Medicine and Health Sciences Commons^™

Adam10 Regulates Transcription Factor Expression Required For Plasma Cell Function, Natalia S. Chaimowitz, Dae-Joong Kang, Lee M. Dean, Daniel H. Conrad

Microbiology and Immunology Publications

A disintegrin and metalloprotease 10 (ADAM10) is a key regulator of cellular processes by shedding extracellular domains of transmembrane proteins. We have previously demonstrated that deletion of B cell expressed ADAM10 results in changes in lymphoid tissue architecture and impaired germinal center (GC) formation. In this study, mice were generated in which ADAM10 is deleted in B cells following class switch recombination (ADAM10Δ/ΔIgG1-cre+/−mice). Despite normal GC formation, antibody responses were impaired in ADAM10Δ/ΔIgG1-cre+/− mice, implicating ADAM10 in post-GC and extrafollicular B cell terminal differentiation. Surprisingly, plasma cell (PC) numbers were normal in ADAM10Δ/ΔIgG1-cre+/− mice when compared to controls. However, PCs …

Mouse Rc/Btb2, A Member Of The Rcc1 Superfamily, Localizes To Spermatid Acrosomal Vesicles, Jiannan Wang, Maria E. Teves, Xuening Shen, David R. Nagarkatti-Gude, Rex A. Hess, Scott C. Henderson, Jerome F. Strauss Iii, Zhibing Zhang

Obstetrics and Gynecology Publications

Mouse RC/BTB2 is an unstudied protein of the RCC1 (Regulator of Chromosome Condensation) superfamily. Because of the significant remodeling of chromatin that occurs during spermiogenesis, we characterized the expression and localization of mouse RC/BTB2 in the testis and male germ cells. The Rc/btb2 gene yields two major transcripts: 2.3 kb Rc/btb2-s, present in most somatic tissues examined; and 2.5 kb Rc/btb2-t, which contains a unique non-translated exon in its 5′-UTR that is only detected in the testis. During the first wave of spermatogenesis, Rc/btb2-t mRNA is expressed from day 8 after birth, reaching highest levels of expression at day …

Occupational Health And Safety Management Systems Assessment Training, William Brazile, Daniel Autenrieth, Del Sandfort

Safety Health & Industrial Hygiene

An occupational health and safety management system (OHSMS) is required for all Federal agencies by Executive Order 12196 – Occupational safety and health programs for Federal employees. Development of the Occupational Health and Safety Management Systems Assessment Training Manual was supported jointly by the U.S. Veterans Health Administration (VHA) and the Occupational Safety and Health Administration (OSHA). The manual was designed to be used in the training of government personnel on how to assess the OHSMS of Federal establishments and agencies. The manual is organized sequentially through each step in the process of conducting an OHSMS assessment. Trainees start …

Effects Of Standard Labor-Wear On Swimming And Treading Water, John Amtmann, Edd, Les Harris, William K. Spath, Charles Todd, Phd

Applied Health Sciences

We tested the hypothesis that occupational clothing would impair performance during swimming. The sub questions included: (1) Will the standard work wear of a railway worker or laborer impede swimming ability? (2) Will this clothing impact the individual’s ability to tread water? We addressed the research questions with three hypotheses. Analysis showed statistically significant p-values and all three null hypotheses were rejected in favor of the three research hypotheses, showing strong evidence that standard labor wear had adverse effects on 11.43 meter/12.5 yard swim time, water treading time and rate of perceived exertion (RPE) during water treading. The mean swim …

Public Health Impact Of The Hpv Vaccinations: A Research Update, Kathleen Ragan

The Corinthian

In recent years, a large volume of medical and public health literature has been dedicated to the sexually transmitted infection Human Papillomavirus (HPV). The purpose of this paper is to provide a comprehensive assessment of recent empirical investigations which assess the public health impact of HPV and recent HPV vaccinations.

Medical School Watercooler Newsletter - January 1, 2012, Lindsey Lyle

Watercooler Newsletter

This is the January 1, 2012 edition of the Frederick P. Whiddon College of Medicine's newsletter - Watercooler.

