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Articles 931 - 960 of 2525
Full-Text Articles in Medicine and Health Sciences
Critical Factors On Chemical Properties And Heavy Metals In Water For Livestock Farms In Thailand, Teeraporn Makaroon, Suwicha Kasemsuwan, Nattavut Ratanavanichrojn, Hathairad Hananantachai, Rachaneekorn Mingkhwan, Sutha Khaodhiar, Phitsanu Tulayakul
Critical Factors On Chemical Properties And Heavy Metals In Water For Livestock Farms In Thailand, Teeraporn Makaroon, Suwicha Kasemsuwan, Nattavut Ratanavanichrojn, Hathairad Hananantachai, Rachaneekorn Mingkhwan, Sutha Khaodhiar, Phitsanu Tulayakul
The Thai Journal of Veterinary Medicine
This study aimed to investigate chemical quality of animal drinking used in livestock farms in Thailand. Samples and information from 68 pig farms, 35 chicken farms and 9 duckling farms were collected during March to August 2011. Water quality data were analyzed and compared with standard levels using the Mann-Whitney U and Wilcoxon Signed Rank Tests. Results revealed that median chemical values of water for livestock were generally within standard levels, except manganese and iron, which were detected in surfacewater with median values of 0.183 and 0.506 ppm, respectively, and are both higher than standard limits for drinking water. Median …
Analysis Of Mirna-29a Expression In Porcine Peripheral Blood Mononuclear Cells Using Quantitative Reverse Transcription Polymerase Chain Reaction, Sunaree Nantakhruea, Porntippa Lekcharoensuk, Win Surachetpong
Analysis Of Mirna-29a Expression In Porcine Peripheral Blood Mononuclear Cells Using Quantitative Reverse Transcription Polymerase Chain Reaction, Sunaree Nantakhruea, Porntippa Lekcharoensuk, Win Surachetpong
The Thai Journal of Veterinary Medicine
MicroRNAs (miRNAs) are small non coding RNA that regulate gene expression as post transcriptional regulators in mammalian tissues. One of the miRNAs that participates in mammalian immune response is miR-29a. Despite the limited information of miR-29a in porcine cells, it is interesting to determine whether miR-29a involves in immune regulation in pig. In this study, we developed a quantitative reverse transcription polymerase chain reaction using SYBR detection system to examine the expression of primary miR-29a (pri-miR-29a) in porcine peripheral blood mononuclear cells (PBMCs) in the presence of mitogen. PBMCs were stimulated with concanavalin A (Con A) at a concentration of …
Field Efficacy Of Potassium Peroxymonosulfate (Pms) Oxidizing Disinfectant (Virusniptm) Against Porcine Circovirus Type 2 (Pcv2) In Gilt Acclimatization Unit, Korakrit Poonsuk, Jirapat Arunorat, Sasiwimon Talummuk, Rapeepat Kunalintip, Woravit Anuvongnukroh, Roongroje Thanawongnuwech
Field Efficacy Of Potassium Peroxymonosulfate (Pms) Oxidizing Disinfectant (Virusniptm) Against Porcine Circovirus Type 2 (Pcv2) In Gilt Acclimatization Unit, Korakrit Poonsuk, Jirapat Arunorat, Sasiwimon Talummuk, Rapeepat Kunalintip, Woravit Anuvongnukroh, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
Virusnip™ is one of the commercial potassium peroxymonosulfate disinfectants widely used in swine farms. This disinfectant is well studied and proves to be excellent in antimicrobial activity previously. However, there is no information about its efficacy under field condition. Thus, the objective of this study was to determine the virucidal activity of Virusnip® against PCV2 in farm condition. Consequently, 1:200 concentration of VirusnipTM was sprayed for 15 minutes once or twice daily depending on the acclimatization period in gilts isolation units compared to the untreated group in the same building. Environmental swabs and oral fluid samples were collected and pooled …
