Medicine and Health Sciences Commons^™

P-Glycoprotein And Membrane Permeability As Determinants Of Xenobiotic Bioavailability And Bioaccumulation, Xiaobing Tan

LSU Doctoral Dissertations

The ABC transporter P-glycoprotein (Pgp) and membrane permeability are determinant factors for absorption and disposition of xenobiotics. These studies investigated the effects of potential modulators of Pgp and/or membrane function on the disposition, bioavailability and bioaccumulation of environmentally relevant pharmaceuticals, tetracycline and ivermectin, and the carcinogen benzo[a]pyrene in catfish. The pesticide dieldrin and surfactant linear alkylbenzene sulfonate (LAS) were selected as mixture components thought to have potential interactions with these determinants of disposition. Initial in situ experiments demonstrated dieldrin and tetracycline both significantly inhibited biliary excretion of Pgp prototypic substrate Rhodamine-123 in isolated perfused livers. Further, dieldrin (20µM) reduced movement …

Prevalence Of Family History Of Speech-Language Impairment In An African American Sample, Tricia Mccully Rodrigue

LSU Master's Theses

The purpose of this study was to examine the prevalence of a positive family history of speech and/or language impairments in an African American sample. The first phase of the study used questionnaires from the primary caregivers of 161 children. The questionnaires allowed for an examination of family history as a function of a child’s socioeconomic level (+/- high school level of maternal education), educational placement (+/- receipt of services by a speech language clinician), and clinical status (+/- profile consistent with a diagnosis of SLI). The second phase of the study included interviews that were collected from 17 families …

Lymphohistiocytic Proliferative Syndrome Of Alligators (Alligator Mississippiensis): A Cutaneous Manifestation Of West Nile Virus, Javier G. Nevarez

LSU Doctoral Dissertations

ABSTRACT In 1999, there were reports of a new type of lesion in the hides of captive reared alligators from Florida. Similar lesions were first reported from alligator hides in Louisiana in 2001; however, it wasn’t until 2002 that small epizootics became apparent. In 2002, the Louisiana Department of Wildlife and Fisheries began a collaborative effort with the Louisiana State University School of Veterinary Medicine (LSU SVM) to help elucidate the etiology of “PIX” disease, later renamed Lymphohistiocytic Proliferative Syndrome of Alligators (LPSA). Preliminary work concluded that LPSA was a systemic disease affecting multiple tissues. Based on the results of …

Role Of Heparanase And Heparanase-Degraded Heparan Sulfate In Brain-Metastatic Melanoma, Madhuchhanda Roy

LSU Doctoral Dissertations

Cancer metastasis is a frequent manifestation of malignant melanoma progression. Successful invasion into distant organs by tumor cells must include attachment to microvessel endothelial cells, and degradation of extracellular matrix. Heparan sulfate proteoglycans are ubiquitous macromolecules associated with cell surface and extracellular matrix of a wide range of cells and tissues. Heparanase is an extracellular matrix degradative enzyme which degrades the heparan sulfate chains of heparan sulfate proteoglycans. To investigate effects of changes in heparanase gene expression in metastatic melanoma cells, we constructed adenoviral vectors containing the full-length human heparanase cDNA in both sense (Ad-S/hep) and anti-sense orientations (Ad-AS/hep). We …

A Study Of Auxiliary Be In African American English: A Comparison Of Children With And Without Specific Language Impairment, April W. Garrity

LSU Doctoral Dissertations

This study’s purpose was to examine the use of auxiliary BE forms in African American English (AAE)-speaking children with and without language impairment. The impetus for this work was a lack of information in the literature about BE use in AAE as a function of form, language status, and tasks, and the relevance of this type of data for testing one theoretical model of childhood language impairment, the Extended Optional Infinitive account (EOI; Rice, Wexler, & Cleave, 1995). Thirty African Americans participated: 10 six-year-olds with specific language impairment (SLI); 10 age controls (AM); and, 10 language controls (LM). All of …

Depletion Of 32-Kbp Circular Plasmids From Borrelia Burgdorferi, Amanda Paige Derouen Polito

LSU Master's Theses

The Lyme disease spirochete Borrelia burgdorferi has a very unusual genome composed of one linear chromosome and up to 21 linear and circular plasmids. Several plasmids are known to be important either for mammalian infection or tick colonization. A single spirochete harbors up to 7 different cp32 plasmids; however, nothing is known about their role in mammalian infection. The plasmids in this family are well maintained during in vitro cultivation, making it difficult to study their functions. To effectively deplete the plasmids, an 8kbp fragment containing essential elements for replication and partitioning in B. burgdorferi was amplified from one of …

