Medicine and Health Sciences Commons^™

Detection Of Protein By Microdrop Analysis, Yogeshkumar Radadiya

All Capstone Projects

Analysis of protein depends particularly on protein concentration. Protein concentration measurement is the most important part in the research work to conduct protein-related studies. Although there are many methods available for this purpose, each method has certain limitations. The aim of the experiment is to develop either new or modified analytical method for the analysis and detection of protein using newly introduced micro-plate reader equipped with Take-3 microplate with the help of Gen-5 software. An ideal assay should be simple and easy to carry out. Another aspect to be taken into consideration are low inference, stability of measured components and …

A Transylvania Medical Graduate Searches For His Sons After A Civil War Battle, Charles T. Ambrose

Microbiology, Immunology, and Molecular Genetics Faculty Publications

No abstract provided.

Transjugular Intrahepatic Portosystemic Shunt (Tips); Review Of Initial Experience At Aga Khan University Hospital, Rana Shoaib Hamid, Tanveer-Ul-Haq, Muhammad Azeemuddin, Zafar Sajjad, Ishtiaq Chishti, Basit Salam

Department of Radiology

OBJECTIVE: To retrospectively assess the therapeutic effectiveness and safety of transjugular intrahepafic portosystemic shunt (TIPS) in patients with portal hypertension related complications.

METHODS: Over a period of 7.5 years 19 patients (10 males and 9 females, age range 25-69 years) were referred for TIPS at our radiology department. Thirteen patients suffered from liver cirrhosis while 6 had Budd Chiari syndrome. All patients were evaluated with colour doppler ultrasonography and cross sectional imaging. Shunt procedures were performed under sedation or general anaesthesia through internal jugular approach. Metallic self expandable uncovered stents were placed in 15 patients and covered stent in 1 …

Goldston Syndrome, Zainab Hussain, Imrana Masroor, Qurrat-Ul-Ain Haider, Tariq Alam

Department of Radiology

Goldston syndrome is a rare entity describing the association of polycystic kidneys and Dandy Walker malformation with or without hepatic fibrosis. A 28 years old pregnant woman came to radiology department for the first antenatal scan. Ultrasound showed a single fetus corresponding to 27 week of gestation. The fetal brain revealed deficient vermis with a posterior fossa cyst communicating with the 4th ventricle suggestive of Dandy-Walker malformation (DWM). The kidneys were enlarged, and echogenic containing small cysts with associated amniotic fluid index of 5 cm suggestive of Oligohydramnios. A diagnosis of Goldston syndrome was suggested. These findings were confirmed on …

Thermoregulatory And Exercise Performance : Responses To Cooling With Ice Slurry Ingestion, Rodney Siegel

Theses: Doctorates and Masters

The rise in body core, skin and muscle temperatures associated with exercise
in hot environments (~30°C and above) is known to impair performance over a
variety of exercise modes and durations. Precooling has become a popular strategy to
combat this impairment, as evidence has shown it to be an effective method for
lowering pre-exercise core temperature, increasing heat storage capacity and
improving exercise performance in the heat. To date, the majority of precooling
manoeuvres are achieved via external means, such as cold water immersion and the
application of cooling garments; methods which have been criticised for their lack of
practicality …

Primary Hyperparathyrodism In Pregnancy And Review Of Literature, Tahira Naru, Rozilla Sadia Khan, Mohammed Ataullah Khan

Department of Obstetrics & Gynaecology

hyperparathyroidism (PHPT) is rare condition during pregnancy; however, it can carry significant risks to both mother and foetus during and after the pregnancy. The definitive treatment is parathyroidectomy undertaken preferably in the second or early third trimester. A case of asymptomatic PHPT diagnosed and treated by parathyroidectomy in the third trimester is described.

The Hypothesis Of Apraxia Of Speech In Children With Autism Spectrum Disorder, Lawrence D. Shriberg, Rhea Paul, Lois M. Black, Jan P. Van Santen

Communication Disorders Faculty Publications

In a sample of 46 children aged 4-7 years with Autism Spectrum Disorder (ASD) and intelligible speech, there was no statistical support for the hypothesis of concomitant Childhood Apraxia of Speech (CAS). Perceptual and acoustic measures of participants' speech, prosody, and voice were compared with data from 40 typically-developing children, 13 preschool children with Speech Delay, and 15 participants aged 5-49 years with CAS in neurogenetic disorders. Speech Delay and Speech Errors, respectively, were modestly and substantially more prevalent in participants with ASD than reported population estimates. Double dissociations in speech, prosody, and voice impairments in ASD were interpreted as …

