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2012

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Articles 1981 - 2010 of 7318

Full-Text Articles in Medicine and Health Sciences

A Refractive Surgery Candidate With Optic Nerve Head Cupping., Ivan Goldberg, L Jay Katz, Kaweh Mansouri, Mohammad Pakravan, Shahin Yazdani Jul 2012

A Refractive Surgery Candidate With Optic Nerve Head Cupping., Ivan Goldberg, L Jay Katz, Kaweh Mansouri, Mohammad Pakravan, Shahin Yazdani

Wills Eye Hospital Papers

No abstract provided.


Spectral Properties Of H-Reflex Recordings After An Acute Bout Of Whole-Body Vibration, Kristof Kipp, Samuel T. Johnson, Mark A. Hoffman Jul 2012

Spectral Properties Of H-Reflex Recordings After An Acute Bout Of Whole-Body Vibration, Kristof Kipp, Samuel T. Johnson, Mark A. Hoffman

Exercise Science Faculty Research and Publications

Although research supports the use of whole-body vibration (WBV) to improve neuromuscular performance, the mechanisms for these improvements remain unclear. The purpose of this study was to identify the effect ofWBV on the spectral properties of electrically evoked H-reflex recordings in the soleus (SOL) muscle. The H-reflex recordings were measured in the SOL muscle of 20 participants before and after a bout of WBV. The H-reflexes were evoked every 15 seconds for 150 seconds after WBV. A wavelet procedure was used to extract spectral data, which were then quantified with a principle components analysis. Resultant principle component scores were used …


Weightlifting Performance Is Related To Kinematic And Kinetic Patterns Of The Hip And Knee Joints, Kristof Kipp, Josh Redden, Michelle B. Sabick, Chad Harris Jul 2012

Weightlifting Performance Is Related To Kinematic And Kinetic Patterns Of The Hip And Knee Joints, Kristof Kipp, Josh Redden, Michelle B. Sabick, Chad Harris

Exercise Science Faculty Research and Publications

The purpose of this study was to investigate the correlations between biomechanical outcome measures and weightlifting performance. Joint kinematics and kinetics of the hip, knee, and ankle were calculated while 10 subjects performed a clean at 85% of 1 repetition maximum (1RM). Kinematic and kinetic time-series patterns were extracted with principal components analysis. Discrete scores for each time-series pattern were calculated and used to determine how each pattern was related to body mass–normalized 1RM. Two hip kinematic and 2 knee kinetic patterns were significantly correlated with relative 1RM. The kinematic patterns captured hip and trunk motions during the first pull …


Resourcefulness Training Intervention: Assessing Critical Parameters From Relocated Older Adults’ Perspectives, Abir K. Bekhet, Jaclene Zauszniewski, Denise M. Matel-Anderson Jul 2012

Resourcefulness Training Intervention: Assessing Critical Parameters From Relocated Older Adults’ Perspectives, Abir K. Bekhet, Jaclene Zauszniewski, Denise M. Matel-Anderson

College of Nursing Faculty Research and Publications

The population of American elders is increasing rapidly and relocation to retirement communities has been found to adversely affect their adjustment. This pilot study of 38 relocated elders evaluated, from elders’ perspectives, six critical parameters of a resourcefulness training (RT) intervention designed to help elders adjust to relocation. Within the context of Zauszniewski's theory of resourcefulness, a pre-/post-test design with random assignment to RT or to diversionary activities (DA) was used. Objective questionnaires measured demographic and relocation factors. An intervention evaluation questionnaire was designed and given to the relocated elders in order to assess the six critical parameters—necessity, acceptability, feasibility, …


Transformative Learning Through Study Abroad In Low-Income Countries, Cynthia Foronda, Ruth Ann Belknap Jul 2012

Transformative Learning Through Study Abroad In Low-Income Countries, Cynthia Foronda, Ruth Ann Belknap

College of Nursing Faculty Research and Publications

Study abroad in low-income countries is an emerging trend in nursing education, yet student outcomes vary from positive to negative. Study abroad in low-income countries can be transformative because it has the potential to increase student awareness of socioeconomic relations, structural oppression, and human connectedness. The authors discuss 10 strategies to facilitate transformative learning in students who study abroad.