Characterization Of A Virulence Related Hypothetical Protein In Edwardsiella Ictaluri, Ildiko Katalin Polyak

LSU Master's Theses

Although the biochemical characterization of E. ictaluri, the subsequent disease progression of enteric septicemia of catfish (ESC), and the associated pathologic lesions are well characterized, the mechanism of invasion of E. ictaluri into a susceptible host is poorly understood. Identification and confirmation of virulence factors and associated genes of E. ictaluri is crucial to elucidating the pathogenesis of this important disease. A signature tagged mutagenesis (STM) study conducted by Thune et al. (2006) identified 50 E. ictaluri clones with transposon insertions in genes potentially involved in pathogenesis. A specific STM mutant, 233PR, carrying a transposon insertion in a gene encoding …

The Role Of Toll-Like Receptor 7 In The Neuropathogenesis Of Retrovirus Infection In Neonates, Stephanie Diane Lewis

LSU Master's Theses

Viral infections of the central nervous system (CNS) in infants are rare; however, they are associated with high morbidity and mortality rates. These virus infections often induce strong innate immune responses in the brain including: the production of cytokines and chemokines, the activation of astrocytes and microglia and the recruitment of macrophages. Innate immune responses are often initiated by toll-like receptors (TLR). Several studies have demonstrated that toll-like receptor 7 (TLR7) can be stimulated by single-stranded RNA from multiple viruses. In the current study, we examined the mechanism by which TLR7 contributes to neuroinflammation in the neonatal brain using a …

Tracing The Origin Of The Recent Rise In Neuropathogenic Ehv-1, Kathryn Laura Smith

University of Kentucky Master's Theses

Equine herpesvirus type-1(EHV-1) is a complex virus known for inducing various forms of disease in horses. In recent years, the number of cases of neurological disease caused by this virus has increased. While there are a number of possible sources for this recent surge, this project set out to determine if a genotypic shift in the latent population of the virus in favor of the neuropathogenic form of EHV-1 is the basis for the recent increase in frequency of EHV-1 neurologic disease. To ascertain if such a shift has in fact occurred, 450 EHV-1 isolates were obtained from fetal tissues …

Influence Of Tumor Necrosis Factor-Alpha And Minocycline On Microglia And Macrophage Activation During Polytropic Retrovirus Infection, Meryll E. Corbin

LSU Master's Theses

Microglia/macrophage activation has been associated with the pathogenesis of various neurological diseases including human immunodeficiency virus encephalitis, transmissible spongiform encephalitis, and Alzheimer's disease (AD). In vitro studies have indicated a role for TNFα in activating these cells which leads to their migration, proliferation, and secretion of proinflammatory cytokines and chemokines that may potentially damage brain tissue. In the current study, we analyzed the phenotype of microglia and macrophages enriched from wild type and TNFα deficient mice infected with a neurovirulent murine retrovirus. Although TNF receptors CD120a and CD120b were expressed on both microglia and macrophage population, unaltered by either retrovirus …

Expectation In Auditory Processing Of Environmental Sounds In People With Fluent Aphasia, Meghan Evelyn Collins

LSU Master's Theses

The purpose of this study was to examine the integrity of the nonverbal auditory system in subjects with fluent aphasia, and determine the relative preservation of the nonverbal auditory system in comparison to the lexical system. This was attempted through the task of expectation, a high level processing skill. Two groups of participants were examined: a group with fluent aphasia, and a group of non-neurologically damaged individuals. Participants were administered two nonverbal auditory conditions devoid of lexical information, a simple condition and a complex condition in which they were required to determine if the last sound heard in a sequence …

Exploration Of Computational Methods For Classification Of Movement Intention During Human Voluntary Movement From Single Trial Eeg, Ou Bai, Peter Lin, Sherry Vorbach, Jiang Li, Steve Furlani, Mark Hallett

Electrical & Computer Engineering Faculty Publications

Objective: To explore effective combinations of computational methods for the prediction of movement intention preceding the production of self-paced right and left hand movements from single trial scalp electroencephalogram (EEG).

