel-Montoya, Lianchun Xiao, Sonia A. Melo, David Menter, Zhi-Qin Jiang, Elsa R. Flores, Massimo Negrini, Ajay Goel, Menashe Bar-Eli, Sendurai A. Mani, Chang Gong Liu, Gabriel Lopez-Berestein, Ioana Berindan-Neagoe, Manel Esteller, Scott Kopetz, Giovanni Lanza, George A. Calin

Computational Medicine Center Faculty Papers

BACKGROUND: Non-coding RNAs have been drawing increasing attention in recent years as functional data suggest that they play important roles in key cellular processes. N-BLR is a primate-specific long non-coding RNA that modulates the epithelial-to-mesenchymal transition, facilitates cell migration, and increases colorectal cancer invasion.

RESULTS: We performed multivariate analyses of data from two independent cohorts of colorectal cancer patients and show that the abundance of N-BLR is associated with tumor stage, invasion potential, and overall patient survival. Through in vitro and in vivo experiments we found that N-BLR facilitates migration primarily via crosstalk with E-cadherin and ZEB1. We showed that …

Using Quicksin Speech Material To Measure Acceptable Noise Level For Adults With Hearing Loss, Lauren E. Bentley, Hua Ou

AuD Capstone Projects - Communication Sciences and Disorders

It is clinically useful to predict potential for hearing aid use success prior to the initial fitting of hearing aids. This information may be used to help with additional counseling for speech understanding in noisy listening environments and can also be used to describe the use of communication skills in combination with the dynamic functionality of hearing aids to guide patients that are hearing aid candidates towards success. The Quick Speech-In-Noise (QuickSIN) test and the Acceptable Noise Level (ANL) test have been recognized for their ability to predict hearing aid use success; however, the two measures use different stimuli and …

The 11s Proteasomal Activator Regγ Impacts Polyglutamine-Expanded Androgen Receptor Aggregation And Motor Neuron Viability Through Distinct Mechanisms., Jill M. Yersak, Heather L. Montie, Erica S. Chevalier-Larsen, Yuhong Liu, Lan Huang, Martin Rechsteiner, Diane E. Merry

Department of Biochemistry and Molecular Biology Faculty Papers

Spinal and bulbar muscular atrophy (SBMA) is caused by expression of a polyglutamine (polyQ)-expanded androgen receptor (AR). The inefficient nuclear proteasomal degradation of the mutant AR results in the formation of nuclear inclusions containing amino-terminal fragments of the mutant AR. PA28γ (also referred to as REGγ) is a nuclear 11S-proteasomal activator with limited proteasome activation capabilities compared to its cytoplasmic 11S (PA28α, PA28β) counterparts. To clarify the role of REGγ in polyQ-expanded AR metabolism, we carried out genetic and biochemical studies in cell models of SBMA. Overexpression of REGγ in a PC12 cell model of SBMA increased polyQ-expanded AR aggregation …

Mixed Method Study Of Condom Use Among Emerging Adults With New Sex Partners Met Online Or Offline, Shana M. Green

USF Tampa Graduate Theses and Dissertations

Introduction: Emerging adults (young people age 18 to 29) are increasingly using the Internet to seek sex partners, with over 30% having tried an online or mobile dating site. As more emerging adults use dating and sex-seeking websites (DSP) for their love and sexual pursuits, it is imperative to understand how DSPs contribute to HIV/STI risk.

Objectives: The objectives of this research were accomplished by pursuing the following specific aims: Aim 1. To systematically review and quantitatively synthesize evidence from published findings to determine the association between condom use and meeting venue (online and offline). Aim 2. To determine whether …

Binding Of Cib1 To The Αiib Tail Of Αiibβ3 Is Required For Fak Recruitment And Activation In Platelets., Meghna U. Naik, Tejal U. Naik, Ross Summer, Ulhas P. Naik

Department of Medicine Faculty Papers

BACKGROUND: It is believed that activation of c-Src bound to the integrin β3 subunit initiates outside-in signaling. The involvement of αIIb in outside-in signaling is poorly understood.