Evaluation Of A Coping Kit Of Items To Support Children With Developmental Disorders In The Hospital Setting, Jennifer Drake, Norah L. Johnson, Alice V. Stoneck, Deb M. Martinez, Megan Massey Jul 2012

Evaluation Of A Coping Kit Of Items To Support Children With Developmental Disorders In The Hospital Setting, Jennifer Drake, Norah L. Johnson, Alice V. Stoneck, Deb M. Martinez, Megan Massey

College of Nursing Faculty Research and Publications

This study attempted to answer the question, "Do nurses perceive coping kits to be effective at meeting the needs of hospitalized children with developmental disabilities who are at increased risk for challenging behaviors?" A cross-sectional post-test survey study design was used, with a convenience sample of 24 registered nurses at a Midwestern free-standing children's hospital. A coping kit with simple communication cards, social script book, and distraction items (toys) was developed to enhance communication and distract children with developmental disabilities (including autism spectrum disorder) undergoing procedures in the hospital. A modified version of Hudson's (2006) intervention effectiveness survey was used …


Intermediate-Term Mortality And Cardiac Transplantation In Infants With Single-Ventricle Lesions: Risk Factors And Their Interaction With Shunt Type., James S. Tweddell, Lynn A. Sleeper, Richard G. Ohye, Ismee A. Williams, Lynn Mahony, Christian Pizarro, Victoria L. Pemberton, Peter C. Frommelt, Scott M. Bradley, James F. Cnota, Jennifer Hirsch, Paul M. Kirshbom, Jennifer S. Li, Nancy Pike, Michael Puchalski, Chitra Ravishankar, Jeffrey P. Jacobs, Peter C. Laussen, Brian W. Mccrindle, Pediatric Heart Network Investigators, Girish S. Shirali Jul 2012

Intermediate-Term Mortality And Cardiac Transplantation In Infants With Single-Ventricle Lesions: Risk Factors And Their Interaction With Shunt Type., James S. Tweddell, Lynn A. Sleeper, Richard G. Ohye, Ismee A. Williams, Lynn Mahony, Christian Pizarro, Victoria L. Pemberton, Peter C. Frommelt, Scott M. Bradley, James F. Cnota, Jennifer Hirsch, Paul M. Kirshbom, Jennifer S. Li, Nancy Pike, Michael Puchalski, Chitra Ravishankar, Jeffrey P. Jacobs, Peter C. Laussen, Brian W. Mccrindle, Pediatric Heart Network Investigators, Girish S. Shirali

Manuscripts, Articles, Book Chapters and Other Papers

OBJECTIVE: The study objective was to identify factors associated with death and cardiac transplantation in infants undergoing the Norwood procedure and to determine differences in associations that might favor the modified Blalock-Taussig shunt or a right ventricle-to-pulmonary artery shunt.

METHODS: We used competing risks methodology to analyze death without transplantation, cardiac transplantation, and survival without transplantation. Parametric time-to-event modeling and bootstrapping were used to identify independent predictors.

RESULTS: Data from 549 subjects (follow-up, 2.7 ± 0.9 years) were analyzed. Mortality risk was characterized by early and constant phases; transplant was characterized by only a constant phase. Early phase factors associated …


Acute Lymphoblastic Leukemia In A Child With Fanconi’S Anemia, Naureen Mushtaq, Rabia Wali, Zehra Fadoo, Ali Faisal Saleem Jul 2012

Acute Lymphoblastic Leukemia In A Child With Fanconi’S Anemia, Naureen Mushtaq, Rabia Wali, Zehra Fadoo, Ali Faisal Saleem

Department of Paediatrics and Child Health

No abstract provided.


