Medicine and Health Sciences Commons^™

Phosphorylation Meets Nuclear Import: A Review., Jonathan D Nardozzi, Kaylen Lott, Gino Cingolani

Department of Biochemistry and Molecular Biology Faculty Papers

Phosphorylation is the most common and pleiotropic modification in biology, which plays a vital role in regulating and finely tuning a multitude of biological pathways. Transport across the nuclear envelope is also an essential cellular function and is intimately linked to many degeneration processes that lead to disease. It is therefore not surprising that phosphorylation of cargos trafficking between the cytoplasm and nucleus is emerging as an important step to regulate nuclear availability, which directly affects gene expression, cell growth and proliferation. However, the literature on phosphorylation of nucleocytoplasmic trafficking cargos is often confusing. Phosphorylation, and its mirror process dephosphorylation, …

Finding High Quality Hospitals In Philadelphia., Robert D. Lieberthal, Phd

College of Population Health Lectures, Presentations, Workshops

Guest lecture for PBH609 GIS Mapping. Fall, 2010 session. Philadelphia, PA.

15 PowerPoint slides.

Feasibility Of Improving Cone Beam Ct Number Consistency With A Scatter-Correction Algorithm, J. Li, W. Yao, Y. Xiao, Y. Yu

Bodine Journal

Purpose: To explore the feasibility of improving Elekta cone beam CT (CBCT) number consistency using a scatter-correction algorithm, with the aim of using CBCT images for treatment planning with heterogeneity correction.

American Association of Physicists in Medicine (AAPM) 52nd Annual Meeting July 18-22, Philadelphia, PA

Acute Neurological Toxicity (Nt) And Long-Term Outcomes In High-Grade Glioma Rtog Trials, Y. R. Lawrence, M. Wang, A. P. Dicker, D. W. Andrews, W. J. Curran, J. M. Michalski, L. Souhami, W. A. Yung, M. Mehta

Bodine Journal

Background: Treatment of high-grade glioma consists of fractionated radiation therapy (RT) ± chemotherapy. The incidence/significance of neurological toxicity (NT) in this disease is not known. We evaluated the relationship between acute and chronic NT, and ultimate outcome, as well as risk factors for NT from the RTOG database.

American Society of Clinical Oncology (ASCO) 46th Annual Meeting June 4-8, Chicago, IL.

Potential For Thermal Enhancement By Quercetin Mediated Mechanisms Targeting P53 Antagonists In Human Melanoma Cells, T. Thangasamy, K. Limesand, D. B. Leeper, R. Burd

Bodine Journal

Introduction: Recently Temozolomide (TMZ) has become the more commonly used analog of DTIC-related oral agents. Although the response rates achieved by TMZ alone are less than satisfactory, there is great interest in identifying compounds that could be used in combination therapy. We have previously demonstrated that the bioflavonoid quercetin (Qct) promotes a p53-mediated response in melanoma and sensitizes melanoma to DTIC. Here we demonstrate that Qct also sensitizes cells to TMZ by a mechanism that involves the modulation of a truncated p53 family member, ΔNp73.

Society for Thermal Medicine Annual Meeting April 23-26, Clearwater Beach, FL.

Control Of Glycolytic Flux By Ampk And P53-Mediated Signaling Pathways In Tumor Cells Adapted To Grow At Low Ph, E. E. Mendoza, J. Caro, D. B. Leeper, R. Burd

Bodine Journal

Introduction: Tumor cells grow in nutrient and oxygen deprived microenvironments and adapt to the suboptimal growth conditions by altering metabolic pathways. This adaptation process characteristically results in a tumor phenotype that displays anaerobic glycolysis, chronic acidification and aggressive tumor characteristics. Understanding the tumor cell reaction to the microenvironment is a critical factor in predicting the tumor response to hyperthermia. The glucose regulatory molecule, 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase Isoform-3 (PFKFB3), is a bifunctional enzyme central to glycolytic flux and downstream of the metabolic stress sensor AMP-activated protein kinase (AMPK), which has been shown to activate an isoform of Phosphofructokinase (PFK-2).

