Medicine and Health Sciences Commons^™

Adoption Of A Heart Healthy Diet In Rural Eastern Kentucky, Kevin Luley

Kaleidoscope

No abstract provided.

An Examination Of Maternal Stress, Inflammatory Markers, And Preterm Labor In Pregnant Women, Cecilia Boateng

Kaleidoscope

No abstract provided.

Morphological And Genetic Activation Of Microglia After Diffuse Traumatic Brain Injury In The Rat, Tuoxin Caou, Amanda M. Lisembee, Kelley D. Hall, James R. Pauly, Jonathan Lifshitz

Kaleidoscope

Traumatic brain injury (TBI) survivors experience long, term post-traumatic morbidities. In diffuse brain injured rats, a chronic sensory sensitivity to whisker stimulation models agitation in brain injury survivors and provides anatomical landmarks across the whisker-barrel circuit to evaluate traumatic neuropathology. As a consequence of TBI, acute and chronic microglial activation can contribute to degenerative and reparative events underlying post-traumatic morbidity. Here, we hypothesize that delayed microglial activation is concomitant with neuroplastic change after diffuse brain injury in the rat, by examining differential microglial activation states and neuroplasticity through gene and protein expression. Adult male, Sprague-Dawley rats were subjected to a …

Quantification And In Vitro Analysis Of Nanolipoproteins (Nlps) Containing Adjuvants, Purna Venkataraman, Craig Blanchette, Nicholas O. Fischer

STAR Program Research Presentations

Nanolipoprotein particles (NLPs) self-assemble into nanoscale structures that can be used as vaccines or drug delivery agents. Due to the nature of the NLPs, a variety of immune stimulating compounds or adjuvants can be readily incorporated into NLPs: a characteristic difficult to engineer into most other nanoscale platforms. In light of this, a method for quantifying the amount adjuvant actually incorporated into NLPs is a question of high importance. Through the use of reverse phase High-Performance Liquid Chromatography (HPLC) and an Evaporative Light Scattering Detector (ELSD), standard curves can be constructed by analyzing mixtures of NLP components of known concentration, …

College Drinking: A Call To Physicians, Shawn Sorrel, Hatim A. Omar

Pediatrics Faculty Publications

The aim of this paper was to review literature related to prevalence of drinking on college campuses and current prevention practices targeting this population. This paper focuses on current data surrounding alcohol use and misuse. It further discusses strategies which have been successful to prevent alcohol abuse. Finally, it discusses the need for further research to determine the role of the physician in alcohol prevention at university student health services.

A Change In Pre-Participation Exam Guidelines For Student Athletes In The State Of Mississippi, Alisha Tenhet

MSN Research Projects

Despite the current requirements for pre-participation physical exams (PPE) in Mississippi, student athletes medically cleared during PPEs continue to experience sudden cardiac death from undiagnosed cardiac abnormalities. Research states that one in every 200,000 high school athletes experiences sudden cardiac death, the majority of which are attributed to structural heart problems. Although most high school athletes are required to complete a PPE, the concern is that physical exams alone are not enough to identify cardiac abnormalities. The purpose of this study is to determine if student athletes are appropriately screened for cardiac risks. The researchers compared current practices with existing …

Prospective Patterns And Correlates Of Quality Of Life Among Women In Substance Abuse Treatment, Elizabeth M. Tracy, Alexandre Laudet, Meeyoung Oh Min, Hyunsoo Kim, Suzanne Brown, Min Kyoung Jun, Lynn Singer

Social Work Faculty Publications

Background Quality of life (QOL) is increasingly recognized as central to the broad construct of recovery in sub- stance abuse services. QOL measures can supplement more objective symptom measures, identify specific service needs and document changes in functioning that are associated with substance use patterns. To date however, QOL remains an under investigated area in the addictions field, especially in the United States.

Methods This study examines patterns and predictors of QOL at 1 and 6 months post treatment intake among 240 women enrolled in substance abuse treatment in Cleveland, Ohio. The World Health Organization Quality of Life (WHOQOL-BREF) measure …

Aortic Valve Perforation Diagnosed With Use Of 3-Dimensional Transesophageal Echocardiography, Nitya Alluri, Simi Kumar, Ravi Marfatia, Pravin Patil, Jason Ryan, Erick Avelar

Articles - Patient Care

A 62-year-old man presented with acute decompensated heart failure. His medical history included heart failure with preserved ejection fraction and infective endocarditis. Two-dimensional transthoracic echocardiography (TTE) showed eccentric aortic insufficiency, the mechanism and severity of which could not be accurately determined because multiple jets were present (Fig. 1A and 1B). Three-dimensional (3D) transesophageal echocardiography (TEE) of the aortic valve showed major perforations in the right coronary and noncoronary cusps and small perforations in the left coronary cusp; moderate thickening was consistent with prior endocarditis (Fig. 1C and 1D). Visual examination of the excised aortic valve confirmed these findings (Fig. 2).