Bionic Technologies Transforming The Science Of Healthcare Delivery, Scott A. Waldman , M.D., Ph.D, Andre Terzic , M.D., Ph.D

Department of Pharmacology and Experimental Therapeutics Faculty Papers

Individualization of patient care is creating an envisioned future in which practitioners wield a new doctor’s bag deploying individual molecular, genetic, cellular, and systems profiles.  These emerging tools are refining traditional paradigms of disease palliation into nuanced patient management algorithms employing prognostic risk stratification, therapeutic response prediction, and adverse event avoidance. Advancing technologies are enabling a shift to more proximal nodes along the continuum of pathobiology. Innovations in biomarker platforms, genomic profiling, and molecular imaging reveal the earliest stages of pathophysiology, limiting systems disruption to cells and tissues while preserving integrated organ function, enabling risk mitigation and disease prevention.  At …

Role Of Breastfeeding And Current Lifestyle On Maternal Long-Term Weight Change, Denise R. Hinds

Loma Linda University Electronic Theses, Dissertations & Projects

Background: Evidence suggests that prolonged and exclusive breastfeeding is associated with health benefits to children and nursing mothers beyond the period of breastfeeding. Studies that support the role of breastfeeding in postpartum weight change primarily focused on short-term weight change (within 12 to 24 months postpartum) (Binns et al. 2003; Lederman 2004; Johnson et al. 2006), while few focused on the association between breastfeeding and long-term weight change (5 years or more postpartum) (Rooney, Schauberger et al. 2005).

Purpose: This study investigated the role of breastfeeding in long-term weight change, specifically 1) whether breastfeeding protects against weight gain, and 2) …

Oncologic Efficacy Is Not Compromised, And May Be Improved With Minimally Invasive Esophagectomy., Adam C Berger, Aaron Bloomenthal, Benny Weksler, Nathaniel Evans, Karen A Chojnacki, Charles J Yeo, Ernest L Rosato

Department of Surgery Faculty Papers

BACKGROUND: Major morbidity and mortality rates continue to be high in large series of transthoracic esophagectomies. Minimally invasive approaches are being increasingly used. We compare our growing series of minimally invasive (combined thoracoscopic and laparoscopic) esophagectomies (MIEs) with a series of open transthoracic esophagectomies.

STUDY DESIGN: We identified 65 patients who underwent an MIE with thoracoscopy/laparotomy (n = 11), Ivor Lewis (n = 2), or 3-hole approach (n = 52). These patients were compared with 53 patients who underwent open Ivor-Lewis esophagectomy (n = 15) or 3-hole esophagectomy (n = 38) over the past 10 years.

RESULTS: The MIE and …

Potent Inhibition Of Heterotopic Ossification By Nuclear Retinoic Acid Receptor-Γ Agonists., Kengo Shimono, Wei-En Tung, Christine Macolino, Amber Hsu-Tsai Chi, Johanna H Didizian, Christina Mundy, Roshantha A Chandraratna, Yuji Mishina, Motomi Enomoto-Iwamoto, Maurizio Pacifici, Masahiro Iwamoto

Department of Orthopaedic Surgery Faculty Papers

Heterotopic ossification consists of ectopic bone formation within soft tissues after surgery or trauma. It can have debilitating consequences, but there is no definitive cure. Here we show that heterotopic ossification was essentially prevented in mice receiving a nuclear retinoic acid receptor-γ (RAR-γ) agonist. Side effects were minimal, and there was no significant rebound effect. To uncover the mechanisms of these responses, we treated mouse mesenchymal stem cells with an RAR-γ agonist and transplanted them into nude mice. Whereas control cells formed ectopic bone masses, cells that had been pretreated with the RAR-γ agonist did not, suggesting that they had …

Characterization Of Amino Acid Residues Integral To Neuronal Binding Of Amyloid Beta Protein In Alzheimer’S Disease, Nicole C. Olson

Chemistry and Biochemistry

Purpose:  Alzheimer’s Disease is a neurodegenerative disease resulting from over-production and neuronal accumulation of amyloid-beta proteins (Aβ40/Aβ42). The glycine residue at position 33 and histidine residues at positions 13 and 14 are involved with binding and internalization of these proteins, actions potentially inhibited by substituting or sterically hindering these residues with an antibody specific to positions 2-11 (IgG-4.1). Rat pheochromocytoma (PC12) cells differentiated with nerve growth factor were used as a neuronal model to determine whether substitution and/or antibody block amyloid-beta’s neuronal interactions.