Methods: Twelve naïve subjects performed self-paced movements consisting of three key strokes with either hand. EEG was recorded from 128 channels. The exploration was performed offline on single trial EEG data. We proposed that a successful computational procedure for classification would consist of spatial filtering, temporal filtering, feature selection, and pattern classification. A systematic investigation was performed with combinations of spatial filtering using principal component analysis (PCA), independent component analysis …

Using Pareto Fronts To Evaluate Polyp Detection Algorithms For Ct Colonography, Adam Huang, Jiang Li, Ronald M. Summers, Nicholas Petrick, Amy K. Hara

Electrical & Computer Engineering Faculty Publications

We evaluate and improve an existing curvature-based region growing algorithm for colonic polyp detection for our CT colonography (CTC) computer-aided detection (CAD) system by using Pareto fronts. The performance of a polyp detection algorithm involves two conflicting objectives, minimizing both false negative (FN) and false positive (FP) detection rates. This problem does not produce a single optimal solution but a set of solutions known as a Pareto front. Any solution in a Pareto front can only outperform other solutions in one of the two competing objectives. Using evolutionary algorithms to find the Pareto fronts for multi-objective optimization problems has been …

Validating Pareto Optimal Operation Parameters Of Polyp Detection Algorithms For Ct Colonography, Jiang Li, Adam Huang, Nicholas Petrick, Jianhua Yao, Ronald M. Summers, Maryellen L. Giger (Ed.), Nico Karssemeijer (Ed.)

Electrical & Computer Engineering Faculty Publications

We evaluated a Pareto front-based multi-objective evolutionary algorithm for optimizing our CT colonography (CTC) computer-aided detection (CAD) system. The system identifies colonic polyps based on curvature and volumetric based features, where a set of thresholds for these features was optimized by the evolutionary algorithm. We utilized a two-fold cross-validation (CV) method to test if the optimized thresholds can be generalized to new data sets. We performed the CV method on 133 patients; each patient had a prone and a supine scan. There were 103 colonoscopically confirmed polyps resulting in 188 positive detections in CTC reading from either the prone or …

Responses Of Respiratory System Cells In Vitro And In Vivo To Petrochemical Combustion-Derived Ultrafine Particles, Gleeson Murphy, Jr.

LSU Doctoral Dissertations

Environmental contamination with airborne particles has been a human health concern for many years. Epidemiologic studies in urban communities have linked ambient particle exposure to various health effects, including chronic obstructive pulmonary disease, lung cancer, and several cardiovascular disease conditions. The pathogenesis of these conditions with respect to ambient particle exposure is complex because ambient particles are complex in composition. The particles vary greatly in origin, size, surface area, and elemental composition; and a given particle type, such as those generated by petrochemical (gasoline, diesel, industrial substrate) combustion, may be coated with many other compounds, including polynuclear aromatic hydrocarbons (PAHs). …

Genetics Of Herpes Simplex Virus Type-1 Tegument Proteins Involved In Virion Morphogenesis And Egress, Preston A. Fulmer

LSU Doctoral Dissertations

Herpes simplex virus type-1 (HSV-1) morphogenesis occurs in multiple stages within infected cells. Initially, the virion capsid assembles within the nucleus and buds through the nuclear membrane into the cytoplasm. Within the cytoplasm, additional tegument proteins attach to the capsid and the fully tegumented capsids bud into trans-Golgi network (TGN) derived vesicles. Enveloped virions are ultimately secreted to extracellular spaces. The process by which the cytoplasmic capsids bud into TGN-derived vesicles is not well understood. The prevalent model calls for specific interactions among viral tegument proteins and membrane proteins and glycoproteins embedded within TGN membranes. To further investigate the roles …

Evaluation Of Selected Immune Response To Haemonchus Contortus In Gulf Coast Native Compared To Suffolk Lambs, Krishna P. Shakya

LSU Doctoral Dissertations

Haemonchus contortus is one of the major nematode parasites causing substantial economic losses in small ruminant farming worldwide. Recently, effect of anthelmintic treatment has decreased due to an increasing problem of nematode populations that have developed resistance to anthelmintics. There are certain breeds of sheep that are identified as being relatively resistant to the parasite including Gulf Coast Native (Native) sheep. Understanding the mode of immune response that helps these breeds of sheep control infection could help design vaccines and enhance control programs. This experiment was designed to evaluate the immunological responses of Native, compared with susceptible Suffolk sheep that …

Effects Of The Menstrual Cycle On The Vibratory Characteristics Of The Vocal Folds, Aimee Michelle Bonnette

LSU Master's Theses

The purpose of this study was to obtain preliminary data on several factors that may influence the vibratory patterns of the vocal folds in addition to the hormonal fluctuations present during the menstrual cycle. These factors included patient reports of severity of reflux symptoms, severity of premenstrual symptoms, and severity of negative vocal hygiene behaviors prior to each evaluation. Ten subjects who did not experience complaints of their voice participated in the study. Data from four subjects who were not on birth control and four on birth control were analyzed for the first cycle, which included self-perception, acoustic analysis, and …