Molecular Characterization Of Wenyonella Philiplevinei From Ducks In Hunan Province, China, Hui Lan Wu, Peng Hui Hu, Jin Zhang
Molecular Characterization Of Wenyonella Philiplevinei From Ducks In Hunan Province, China, Hui Lan Wu, Peng Hui Hu, Jin Zhang
The Thai Journal of Veterinary Medicine
Wenyonella philiplevinei is one of the important pathogens of duck coccidiasis. In the present study, a portion of the 18S rDNA was amplified from W. philiplevinei by Polymerase chain reaction (PCR), cloned and then sequenced. The sequences of 18S rDNA of all samples were 422 bp in size. A + T content of the 18S rDNA sequences was 58-59%. Sequence comparison revealed that the similarity in 18S rDNA sequences between Hunan isolates and W. philiplevinei available (Guangdong isolate) was more than 98%. Intra-specific sequence variations within each of the Hunan isolates were 0-1.7%. However, the inter-specific sequence differences between W. …
Molecular Characterization Of A Genetically Diverse Bubaline Picobirnavirus Strain, India, Yashpal Singh Malik, Naveen Kumar, Kuldeep Sharma, Amit Kumar Sharma, Subhankar Sircar, Lalit M. Jeena, Neeraj K. Singh, Anjan Mondal, Siddhartha N. Joardar, Ganesh Balasubramanian
Molecular Characterization Of A Genetically Diverse Bubaline Picobirnavirus Strain, India, Yashpal Singh Malik, Naveen Kumar, Kuldeep Sharma, Amit Kumar Sharma, Subhankar Sircar, Lalit M. Jeena, Neeraj K. Singh, Anjan Mondal, Siddhartha N. Joardar, Ganesh Balasubramanian
The Thai Journal of Veterinary Medicine
Picobirnavirus (PBV) associated gastrointestinal infections have been noticed in humans as well as in a number of animal species, however, to the best of our information, report about the detection and characterisation of PBV in buffaloes is not presented. In this study, RNA polymerase (RdRp) gene targeted RT-PCR assay was used to recognize PBVs and additionally the isolate was analysed for evolutionary and sequence homology. The buffalo PBV isolate was of genogroup I (GG I) nature and a comparative sequence analysis revealed homology of 44.5% and 45.1% at nucleotide and amino acid levels, respectively, with the prototype human PBV strain …
Ecg Quiz, Chollada Buranakarl, Anusak Kijtawornrat, Thanusorn Phakhawambodee
Ecg Quiz, Chollada Buranakarl, Anusak Kijtawornrat, Thanusorn Phakhawambodee
The Thai Journal of Veterinary Medicine
No abstract provided.
Ultrasound Diagnosis, Phiwipha Kamonrat
Ultrasound Diagnosis, Phiwipha Kamonrat
The Thai Journal of Veterinary Medicine
No abstract provided.
Effects Of Environmental Factors, Ages And Breeds On Semen Characteristics In Thai Indigenous Chickens: A One-Year Study, Pornjit Sonseeda, Thevin Vongpralub, Banyat Laopaiboon
Effects Of Environmental Factors, Ages And Breeds On Semen Characteristics In Thai Indigenous Chickens: A One-Year Study, Pornjit Sonseeda, Thevin Vongpralub, Banyat Laopaiboon
The Thai Journal of Veterinary Medicine
Several reports on the semen characteristics of domestic fowl have indicated that environmental factors, breed and age significantly affect semen quality. There is, however, a paucity of data on effects of environmental factor, breed and age on semen characteristic in Thai indigenous chickens. The aims of the present study were to investigate the effects of annual ambient temperature and humidity, local breeds (i.e. Lueng hang kaow, Pradoo hang dam and Chee), and ages (10 and 18 months) on sperm production and semen quality among Thai native cocks. Thirty-six Thai native cocks were housed under natural environmental conditions, and semen was …
Endoscopic Evaluation Of Gastric Mucosa To Determine Safety Of Three Chondroprotective Drugs In Healthy Dogs, Korakot Nganvongpanit, Kittipong Kungprathum, Terdsak Yano, Kumpanart Soontornvipart