Methods:  PC12 cells were incubated with fluorescein-labeled-amyloid-beta-40 (F-Aβ40) or substituted F-Aβ40 derivatives (F-Aβ40-H13,14G, F-Aβ40-H13,14G;G33A), with or without …

Longitudinal Assessment Of Emotional Intelligence In Doctor Of Physical Therapy Students, Erika Lewis

Internet Journal of Allied Health Sciences and Practice

Emotional intelligence has been shown to predict clinical performance in other medical fields and may be a predictor for clinical performance in physical therapy students. Longitudinal assessment of emotional intelligence of Doctor of Physical Therapy students was obtained yearly (three times) beginning in the first year. In addition, the relationship between emotional intelligence and performance on the National Physical Therapy Examination (NPTE) and clinical performance (using the Clinical Performance Instrument (CPI)) was examined. Participants. Graduate physical therapy students (n = 260; 218 women and 42 men) between the ages of 20 and 35 from four schools participated. Methods. Mayer-Salovey-Caruso Emotional …

Effect Of Light-Cure Initiation Time On Polymerization And Orthodontic Bond Strength With A Resin-Modified Glass-Ionomer, Jess Thomas

Master's Theses (2009 -)

Introduction: The polymerization and acid<–>base reactions in resin<–>modified glass<–>ionomers (RMGI) are thought to compete with and inhibit one another. The objective of this study was to examine the effect of visible light<–>cure (VLC) delay on the polymerization efficiency and orthodontic bond strength of a dual<–>cured RMGI.

Methods: An RMGI light<–>cured immediately, 2.5, 5, or 10 minutes after mixing comprised the experimental groups. Isothermal and dynamic temperature scan differential scanning calorimetry (DSC) analysis of the RMGI was performed to determine extents of VLC polymerization and acid<–>base reaction exotherms. Human premolars (n = 18/group) were …

Agenesis Of Maxillary Lateral Incisors In Orthodontic Patients And The Relation To Overall Tooth Size, Jane Wright

Master's Theses (2009 -)

The maxillary lateral incisor is the second most frequently missing tooth in the dental arch with clinical management requiring a complex and multidisciplinary treatment approach. It has been suspected that teeth could be smaller in the maxillary or mandibular arches in patients experiencing agenesis of the maxillary lateral incisor, making ideal occlusal relationships and space creation for restoration of the lateral incisor difficult to obtain. The purpose of this study is to determine if a tooth size discrepancy exists in orthodontic patients with agenesis of the maxillary lateral incisor(s).

Forty sets of dental casts from Caucasian orthodontic patients (19 male …

The Effects Of Different Surface Treatments On The Shear Bond Strength Of Composite Resin To Machined Titanium, Mohammad Sadeq Aljadi

Master's Theses (2009 -)

Purpose: The purpose of this study was to evaluate the shear bond strength between machined titanium and composite resin using different surface treatments.

Materials and Methods: Titanium (Ti-6Al-4V) specimens were ground with 600 grit SiC paper and randomly divided into 6 groups (n=20/group). Group #1 (Control): samples were sandblasted with 110 µm Al₂O₃ for 10 sec. Group #2 (Rocatec): samples were treated with the Rocatec system following the manufacturer's directions but the silanization step was eliminated. Group #3 (Silano Pen): samples were treated with the Silano Pen system. Group #4 (H₂SO₄ etched): samples were …

Friction Testing Of A New Ligature, Alison Mantel

Master's Theses (2009 -)

Objective: To determine if American Orthodontics' (AO) new, experimental ligature demonstrates less friction in vitro when compared to four other ligatures on the market.

Methods: Four brackets were mounted on a custom metal fixture allowing an 0.018-in stainless steel wire attached to an opposite fixture with one bracket to be passively centered in the bracket slot. The wire was ligated to the bracket using one of five types of ligatures including the low friction test ligatures (AO), conventional ligatures (AO), Sili–TiesTM, Silicone Infused Ties (GAC), Synergy® Low-Friction Ligatures (RMO), and SuperSlick ligatures (TP Orthodontics). Resistance to sliding was measured over …

Metallurgical Characterization Of New Palladium-Containing Cocr And Nicr Alloys, Raghav Puri

Master's Theses (2009 -)

Recently introduced to the market has been an entirely new subclass of casting alloy composition whereby palladium (~25 wt%) is added to traditional base metal alloys such as CoCr and NiCr.

