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2002

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Articles 1711 - 1740 of 1816

Full-Text Articles in Medicine and Health Sciences

Thymic Nurse Cells Determined In Situ At The Light And Electron Microscopic Level, F. Fi̇gen Kaymaz, Atti̇la Dağdevi̇ren Jan 2002

Thymic Nurse Cells Determined In Situ At The Light And Electron Microscopic Level, F. Fi̇gen Kaymaz, Atti̇la Dağdevi̇ren

Turkish Journal of Veterinary & Animal Sciences

Thymic nurse cells are isolated thymic epithelial/thymocyte complexes described in vitro. Their in vivo existence is controversial. We examined rat thymi in order to distinguish thymic nurse cells in vivo using the zinc iodide-osmium tetroxide (ZIO) fixation/staining technique. There were mainly two types of strongly ZIO reactive cells in the thymic cortex: macrophages and small clusters of thymic epithelial cells. The latter group was composed of thymic epithelial cells and intact thymocytes forming complexes. Thus we suggest that these structures are in situ equivalents of thymic nurse cells.


Detection And Rflp Analysis Of Canine Parvovirus (Cpv) Dna By Polymerase Chain Reaction (Pcr) In A Dog, Aykut Özkul, İhsan Keleş, Taner Karaoğlu, Mehmet Çabalar, İbrahi̇m Burgu Jan 2002

Detection And Rflp Analysis Of Canine Parvovirus (Cpv) Dna By Polymerase Chain Reaction (Pcr) In A Dog, Aykut Özkul, İhsan Keleş, Taner Karaoğlu, Mehmet Çabalar, İbrahi̇m Burgu

Turkish Journal of Veterinary & Animal Sciences

In this study, the detection of canine parvovirus (CPV) in a fecal sample from a dog with enteritis was performed for the first time using the polymerase chain reaction (PCR) in Turkey. The final PCR product was analyzed using the restriction fragment length polymorphysm (RFLP) technique. RFLP analysis using Apa LI and Eco RV restriction endonucleases revealed homology in the nucleotide sequence in at least the VP2 coding region of the virus DNAs detected in the fecal specimen and prepared from attenuated vaccine virus as a positive control.


Histopathology Of The Epidermal Papilloma In Cage Cultured Mirror Carp (Cyprinus Carpio) In Eğirdir Lake, Öznur Di̇ler, M. Rüştü Özen Jan 2002

Histopathology Of The Epidermal Papilloma In Cage Cultured Mirror Carp (Cyprinus Carpio) In Eğirdir Lake, Öznur Di̇ler, M. Rüştü Özen

Turkish Journal of Veterinary & Animal Sciences

In this study, the histopathology of enzootic epidermal papillomas in cage cultured mirror carp from the Liman region of Eğirdir Lake was investigated. Macroscopically, the lesions were creamy-white, had a villus-like structure and were localised in the operculum, lateral line and fins. Histopathological studies showed that lesions were the result of hyperplasia of fibrous connective tissue and epidermal tissues. The cells of both tissues were faintly stained by HxE and PAS. Under light microscopy, cytoplasmic vacuolation in epidermal cells in the lesions was prominent. Mucous cells were relatively common and larger than normal. Chemotherapy effectively cured all lesions.


The Effects Of An Electromagnetic Field (90 Hz And 5 Mt) On Some Blood Electrolyte (Ca^{++}, P^{+++}, Na^{+}, K^{+}, Cl^{-}) Levels In Male Mice, Gökhan Eraslan, Ali̇ Bi̇lgi̇li̇, Di̇nç Eşsi̇z, Hakan Saltaş Jan 2002

The Effects Of An Electromagnetic Field (90 Hz And 5 Mt) On Some Blood Electrolyte (Ca^{++}, P^{+++}, Na^{+}, K^{+}, Cl^{-}) Levels In Male Mice, Gökhan Eraslan, Ali̇ Bi̇lgi̇li̇, Di̇nç Eşsi̇z, Hakan Saltaş

