Medicine and Health Sciences Commons^™

Novel Molecules For Intra-Oral Delivery Of Antimicrobials To Prevent And Treat Oral Infectious Diseases, Periathamby A. Raj, Latha Rajkumar, Andrew R. Dentino

School of Dentistry Faculty Research and Publications

New molecules were designed for efficient intra-oral delivery of antimicrobials to prevent and treat oral infection. The salivary statherin fragment, which has high affinity for the tooth enamel, was used as a carrier peptide. This was linked through the side chain of the N-terminal residue to the C-terminus of a defensin-like 12-residue peptide to generate two bifunctional hybrid molecules, one with an ester linkage and the other with an anhydride bond between the carrier and the antimicrobial components. They were examined for their affinity to a HAP (hydroxyapatite) surface. The extent of the antimicrobial release in human whole saliva was …

Managing Severe Pneumonia In Children In Developing Countries - Increasing Resistance To First Line Antibiotics Means Recommendations Need Changing, Zulfiqar Ahmed Bhutta

Department of Paediatrics and Child Health

No abstract provided.

Estimation Of Controlled Direct Effects, Sylvie Goetgeluk, Stijn Vansteelandt, Els Goetghebeur

Harvard University Biostatistics Working Paper Series

No abstract provided.

A Comparison Of Perinatal Care Providers' Use Of The National Institute Of Child Health And Human Development Standardized Terminology In Documentation Of Intrapartal Fetal Heart Rate Patterns, Faye M. Sigman

Dissertations

The purpose of this study was to determine if perinatal team members; nurses (RN) and primary care providers (PCP), were using the NICHD standardized terminology to document Fetal Heart Rate patterns during labor. Agreement in documentation of FHR and agreement in concept between the RN and PCP was also studied. A descriptive, comparative research design was used. Cohen?s Kappa statistics measured agreement in documentation of FHR patterns and Chi square measured agreement in concept, p< 0.05 for each. A retrospective medical records chart review was performed on 400 charts, meeting inclusion criteria, from three community hospitals. There were three data collection points and four criteria reviewed. This study found the use of NICHD terminology to document FHR alarmingly low (RN=51%; PCP=13%). It was used most often for decelerations (81%) RN, (22%) PCP, and least often for variability (19%) RN, (3%) PCP. Incomplete documentation was extremely high for the PCP (69%) and 81 charts (20%) had no FHR documentation. Agreement in documentation varied between the RN and PCP. They agreed most often on accelerations (81.4%) and least often on baseline rate (41.5%). When looking at all there points in time the RN and PCP agreed in documentation 59% but agreed in concept 78%. There were four areas where the RN and PCP agreed in their use of NICHD terminology: Accelerations on admission n=151, Kappa=0.091, p=0.007; variability during labor n=68, Kappa=0.27, p=0.015; variability prior to delivery n=33 Kappa=0.33, p=0.010, and decelerations during labor n=103, Kappa=0.16, p=0.018. Data from this study supports expanding this research, to identifying barriers to documentation. It also appears that education in use of NICHD terminology is needed.

Attention And Emotion Influence The Relationship Between Extraversion And Neural Response, C. A. Hutcherson, P. R. Goldin, W. Ramel, Kateri Mcrae, J. J. Gross

Psychology: Faculty Scholarship

Extraversion has been shown to positively correlate with activation within the ventral striatum, amygdala and other dopaminergically innervated, reward-sensitive regions. These regions are implicated in emotional responding, in a manner sensitive to attentional focus. However, no study has investigated the interaction among extraversion, emotion and attention. We used fMRI and dynamic, evocative film clips to elicit amusement and sadness in a sample of 28 women. Participants were instructed either to respond naturally (n = 14) or to attend to and continuously rate their emotions (n = 14) while watching the films. Contrary to expectations, striatal response was negatively associated with …

