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Articles 1 - 30 of 101
Full-Text Articles in Medicine and Health Sciences
Kajian Keamanan Pemakaian Obat Anti-Hipertensi Di Poliklinik Usia Lanjut Instalasi Rawat Jalan Rs Dr Sardjito, Zullies Ikawati
Kajian Keamanan Pemakaian Obat Anti-Hipertensi Di Poliklinik Usia Lanjut Instalasi Rawat Jalan Rs Dr Sardjito, Zullies Ikawati
Majalah Ilmu Kefarmasian
Increasing age is generally followed by increasing chronic diseases so that the elderly needs much therapy using drugs for therapy of numerous diseases they have. Hyper-tension is one of diseases of which its prevalence increases along with increasing age. Most of the elderly diagnosed as having hypertension finally take therapy using anti-hypertension drugs. Physiological changes that happen to the elderly lead to use of drugs for side effect diseases of which their consumption should be considered while having anti-hypertension drugs. Changes in biological system to the elderly will af-fect the process of drug molecular interaction, which finally affects clinical efficacy …
Incentives For Orphan Drug Research And Development In The United States, Enrique Seoane-Vazquez, Rosa Rodriguez-Monguio, Sheryl L. Szeinbach, Jay Visaria
Incentives For Orphan Drug Research And Development In The United States, Enrique Seoane-Vazquez, Rosa Rodriguez-Monguio, Sheryl L. Szeinbach, Jay Visaria
Pharmacy Faculty Articles and Research
Background: The Orphan Drug Act (1983) established several incentives to encourage the development of orphan drugs (ODs) to treat rare diseases and conditions. This study analyzed the characteristics of OD designations, approvals, sponsors, and evaluated the effective patent and market exclusivity life of orphan new molecular entities (NMEs) approved in the US between 1983 and 2007.
Methods: Primary data sources were the FDA Orange Book, the FDA Office of Orphan Drugs Development, and the US Patent and Trademark Office. Data included all orphan designations and approvals listed by the FDA and all NMEs approved by the FDA during …
Synthesis Of Marine Chemicals And Derivatives As Potential Anti-Cancer Drugs., Laude Bannerman-Akwei
Synthesis Of Marine Chemicals And Derivatives As Potential Anti-Cancer Drugs., Laude Bannerman-Akwei
Electronic Theses and Dissertations
Two natural marine compounds, 3-bromo-4,5-dihydroxybenzaldehyde 2 and 2,3-dibromo-4,5-dihydroxybenzaldehyde 5 together with two novel derivatives, 3-bromo-5-(tert-butyl-dimethyl-silanyloxy)-4-hydroxybenzaldehyde 3 and 1-bromo-2,3-dimethoxy-5-nitrooxy-methylbenzene 9, were synthesized. Compounds 2, 3, and 5 were evaluated for their biological activity towards the inhibition of prostate cancer cell growth using staurosporine a a positive control. All three compounds have shown significant inhibition of prostate cancer cell growth. Compound 9 is yet to be evaluated.
Cytotoxic Effects Of Ruthenium Compounds On Human Cancer Cell Lines., Katie Beth Brown
Cytotoxic Effects Of Ruthenium Compounds On Human Cancer Cell Lines., Katie Beth Brown
Electronic Theses and Dissertations
Chemotherapy is the most common cancer treatment. Traditionally, platinum-based drugs are used in chemotherapy. More recently, researchers have focused on ruthenium based compounds as a substitute for the platinum compounds. Ruthenium-based compounds appear to be less toxic to healthy cells than traditional platinum-based compounds. In this study, 7 ruthenium-based compounds were tested on HT-29 (colon) and MCF-7 (breast) human cancer cell lines with the specific aim of determining whether or not any of the ruthenium-based compounds exhibited cytotoxic properties. In addition, levels of vascular endothelial growth factor (VEGF) production were tested in supernate from the cancer cells treated with various …
Medications That Should Not Be Crushed, K. Shaver, Joseph A. Woelfel
Medications That Should Not Be Crushed, K. Shaver, Joseph A. Woelfel
School of Pharmacy Faculty Articles
No abstract provided.
