Medicine and Health Sciences Commons^™

Moment Analysis Of Near-Equilibrium Binding Interactions During Electrophoresis, Yousef Daneshbod, James D. Sterling, Ali Nadim

Business and Information Technology Faculty Research & Creative Works

The electrophoretic transport of three chemically reacting species, two of which can bind reversibly to form the third, is analyzed mathematically. The species are assumed to move horizontally through a long channel with different electrophoretic mobilities and diffusion coefficients. By considering small perturbations of the system about equilibrium or when one of the two binding species is much more abundant than the other, the governing advection-reaction- diffusion equations can be linearized and studied via the method of moments. The result is a set of coupled ordinary differential equations for the moments that can be solved analytically. Analysis of the long-time …

The Association Of Complex Liver Disorders With Hbv Genotypes Prevalent In Pakistan, Saeeda Baig, Anwar Ali Siddiqui, Waqaruddin Ahmed, Huma Qureshi, Ambreen Arif

Department of Biological & Biomedical Sciences

Background

Genotyping of HBV is generally used for determining the epidemiological relationship between various virus strains and origin of infection mostly in research studies. The utility of genotyping for clinical applications is only beginning to gain importance. Whether HBV genotyping will constitute part of the clinical evaluation of Hepatitis B patients depends largely on the availability of the relevance of the evidence based information. Since Pakistan has a HBV genotype distribution which has been considered less virulent as investigated by earlier studies from south East Asian countries, a study on correlation between HBV genotypes and risk of progression to further …

Methods Of Delaying Development Of Cea-Associated Tumors Using Anti-Idiotype Antibody 3h1, Malaya Chatterjee, Kenneth A. Foon, Sunil K. Chatterjee

Pharmaceutical Sciences Faculty Patents

The present invention provides methods of delaying development of CEA-associated tumors using the anti-idiotype antibody 3H1, particularly in high-risk individuals.

The Fine Line Of Perfectionism: Is It A Strength Or A Weakness In The Workplace?, Lindsay Bousman

Department of Psychology: Dissertations, Theses, and Student Research

Perfectionism has been traditionally researched in the clinical psychology domain. While some research has used a normal student population, research applying perfectionism theories to a normal adult population working in Corporate America has not been conducted. Current research suggests two distinct types of perfectionism, maladaptive and adaptive, with different consequences. In this research, maladaptive and adaptive perfectionism were used to determine that traditional perfectionism measures can be used with a working adult sample to achieve similar psychometric properties, and to preliminarily test hypotheses related to their relationship with other individual difference variables. Second, maladaptive and adaptive perfectionism were used to …

A Dipeptidyl Aminopeptidase-Like Protein Remodels Gating Charge Dynamics In Kv4.2 Channels, Kevin Dougherty, Manuel Covarrubias

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Dipeptidyl aminopeptidase–like proteins (DPLPs) interact with Kv4 channels and thereby induce a profound remodeling of activation and inactivation gating. DPLPs are constitutive components of the neuronal Kv4 channel complex, and recent observations have suggested the critical functional role of the single transmembrane segment of these proteins (Zagha, E., A. Ozaita, S.Y. Chang, M.S. Nadal, U. Lin, M.J. Saganich, T. McCormack, K.O. Akinsanya, S.Y. Qi, and B. Rudy. 2005. J. Biol. Chem. 280:18853–18861). However, the underlying mechanism of action is unknown. We hypothesized that a unique interaction between the Kv4.2 channel and a DPLP found in brain (DPPX-S) may remodel the …

Conferred Immunity Against The H1n1 Type A Influenza Virus In Madin-Darby Canine Kidney Cells Via Cecropin B Transfection Therapy., Tomasz P. Stryjewski

Honors Capstones

No abstract provided.