Amp-Activated Protein Kinase Regulates Co2-Induced Alveolar Epithelial Dysfunction In Rats And Human Cells By Promoting Na,K-Atpase Endocytosis, István Vadász, Laura A. Dada, Arturo Briva, Humberto E. Trejo, Lynn C. Welch, Jiwang Chen, Peter T. Toth, Emilia Lecuona, Lee A. Witters, Paul T. Schumacker, Navdeep S. Chandel, Werner Seeger, Jacob I. Sznajder

Dartmouth Scholarship

Hypercapnia (elevated CO₂ levels) occurs as a consequence of poor alveolar ventilation and impairs alveolar fluid reabsorption (AFR) by promoting Na,K-ATPase endocytosis. We studied the mechanisms regulating CO₂-induced Na,K-ATPase endocytosis in alveolar epithelial cells (AECs) and alveolar epithelial dysfunction in rats. Elevated CO₂ levels caused a rapid activation of AMP-activated protein kinase (AMPK) in AECs, a key regulator of metabolic homeostasis. Activation of AMPK was mediated by a CO₂-triggered increase in intracellular Ca²⁺ concentration and Ca²⁺/calmodulin-dependent kinase kinase-β (CaMKK-β). Chelating intracellular Ca²⁺ or abrogating CaMKK-β function by gene silencing or …

Kimbrough, William Joseph, Jr., 1930-2007 (Sc 1516), Manuscripts & Folklife Archives

Manuscript Collection Finding Aids

Finding aid only for Manuscripts Small Collection 1516. Correspondence between Kimbrough and Sue Lynn (Stone) McDaniel, a WKU faculty member in the Kentucky Building. Letters discuss Kimbrough's collection of letters, his years as a student at Western (1948-1952), and his bout with cancer.

Gene Expression Profile Of Neuronal Progenitor Cells Derived From Hescs: Activation Of Chromosome 11p15.5 And Comparison To Human Dopaminergic Neurons., William J Freed, Jia Chen, Cristina M Bäckman, Catherine M Schwartz, Tandis Vazin, Jingli Cai, Charles E Spivak, Carl R Lupica, Mahendra S Rao, Xianmin Zeng

Farber Institute for Neuroscience Faculty Papers

BACKGROUND: We initiated differentiation of human embryonic stem cells (hESCs) into dopamine neurons, obtained a purified population of neuronal precursor cells by cell sorting, and determined patterns of gene transcription.

METHODOLOGY: Dopaminergic differentiation of hESCs was initiated by culturing hESCs with a feeder layer of PA6 cells. Differentiating cells were then sorted to obtain a pure population of PSA-NCAM-expressing neuronal precursors, which were then analyzed for gene expression using Massive Parallel Signature Sequencing (MPSS). Individual genes as well as regions of the genome which were activated were determined.

PRINCIPAL FINDINGS: A number of genes known to be involved in the …

Manual For The Motivational Interviewing Skill Code (Misc), Paul Amrhein, William R. Miller, Theresa Moyers, Denise Ernst

Department of Psychology Faculty Scholarship and Creative Works

Over years of using MISC 1 we have learned much about which categories were redundant or unreliable, and also about which processes are most important to the effectiveness of MI. The MISC has also helped us to clarify the points at which skill acquisition in MI is more challenging.

Based on this experience, we have developed Version 2.0, which is intended to improve on the original MISC in reliability, efficiency, and relevance to training and clinical practice. A disadvantage of revising an instrument, of course, is that one must start over in demonstrating its reliability and validity. Although many strong …

Ada News - 01/07/2008, American Dental Association, Publishing Division

ADA News

Established in 1970 as the official newspaper of the American Dental Association, the ADA News serves practicing dentists and others allied to the dental profession in the U.S. and internationally. It is the No. 1 source of news and information about the many benefits and services the ADA delivers to members daily as well as timely information on scientific, social, political and economic developments affecting dentistry and health care.