Manufactured Aluminum Oxide Nanoparticles Decrease Expression Of Tight Junction Proteins In Brain Vasculature, Lei Chen, Robert A. Yokel, Bernhard Hennig, Michal Toborek
Manufactured Aluminum Oxide Nanoparticles Decrease Expression Of Tight Junction Proteins In Brain Vasculature, Lei Chen, Robert A. Yokel, Bernhard Hennig, Michal Toborek
Pharmaceutical Sciences Faculty Publications
Manufactured nanoparticles of aluminum oxide (nano-alumina) have been widely used in the environment; however, their potential toxicity provides a growing concern for human health. The present study focuses on the hypothesis that nano-alumina can affect the blood-brain barrier and induce endothelial toxicity. In the first series of experiments, human brain microvascular endothelial cells (HBMEC) were exposed to alumina and control nanoparticles in dose- and time-responsive manners. Treatment with nano-alumina markedly reduced HBMEC viability, altered mitochondrial potential, increased cellular oxidation, and decreased tight junction protein expression as compared to control nanoparticles. Alterations of tight junction protein levels were prevented by cellular …
Structure-Activity Relationship And Mechanistic Studies On The Chemopreventive Activity Of Dipyridamole And Its Analogues, Ja’Wanda Shavon Grant
Structure-Activity Relationship And Mechanistic Studies On The Chemopreventive Activity Of Dipyridamole And Its Analogues, Ja’Wanda Shavon Grant
Theses and Dissertations (ETD)
There is an escalating demand in the area of cancer prevention and control for the development of novel agents and molecular targets that have potential to reduce the incidence of cancer. In this study, the JB6 mouse epidermal cell-culture based transformation model was used in efforts to identify novel chemopreventive agents and targets. The nucleoside transport inhibitor dipyridamole (DPM) showed potent chemopreventive activity against JB6 P+cells (tumor promotion sensitive). To probe the effects of DPM structural features on its antitumor promotion activity, the soft-agar colony forming efficiency assay was used to screen an in-house library of …
Structure- And Ligand-Based Design Of Novel Antimicrobial Agents, Kirk Edward Hevener
Structure- And Ligand-Based Design Of Novel Antimicrobial Agents, Kirk Edward Hevener
Theses and Dissertations (ETD)
The use of computer based techniques in the design of novel therapeutic agents is a rapidly emerging field. Although the drug-design techniques utilized by Computational Medicinal Chemists vary greatly, they can roughly be classified into structure-based and ligand-based approaches. Structure-based methods utilize a solved structure of the design target, protein or DNA, usually obtained by X-ray or NMR methods to design or improve compounds with activity against the target. Ligand-based methods use active compounds with known affinity for a target that may yet be unresolved. These methods include Pharmacophore-based searching for novel active compounds or Quantitative Structure-Activity Relationship (QSAR) studies. …
Synthesis And Evaluation Of Tetramic Acids As Antimicrobial Agents, Jason B. Wilson
Synthesis And Evaluation Of Tetramic Acids As Antimicrobial Agents, Jason B. Wilson
Theses and Dissertations (ETD)
As bacterial infectious diseases are a major cause of morbity and mortality throughout the world, and many causative organisms are resistant to currently available antibiotics, the motivation for the development of new drugs is readily apparent. A number of natural products exhibiting antimicrobial activity possess a tetramic acid (2,4-pyrrolidinedione) functional group. As their antibacterial mechanism of action is different from that of many of the currently available antibiotics, these compounds have potential to serve as a basis for a pharmacophore in synthetic compounds. However, toxicity to eukaryotic cells is frequently a problem with currently known tetramic acids. The purpose of …
Controlled Release Of Insulin And Modified Insulin From A Novel Injectable Biodegradable Gel, Om Anand
Controlled Release Of Insulin And Modified Insulin From A Novel Injectable Biodegradable Gel, Om Anand
Theses and Dissertations (ETD)
The objective of the study was to develop a controlled release dosage form of
insulin, which can provide basal concentrations of insulin in diabetic rats for 1 to 2 weeks after a single subcutaneous injection.
A biodegradable injectable drug delivery gel was prepared by dissolving a
biodegradable polymer, polylactic-co-glycolic acid (PLGA), in biocompatible
plasticizer(s), triethyl citrate (TEC) and/or acetyl triethyl citrate (ATEC). Insulin was
then loaded into the blank gel to form an insulin suspension in the polymer solution.
