Medicine and Health Sciences Commons^™

Samoan Speech Audiometry: Developing Word Recognition Materials For Native Speakers Of Samoan, Emma Lilian Kruger

Theses and Dissertations

Hearing can be evaluated through the presentation of tones or speech. Speech audiometry determines an individual's speech recognition threshold and word recognition score. Traditionally these materials were developed using familiar, frequently used, monosyllabic words. Currently, there are various types of word recognition materials including those which use word lists, short half-lists, and materials which use sentences level stimuli with competing noise. Word recognition materials were first developed in Standard American English; today, materials are now readily available in many other languages. When possible, word recognition materials are developed digitally to standardize their presentation. Currently, no recorded word recognition materials are …

Rural Versus Urban: Tennessee Health Administrators' Strategies On Recruitment And Retention For Allied Health Professionals., Derek Ray Slagle

Electronic Theses and Dissertations

There is a growing interest in understanding recruitment, retention, and turnover of allied health professionals considering employment trends and workforce mobility, an increased need to understand the healthcare delivery system, and the dynamic nature of the allied health workforce especially for rural areas. A survey was sent to allied health administrators across a variety of allied health disciplines from the state of Tennessee hospitals in order to gauge opinions on retention and recruitment strategies. Overall successful strategies for recruitment and retention of allied health professionals were reported as well as differences between urban and rural areas, differences among allied health …

Narcissism And Binge Drinking: Exploring The Role Of Overconfidence And Confidence-Based Risk-Taking., Alicia M. Wood

Electronic Theses and Dissertations

Binge drinking (BD) entails excessive alcohol intake in a short time period. Despite numerous negative outcomes associated with BD and efforts to curtail it, rates remain steady. Thus, it is important to identify "who" binge drinks and "why" it occurs. Drawing from past research, I sought to replicate the link between trait narcissism and BD; moreover, I examined if overconfidence and confidence-based risk-taking assessed via the Georgia Gambling Task (GGT), explained why they did so. The results generally supported my hypotheses. As expected, narcissism related to poor GGT performance and high levels of BD; likewise, poor GGT performance related to …

Synthesis Of A Water Soluble Resveratrol Derivative As A Potential Anti-Cancer Drug., Augustine Essel

Electronic Theses and Dissertations

Research on development of water soluble anti-cancer drugs is one of the great challenges of modern medicinal chemistry. Resveratrol (Res) is one of the many phytoalexins producing stilbenoids present in several medicinal plants, grape skin, peanuts, and red wine. It has been found to exhibit anti-cancer, anti-inflammatory, and anti-oxidant properties. Water solubility and bioavailability are some of the setbacks of this interesting compound. In view of this, effort has been made to synthesize amino acid derivative of resveratrol to improve its bioavailability and solubility in water. Methyl 4-{-[(1E)-2-(3, 5-dihydroxyphenyl)-ethenyl] - phenoxy} butyrate (7), a novel ester intermediate, has been synthesized …

Adam10 Is A Critical Regulator Of B Cell Development, Antibody Production, And Myeloid-Derived Suppressor Cell Expansion: Effects Of B Cell-Specific Adam10 Deletion And Overexpression In Vivo., David Gibb

Theses and Dissertations

Proteolytic processing of transmembrane receptors and ligands can have dramatic effects on cell signaling and subsequent cellular responses. Previous studies demonstrated that a disintegrin and metalloproteinase 10 (ADAM10) may cleave numerous B cell-expressed receptors, including the low affinity IgE receptor (CD23). However, lethality of ADAM10-deficient embryos has limited examination of these cleavage events in lymphocytes. To investigate their role in B cell development and function, we generated B cell-specific ADAM10 knockout mice. Intriguingly, deletion prevented development of the entire marginal zone B cell (MZB) lineage. Further analysis revealed that ADAM10 is required for S2 cleavage of the Notch2 receptor and …

Cortisol Exerts Bi-Phasic Regulation Of Inflammation In Humans, Mark P. Yeager, Patricia Pioli, Paul M. Guyre

Dartmouth Scholarship

Natural and synthetic glucocorticoids (GCs) have been used for decades to suppress inflammation. In this paper, we re-examine the role of the endogenous GC, cortisol, as a primary homeostatic regulator of the human inflammatory response to injury. Our data show that cortisol regulation of innate immunity can be both pro-inflammatory and anti-inflammatory. Using a human model of in vivo cortisol depletion, we first show that baseline (diurnal) cortisol concentrations do not exert an anti-inflammatory effect. This is the first clue that cortisol regulation of inflammation is not represented by a linear dose-response relationship. We next show in surgical patients that …

The P Wave: Indicator Of Atrial Enlargement, Patrick Loftis

Physician Assistant Studies Faculty Research and Publications

No abstract provided.

