Medicine and Health Sciences Commons^™

Anti-Class Ii Monoclonal Antibody-Targeted Vibrio Cholerae Tcpa Pilin: Modulation Of Serologic Response, Epitope Specificity, And Isotype, Jia-Yan Wu, Ronald K. Taylor, William F. Wade

Dartmouth Scholarship

Toxin-coregulated pilus (TCP) is a colonization factor required for cholera infection. It is not a strong immunogen when delivered in the context of whole cells, yet pilus subunits or TcpA derivative synthetic peptides induce protective responses. We examined the efficacy of immunizing mice with TCP conjugated to anti-class II monoclonal antibodies (MAb) with or without the addition of cholera toxin (CT) or anti-CD40 MAb to determine if the serologic response to TcpA could be manipulated. Anti-class II MAb-targeted TCP influenced the anti-TCP peptide serologic response with respect to titer and isotype. Responses to TcpA peptide 4 were induced with class …

Evaluation Of A Tetracycline-Inducible Promoter In Staphylococcus Aureus In Vitro And In Vivo And Its Application In Demonstrating The Role Of Sigb In Microcolony Formation, B. T. Bateman, N. P. Donegan, T. M. Jarry, M. Palma

Dartmouth Scholarship

An inducible promoter system provides a powerful tool for studying the genetic basis for virulence. A variety of inducible systems have been used in other organisms, including pXyl-xylR-inducible promoter, the pSpac-lacI system, and the arabinose-inducible P_BAD promoter, but each of these systems has limitations in its application to Staphylococcus aureus. In this study, we demonstrated the efficacy of a tetracycline-inducible promoter system in inducing gene expression in S. aureus in vitro and inside epithelial cells as well as in an animal model of infection. Using the xyl/tetO promoter::gfp_uvr fusion carried on a shuttle …

Evaluation Of Cholera Vaccines Formulated With Toxin-Coregulated Pilin Peptide Plus Polymer Adjuvant In Mice, Jia-Yan Wu, William F. Wade, Ronald K. Taylor

Dartmouth Scholarship

Cholera is an acute diarrheal disease that is caused by the gram-negative bacterium Vibrio cholerae. The low efficacy of currently available killed-whole-cell vaccines and the reactinogenicity coupled with potential reversion of live vaccines have thus far precluded widespread vaccination for the control of cholera. Recent studies on the molecular nature of the virulence components that contribute to V. cholerae pathogenesis have provided insights into possible approaches for the development of a defined subunit cholera vaccine. Genetic analysis has demonstrated that the toxin-coregulated pilus (TCP) is the major factor that contributes to colonization of the human intestine by V. cholerae. In …

Immune Response Genes Modulate Serologic Responses To Vibrio Cholerae Tcpa Pilin Peptides, Michael D. Meeks, Terri K. Wade, Ronald K. Taylor, William F. Wade

Dartmouth Scholarship

Cholera is an enteric disease caused by Vibrio cholerae. Toxin-coregulated pilus (TCP), a type 4 pilus expressed by V. cholerae, is a cholera virulence factor that is required for host colonization. The TCP polymer is composed of subunits of TcpA pilin. Antibodies directed against TcpA are protective in animal models of cholera. While natural or recombinant forms of TcpA are difficult to purify to homogeneity, it is anticipated that synthesized TcpA peptides might serve as immunogens in a subunit vaccine. We wanted to assess the potential for effects of the immune response (Ir) gene that could complicate a peptide-based …

Access To Pediatric Services Under Medicaid Managed Care In The District Of Columbia, Karen Shaw, Sara J. Rosenbaum

Health Policy and Management Faculty Publications

No abstract provided.

