Medicine and Health Sciences Commons^™

Defining Speech And Language Fluency Profiles Before And After The Onset Of Stuttering: Preliminary Findings, Charn Nang

ECU Research Week

No abstract provided.

Association Of Maximum Weight With Hyperuricemia Risk: A Retrospective Study Of 21,414 Chinese People., Bin Gao, Jie Zhou, Jiapu Ge, Yaping Zhang, Fei Chen, Wayne B Lau, Yi Wan, Nanyan Zhang, Ying Xing, Li Wang, Jianfang Fu, Xiaomiao Li, Hongxia Jia, Xin Zhao, Qiuhe Ji

Department of Emergency Medicine Faculty Papers

BACKGROUND: Obesity has been demonstrated to be associated with increased serum uric acid (SUA); however, little is known regarding the relationship between maximum weight, or maximum weight fluctuation, and uric acid concentration. Through retrospective means, we determined the association of maximum weight with SUA risk.

METHODS: Data of 21,414 participants (8,630 males and 12,784 females) from the 2007-8 China National Diabetes and Metabolic Disorders Study were analyzed for parameters including lifestyle habits, biochemical blood analysis and self-reported maximum weight.

RESULTS: Elevated SUA subjects shared a cluster of demographic features. After adjustment for age, gender, education, smoking, drinking, physical activity, WHR, …

Conversion From Enzyme-Inducing Antiepileptic Drugs To Topiramate: Effects On Lipids And C-Reactive Protein., Scott Mintzer, Christopher T Skidmore, Sara J Rankin, Inna Chervoneva, Edward Pequinot, David M Capuzzi, Michael R Sperling

Department of Neurology Faculty Papers

PURPOSE: We previously demonstrated that converting patients from the enzyme-inducers phenytoin or carbamazepine to the non-inducers levetiracetam or lamotrigine reduces serum lipids and C-reactive protein (CRP). We sought to determine if the same changes would occur when patients were switched to topiramate, which has shown some evidence of enzyme induction at high doses. We also examined the effects of drug switch on low-density lipoprotein (LDL) particle concentration.

METHODS: We converted 13 patients from phenytoin or carbamazepine monotherapy to topiramate monotherapy (most at doses of 100-150 mg/day). Fasting lipids, including LDL particle concentration, and CRP were obtained before and ≥6 weeks …

Mir-145 Directly Targets P70s6k1 In Cancer Cells To Inhibit Tumor Growth And Angiogenesis., Qing Xu, Ling-Zhi Liu, Xu Qian, Qi Chen, Yue Jiang, Dan Li, Lihui Lai, Bing-Hua Jiang

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

MiR-145 can regulate cell apoptosis, proliferation, neural development and stem cell differentiation. Previous studies indicate that miR-145 is downregulated in human colon cancer cells. However, the molecular mechanisms of miR-145 used to regulate colon carcinogenesis and angiogenesis remain to be clarified. Here, we show that the expression of miR-145 is downregulated in colon and ovarian cancer tissues and cell lines. MiR-145 inhibits p70S6K1 post-transcriptional expression by binding to its 3'-UTR. The angiogenic factors hypoxia-inducible factor 1 (HIF-1) and vascular endothelial growth factor (VEGF), which are downstream molecules of p70S6K1, are decreased by miR-145 overexpression. P70S6K1 rescues miR-145-suppressed HIF-1 and VEGF …

Blood-Brain Barrier Abnormalities Caused By Hiv-1 Gp120: Mechanistic And Therapeutic Implications., Jean-Pierre Louboutin, David S Strayer

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

The blood-brain barrier (BBB) is compromised in many systemic and CNS diseases, including HIV-1 infection of the brain. We studied BBB disruption caused by HIV-1 envelope glycoprotein 120 (gp120) as a model. Exposure to gp120, whether acute [by direct intra-caudate-putamen (CP) injection] or chronic [using SV(gp120), an experimental model of ongoing production of gp120] disrupted the BBB, and led to leakage of vascular contents. Gp120 was directly toxic to brain endothelial cells. Abnormalities of the BBB reflect the activity of matrix metalloproteinases (MMPs). These target laminin and attack the tight junctions between endothelial cells and BBB basal laminae. MMP-2 and …