OBJECTIVES: We have previously shown that CIB1 specifically interacts with the cytoplasmic domain of αIIb and is required for αIIbβ3 outside-in signaling. Here we evaluated the role of CIB1 in regulating outside-in signaling in the absence of inside-out signaling.

METHODS: We used αIIb cytoplasmic domain peptide and CIB1-function blocking antibody to inhibit interaction of CIB1 with αIIb subunit as well as Cib1-/- platelets to evaluate the consequence of CIB1 interaction with αIIb on …

Endocrine And Metabolic Effects Of Consuming Sugar-Sweetened Beverages: Preclinical And Clinical Studies", Peter Havel

Science Seminar Series

Peter J. Havel of the School of Veterinary Medicine at UC Davis will speak on his research on Endocrine and Metabolic Effects of Consuming Sugar-Sweetened Beverages: Preclinical and clinical studies at this Science Seminar Series lecture.

Public Schools, And Health Care: A Strategy To Promote Social Inclusion, Jana Sladkova, Anahi Viladrich, Nicholas Freudenberg

Occasional Paper Series

Sladkova, Viladrich, and Freudenberg refer to “social inclusion” as the process through which the newly arrived find their voice in an already complex, cacophonous society. They describe an approach to social inclusion for adult immigrants that melds learning English at the same time as learning to negotiate our often-Byzantine health care system. They highlight programs that work and a new perspective on how to maximize the effectiveness of limited adult education opportunities.

The Effects Of The Incentive Approach On Physical Activity And Sleep Utilizing Wearable Fitness Technology, Katie A. Schmidt, Courtney J. Sheets, Kassi A. Thiel, Morgan G. Wolf, Seth A. Paradis

Applied Health Sciences Student Works

PURPOSE: Wearable fitness technology (WFT) is a relevant tool in analyzing physical activity and sleep. Sleep and physical activity have symbiotic roles in maintaining wellness and returning the body to homeostasis. Currently, research is lacking in demonstrating effects of intensification on physical activity using WFT. This study analyzed the effects of incentivisation on physical activity and its relationship to sleep using the UP Jawbone. METHODS: 35 Bethel University students (16 males, 19 females) were recruited, ages 18-23 (20.49 years ± 1.44 years) and randomized into the control (17) or incentivized group (18). Sleep and physical activity data were recorded for …

Modulation Of Estrogen Related Receptor Alpha Activity By The Kinesin Kif17, Am Pramodh Bandara Seneviratne, Zeynep Turan, Aurelie Hermant, Patrick Lecine, William O. Smith, Jean-Paul Borg, Fanny Jaulin, Geri Kreitzer

Publications and Research

Estrogen-related receptor alpha (ERR1) is an orphan nuclear receptor that can bind transcriptional co-activators constitutively. ERR1 expression correlates with poor patient outcomes in breast cancer, heightening interest in this nuclear receptor as a therapeutic target. Because ERR1 has no known regulatory ligand, a major challenge in targeting its activity is to find cellular or synthetic modulators of its function. We identified an interaction between ERR1 and KIF17, a kinesin-2 family microtubule motor, in a yeast-2-hybrid screen. We confirmed the interaction using in vitro biochemical assays and determined that binding is mediated by the ERR1 ligand-binding/AF2 domain and the KIF17 C-terminal …

Coming To The New D.A.R.E.: A Preliminary Test Of The Officer-Taught Elementary Keepin’ It Real Curriculum, L. Edward Day, Michelle Miller-Day, Michael L. Hecht, Desiree Fehmie

Communication Faculty Articles and Research

The present study reports a preliminary evaluation of D.A.R.E.’s new elementary school keepin’ it REAL substance abuse prevention program. Given the widespread dissemination of D.A.R.E., this evaluation, even though of short term effects, has important implications for national prevention efforts. The new prevention curriculum teaches social and emotional competencies such as decision making and resistance skills. Social and emotional competencies and other risk factors were examined among students (N = 943) in 26 classrooms, 13 classrooms in the treatment condition (n = 359) and 13 classrooms in the control condition (n = 584) using a quasi-experimental matched group design. Pretest …