Contents Include:

• New Poll Demonstrates Importance of Health Research, Development
• January Med School Café - 'Starting Your Year off Right: Making Resolutions a Reality'
• USA College of Medicine Welcomes Dr. Charles Eric McCathran
• Next Week's DSS - Dr. Richard Cone
• Dr. Fortwendel Awarded Research Grant

University Of South Alabama College Of Medicine Annual Report For 2012-2013, College Of Medicine

COM Annual Reports

The Annual Report for the College of Medicine chronicles the accomplishments of the faculty, students, residents, fellows, and staff in teaching, research, patient care, community service, and scholarly activities during the 2012-2013 academic year.

An Ai Approach To Transform The E-Patient Records Into Clinical Cases For Medical Diagnostic Systems, Rania Hodhod

Faculty Bibliography

A major problem for researchers in medical informatics is the presence of ‘useful’ Electronic Patient Record (EPR) and its use in providing important information to and about the patient’s diseases and treatment. This paper discusses the importance of medical informatics and its role in health procurement branch. It also proposes an AI approach to transform the E-Patient records into clinical cases for medical diagnostic systems. This approach is based on using medical documents to generate E-Patient records, then mapping these records to build medical cases to form a cases repository that would be utilized in a CBR-medical diagnostic system.

Comparing Centralized Vs. Decentralized Nursing Unit Design As A Determinant Of Stress And Job Satisfaction, Jennifer Brown

Faculty Bibliography

The purpose of the study was to determine the effect of the nursing unit design on stress and job satisfaction. The Perceived Stress Scale (PSS) and Demand-Control-Support Questionnaire (DCSQ) were administered to 40 nurse participants to evaluate staff stress, job satisfaction, and perceptions of work environment. In addition, two follow-up focus groups were conducted to expand upon the collected survey data. Results suggest that the centralized floor lends itself to better patient access and professional communication. Further research is needed regarding nurse burn-out and turn-over as it relates to the designed work environment.

Electronic Medical Records, Rania Hodhod

Faculty Bibliography

Electronic Medical Record (EMR) relational database is considered to be a major component of any medical care information system. A major problem for researchers in medical informatics is finding the best way to use these databases to extract valued useful information to and about the patient’s diseases and treatments. Integrating different EMR databases is a great achievement that will improve health care systems. This paper presents an AI approach to extract generic EMR from different resources and transfer them to clinical cases. The utilized approach is based on retrieving different relationships between patients’ different data tables (files) and automatically generating …

Evaluation Of The Role Of Oxidative Stress, Inflammation And Apoptosis In The Pulmonary And The Hepatic Toxicity Induced By Cerium Oxide Nanoparticles Following Intratracheal Instillation In Male Sprague-Dawley Rats, Siva Krishna Nalabotu

Theses, Dissertations and Capstones

The field of nanotechnology is rapidly progressing with potential applications in the automobile, healthcare, electronics, cosmetics, textiles, information technology, and environmental sectors. Nanomaterials are engineered structures with at least one dimension of 100 nanometers or less. With increased applications of nanotechnology, there are increased chances of exposure to manufactured nanomaterials. Recent reports on the toxicity of engineered nanomaterials have given scientific and regulatory agencies concerns over the safety of nanomaterials. Specifically, the Organization for Economic Co-operation and Development (OECD) has identified fourteen high priority nanomaterials for study. Cerium oxide (CeO2) nanoparticles are one among the high priority group. Recent data …

Fueling Sport Performance: Increasing Awareness In Female Collegiate Vegetarian Athletes, Rachael Irene Marie Sofie

Theses, Dissertations and Capstones

Vegetarian diets, when well-planned out, have been shown to meet the needs of female athletes. Although the lifestyle provides many benefits, it does not come without risk. Women athletes, in particular, are of the greatest concern. Energy restriction is common among female collegiate athletes. Because athletes are already converting to and practicing a vegetarian lifestyle, it is necessary that athletes and related sport professionals become aware of the risks and benefits of a vegetarian lifestyle. By increasing knowledge among related personnel, athletes can use vegetarianism as a performance enhancer. Outlining possible implications of the lifestyle will allow female collegiate athletes …

Visualization Of The Passive Sink Phenomenon In Nonexercising Lower Extremity Muscle Using Two Sampling Sites: Consequences For Assessment And Training, Michelle Elyse Copolo

Theses, Dissertations and Capstones

In the past, interest in the application of human performance and testing values has been limited to exercise professionals and those who participate in physical activity at high levels. One common method exercise physiologists employ to determine performance is the assessment of blood lactate concentration [La - ] through sampling. Blood lactate has been studied extensively; however, selection of an optimal sample site for drawing blood lactate is still an ongoing concern for exercise physiologists. Site selection can impact the values of blood [La - ] being reported, which can in turn impact the development of the training prescription. Studies …