Graft Versus Host Disease Of The Brain Following Allogeneic Stem Cell Transplant For Myelodysplastic Syndrome, Janet Yoo, Lindsay Simon, Zi-Xuan Wang, Phd, Lawrence C. Kenyon

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Graft-versus-host disease (GVHD) is a frequent cause of morbidity and mortality following bone marrow transplantation (BMT). GVHD occurs when immune cells transplanted from a non-identical donor (the graft) recognize the transplant recipient (the host) as foreign, thereby initiating an immune reaction that causes disease in the transplant recipient. GVHD, which can present either acutely or chronically, typically involves the skin, gastrointestinal tract, and liver. In contrast, GVHD involving other organs such as the heart and kidney is highly uncommon and involvement of the central nervous system has only very rarely been described in the literature. We report an unusual case …

Lung Carcinoma In The Era Of Personalized Medicine: The Role Of Cytology., Maureen F Zakowski, Marluce Bibbo

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

In 2004, three groups working independently [1–3] , almost simultaneously, reported remarkable findings: that activating mutations in the epidermal growth factor receptor (EGFR) were common in certain lung carcinomas and that these mutations correlated with the response of those lung tumors to therapy with gefitinib and erlotinib, both EGFR tyrosine kinase inhibitors (TKIs). This was the first time driver mutations in lung cancer that responded to targeted therapy had been identified, marking the beginning of a new era of personalized medicine in lung cancer. Prior to the discovery of these mutations, patients had been treated with EGFR TKIs but predicting …

Cop9 Signalosome Component Jab1/Csn5 Is Necessary For T Cell Signaling Through Lfa-1 And Hiv-1 Replication., Shigemi M Kinoshita, Peter O Krutzik, Garry P Nolan

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

To determine critical host factors involved in HIV-1 replication, a dominant effector genetics approach was developed to reveal signaling pathways on which HIV-1 depends for replication. A large library of short peptide aptamers was expressed via retroviral delivery in T cells. Peptides that interfered with T cell activation-dependent processes that might support HIV-1 replication were identified. One of the selected peptides altered signaling, lead to a difference in T cell activation status, and inhibited HIV-1 replication. The target of the peptide was JAB1/CSN5, a component of the signalosome complex. JAB1 expression overcame the inhibition of HIV-1 replication in the presence …

Knockdown Of Ki-67 By Dicer-Substrate Small Interfering Rna Sensitizes Bladder Cancer Cells To Curcumin-Induced Tumor Inhibition., Sivakamasundari Pichu, Swapna Krishnamoorthy, Andrei Shishkov, Bi Zhang, Peter Mccue, Biddanda C Ponnappa

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Transitional cell carcinoma (TCC) of the urinary bladder is the most common cancer of the urinary tract. Most of the TCC cases are of the superficial type and are treated with transurethral resection (TUR). However, the recurrence rate is high and the current treatments have the drawback of inducing strong systemic toxicity or cause painful cystitis. Therefore, it would be of therapeutic value to develop novel concepts and identify novel drugs for the treatment of bladder cancer. Ki-67 is a large nucleolar phosphoprotein whose expression is tightly linked to cell proliferation, and curcumin, a phytochemical derived from the rhizome Curcuma …

The Dermatan Sulfate Proteoglycan Decorin Modulates Α2Β1 Integrin And The Vimentin Intermediate Filament System During Collagen Synthesis., Oliver Jungmann, Katerina Nikolovska, Christian Stock, Jan-Niklas Schulz, Beate Eckes, Christoph Riethmüller, Rick T Owens, Renato V Iozzo, Daniela G Seidler