After the insulin-loaded gel was injected subcutaneously, the plasticizer(s) dissolved in the aqueous media and were gradually taken away from the …
Aluminum Bioavailability From Tea Infusion, Robert A. Yokel, Rebecca L. Florence
Aluminum Bioavailability From Tea Infusion, Robert A. Yokel, Rebecca L. Florence
Pharmaceutical Sciences Faculty Publications
The objective was to estimate oral Al bioavailability from tea infusion in the rat, using the tracer 26Al. 26Al citrate was injected into tea leaves. An infusion was prepared from the dried leaves and given intra-gastrically to rats which received concurrent intravenous 27Al infusion. Oral Al bioavailability (F) was calculated from the area under the 26Al, compared to 27Al, serum concentration × time curves. Bioavailability from tea averaged 0.37%; not significantly different from water (F = 0.3%), or basic sodium aluminum phosphate (SALP) in cheese (F = 0.1 to 0.3%), but greater than acidic SALP …
Permissive Underfeeding In Trauma Patients, L. Current, Jane Gervasio, J. Ash, L. Bortenschlager, T. Pohlman
Permissive Underfeeding In Trauma Patients, L. Current, Jane Gervasio, J. Ash, L. Bortenschlager, T. Pohlman
Jane M. Gervasio
Abstract from the ASHP Midyear Clinical Meeting, Orlando, FL, December 6-11, 2008.
Feeding Intolerance Of Patients Placed In Pentobarbital-Induced Coma At Methodist Hospital, J. Egel, J. Mcgehee, D. Stillabower, N. Ponton, Jane Gervasio
Feeding Intolerance Of Patients Placed In Pentobarbital-Induced Coma At Methodist Hospital, J. Egel, J. Mcgehee, D. Stillabower, N. Ponton, Jane Gervasio
Jane M. Gervasio
Abstract from the ASHP Midyear Clinical Meeting, Orlando, FL, December 6-11, 2008.
In Vitro Models For Inhaled Corticosteroid (Ics) Aerosols: A Study Of Their Biopharmaceutics And Pharmacology, Deepika Arora
In Vitro Models For Inhaled Corticosteroid (Ics) Aerosols: A Study Of Their Biopharmaceutics And Pharmacology, Deepika Arora
Theses and Dissertations
Lung cellular disposition and anti-inflammatory pharmacology of inhaled corticosteroids (ICSs) is complex, comprised of a cascade of aerosol deposition and dissolution, followed by cellular uptake for local pharmacological action. This project hypothesized that the kinetics of dissolution for certain ICS aerosols generated from inhaler products were kinetically rate-determined for their cellular uptake and local pharmacological action. A novel dissolution testing system was developed to determine the dissolution kinetics for the ICS aerosols. A total of 5 ICSs aerosols generated from 6 inhaler products were collected in 2.1-3.3 or 4.7-5.8 µm of aerodynamic diameters at 0.7-19.8 µg on filter membranes by …
A Novel Gβγ-Subunit Inhibitor Selectively Modulates Μ-Opioid- Dependent Antinociception And Attenuates Acute Morphine-Induced Antinociceptive Tolerance And Dependence, Jennifer L. Mathews, Alan V. Smrcka, Jean M. Bidlack
A Novel Gβγ-Subunit Inhibitor Selectively Modulates Μ-Opioid- Dependent Antinociception And Attenuates Acute Morphine-Induced Antinociceptive Tolerance And Dependence, Jennifer L. Mathews, Alan V. Smrcka, Jean M. Bidlack
Pharmacy Faculty/Staff Publications
No abstract provided.
Sequiterpene Synthase Gene And Protein, Joe Chappell, Bryan T. Greenhagen
Sequiterpene Synthase Gene And Protein, Joe Chappell, Bryan T. Greenhagen
Pharmaceutical Sciences Faculty Patents
The invention relates to sesquiterpene synthases and methods for their production and use. Particularly, the invention provides nucleic acids comprising the nucleotide sequence of citrus valencene synthase (CVS) which codes for at least one CVS. The invention further provides nucleic acids comprising the nucleotide sequence coding for amino acid residues forming the tier 1 and tier 2 domains of CVS. The invention also provides for methods of making and using the nucleic acids and amino acids of the current invention.