Turkish Journal of Veterinary & Animal Sciences

The effects of 90 Hz and 5 mT EMF on blood Ca^{++}, P^{+++}, Na^{+}, K^{+} and Cl^{-} levels were investigated. For this purpose, 72 Swiss-Albino mice were used. On days 15, 30 and 45, blood was taken from both the experimental and control groups. A statistically significant decrease in the calcium level was only observed on day 30. On days 15 and 45, a statistically significant increase in the phosphorus level was determined. A significant decrease in the level of plasma potassium and chloride levels and a significant increase in sodium levels were seen throughout the experiment (days 15, 30 …


The Effects Of An Electromagnetic Field (60-90 Hz And 5 Mt) On Blood Electrolyte Levels In Diurnal Rhythm, Gökhan Eraslan, Mehmet Akdoğan, Ali̇ Bi̇lgi̇li̇, Murat Kanbur, Fatma Şahi̇ndokuyucu Jan 2002

The Effects Of An Electromagnetic Field (60-90 Hz And 5 Mt) On Blood Electrolyte Levels In Diurnal Rhythm, Gökhan Eraslan, Mehmet Akdoğan, Ali̇ Bi̇lgi̇li̇, Murat Kanbur, Fatma Şahi̇ndokuyucu

Turkish Journal of Veterinary & Animal Sciences

In this study, 120 British male mice, weighing 35-40 g, and 5 months old were used. Three groups, one control (Group I) and two experimental (Groups II and III), were formed. One experimental group (Group II) received 60 Hz and 5 mT, while the other (Group III) received 90 Hz frequency and 5 mT magnetic flux density efficacy electromagnetic field (EMF), between 900 and 1700. During this period, blood was taken every hour and blood electrolyte levels (Ca^{++}, P^{+++}, Na^{+}, K^{+}, Cl^{-}) were evaluated. In conclusion, in almost all periods and groups only sodium and calcium levels showed a steady …


Investigation Of The Live Weight, Body Measurements And Fiber Diameter Of The Deutsches Merinofleischschaf And Karacabey Merino Sheep Using Cluster Analysis, İsmai̇l Safa Gürcan, Hali̇l Akçapinar Jan 2002

Investigation Of The Live Weight, Body Measurements And Fiber Diameter Of The Deutsches Merinofleischschaf And Karacabey Merino Sheep Using Cluster Analysis, İsmai̇l Safa Gürcan, Hali̇l Akçapinar

Turkish Journal of Veterinary & Animal Sciences

As regards fiber diameter quality and yields, Turkish Merinos have proven successful. They have better fertility and meat yields than native species bred in Turkey. It is of significance to know the similarity degree between Turkish Merinos and Deutsches Merinofleischschaf. Various methods of statistical analysis may be employed to determine the similarity degree between related species. One of the methods to be employed is cluster analysis. In this study, the live weight, body measurements and fiber diameter of these two genotypes were determined by cluster analysis. The hierarchical clustering and SLINK techniques and Euclidean distance method were used for clustering. …


Prevalence And Contamination Levels Of Listeria Spp. In Poultry Minced, Poultry Meatballs And Poultry Burgers, Ufuk Tansel Şi̇reli̇, İrfan Erol, Seyda Şahi̇n, Göknur Terzi̇, O. Alp Gürbüz Jan 2002

Prevalence And Contamination Levels Of Listeria Spp. In Poultry Minced, Poultry Meatballs And Poultry Burgers, Ufuk Tansel Şi̇reli̇, İrfan Erol, Seyda Şahi̇n, Göknur Terzi̇, O. Alp Gürbüz

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to determine the presence and contamination levels of Listeria spp. in 100 samples of chicken meat, including 40 mince meat samples, 30 meatball samples and 30 burger samples which were retailed fresh in the markets of Ankara. USDA/FSIS suggested methods were used for the isolation and identification of Listeria spp., while the number of Listeria spp. was detected by the Most Probable Number (MPN) technique. As a result, it was found that chicken mince samples were contaminated by various Listeria spp. in 85% (34/40) of chicken mince meat samples, 83.3% (25/30) of chicken meatball samples and …