Fast Rebinding Increases Dwell Time Of Src Homology 2 (Sh2)-Containing Proteins Near The Plasma Membrane, Dongmyung Oh, Mari Ogiue-Ikeda, Joshua A. Jadwin, Kazuya Machida, Bruce J. Mayer, Ji Yu

UCHC Articles - Research

Receptor tyrosine kinases (RTKs) control a host of biological functions by phosphorylating tyrosine residues of intracellular proteins upon extracellular ligand binding. The phosphotyrosines (p-Tyr) then recruit a subset of ∼100 Src homology 2 (SH2) domain-containing proteins to the cell membrane. The in vivo kinetics of this process are not well understood. Here we use total internal reflection (TIR) microscopy and single-molecule imaging to monitor interactions between SH2 modules and p-Tyr sites near the cell membrane. We found that the dwell time of SH2 modules within the TIR illumination field is significantly longer than predictions based on chemical dissociation rate constants, …

Family-Based Genome-Wide Association Study Of Frontal Theta Oscillations Identifies Potassium Channel Gene Kcnj6, Victor M. Hesselbrock

UCHC Articles - Research

Event-related oscillations (EROs) represent highly heritable neuroelectric correlates of cognitive processes that manifest deficits in alcoholics and in offspring at high risk to develop alcoholism. Theta ERO to targets in the visual oddball task has been shown to be an endophenotype for alcoholism. A family-based genome-wide association study was performed for the frontal theta ERO phenotype using 634583 autosomal single nucleotide polymorphisms (SNPs) genotyped in 1560 family members from 117 families densely affected by alcohol use disorders, recruited in the Collaborative Study on the Genetics of Alcoholism. Genome-wide significant association was found with several SNPs on chromosome 21 in KCNJ6 …

Interleukin-1Β In Central Nervous System Injury And Repair, Nicole A. Jackman, Sandra J. Hewett, Robert J. Claycomb

UCHC Articles - Research

Acute inflammation is a self-limiting, complex biological response mounted to combat pathogen invasion, to protect against tissue damage, and to promote tissue repair should it occur. However, unabated inflammation can be deleterious and contribute to injury and pathology. Interleukin-1β (IL-1β), a prototypical “pro-inflammatory” cytokine, is essential to cellular defense and tissue repair in nearly all tissues. With respect to brain, however, studies suggest that IL-1β has pleiotrophic effects. It acts as a neuromodulator in the healthy central nervous system (CNS), has been implicated in the pathogenic processes associated with a number of CNS maladies, but may also provide protection to …

Dance/Movement Therapy And Autism: A Case Study, Katherine Ann Porter

Creative Arts Therapies Theses

This thesis explores the development of the therapeutic relationship with a child with autism through dance/movement therapy. The characteristics of Autism Spectrum Disorder effect social interactions, communication, behavior and interests. This means there is difficulty engaging and maintaining reciprocal social interactions, and trouble with receptive and expressive language. The purpose of this study is to describe how movement mirroring in dance/movement therapy affects the development of the therapeutic relationship with a child with autism, through the analysis of the Ways of Seeing approach.

The data collected in this clinical case study consisted of video tapped sessions and Laban Movement Analysis …

Need For General Surgeons In Kentucky’S Communities, Willie Bates

Center of Excellence in Rural Health Presentations

Objective

Examine the distribution of general surgeons and need for them throughout Kentucky and their characteristics in terms of practice location and practice type, e.g., community-based private practice, hospital based, and contracted.

Methods

A review of policy and other research literature was conducted to determine trends in the availability of general surgeons for community hospitals and high need rural areas. Data for 2011 from the Kentucky Board of Medical Licensure was the main source for this study. Data showing the distribution of general surgeons were coded for location by county using the 2003 Rural-Urban Continuum Codes.

