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Articles 1 - 11 of 11
Full-Text Articles in Medicine and Health Sciences
Comparison Of The Quantification Of Caffeine In Human Plasma By Gas Chromatography And Elisa, A. B. Carregaro, W. E. Woods, Thomas Tobin, A. Queiroz-Neto
Comparison Of The Quantification Of Caffeine In Human Plasma By Gas Chromatography And Elisa, A. B. Carregaro, W. E. Woods, Thomas Tobin, A. Queiroz-Neto
Veterinary Science Faculty Publications
In the present study we evaluated the precision of the ELISA method to quantify caffeine in human plasma and compared the results with those obtained by gas chromatography. A total of 58 samples were analyzed by gas chromatography using a nitrogen-phosphorus detector and routine techniques. For the ELISA test, the samples were diluted to obtain a concentration corresponding to 50% of the absorbance of the standard curve. To determine whether the proximity between the I50 of the standard curve and that of the sample would bring about a more precise result, the samples were divided into three blocks according …
Determination Of Antibody Production By Ifat And Elisa In Rainbow Trout (Oncorhynchus Mykiss) Immunized By Yersinia Ruckeri Bacterin, Ayşegül Kubi̇lay, Gülşen Ti̇mur
Determination Of Antibody Production By Ifat And Elisa In Rainbow Trout (Oncorhynchus Mykiss) Immunized By Yersinia Ruckeri Bacterin, Ayşegül Kubi̇lay, Gülşen Ti̇mur
Turkish Journal of Veterinary & Animal Sciences
In this study, the induced antibody level of rainbow trout immunized by Yersinia ruckeri bacterin was determined using two serological techniques, namely, indirect fluorescent antibody (IFAT) and enzyme-linked immunosorbent assay (ELISA). The induced humoral antibody levels were studied after intraperitonal injection of Yersinia ruckeri bacterin inoculates containing Freund's incomplete adjuvant or PBS in rainbow trout during the experimental period of 20 weeks. The control and experimental groups each contained 20 fish. The group which was immunized by the PBS containing bacterin inoculate received a booster injection. Experiments were carried out in 11-12°C water. Low antibodies titres were detected by ELISA …
Seroprevalance Of Babesia Ovis In Sheep In Malatya, Müni̇r Aktaş, Ali̇ Düzgün, Cahi̇t Babür
Seroprevalance Of Babesia Ovis In Sheep In Malatya, Müni̇r Aktaş, Ali̇ Düzgün, Cahi̇t Babür
Turkish Journal of Veterinary & Animal Sciences
This study was carried out in Malatya and vicinity to determine the seroprevalance of Babesia ovis in sheep. A total of 220 sheep sera were tested against B. ovis using the Enzyme-Linked Immunosorbent Assay (ELISA). In addition, peripheral blood smears from these sheep were prepared and examined for B. ovis. The ELISA test showed that 55.9% of sheep were seropositive for B. ovis infection. The infection rate was 49.2% in sheep which were 1-12 months old and 59% in sheep which were older than one year. In the microscopic examination of blood smears, four out of 220 sheep were found …
Production Of Monoclonal Antibody Specific For Human Hemoglobin, Selma Öztürk, Beyazit Çirakoğlu
Production Of Monoclonal Antibody Specific For Human Hemoglobin, Selma Öztürk, Beyazit Çirakoğlu
Turkish Journal of Medical Sciences
Hemoglobin is not normally present in sputum, urine or feces except in minute amounts. Demonstration of the presence of hemoglobin in these materials is an important diagnostic clue that usually implies bleeding within the respiratory, urinary or gastroentestinal tract, respectively. In this work, a hybridoma line secreting monoclonal antibody (Mab) highly specific for human hemoglobin A (hHbA) was generated by fusion of immunized BALB/c mouse splenic lymphocytes with non-secreting mouse myeloma cells followed by selection in hypoxanthine-aminopterin-thymidine (HAT) medium and the screening of culture fluids for hHb A affinity in ELISA. The Mab produced by MAM 2/3B6 is highly specific …