A Monocular Marker-Free Gait Measurement System, Jane Courtney, Annraoi M. De Paor

Articles

This paper presents a new, user-friendly, portable motion capture and gait analysis system for capturing and analyzing human gait, designed as a telemedicine tool to monitor remotely the progress of patients through treatment. The system requires minimal user input and simple single-camera filming (which can be acquired from a basic webcam) making it very accessible to nontechnical, nonclinical personnel. This system can allow gait studies to acquire a much larger data set and allow trained gait analysts to focus their skills on the interpretation phase of gait analysis. The design uses a novel motion capture method derived from spatiotemporal segmentation …

Acaricidal Activity Of Eugenol Based Compounds Against Scabies Mites, Cielo Pasay, Kate Mounsey, Graeme Stevenson, Rohan Davis, Larry G. Arlian, Marjorie Morgan, Diann Vyszenski-Moher, Kathy Andrews, James Mccarthy

Biological Sciences Faculty Publications

Background

Human scabies is a debilitating skin disease caused by the “itch mite” Sarcoptes scabiei. Ordinary scabies is commonly treated with topical creams such as permethrin, while crusted scabies is treated with topical creams in combination with oral ivermectin. Recent reports of acaricide tolerance in scabies endemic communities in Northern Australia have prompted efforts to better understand resistance mechanisms and to identify potential new acaricides. In this study, we screened three essential oils and four pure compounds based on eugenol for acaricidal properties.

Methodology/Principal Findings

Mirnas In Newt Lens Regeneration: Specific Control Of Proliferation And Evidence For Mirna Networking, Kenta Nakamura, Nobuyasu Maki, Albert Trinh, Heidi W. Trask, Jiang Gui, Craig R. Tomlinson, Panagiotis A. Tsonis

Dartmouth Scholarship

Background:

Lens regeneration in adult newts occurs via transdifferentiation of the pigment epithelial cells (PECs) of the dorsal iris. The same source of cells from the ventral iris is not able to undergo this process. In an attempt to understand this restriction we have studied in the past expression patterns of miRNAs. Among several miRNAs we have found that mir-148 shows an up-regulation in the ventral iris, while members of the let-7 family showed down-regulation in dorsal iris during dedifferentiation.

Methodology/Principal Findings:

Brain Mapping Of The Mismatch Negativity And The P300 Response In Speech And Nonspeech Stimulus Processing, Skylee Simmons Neff

Theses and Dissertations

Previous studies have found that behavioral and P300 responses to speech are influenced by linguistic cues in the stimuli. Research has found conflicting data regarding the influence of phonemic characteristics of stimuli in the mismatch negativity (MMN) response. The current investigation is a replication of the study designed by Tampas et al. (2005), which studied the effects of linguistic cues on the MMN response. This current study was designed to determine whether the MMN response is influenced by phonetic or purely acoustic stimuli, and to expand our knowledge of the scalp distribution of processing responses to within- and across-category speech …

Primary Human Mammary Epithelial Cells Endocytose Hiv-1 And Facilitate Viral Infection Of Cd4+ T Lymphocytes, Stephanie M. Dorosko, Ruth I. Connor

Dartmouth Scholarship

The contribution of mammary epithelial cells (MEC) to human immunodeficiency virus type 1 (HIV-1) in breast milk remains largely unknown. While breast milk contains CD4(+) cells throughout the breast-feeding period, it is not known whether MEC directly support HIV-1 infection or facilitate infection of CD4(+) cells in the breast compartment. This study evaluated primary human MEC for direct infection with HIV-1 and for indirect transfer of infection to CD4(+) target cells. Primary human MEC were isolated and assessed for expression of HIV-1 receptors. MEC were exposed to CCR5-, CXCR4- and dual-tropic strains of HIV-1 and evaluated for viral reverse transcription …

Health Care In Aymara Women In The North Of Chile, Rossana Testa Phd, Academic Director

Fostering Multicultural Competence and Global Justice: an SIT Symposium

Background: In Chile the most important indigenous population lives in a determined territory, we found Mapuche people in the south and the Aymara people in the north of the country. The majority of the indigenous people live in the urban zones. However, the indigenous people that live at the rural areas are who conserve their traditional medicine practices.

The region of Arica and Parinacota is the northern region of Chile and has the largest concentration of the Aymara indigenous group in all of Chile.