Results

As a state …

Molecular Mechanisms Of Aging And Immune System Regulation In Drosophila., Ioannis Eleftherianos, Julio C. Castillo

Microbiology, Immunology, and Tropical Medicine Faculty Publications

Aging is a complex process that involves the accumulation of deleterious changes resulting in overall decline in several vital functions, leading to the progressive deterioration in physiological condition of the organism and eventually causing disease and death. The immune system is the most important host-defense mechanism in humans and is also highly conserved in insects. Extensive research in vertebrates has concluded that aging of the immune function results in increased susceptibility to infectious disease and chronic inflammation. Over the years, interest has grown in studying the molecular interaction between aging and the immune response to pathogenic infections. The fruit fly …

Parent-Clinician Communication Intervention During End-Of-Life Decision Making For Children With Incurable Cancer., Pamela S. Hinds, Linda L. Oakes, Judy Hicks, Brent Powell, Deo K. Srivastava, Justin N. Baker, Sheri L. Spunt, Nancy K. West, Wayne L. Furman

Pediatrics Faculty Publications

Background: In this single-site study, we evaluated the feasibility of a parent-clinician communication intervention designed to: identify parents' rationale for the phase I, do-not-resuscitate (DNR), or terminal care decision made on behalf of their child with incurable cancer; identify their definition of being a good parent to their ill child; and provide this information to the child's clinicians in time to be of use in the family's care.

Methods: Sixty-two parents of 58 children and 126 clinicians participated. Within 72 hours after the treatment decision, parents responded to 6 open-ended interview questions and completed a 10-item questionnaire about the end-of-life …

Be Fit To Ski; A Periodized Approach To Alpine Skiing Fitness, Susan Kramer

Graduate Theses

Alpine skiing is considered an explosive sport, requiring high-intensity repetitive muscular action. From the eighties through the early two thousands, studies have revealed the high demands placed on elite ski racers, although limited research has been conducted using recreational skiers. Expert recreational skiers and professional ski instructors are also exposed to high levels of muscular and cardiorespiratory stress, which is why it is beneficial to have a guideline for training and conditioning, much like other team and individual athletes. To date, there are few comprehensive training programs that treat the skier and professional ski instructor as an athlete who needs …

Creating A Model Antigen System To Test The Mechanism Of Gcc-Specific Tolerance, Patrick Ihejirika

Summer Training Program in Cancer Immunotherapy

Immunotherapeutics for colorectal cancer have been under investigation for decades. Unfortunately, a recent meta-analysis has revealed only a 1% response rate for all immunotherapeutic trials in colorectal cancer, highlighting the need for improved immunotherapeutic target antigens which are tumor-specific, immunogenic, and shared by patients. However, to produce immunological responses targeting tumor/self antigens, one has to overcome tolerance, which has constituted an obstacle to previous studies. Guanylyl cylcase C is the index cancer-mucosa antigen, an emerging class of tumor antigens in colorectal cancer. As a mucosa-restricted antigen, GCC is amenable to immunological targeting and uniquely suited for immunotherapy of colorectal cancers. …

Identifying Neurotransmitter Spill-Over In Hippocampal Field Recordings, Emily Stone, Katie Hoffman, Michael P. Kavanaugh

Biomedical and Pharmaceutical Sciences Faculty Publications

A model of synaptic and extra-synaptic excitatory signaling in the hippocampus is presented. The model is used to analytically evaluate the potential contributions of homosynaptic and heterosynaptic glutamate spill-over to receptor signaling during an electrophysiological experiment in which glutamate transporters are pharmacologically blocked. Inhibition of glutamate uptake selectively prolongs the decay kinetics of the second field excitatory postsynaptic potential evoked by paired pulse stimulation of Schaffer collateral axons in area CA1. The model includes AMPA and NMDA glutamate receptors, and the removal of glutamate by transporters and diffusion. We establish analytically that the prolongation cannot be caused by local …

Louisville / Jefferson County Hazardous Material Commodity Flow Analysis, Dr. Vijay Golla, Dr. Ritchie D. Taylor, Jonathan Suhl, Jacob Eagleson, Prachi Chavan, Roni Grigsby, Jim Bottom

Public Health Faculty Publications

This report presents the results of a Hazardous Material Commodity Flow Analysis for the Louisville Metro area and Jefferson County. Study components were conducted by Western Kentucky University in partnership with Louisville / Jefferson County Emergency Management Agency. The study area was focused in Louisville and Jefferson County, Louisville Metro area, and included monitoring in Bullet County and Oldham County, as shown in Figure 1.1. As part of the study, hazardous material transport was monitored at the following sites: Interstate-64 (I-64), East and West bound lanes at The Belvedere; I-64, East and West bound lanes at Blankenbaker exit location; Interstate-65 …