Alternate Cyclin D1 Mrna Splicing Modulates P27Klp1 Binding And Cell Migration, Zhiping Li, Chenguang Wang, Xuanmao Jiao, Sanjay Katiyar, Mathew C. Casimiro, George C. Prendergast, Michael J. Powell, Richard G. Pestell

Department of Cancer Biology Faculty Papers

Cyclin D1 is an important cell cycle regulator but in cancer its overexpression also increases cellular migration mediated by p27^KlP1 stabilization and RhoA inhibition. Recently, a common polymorphism at the exon 4-intron 4 boundary of the human cyclin D1 gene within a splice donor region was associated with an altered risk of developing cancer. Altered RNA splicing caused by this polymorphism gives rise to a variant cyclin D1 isoform termed cyclin D1b, which has the same N-terminus as the canonical cyclin D1a isoform but a distinct C-terminus. In this study we show that these different isoforms have unique properties …

Knowledge And Utilization Of The Perioperative Nursing Data Set By Perioperative Staff Nurses In Southeastern Michigan, Jill Ortmann

Master's Theses and Doctoral Dissertations

The purpose of this descriptive/correlational study was to describe what perioperative nurses practicing in southeastern Michigan know about the Perioperative Nursing Data Set (PNDS), how they are utilizing the PNDS, and their opinion of the PNDS, and also to examine the relationship between these variables and demographic data. Four research questions were addressed by the use of a questionnaire.

The results of this study were drawn from the return of 151 questionnaires out of a possible 319 questionnaires submitted to practicing perioperative registered nurses (RNs) in southeastern Michigan (47 percent return rate). These results showed that overall, perioperative RNs did …

Early Life Determinants Of Physical Activity In 11 To 12 Year Olds: Cohort Study, Calum Mattocks, Kevin Deere, Sam D. Leary, Andy R. Ness, Kate Tilling, Steven N. Blair, Chris J. Riddoch

Faculty Publications

Objective: To examine factors in early life (up to age 5 years) that are associated with objectively measured physical activity in 11-12 year olds.

Design: Prospective cohort study

Setting: Avon longitudinal study of parents and children, United Kingdom.

Participants: Children aged 11-12 years from the Avon longitudinal study of parents and children.

Main outcome measure: Physical activity levels in counts per minute (cpm) and minutes of moderate to vigorous physical activity for seven days measured with a uniaxial actigraph accelerometer.

Results: Valid actigraph data, defined as at least three days of physical activity for at least 10 hours a day, …

Differential Role Of Nadp+ And Nadph In The Activity And Structure Of Gdp-D-Mannose 4,6-Dehydratase From Two Chlorella Viruses, Floriana Fruscione, Laura Sturla, Garry Duncan, James L. Van Etten, Paola Valbuzzi, Antonio De Flora, Eleonopra Di Zanni, Michela Tonetti

James Van Etten Publications

GDP-D-mannose 4,6-dehydratase (GMD) is a key enzyme involved in the synthesis of 6-deoxyhexoses in prokaryotes and eukaryotes. Paramecium bursaria chlorella virus-1 (PBCV-1) encodes a functional GMD, which is unique among characterized GMDs because it also has a strong stereospecific NADPH-dependent reductase activity leading to GDP-D-rhamnose formation (Tonetti, M., Zanardi, D., Gurnon, J., Fruscione, F., Armirotti, A., Damonte, G., Sturla, L., De Flora, A., and Van Etten, J.L. (2003) J. Biol. Chem. 278, 21559–21565). In the present study we characterized a recombinant GMD encoded by another chlorella virus, Acanthocystis turfacea chlorella virus 1 (ATCV-1), demonstrating that it has the expected dehydratase …

Repression Of Hla By Rot Is Dependent On Sae In Staphylococcus Aureus, Dongmei Li, Ambrose Cheung

Dartmouth Scholarship

The regulatory locus sae is a two-component system in Staphylococcus aureus that regulates many important virulence factors, including alpha-toxin (encoded by hla) at the transcriptional level. The SarA homologs Rot and SarT were previously shown to be repressors of hla in selected S. aureus backgrounds. To delineate the interaction of rot and sae and the contribution of sarT to hla expression, an assortment of rot and sae isogenic single mutants, a rot sae double mutant, and a rot sae sarT markerless triple mutant were constructed from wild-type strain COL. Using Northern blot analysis and transcriptional reporter gene green fluorescent protein, …