An Experimental Study On The Effect Of Acidic Drinks On Gingival Health In Dogs, Recep Orbak, Ertunç Dayi, Mustafa Atasever Jan 2002

An Experimental Study On The Effect Of Acidic Drinks On Gingival Health In Dogs, Recep Orbak, Ertunç Dayi, Mustafa Atasever

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to conduct a preliminary investigation on the effects of cola drink consumption on gingival health in dogs and thereafter to come up with an opinion as to what extent cola consumption has an impact on gingival health in humans. The study was carried out on 20 healthy dogs (range 2.3-12 years; mean 8.3 years). The dogs were randomly divided into two equal groups. The control group was not given anything containing acid, while the experimental group was given classic Coca Cola® (pH: 2.4) (500 ml daily). All the dogs were fed a number of …


Light Microscopic Investigations On The Oviduct In Hens, Asuman Özen Jan 2002

Light Microscopic Investigations On The Oviduct In Hens, Asuman Özen

Turkish Journal of Veterinary & Animal Sciences

In this study, the oviducts of 10 laying hens aged 8 months and 10 oviducts of pullets aged 2 months were comparatively examined. The oviducts of hens consisted of five regions: infundibulum, magnum, isthmus, uterus and vagina. Tissue samples were taken from the five oviduct regions. The samples were fixed in 10% neutral formalin and embedded in paraffin and then cut into 6 micron sections. Tissue sections were stained with triple, PAS, PAS/Ab, Ab, performic acid/Ab and examined with a light microscope. The epithelium of the oviduct consisted of ciliated and secretory cells. In laying hens, activated tubular glands were …


The Use Of Mini-Titanium Plates For Osteosynthesis In Extremity Fractures Located Near Joints In Cats And Dogs, Hasan Bi̇lgi̇li̇ Jan 2002

The Use Of Mini-Titanium Plates For Osteosynthesis In Extremity Fractures Located Near Joints In Cats And Dogs, Hasan Bi̇lgi̇li̇

Turkish Journal of Veterinary & Animal Sciences

This study was carried out with a total of 23 animals: two dogs and 21 cats. Appropriate mini-titanium plates and screws were selected with the aid of radiographs the day before the operation. All the animals were anesthesized with xylazine hydrochloride 2.3 mg/kg IM (Rompun, Bayer, Turkey) premedication followed by ketamine hydrochloride 10 mg/kg IM (Ketalar, Eczacıbaşı, Turkey) injection. The selected mini-titanium plates were shaped according to the fractured bone and applied. All the animals were monitored 1, 3, 5, 7 and 9 weeks postoperatively by means of clinical and radiological examinations. Twenty-one animals showed no postoperative complications; all the …


The Effects Of Gonadotrophins On In Vitro Maturation Of Cat Oocytes, Mi̇that Evecen, Alper Baran, Çağatay Tek Jan 2002

The Effects Of Gonadotrophins On In Vitro Maturation Of Cat Oocytes, Mi̇that Evecen, Alper Baran, Çağatay Tek

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the effects of FSH and LH hormones on in vitro development of cat oocytes. The ovaries from spayed cats were brought to the laboratory in +4 °C PBS solution two hours after being collected. The oocytes were recovered by slicing of ovaries and were separated into two groups: Group 1 (Control); TCM 199 + 5% FCS + 2.2 g/l NaHCO_3 + 0.23 mM Na Pyruvate; and Group 2 (Treatment); TCM 199 + 5% FCS + 2.2 g/l NaHCO_3 + 0.23mM Na Pyruvate + FSH (Sigma F-2293) (1 µg/ml) + LH (Sigma L-5269) …


A Study On Growth, Fattening Performance And Carcass Characteristics Of Morkaraman And Kıvırcık X Morkaraman Crosses (B_1) Lambs, Mürsel Küçük, Davut Bayram, Orhan Yilmaz Jan 2002

A Study On Growth, Fattening Performance And Carcass Characteristics Of Morkaraman And Kıvırcık X Morkaraman Crosses (B_1) Lambs, Mürsel Küçük, Davut Bayram, Orhan Yilmaz