High-Activity Mutants Of Butyrylcholinesterase For Cocaine Hydrolysis And Method Of Generating The Same, Chang-Guo Zhan, Hoon Cho, Hsin-Hsiung Tai
High-Activity Mutants Of Butyrylcholinesterase For Cocaine Hydrolysis And Method Of Generating The Same, Chang-Guo Zhan, Hoon Cho, Hsin-Hsiung Tai
Pharmaceutical Sciences Faculty Patents
A novel computational method and generation of mutant butyrylcholinesterase for cocaine hydrolysis is provided. The method includes molecular modeling a possible BChE mutant and conducting molecular dynamics simulations and hybrid quantum mechanical/molecular mechanical calculations thereby providing a screening method of possible BChE mutants by predicting which mutant will lead to a more stable transition state for a rate determining step. Site-directed mutagenesis, protein expression, and protein activity is conducted for mutants determined computationally as being good candidates for possible BChE mutants, i.e., ones predicted to have higher catalytic efficiency as compared with wild-type BChE. In addition, mutants A199S/A328W/Y332G, A199S/F227A/A328W/Y332G, A199S/S287G/A328W/Y332G, …
Helical Peptides Derived From Lactoferrin Bind Hepatitis C Virus Envelope Protein E2, Reem Beleid, Donna Douglas, Norman Kneteman, Kamaljit Kaur
Helical Peptides Derived From Lactoferrin Bind Hepatitis C Virus Envelope Protein E2, Reem Beleid, Donna Douglas, Norman Kneteman, Kamaljit Kaur
Pharmacy Faculty Articles and Research
Hepatitis C virus is a major cause of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma infecting more than 170 million people. Hepatitis C virus envelope 2 glycoprotein (E2) binds several cell‐surface molecules that act as receptor candidates mediating hepatitis C virus entry into hepatocytes. Peptides derived from human lactoferrin have been shown to bind hepatitis C virus‐E2 protein thereby preventing hepatitis C virus entry in cultured hepatocytes. In this study, starting from a 33‐residue human lactoferrin‐derived peptide, a number of biotin‐linked α‐peptides were synthesized and investigated for their E2 protein binding activity. E2 protein from hepatitis C virus genotype 1b …
The Effects Of Herkinorin, The First Mu-Selective Ligand From A Salvinorin A-Derived Scaffold, In A Neuroendocrine Biomarker Assay In Nonhuman Primates, Eduardo R. Butelman, Szymon Rus, Denise S. Simpson, Angela Wolf, Thomas E. Prisinzano, Mary Jeanne Kreek
The Effects Of Herkinorin, The First Mu-Selective Ligand From A Salvinorin A-Derived Scaffold, In A Neuroendocrine Biomarker Assay In Nonhuman Primates, Eduardo R. Butelman, Szymon Rus, Denise S. Simpson, Angela Wolf, Thomas E. Prisinzano, Mary Jeanne Kreek
Pharmacy Faculty Publications
Herkinorin is the first μ-opioid receptor-selective ligand from the salvinorin A diterpenoid scaffold. Herkinorin has relative μ > κ > δ binding selectivity, and it can act as an agonist at both μ- and κ-receptors, in vitro. These studies were the first in vivo evaluation of the effects of herkinorin in nonhuman primates, using prolactin release, a neuroendocrine biomarker assay that is responsive to both μ- and κ-agonists, as well as to compounds with limited ability to cross the blood-brain barrier. In cumulative dosing studies (0.01–0.32 mg/kg i.v.), herkinorin produced only small effects in gonadally intact males (n = 4), but …
National Survey Of Introductory Pharmacy Practice Experience Programs, Patricia L. Darbishire, Trish Devine, Michael R. Holowatyj, Andrew N. Schmelz
National Survey Of Introductory Pharmacy Practice Experience Programs, Patricia L. Darbishire, Trish Devine, Michael R. Holowatyj, Andrew N. Schmelz
Scholarship and Professional Work – COPHS
Objective: The objective of this study was to identify commonalities and provide a descriptive overview of key program elements and oversight of U.S. introductory pharmacy practice experience (IPPE) programs.