Endoscopic Evaluation Of Gastric Mucosa To Determine Safety Of Three Chondroprotective Drugs In Healthy Dogs, Korakot Nganvongpanit, Kittipong Kungprathum, Terdsak Yano, Kumpanart Soontornvipart
The Thai Journal of Veterinary Medicine
This randomized, double-blind, placebo-controlled study investigated the effects of glucosamine/chondroitin sulfate, chondroitin sulfate, and diacerein on the gastric mucosa of dogs, and on clinical signs of vomiting and diarrhea. Twenty-one healthy adult dogs were included in the study, and were randomly assigned to seven treatment groups, 3 dogs per group. The effect of those medicines on the gastric mucosa was evaluated by endoscopy on day 3 and day 14, and compared with pre-treatment. Clinical signs, including vomiting and diarrhea, were recorded every day during the study period. The results showed a non-significant effect of glucosamine/chondroitin sulfate, chondroitin sulfate, and diacerein …
Surgical Removal Of Urethral And Bladder Stones In Female Asian Elephant (Elephas Maximus) By Episiotomy And Urethrotomy, Nikorn Thongtip, Benjapol Lorsanyaluck, Manakorn Sukmak, Sirinart Chaichanathong, Naris Thengchaisri, Pornchai Sunyathitiseree, Worawidh Wajjwalku
Surgical Removal Of Urethral And Bladder Stones In Female Asian Elephant (Elephas Maximus) By Episiotomy And Urethrotomy, Nikorn Thongtip, Benjapol Lorsanyaluck, Manakorn Sukmak, Sirinart Chaichanathong, Naris Thengchaisri, Pornchai Sunyathitiseree, Worawidh Wajjwalku
The Thai Journal of Veterinary Medicine
A 45-year-old female Asian elephant (Elephas maximus) was presented with urinary incontinence and solid masses at the perineal area. Vaginoscopy revealed stricture of the vaginal vestibule. Trans skin and rectal ultrasonography demonstrated presence of hyperechogenic uroliths. Episiotomy and urethrotomy were performed after standing sedation with xylazine and perineal infiltration with lidocaine. Total of 8 kg urethral calculi were retrieved manually. Calcium carbonate was identified as the main component of the stones. Due to acute urinary obstruction after the operation, an episiotomy was left open allowing urine to pass through the incision. Following urine scalding, necrotic tissues were detected along the …
Genetic Diversity Of Mycoplasma Hyosynoviae Field Isolates In Thailand, Pacharee Thongkamkoon, Sommai Yuwapanichsampan, Hideki Kobayashi, Pornpen Pathanasophon, Masato Kishima, Koshi Yamamoto
Genetic Diversity Of Mycoplasma Hyosynoviae Field Isolates In Thailand, Pacharee Thongkamkoon, Sommai Yuwapanichsampan, Hideki Kobayashi, Pornpen Pathanasophon, Masato Kishima, Koshi Yamamoto
The Thai Journal of Veterinary Medicine
Pulsed-field gel electrophoresis (PFGE) and Random amplified polymorphic DNA (RAPD) analyses were performed to compare techniques and to investigate genetic diversity as an epidemiological data of Thai M. hyosynoviae isolates. A total of 42 isolates including a type strain S16 was typable and consisted of 39 different patterns by RAPD technique, whereas 37 isolates (97%) were typable and consisted of 22 different patterns by PFGE technique. Based on PFGE patterns, multiple clones of M. hyosynoviae were generally present in pig farms, whereas high genetic heterogeneity of M. hyosynoviae among the pig farms was shown. No identical PFGE pattern between the …
Viability And Growth Of Preantral Follicles Derived From Cryopreserved Ovarian Tissues Of A Cheetah (Acinonyx Jubatus) Post-Mortem, Grisnarong Wongbandue, Nae Tanpradit, Daraka Thongthainun, Paweena Thuwanut, Kaywalee Chatdarong
Viability And Growth Of Preantral Follicles Derived From Cryopreserved Ovarian Tissues Of A Cheetah (Acinonyx Jubatus) Post-Mortem, Grisnarong Wongbandue, Nae Tanpradit, Daraka Thongthainun, Paweena Thuwanut, Kaywalee Chatdarong
The Thai Journal of Veterinary Medicine