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to compare the growth, fattening performance and carcass characteristics of Morkaraman (M) and Kıvırcık (K) x (K x M) Bı lambs. Fifty-four M and 57 K x (K x M) Bı male and female lambs were used for growth traits and 18 M and 30 K x (K x M) Bı for fattening traits, and 6 male lambs for slaughter and carcass traits. Growth was investigated from the birth to weaning (day 90). The lambs were fed, after weaning at approximately 25 kg live weight, with sainfoin and concentrates ad libitum. Fattening lasted 56 days. …


The Effect Of Aflatoxin B1 On The Vitamin A Storage In The Livers Of Broiler Chicks, Berri̇n Salmanoğlu Jan 2002

The Effect Of Aflatoxin B1 On The Vitamin A Storage In The Livers Of Broiler Chicks, Berri̇n Salmanoğlu

Turkish Journal of Veterinary & Animal Sciences

The effect of AFB1 in feed on the liver and serum vitamin A and b-carotene levels and the ability of the liver to stor vitamin A in broiler chicks were investigated. Twenty day-old Ross broiler chicks were randomly allocated into 4 groups with 5 each in the control group, group I, group II, and group III. The chicks of the control group and group I were fed ad libitum on broiler chick growing feed. The chicks of group II and group III were fed ad libitum on feed with AFB1 (4 ppm/kg) feed. On the seventh day, vitamin A palmitate …


Use Of Milk Progesterone Assay And Rectal Palpation To Monitor Postpartum Anoestrus And The Effect Of Prid-Pmsg Treatment In Murrah Buffaloes, Mehmet Uçar, Mustafa Küçükkebapçi, Muzaffer Çelebi̇, Havva Neşe Akalin Jan 2002

Use Of Milk Progesterone Assay And Rectal Palpation To Monitor Postpartum Anoestrus And The Effect Of Prid-Pmsg Treatment In Murrah Buffaloes, Mehmet Uçar, Mustafa Küçükkebapçi, Muzaffer Çelebi̇, Havva Neşe Akalin

Turkish Journal of Veterinary & Animal Sciences

Postpartum anoestrus is widely known to cause infertility in buffaloes. This study was conducted to determine the problem of postpartum anoestrus using rectal palpation and milk progesterone level in 8 Murrah buffaloes calved during late spring or early summer in the Kocatepe Agricultural Research Institute, Afyon, Turkey. In addition, the pregnancy rates of experimental animals were investigated using a combined therapy of a progesterone releasing intravaginal device (PRID) and pregnant mare serum gonadotropin (PMSG). The ovaries of each buffalo were examined by rectal palpation once a week and the milk progesterone level was detected twice a week using enzyme immunoassay …


Effects Of Selenium On Thyroid Hormone Levels In Sheep, Ahmet Ateşşahi̇n, İbrahi̇m Pi̇ri̇nçci̇, Feri̇t Gürsu, Gürkan Çikim Jan 2002

Effects Of Selenium On Thyroid Hormone Levels In Sheep, Ahmet Ateşşahi̇n, İbrahi̇m Pi̇ri̇nçci̇, Feri̇t Gürsu, Gürkan Çikim

Turkish Journal of Veterinary & Animal Sciences

In this study, the thyroid hormone levels in sheep experimentally treated with selenium were investigated. Twelve Akkaraman sheep were used in the investigation. Selenium was given intramuscularly at doses of 0.1 (Group 1) and 0.4 mg/kg (Group 2). The blood samples were taken from the jugular vein at 1, 2, 4, 8, 24, 48, 96 and 144 hours after the administration. Serum triiodotyronine (T3), thyroxin (T4), free triiodotironine (fT3) and free thyroxin (fT4) levels were determined using a hormone autoanalyzer. In the comparison between the groups, it was shown that serum T3 levels reached the maximum levels at the 96^{th} …


Transfer Of In Vitro Produced Sheep Embryos, Sema Bi̇rler, Serhat Pabuccuoğlu, Hatem Atalla, Serhat Alkan, Özen Banu Özdaş, Süleyman Bacinoğlu, Ümüt Ci̇ri̇t, İrem Zavar, Mehmet Emi̇n Cumhur Sönmez, İrfan Kamuran İleri̇ Jan 2002