At the rural areas are the women whom most attended at the public health system, especially …

Strengthening Civil Society And Community Capacity To Respond To Hiv/Aids, Blanka Homolova Ma, Senior Hiv/Aids Specialist, Jennifer Whatley Ma, Senior Technical Specialist For Civil Society And Governance Programs

Fostering Multicultural Competence and Global Justice: an SIT Symposium

The Global Fund and PEFPAR have increased resources for preventing, treating, and caring for HIV/AIDS and dramatically changed the ways in which the HIV/AIDS epidemic is addressed internationally. While attention often focuses on the roles of government and health workers, national civil society organizations (CSOs) and local communities are gaining recognition and key roles to play in responding to HIV/AIDS in many countries. For CSOs and communities, however, accessing Global Fund and PEPFAR resources can be challenging given these mechanisms’ technical, financial, and programmatic requirements. Insufficient capacity of potential recipients to manage the funds presents a challenge for donors as …

The Feminization Of Hiv: Patriarchy As A Threat To Public Health In Ecuador – A Trans-Feminist Perspective, Fabián Espinosa Ma, Academic Director

Fostering Multicultural Competence and Global Justice: an SIT Symposium

This article attempts to analyze male sexual conduct in Ecuador and its determining role in the feminization of HIV. Male promiscuity, not only tolerated but celebrated by sexist discourse, severely impairs public health initiatives and remains one of the critical issues for both individuals and collectives struggling for structural changes in sexual politics. The exercise of citizenships, sustained by a new constitutional framework, embraces the principle of “subversion from within”, the only significant way of challenging heterosexist imaginaries and practices. This type of activism focuses on the formal and normative political dimension of sex and gender, but also and most …

Comparative Experiential Education In Global Health For Undergraduates: A Platform For Careers And Lifelong Learning In Public Health, Lois Mccloskey Drph, Mph, Onsultant, Founding Program Director, Health And Community, Christopher J. Colvin Phd, Mph, Program Director, Faculty And Country Coordinator, South Africa, Health And Community

Fostering Multicultural Competence and Global Justice: an SIT Symposium

In 2005, the International Honors Program, a longstanding organization that runs comparative study abroad programs, launched its “Health and Community” (HC) Program. The program is offered to upper-level undergraduates from across the US and from a wide range of disciplinary backgrounds. It travels to four countries in one semester and uses lectures, site visits, case study research and homestay experiences to engage students in critical and comparative thinking about health and illness at both the global and community levels. The interdisciplinary curriculum combines the perspectives of political economy, medical anthropology, and public health and each country program focuses learning around …

A Team Public Health Research Project For First-Year Pharmacy Students To Apply Content From Didactic Courses, David Fuentes, Nancy Deguire, Rajul A. Patel, Eric G. Boyce

School of Pharmacy Faculty Articles

Objectives. To implement and assess a first-year pharmacy student group research project that provided practical hands-on application and reinforced the curricula of concurrent didactic courses.

Design. Groups of 6 to 7 students chose a public health topic based on the Healthy People 2010 Priority Areas and created a survey instrument. Faculty facilitated mock institutional review board (IRB) review sessions which provided teams with ongoing feedback and refinement recommendations before each team administered their survey instrument to a predefined population. Data analysis, formal written reports, and oral presentations were presented to peers and project faculty members.

Assessment. Teams complied with the …

Weighing In: A Critical Analysis Of New York City’S Calorie Labeling Law, Josephine Barnett

Publications and Research

The ‘obesity’ epidemic has The health of New York City Residents has been a significant concern of public health officials with the rates of obesity and diabetes ranking eighth of all cities nationally. The New York City (NYC) Board of Health laid the foundation and influenced the legislative efforts of means to address the ‘obesity-diabetes’ epidemic for public health officials consider this to be one of the major health concerns among Americans and particularly NYC residents. The major initiatives implemented by NYC official include: (1) a ban on trans-fat (2) a city registry of those with diabetes, and (3) menu-labeling. …

Radially Aligned, Electrospun Nanofibers As Dural Substitutes For Wound Closure And Tissue Regeneration Applications, Jingwei Xie, Matthew R. Macewan, Wilson Z. Ray, Wenying Liu, Daku Y. Siewe, Younan Xia

MIIR Faculty Research

This paper reports the fabrication of scaffolds consisting of radially aligned poly(ε-caprolactone) nanofibers by utilizing a collector composed of a central point electrode and a peripheral ring electrode. This novel class of scaffolds was able to present nanoscale topographic cues to cultured cells, directing and enhancing their migration from the periphery to the center. We also established that such scaffolds could induce faster cellular migration and population than nonwoven mats consisting of random nanofibers. Dural fibroblast cells cultured on these two types of scaffolds were found to express type I collagen, the main extracellular matrix component in dural mater. The …