This study aimed to investigate freezing effects of ovarian tissues on survival of preantral follicles and observing in vitro growing of preantral follicles retrieved from cryopreserved ovarian cortical tissues of a cheetah post-mortem. After 29-hour cold storage, ovarian cortices were cut into small pieces (2.0 x 2.0 x 1.0 mm3) and allocated to be frozen using a passive cooling container (n = 3 pieces) or vitrification (n=3 pieces). After one year of storage, 43 (10/23) and 21% (12/58) follicles isolated from ovarian tissues cryopreserved using a passive cooling device (slow freezing rate) and vitrification, respectively, were viable (positively stained with …
Chemical Immobilization Of Bornean Leopard Cats (Prionailurus Bengalensis Borneoensis) With Tiletamine And Zolazepam Under Field Conditions In Borneo, Nájera Fernando, Cediel-Algovia Rafael, Hearn Andrew, Ross Jo, Dench Rosalie, Alcázar Paloma, Nathan Senthivel, Iñaki De Gaspar, Revuelta Luis
Chemical Immobilization Of Bornean Leopard Cats (Prionailurus Bengalensis Borneoensis) With Tiletamine And Zolazepam Under Field Conditions In Borneo, Nájera Fernando, Cediel-Algovia Rafael, Hearn Andrew, Ross Jo, Dench Rosalie, Alcázar Paloma, Nathan Senthivel, Iñaki De Gaspar, Revuelta Luis
The Thai Journal of Veterinary Medicine
Nine wild Bornean leopard cats were anesthetized using a combination of tiletamine and zolazepam (Zoletil©) after being captured in humanely-designed live traps in Sabah, Malaysian Borneo, for the purpose of fitting radio-collars. For five leopard cats (group 1) a single dose of 6.92±1.06 mg/kg of Zoletil© was administered. The mean induction time from the initial Zoletil© dose was 7.9 ± 1.77 minutes, and the mean anesthesia time was 47.2 ± 25.1. For 4 leopard cats (group 2) after an initial mean dose of 6.92±1.06 mg/kg of Zoletil©, it was necessary to administer a second dose (or booster) of Zoletil© (mean …
Investigation Into Bacillus Anthracis Spore In Soil And Analysis Of Environmental Parameters Related To Repeated Anthrax Outbreak In Sirajganj, Bangladesh, Md. Murshidul Ahsan, Mohammad Ferdousur Rahman Khan, Md. Bahanur Rahman, Shah Md. Ziqrul Haq Chowdhury, Md. Shafiullah Parvej, Mueena Jahan, K. H. M. Nazmul Hussain Nazir
Investigation Into Bacillus Anthracis Spore In Soil And Analysis Of Environmental Parameters Related To Repeated Anthrax Outbreak In Sirajganj, Bangladesh, Md. Murshidul Ahsan, Mohammad Ferdousur Rahman Khan, Md. Bahanur Rahman, Shah Md. Ziqrul Haq Chowdhury, Md. Shafiullah Parvej, Mueena Jahan, K. H. M. Nazmul Hussain Nazir
The Thai Journal of Veterinary Medicine
The study was conducted for the isolation and detection of Bacillus anthracis spores from soil collected from Sirajganj district (a north-western district of Bangladesh), and to assess the parameters that may relate to the repeated anthrax outbreak. A total of 48 soil samples were collected from the study area during January to November 2012. Endospores were extracted from soil and the Bacillus anthracis was identified using conventional bacteriological, biochemical and sensitivity test against Penicillin-G. The viable B. anthracis spores could be detected from 14 (29.17%) soil samples. Moisture content, pH, calcium and organic carbon contents of the soils were measured …
Buccal Swab As A Source Of Noninvasive Technique For Genomic Dna Collection In Felidae, Janjira Phavaphutanon, Sudtisa Laopium, Kawin Nanklang, Kaikanoke Sirinarumitr, Kornchai Kornkaewrat, Anuchai Pinyopummin, Jitrakorn Viriyarumpa, Piyawan Suthanmapinunt, Narathip Vorawattanatham
Buccal Swab As A Source Of Noninvasive Technique For Genomic Dna Collection In Felidae, Janjira Phavaphutanon, Sudtisa Laopium, Kawin Nanklang, Kaikanoke Sirinarumitr, Kornchai Kornkaewrat, Anuchai Pinyopummin, Jitrakorn Viriyarumpa, Piyawan Suthanmapinunt, Narathip Vorawattanatham