Objectives: The purpose of this study was to evaluate the microstructure and Vickers hardness of two new CoPdCr and one new NiPdCr alloy and compare them to traditional CoCr and NiCr alloys.

Methods: The casting alloys investigated were: CoPdCr-A (Noble Crown NF, The Argen Corporation), CoPdCr-I (Callisto CP+, Ivoclar Vivadent), NiPdCr (Noble Crown, Argen), CoCr (Argeloy N.P. Special, Argen), and NiCr (Argeloy N.P. Star, Argen). As- cast cylindrical alloy …

Effects Of Rapid Maxillary Expansion On Upper Airway; A 3 Dimensional Cephalometric Analysis, Yoon Hwan Chang

Master's Theses (2009 -)

The purpose of this study was to use cone-beam computed tomography (CBCT) to assess changes in the volume and cross sectional areas of the upper airway in children with maxillary constriction treated by rapid maxillary expansion (RME).

The study group consisted of 5 males and 9 females with mean age of 12.93 years with posterior cross bite and constricted maxilla who were treated with hyrax expander as part of their comprehensive orthodontic treatment. Pre and post RME CBCT scans were analyzed with 3D Dolphin 11.0 software to measure the retropalatal (RP) and retroglossal (RG) airway changes including volume and cross …

Marginal Ridge Thickness (Mrt) Of Maxillary Incisors, Mark Foster

Master's Theses (2009 -)

Aim: To determine the marginal ridge thickness (MRT) of maxillary incisors in orthodontic patients and the possible correlation between MRT and Bolton Index.

Methods: 120 pre<–>orthodontic treatment dental casts were collected, following the inclusion criteria: Quality pretreatment upper and lower casts, age 10<–>16, full permanent dentition (except 2nd and 3rd molars), no obvious wear/attrition/abrasion, no proximal restorations or crowns. For each cast, the mesio (M<–>D)distal (M<–>D) widths of the upper and lower teeth from left 1st molar to right 1st molar were measured to calculate Bolton Index. The MRTs of the maxillary incisors were measured in …

Eating For Two: A Study Of The Effects Of Obesity Upon The Health Of Pregnant Women And Their Infants, Michael Cochran-Boucher

Inquiry Journal 2011

No abstract provided.

Creative And Stylistic Devices Employed By Children During A Storybook Narrative Task: A Cross-Cultural Study, Brenda K. Gorman, Christine Fiestas, Elizabeth Peña, Maya Reynolds Clark

Speech Pathology and Audiology Faculty Research and Publications

Purpose: The purpose of this study was to analyze the effects of culture on the creative and stylistic features children employ when producing narratives based on wordless picture books.

Method: Participants included 60 first- and second-grade African American, Latino American, and Caucasian children. A subset of narratives based on wordless picture books collected as part of a larger study was coded and analyzed for the following creative and stylistic conventions: organizational style (topic centered, linear, cyclical), dialogue (direct, indirect), reference to character relationships (nature, naming, conduct), embellishment (fantasy, suspense, conflict), and paralinguistic devices (expressive sounds, exclamatory utterances).

Results …

Comparisons Of Three Analytical Techniques For Measuring Absolute And Relative Temperature Changes During Ultrasound Treatment, Michael G. Miller

Dissertations

Ultrasound is a thermal modality that utilizes acoustic energy to promote heating. While there are many factors that affect heating of body tissues, the effects of skinfold thickness and skin temperature upon ultrasound heating have not been studied extensively. In addition, while temperature typically follows a linear trend, past research typically uses ANOVA or regression analysis to examine this relationship; but these models examine the within-subject effects (time) and between effects (usually groups) at the group level, not the individual level. Therefore, the purposes of this study are to determine if skin temperature and skinfold thickness are predictors for intramuscular …

An In Vitro Evaluation Of Microleakage In Class V Preparations Restored With Hybrid Versus Silorane Composites., Fahad Umer, Farah Naz Farid, Farhan Raza Khan

Section of Dental-Oral Maxillofacial Surgery

Aim: To compare the difference in microleakage values using conventional and new low-shrink Silorane composites using the dye penetration method. Background: With improvement in adhesive technology, problems associated with composites have been largely overcome. However, microleakage due to polymerization shrinkage still remains a challenge. New Silorane-based composites are alleged to have less than 1% polymerization shrinkage, which would potentially decrease the microleakage, and hence, resolve this longstanding issue. Materials And Methods: The teeth were randomly divided into three groups (A,B, and C). Group A was restored with conventional composite (Valux() Plus), with a total-etch protocol, Group B was restored with …