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Decorin, a small leucine-rich proteoglycan harboring a dermatan sulfate chain at its N-terminus, is involved in regulating matrix organization and cell signaling. Loss of the dermatan sulfate of decorin leads to an Ehlers-Danlos syndrome characterized by delayed wound healing. Decorin-null (Dcn(-/-)) mice display a phenotype similar to that of EDS patients. The fibrillar collagen phenotype of Dcn(-/-) mice could be rescued in vitro by decorin but not with decorin lacking the glycosaminoglycan chain. We utilized a 3D cell culture model to investigate the impact of the altered extracellular matrix on Dcn(-/-) fibroblasts. Using 2D gel electrophoresis followed by mass spectrometry, …

Decorin Protein Core Affects The Global Gene Expression Profile Of The Tumor Microenvironment In A Triple-Negative Orthotopic Breast Carcinoma Xenograft Model., Simone Buraschi, Thomas Neill, Rick T Owens, Leonardo A Iniguez, George Purkins, Rajanikanth Vadigepalli, Barry Evans, Liliana Schaefer, Stephen C Peiper, Zi-Xuan Wang, Renato V Iozzo

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Decorin, a member of the small leucine-rich proteoglycan gene family, exists and functions wholly within the tumor microenvironment to suppress tumorigenesis by directly targeting and antagonizing multiple receptor tyrosine kinases, such as the EGFR and Met. This leads to potent and sustained signal attenuation, growth arrest, and angiostasis. We thus sought to evaluate the tumoricidal benefits of systemic decorin on a triple-negative orthotopic breast carcinoma xenograft model. To this end, we employed a novel high-density mixed expression array capable of differentiating and simultaneously measuring gene signatures of both Mus musculus (stromal) and Homo sapiens (epithelial) tissue origins. We found that …

Kaempferol Nanoparticles Achieve Strong And Selective Inhibition Of Ovarian Cancer Cell Viability., Haitao Luo, Bingbing Jiang, Bingyun Li, Zhaoliang Li, Bing-Hua Jiang, Yi Charlie Chen

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Ovarian cancer is one of the leading causes of cancer death for women throughout the Western world. Kaempferol, a natural flavonoid, has shown promise in the chemoprevention of ovarian cancer. A common concern about using dietary supplements for chemoprevention is their bioavailability. Nanoparticles have shown promise in increasing the bioavailability of some chemicals. Here we developed five different types of nanoparticles incorporating kaempferol and tested their efficacy in the inhibition of viability of cancerous and normal ovarian cells. We found that positively charged nanoparticle formulations did not lead to a significant reduction in cancer cell viability, whereas nonionic polymeric nanoparticles …

Temporal Changes In Innate Immune Signals In A Rat Model Of Alcohol Withdrawal In Emotional And Cardiorespiratory Homeostatic Nuclei., Kate Freeman, Anthony Brureau, Rajanikanth Vadigepalli, Mary M Staehle, Melanie M Brureau, Gregory E Gonye, Jan B Hoek, D Craig Hooper, James S Schwaber

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

BACKGROUND: Chronic alcohol use changes the brain's inflammatory state. However, there is little work examining the progression of the cytokine response during alcohol withdrawal, a period of profound autonomic and emotional upset. This study examines the inflammatory response in the central nucleus of the amygdala (CeA) and dorsal vagal complex (DVC), brain regions neuroanatomically associated with affective and cardiorespiratory regulation in an in vivo rat model of withdrawal following a single chronic exposure.

METHODS: For qRT-PCR studies, we measured the expression of TNF-α, NOS-2, Ccl2 (MCP-1), MHC II invariant chain CD74, and the TNF receptor Tnfrsf1a in CeA and DVC …

Emergence Of Bimodal Cell Population Responses From The Interplay Between Analog Single-Cell Signaling And Protein Expression Noise., Marc R Birtwistle, Jens Rauch, Anatoly Kiyatkin, Edita Aksamitiene, Maciej Dobrzyński, Jan B. Hoek, Walter Kolch, Babatunde A Ogunnaike, Boris N Kholodenko

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

BACKGROUND: Cell-to-cell variability in protein expression can be large, and its propagation through signaling networks affects biological outcomes. Here, we apply deterministic and probabilistic models and biochemical measurements to study how network topologies and cell-to-cell protein abundance variations interact to shape signaling responses.