The Thai Journal of Veterinary Medicine
This study was conducted to determine whether buccal cells obtained by swabbing could be a DNA source for genetic analysis, and to compare two preservative media, distilled water and Tris-EDTA (TE), pH 8.0. Buccal cell samples were collected from 30 domestic cats (Felis catus), 4 captive fishing cats (Prionailurus viverrinus) and 8 captive tigers (Panthera tigris). The total concentration of DNA and purity were not statistically different between the preservative media. All samples could be graded as a minimum as high quality DNA (DNA found with A260/A280 ratio >2.0 or < 1.8). In addition, the quality of DNA extracted from both media was successfully amplified for PCR. However, when compared to whole blood samples, total DNA concentration from buccal swab was significantly lower (p < 0.05) .Despite the decrease in DNA yield, buccal swab could be an alternative source for obtaining DNA for genetic analyses. This non-invasive, simple and inexpensive technique eases genetic studies in this family especially in domestic cat from which whole blood samples are difficult to collect.
Ultrasound Diagnosis, Phiwipha Kamonrat
Ultrasound Diagnosis, Phiwipha Kamonrat
The Thai Journal of Veterinary Medicine
No abstract provided.
Discrimination Between Tropical Bed Bug Cimex Hemipterus And Common Bed Bug Cimex Lectularius (Hemiptera: Cimicidae) By Pcr-Rflp, Apiwat Tawatsin, Kittitouch Lorlertthum, Atchara Phumee, Usavadee Thavara, Jotika Boon-Long, Rungfar Boonserm, Padet Siriyasatien
Discrimination Between Tropical Bed Bug Cimex Hemipterus And Common Bed Bug Cimex Lectularius (Hemiptera: Cimicidae) By Pcr-Rflp, Apiwat Tawatsin, Kittitouch Lorlertthum, Atchara Phumee, Usavadee Thavara, Jotika Boon-Long, Rungfar Boonserm, Padet Siriyasatien
The Thai Journal of Veterinary Medicine
Bed bugs, Cimex hemipterus and Cimex lectularius, are common blood-sucking ectoparasites of human and currently found in many countries around the world. In Thailand, both species have been found mostly in hotels in tourist attraction areas and the insecticide resistance of these insects was also documented. To date, identification of these two bed bug species is based on morphological taxonomy, a technique which requires expertise and in some instance is difficult especially for immature bed bugs or incomplete bed bug samples. In this study, we analyzed the cytochrome c oxidase subunit I (COI) gene of bed bugs, C. hemipterus and …
Detection Of Genetic Variations Using Rapd Markers In Siberian Huskies Affected With Swimming Puppy Syndrome, Siriwadee Chomdej, Arphaphorn Dokphut, Waranee Pradit, Korakot Nganvongpanit
Detection Of Genetic Variations Using Rapd Markers In Siberian Huskies Affected With Swimming Puppy Syndrome, Siriwadee Chomdej, Arphaphorn Dokphut, Waranee Pradit, Korakot Nganvongpanit
The Thai Journal of Veterinary Medicine
Swimming puppy syndrome (SPS) is a curious disease found in dogs. The causes and pathology of this disease are still unknown, although heredity is usually considered to be one of the underlying factors. The objective of this study was to investigate the association between genetics and SPS in a Siberian husky model. Four Siberian husky puppies diagnosed with SPS were subjects of this study, while three other healthy Siberian husky puppies served as controls. Blood samples were collected for DNA isolation, using random amplified polymorphic DNA (RAPD) technique with 16 random primers. No genetic variation was found between affected puppies …
Ecg Quiz, Chollada Buranakarl, Suwanakiet Sawangkoon, Anusak Kijtawornrat, Thanusorn Phakhawambodee
Ecg Quiz, Chollada Buranakarl, Suwanakiet Sawangkoon, Anusak Kijtawornrat, Thanusorn Phakhawambodee
The Thai Journal of Veterinary Medicine
No abstract provided.