Membranous Dysmenorrhea: A Case Series, Hatim A. Omar, Shawn J. Smith

Pediatrics Faculty Publications

The purpose was to illustrate the variability of hormonal contraception of patients that presented with membranous dysmenorrheal. A case analysis chart review was completed on six patients referred to a Pediatric Gynecologist in an academic setting. In each case the patient underwent a thorough pelvic and bimanual exam. Following the initial presentation, each patient continued to be followed on a regular visits. Cases: Two were using the transdermal contraceptive patch and oral contraceptive, but following the expulsion of decidual cast, they were both placed on depot medroxyprogesterone acetate (DMPA) without further complications. Three of the six cases were on DMPA …

Nonsexual Transmission Of Anogenital Warts In Children: A Retrospective Analysis, Valerie Jones, Shawn J. Smith, Hatim A. Omar

Pediatrics Faculty Publications

The purpose was to evaluate the prevalence of sexual abuse in patients who were referred to a pediatric gynecologist for evaluation based on the clinical findings of anogenital warts. A retrospective analysis was performed on 131 patients between the ages 6 month and 9 years referred to a pediatric gynecologist after the finding of anogenital warts by a clinical provider, parent or caregiver. A complete physical examination under colposcopy by a the same, trained pediatric gynecologist was completed, and a complete medical and family history including maternal and sibling history for evidence of Human Papillomavirus (HPV) and anogenital warts. The …

Ada News - 11/19/2007, American Dental Association, Publishing Division

ADA News

Established in 1970 as the official newspaper of the American Dental Association, the ADA News serves practicing dentists and others allied to the dental profession in the U.S. and internationally. It is the No. 1 source of news and information about the many benefits and services the ADA delivers to members daily as well as timely information on scientific, social, political and economic developments affecting dentistry and health care.

Effects Of Delayed Auditory Feedback On The Bereitschaftspotential, Jennifer L. Johnson

Theses and Dissertations

This study examined the brain electrical activity of normal speakers in a non-delayed auditory feedback (DAF) condition and when experiencing DAF to determine the effect DAF would have on the Bereitschaftspotential (BP). The BP reflects the preparatory state of a person prior to motor execution of an act and can be observed 1500 to 500 ms prior to voluntary movement. The participants in the study included 10 adults with normal speech. Each read a series of 30 sentences, both without DAF and with DAF, while the BP was measured. Results indicate that the BP is present across the scalp in …

Site-1 Protease Is Essential For Endochondral Bone Formation In Mice, Debabrata Patra, Xiaoyun Xing, Sherri Davies, Jennifer Bryan, Carl Franz, Ernst B. Hunziker, Linda J. Sandell

Open Access Publications

No abstract provided.

Animal Health Matters, Russ Daly, Jane Hennings

Animal Health MATTERS Newsletter

[Page] 1- New Diagnostic/Research Faculty Bring Talents to SDSU, Dr. Diego Del, Dr. Joy Scaria [Page] 1- More New Hires to Start Soon at VBS Department [Page] 2- Director's Message: “One Health” and the Importance of Training the Next Generation of Scientists [Page] 3- Summary of BVDV-PI Testing at SDSU ADRDL [Page] 4- The Clinical Pathology Section at SDSU’s ADRDL [Page] 5- Governor Daugaard Pays Visit to the ADRDL Through Stethoscope Ceremony [Page] 6- New Hire: Dana Rausch, Molecular Diagnostics [Page] 6- ADRDL Bacteriologist Serves as President [Page] 6- Serology Staff Member Aids Custer State Park Bison Testing Efforts [Page] …

Discovery Of Platelet-Type 12-Human Lipoxygenase Selective Inhibitors By High-Throughput Screening Of Structurally Diverse Libraries., Joshua D. Deschamps, Jeffrey T. Gautschi, Stephanie Whitman, Tyler A. Johnson, Nadine C. Gassner, Phillip Crews, Theodore R. Holman

Natural Sciences and Mathematics | Faculty Scholarship

Human lipoxygenases (hLO) have been implicated in a variety of diseases and cancers and each hLO isozyme appears to have distinct roles in cellular biology. This fact emphasizes the need for discovering selective hLO inhibitors for both understanding the role of specific lipoxygenases in the cell and developing pharmaceutical therapeutics. To this end, we have modified a known lipoxygenase assay for high-throughput (HTP) screening of both the National Cancer Institute (NCI) and the UC Santa Cruz marine extract library (UCSC-MEL) in search of platelet-type 12-hLO (12-hLO) selective inhibitors. The HTP screen led to the characterization of five novel 12-hLO inhibitors …