Spatial Discounting Of Food And Social Rewards In Guppies (Poecilia Reticulata), Nelly Mühlhoff, Jeffrey R. Stevens, Simon M. Reader

Department of Psychology: Faculty Publications

In temporal discounting, animals trade off the time to obtain a reward against the quality of a reward, choosing between a smaller reward available sooner versus a larger reward available later. Similar discounting can apply over space, when animals choose between smaller and closer versus larger and more distant rewards. Most studies of temporal and spatial discounting in non-human animals use food as the reward, and it is not established whether animals trade off other preferred stimuli in similar ways. Here, we offered female guppies (Poecilia reticulata) a spatial discounting task in which we measured preferences for a larger reward …

Content Validation Of A Standardized Language Diagnosis By Certified Specialists In Gerontological Nutrition, Paula K. Ritter-Gooder, Nancy M. Lewis, Kent M. Eskridge

Department of Nutrition and Health Sciences: Faculty Publications

Validation of the nutrition standardized language assures the language is accurate for use in practice, policy, and research, but few validation studies have been reported. The purpose of this descriptive study was to validate content of all components of the nutrition diagnostic term involuntary weight loss using experts providing care for older adults in health care settings. A Nutrition Diagnosis Validation Instrument was developed that contained the definition, etiologies, and signs and symptoms of the diagnosis plus items added from literature review. Questions on clarity and completeness of the language were included. The Nutrition Diagnosis Validation Instrument used a Likert-type …

Fit Into College: A Program To Improve Physical Activity And Dietary Intake Lifestyles Among College Students, Robert V. Topp, Jean S. Edward, S. Lee Ridner, Dean E. Jacks, Karen Newton, Patricia Keiffner, Deborah Woodall, Kathleen P. Conte

College of Nursing Faculty Research and Publications

The purpose of this study was to determine whether a 10-week program could improve physical activity, physical fitness, body weight, dietary intake, and perceptions of exercise and diet among college 30 healthy college freshmen. Outcomes were measured at baseline, and following the 10-week program. The weekly sessions incorporated constructs of the Transtheoretical Model of Health Behavior Change and were administered by fitness interns who were junior or senior college students enrolled in health-related majors. The participants presented with low physical activity, physical fitness, and poor dietary intake, and 50% were overweight/obese (BMI > 25). Participants demonstrated gains in their physical fitness …

A Comparison Of Antibiotic Resistant Escherichia Coli To The Wild Type Strain, Matthew Thomas Schreiner

Senior Honors Theses

Antibiotic resistance is a growing problem that has rendered many antibiotics ineffective. Bacteria can gain resistance through spontaneous mutations and horizontal gene transfer. A better understanding of the overarching effects of antibiotic resistance on resistant strains is necessary when considering possible solutions to this issue. Tetracycline and doxycycline resistant strains of E. coli were compared to a wild type K-12 strain to determine if resistance engenders any fitness costs. The different strains were compared using antibiotic sensitivity tests, growth rate analysis and metabolic tests. The resistant strains actually grew at a slightly faster rate than the wild type strain. However, …

The Adverse Effects Of The Cardiopulmonary Bypass Machine, Shannon Renee Steidl

Senior Honors Theses

Throughout the United States, the use of the cardiopulmonary bypass (CPB) machine during cardiac surgery has become a widely employed practice. Although this machine has simplified cardiac surgery by allowing the heart to be stopped, the technology also causes adverse effects on a patient’s body and cognitive functions. These include complications of the inflammatory system, heart, lungs, kidneys, and brain. Using the CPB can not only cause physical harm, but it can also lead to cognitive decline that can affect the patient’s quality of life. Studying the CPB in terms of its adverse effects is imperative to making any effort …

Why Not Just Google It? An Assessment Of Information Literacy Skills In A Biomedical Science Curriculum, Karl Kingsley, Gillian M. Galbraith, M. Herring, Eva Stowers, Tanis Stewart, Karla V. Kingsley

Library Faculty Research

Background: Few issues in higher education are as fundamental as the ability to search for, evaluate, and synthesize information. The need to develop information literacy, the process of finding, retrieving, organizing, and evaluating the ever-expanding collection of online information, has precipitated the need for training in skill-based competencies in higher education, as well as medical and dental education.

Methods: The current study evaluated the information literacy skills of first-year dental students, consisting of two, consecutive dental student cohorts (n = 160). An assignment designed to evaluate information literacy skills was conducted. In addition, a survey of student online search engine …