Ophthalmology Snapshot, Nalinee Tuntivanich
Ophthalmology Snapshot, Nalinee Tuntivanich
The Thai Journal of Veterinary Medicine
No abstract provided.
What Is Your Diagnosis, Pranee Tuntivanich, Suwicha Chuthatep1
What Is Your Diagnosis, Pranee Tuntivanich, Suwicha Chuthatep1
The Thai Journal of Veterinary Medicine
No abstract provided.
Bone Cell Function: A Review, Hoai Nam Nguyen, Naruepon Kampa
Bone Cell Function: A Review, Hoai Nam Nguyen, Naruepon Kampa
The Thai Journal of Veterinary Medicine
Bone is dynamic tissue which is continuously formed and absorbed by its own cells in response to stimuli such as hormones, mechanical loading and growth factors. Understanding the function of bone cells is important, not only in orthopedic field but also in research study involving bone. Bone cells work in harmony to maintain a balance between bone formation and resorption, ultimately to control bone structure and function. Osteoblasts are cells, which contribute to deposition of organic components of bone extracellular matrix. They control recruitment, differentiation and maturation of osteoclasts that participate in resorption activity. In addition, osteoclasts associated with bone …
Short Term Echocardiographic And Clinical Effects Of Ramipril On Dogs With Asymptomatic Degenerative Mitral Valve Disease, Prakit Kohkayasit, Sirilak Surachetpong
Short Term Echocardiographic And Clinical Effects Of Ramipril On Dogs With Asymptomatic Degenerative Mitral Valve Disease, Prakit Kohkayasit, Sirilak Surachetpong
The Thai Journal of Veterinary Medicine
Angiotensin converting enzyme (ACE) inhibitors have beneficial effects on degenerative mitral valve disease (DMVD) dogs with stages C and D (ACVIM classification) and on dogs with congestive heart failure. However, ACE inhibitors’ effects on stage B2 or asymptomatic DMVD dogs have still been uncertain. Ramipril is an ACE inhibitor that has lipophilic effects and can suppress ACE in cardiac tissue effectively. We hypothesized that ramipril had beneficial effects on dogs with naturally occurring DMVD in stage B2. Twenty dogs with stage B2 DMVD, weighing between 3-12 kg and being older than 6 years, were recruited into the study. The dogs …
Development Of Enzyme-Linked Immunosorbent Assay To Detect Antibodies Against Chicken Infectious Anemia Virus, Niwat Chansiripornchai, Sangkae Pongthanes, Piyarat Chansiripornchai, Wisanu Wanasawaeng
Development Of Enzyme-Linked Immunosorbent Assay To Detect Antibodies Against Chicken Infectious Anemia Virus, Niwat Chansiripornchai, Sangkae Pongthanes, Piyarat Chansiripornchai, Wisanu Wanasawaeng
The Thai Journal of Veterinary Medicine
standardized and robust indirect enzyme-linked immunosorbent assay (ELISA) to detect antibodies against chicken infectious anemia virus (CIAV) was developed. Thai CIAV was isolated and used for the entirety of this study. For virus cultivation, a cell density of MDCC-MSB1 cells at 3 x 106 cells/ml and a fetal bovine serum concentration at 10 percent were the most appropriate for Thai CIAV. Then, crude virus of CIAV was prepared using ultracentrifugation technique. To develop the ELISA test, optimal dilution of goat anti-chicken IgG was 1 : 200 and antigen concentration in the ELISA was 1.00 μg/ml, as determined by checkerboard titration. …
Effect Of Improved Cooling On Daily Rhythmicity Of Body Temperature In Cross-Bred Holstein Dairy Cows Under Tropical Conditions, Siriwat Suadsong, Thuchadaporn Chaikhun, Junpen Suwimonteerabutr
Effect Of Improved Cooling On Daily Rhythmicity Of Body Temperature In Cross-Bred Holstein Dairy Cows Under Tropical Conditions, Siriwat Suadsong, Thuchadaporn Chaikhun, Junpen Suwimonteerabutr