Transfer Of In Vitro Produced Sheep Embryos, Sema Bi̇rler, Serhat Pabuccuoğlu, Hatem Atalla, Serhat Alkan, Özen Banu Özdaş, Süleyman Bacinoğlu, Ümüt Ci̇ri̇t, İrem Zavar, Mehmet Emi̇n Cumhur Sönmez, İrfan Kamuran İleri̇

Turkish Journal of Veterinary & Animal Sciences

The objective of the present study was to transfer sheep embryos produced in vitro to recipient ewes. Ovaries were taken from slaughtered Kıvırcık ewes and transferred to the laboratory in phosphate buffered saline (PBS) at 30-35 ºC. The cumulus-oocyte complexes were obtained by slicing and washing 1-6 mm diameter follicles and matured for 24 h in medium 199 supplemented with sodium pyruvate, follicle stimulating hormone (FSH), luteinizing hormone (LH) and 10% fetal calf serum (FCS) at 38.5 ºC under 5% CO_2 in humidified atmosphere. Fresh semen was collected from three Kıvırcık rams, pooled and prepared for in vitro fertilization by …


Evaluation Of The Periventricular Lesions Associated With Congenital Hydrocephalus In Puppies, Osman Kutsal, Rifki Haziroğlu, Tolga Güvenç, Recai̇ Tunca Jan 2002

Evaluation Of The Periventricular Lesions Associated With Congenital Hydrocephalus In Puppies, Osman Kutsal, Rifki Haziroğlu, Tolga Güvenç, Recai̇ Tunca

Turkish Journal of Veterinary & Animal Sciences

Hydrocephalus associated lesions such as diverticula, clefts and tears observed in periventricular white matter were studied pathologically in three puppies with necropsy between 1993 and 1994. Enlargement of the cranial vault was present in the puppies. The animals were blind, and their morphological alterations were similar. Marked hydrocephalus caused by mesencephalic canal malformations and obstruction was detected in all cases. Compression atrophy was seen in the nucleus caudatus, hippocampus, putamen and cerebellum in all animals. Cerebral sulci malformations were also detected in the cranial parts of the brain. Ependymal lesions consisting of attenuation, regeneration, calcification and desquamation were also observed. …


Identification Of Chicken Originated Campylobacter Coli And Campylobacter Jejuni By Polymerase Chain Reaction (Pcr), Hasan Basri̇ Ertaş, Burhan Çeti̇nkaya, Adi̇le Muz, Hasan Öngör Jan 2002

Identification Of Chicken Originated Campylobacter Coli And Campylobacter Jejuni By Polymerase Chain Reaction (Pcr), Hasan Basri̇ Ertaş, Burhan Çeti̇nkaya, Adi̇le Muz, Hasan Öngör

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to isolate Campylobacter species from the intestines and livers of chicken and to identify Campylobacter coli and Campylobacter jejuni by both conventional methods and Polymerase Chain Reaction (PCR). Four specific primers derived from the ceuE gene present in the genomes of C. coli and C. jejuni were used for PCR identification. In the examination of 150 intestine and liver samples by culture and PCR, 25 (16.6%) and 32 (21.3%) were identified as C. coli and C. jejuni, respectively. It was concluded that the PCR assay used in this study may successfully be applied for …


The Effects Of Bsa And Fcs On In Vitro Maturation Of Bitch Oocytes, Mi̇that Evecen, Alper Baran, Serhat Alkan, Ö. Banu Özdaş, Çağatay Tek Jan 2002

The Effects Of Bsa And Fcs On In Vitro Maturation Of Bitch Oocytes, Mi̇that Evecen, Alper Baran, Serhat Alkan, Ö. Banu Özdaş, Çağatay Tek