Sandwich-Type Enzyme Immunoassay For Amyloid A4 Protein In Cerebrospinal Fluid From Patients With Head Trauma, İbrahi̇m Pi̇ri̇m
Sandwich-Type Enzyme Immunoassay For Amyloid A4 Protein In Cerebrospinal Fluid From Patients With Head Trauma, İbrahi̇m Pi̇ri̇m
Turkish Journal of Medical Sciences
The aim of this study was to design a simple method for detecting the amyloid A4 protein in the cerebrospinal fluid (CSF) of patients who had severe brain trauma caused by traffic or other accidents and to evaluate its diagnostic and prognostic value. For this purpose, a sandwich type ELISA assay was used. A4, a 4kDa protein of 39-43 amino acids, is a metabolic product of a membrane-spanning, 695-770 aa, precursor molecule, the b-amyloid precursor protein (b-APP). The polyclonal rabbit antibody against synthetic A4 protein (1-40 residues) was used as an immobilized antibody. A mouse monoclonal antibody against synthetic A4 …
The Diagnosis Of Antibodies Against Newcastle Disease Virus By Hi And Elisa Tests In Vaccinated Chickens, Nejat Aydin, Hakan Yardimci, K. Semi̇h Gümüşsoy, Belgi̇n Erdem
The Diagnosis Of Antibodies Against Newcastle Disease Virus By Hi And Elisa Tests In Vaccinated Chickens, Nejat Aydin, Hakan Yardimci, K. Semi̇h Gümüşsoy, Belgi̇n Erdem
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine serum antibody levels against the Newcastle disease virus in vaccinated chicken flocks in addition to experimental animals by hemagglutination inhibition (HI)test and enzyme linked immunosorbent assay (ELISA) and compare the results. Methanol-precipitated antigen prepared from the LaSota strain of Newcastle disease virus was used with peroxidase-labelled rabbit anti-chicken IgG as a conjugate and ortho-phenylenediamine as a substrate in ELISA. The negative threshold was found to be an optical density of 0.19 (OD)at a 490-nm wavelength.In addition to the 50 experimental animals, a total of 504 chicken sera (broiler,layer and breeder flocks)collected from …
Comparison Of Elisa With Other Serological Tests In The Diagnosis Of Ovine Brucellosis, Hasan Öngör, Adi̇le Muz, Burhan Çeti̇nkaya
Comparison Of Elisa With Other Serological Tests In The Diagnosis Of Ovine Brucellosis, Hasan Öngör, Adi̇le Muz, Burhan Çeti̇nkaya
Turkish Journal of Veterinary & Animal Sciences
In this study,antibodies against Brucella melitensis in aborted sheep were investigated with enzyme-linked immunosorbent assay (ELISA), standard tube agglutination test (SAT), rose bengal plate test (RBPT), complement fixation test (CFT), 2-mercaptoethanol microagglutination test (2-ME-MAT)and dithiothreitol microagglutination test (DTT-MAT). The sensitivity and specificity of the tests were calculated by using CFT results as the gold standard. A Kappa value was used to measure the agreements between ELISA and the other tests. Sonicated antigen prepared from Brucella melitensis 16M strain,the peroxidase labelled anti-sheep IgG conjugate and ortho-phenylenediamine substrate were used in ELISA. A total of 500 blood samples collected from 36 sheep …
Seroprevalence Of Babesia Ovis In Awassi Sheep In Urfa, Turkey, Zi̇şan Emre, Ali̇ Düzgün, Mehmet İri̇adam, Hati̇ce Sert
Seroprevalence Of Babesia Ovis In Awassi Sheep In Urfa, Turkey, Zi̇şan Emre, Ali̇ Düzgün, Mehmet İri̇adam, Hati̇ce Sert
Turkish Journal of Veterinary & Animal Sciences