Metastatic Prostate Cancer Associated With Lumbar Compression Fracture And Spinal Stenosis: A Casereport And Literature Review, Shwu-Fen Sun, Jue-Long Wang, Ping-Hong Lai

Rehabilitation Practice and Science

We reported a 72 y/o male who had past history of old L1 compression fracture for 1 year. He suffered from falling down accidentally and was operated under impression of L1 burst fracture with cauda equina syndrome. He could ambulate with walker, but persistent back pain was still complained. Two months after operation, he became paralyzed rapidly in 2 days. Neurological examinations showed decreased motor strength of bilateral lower extremities to grade 0-1/5, sensory deficit below T10 dermatome and bilateral extensor plantar response. X-ray film showed an osteoblastic lesion in T9 vertebra body. Magnetic resonance Imaging (MRI) of thoracic spine …

The Interaction Between Antidepressants And Spasticity Of Stroke: Casereport, Shih-Wen Lin, Zong-Jing Wang, Young-Hue Yu

Rehabilitation Practice and Science

The upper motor neuron diseases (such as cerebrovascular accidents, traumatic brain injury, spinal cord injury, and multiple sclerosis) frequently cause spasticity of extremities. The medications for treating spasticity include baclofen and diazepam, which take their effect in the central nervous system through their GABAergic action. The incidence of depression in patients with spasticity is much higher than that of the normal population. Many doctors prescribe antidepressants for these patients. Two common used antidepressants are tricyclic antidepressants (TCA) and selective serotonin reuptake inhibitors (SSRI). They could increase serotonin amount in synapses, and induce increased excitability of motor neurons, which in turn …

A Design Of Rehabilitation Information System For Inpatients: An Experience Of A Medical Center, Mu-Jung Kao, Chih-Tsung Hsieh, Rong-Lin Yang, Mei-Wun Tsai

Rehabilitation Practice and Science

Rehabilitation Information System for Inpatients (RISI) was designed for medical administration in the Department of Rehabilitation at a medical center in Taiwan. The RISI includes administration system, patient consultation system, rehabilitation order entry system, rehabilitation therapy system for physical therapy, occupational therapy, and speech therapy, check out system for time and frequency of therapy, and statistic function system.The characteristics of this system includes: 1) integration of systems between outpatients and inpatients; 2) integration of available administration system and patient consultation system; 3) integration of administration and medical system in the department of rehabilitation and 4) operation without paper work. The …

Clinical Experience In Asian Type Multiple Sclerosis, Der-Sheng Han, Jau-Jia Lin, Yen-Ho Wang, Chih-Chao Yang

Rehabilitation Practice and Science

Multiple sclerosis is a heterogeneous disease entity, and also the most common disabling neurological disorder of people in middle life in USA. The prevalence of multiple sclerosis in Asia is lower than that in Western countries of similar latitude. Besides, there are some different features, clinically as well as pathologically, between this disease in Asia and in Western countries. We collected 27 cases diagnosed as clinically definite MS at NTUH between 1990-2000, whose clinical presentations are restricted to optic nerves and spinal cord. Their clinical charts were reviewed retrospectively, and the data was collected in a standard coding sheet. The …

Genital Tract Lesions And The Isolation Of Ureaplasma Diversum From The Reproductive Tract Of Normal And Repeat Breeding Dairy Cows, Prachin Virakul, Kraiwan Hongyantarachai, Kitti Mahaviroon, Saroch Ngarmkum, Phornchai Suwannaphirom, Vinai Krasaesinthugomol, Ayuth Harintharanont, Santi Prasithphol, Pairoj Ampawanwong, Junpen Suwimonteerabutr

The Thai Journal of Veterinary Medicine

This study was conducted in 78 dairy cows(50 repeat breeders and 28 normal cows, with no history of breeding difficulty) from 6 livestock regions of Thailand. Observations were made on the lesions of granular vulvitis and bacteriology performed in order to isolate Ureaplasma diversum. Swabs were taken from 3 sites; the clitoral fossa, the dorsal vulva lips and the external cervical osteum. The isolation of U. diversum was carried out using Taylor Robinson Medium. In the repeat breeding group, U. diversum was isolated from 46% of the clitoral swabs, 48% of the vulva lip swabs and 22% of the external …

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

What Is Your Diagnosis?, Pranee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.