Warren County, Kentucky Hazardous Materials Commodity Flow Analysis, Dr. Ritchie D. Taylor, Dr. Vijay Golla, Jacqueline Brown, Shailesh Advani, Pragati Gole, Rasmi Nair, Bob Myatt

Public Health Faculty Publications

This report presents the results of a Commodity Flow Analysis of Hazardous Materials for I-65 and the William H. Natcher Parkway conducted by Western Kentucky University in partnership with the Warren County (Kentucky) Local Emergency Planning Committee (LEPC). Kentucky counties within the study area include Warren, Simpson, Edmonson, Butler, Barren, and Hart. Figure 1 shows the sections of I-65 and Natcher Parkway that run through these counties. The purpose of our report is to give information on patterns of hazardous materials being transported along I-65 and Natcher Parkway as observed from May 24th 2010 to June 18th 2010. A secondary …

Formation Of Complex And Unstable Chromosomal Translocations In Yeast, Kristina H. Schmidt, Emilie Viebranz, Lillian Doerfler, Christina Lester, Aaron Rubenstein

Molecular Biosciences Faculty Publications

Genome instability, associated with chromosome breakage syndromes and most human cancers, is still poorly understood. In the yeast Saccharomyces cerevisiae, numerous genes with roles in the preservation of genome integrity have been identified. DNA-damage-checkpoint-deficient yeast cells that lack Sgs1, a RecQ-like DNA helicase related to the human Bloom's-syndrome-associated helicase BLM, show an increased rate of genome instability, and we have previously shown that they accumulate recurring chromosomal translocations between three similar genes, CAN1, LYP1 and ALP1. Here, the chromosomal location, copy number and sequence similarity of the translocation targets ALP1 and LYP1 were altered to gain insight into the …

The Staphylococcus-Specific Gene Rsr Represses Agr And Virulence In Staphylococcus Aureus, Sandeep Tamber, Dindo Reyes, Niles P. Donegan, Joseph D. Schwartzman, Ambrose L. Cheung, Guido Memmi

Dartmouth Scholarship

The expression of virulence factors in Staphylococcus aureus is tightly coordinated by a vast network of regulatory molecules. In this report, we characterize a genetic locus unique to staphylococci called rsr that has a role in repressing two key virulence regulators, sarR and agr. Using strain SH1000, we showed that the transcription of virulence effectors, such as hla, sspA, and spa, is altered in an rsr mutant in a way consistent with agr upregulation. Analysis of RNAIII expression of the agr locus in rsr and rsr-sarR mutants indicated that rsr likely contributes to agr expression independently …

S-Glutathionylation Activates Stim1 And Alters Mitochondrial Homeostasis., Brian J Hawkins, Krishna M Irrinki, Karthik Mallilankaraman, Yu-Chin Lien, Youjun Wang, Cunnigaiper D Bhanumathy, Ramasamy Subbiah, Michael F Ritchie, Jonathan Soboloff, Yoshihiro Baba, Tomohiro Kurosaki, Suresh K Joseph, Donald L Gill, Muniswamy Madesh

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Oxidant stress influences many cellular processes, including cell growth, differentiation, and cell death. A well-recognized link between these processes and oxidant stress is via alterations in Ca(2+) signaling. However, precisely how oxidants influence Ca(2+) signaling remains unclear. Oxidant stress led to a phenotypic shift in Ca(2+) mobilization from an oscillatory to a sustained elevated pattern via calcium release-activated calcium (CRAC)-mediated capacitive Ca(2+) entry, and stromal interaction molecule 1 (STIM1)- and Orai1-deficient cells are resistant to oxidant stress. Functionally, oxidant-induced Ca(2+) entry alters mitochondrial Ca(2+) handling and bioenergetics and triggers cell death. STIM1 is S-glutathionylated at cysteine 56 in response to …

Medical School Watercooler Newsletter - August 8, 2010, Lindsey Lyle

Watercooler Newsletter

This is the August 8, 2010 edition of the Frederick P. Whiddon College of Medicine's newsletter - Watercooler.