It Is Time To Talk About It: Designing A Health Communication Program For Eating Disorder Recovery In An Appalachian University, Erica Lynn Clites

Theses, Dissertations and Capstones

The purpose of this thesis was to evaluate the treatments used in current eating disorder programs and to develop a more effective model using two relevant communication theories: the Transtheoretical Model of Change and Social Networking Theory. Further, the thesis explored the need for greater mental health literacy on Appalachian college and university campuses as it relates to the three major eating disorders defined by the DSMIV-TR: anorexia nervosa, bulimia nervosa, and binge eating disorders. The analysis of current treatment revealed an overemphasis on physical health and quantitative medical progress. Applying both the Transtheoretical Model of Change and Social Networking …

The Role Of Ptip In Breast Cancer, Lina Niu

Theses, Dissertations and Capstones

In the U.S., breast cancer comprises about 30% of all cancer cases (excluding skin cancer) in women. Such a high incidence makes breast cancer a significant health concern, but our understanding of the molecular and cellular mechanisms of this disease is still limited. Growing evidence suggests that the development of human breast cancer may involve epigenetics, which attributes changes in phenotype to mechanisms other than changes in the DNA sequence itself. Histones as the chief proteins of chromatin work on gene expression, and methylation of histone 3 lysine 4 (H3K4) results in transcriptional activation. Lately, Paired box (Pax) trans-activation domain-interacting …

A Mechanistic Study Of S-Adenosyl-L-Methionine Protection Against Acetaminophen Hepatotoxicity, James Michael Brown

Theses, Dissertations and Capstones

Acetaminophen (APAP) toxicity remains the leading cause of drug induced liver failure in the United States. The current therapy for APAP toxicity is N-acetylcysteine (NAC). NAC must be administered within eight hours of APAP overdose for maximum efficacy. That, coupled with the fact that APAP toxicity may not be overtly evident, makes an alternative therapeutic intervention worth exploring. Previous work by our laboratory has demonstrated that S-adenosyl-L-methionine (SAMe) prevents APAP toxicity when given following APAP overdose in C57Bl/6 mice at a level comparable to NAC. The focus of the current work was to examine the mechanistic aspects of this protection …

Factors That Motivate Certified Registered Nurse Anesthetists To Practice Independently In The Appalachian Region, Michael Lee Frame Jr.

Theses, Dissertations and Capstones

Introduction: Independent Certified Registered Nurse Anesthetists (CRNAs) provide anesthesia services without the supervision or medical direction of anesthesiologists in healthcare facilities throughout the United States. The present literature is limited concerning the factors that motivate CRNAs to choose employment in rural independent practice.

Research Hypothesis: In a sample of CRNAs who practice independently in one of the 13 states of the Appalachian region there will be no differences between the CRNAs from Appalachian and Non-Appalachian counties in their responses to the motivation factors of achievement, recognition, responsibility, advancement, work itself, salary, working conditions, company and administrative policies, supervision, and …

Risk Factors For Ventilator-Associated Pneumonia In The Adult Trauma Patient: A Retrospective Case-Control Study From A Level I Trauma Center, Summer L. Chapman

Theses, Dissertations and Capstones

Introduction: Ventilator-Associated Pneumonia (VAP) continues to be a common complication among the adult trauma population. Little advancements have been made to decrease the incidence, suggesting further research is needed to establish modifiable risk factors. The purpose of this study was to test for an associated link between prehospital intubation and the development of VAP in the adult trauma patient.

Methodology: A retrospective, case-control study design was utilized. The sample included 494 adult trauma patients who required endotracheal intubation and mechanical ventilation for a minimum of 24 hours. All patients presented as a priority one or two trauma to the Charleston …

The Use Of Cerium Oxide And Curcumin Nanoparticles As Therapeutic Agents For The Treatment Of Ventricular Hypertrophy Following Pulmonary Arterial Hypertension, Madhukar Babu Kolli