Giant Cell Reparative Granuloma Of Temporal Bone: Case Report Of A 62-Year-Old Male, Muhammad Waqas, Naeem Sultan Ali, Muhammad Zubair Tahir, Syed Ather Enam, Zeeshan-Ud-Din, Mubasher Ikram Jul 2012

Giant Cell Reparative Granuloma Of Temporal Bone: Case Report Of A 62-Year-Old Male, Muhammad Waqas, Naeem Sultan Ali, Muhammad Zubair Tahir, Syed Ather Enam, Zeeshan-Ud-Din, Mubasher Ikram

Section of Otolaryngology, Head & Neck Surgery

Giant cell reparative granuloma (GCRG) is an uncommon non‑neoplastic reactive tumor which occurs almost exclusively within the mandible and maxilla. GCRG of the temporal bone is a rare condition. It has been found to affect predominantly adolescents and adults (age: 10‑25 years). We report a case of a 62‑year‑old male with GCRG of left temporal bone who presented to us with progressive left temporal swelling for 3 months. It was associated with hearing loss. There was no history of trauma. A non‑contrast computed tomography scan brain showed a locally destructive lesion involving squamous temporal bone closely related to the left …


Early Selection According To Repeatability Of Body Measurements Of Turkish Arabian Foals, Süleyman Çilek Jul 2012

Early Selection According To Repeatability Of Body Measurements Of Turkish Arabian Foals, Süleyman Çilek

The Thai Journal of Veterinary Medicine

In this study, body measurements of Turkish Arabian foals at different ages was obtained from Anadolustate farm in Turkey which included body length, cannon bone circumference, heart girth and withers heightmeasurements. Effects of sex, birth month, birth year and age of foal at different growth periods on withers height,body length, heart girth and cannon bone circumference were significant (p<0.05). Effects of environmental factors onall body measurements were standardized additively. Using intra-class correlation method, repeatability was 0.55 forwither height, 0.47 for heart girth, 0.26 for cannon bone circumference for forelimb and 0.31 for body length.According to moderate level results of repeatability for especially heart girth and wither height measurements,selection of Turkish Arabian foals may be conducted at an early age according to body measurements at 6 months ofage.


Genetic Associations Between Stillbirth, Total Number Of Piglets Born And Gestation Length In A Commercial Pig Farm, Nalinee Imboonta, Prapatsara Kuhaaudomlarp Jul 2012

Genetic Associations Between Stillbirth, Total Number Of Piglets Born And Gestation Length In A Commercial Pig Farm, Nalinee Imboonta, Prapatsara Kuhaaudomlarp

The Thai Journal of Veterinary Medicine

Data of purebred Landrace, Large White and their crossbreds taken from a commercial swine farm locatedin the north-eastern part of Thailand were used to evaluate the genetic associations of stillbirth with the total numberof piglets born and gestation length. The analyses were base on 28,275 litters born from 2002 to 2006. Analysis wascarried out with a multivariate animal model using average information REML procedures. Heritability estimates forstillbirth, the total number of piglets born and gestation length were 0.03±0.01, 0.03±0.01 and 0.16±0.01, respectively.The genetic correlation between the total number of piglets born and stillbirth was positive. The genetic correlationbetween the total …


Molecular Characterization And Detection Of Babesia Canis Vogeli In Asymptomatic Roaming Dogs In Chiang Mai, Thailand, Kittisak Buddhachat, Orachon Meesong, Korakot Nganvongpanit, Maslin Osathanunkul, Siriwadee Chomdej Jul 2012

Molecular Characterization And Detection Of Babesia Canis Vogeli In Asymptomatic Roaming Dogs In Chiang Mai, Thailand, Kittisak Buddhachat, Orachon Meesong, Korakot Nganvongpanit, Maslin Osathanunkul, Siriwadee Chomdej

The Thai Journal of Veterinary Medicine

The purpose of this study is to detect Babesia canis by using 18S rDNA amplification in order to confirm thepresence of the agents and to characterize molecularly the Thai B. canis. Three sets of primers, a Babesia canis-specificprimer (BcSP), Piroplasm-specific primer (PSP) and Babesia species-specific primer (BsSP), were tested for sensitivity.The results showed that BcSP and PSP were 50 times more sensitive than BsSP. Both BcSP and PSP were specificenough to detect this parasite in asymptomatic dogs. Peripheral blood samples were collected from 102 asymptomaticdogs residing in Chiang Mai and assayed with a light microscope and PCR by using BcSP …


Seasonal Monitoring Of Dengue Infection In Aedes Aegypti And Serological Feature Of Patients With Suspected Dengue In 4 Central Provinces Of Thailand, Jakkrawarn Chompoosri, Usavadee Thavara, Apiwat Tawatsin, Surapee Anantapreecha, Padet Siriyasatien Jul 2012