Methods: A Web-based questionnaire, consisting of 40 questions, was sent to 91 schools of pharmacy. The questionnaire addressed the following IPPE program topics, in addition to school demographics: program oversight, experience site selection, program structure, relationship to didactic curriculum, and quality assurance issues.
Results: Forty-six schools of pharmacy responded resulting in a response rate of 50.5%. Results identified commonalities in each of the key areas, as well as identifying multiple discrepancies …
Sex Differences In Angiotensin Signaling In Bulbospinal Neurons In The Rat Rostral Ventrolateral Medulla, Gang Wang, Teresa A. Milner, Robert C. Speth, Andrea C. Gore, Di Wu, Costantino Iadecola, Joseph P. Pierce
Sex Differences In Angiotensin Signaling In Bulbospinal Neurons In The Rat Rostral Ventrolateral Medulla, Gang Wang, Teresa A. Milner, Robert C. Speth, Andrea C. Gore, Di Wu, Costantino Iadecola, Joseph P. Pierce
HPD Articles
Sex differences may play a significant role in determining the risk of hypertension. Bulbospinal neurons in the rostral ventrolateral medulla (RVLM) are involved in the tonic regulation of arterial pressure and participate in the central mechanisms of hypertension. Angiotensin II (ANG II) acting on angiotensin type 1 (AT(1)) receptors in RVLM neurons is implicated in the development of hypertension by activating NADPH oxidase and producing reactive oxygen species (ROS). Therefore, we analyzed RVLM bulbospinal neurons to determine whether there are sex differences in: 1) immunolabeling for AT(1) receptors and the key NADPH oxidase subunit p47 using dual-label immunoelectron microscopy, and …
Repression Of The Sumo-Specific Protease Senp1 Induces P53-Dependent Premature Senescence In Normal Human Fibroblasts, Kristin E. Yates, Gregory A. Korbel, Michael Shtutman, Igor Roninson, Daniel Dimaio
Repression Of The Sumo-Specific Protease Senp1 Induces P53-Dependent Premature Senescence In Normal Human Fibroblasts, Kristin E. Yates, Gregory A. Korbel, Michael Shtutman, Igor Roninson, Daniel Dimaio
Faculty Publications
The proliferative lifespan of normal somatic human cells in culture terminates in a permanent growth-arrested state known as replicative senescence. In this study, we show that RNA interference-mediated repression of the genes encoding the small ubiquitin-related modifier (SUMO)-specific proteases, Senp1, Senp2, and Senp7, induced low passage primary human fibroblasts to senesce rapidly. Following Senp1 repression, we observed a global increase in sumoylated proteins and in the number and size of nuclear SUMO-containing promyelocytic leukemia (PML) bodies. SUMO/PML bodies also increased during replicative senescence. p53 transcriptional activity was enhanced towards known p53 target genes following repression of Senp1, and inhibition of …
Method For Enhancing Protective Cellular Responses To Genotoxic Stress In Skin, Elaine L. Jacobson, Myron K. Jacobson
Method For Enhancing Protective Cellular Responses To Genotoxic Stress In Skin, Elaine L. Jacobson, Myron K. Jacobson
Pharmaceutical Sciences Faculty Patents
The present invention is directed to methods of using pro-NAD agents capable of enhancing the dermal and epidermal skin cell NAD content. These pro-NAD agents may be administered topically, orally, or parenterally to enhance DNA repair and other protective responses to DNA damage. The invention further relates to pharmaceutical compositions comprising pro-NAD agents that effectively elevate intracellular NAD content. Finally, the invention relates to the method of using the pro-NAD agents to treat disorders such as sunburn and other skin deterioration that results from DNA damage in skin cells.
