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The Thai Journal of Veterinary Medicine

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Articles 901 - 930 of 2525

Full-Text Articles in Medicine and Health Sciences

Molecular Characterization Of Pasteurella Multocida Isolates That Cause Haemorrhagic Septicaemia In Thailand Using Pulsed-Field Gel Electrophoresis, Apasara Worarach, Gumtorn Promto, Pornpen Pathanasophon Mar 2014

Molecular Characterization Of Pasteurella Multocida Isolates That Cause Haemorrhagic Septicaemia In Thailand Using Pulsed-Field Gel Electrophoresis, Apasara Worarach, Gumtorn Promto, Pornpen Pathanasophon

The Thai Journal of Veterinary Medicine

Pasteurella multocida causes haemorrhagic septicaemia (HS), which is a severe epidemic disease in cattle and buffaloes. In this study, serological typing, minimum inhibitory concentration (MIC) and pulsed-field gel electrophoresis (PFGE) were used to characterize 87 HS isolates caused by P. multocida from cattle and buffaloes during 1989 to 2011 in comparison with Thai and Laotian vaccine strains and the reference P. multocida strain P1256. The results of serotyping showed that the majority of the isolates (88.5%) belonged to serotype B: 2 (n = 77) while 11.5% shared with serotype B: 2, 5 (n = 10). MIC results showed that 100% …


Perspectives On Antimicrobial Resistance In Livestock And Livestock Products In Asean Countries, Amornthep Archawakulathep, Chung Ta Thi Kim, Dethaloun Meunsene, Didik Handijatno, Hasliza Abu Hassim, Hope R. G. Rovira, Khin Sandar Myint, Loinda R. Baldrias, Meas Sothy, Min Aung, Nenny H. Wahyu, Rortana Chea, Sookruetai Boonmasawai, Soulasack Vannamahaxay, Sunpetch Angkititrakul, Therese M. A. Collantes, Tho Nguyen Van, Veerasak Punyapornwithaya, Zunita Zakaria, Rungtip Chuanchuen Mar 2014

Perspectives On Antimicrobial Resistance In Livestock And Livestock Products In Asean Countries, Amornthep Archawakulathep, Chung Ta Thi Kim, Dethaloun Meunsene, Didik Handijatno, Hasliza Abu Hassim, Hope R. G. Rovira, Khin Sandar Myint, Loinda R. Baldrias, Meas Sothy, Min Aung, Nenny H. Wahyu, Rortana Chea, Sookruetai Boonmasawai, Soulasack Vannamahaxay, Sunpetch Angkititrakul, Therese M. A. Collantes, Tho Nguyen Van, Veerasak Punyapornwithaya, Zunita Zakaria, Rungtip Chuanchuen

The Thai Journal of Veterinary Medicine

Antimicrobial resistance (AMR) in bacteria, particularly in foodborne pathogens, has increasingly become apparent in most parts of the world including ASEAN countries, creating great impact on economy, human and animal health and international food trade. The tendency of bacterial pathogens to become multidrug resistant (MDR) is the most serious concern in AMR. The ASEAN Economic Community (AEC) will be fully established in 2015 and ASEAN cooperation has acknowledged the significance of food safety, of which AMR is one of the major concerns. There is a need for all ASEAN nations to harmonize its AMR monitoring and surveillance programmes in order …


Reintroduction And Behavioral Observations Of Chinese Gorals (Naemorhedus Griseus) In Natural Conditions, Nithidol Buranapim, Narit Sitasuwan, Adisorn Kongprempoon, Keeta Korkusol, Boripat Siriaroonrat, Sumate Kamolnorranath Mar 2014

Reintroduction And Behavioral Observations Of Chinese Gorals (Naemorhedus Griseus) In Natural Conditions, Nithidol Buranapim, Narit Sitasuwan, Adisorn Kongprempoon, Keeta Korkusol, Boripat Siriaroonrat, Sumate Kamolnorranath

The Thai Journal of Veterinary Medicine

The study of the ecology and behavior of gorals (Naemorhedus griseus) was conducted in Maelao-Maesae Wildlife Sanctuary, Chiang Mai Province during July 2012 – July 2013. The behavioral patterns of the gorals were observed using focal-scan sampling and descriptive methods. Results showed that the seven most common behavioral patterns were standing, walking, resting, grooming, scratching, feeding (foraging), and ruminating. Twelve plants were recognized as goral food based on direct field observations. Overall land area used by the gorals in this study was 81,124 m2. The highest number of gorals was found in dipterocarp forests, followed by deciduous forests and the …