Adaptive Radiotherapy Planning On Decreasing Gross Tumor Volumes As Seen On Megavoltage Computed Tomography Images., Curtis Woodford, Slav Yartsev, R. Dar, Glenn Bauman, Jacob Van Dyk

Oncology Publications

PURPOSE: To evaluate gross tumor volume (GTV) changes for patients with non-small-cell lung cancer by using daily megavoltage (MV) computed tomography (CT) studies acquired before each treatment fraction on helical tomotherapy and to relate the potential benefit of adaptive image-guided radiotherapy to changes in GTV.

METHODS AND MATERIALS: Seventeen patients were prescribed 30 fractions of radiotherapy on helical tomotherapy for non-small-cell lung cancer at London Regional Cancer Program from Dec 2005 to March 2007. The GTV was contoured on the daily MVCT studies of each patient. Adapted plans were created using merged MVCT-kilovoltage CT image sets to investigate the advantages …

Split-Half Reliability Of Mlu And Mlu2 In Two Methods Of Utterance Segmentation, Alyse Diana Kemeny

Theses and Dissertations

Concerns regarding Mean Length of Utterance (MLU) has led to adaptations of this method of analysis. A recent study by Johnston (2001) introduced an alternative to MLU called MLU2. The current study investigates the split-half reliability of MLU and MLU2 as well as another alternative, Median Length of Utterance (Med-LU). Split-half reliability was found for these methods when segmented into Phonological and Communication Units. Split-half reliability of MLU2 was generally higher than that of MLU, and both were higher than Med-LU. The study suggests that MLU2 may also be a valuable tool for clinicians in analyzing child language.

Wingless Activity In The Precursor Cells Specifies Neuronal Migratory Behavior In The Drosophila Nerve Cord, Krishna Moorthi Bhat

Faculty, Staff and Student Publications

Neurons and their precursor cells are formed in different regions within the developing CNS, but they migrate and occupy very specific sites in the mature CNS. The ultimate position of neurons is crucial for establishing proper synaptic connectivity in the brain. In Drosophila, despite its extensive use as a model system to study neurogenesis, we know almost nothing about neuronal migration or its regulation. In this paper, I show that one of the most studied neuronal pairs in the Drosophila nerve cord, RP2/sib, has a complicated migratory route. Based on my studies on Wingless (Wg) signaling, I report that the …

Psychometrically Equivalent Cantonese Bisyllabic Word Recognition Materials Spoken By Male And Female Talkers, Brooke Kristin Conklin

Theses and Dissertations

The purpose of this study was to create psychometrically equivalent word lists in the language of Cantonese for word recognition testing. Frequently used bisyllabic Cantonese words were recorded by a native female and male talker. The word lists were evaluated by administering the word recognition lists to 20 native speakers of Cantonese with normal hearing. Each list was presented at 10 different intensity levels ranging from -5 to 40 dB HL in 5 dB increments. Logistic regression was used to determine the words with the steepest logistic regression slopes. The 200 words with the steepest slopes were then formulated into …

Leptospira Interrogans Endostatin-Like Outer Membrane Proteins Bind Host Fibronectin, Laminin And Regulators Of Complement, Brian Stevenson, Henry A. Choy, Marija Pinne, Matthew L. Rotondi, M. Clarke Miller, Edward Demoll, Peter Kraiczy, Anne E. Cooley, Trevor P. Creamer, Marc A. Suchard, Catherine A. Brissette, Ashutosh Verma, David A. Haake

Microbiology, Immunology, and Molecular Genetics Faculty Publications

The pathogenic spirochete Leptospira interrogans disseminates throughout its hosts via the bloodstream, then invades and colonizes a variety of host tissues. Infectious leptospires are resistant to killing by their hosts' alternative pathway of complement-mediated killing, and interact with various host extracellular matrix (ECM) components. The LenA outer surface protein (formerly called LfhA and Lsa24) was previously shown to bind the host ECM component laminin and the complement regulators factor H and factor H-related protein-1. We now demonstrate that infectious L. interrogans contain five additional paralogs of lenA, which we designated lenB, lenC, lenD, lenE and lenF …