Theses, Dissertations and Capstones

Pulmonary arterial hypertension (PAH) is a progressive and fatal disease characterized by inflammation, increased pulmonary vascular resistance, right ventricular failure and premature death. Monocrotaline (MCT) has been used to induce PAH in laboratory rats. Previous in vitro and in vivo work suggested that cerium oxide (CeO2)-and curcumin nanoparticles exhibit anti-inflammatory activity; however, it is unknown if these materials are effective for the treatment of PAH induced cardiac hypertrophy. To determine the efficacy of CeO2 nanoparticle treatment in preventing MCT-induced RV hypertrophy, male Sprague Dawley rats were divided into one of three groups (control, MCT, or MCT + CeO2 nanoparticle, n=6/group). …

The Effect Of An Elastic Eccentric Extensor Mechanism On Hamstring Function, Anna Elizabeth Sutton

Theses, Dissertations and Capstones

Hamstring strains have a long recovery time, a high rate of recurrence, and are very common injuries in sports. Fatigue, improper warm-up, previous injury, strength imbalance, and poor flexibility have all been linked to hamstring injuries. Initial treatment of the hamstring typically consists of rest, ice, compression, elevation, and pain relief. However, no optimal treatment regimen has been developed based on carefully designed clinical trials. This study tested fourteen college-aged male and female subjects. Their gait was recorded using video tracking software with and without an elastic band (Theraband® Akron, Ohio) attached to the anterior surface of their waist and …

A Case Study Of Athletic Training Educators' Sports-Care Responsibilities, Service, And Professional Advancement In Athletic Training Education Programs, Rachael C. Alley

Theses, Dissertations and Capstones

The purpose of this study was to examine the (1) extent of athletic training faculty members, who bear additional duties for intercollegiate sports care, (2) the beliefs of the faculty members who have intercollegiate sports care responsibilities related to these duties being credited for professional advancement, (3) the beliefs of faculty members related to having sport care responsibilities as part of their employment, and (4) faculty perceptions of intercollegiate sports care responsibilities upon promotion, tenure and contract renewal.

A sample of 655 certified athletic trainers was identified by the Board of Certification with the primary occupation designation of educator from …

Redox Regulation Of Differentiation In Neuroblastoma, Anne Marie Silvis

Theses, Dissertations and Capstones

Neuroblastoma is the most common extracranial solid tumor in childhood, ranking third in prevalence among infant cancers. Despite intensive therapy with surgery, radiation and chemotherapy, there is a high chance of tumor recurrence. These children are therefore given retinoids in the maintenance phase to differentiate the tumor cells and to avoid/lessen this incidence. Although 13-cis retinoic acid (13-cis RA) is currently used to treat neuroblastoma, there is much evidence supporting the prevalence of its metabolite, all-trans retinoic acid (ATRA), to be the main signaling retinoid in vivo.

The SK-N-SH neuroblastoma cell line, which is composed of three sub-types: sympatheoadrenal neuroblasts …

Down-Regulation Of The Transcription Factor Snail In The Placentas Of Patients With Preeclampsia And In A Rat Model Of Preeclampsia, Larissa Federova, Cara Gatto-Weis, Sleiman Smaili, Nauman Khurshid, Joseph I. Shapiro M.D., Deepak Malhorta, Terrence Horrigan

Biochemistry and Microbiology

Background: Placental malfunction in preeclampsia is believed to be a consequence of aberrant differentiation of trophoblast lineages and changes in utero-placental oxygenation. The transcription factor Snail, a master regulator molecule of epithelial-mesenchymal transition in embryonic development and in cancer, is shown to be involved in trophoblast differentiation as well. Moreover, Snail can be controlled by oxidative stress and hypoxia. Therefore, we examined the expression of Snail and its downstream target, e-cadherin, in human normal term, preterm and preeclamptic placentas, and in pregnant rats that developed preeclampsia-like symptoms in the response to a 20- fold increase in sodium intake.

Methods: Western …

Controlled Biomineralization Of Electrospun Poly(Ε-Caprolactone) Fibers For Enhancing Their Mechanical Properties, Jingwei Xie, Shaoping Zhong, Bing Ma, Franklin D. Shuler, Chwee Teck Lim

MIIR Faculty Research

Electrospun polymeric fibers have been investigated as scaffolding materials for bone tissue engineering. However, their mechanical properties, and in particular stiffness and ultimate tensile strength, cannot match those of natural bones. The objective of the study was to develop novel composite nanofiber scaffolds by attaching minerals to polymeric fibers using an adhesive material-the mussel-inspired protein polydopamine-as a "superglue". Herein, we report for the first time the use of dopamine to regulate mineralization of electrospun poly(ε-caprolactone) (PCL) fibers to enhance their mechanical properties. We examined the mineralization of the PCL fibers by adjusting the concentration of HCO3 - and dopamine in …