Seasonal Monitoring Of Dengue Infection In Aedes Aegypti And Serological Feature Of Patients With Suspected Dengue In 4 Central Provinces Of Thailand, Jakkrawarn Chompoosri, Usavadee Thavara, Apiwat Tawatsin, Surapee Anantapreecha, Padet Siriyasatien

The Thai Journal of Veterinary Medicine

This study aimed to determine seasonal dengue infection rates in Ae. aegypti mosquitoes and dengueinfection in suspected patients in 4 central provinces of Thailand. Ae. aegypti mosquitoes collected during threeseasons and blood specimens taken from patients with suspected dengue were detected and serotyped for dengueviruses using RT-PCR. The biting behavior of Ae. aegypti females was studied by 24-hour mosquitoes collection once amonth using human bait for determination of seasonal biting rate. Dengue infection rates in Ae. aegypti femalesobtained from all the 4 provinces were highest in hot season and varied from place to place ranging from 64.4% to77.5%, whereas morbidity …


The Use Of Epoetin-Α In Revision Knee Arthroplasty., Lawrence A Delasotta, Ashwin V Rangavajjula, Michael L Frank, Jamie L Blair, Fabio R Orozco, Alvin C Ong Jul 2012

The Use Of Epoetin-Α In Revision Knee Arthroplasty., Lawrence A Delasotta, Ashwin V Rangavajjula, Michael L Frank, Jamie L Blair, Fabio R Orozco, Alvin C Ong

Department of Surgery Faculty Papers

Introduction. To evaluate the efficacy of epoetin-α prior to revision total knee arthroplasty, we hypothesized that epoetin-α will reduce blood transfusion. Methods. Eighty-one patients were compared in this retrospective review; twenty-eight patients received our dosing regimen. All patients were mildly anemic. Epoetin-α to control (1 : 2) patient matching occurred so that one of two attending surgeons, gender, BMI, complexity of surgery, ASA score, and age were similar between groups. The clinical triggers for blood transfusion during or after the procedure were determined based on peri- and postoperative hemoglobin levels, ASA score, and/or clinical symptoms consistent with anemia. Blood salvage …


Melanotic Neuroectodermal Tumour Of Infancy: A Rare Brain Tumour Of Childhood, Muhammad Babar Khan, Delvene Soares, Muhammad Zubair Tahir, Rajesh Kumar, Khurram Minhas, Muhammad Ehsan Bari Jul 2012

Melanotic Neuroectodermal Tumour Of Infancy: A Rare Brain Tumour Of Childhood, Muhammad Babar Khan, Delvene Soares, Muhammad Zubair Tahir, Rajesh Kumar, Khurram Minhas, Muhammad Ehsan Bari

Medical College Documents

Melanotic neuroectodermal tumour of infancy is a rare, mostly benign but locally aggressive tumour of neural crest cell
origin occurring in infants. The most commonly affected anatomic site is the maxilla. Such tumours of the brain and skull are very rare. We present the case of an 8 months old baby girl whose presenting complaint was a swelling in the scalp for 6 months. She was otherwise asymptomatic. CT imaging confirmed the presence of an osteolytic tumour in the anterior parasagittal skull with dural involvement. The tumour was surgically excised enbloc. The patient has been well at 2 years follow-up …


Diffusion Weighted Mr Imaging Of Ring Enhancing Brain Lesions, Muhammad Shahbaz Alam, Zafar Sajjad, Muhammad Azeemuddin, Zahid Anwar Khan, Fatima Mubarak, Waseem Akhtar Jul 2012

Diffusion Weighted Mr Imaging Of Ring Enhancing Brain Lesions, Muhammad Shahbaz Alam, Zafar Sajjad, Muhammad Azeemuddin, Zahid Anwar Khan, Fatima Mubarak, Waseem Akhtar

Department of Radiology

Objective: To evaluate the role of diffusion weighted imaging in differentiating the cause of ring enhancing brain lesions.

Study Design: Analytical, descriptive study.