Turkish Journal of Veterinary & Animal Sciences

The effects of protein sources added to the medium for maturation of bitch oocytes were investigated. Ovaries from spayed bitches were transported to the laboratory in PBS at 38 ºC within an hour. Recovered oocytes were divided into three groups and left for 72 h maturation at 38.5 ºC under an atmosphere of 5% CO_2 and high humidity. Group 1 (Control): TCM 199 medium, Group 2 (BSA): TCM 199 medium supplemented with 0.3% BSA, Group 3 (FCS): TCM 199 medium supplemented with 5% FCS. At the end of the maturation period, all oocytes were denuded from cumulus cells by pipetting …


Ultrasonographic Evaluation Of Liver Damage And Obstruction Of The Biliary Tract In Experimentally Bile Duct Ligated Dogs, Remzi̇ Gönül, Yücel Meral, Abdullah Kayar, Utku Bakirel, Mehmet Erman Or, Gülbi̇n Şennazli, Aydin Gürel, Zi̇ya Aslan Jan 2002

Ultrasonographic Evaluation Of Liver Damage And Obstruction Of The Biliary Tract In Experimentally Bile Duct Ligated Dogs, Remzi̇ Gönül, Yücel Meral, Abdullah Kayar, Utku Bakirel, Mehmet Erman Or, Gülbi̇n Şennazli, Aydin Gürel, Zi̇ya Aslan

Turkish Journal of Veterinary & Animal Sciences

The liver damage and obstructions of the biliary tract were evaluated ultrasonographically in experimentally bile duct ligated dogs. Seven healthy dogs of different breeds were used. The common bile duct was double ligated at the duodenal region after clinical and ultrasonographic examinations. After that, clinical examinations, biochemical analyses and examinations of the liver, gallbladder and biliary tract were done ultrasonographically over 6 weeks. All dogs were necropsied and macroscopic and histologic alterations were determined at the end of the study. It was determined that the levels of serum ALT, ALP, GGT and T. Bilirubin were (p < 0.001-0.05) statistically significantly increased after the operation in all dogs. As a result of ultrasonographic examinations, it was determined that the bile ducts and gallbladder were distended, the neck of the gallbladder was larger and more tortuous than normal, hypoechoic sludge was prominent in the gallbladder, and perivascular, multifocal hyperechoic areas appeared in the liver parenchyma toward the end of the study. These alterations were confirmed macroscopically and microscopically at the end of the autopsy in all dogs. We consider that as well as blood serum parameters, ultrasonographic examinations are useful in the early diagnosis of biliary diseases and in differentiating them from hepatic diseases.


Efficacy Of A Pneumatic Oxygen Conserving Device In The Oxygenation Of Copd Patients During Sleep, Ronda Z. Bradley Jan 2002

Efficacy Of A Pneumatic Oxygen Conserving Device In The Oxygenation Of Copd Patients During Sleep, Ronda Z. Bradley

Theses

For the greater than one million Americans who rely on supplemental oxygen for survival, the vast majority of them struggle with ambulatory oxygen sources which restrict their ability to move freely about within and out of their homes. Liquid oxygen sources can provide the smallest and longest lasting ambulatory oxygen supply. However, the cost of the provision on liquid oxygen has historically been cost prohibitive under our current Medicare reimbursement schedule. The HELiOS™ Oxygen System by Puritan-Bennett a division of Mallinckrodt. has developed an oxygen system, which allows for the smallest, lightest weight and longest lasting oxygen system ever developed. …


Evaluating The Prevalence, Content And Readability Of Complementary And Alternative Medicine (Cam) Web Pages On The Internet, Smitha Sagaram, Muhammad Walji, Elmer Bernstam Jan 2002

Evaluating The Prevalence, Content And Readability Of Complementary And Alternative Medicine (Cam) Web Pages On The Internet, Smitha Sagaram, Muhammad Walji, Elmer Bernstam

Faculty, Staff and Student Publications

Complementary and alternative medicine (CAM) use is growing rapidly. As CAM is relatively unregulated, it is important to evaluate the type and availability of CAM information. The goal of this study is to deter-mine the prevalence, content and readability of online CAM information based on searches for arthritis, diabetes and fibromyalgia using four common search engines. Fifty-eight of 599 web pages retrieved by a "condition search" (9.6%) were CAM-oriented. Of 216 CAM pages found by the "condition" and "condition + herbs" searches, 78% were authored by commercial organizations, whose pur-pose involved commerce 69% of the time and 52.3% had no …