Extraskeletal Effects Of Vitamin D: Potential Impact On Wv Disease Morbidity And Mortality, Franklin D. Shuler, Dana Lycans, Elias Salloum

Orthopaedics

Vitamin D is an essential nutrient and a secosteroid hormone that regulates many physiologic processes beyond calcium and bone homeostasis. These "extraskeletal" effects are impacted by the circulating levels of the storage form of vitamin D, 25-hydroxyvitamin D3. Levels of vitamin D can be detected after completing a simple 25(OH)D blood test. Vitamin D deficiency (< 30 ng/mL) is associated with a higher risk of many chronic diseases including, but not limited to, fourteen types of cancers, type 1 and 2 diabetes mellitus, obesity, cardiovascular disease, hypertension, stroke, and asthma. This article explores the association between vitamin D deficiency and the burden of chronic diseases in West Virginia.

Nurses' Perception Of Clinical Ladder Programs, Bobbie Baucom

Nursing Theses and Capstone Projects

The purpose of this study was to measure registered nurses' perceptions of clinical ladder programs and to determine what registered nurses consider to be satisfiers in order to keep them at the bedside. Clinical ladder programs reward registered nurses for their advancement in nursing education, nursing research, expert clinical skills, and leadership skills. This study was conducted on 68 post-licensed registered nurses from the ADN to BSN program at Gardner-Webb University. A quantitative descriptive study using an instrument which items measured perceptions of registered nurses' regarding clinical ladder programs was utilized. The results concluded that registered nurses thought autonomy and …

Nurse's Perception Of Factors Associated With Family Satisfaction In The Intensive Care, Phyllis K. Buie

Nursing Theses and Capstone Projects

In health care, family satisfaction has become one of the most important and challenging competitive elements of the industry. The purpose of this study is to determine the factors associated with the Registered Nurse's perception of family satisfaction with services provided during a patient's hospitalization in the intensive care unit (ICU). Registered Nurses, with critical care experience within the past five years, were asked to rate factors that influenced family satisfaction in the intensive care using the Critical Care Family Satisfaction Survey (CCFSS) developed by Thomas Wasser (2001). Watson's Theory of Human Caring was used as the theoretical framework for …

The Effect Of The Timing Of A Hospice Referral And The Perceived Quality Of Care By The Family, Melinda Reep

Nursing Theses and Capstone Projects

Late referrals to hospice can result in less time for the hospice team to perform assessments, establish relationships, and initiate goals. This secondary analysis addresses the effects of the length of stay of a hospice patient and the level of satisfaction reported by their caregivers after the passing of their loved one. The hypothesis of the study is that there is a direct relationship between a family's perception of the value of hospice and the length of stay of their terminally ill loved one. The setting for this secondary analysis was a non-profit hospice in the piedmont of North Carolina. …

Evaluating The Knowledge And Attitudes Regarding Pain Management Among Nurses In A Surgical Setting At An Acute Care Facility, Barbara Turner

Nursing Theses and Capstone Projects

It is estimated that seventy-three million patients undergo surgical procedures each year in the United States. Of these, 80% experience acute post-operative pain, and approximately 20% experience severe pain (Hutchinson, 2007). Benner's Theory from Novice to Expert was used to guide the study, "Evaluating the Knowledge and Attitudes Regarding Pain Management Among Nurses in a Surgical Setting at an Acute Care Facility". A convenience sample of twenty-one nurses in an acute care hospital in Southwestern North Carolina participated in the Knowledge and Attitudes Survey Regarding Pain. Results showed a weak correlation between knowledge and attitudes of pain management and years …

The Effect Of Education On Healthcare Personnel's Knowledge Of Lymphedema Detection And Prevention, Dolly Wilson

Nursing Theses and Capstone Projects

Secondary lymphedema (SLE) is a serious problem for many of the 2.6 million breast cancer survivors in the United States. It is estimated that 28 - 38% of breast cancer survivors develop lymphedema. The five-year incidence ranges from 43% to 94% (Armer, 2010). The purpose of this study was to evaluate the effectiveness of an education program on healthcare personnel's knowledge of the risk of and preventive measures for upper limb lymphedema in breast cancer patients. A sample of healthcare personnel (N = 32) included both trained healthcare personnel and ancillary staff who are employed in a single oncology center …