Effect Of Adapted Phonic Faces Story Books On Phonological Skills Of Children With Severe Expressive Language Disorders, Meher Hormazd Banajee

LSU Doctoral Dissertations

Although No Child Left Behind (2001) encourages the inclusion of all children within the regular curriculum, children with severe speech and physical disabilities rarely are provided with the literacy education provided to grade-level peers. This study taught alphabetic skills to three children with severe speech and physical impairments in the context of traditional alphabet books versus Phonic Faces Storybooks. Both storybooks were converted into e-books using Microsoft Office PowerPoint (Microsoft Office, 2003) and accessed using a single rocking lever switch. Two graphemes were selected based on incorrect responses from the pretest administration of the The Phonological Awareness Test (Roberson & …

Accident Denominators Relative To Age Groups In Heavy Industries Of The Port Hedland Region Of Western Australia, John R. Bottrell

Theses: Doctorates and Masters

The aim of this research is to investigate characteristics of accident denominators across age groups in mining and associated process industries in the Port Hedland region of Western Australia. Emphasis has been focussed on comparing young, inexperienced groups with older, more experienced groups. A literature review revealed some key contributors to accidents among younger workers, in particular, those who had only recently entered the workforce. The review also revealed contributors impacting accidents regarding other age groups over a wide range of industry types. From these findings an accident construct model and questionnaire were designed to identify contributing and mitigating denominators …

The Effect Of Concurrent Resistance And Endurance Training On Physiological And Performance Parameters Of Well Trained Endurance Cyclists, Gregory T. Levin

Theses: Doctorates and Masters

Purpose: To investigate the effect of concurrent resistance and cycle training on the physiological and performance characteristics of well trained cyclists. Secondly, this study aimed to investigate the reliability of a new cycling time-trial test that incorporated repeated high-intensity sprint segments, both prior to and after a training intervention, with well trained cyclists.

Is The Treatment And Transport Of Asystolic Cardiac Arrest Patients To Hospital By Ambulance Services Appropriate?, Andy Symons

Theses: Doctorates and Masters

The Paramedic's role requires them to attend many patients in cardiac arrest. The victim' s survival depends on a variety of issues, such as previous medical history, pathological cause of cardiac arrest, presenting cardiac arrest dysrhythmias, and the time elapsed before medical aid is administered. The purpose of this present study was to review current practice advocated by St John Ambulance Service WA for the treatment of the cardiac arrest dysrhythmia `asystole'. At present, certain victims of an asystolic arrest are treated and transported to hospital irrespective of time in arrest. As such, questions are raised as to the suitability …

The Endogenous Cannabinoid Anandamide Inhibits Cromakalim-Activated K+ Currents In Follicle-Enclosed Xenopus Oocytes, Murat Oz, Keun-Hang Susan Yang, Meral Dinc, Toni S. Shippenberg

Mathematics, Physics, and Computer Science Faculty Articles and Research

The effect of the endogenous cannabinoid anandamide on K+ currents activated by the ATP-sensitive potassium (KATP) channel opener cromakalim was investigated in follicle-enclosed Xenopus oocytes using the two-electrode voltage-clamp technique. Anandamide (1–90 μM) reversibly inhibited cromakalim-induced K+ currents, with an IC50 value of 8.1 ± 2 μM. Inhibition was noncompetitive and independent of membrane potential. Coapplication of anandamide with the cannabinoid type 1 (CB1) receptor antagonist N-(piperidin-1-yl)-5-(4-chlorophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboximide hydrochloride (SR 141716A) (1 μM), the CB2 receptor antagonist N-[(1S)endo-1,3,3-trimethyl bicyclo heptan-2-yl]-5-(4-chloro-3-methylphenyl)-1-(4-methylbenzyl)-pyrazole-3-carboxamide (SR144528) (1 μM), or pertussis toxin (5 μg/ml) did not alter the inhibitory effect of anandamide, suggesting that known cannabinoid receptors …

Effects Of Methylprednisolone And Its Liver-Targeted Dextran Prodrug On Ischemia–Reperfusion Injury In A Rat Liver Transplantation Model, Anjaneya Chimalakonda, Reza Mehvar

Pharmacy Faculty Articles and Research

Purpose. To evaluate the effectiveness of a liver-targeted dextran prodrug (DMP) of methylprednisolone ( MP) in cold preservation-warm reperfusion injury associated with liver transplantation.

Methods. The effects of donor pretreatment with single 5 mg/kg doses of MP or DMP on ischemia reperfusion damage to the liver were studied after 8 or 24 h of cold preservation in both isolated perfused rat liver (IPRL) and syngeneic orthotopic rat liver transplantation (OLT) models.