Society for Thermal Medicine …

Functional Antagonism Between Vorinostat And Temozolomide When Combined With Ionizing Radiation (Ir) In Glioblastoma, Y. R. Lawrence, Y. Liu, B. Andersen, X. Xia, A. P. Dicker, P. Wachsberger

Bodine Journal

Background: Glioblastoma is the most common primary adult brain tumor. Surgery followed by radiation therapy in combination with temozolomide (Tmz) produces a median survival of 14.6 months. Tmz is a DNA akylating agent that leads to the mispairing of guanine residues with thymine. An intact mismatch-repair mechanism (MMR) converts the mispaired thymine into a lethal double-strand DNA break. Vorinostat (SAHA), an HDAC inhibitor, has been shown to act as a radiosensitizer, possibly through inhibition of DNA repair. SAHA has successfully been combined with a number of cytotoxic agents. We hypothesized that SAHA would further potentiate the radiosensitizing properties of Tmz …

Effects Of Egfr Expression On Anti-Tumor Efficacy Of Vandetanib Or Cediranib Combined With Radiotherapy (Rt) In U87 Human Glioblastoma (Gbm) Xenografts, P. Wachsberger, Y. R. Lawrence, Y. Liu, A. P. Dicker

Bodine Journal

Introduction: Vandetanib is a receptor tyrosine kinase inhibitor (RTKI) with activity against vascular endothelial growth factor receptor-2 (VEGFR-2) and epidermal growth factor receptor (EGFR). Cediranib is a highly potent VEGF RTKI that inhibits all three VEGF receptors. In this study we investigated the effect of exogenous overexpression of EGFR on sensitivity of human GBM U87 xenografts to vandetanib or cediranib, alone or in combination with RT.

American Association for Cancer Research (AACR) 101st Annual Meeting April 17-21, Washington, DC.

Bodine Journal, Table Of Contents, Volume 3, Issue 1, Fall 2010

Bodine Journal

Table of Contents for Fall 2010 issue of Bodine Journal.

Is The Quality Of Neurosurgical Literature Improving?, James Harrop, Md, Mitchell Maltenfort, Phd

JHN Journal

Introduction:

The advent of evidence-based medicine has resulted in higher quality journal manuscripts in numerous medical disciplines. However, the impact in the neurosurgical literature has not been reported.

Objective:

To quantify the impact of evidence-based medicine on the quality of articles published in the Neurosurgery literature.

Methods:

Articles published in the journal Neurosurgery (founded in 1977) were reviewed for 1978, 1988, 1998, and 2008. Each decade’s sample was classified as therapeutic, diagnostic and prognostic based on a published system for determining level of evidence.

Results:

438 articles were reviewed. Articles not considered included any published under the heading “Case Report” …

Acetate Causes Alcohol Hangover Headache In Rats., Christina R Maxwell, Rebecca Jay Spangenberg, Jan B Hoek, Stephen D Silberstein, Michael L Oshinsky

Department of Neurology Faculty Papers

BACKGROUND: The mechanism of veisalgia cephalgia or hangover headache is unknown. Despite a lack of mechanistic studies, there are a number of theories positing congeners, dehydration, or the ethanol metabolite acetaldehyde as causes of hangover headache.

METHODS: We used a chronic headache model to examine how pure ethanol produces increased sensitivity for nociceptive behaviors in normally hydrated rats.

RESULTS: Ethanol initially decreased sensitivity to mechanical stimuli on the face (analgesia), followed 4 to 6 hours later by inflammatory pain. Inhibiting alcohol dehydrogenase extended the analgesia whereas inhibiting aldehyde dehydrogenase decreased analgesia. Neither treatment had nociceptive effects. Direct administration of acetate …

Biological Rationale For The Use Of Dna Methyltransferase Inhibitors As New Strategy For Modulation Of Tumor Response To Chemotherapy And Radiation., Giovanni L Gravina, Claudio Festuccia, Francesco Marampon, Vladimir M Popov, Richard G Pestell, Bianca M Zani, Vincenzo Tombolini

Kimmel Cancer Center Faculty Papers

Epigenetic modifications play a key role in the patho-physiology of many tumors and the current use of agents targeting epigenetic changes has become a topic of intense interest in cancer research. DNA methyltransferase (DNMT) inhibitors represent a promising class of epigenetic modulators. Research performed yielded promising anti-tumorigenic activity for these agents in vitro and in vivo against a variety of hematologic and solid tumors. These epigenetic modulators cause cell cycle and growth arrest, differentiation and apoptosis. Rationale for combining these agents with cytotoxic therapy or radiation is straightforward since the use of DNMT inhibitor offers greatly improved access for cytotoxic …