Recombinant Human Follicle Stimulating Hormone And Growth Factors Improve The Meiotic And Developmental Competence Of Cat Oocytes, Theerawat Tharasanit, Chommanart Thongkittidilok, Thanida Sananmuang, Mongkol Techakumphu Mar 2014

Recombinant Human Follicle Stimulating Hormone And Growth Factors Improve The Meiotic And Developmental Competence Of Cat Oocytes, Theerawat Tharasanit, Chommanart Thongkittidilok, Thanida Sananmuang, Mongkol Techakumphu

The Thai Journal of Veterinary Medicine

In vitro embryo production has proven as potential tool for understanding factors involving developmental biology of embryos. This study aimed at examining the effects of recombinant human follicle stimulating hormone (rhFSH) on meiotic resumption and also on developmental competence of cat oocytes. Experiment 1 studied mRNA expression of luteinizing hormone (LH), follicle stimulating hormone (FSH), epidermal growth factor (EGF) and insulin like growth factor-1 (IGF-1) receptors (R) in cumulus cells. mRNA expression of LH receptor in cumulus cells after oocyte maturation was additionally examined. Experiment 2 examined the effect of different rhFSH concentrations (0.01, 0.05, 0.1 and 1 IU/ml) on …


Transvenous Pacemaker Implantation In A Schnauzer With Sick Sinus Syndrome, Amornrate Sastravaha, Kriengkrai Jirasirirojanakorn, Jatuporn Noosud, Taksaon Duangurai, Tanhatai Krajarngjang, Wijit Sutthiprapa, Sirirat Niyom, Naris Thengchaisri Mar 2014

Transvenous Pacemaker Implantation In A Schnauzer With Sick Sinus Syndrome, Amornrate Sastravaha, Kriengkrai Jirasirirojanakorn, Jatuporn Noosud, Taksaon Duangurai, Tanhatai Krajarngjang, Wijit Sutthiprapa, Sirirat Niyom, Naris Thengchaisri

The Thai Journal of Veterinary Medicine

A fourteen-year-old female neutered Schnauzer was presented to the hospital with multiple syncopal episodes for two weeks. The dog appeared fatigue and sleepy but remained responsive. Clinical examination revealed bradycardia and irregular pulse. Electrocardiography showed sinus rhythm with repetitively long episodes of sinus arrest over 3 seconds, indicating sick sinus syndrome. The dog eventually underwent a transvenous single chamber permanent pacemaker. She was followed up for a month after surgery without recurrent syncope and irregular heart rate.


Molecular Study Of The Prevalence Of Leptospira Spp. Serovar Hardjo In Blood Samples Of Iranian Cattle And Sheep, Faham Khamesipour, Abbas Doosti, Esmaeil Omrani Mar 2014

Molecular Study Of The Prevalence Of Leptospira Spp. Serovar Hardjo In Blood Samples Of Iranian Cattle And Sheep, Faham Khamesipour, Abbas Doosti, Esmaeil Omrani

The Thai Journal of Veterinary Medicine

Leptospira is an emerging global public health problem which involves both animals and human. The aim of this study was to appraise the presence of Leptospira DNA in blood samples from cattle and sheep via Polymerase Chain Reaction (PCR) method in Chaharmahal VaBakhtiari and Isfahan provinces of Iran. One hundred and ninety five samples (92 blood samples of cattle and 103 blood samples of sheep) were collected randomly from clinical healthy animals. DNA was extracted from the blood samples and stored at -20°C before examination. PCR reaction was performed for detection of Leptospira DNA using specific primers for 16s rRNA …


Recurrent Rumen Tympany Caused By Trichobezoars In Buffaloes (Bubalus Bubalis): A Series Report, Ashraf M. Abu-Seida, Oday S. Al-Abbadi Mar 2014

Recurrent Rumen Tympany Caused By Trichobezoars In Buffaloes (Bubalus Bubalis): A Series Report, Ashraf M. Abu-Seida, Oday S. Al-Abbadi

The Thai Journal of Veterinary Medicine

This series report was carried out for 3 years on 15 buffaloes suffering from recurrent rumen tympany associated with trichobezoars. Full case history, thorough clinical examination, ferroscopy, and vaginal and trans-rectal examination were carried out in all cases. The affected buffaloes were treated with rumenotomy. All cases were lactating buffaloes and had heavy hair coat. The age of affected buffaloes ranged between 5-10 years. Clinical signs included anorexia, depression, sharp decrease in milk yield, intermittent respiratory distress, recurrent rumen tympany and dehydration. Hematological and biochemical analyses revealed leukocytosis, hypokalemia and hypochloremia. Rumenotomy was curative in all cases and revealed trichobezoars …