Neural Basis Of The Potentiated Inhibition Of Repeated Haloperidol And Clozapine Treatment On The Phencyclidine-Induced Hyperlocomotion, Changjiu Zhao, Tao Sun, Ming Li

Department of Psychology: Faculty Publications

Clinical observations suggest that antipsychotic effect starts early and increases progressively over time. This time course of antipsychotic effect can be captured in a rat phencyclidine (PCP)-induced hyperlocomotion model, as repeated antipsychotic treatment progressively increases its inhibition of the repeated PCP-induced hyperlocomotion. Although the neural basis of acute antipsychotic action has been studied extensively, the system that mediates the potentiated effect of repeated antipsychotic treatment has not been elucidated. In the present study, we investigated the neuroanatomical basis of the potentiated action of haloperidol (HAL) and clozapine (CLZ) treatment in the repeated PCP-induced hyperlocomotion. Once daily for five consecutive days, …

2011 Annual Report Of Child Deaths In Clark County, Nevada, Nevada Institute For Children’S Research And Policy, Tara Phebus

Nevada Institute for Children's Research and Policy Reports

The primary goal of all Child Death Review Teams is to prevent future child deaths. The child death review process enables jurisdictions to come together in a collaborative, multidisciplinary forum to openly discuss detailed circumstances in an effort to gain a better understanding of child deaths. The team provides a venue for representatives from a variety of both public and private agencies as well as community organizations to share information in a confidential and non-threatening environment. The National Center for Child Death Review (hereinafter, National Center), which is supported by the Maternal and Child Health Bureau of the U.S. Department …

Immune Responses In Lactating Holstein Cows Supplemented With Cu, Mn, And Zn As Sulfates Or Methionine Hydroxy Analogue Chelates, L. M. Nemec, J. D. Richards, C. A. Atwell, D. E. Diaz, G. I. Zanton, T. F. Gressley

Physiology Collection

The aim of this study was to compare effects of inorganic sulfate versus chelated forms of supplemental Cu, Mn, and Zn on milk production, plasma and milk mineral concentrations, neutrophil activity, and antibody titer response to a model vaccination. Holstein cows (n = 25) were assigned in 2 cohorts based on calving date to a 12-wk randomized complete block design study. The first cohort consisted of 17 cows that had greater days in milk (DIM; mean of 77 DIM at the start of the trial) than the second cohort of 8 cows (32 DIM at the start of the trial). …

Sleep Hygiene And Problem Behaviors In Snoring And Non- Snoring School-Age Children, Lisa A. Witcher, David Gozal, Dennis L. Molfese, Scott M. Salathe, Karen Spruyt, Valerie Mclaughlin Crabtree

Center for Brain, Biology, and Behavior: Faculty Publications

Objectives—The effects of sleep-disordered breathing, sleep restriction, dyssomnias, and parasomnias on daytime behavior in children have been previously assessed. However, the potential relationship(s) between sleep hygiene and children’s daytime behavior remain to be explored. The primary goal of this study was to investigate the relationship between sleep hygiene and problematic behaviors in non-snoring and habitually snoring children.

Methods—Parents of 100 5- to 8-year-old children who were reported to snore “frequently” to “almost always,” and of 71 age-, gender-, and ethnicity-matched children who were reported to never snore participated in this study. As part of a larger, ongoing study, …

Microrna-34a Modulates Mdm4 Expression Via A Target Site In The Open Reading Frame, Pooja Mandke, Nicholas Wyatt, Jillian Fraser, Benjamin Bates, Steven J. Berberich, Michael P. Markey

Educator Preparation & Leadership Faculty Works

Background

MDM4, also called MDMX or HDMX in humans, is an important negative regulator of the p53 tumor suppressor. MDM4 is overexpressed in about 17% of all cancers and more frequently in some types, such as colon cancer or retinoblastoma. MDM4 is known to be post-translationally regulated by MDM2-mediated ubiquitination to decrease its protein levels in response to genotoxic stress, resulting in accumulation and activation of p53. At the transcriptional level, MDM4 gene regulation has been less clearly understood. We have reported that DNA damage triggers loss of MDM4 mRNA and a concurrent increase in p53 activity. These experiments attempt …

Inter-Method Comparison Of Us Epa Analytical Methods 507 And 508.1 To Test For Atrazine In Kentucky Drinking Water, Jonathan Suhl