Subglottic Secretion Aspiration In The Prevention Of Ventilator-Associated Pneumonia: A Review Of The Literature., Rachel Scherzer

Jefferson Hospital Staff Papers and Presentations

Ventilator-associated pneumonia is a common nosocomial infection that results in both negative patient outcomes and increased health care costs. Recently, many efforts have been targeted at ventilator-associated pneumonia prevention, including the practice of subglottic secretion aspiration. Six randomized control studies examining the effectiveness of subglottic secretion aspiration in the prevention of ventilator-associated pneumonia were reviewed for this article. Results consistently show that subglottic secretion aspiration significantly reduces the incidence of ventilator-associated pneumonia in a variety of patient populations. Despite these findings, this practice is limited in clinical settings. This clinical practice should be implemented in individuals requiring mechanical ventilation to …

Pp32 (Anp32a) Expression Inhibits Pancreatic Cancer Cell Growth And Induces Gemcitabine Resistance By Disrupting Hur Binding To Mrnas., Timothy K Williams, Christina L Costantino, Nikolai A Bildzukewicz, Nathan G Richards, David W Rittenhouse, Lisa Einstein, Joseph A Cozzitorto, Judith C Keen, Abhijit Dasgupta, Myriam Gorospe, Gregory E Gonye, Charles J Yeo, Agnieszka K Witkiewicz, Jonathan R Brody

Department of Surgery Faculty Papers

The expression of protein phosphatase 32 (PP32, ANP32A) is low in poorly differentiated pancreatic cancers and is linked to the levels of HuR (ELAV1), a predictive marker for gemcitabine response. In pancreatic cancer cells, exogenous overexpression of pp32 inhibited cell growth, supporting its long-recognized role as a tumor suppressor in pancreatic cancer. In chemotherapeutic sensitivity screening assays, cells overexpressing pp32 were selectively resistant to the nucleoside analogs gemcitabine and cytarabine (ARA-C), but were sensitized to 5-fluorouracil; conversely, silencing pp32 in pancreatic cancer cells enhanced gemcitabine sensitivity. The cytoplasmic levels of pp32 increased after cancer cells are treated with certain stressors, …

Ms4a4b, A Cd20 Homologue In T Cells, Inhibits T Cell Propagation By Modulation Of Cell Cycle., Hui Xu, Yaping Yan, Mark S Williams, Gregory B Carey, Jingxian Yang, Hongmei Li, Guang-Xian Zhang, Abdolmohamad Rostami

Department of Neurology Faculty Papers

MS4a4B, a CD20 homologue in T cells, is a novel member of the MS4A gene family in mice. The MS4A family includes CD20, FcεRIβ, HTm4 and at least 26 novel members that are characterized by their structural features: with four membrane-spanning domains, two extracellular domains and two cytoplasmic regions. CD20, FcεRIβ and HTm4 have been found to function in B cells, mast cells and hematopoietic cells respectively. However, little is known about the function of MS4a4B in T cell regulation. We demonstrate here that MS4a4B negatively regulates mouse T cell proliferation. MS4a4B is highly expressed in primary T cells, natural …

The Patient Centered Medical Home: Federal, State And Local Initiatives To Transform Primary Care. The First Of An Occasional Series In Interprofessional Education And Care In The Patient-Centered Medical Home, Brooke Salzman

Collaborative Healthcare: Interprofessional Practice, Education and Evaluation (JCIPE)

The Patient Centered Medical Home (PCMH) is rapidly emerging as one prototype for redesigning health care delivery, restructuring reimbursement, and reestablishing the critical value of primary care. The actual term “medical home” was introduced by the American Academy of Pediatrics, (AAP) in 1967, initially referring to a central location for archiving a child’s medical record. In 2002, the AAP expanded the medical home concept to include care characterized as accessible, continuous, comprehensive, patient-centered, coordinated, compassionate, and culturally effective. In 2004, the American Academy of Family Physicians (AAFP) embraced the model in its Future of Family Medicine project report, and in …

Jefferson Award For Excellence In Interprofessional Education

Collaborative Healthcare: Interprofessional Practice, Education and Evaluation (JCIPE)

In recognition for outstanding contributions to interprofessional education two faculty awards were given by JCIPE to Christine Jerpbak, MD, Department of Family and Community Medicine on May 23, 2010 and E. Adel Herge, OTD, OTR/L , Department of Occupational Therapy on June 1, 2010.