Seroprevalence of Babesia ovis in Awassi sheep was studied in Urfa and environs between November 1997 and October 1998. A total of 607 serum samples were collected from sheep in 12 different localities, and tested for the presence of antibodies to B. ovis by using an indirect ELISA. Blood smears were also prepared from 110 sheep. The overall prevalence of B. ovis infection was 41.02%. B. ovis was only detected in two blood smears examined (1.82%). A total of 86 ticks were collected from the sheep during the study. Of the ticks examined, 17.44% were Rhipicephalus turanicus, 54.65% were Haemophysalis …
Diagnosis Of Lymphoid Leukosis (Ll) By Elisa (Enzyme-Linked Immunosorbent Assay) And Marek's Disease (Md) By If (Immunofluorescence) Test In Chickens, Selda Özbi̇lgi̇n, Ayşi̇n Şen, Mi̇hri̇ban Ülgen, K. Tayfun Çarli, Gürsel Sönmez, Özlem Özmen
Diagnosis Of Lymphoid Leukosis (Ll) By Elisa (Enzyme-Linked Immunosorbent Assay) And Marek's Disease (Md) By If (Immunofluorescence) Test In Chickens, Selda Özbi̇lgi̇n, Ayşi̇n Şen, Mi̇hri̇ban Ülgen, K. Tayfun Çarli, Gürsel Sönmez, Özlem Özmen
Turkish Journal of Veterinary & Animal Sciences
In this study, our aim was to diagnose lymphoid leukosis and Marek's disease, which are neoplastic diseases of chickens, by ELISA and Indirect Immunofluorescence Test (IIF), respectively. Samples (eggs and tissues) were selectively taken from the flocks suspected of having lymphoid leukosis and Marek's disease. The tissues were macroscopically and microscopically examined. Tissues belonging to some flocks showed typical lesions for lymphoid leukosis and Marek's disease. Seven broiler breede flocks and three commercial layer flocks were tested for avian leukosis virus (ALV) group specific antigen (p27) in the albumen. ALV p27 antigen was detected in five out of ten flocks. …
Detection Of Antibodies Against Salmonella Enteritidis In Chicken Serum And Egg Yolk With Elisa, Gülay Altay, Hakan Yardimci
Detection Of Antibodies Against Salmonella Enteritidis In Chicken Serum And Egg Yolk With Elisa, Gülay Altay, Hakan Yardimci
Turkish Journal of Veterinary & Animal Sciences
A total of 473 sera from hens of different age groups and different breeds and a total of 430 eggs were collected from 11 breeders and egg producers in the districts of Adapazarı, Afyon, Ankara and Konya. Lipopolysaccharide (LPS), heat-extract (HE) and flagella antigens were tested in the standardizing ELISA technique. Flagella antigen was found useful in the screening of field samples. Of the 48 (10.2%) rapid plate agglutination positive sera, 7 were negative in ELISA. One hundred and seventy-three (36.6%) of the 473 sera and 76 (17.7%) of the 430 egg yolks were found to be positive with ELISA. …
Comparison And Evaluation Of The Passive Haemagglutination Test And Elisa In Routine Screening Of Contagious Equine Metritis (Cem) In Mares With Endometritis Or Infertility, N. Yakut Özgür, Masashi Eguchi, Toru Anzai, Serkan İki̇z, Ati̇lla Ilgaz
Comparison And Evaluation Of The Passive Haemagglutination Test And Elisa In Routine Screening Of Contagious Equine Metritis (Cem) In Mares With Endometritis Or Infertility, N. Yakut Özgür, Masashi Eguchi, Toru Anzai, Serkan İki̇z, Ati̇lla Ilgaz
Turkish Journal of Veterinary & Animal Sciences
One hundred and twenty thoroughbred breeder mares with endometritis or infertility were investigated for the detection of antibodies to Taylorella equigenitalis by the passive haemagglutination (PHA) test and ELISA between 1997 and 1999 in the Marmara region and the two tests were compared. Fifty-eight (48.33 %) of 120 sera were found to be positive by the PHA test and 52 (43.33%) by ELISA. 22 of the sera were found to be positive by the PHA test reacted at 1/64 titer, 27 at 1/128, 7 at 1/256 and 2 at 1/512 titer. Sera from 44 mares were positive in both tests. …