Well-Differentiated Squamous Cell Carcinoma In A Captive Clouded Leopard (Neofelis Nebulosa), Sawang Kesdangsakonwut, Saowaphang Sanannu, Anudep Rungsipipat, Wijit Banlunar Mar 2014

Well-Differentiated Squamous Cell Carcinoma In A Captive Clouded Leopard (Neofelis Nebulosa), Sawang Kesdangsakonwut, Saowaphang Sanannu, Anudep Rungsipipat, Wijit Banlunar

The Thai Journal of Veterinary Medicine

Squamous cell carcinoma (SCC) is a malignant neoplasm of keratinized epithelial cell affecting both domestic and wild animals. A few cases of SCC have been reported in wild felids. A fifteen year old, intact female, clouded leopard showed signs of lameness and swelling of the right hind paw, which upon physical examination was noted to contain an abscess. Surgical biopsy of the site revealed a well-differentiated SCC. Attempts at surgical excision and cryosurgery proved unsuccessful and there was a recurrence of the mass. Therefore, mid-femoral amputation was performed. Macroscopically, the amputated limb revealed multiple dermal, cavitated whitish masses. Microscopically, the …


Ophthalmology Snapshot, Nalinee Tuntivanich Mar 2014

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.


Ultrasound Diagnosis, Phiwipha Kamonrat Mar 2014

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.


Rapid Single Cell Typing Using Sybr® Green Real-Time Pcr Together With Melt Curve Analysis For Sex Identification Of Porcine Sperm, Varaporn Korchunjit, Kampon Kaeoket, Yindee Kitiyanant, Tuempong Wongtawan Mar 2014

Rapid Single Cell Typing Using Sybr® Green Real-Time Pcr Together With Melt Curve Analysis For Sex Identification Of Porcine Sperm, Varaporn Korchunjit, Kampon Kaeoket, Yindee Kitiyanant, Tuempong Wongtawan

The Thai Journal of Veterinary Medicine

Identification of X or Y chromosome is a very useful technique to verify the sex of boar sperm, but common methods used in pig such as Fluorescence In situ Hybridisation (FISH) and whole semen Polymerase Chain Reaction (PCR) have some limitations. FISH is highly accurate, but time-consuming (>3 days). Whole semen PCR is faster than FISH (3-6 h), but not highly accurate (approximate methods). The objective of this study was to develop a fast and highly accurate protocol to identify sex of boar sperm. In the present study, our team developed an alternative sex identification protocol using single cell …


Clinical Effect Of Glucosamine And Chondroitin Contained Nutraceutical On Osteoarthritis In Dogs After Anterior Cruciate Ligament Rupture Surgical Repair, Prach Maihasap, Kumpanart Soontornwipart, Natee Techaarpornkul Mar 2014

Clinical Effect Of Glucosamine And Chondroitin Contained Nutraceutical On Osteoarthritis In Dogs After Anterior Cruciate Ligament Rupture Surgical Repair, Prach Maihasap, Kumpanart Soontornwipart, Natee Techaarpornkul

The Thai Journal of Veterinary Medicine

Glucosamine and chondroitin sulfates arekey structural components of extracellular fluid in articular cartilage. From previous studies, glucosamine and chondroitin sulfates have several positive effects on cartilage homeostasis and are used in osteoarthritis patients. Although glucosamine and chondroitin sulfates have some positive effects on clinical treatment of osteoarthritis, clinical results still need to be investigated. The objective of this study was to investigate the clinical outcomes on lameness score, radiological score, serum WF6 level and serum HA level of glucosamine and chondroitin sulfates in osteoarthritic dogs, using anterior cruciate ligament rupture patients as study models of osteoarthritis (n=12). The results from …


Isoflurane Concentration When Compared With Intramuscular Morphine During Patellar Luxation Repair In Dogs, Pathompong Thapun, Sumit Durongphongtorn, Marissak Kalpravidh Mar 2014

Isoflurane Concentration When Compared With Intramuscular Morphine During Patellar Luxation Repair In Dogs, Pathompong Thapun, Sumit Durongphongtorn, Marissak Kalpravidh