Results. In IPRL studies, donor pretreatment with DMP, and to a lesser degree MP, significantly improved the uptake of hyaluronic acid (HA), a marker of endothelial …

Electromyographic Data Do Not Support A Progressive Recruitment Of Muscle Fibers During Exercise Exhibiting A Vo2 Slow Component, Daniel T. Cannon, Fred W. Kolkhorst, Daniel Cipriani

Physical Therapy Faculty Articles and Research

The origin of the slow component (SC) of oxygen uptake kinetics, presenting during exercise above the ventilatory threshold (VT), remains unclear. Possible physiologic mechanisms include a progressive recruitment of type II muscle fibers. The purpose of this study was to examine alterations in muscle activity through electromyography (EMG) and mean power frequency (MPF) analysis during heavy cycling exercise. Eight trained cyclists (mean±S.E.; age=30±3 years, height=177±4 cm, weight=73.8±6.5 kg, VO2max=4.33±0.28lmin−1) completed transitions from 20 W to a workload equaling 50% of the difference between VT and VO2max. VO2 was monitored using a breath-by-breath measurement system, and EMG data were gathered from …

Flavin Charge Transfer Transitions Assist Dna Photolyase Electron Transfer, Spiros S. Skourtis, Tatiana Prytkova, David Beratan

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

This contribution describes molecular dynamics, semi-empirical and ab-initio studies of the primary photo-induced electron transfer reaction in DNA photolyase. DNA photolyases are FADH−-containing proteins that repair UV-damaged DNA by photo-induced electron transfer. A DNA photolyase recognizes and binds to cyclobutatne pyrimidine dimer lesions of DNA. The protein repairs a bound lesion by transferring an electron to the lesion from FADH−, upon photo-excitation of FADH− with 350–450 nm light. We compute the lowest singlet excited states of FADH− in DNA photolyase using INDO/S configuration interaction, time-dependent density-functional, and time-dependent Hartree-Fock methods. The calculations identify the lowest singlet excited state of FADH− …

[Pain In The Elderly: Prevalence And Associated Factors] Dolor En Adultos Mayores De 50 Años: Prevalencia Y Factores Asociados, Abel Jesús Barragán-Berlanga, Silvia Mejia-Arango, Luis Miguel Gutiérrez-Robledo

School of Medicine Publications

OBJECTIVE: To determine the prevalence and the factors associated with pain in the Mexican elderly.

MATERIAL AND METHODS: Persons ages 50 years or older answered the question: "Do you often suffer physical pain?" Prevalences were obtained, after which multivariate analyses were conducted for the entire sample and for each age group to determine the associated factors.

RESULTS: Pain prevalence was 41.5%, more frequent in women (48.3% vs. 33.6%) and increased with age. There was a lower prevalence for persons with more education. Pain was directly associated with the report of arthritis, lung disease, falls, hypertension, depression, stroke, cancer history and …

Prevalence Of Self-Reported Overweight-Obesity And Its Association With Socioeconomic And Health Factors Among Older Mexican Adults, Liliana Ruiz-Arregui, Lilia Castillo-Martínez, Arturo Orea-Tejeda, Silvia Mejia-Arango Dr.

School of Medicine Publications

Objective: To investigate the prevalence of obesity and its association with socioeconomic factors and comorbidities in a population-based study.

Material and methods: Data were examined from 4,605 persons ages 60 and older that participated in the 2001 Mexican Health and Aging Study, conducted in rural and urban communities in Mexico. The prevalence of obesity (according to self-reported weight and height) was obtained, stratified by age, and logistic regression was used to study cross-sectional associations between obesity and socioeconomic factors.

Results: Of the population studied, 20.9% were classified as obese and the prevalence diminishes with age. Overall, women were more likely …

The Pet Food Recall Puzzle: Who, What, Why, And How Much, Roxanne Greitz Miller

Education Faculty Articles and Research

Last spring, North America was gripped in the largest pet food recall in history. News outlets reported tens of thousands of dogs and cats becoming ill, and many dying, as a result of ingesting contaminated pet food. Several pet owners have filed lawsuits against the manufacturers and distributors of the pet food products. I think this is an excellent real-life science story to use with students to show science investigation in action, and to discuss the intersections between the role of regulatory agencies and the public.

In order to understand the case, you first have to know the facts. We'll …

Chronic Care At The Crossroads: Exploring Solutions For Chronic Care Management. Report On The Us Summit., Janice L Clarke

College of Population Health Faculty Papers

Report on the US Summit