A Decentralized, Patient-Centered Approach To Diabetes Disease Management In The Primary Care Setting, Michael Toscani, John P. O'Connor, David B. Nash

College of Population Health Faculty Papers

Although many disease management programs have been developed for diabetes, no single design has proved best for all providers and patient populations. Cost effectiveness is especially relevant to diabetes programs because significant costs of the disease may come from complications that occur later in life, while the costs of the program are incurred immediately. For this reason, diabetes disease management programs with positive outcomes and low implementation costs are of particular importance. We report here on the outcomes of a pilot test of the Steps to Health program developed by Abbott Laboratories. The Steps to Health program was designed to …

From Product Dispensing To Patient Care: The Role Of The Pharmacist In Providing Pharmaceutical Care As Part Of An Integrated Disease Management Approach, Laura T. Pizzi, Jean M. Menz, Geneen R. Graber, Dong-Churl Suh

College of Population Health Faculty Papers

During the past decade, the profession of pharmacy has changed dramatically. The Doctor of Pharmacy degree has replaced the Bachelor of Science degree as the first professional degree offered at most accredited U.S. pharmacy schools. Advanced clinical training is now a mainstay of pharmacy training, and this has enabled pharmacists to contribute to disease management efforts. In addition, technological improvements in prescription processing have afforded pharmacists more time to participate in disease management activities. This paper describes how the role of the pharmacist has changed and reviews the results of programs involving pharmacists as disease management providers in the areas …

Physical Activity Research In Nursing, Lorraine B. Robbins, Nola J. Pender, Vicki S. Conn, Marilyn Frenn, Geri B. Neuberger, Mary A. Nies, Robert V. Topp, Joellen Wilbur

College of Nursing Faculty Research and Publications

Purpose: To present exemplars of physical activity research in nursing, illustrate the importance of physical activity research across the lifespan, and recommend directions for theory development and research.

Methods: Studies of physical activity and exercise currently being conducted by nurse investigators were reported and critiqued by attendees of the Midwest Nursing Research Society 2000 Preconference session entitled “Promoting Physical Activity Among Diverse Groups Across the Health Continuum.” Physical activity and exercise literature during the past decade was reviewed. Databases searched included Medline, CINAHL, Wilson, and ERIC.

Findings: Investigators have emphasized the need to evaluate the effects of theory-based physical activity …

Bacteremia Associated With Early Childhood Caries, Eddie Chang

Loma Linda University Electronic Theses, Dissertations & Projects

Previous research has investigated the post-operative bacteria levels caused by a variety of clinical procedures such as anesthetic injections, standard dental procedures, endodontic treatment, periodontal surgery, root scaling, and oral surgical procedures in children. Other studies have suggested that bacteremias are often caused by oral infections. Although numerous studies have examined how oral infections lead to various medical conditions (i.e. brain abscess) none have studied the potential for preoperative bacteremia that may arise in conjunction with early childhood caries (ECC). The purpose of this study was to compare potential systemic facultative and anaerobic bacteria found in otherwise healthy children who …

Rumination And Cigarette Smoking: A Bad Combination For Depressive Outcomes?, Malia Richmond, Bonnie Spring, Beth Kaplan Sommerfeld, Dennis E. Mcchargue

Department of Psychology: Faculty Publications

Ruminative coping has been shown to heighten the risk and severity of depression. The authors hypothesized that ruminators who smoke would experience greater depressive symptoms than ruminators who do not. The rationale is that, by heightening attentional focus, nicotine may increase ruminators' ability to focus on negative thoughts, augmenting depressed mood. Participants (N = 145) self-reported smoking status, rumination, and current and lifetime depressive symptoms, including depressed mood. Results showed that rumination accounted for a larger amount of variance in current and past depressed mood and severity of lifetime depressive symptoms among smokers than nonsmokers. Noncorrelational, experimental research should …