Place and Duration of Study: Department of Radiology, The Aga Khan University Hospital, Karachi, from March 2007 to July 2011. Methodology: Diffusion weighted imaging (DWI) was performed on 37 patients having ring enhancing lesions on their post-contrast brain MRI scans. These lesions were characterized into neoplastic and abscess cavity on the basis of diffusion restriction. Correlation of all these findings was done with histopathology obtained in all these patients. Sensitivity, specificity, positive and negative predictive values and …


Acute And Subchronic Toxicity Study Of Ardisia Ellipica Thunb. Fruit Extract, Nalinphat Saktiyasunthorn, Songpol Chivapat, Pornchai Sincharoenpokai, Anudep Rungsipipat, Nongnuth Maneechai, Boonyanee Suphaphon, Aussavashai Shuayprom Jul 2012

Acute And Subchronic Toxicity Study Of Ardisia Ellipica Thunb. Fruit Extract, Nalinphat Saktiyasunthorn, Songpol Chivapat, Pornchai Sincharoenpokai, Anudep Rungsipipat, Nongnuth Maneechai, Boonyanee Suphaphon, Aussavashai Shuayprom

The Thai Journal of Veterinary Medicine

Several plants species in the genus Ardisia contained various biologically active compounds. Ram Yai orPilangkasa (Ardisia elliptica Thunb.) has been used in traditional medicines. The objective of this study was to evaluatethe safety of ethanolic extract of A. elliptica fruit in animal models by oral administration. Acute toxicity test in miceby gavage the extract at each dose of 2.5 g/kg twice revealed no abnormal signs, mortality and gross lesion of vitalorgans. Subchronic toxicity study was investigated in one hundred Wistar rats separated into five groups, each oftwenty (ten males and ten females). Two control groups received distilled water and 0.5% …


Neospora Caninum And Toxoplasma Gondii Antibodies In Captive Elephants (Elephaus Maximus Indicus) In Kanchanaburi Province, Jitbanjong Wiengcharoen, Weerapan Nokkaew, Samart Prasithpon, Pornsak Prasomtong, Yaowalark Sukthana Jul 2012

Neospora Caninum And Toxoplasma Gondii Antibodies In Captive Elephants (Elephaus Maximus Indicus) In Kanchanaburi Province, Jitbanjong Wiengcharoen, Weerapan Nokkaew, Samart Prasithpon, Pornsak Prasomtong, Yaowalark Sukthana

The Thai Journal of Veterinary Medicine

Although neosporosis has long been detected in several wildlife species from different parts of the world,until now there has been no report of Neospora caninum infection in elephants in any country of the world. Serumsamples of 115 captive elephant (Elephas maximus indicus) from the westernmost province of Kanchanaburi, Thailandwere investigated for antibodies to N. caninum and Toxoplasma gondii. Antibodies of N. caninum were detected by thecompetitive ELISA (cELISA) test and T.gondii by the Latex agglutination (LAT) test. The prevalence of T. gondiiantibodies was 13.04% (15/115), while anti-N. caninum was 33.04% (38/115). Only 7/115 (6.09%) were positive forboth parasites. Our study …


Ecg Quiz, Chollada Buranakarl, Kittipong Tachampa Jul 2012

Ecg Quiz, Chollada Buranakarl, Kittipong Tachampa

The Thai Journal of Veterinary Medicine

No abstract provided.


Ophthalmology Snapshot, Nalinee Tuntivanich Jul 2012

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.


What Is Your Diagnosis, Pranee Tuntivanich, Suwicha Chuthatep Jul 2012

What Is Your Diagnosis, Pranee Tuntivanich, Suwicha Chuthatep

The Thai Journal of Veterinary Medicine

No abstract provided.


Multilevel Analysis Of Physical Activity Among Us Adults Across Us Census Regions: The Role Of Environmental Contexts, Sariyamon Tiraphat Jul 2012

Multilevel Analysis Of Physical Activity Among Us Adults Across Us Census Regions: The Role Of Environmental Contexts, Sariyamon Tiraphat

Health Services Research Dissertations

This study uses nationally representative data to explain the variation in physical activity among U.S. adults across four geographic regions of Northeast, Midwest, South, and West. The purpose of the study is to investigate whether environmental conditions inclusive of natural amenity, built environment, urbanization, crime, and social economic neighborhood are associated with physical activity and whether these associations are modified by geographic region. The study also investigates whether the influences of environmental contexts vary by subgroup and whether they are modified by geographic region. Determining regional factors that influence physical activity is essential to planning appropriate physical activity interventions.