RESULTS: We observe bimodal distributions of extracellular signal-regulated kinase (ERK) responses to epidermal growth factor (EGF) stimulation, which are generally thought to indicate bistable or ultrasensitive signaling behavior in single cells. Surprisingly, we find that a simple MAPK/ERK-cascade model with negative feedback that displays graded, analog ERK responses at a single cell level can explain the experimentally …

Feasibility Study Of Trans-Cranial Direct Current Stimulation In Presence Of Brain Tumor, Abhilash Nair

Dissertations and Theses

Trans-cranial Direct Current Stimulation has been shown to modulate cortical neuronal activity. Weak constant current is applied to the scalp using electrodes, leading to sub-threshold changes in neuronal membrane potential. Before actual clinical stimulation is performed, such systems are validated by simulating the stimulation and the resulting current flow patterns using finite element solvers. However, the reliability of such models depends upon the accuracy with which the underlying anatomy has been modeled [1]. In earlier studies it has been shown that the current flow patterns within the brain are altered due to the presence of lesioned brain tissues[2]. Here we …

Does The Perception That Stress Affects Health Matter? The Association With Health And Mortality, Abiola O. Keller, Kristin Litzelman, Lauren E. Wisk, Torsheika Maddox, Erika Rose Cheng, Paul D. Creswell, Whitney P. Witt

College of Nursing Faculty Research and Publications

No abstract provided.

The Role Of Sleep In The Enhancement And Impedance Of Episodic Emotional Memories, Rafael De Jesus

Dissertations and Theses

"The current study examined whether sleep enhances the retention of emotionally arousing memories, and whether an emotional memory can be blocked with a single night of sleep deprivation. Using a modified version of a slide show varying in emotionality with respect to the narrative (participants' acting as either a mother or father experiencing the tragic death of their imagined child), 100 (healthy young adult student volunteer) subjects following the experience were randomly assigned to either a normal night of sleep or were variously sleep deprived (in laboratory). After having experienced the emotional event followed immediately by a night of sleep …

Self-Concept Related To Identities And Safer-Sex Cognitions In Young Adults Who Have Lost A Parent To Aids, Marcie Berman

Dissertations and Theses

In-person interviews conducted with 40 English-speaking AIDS-bereaved 18-22 year olds and a control group revealed a significant difference in identities. Data were collected from an AIDS-bereaved group through Rutgers, the State University of New Jersey and an introductory psychology class at Hunter College, City University of New York. Hierarchical Classes (HICLAS; Ceulemans & Van Mechelen, 2005) data analysis uncovered a significant difference in how the groups elaborate their current and ideal selves and their self-cognitions surrounding protected and unprotected sex with both new and ongoing partners. Chi-square analysis showed significant differences in elaboration levels regarding how the AIDS-bereaved viewed unprotected …

Reducing False Positive Feigning Classifications On The Sirs Among Criminal Defendants With A History Of Trauma, Lea Corconan

Dissertations and Theses

Clinical research has demonstrated that individuals with a traumatic history elevate scales on the Structured Interview of Reported Symptoms (SIRS; Rogers, Bagby, & Dickens, 1992) leading to their misclassification as malingerers. Rogers, Payne, Correa, Gillard, and Ross (2009) created the trauma index (TI) by summing 3 SIRS scales to reduce the number of false positives in a severely traumatized sample. The TI was included as an additional criterion in determining feigning. The TI has not been studied using a forensic sample. Fifty-one adult male criminal defendants were identified as malingering by the SIRS with a final sample of 5 definite …

Sensation Seeking, Early Pregnancy And Criminal Justice System Involvement Among Latino Youth, Jillian Jankie

Dissertations and Theses

The present study examined the relationship between the level of sensation seeking, early pregnancy, and criminal justice system involvement among Latino youth. It is a secondary analysis of data that comes from the Boricua Youth Study, an ongoing longitudinal study of Puerto Rican children and preadolescents living in the South Bronx and Puerto Rico. In the current analysis, data were analyzed on 69 participants who had completed measures of sensation seeking, pregnancy and arrests to predict whether sensation seeking would predict arrests and pregnancy at the second time period. Sensation seeking was evaluated at baseline. Early pregnancy and criminal justice …