Nitrated Alpha-Synuclein Immunity Accelerates Degeneration Of Nigral Dopaminergic Neurons., Eric J. Benner, Rebecca Banerjee, Ashley D. Reynolds, Simon Sherman, Vladimir M. Pisarev, Vladislav Tsiperson, Craig Nemachek, Pawel Ciborowski, Serge Przedborski, R. Lee Mosley, Howard Gendelman

Journal Articles: Eppley Institute

BACKGROUND: The neuropathology of Parkinson's disease (PD) includes loss of dopaminergic neurons in the substantia nigra, nitrated alpha-synuclein (N-alpha-Syn) enriched intraneuronal inclusions or Lewy bodies and neuroinflammation. While the contribution of innate microglial inflammatory activities to disease are known, evidence for how adaptive immune mechanisms may affect the course of PD remains obscure. We reasoned that PD-associated oxidative protein modifications create novel antigenic epitopes capable of peripheral adaptive T cell responses that could affect nigrostriatal degeneration.

METHODS AND FINDINGS: Nitrotyrosine (NT)-modified alpha-Syn was detected readily in cervical lymph nodes (CLN) from 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) intoxicated mice. Antigen-presenting cells within the CLN …

Developing An International Competence-Based Curriculum For Environmental Health, R. Steven Konkel, Maurice Brennan, Tony Lewis

Environmental Health Science Faculty and Staff Research

In 1998, the International Federation of Environmental Health (IEFH) commissioned the International Faculty Forum (IFF) of environmental health educators to develop an international curriculum for environmental health. In commissioning such a curriculum, IFEH implicitly recognised and sought to address the ongoing issues of professional identity, status and the transportability of qualifications for Environmental Health Practitioners (EHPs). A draft model for an international curriculum based on competence was proposed by Brennan, Konkel and Lewis and developed and supported by IFF members when they met in May 2008 in Brisbane, Australia. Development of the model and its underpinning concepts of 'environmental healthiness' …

Role Of Transient Receptor Potential Canonical-6 (Trpc6) Channel In Metastasis Of Glioblastoma Multiforme, Rajarajeshwari Venkataraman

Electronic Theses and Dissertations

Glioblastoma multiforme (GBM) is one of the extremely fatal brain tumors. The main reason that makes it so lethal is its capability to invade and spread to other parts of CNS producing secondary tumors. Among other factors hypoxia, reduced oxygen availability, is linked to higher metastatic potential of cancers. Hypoxia causes numerous changes in genome and proteome of the cell. These changes help a normal cell to adapt to nutritional deficiency, but the same changes can increase the malignancy and metastasis in tumor cells. Extensive research by a number of curious scientists reveal that various pathways involving numerous proteins cross-talk …

Evaluation Of The Efficacy Of Chloroplast-Derived Antigensagainst Malaria, Melissa Schreiber

Electronic Theses and Dissertations

Malaria is the most prevalent vector-borne parasitic disease worldwide and a major cause of death from infections. There is a great need to develop a low cost vaccine for malaria to control transmission of infection and impact of disease, due to the emergence of anti-malarial resistance. Two leading blood stage malarial vaccine candidates are the apical membrane antigen-1 (AMA-1) and the merozoite surface protein-1 (MSP-1). The aim of this project is to express malarial antigens in tobacco plants via plastid transformation and deliver them by subcutaneous or oral gavage of minimally processed transplastomic tissue to evaluate their efficacy to elicit …

Caduceus 2008, Stritch School Of Medicine

Stritch School of Medicine Yearbooks

No abstract provided.

The Clinical Efficacy Of Reflexology In Nursing Home Residents With Dementia, Nancy A. Hodgson

Selected Works of Nancy Hodgson, PhD

No abstract provided.