Evaluating A Medical Error Taxonomy, Juliana Brixey, Todd R. Johnson, Jiajie Zhang Jan 2002

Evaluating A Medical Error Taxonomy, Juliana Brixey, Todd R. Johnson, Jiajie Zhang

Faculty, Staff and Student Publications

Healthcare has been slow in using human factors principles to reduce medical errors. The Center for Devices and Radiological Health (CDRH) recognizes that a lack of attention to human factors during product development may lead to errors that have the potential for patient injury, or even death. In response to the need for reducing medication errors, the National Coordinating Council for Medication Errors Reporting and Prevention (NCC MERP) released the NCC MERP taxonomy that provides a standard language for reporting medication errors. This project maps the NCC MERP taxonomy of medication error to MedWatch medical errors involving infusion pumps. Of …


Antisocial Alcoholism And Serotonin-Related Polymorphisms: Association Tests, Elizabeth M. Hill, Scott F. Stoltenberg, Katherine Harris Bullard, Sheng Li, Robert A. Zucker, Marget Burmeister Jan 2002

Antisocial Alcoholism And Serotonin-Related Polymorphisms: Association Tests, Elizabeth M. Hill, Scott F. Stoltenberg, Katherine Harris Bullard, Sheng Li, Robert A. Zucker, Marget Burmeister

Department of Psychology: Faculty Publications

Central serotonin dysfunction appears to be related to a subtype of alcoholism with antisocial impulsive features (type II; antisocial alcoholism). The serotonergic deficit may be associated with greater impulsivity, which in turn facilitates both alcohol dependence and antisocial behavior. The present study tested association of antisocial impulsive alcoholism with candidate genes related to serotonergic neurotransmission, using families. Eight markers were assayed using polymerase chain reaction: tryptophan hydroxylase (intron 7), the serotonin transporter SLC6A4 (VNTR 9/12), HTTLPR, the three serotonin receptor types HTR1B (G861C), HTR2A (T102C) and HTR2C (Cys23Ser), monoamine oxidase A (T1460C), and (CA)n. Eligible probands had early …


Small Glutamine-Rich Protein/Viral Protein U–Binding Protein Is A Novel Cochaperone That Affects Heat Shock Protein 70 Activity, Peter C. Angeletti, Doriann Walker, Antonito T. Panganiban Jan 2002

Small Glutamine-Rich Protein/Viral Protein U–Binding Protein Is A Novel Cochaperone That Affects Heat Shock Protein 70 Activity, Peter C. Angeletti, Doriann Walker, Antonito T. Panganiban

Nebraska Center for Virology: Faculty Publications

Molecular chaperone complexes containing heat shock protein (Hsp) 70 and Hsp90 are regulated by cochaperones, including a subclass of regulators, such as Hsp70 interacting protein (Hip), C-terminus of Hsp70 interacting protein (CHIP), and Hsp70-Hsp90 organizing factor (Hop), that contain tetratricopeptide repeats (TPRs), where Hsp70 refers to Hsp70 and its nearly identical constitutive counterpart, Hsc70, together. These proteins interact with the Hsp70 to regulate adenosine triphosphatase (ATPase) and folding activities or to generate the chaperone complex. Here we provide evidence that small glutamine-rich protein/viral protein U–binding protein (SGT/UBP) is a cochaperone that negatively regulates Hsp70. By “Far-Western” and pull-down assays, SGT/UBP …


Methods And Compositions For Protection Against Bovine Viral Diseases, Subramaniam Srikumaran Jan 2002

Methods And Compositions For Protection Against Bovine Viral Diseases, Subramaniam Srikumaran

School of Veterinary and Biomedical Sciences: Faculty Publications

The present invention relates to methods and compositions for eliciting an immune response against bovine viral epitopes. The methods comprise combining at least one heat shock protein with at least one bovine viral epitope to form a purified epitope/heat shock protein complex and administration of an immune system stimulating amount of the purified epitope/heat shock protein complex. The compositions comprise, a purified epitope/heat shock protein complex comprising at least one bovine viral epitope complexed with at least one heat shock protein, and a pharmaceutically acceptable carrier, diluent or excipient.