Perceived Discrimination And Depression As It Relates To Nativity Status Of Students From Immigrant Backgrounds, Melissa Huey

Dissertations and Theses

"Research has found that foreign born immigrants' have better mental and physical health, as well as higher levels of academic achievement, than native born individuals from immigrant families (Vega, et. al., 2004). There is some evidence that perceived discrimination could be a possible explanation for these nativity differences (Juang & Cookston, 2009). The current study tested the hypothesis that native born individuals from immigrant backgrounds will have higher levels of depression than their foreign born counterparts and that native born respondents will report higher levels of perceived discrimination than foreign born. The study also tested whether perceived discrimination mediates the …

Interaction Of Long- And Short-Term Memory On Selective Attention During Timbre Discrimination, Eva Garcia

Dissertations and Theses

The present study investigated the effects of short- and long-term memory on processes of selective attention during timbre discrimination. Pitch served as the distractor dimension, held constant on standard trials and deviating from the standard frequency on distractor trials. Short-term memory was operationalized as levels of covariate context: Within a block of trials, pitch deviants (p=.28) were either absent (baseline condition), varied orthogonally (filtering condition) or systematically (positive and perfect conditions) with timbre values. Long-term memory was operationalized as levels of psychophysical context: Within a block of trials, the range of pitch change was psychophysically equated with timbre change in …

The Effects Of Circadian Rhythm-Related Mood On Recognition And Recall Memory For Emotional Images, Priya Puliyampet

Dissertations and Theses

"Studies have proposed that sleep following the presentation of emotional information aids in the consolidation of memory for the emotional elements of the stimuli. Some of these studies have used a paradigm that may have been affected by the confounding influence of circadian factors. Mood may follow a circadian rhythm and research suggests that mood at the time of recall can influence the content of the memory recalled. This study was meant to determine whether the enhanced memory for emotional stimuli in the morning following a night of sleep is a function of the circadian rhythm of mood. Better recall …

The Influence Of Positive Affect And Anxiety On Attention And Creativity, Rafal Skiba

Dissertations and Theses

The study examined naturally occurring effects of positive affect and anxiety (exam stress at different points in the semester) on attentional performance and creative abilities of undergraduate students. The mood of participants was measured by PANAS and STAI questionnaires. Their attention was tested on the Eriksen Flanker Task, and creativity on the Remote Associates Test (RAT) and Compound Word Problems (CWP). One of the main goals of this study was to verify the prediction based on the Broaden and Build theory of positive resources by Fredrickson (2001). Although the mood manipulation used in this study, that is, placing both testing …

Asperger Syndrome And Blogging; Theory Of Mind And The Extreme Male Brain Theory In Self-Identified Women With Asperger Syndrome, Vanesa Morgan

Dissertations and Theses

No abstract provided.

Increasing Racial And Ethnic Diversity In The Field Of Art Therapy, Yasmine J. Awais

Dissertations and Theses

No abstract provided.

Who Participates In Extreme Obstacle Courses And Why, Stephanie S. Kronenberg

Dissertations and Theses

No abstract provided.

Running Head; Self-Control Deindividuation Context, Jihye Ryu

Dissertations and Theses

No abstract provided.

Sociocultural Predictors Of Perceived Sexism In Urban College Students, Jennifer M. Grant

Dissertations and Theses

No abstract provided.

The Role Of Rejection Sensitivity In Posttraumatic Stress Disorder; A Pilot Investigation, Grace Kiriakos

Dissertations and Theses

"The aim of this secondary data analysis is to explore the hypothetical relationship between rejection sensitivity (RS) level and PTSD symptom severity. We predicted that RS would be positively related to PTSD symptom severity. Pilot data from 39 individuals were analyzed to explore bivariate correlations on indices of RS, PTSD symptom severity, depression symptoms, and Borderline Personality Disorder (BPD) feature endorsement. RS was positively related to PTSD symptom severity at the trend level. Participants were then divided into a group of trauma-exposed individuals and control individuals. Independent samples t-tests were conducted to evaluate mean differences between groups in terms of …