Clinical Care Plan, Interprofessional Course, Marcia Levinson, Kathryn Shaffer, Amy Egras

Collaborative Healthcare: Interprofessional Practice, Education and Evaluation (JCIPE)

Evidence suggests interprofessional collaborative practice significantly improves patient outcomes, reduces mortality and enhances quality-of-life.1 Person-focused care demands collaboration among professions in a team approach to address multiple issues including illness, prevention, and health promotion activities. Key elements of successful implementation of interprofessional education are supported in Thomas Jefferson University’s Clinical Care Plan, Interprofessional Course (CCPIC) that includes increasing knowledge of the roles, responsibilities, and competencies of other health professions, collaborating in teams, recognizing the patient as the expert, and communicating effectively.

Developing Interprofessional Teams Using High Fidelity Resuscitation, Ksenia Zukowsky, Kevin Dysart, Brian Glynn, Tara Berman, Judi Ondik, Patricia Constanty, Debbie Cruz

Collaborative Healthcare: Interprofessional Practice, Education and Evaluation (JCIPE)

Interprofessional simulations using scenarios, megacodes, algorithms, and high fidelity equipment provide a closer approximation to what clinicians are likely to face as a team in the actual resuscitation of a newborn infant.

From The Editors, Christine Arenson, Md, Molly Rose, Rn, Phd

Collaborative Healthcare: Interprofessional Practice, Education and Evaluation (JCIPE)

Welcome to the latest edition of the Jefferson Interprofessional Education and Care Newsletter. In this issue, Dr. Zukowsky and colleagues describe the value of high-fidelity simulation to train interprofessional teams in high-risk high-stakes care. Their successful model has important implications for preparing teams to perform optimally during critical, and often stressful, clinical situations where expertise in both technical and team skills is imperative for best patient outcomes. Dr. Levinson and colleagues describe an exciting interprofessional care planning course which, for the first time at Jefferson, has brought senior Occupational Therapy (OT), Physical Therapy (PT), Nursing, Pharmacy and Medical students together …

Download Entire Pdf Interprofessional Education And Care Newsletter, Vol. 2, No. 1, Fall 2010

Collaborative Healthcare: Interprofessional Practice, Education and Evaluation (JCIPE)

This issue includes articles on:

Developing Interprofessional Teams using High Fidelity Resuscitation

Clinical Care Plan, Interprofessional Course

The Patient Centered Medical Home: Federal, State and Local Initiatives to Transform Primary Care The First of an Occasional Series in Interprofessional Education and Care in the Patient-Centered Medical Home

JCIPE hosted its First International Interprofessional Education and Care Conference

A New Frontier For Headache., Stephen Silberstein

Department of Jefferson Headache Center papers and presentations

No abstract provided.

Those Who Give: The Kristen Olewine Milke Breast Cancer Research Fund

Jefferson Surgical Solutions

No abstract provided.

Dr. Adam Berger Heads New Section Of Surgical Oncology

Jefferson Surgical Solutions

No abstract provided.

Meet Our Surgical Interns

Jefferson Surgical Solutions

No abstract provided.

Vascular Center Offers Leading- Edge, Integrated Care

Jefferson Surgical Solutions

No abstract provided.

Overview: Charles J. Yeo, Md

Jefferson Surgical Solutions

No abstract provided.

About The Surgeon

Jefferson Surgical Solutions

No abstract provided.

What Makes A Surgeon Of Choice: Dr. John Maxwell

Jefferson Surgical Solutions

No abstract provided.

Differential Regulation Of P53 Function By The N-Terminal Δnp53 And Δ113p53 Isoforms In Zebrafish Embryos., William R Davidson, Csaba Kari, Qing Ren, Borbala Daroczi, Adam P Dicker, Ulrich Rodeck

Department of Radiation Oncology Faculty Papers

BACKGROUND: The p53 protein family coordinates stress responses of cells and organisms. Alternative promoter usage and/or splicing of p53 mRNA gives rise to at least nine mammalian p53 proteins with distinct N- and C-termini which are differentially expressed in normal and malignant cells. The human N-terminal p53 variants contain either the full-length (FL), or a truncated (ΔN/Δ40) or no transactivation domain (Δ133) altogether. The functional consequences of coexpression of the different p53 isoforms are poorly defined. Here we investigated functional aspects of the zebrafish ΔNp53 ortholog in the context of FLp53 and the zebrafish Δ133p53 ortholog (Δ113p53) coexpressed in the …