Micro Rna 145 Targets The Insulin Receptor Substrate-1 And Inhibits The Growth Of Colon Cancer Cells, Bin Shi, Laura Sepp-Lorenzino, Marco Prisco, Peter Linsley, Tiziana Deangelis, Renato Baserga

Department of Cancer Biology Faculty Papers

The insulin receptor substrate-1 (IRS-1), a docking protein for both the type 1 insulin-like growth factor receptor (IGF-IR) and the insulin receptor, is known to send a mitogenic, anti-apoptotic, and anti-differentiation signal. Several micro RNAs (miRs) are suggested by the data base as possible candidates for targeting IRS-1. We show here that one of the miRs predicted by the data base, miR145, whether transfected as a synthetic oligonucleotide or expressed from a plasmid, causes down-regulation of IRS-1 in human colon cancer cells. IRS-1 mRNA is not decreased by miR145, while it is down-regulated by an siRNA targeting IRS-1. Targeting of …

Allelic Heterogeneity At The Equine Kit Locus In Dominant White (W) Horses, Bianca Haase, Samantha A. Brooks, Angela Schlumbaum, Pedro J. Azor, Ernest Bailey, Ferial Alaeddine, Meike Mevissen, Dominik Burger, Pierre-André Poncet, Stefan Rieder, Tosso Leeb

Veterinary Science Faculty Publications

White coat color has been a highly valued trait in horses for at least 2,000 years. Dominant white (W) is one of several known depigmentation phenotypes in horses. It shows considerable phenotypic variation, ranging from approximately 50% depigmented areas up to a completely white coat. In the horse, the four depigmentation phenotypes roan, sabino, tobiano, and dominant white were independently mapped to a chromosomal region on ECA 3 harboring the KIT gene. KIT plays an important role in melanoblast survival during embryonic development. We determined the sequence and genomic organization of the approximately 82 kb equine KIT gene. A mutation …

Ua12/2/1 College Heights Herald, Vol. 83, No. 20, Wku Student Affairs

WKU Administration Documents

WKU campus newspaper reporting campus, athletic and Bowling Green, Kentucky news.

Ua12/2/1 College Heights Herald, Vol. 83, No. 19, Wku Student Affairs

WKU Administration Documents

WKU campus newspaper reporting campus, athletic and Bowling Green, Kentucky news

Transcriptional Control Of Vascular Smooth Muscle Cell Proliferation By Peroxisome Proliferator-Activated Receptor-Γ: Therapeutic Implications For Cardiovascular Diseases, Florence Gizard, Dennis Bruemmer

Internal Medicine Faculty Publications

Proliferation of vascular smooth muscle cells (SMCs) is a critical process for the development of atherosclerosis and complications of procedures used to treat atherosclerotic diseases, including postangioplasty restenosis, vein graft failure, and transplant vasculopathy. Peroxisome proliferator-activated receptor (PPAR) gamma is a member of the nuclear hormone receptor superfamily and the molecular target for the thiazolidinediones (TZD), used clinically to treat insulin resistance in patients with type 2 diabetes. In addition to their efficacy to improve insulin sensitivity, TZD exert a broad spectrum of pleiotropic beneficial effects on vascular gene expression programs. In SMCs, PPARgamma is prominently upregulated during neointima formation …

Ada News - 11/05/2007, American Dental Association, Publishing Division

ADA News

Established in 1970 as the official newspaper of the American Dental Association, the ADA News serves practicing dentists and others allied to the dental profession in the U.S. and internationally. It is the No. 1 source of news and information about the many benefits and services the ADA delivers to members daily as well as timely information on scientific, social, political and economic developments affecting dentistry and health care.