Contents Include:

• COM Class of 2014 Introduced To Medical School During Orientation This Week
• Your Top Picks For Online Resources
• Dr. Kathy B. Porter Enters New Chapter
• USA Medical Students Learn Clinical Skills With Standardized Patients
• Eran and N.Q. Adams Endowed Lecture and Visiting Professorship in Neurology to Feature Dr. Phillip A. Low

Growth, Immune And Viral Responses In Hiv Infected African Children Receiving Highly Active Antiretroviral Therapy: A Prospective Cohort Study., Philippa M. Musoke, Peter Mudiope, Linda N. Barlow-Mosha, Patrick Ajuna, Danstan Bagenda, Michael M. Mubiru, Thorkild Tylleskar, Mary G. Fowler

Journal Articles: Epidemiology

BACKGROUND: Scale up of paediatric antiretroviral therapy in resource limited settings continues despite limited access to routine laboratory monitoring. We documented the weight and height responses in HIV infected Ugandan children on highly active antiretroviral therapy and determined clinical factors associated with successful treatment outcomes.

METHODS: A prospective cohort of HIV infected children were initiated on HAART and followed for 48 weeks. Body mass index for age z scores(BAZ), weight and height-for-age z scores (WAZ & HAZ) were calculated: CD4 cell % and HIV-1 RNA were measured at baseline and every 12 weeks. Treatment outcomes were classified according to; both …

Development Of Pyrf-Based Genetic System For Targeted Gene Deletion In Clostridium Thermocellum And Creation Of A Pta Mutant, Shital A. Tripathi, Daniel G. Olson, D. Aaron Argyros, Bethany B. Miller, Trisha F. Barrett, Daniel M. Murphy, Jesse D. Mccool, Anne K. Warner, Vineet B. Rajgarhia, Lee R. Lynd, David A. Hogsett, Nicky C. Caiazza

Dartmouth Scholarship

We report development of a genetic system for making targeted gene knockouts in Clostridium thermocellum, a thermophilic anaerobic bacterium that rapidly solubilizes cellulose. A toxic uracil analog, 5-fluoroorotic acid (5-FOA), was used to select for deletion of the pyrF gene. The ΔpyrF strain is a uracil auxotroph that could be restored to a prototroph via ectopic expression of pyrF from a plasmid, providing a positive genetic selection. Furthermore, 5-FOA was used to select against plasmid-expressed pyrF, creating a negative selection for plasmid loss. This technology was used to delete a gene involved in organic acid production, namely pta, which encodes …

Adherence To The American College Of Surgeons (Acs) Recommendation On Double Gloving, Hand Free Zone And Blunt Suture Needle Use Among Surgeon Ranks, Akbar Nassiry

Theses and Dissertations

Background: The American College of Surgeons guidelines suggest the use of intact gloves, double gloving, hands-free zone technique to pass sharp instruments, and blunt tip suture needles to protect patients, as well as the surgical team. This study estimates the extent to which these guidelines are followed in a large academic health system. Methods: Over a two-month period in the spring of 2010, 320 general surgical attendings, subspecialty surgical attendings, and surgical resident physicians practicing at a large academic health system, were approached during or after surgical conferences to participate in a cross-sectional study. Nearly 1/3rd completed an anonymous and …

Traveling Across America, Togetherness And Memories, Kenneth P. Serbin

At Risk for Huntington's Disease

No abstract provided.

Harnessing The Effect Of Adoptively Transferred Tumor-Reactive T Cells On Endogenous (Host-Derived) Antitumor Immunity, Yolanda Nesbeth, Jose R. Conejo-Garcia

Dartmouth Scholarship

Adoptive T cell transfer therapy, the ex vivo activation, expansion, and subsequent administration of tumor-reactive T cells, is already the most effective therapy against certain types of cancer. However, recent evidence in animal models and clinical trials suggests that host conditioning interventions tailored for some of the most aggressive and frequent epithelial cancers will be needed to maximize the benefit of this approach. Similarly, the subsets, stage of differentiation, and ex vivo expansion procedure of tumor-reactive T cells to be adoptively transferred influence their in vivo effectiveness and may need to be adapted for different types of cancer and host …

Cannabinoid Receptors In The 3d Reconstructed Mouse Brain: Function And Regulation, Peter Nguyen

Theses and Dissertations

CB1 receptors (CB1R) mediate the psychoactive and therapeutic effects of cannabinoids including ∆9-tetrahydrocannabinol (THC), the main psychoactive constituent in marijuana. However, therapeutic use is limited by side effects and tolerance and dependence with chronic administration. Tolerance to cannabinoid-mediated effects is associated with CB1R adaptations, including desensitization (receptor-G-protein uncoupling) and downregulation (receptor degradation). The objectives of this thesis are to investigate the regional-specificity in CB1R function and regulation. Previous studies have investigated CB1Rs in a subset of regions involved in cannabinoid effects, but an inclusive regional comparison of the relative efficacies of different classes of cannabinoids to activate G-proteins has not …