The Thai Journal of Veterinary Medicine
The objectives of this experiment were to study the effect of heat stress on daily rhythmicity of body temperature in dairy cows under hot and humid climatic conditions and to evaluate the efficacy of improved cooling system by monitoring body temperature. Sequential measurements of body temperature, air temperature, relative humidity and THI were monitored every 5 min. Four cross-bred Holstein-Frisian (93.75% HF) cows were assigned randomly to be used in two trial periods to determine the effects of heat stress and improved cooling system on body temperature. The experiment was conducted in the barn for a total of 6 days, …
Acute Pulmonary Toxicity Caused By Single Intratracheal Instillation Of Various Doses Of Colloidal Silver Nanoparticles In Mice: Pathological Changes, Particle Bioaccumulation And Metallothionien Protein Expression, Theerayuth Kaewamatawong, Wijit Banlunara, Pattwat Maneewattanapinyo, Chuchaat Thammacharoen, Sanong Ekgasit
Acute Pulmonary Toxicity Caused By Single Intratracheal Instillation Of Various Doses Of Colloidal Silver Nanoparticles In Mice: Pathological Changes, Particle Bioaccumulation And Metallothionien Protein Expression, Theerayuth Kaewamatawong, Wijit Banlunara, Pattwat Maneewattanapinyo, Chuchaat Thammacharoen, Sanong Ekgasit
The Thai Journal of Veterinary Medicine
To study acute lung toxicity of various doses of colloidal silver nanoparticles (Ag-NPs), mice were intratracheally instilled with 0, 10, 100, 1000 or 10,000 ppm of Ag-NPs. Histopathology, autometallography (AMG) and immunohistochemistry were determined at 1, 3, 7 and 15 days post-exposure.Instillation of 100, 1,000 and 10,000 ppm Ag-NPs produced moderate to severe necrotizing bronchitis and alveolitiswith hypertrophy and hyperplasia of alveolar epithelial cells. The severity of the pulmonary inflammation and damage increased in a dose-dependent manner. Concomitant lamininimmunohistochemical findings generally correlated with pulmonary lesions. Interleukin 1- beta (IL-1β) and tumor necrotic factor-alpha (TNF-α) positive immunostaining were found in the …
Efficacy Of Different Vaccination Programs Against Velogenic Newcastle Disease Virus Challenged In 28-Day-Old Broiler Chickens, Tawatchai Pohuang, Nida Sirikobkul, Jiroj Sasipreeyajan
Efficacy Of Different Vaccination Programs Against Velogenic Newcastle Disease Virus Challenged In 28-Day-Old Broiler Chickens, Tawatchai Pohuang, Nida Sirikobkul, Jiroj Sasipreeyajan
The Thai Journal of Veterinary Medicine
In the present study, the efficacy of different vaccination programs against a velogenic Newcastle disease virus (vNDV) challenged in 28-day-old broiler chickens was examined. The chickens were divided into 5 groups, 20 chickens in each. Group 1 did not receive any vaccine and served as the non-vaccinated control group. Groups 2-5 were vaccinated with different vaccination programs against vNDV. At 1-day-old, the chickens in all the vaccinated groups were vaccinated with live Newcastle disease (ND) vaccine, Ulster 2C strain, followed by different inactivated vaccines including vaccine A in groups 2 and 4 and vaccine B in groups 3 and 5. …
Comparison Of Gentamicin Impregnated Polymethlymetacrylate Bead, Gentamicin Coated Native Calcium Sulfate Bead And Gentamicin Coated High Porous Calcium Sulfate Bead On Osteomyelitis Management In A Rat Model, Chaiyakorn Thitiyanaporn, Pareeya Udomkusonsri, Naris Thengchaisri
Comparison Of Gentamicin Impregnated Polymethlymetacrylate Bead, Gentamicin Coated Native Calcium Sulfate Bead And Gentamicin Coated High Porous Calcium Sulfate Bead On Osteomyelitis Management In A Rat Model, Chaiyakorn Thitiyanaporn, Pareeya Udomkusonsri, Naris Thengchaisri