Human Brain Contains A Novel Non-At1, Non-At2 Binding Site For Active Angiotensin Peptides, Vardan T. Karamyan, Craig A. Stockmeier, Robert C. Speth
Human Brain Contains A Novel Non-At1, Non-At2 Binding Site For Active Angiotensin Peptides, Vardan T. Karamyan, Craig A. Stockmeier, Robert C. Speth
HPD Articles
AIMS: To determine whether the novel non-AT1, non-AT2 binding site for angiotensins recently discovered in rodent brains occurs in the human brain. MAIN METHODS: Radioligand binding assays of (125)I-sarcosine(1), isoleucine(8) angiotensin II binding were carried out in homogenates of the rostral pole of the temporal cortex of human brains containing 0.3 mM parachloromercuribenzoate (PCMB), 10 microM losartan to saturate AT1 receptors, 10 microM PD123319 to saturate AT2 receptors, with or without 10 microM angiotensin II to define specific binding. Competition binding assays employed a variety of angiotensin peptides, specific angiotensin receptor antagonists, several neuropeptides and an endopeptidase inhibitor to determine …
Derivatives Of Mithramycin And Methods Of Making And Uses Thereof, Jürgen Rohr, Lily L. Remsing, Mohammad Ner-E-Alam, Jose A. Salas, Carmen Mendez, Alfredo F. Brana, Ana M. Gonzalez
Derivatives Of Mithramycin And Methods Of Making And Uses Thereof, Jürgen Rohr, Lily L. Remsing, Mohammad Ner-E-Alam, Jose A. Salas, Carmen Mendez, Alfredo F. Brana, Ana M. Gonzalez
Pharmaceutical Sciences Faculty Patents
The invention, in one aspect, generally relates to mithramycin derivatives from mutated Streptomyces argillaceus and their production. The invention also relates using the derivatives for the treatment of various diseases. Finally, the invention relates to a mutated Streptomyces argillaceus useful in the production of the mithramycin derivatives.
Neurovirulence Of Polytropic Murine Retrovirus Is Influenced By Two Separate Regions On Opposite Sides Of The Envelope Protein Receptor Binding Domain, Karin E. Peterson, Susan Pourciau, Min Du, Rachel Lacasse, Melissa Pathmajeyan, David J. Poulsen, Mavis Agbandje-Mckenna, Kathy Wehrly, Bruce Chesebro
Neurovirulence Of Polytropic Murine Retrovirus Is Influenced By Two Separate Regions On Opposite Sides Of The Envelope Protein Receptor Binding Domain, Karin E. Peterson, Susan Pourciau, Min Du, Rachel Lacasse, Melissa Pathmajeyan, David J. Poulsen, Mavis Agbandje-Mckenna, Kathy Wehrly, Bruce Chesebro
Biomedical and Pharmaceutical Sciences Faculty Publications
Changes in the envelope proteins of retroviruses can alter the ability of these viruses to infect the central nervous system (CNS) and induce neurological disease. In the present study, nine envelope residues were found to influence neurovirulence of the Friend murine polytropic retrovirus Fr98. When projected on a three-dimensional model, these residues were clustered in two spatially separated groups, one in variable region B of the receptor binding site and the other on the opposite side of the envelope. Further studies indicated a role for these residues in virus replication in the CNS, although the residues did not affect viral …
Identification Of Highly Expressed, Soluble Proteins Using An Improved, High-Throughput Pooled Orf Expression Technology, Timothy Waybright, William Gillette, Dominic Esposito, Robert Stephens, David Lucas, James Hartley, Timothy D. Veenstra
Identification Of Highly Expressed, Soluble Proteins Using An Improved, High-Throughput Pooled Orf Expression Technology, Timothy Waybright, William Gillette, Dominic Esposito, Robert Stephens, David Lucas, James Hartley, Timothy D. Veenstra
Pharmaceutical Sciences Faculty Publications
This article describes an improved pooled open reading frame (ORF) expression technology (POET) that uses recombinational cloning and solution-based tandem mass spectrometry (MS/MS) to identify ORFs that yield high levels of soluble, purified protein when expressed in Escherichia coli. Using this method, three identical pools of 512 human ORFs were subcloned, purified, and transfected into three separate E. coli cultures. After bulk expression and purification, the proteins from the three separate pools were digested into tryptic peptides. Each of these samples was subsequently analyzed in triplicate using reversed-phase high-performance liquid chromatography (LC) coupled directly online with MS/MS. The abundance of …
Fluid And Electrolytes Review, Jane Gervasio
Nutrition Support, Jane Gervasio
Pharmacy Newsletter : August 2008, Pharmacy Department
Pharmacy Newsletter : August 2008, Pharmacy Department
Pharmacy Newsletter
Content:
- Pharmacy News
- FDA Alerts
- Heparin: An update
- Warfarin interaction update
- Adverse drug reaction update: pethidine induced rashes