Characterization Of Seca-Sod Operon In Borrellia Burgdorferi., Tonya Lynn Nichols

Electronic Theses and Dissertations

Borrelia burgdorferi, the causative agent of Lyme disease, has been characterized as a microaerophilic spirochete. O2 consumption and utilization potentially yield reactive oxygen intermediates, such as superoxide, hydroxyl radicals, and hydrogen peroxide. This study investigated the expression of the sod gene, which encodes the only, identified oxidative defense mechanism in B. burgdorferi. Using primer extension analysis and RT-PCR, it was found that sod and secA are organized as a single transcriptional unit under the control of σ70-like promoter upstream of the secA open reading frame. Generally, gene expression decreases with increased distance from the promoter; however, secA expression was observed …

The Pizzi Holistic Wellness Assessment, Michael Pizzi

Occupational Therapy Faculty Publications

This paper describes the Pizzi Holistic Wellness Assessment tool. Using theory from the field of health promotion and expertise gained in his private home health practice, the author developed and pilot tested this assessment on a variety of individuals. The assessment is designed to be used with different populations in a variety of settings to help clients self assess their health and well-being.

Effect Of Monounsaturated Fat Rich Almonds On Hemostatic And Inflammatory Factors In Healthy Adults, Kristianne M. Connell

Loma Linda University Electronic Theses, Dissertations & Projects

Background: The frequent consumption of nuts is inversely associated with the incidence of cardiovascular disease. Nuts are known to decrease the risk of cardiovascular disease by reducing serum total and low-density lipoprotein (LDL) cholesterol. Markers of inflammation including C-reactive protein, E-selectin and interleukin-6 have been proposed to be newer risk factors for cardiovascular disease. However, the influence of nuts such as almonds on markers of inflammation and hemostasis that influence cardiovascular disease risk is not currently known.

Objective: The objective of this study was to determine the effect of almond consumption on markers of inflammation and hemostatic factors in healthy …

Factors Influencing Puberty Attainment In Gilts And Policies That Can Improve Mating Efficiency In Replacement Gilts, Padet Tummaruk, Wichai Tantasuparuk, Mongkol Techakumphu, Annop Kunavongkrit

The Thai Journal of Veterinary Medicine

The objective of this study is to review the information regarding the development of puberty in gilts and how it influences subsequent reproductive performance such an understanding can be used to improve mating efficiency in gilts. The percentage of gilts in a herd strongly influences overall productivity. The first management decision that has to be made about gilts entering the breeding herd, is the timing of the first breeding. This largely depends on when puberty develops. Puberty in gilts is defined as the time when the first oestrus and ovulation occurs followed by regular oestrous cycles. On average, gilts attain …

Immunohistochemical Detection Of Feline Viral Rhinotracheitis In A Cat, Nopadon Pirarat, Yumi Une, Yasuo Nomura

The Thai Journal of Veterinary Medicine

A female, 10-month old, mixed breed cat showed clinical signs of severe dehydration, purulent nasal discharge, dyspnea, severe crepitating lung sounds and no response to antibiotic treatment. Upon necropsy, histopathology revealed severe, diffuse, fibrinopurulent pleuropneumonia and numerous, homogeneous, amphophilic, intranuclear inclusion bodies in the pulmonary alveolar macrophages, bronchial and alveolar epithelium and bronchial glandular epitheliums. Immunohistochemical studies using 3 different monoclonal, anti-Feline Herpesvirus type 1 antibodies, revealed immunoreactivity of viral antigen in the lung that was associated with the histopathological changes. Monoclonal, anti-glycoprotein B, antibody showed the strongest immunoreactivity.

Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn, Sumpun Thammacharoen, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

Effects Of A Serine 364 To Proline Mutation Of The Connexin43 Protein In Transgenic Mice, Chia-Ling Nhan-Chang

Loma Linda University Electronic Theses, Dissertations & Projects

[Abstract Not Included]

Relationship Between Nursing Nutrition Knowledge And Attitudes And Identification Of Malnutrition In The Acute Care Setting, Susan D. Kendziorski

Masters Theses

Malnutrition is widespread in healthcare and effective protocols are needed to identify those at risk. Nurses are well suited to carry out screening for malnutrition because they are in close contact with the patient and are responsible to coordinate each patient's care. In order to perform this role nurses must have nutrition knowledge and the skills to complete an accurate nutrition screen. The purpose of this study was to examine relationships between nursing nutrition knowledge, nursing attitudes towards nutrition, and the accurate completion of a nutrition screening protocol. Patient's charts were reviewed for accurate completion of the nutrition screening protocol …

Etiology And Rehabilitation Effect Of Stroke In Child: A Casereport, Li-Chen Tung, Kwok-Tak Yeung, Chun-Hou Wang, Wen-I Liang, Liu-Ing Bih

Rehabilitation Practice and Science

The incidence of strokes in childhood is 2.5/100,000. Of these, occlusive stroke accounts for 57 to 85 percent, and hemorrhagic stroke accounts for 15 to 51 percent. Occlusive stroke is primarily caused by congenital heart defects, and the principal symptom is acute hemiplegia. Hemorrhagic stroke is caused mostly by abnormalities of blood vessels, and is characterized by headache and loss of consciousness. Compared to adults, children have better collateral circulation and incompletely developed brains. The clinical and recovery courses those in children are different from those of adults. The prognosis and the incidence of stroke recurrence are largely determined by …

Jefferson Alumni Bulletin – Volume 51, Number 1, December 2001

The Bulletin (formerly the Jefferson Medical College Alumni Bulletin)

Jefferson Alumni Bulletin – Volume 51, Number 1, December 2001 Building a dynamic multicultural community at Jefferson, page 4 Farber institute for neurosciences will be headed by Samuel Gandy, page 7 Dean Thomas Nasca’s strategic plan, page 10 Bud Bacharach’56 receives the cornerstone award, page 18

Polycyclic Aromatic Hydrocarbon Degradation By Novel Bacteria Isolated From Burrow Sediments Of Marine Benthic Macrofauna, Wai Ki Chung

Electronic Theses and Dissertations

Polycyclic aromatic hydrocarbons (PAH) are significant environmental pollutants and represent a severe health threat. Many cosmopolitan areas including coastal environments are heavily impacted by PAH. This research investigates the isolation of PAH-degrading bacteria from burrow sediment of marine benthic macrofauna and their potential in bioremediation. Macrofaunal burrow sediment is biogeochemically distinct from bulk sediment and has high microbial activities. Samples were collected from a local uncontaminated cove and PAH degradation potential was measured by incubating sediment slurries with exogenous PAH. Burrow sediments from two polychaetes and a mollusc showed significantly higher PAH degradation potential than the bulk sediment. The degradation …

Meeting Medical Terminology Needs: The Ontology-Enhanced Medical Concept Mapper, Gondy Leroy, Hsinchun Chen

CGU Faculty Publications and Research

This paper describes the development and testing of the Medical Concept Mapper, a tool designed to facilitate access to online medical information sources by providing users with appropriate medical search terms for their personal queries. Our system is valuable for patients whose knowledge of medical vocabularies is inadequate to find the desired information, and for medical experts who search for information outside their field of expertise. The Medical Concept Mapper maps synonyms and semantically related concepts to a user's query. The system is unique because it integrates our natural language processing tool, i.e., the Arizona (AZ) Noun Phraser, with human-created …

Review Of Family Experience With Mental Illness. Richard Tessler And Gail Gamache. Reviewed By James W. Callicutt, James W. Callicutt

The Journal of Sociology & Social Welfare

Book review of Richard Tessler and Gail Gamache, Family Experiences with Mental Illness. Westport, CT: Auburn House, 2000. $19.95 papercover.