The …


Atypical Pathogens Causing Community-Acquired Pneumonia In Adults., Ali Bin Sarwar Zubairi, Afia Zafar, Nawal Salahuddin, Ahmed Suleman Haque, Shahan Waheed, Javaid Khan Jul 2012

Atypical Pathogens Causing Community-Acquired Pneumonia In Adults., Ali Bin Sarwar Zubairi, Afia Zafar, Nawal Salahuddin, Ahmed Suleman Haque, Shahan Waheed, Javaid Khan

Department of Medicine

Objective: To determine the frequency of community-acquired respiratory pathogens with special focus on atypical organisms in patients presenting to a tertiary care facility with community-acquired pneumonia (CAP).

Methods: The descriptive study on adult patients was conducted from February 2007 to March 2008 at the Aga Khan University Hospital, Karachi. It comprised 124 consenting patients of age 16 and above who presentd with a diagnosis of community-acquired pneumonia. The diagnostic modalities used were based on significant changes in antibody titer or persisting high antibody titers in the case of Mycoplasma pneumoniae and Chalmydia pneumoniae infections, or bacterial antigen in urine, in …


Long-Term Effect Of Carotid Surgery In Asymptomatic Stenosis., Muhammad Faisal Wadiwala, Ayeesha Kamran Kamal Jul 2012

Long-Term Effect Of Carotid Surgery In Asymptomatic Stenosis., Muhammad Faisal Wadiwala, Ayeesha Kamran Kamal

Department of Medicine

No abstract provided.


Attitudes Toward Workplace Mobbing In Slovenian Research Organisations, Brigita Horvat, Milan Pagon Jul 2012

Attitudes Toward Workplace Mobbing In Slovenian Research Organisations, Brigita Horvat, Milan Pagon

All Works

Creating a "mobbing-safe" working environment can be a real challenge; to achieve it, a better understanding of related constructs is necessary. To obtain insight into researchers' attitudes toward workplace mobbing and behavioural intentions, we used a case scenario method. The results show that respondents, on average, consider the mobbing cases presented in a questionnaire to be very serious. Their attitudes and behavioural intentions depend more on their opinion about colleagues' attitudes and related intentions and less on their own, which shows a lower integrity than desired. Low willingness to report the violator, lenient discipline sanctions and the absence of proper …


Role Of Matrix Metalloproteinases In Animals, Sariya Asawakarn, Tanong Asawakarn Jul 2012

Role Of Matrix Metalloproteinases In Animals, Sariya Asawakarn, Tanong Asawakarn

The Thai Journal of Veterinary Medicine

Matrix metalloproteinases are a family of calcium-dependent and zinc-dependent endopeptidases, which excrete from many cell types. These enzymes can hydrolyze extracellular matrix proteins. The most important inhibitor of MMPs is tissue inhibitors of metalloproteinases (TIMPs). The imbalance between MMPs and TIMPs causes pathological condition in human and animals. This review focuses on structural, role and several reports of MMPs in horse, dog, cat, fish and avian. Over production of MMPs is associated with tissue destruction and erosion of cartilage in equine osteoarthritis, especially MMP-2 and MMP-9. In canine and feline, many evidences reported about MMPs and cancer such as MMP-2 …


Risk Factors Of Subclinical Mastitis In Small Holder Dairy Cows In Khon Kaen Province, Chaiwat Jarassaeng, Suneerat Aiumlamai, Chalong Wachirapakorn, Mongkol Techakumphu, Jos P.T.M. Noordhuizen, Anton C. Beynen, Siriwat Suadsong Jul 2012

Risk Factors Of Subclinical Mastitis In Small Holder Dairy Cows In Khon Kaen Province, Chaiwat Jarassaeng, Suneerat Aiumlamai, Chalong Wachirapakorn, Mongkol Techakumphu, Jos P.T.M. Noordhuizen, Anton C. Beynen, Siriwat Suadsong