Masters Theses & Specialist Projects

This study examined United States Environmental Protection Agency (US EPA) methods 507 and 508.1; analytical methods used to test drinking water for Atrazine. Additionally, this study examines the seasonal variation of Atrazine in Kentucky drinking water. Atrazine is a triazine herbicide used to control and inhibit the growth of broad leaf and grassy weeds. Atrazine’s ability to be transported to community drinking water supplies makes it a significant public health issue, as it has been linked to myriad negative health endpoints ranging from endocrine disruption to various forms of cancer, including stomach and ovarian cancer. To date, there is little …

How Can We Help Minority Nursing Students?, Nancy Schoofs

Peer Reviewed Articles

This article details the example of remediating an at-risk nursing student who failed two clinical rotations at a large university nursing program. It is a case of working with a minority student using six simulations and two clinical days in the hospital. The author describes the process of how the simulations and clinical experiences were used to assist the student in increasing her confidence and clinical abilities. In addition to the example of the student, an extensive literature review was done prior to working with the student. This aided the author not only in determining methods for assisting the student, …

Reversal By Rarα Agonist Am580 Of C-Myc-Induced Imbalance In Rarα/Rarγ Expression During Mmtv-Myc Tumorigenesis, Almudena Bosch, Silvina P. Bertran, Yongke Lu, Avalon Garcia, Alexis M. Jones, Marcia I. Dawson, Eduardo F. Farias

Pharmacology, Physiology and Toxicology

Introduction

Retinoic acid signaling plays key roles in embryonic development and in maintaining the differentiated status of adult tissues. Recently, the nuclear retinoic acid receptor (RAR) isotypes α, β and γ were found to play specific functions in the expansion and differentiation of the stem compartments of various tissues. For instance, RARγ appears to be involved in stem cell compartment expansion, while RARα and RARβ are implicated in the subsequent cell differentiation. We found that over-expressing c-Myc in normal mouse mammary epithelium and in a c-Myc-driven transgenic model of mammary cancer, disrupts the balance between RARγ and RARα/β in …

Examining The Association Between Psychiatric Illness And Suicidal Ideation In A Sample Of Treatment-Seeking Canadian Peacekeeping And Combat Veterans With Posttraumatic Stress Disorder, Don Richardson, Kate St. Cyr, Alexandra M. Mcintyre-Smith, David Haslam, Jon D. Elhai, Jitender Sareen

MacDonald Franklin OSI Research Centre

Objective:

Our study examines the association between suicidal ideation and and self-reported symptoms of posttraumatic stress disorder (PTSD), major depressive disorder (MDD), generalized anxiety disorder (GAD), and alcohol use disorder (AUD) in a sample of treatment-seeking Canadian combat and peacekeeping veterans; and identifies potential predictors of suicidal ideation.

Methods:

Actively serving Canadian Forces and Royal Canadian Mounted Police members and veterans seeking treatment at the Parkwood Hospital Operational Stress Injury Clinic (n = 250) completed measures including the Primary Care Evaluation of Mental Disorders Patient Health Questionnaire, the Alcohol Use Disorder Identification Test, and the PTSD Checklist—Military Version (PCL-M) …

Gonadotropin-Releasing Hormone Plasticity: A Comparative Perspective., T J Stevenson, T P Hahn, S A Macdougall-Shackleton, G F Ball

Brain and Mind Institute Researchers' Publications

Gonadotropin-releasing hormone 1 (GnRH1) is a key regulator of the reproductive neuroendocrine system in vertebrates. Recent developments have suggested that GnRH1 neurons exhibit far greater plasticity at the cellular and molecular levels than previously thought. Furthermore, there is growing evidence that sub-populations of GnRH1 neurons in the preoptic area are highly responsive to specific environmental and hormonal conditions. In this paper we discuss findings that reveal large variation in GnRH1 mRNA and protein expression that are regulated by social cues, photoperiod, and hormonal feedback. We draw upon studies using histochemistry and immediate early genes (e.g., c-FOS/ZENK) to illustrate that specific …

Perceptual Fluency Can Be Used As A Cue For Categorization Decisions., Sarah J Miles, John Paul Minda

Brain and Mind Institute Researchers' Publications

Learning in the prototype distortion task is thought to involve perceptual learning in which category members experience an enhanced visual response (Ashby & Maddox. Annual Review of Psychology, 56, 149-178, 2005). This response likely leads to more-efficient processing, which in turn may result in a feeling of perceptual fluency for category members. We examined the perceptual-fluency hypothesis by manipulating fluency independently from category membership. We predicted that when perceptual fluency was induced using subliminal priming, this fluency would be misattributed to category membership and would affect categorization decisions. In a prototype distortion task, the participants were more likely to judge …