Mycobacterium Smegmatis D-Alanine Racemase Mutants Are Not Dependent On D-Alanine For Growth, Ofelia Chacon, Zhengyu Feng, N. Beth Harris , Nancy E. Caceres, Garry Adams, Raul G. Barletta Jan 2002

Mycobacterium Smegmatis D-Alanine Racemase Mutants Are Not Dependent On D-Alanine For Growth, Ofelia Chacon, Zhengyu Feng, N. Beth Harris , Nancy E. Caceres, Garry Adams, Raul G. Barletta

School of Veterinary and Biomedical Sciences: Faculty Publications

Mycobacterium smegmatis is a fast-growing nonpathogenic species particularly useful in studying basic cellular processes of relevance to pathogenic mycobacteria. This study focused on the D-alanine racemase gene (alrA), which is involved in the synthesis of D-alanine, a basic component of peptidoglycan that forms the backbone of the cell wall. M. smegmatis alrA null mutants were generated by homologous recombination using a kanamycin resistance marker for insertional inactivation. Mutants were selected on Middlebrook medium supplemented with 50 mM D-alanine and 20 μg of kanamycin per ml. These mutants were also able to grow in standard and minimal media without …


Detection Of Giardia Duodenalis Antigen In Coprolites Using A Commercially Available Enzyme-Linked Immunosorbent Assay, Marcelo Luiz Carvalho Gonçalves, Adauto Araújo, Rosemere Duarte, Joaquim Pereira Da Silva, Karl Reinhard, Françoise Bouchet, Luis Fernando Ferreira Jan 2002

Detection Of Giardia Duodenalis Antigen In Coprolites Using A Commercially Available Enzyme-Linked Immunosorbent Assay, Marcelo Luiz Carvalho Gonçalves, Adauto Araújo, Rosemere Duarte, Joaquim Pereira Da Silva, Karl Reinhard, Françoise Bouchet, Luis Fernando Ferreira

Karl Reinhard Publications

The objective of this experiment was to assess the utility of a commercially available enzyme-linked immunosorbent assay (ELISA) kit for diagnosis of giardiasis in archaeological human remains. The kit, a monoclonal antibody assay, is used to detect the presence of Giardia-specific antigen 65 (GSA65) in human feces. We utilized the assay in ancient fecal material. The material included desiccated feces found in mummies or in archaeological sites, and sediments from latrines. A total of 83 specimens, previously examined microscopically for parasites, were examined. The ELISA detected 3 positive samples, dated to about 1200 AD, 1600 AD, and 1700 AD. …


Multidisciplinary Coprolite Analysis, Karl Reinhard, Dennis R. Danielson, Mark Daniels, Sérgio Augusto De Miranda Chaves Jan 2002

Multidisciplinary Coprolite Analysis, Karl Reinhard, Dennis R. Danielson, Mark Daniels, Sérgio Augusto De Miranda Chaves

Karl Reinhard Publications

Coprolite analysis, as reviewed by Reinhard and Bryant (1992), contributes unique and detailed information regarding diet and parasitic disease. We present here an analysis of dietary components of coprolites from Bighorn Cave using macroscopic remains, pollen concentrations, and phytoliths. In addition, we analyzed Bighorn Cave coprolites for evidence of parasitic organisms, especially intestinal worms. Such analyses of coprolites have become important methods for reconstructing past dietary and medicinal practices. Pollen concentration and phytolith quantification techniques have recently been developed, but until this report no known attempt has been made to synthesize pollen, macroscopic, and phytolith data from a single coprolite …


Introduction To Written Symposium On Public Health And International Law, David P. Fidler Jan 2002

Introduction To Written Symposium On Public Health And International Law, David P. Fidler

Articles by Maurer Faculty

No abstract provided.