Evaluation Of Work Place Group And Internet Based Physical Activity Interventions On Psychological Variables Associated With Exercise Behavior Change, Kimberley A. Dawson, Jill Tracey, Tanya Berry

Kinesiology and Physical Education Faculty Publications

The purpose of this research was to compare group-based and internet-based physical activity interventions in terms of desirability, participants characteristics, exercise self-efficacy, and barrier self-efficacy. Pretest questionnaires were completed prior to voluntary enrollment into either of the ten-week physical activity interventions. Both interventions were based on Social Cognitive Theory and the Transtheoretical Model. Interventions were followed with posttest questionnaires. Results demonstrated that the internet intervention attracted more participants, but only the grou-based participants showed significant increases in exercise and barrier self-efficacy. At pretest, participants who selected the internet intervention were significantly lower in life and job satisfaction than those who …

Built Environment And Physical Functioning In Hispanic Elders: The Role Of “Eyes On The Street”, Scott C. Brown, Craig A. Mason, Tatiana Perrino, Joanna L. Lombard, Frank Martinez, Elizabeth Plater-Zyberk, Arnold R. Spokane, José Szapocznik

School of Architecture Articles and Papers

Background: Research on neighborhood effects increasingly includes the influences of the built environment on health and social well-being.

Objectives: In this population-based study in a low-socioeconomic-status (SES), Hispanic neighborhood, we examined whether architectural features of the built environment theorized to promote direct observations and interactions (e.g., porches, stoops) predicted Hispanic elders’ social support and psychological and physical functioning.

Methods: We coded built-environment features for all 3,857 lots in the 403-block area of an urban Miami, Florida, community. We then conducted three annual assessments of social support, psychological distress, and physical functioning in a population-based sample of 273 low-SES Hispanic elders …

Economic Evaluation Of Continuing Care Interventions In The Treatment Of Substance Abuse, Michael T. French, Ioana Popovici, James R. Mckay

Sociology Faculty Articles and Papers

The chronic and relapsing nature of substance abuse points to the need for continuing care after a primary phase of treatment. This article reviews the economic studies of continuing care, discusses research gaps, highlights some of the challenges of conducting rigorous economic evaluations of continuing care, and offers research guidelines and recommendations for future economic studies in this emerging field. Rigorous economic evaluations are needed by health care providers and policy makers to justify the allocation of scarce resources to continuing care interventions. The adoption of cost-effective continuing care services can reduce long-term consequences of addiction, thereby potentially increasing overall …

Understanding The Nature And Impact Of Alcoholism : Implications For Ministry In Kenya, Margaret Kemunto Obaga

Master of Theology Theses

No abstract provided.

Graduate Bulletin, 2008-2010, Minnesota State University Moorhead

Graduate Bulletins (Catalogs)

No abstract provided.

An Occupational Therapist's Guide For Rehabilitative Driving With Traumatic Brain Injured Clients, Josiah Hadsall

Occupational Therapy Capstones

Traumatic brain injuries are devastating occurrence accounting for nearly 10 million injuries occurring each year, with 2 million of those occurring in the United States. As these individuals progress through rehabilitation and begin to acquire independence once again, they look for opportunities to reintegrate within the communities which they live. Driving has been identified as a monumental stage of rehabilitation and is a key way to experience the community for individuals after a traumatic brain injury. This scholarly project was conducted to help occupational therapists addressing driving rehabilitation with traumatic brain injured clients and help ease some of the problems …

Healthnet News V.23:No.4 Winter 2008, Judith S. Kronick, Alberta Richetelle

Articles - Patient Care

A newsletter for public librarians and others interested in consumer health information services

Agni: The Vital Source Of Life, Amala Guha

SoM Articles

No abstract provided.

Annual Report Information Technology 2007-2008

Annual Reports - Education

Information Technology Annual Report Fiscal Year 2007-2008. Sandra Armstrong, Chief Information Officer, University of Connecticut Health Center.