Anti-Tumor Effect Of Doxycycline On Glioblastoma Cells, Andrea Wang-Gillam, Eric Siegel, Debra A. Mayes, Laura F. Hutchins, Yi-Hong Zhou

Neuroscience, Cell Biology & Physiology Faculty Publications

AIM: Glioblastoma multiforme (GBM) is the most common primary brain tumor in humans, and it is highly invasive. Doxycycline, first identified as an antimicrobial agent, is a nonspecific inhibitor of matrix metalloproteinases (MMPs). Our objective was to investigate the anti-MMP effect of doxycycline at therapeutically acceptable levels on glioma cells in vitro.

METHODS: The MTT assay was used to determine the anti-proliferative effects of doxycycline. MMP2 activity and expression were determined by gelatinase zymography and real-time quantitative RT-PCR, respectively. Cell invasion was assessed by Matrigel invasion assay.

RESULTS: Doxycycline exerted mild anti-proliferative effects on all three glioma cell lines …

Understanding Barriers For Adherence To Follow-Up Care For Abnormal Pap Tests, Katherine S. Eggleston, Ann L. Coker, Irene Prabhu Das, Suzanne T. Cordray, Kathryn J. Luchok

CRVAW Faculty Journal Articles

Objective: Approximately 4000 women annually will die from preventable and treatable cervical cancer. Failure to adhere to follow-up recommendations after an abnormal Pap test can lead to development of cervical cancer. This paper summarizes the body of literature on adherence to follow-up after an abnormal Pap test in order to facilitate development of interventions

to decrease morbidity and mortality due to cervical cancer.

Methods: We conducted a comprehensive search of published literature addressing risk factors for adherence or interventions to improve adherence following an abnormal Pap test as the outcome. We included peer-reviewed original research conducted in the …

Clinic Variation In The Cost-Effectiveness Of Contingency Management, Todd A. Olmstead, Nancy M. Petry

UCHC Articles - Research

This study determined whether, and by how much, the cost-effectiveness of contingency management (CM) varied across the eight clinics in the National Institute on Drug Abuse Clinical Trials Network MIEDAR trial. Incremental costs, incremental outcomes, and incremental costeffectiveness ratios (ICERs) of CM compared to usual care were calculated, compared and contrasted for each of the clinics. Results showed that the incremental cost of using CM compared to usual care varied by a factor of 1.9 across the clinics, ranging from an additional $306 to an additional $582 per patient. The effect of CM on the longest duration of continuous stimulant …

Variation In Gabra2 Predicts Drinking Behavior In Project Match Subjects, Lance O. Bauer, Jonathan Covault, Ofer Harel, Sourish Das, Henry R. Kranzler

UCHC Articles - Research

Background Previous studies have demonstrated, and replicated, an association between single nucleotide polymorphisms (SNPs) within the GABRA2 gene and risk for alcohol dependence. The present study examines the association of a GABRA2 SNP with another definition of alcohol involvement and with the effects of psychosocial treatment. Methods European-American subjects (n=812, 73.4% male) provided DNA samples for the analysis. All were participants in Project MATCH, a multi-center randomized clinical trial evaluating the efficacy of three types of psychosocial treatment for alcoholism: Cognitive Behavioral Therapy (CBT), Motivational Enhancement Therapy (MET), or Twelve Step Facilitation (TSF). The daily probabilities of drinking and heavy …

Xml Encoding Of Features Describing Rule-Based Modeling Of Reaction Networks With Multi-Component Molecular Complexes, Michael L. Blinov, Ion I. Moraru

UCHC Articles - Research

Multi-state molecules and multi-component complexes are commonly involved in cellular signaling. Accounting for molecules that have multiple potential states, such as a protein that may be phosphorylated on multiple residues, and molecules that combine to form heterogeneous complexes located among multiple compartments, generates an effect of combinatorial complexity. Models involving relatively few signaling molecules can include thousands of distinct chemical species. Several software tools (StochSim, BioNetGen) are already available to deal with combinatorial complexity. Such tools need information standards if models are to be shared, jointly evaluated and developed. Here we discuss XML conventions that can be adopted for modeling …

Medical Education Digest, Vol. 9 No. 6 (November/December 2007), Nova Southeastern University

Medical Education Digest

No abstract provided.