The Thai Journal of Veterinary Medicine
Three types of gentamicin beads were compared in a rat osteomyelitis model. The gentamicin beads were prepared in cylinder shape (diameter 2 mm x height 4 mm). Gentamicin impregnated polymethylmethacrylate (GIPMMA) beads were fabricated according to manufacturer’s instructions. Gentamicin coated native calcium sulfate (GNCS) bead and gentamicin coated high porous calcium sulfate (G-HPCS) bead were prepared in laboratory. Osteomyelitis was induced in the rat’s tibias by using methicillin resistance Staphylococcus aureus (MRSA). After 3 weeks of infection, the infected tibias were implanted with GI-PMMA bead, G-NCS bead, G-HPCS bead or sham treatment (control). Radiographic change, white blood cell count and …
Inactivation Of Infectious Bronchitis Virus With Various Kinds Of Disinfectants, Pheemphat Bengtong, Thotsapol Thomrongsuwannakij, Niwat Chansiripornchai
Inactivation Of Infectious Bronchitis Virus With Various Kinds Of Disinfectants, Pheemphat Bengtong, Thotsapol Thomrongsuwannakij, Niwat Chansiripornchai
The Thai Journal of Veterinary Medicine
This study aimed to perform an in vitro testing of the efficacy of various kinds of disinfectant on infectious bronchitis virus (IBV). Four kinds of disinfectants including Virusnip, Omnicide, CID2000 and Virkon S were used for the virus inactivation test. After challenge with each strain of IBV for each contact time (30 seconds, 1, 5 and 30 minutes), we recorded number of dead embryo/6 inoculated eggs after incubation. Results showed that there was no significant difference among the contact times except 1:800 Virusnip tested for Tha07 (p < 0.05). For Tha03, there was a significant difference among the disinfectants at 30 seconds and 1 minute of contact times (p < 0.05). For Tha08, there was a significant difference among the disinfectants at 1 minute of contact time (p < 0.05). For Tha09, there was a significant difference among the disinfectants at 5 and 30 minutes of contact times (p < 0.05). For Tha10, there was a significant difference among the disinfectants at 5 minutes of contact time (p < 0.05). Virusnip revealed the ability to inactivate the activity of 9 IBV strains in all exposure times, especially at dilutions of 1 : 100 and 1 : 200.
Evaluation Of Hematology Profiles And Measurement Of Serum Cardiac Troponin Level In Canine Monocytic Ehrlichiosis, Rungrote Osathanon, Walasinee Moonarmart, Naiyana Suksantilap, Nattiya Krajangpit, Piyanart Lekcharoensook, Pruksa Julapanthong, Nutthakulporn Wongrerkngam
Evaluation Of Hematology Profiles And Measurement Of Serum Cardiac Troponin Level In Canine Monocytic Ehrlichiosis, Rungrote Osathanon, Walasinee Moonarmart, Naiyana Suksantilap, Nattiya Krajangpit, Piyanart Lekcharoensook, Pruksa Julapanthong, Nutthakulporn Wongrerkngam
The Thai Journal of Veterinary Medicine
Canine monocytic ehrlichiosis (CME) is an important multisystemic disease in dogs which is caused by Ehrlichia canis (E. canis). Systemic inflammatory response syndrome (SIRS) and severe anemia from E. canis might be the potential causes of myocardial damage. Cardiac troponin T (cTnT) is considered to be a sensitive and specific biomarker for myocardial damage in many mammalian species. The objectives of this study were to evaluate the effect of CME on serum cTnT levels in dogs and to determine the relationship between serum cTnT and values obtained from hematology. Fifty-two client-owned dogs were recruited into this study and clinical data …