The Thai Journal of Veterinary Medicine

This clinical trial aimed to assess the intraoperative analgesic effect, based on the required minimum alveolarconcentration (MAC) of isoflurane, of transdermal fentanyl when compared with intramuscular morphine in dogsundergoing patellar luxation repair. Seventeen healthy dogs with grade II or grade III of medial patellar luxationwere randomly assigned to group M or group F, receiving a sham patch or a transdermal fentanyl patch (25 μg/h),respectively, applied on the skin at least 24 h prior to surgery. Three dogs had bilateral medial patellar luxation andwere then enrolled in both groups, resulting in ten animals for each group. Before surgery, group M dogs …


Quantitative Microbial Risk Assessment Of Salmonella In Surface Water As A Source Of Tap Water, Paweeranut Banmairuroy, Phattharaporn Chaichana, Chaiwat Pulsrikarn, Suphachai Nuanualsuwan Mar 2014

Quantitative Microbial Risk Assessment Of Salmonella In Surface Water As A Source Of Tap Water, Paweeranut Banmairuroy, Phattharaporn Chaichana, Chaiwat Pulsrikarn, Suphachai Nuanualsuwan

The Thai Journal of Veterinary Medicine

Salmonella spp. is fecal-orally transmitted and readily spread into the environment. Little is known about risk of utilizing surface water in Thailand. Quantitative microbial risk assessment has been used to estimate the risk of waterborne salmonellosis. For hazard characterization, beta-Poisson model was employed to describe the relationship between dose of Salmonella from water exposure and salmonellosis. Prevalence and concentration of Salmonella in the surface water including water exposure were used to model probability of exposure. The probabilistic prevalence was described by beta distribution and the range of mean Salmonella prevalences of the surface water along rivers were between 8.33% and …


Molecular Characterization Of Respiratory Bacterial Pathogens In Large And Small Ruminants, Gemerlyn Gonzales Garcia, Lawrence Pascual Belotindos, Claro Niegos Mingala Dec 2013

Molecular Characterization Of Respiratory Bacterial Pathogens In Large And Small Ruminants, Gemerlyn Gonzales Garcia, Lawrence Pascual Belotindos, Claro Niegos Mingala

The Thai Journal of Veterinary Medicine

Bacterial isolates from different cases of respiratory infections in small and large ruminants were evaluated using bacterial culture and semi-nested PCR and identification was confirmed by DNA sequencing. Universal primers targeting the bacterial 16S rRNA for bacteria were used. Other sets of primers that were specific for Gram-positive bacteria and combinations of primers specific for Gram-negative organisms were used in the second PCR. The amplified PCR products were subjected to DNA sequence analysis. The DNA sequences of the isolated bacteria were aligned with the DNA sequences of bacteria in the GenBank through BLAST. Results confirmed isolation of three Gram-positive and …


Delayed Post-Ovulatory Progesterone Rise In Thai-Holstein Dairy Heifers: Association With Endocrine Events Around Oestrus, Effects On Conception Rate And Possibility For Progesterone Supplement Post-Insemination, Sudsaijai Kornmatitsuk, Bunlue Kornmatitsuk, Yindee Kitiyanant Dec 2013

Delayed Post-Ovulatory Progesterone Rise In Thai-Holstein Dairy Heifers: Association With Endocrine Events Around Oestrus, Effects On Conception Rate And Possibility For Progesterone Supplement Post-Insemination, Sudsaijai Kornmatitsuk, Bunlue Kornmatitsuk, Yindee Kitiyanant

The Thai Journal of Veterinary Medicine

The current research aimed to further study the delayed post-ovulatory progesterone (P4) rise in Thai-Holstein dairy heifers in the points of an association with endocrine events around oestrus (experiment 1), effects on conception rate and possibility for P4 supplement post-insemination (experiment 2). In experiment 1, the P4 levels in the delayed P4 heifers reached 1 ng/ml later than in the normal ones (6.8±0.4 vs 3.3±1.5 days after oestrus, resp.) (p = 0.053). At ovulation the pre-ovulatory follicles of the delayed P4 were smaller than of the normal P4 heifers (11.8±0.5 vs 13.1±0.2 mm, resp.) (p = 0.013). The levels of …


Comparison Of Nutrient Contents, Digestibility And Fecal Output Of Concentrate Rations (Gel) For Dog, Uttra Jamikorn Dec 2013

Comparison Of Nutrient Contents, Digestibility And Fecal Output Of Concentrate Rations (Gel) For Dog, Uttra Jamikorn

The Thai Journal of Veterinary Medicine

The purposes of this current study were to compare the amount of nutrient contents and digestibility between different concentrate ration brands and their effects on fecal output. These concentrate rations mean the diets formulated in order to improve the nutritive status of the animals. In this study, nine mature female mongrel dogs were randomly subjected into three groups of the triplicated 3x3 Latin square design. Three brands of concentrate ration in form of gel were used as dietary treatments. Nutrient contents (on DM basis), body weight, fecal output, and apparent digestibility of nutrients were determined. Percentages of dry matter, organic …