The Thai Journal of Veterinary Medicine

This cross-sectional study was introduced to determine the risk factors of subclinical mastitis in dairy farms. Sixteen small holder farms and 285 milking cows in Khon Kaen province were selected for the study. The mastitis investigation, feeding information and clinical inspection were applied in the farm visits. Milk samples from each quarter with a CMT score of 3 and bulk milk were collected for bacterial identification and somatic cell counts. The prevalence of subclinical mastitis and associated factors were analyzed. Results found that the prevalence of subclinical mastitis was 36.14%. Major microorganisms isolated from infected quarters were Streptococcus spp. and …


Cloning And Prokaryotic Expression Of Cdnas From Hepatitis E Virus Structural Gene Of The Sw189 Strain, Bao-Cheng Hao, Xi Lan, Xiao-Yong Xing, Hai-Tao Xiang, Feng-Qin Wen, Yu-Yao Hu, Yong-Hao Hu, Jian-Ping Liang, Ji-Xing Liu Jul 2012

Cloning And Prokaryotic Expression Of Cdnas From Hepatitis E Virus Structural Gene Of The Sw189 Strain, Bao-Cheng Hao, Xi Lan, Xiao-Yong Xing, Hai-Tao Xiang, Feng-Qin Wen, Yu-Yao Hu, Yong-Hao Hu, Jian-Ping Liang, Ji-Xing Liu

The Thai Journal of Veterinary Medicine

It is necessary to study the second open reading frame (open reading frame 2, ORF2) and antigenic peptidescharacteristic of genotype 4 of HEV, and create one kind of high sensitivity and specificity of anti-HEV IgM detectionkit. The objective of this study was to obtain recombinant antigen for development of anti-HEV ELISA method andvaccine against hepatitis E virus infection. A 728 base cDNA was collected from 5'-terminus of open reading frame 2(ORF2) among epidemic hepatitis E virus (HEV) isolated from Gansu, Western China. The fragment was digestedwith Not I and Nco I, and inserted into vector pET32a(+). The recombinant plasmid was …


A Comparative Study On The Efficacy Of Four Semen Extenders And Thawing By Seminal Plasma On The Quality Of Frozenthawed Boar Semen, Chonthida Rienprayoon, Chonlapat Klangnak, Suttiporn Onton, Chanyuth Tretipskul, Padet Tummaruk Jul 2012

A Comparative Study On The Efficacy Of Four Semen Extenders And Thawing By Seminal Plasma On The Quality Of Frozenthawed Boar Semen, Chonthida Rienprayoon, Chonlapat Klangnak, Suttiporn Onton, Chanyuth Tretipskul, Padet Tummaruk

The Thai Journal of Veterinary Medicine

The present study investigated the influence of four different commercial semen extenders used during theholding time and the use of seminal plasma as a thawing medium on the frozen-thawed (FT) boar semen quality.Sperm-rich fractions were collected from 11 boars and were divided into four groups according to the type of semenextender used during the holding time (A, B, C and D). The extended semen was cooled down and centrifuged. Thesperm pellets were diluted in lactose-egg yolk extender plus glycerol and Equex STM Paste. The semen were loadedinto 0.5 ml straws and frozen. Thawing was achieved by immersing the straws in …


The Depletion Of Intracellular Glutamine By Methionine Sulfoximine On Amino Acid Uptake In Placental Cells (Bewo), Boonrit Thongsong Jul 2012

The Depletion Of Intracellular Glutamine By Methionine Sulfoximine On Amino Acid Uptake In Placental Cells (Bewo), Boonrit Thongsong

The Thai Journal of Veterinary Medicine

The intracellular concentration of glutamine is very high where it plays a central role as a ready source ofnitrogen, carbon and energy in various metabolic processes. The purpose of the present study was to investigate theinfluence of intracellular glutamine status on amino acid uptake activity in placental choriocarcinoma (BeWo) cells,analogous to normal placental trophoblasts. Intracellular glutamine was depleted by culturing the cells in regularmedium without glutamine and by treating with 2 mM methionine sulfoximine (MSX), an inhibitor of glutaminesynthetase, for 16 hours. The uptake of various amino acids was measured by the use of appropriate substrates andionic conditions. When cultured …