Pathogenicity And Transmission Of Thai Hpai-H5n1 In Japanese Quails, Thawat Lekdumrongsak, Wijit Banlunara, Rachod Tantilertcharoen, Nutthawan Nonthabenjawan, Komkiew Pinpimai, Jiroj Sasipreeyajan, Alongkorn Amonsin Dec 2013

Pathogenicity And Transmission Of Thai Hpai-H5n1 In Japanese Quails, Thawat Lekdumrongsak, Wijit Banlunara, Rachod Tantilertcharoen, Nutthawan Nonthabenjawan, Komkiew Pinpimai, Jiroj Sasipreeyajan, Alongkorn Amonsin

The Thai Journal of Veterinary Medicine

Pathogenicity and transmission of Highly Pathogenic Avian Influenza subtype H5N1 viruses (HPAI-H5N1) depend on the strains and infection doses of the viruses. In this study, experimental infection was performed to determine pathogenicity and transmission of the Thai HPAI-H5N1 in Japanese quails. One hundred and seven quails were divided into 4 groups, experimental groups 1-3 (n = 32 per group) and negative control group (n = 11). At the age of 9 weeks, 16 quails each from groups 1-3 were inoculated with 107, 105 and 103 EID50 of Thai HPAI-H5N1, A/chicken/Thailand/CUK2/04(CU-K2) virus. Twenty-four hours later, 16 quails were added as contact …


Clastogenic And Anticlastogenic Potential Of Neem Flower Extract Evaluated By Rat Liver Micronucleus Assay, Piengchai Kupradinun, Yaninee Jarratwisarutporn, Rawiwan Wongpoomchai, Hathaitip Sritanaudomchai, Mati Rienkitjakarn Dec 2013

Clastogenic And Anticlastogenic Potential Of Neem Flower Extract Evaluated By Rat Liver Micronucleus Assay, Piengchai Kupradinun, Yaninee Jarratwisarutporn, Rawiwan Wongpoomchai, Hathaitip Sritanaudomchai, Mati Rienkitjakarn

The Thai Journal of Veterinary Medicine

Clastogenic and anticlastogenic effects of neem flower (Azadirachta indica A. Juss) extract were determined using a rat liver micronucleus assay. Methanol extracts of neem flowers (MENF) in 15% Tween 80 at 100 and 500 mg/kg body weight were orally given to male Sprague Dawley rats daily for 6 weeks (2 weeks prior to and 4 weeks during repeated administration of diethylnitrosamine (DEN). All rats were anesthetized and their livers were collected. Hepatocytes were isolated from the livers using a collagenase solution without performing liver perfusion, then stained with 4’, 6-diamidino-2-phenylindole dihydrochloride (DAPI). Incidence of the micronucleated hepatocytes was evaluated by …


An In Vitro Comparative Study Of Aflatoxin B1 Adsorption By Thai Clay And Commercial Toxin Binders, Bundit Tengjaroenkul, Urai Tengjaroenkul, Natapol Pumipuntu3, Komkrich Pimpukdee, Sawitree Wongtangtintan, Piyawat Saipan Dec 2013

An In Vitro Comparative Study Of Aflatoxin B1 Adsorption By Thai Clay And Commercial Toxin Binders, Bundit Tengjaroenkul, Urai Tengjaroenkul, Natapol Pumipuntu3, Komkrich Pimpukdee, Sawitree Wongtangtintan, Piyawat Saipan

The Thai Journal of Veterinary Medicine

Twenty samples of Thai clay from fourteen provinces and seven commercial toxin binders were investigated for their adsorption capacity of aflatoxin B1 (AFB1) in vitro. Each sample of 5 mg/l AFB1 solution was shaken at 250C for 24 hours and supernatants of centrifuged samples were analyzed for concentrations of AFB1 using a UV spectrophotometer. Adsorption capacity was calculated and applied to isothermic equations. The results indicated that Thai clays was capable of sequestering AFB1 from aqueous solution differently and Thai clay from Lopburi and Lamphun provinces had the highest adsorption capacity, similar to commercial binders. S-shaped isothermic curves were observed …


Enhancing Specific Antibodies Using A Developed Sub-Unit Pcv2b Vaccination In A Pcv2-Affacted Herd, Suphattra Jittimanee, Suparlark Nuntawan Na Ayudhya, Roongtham Kedkovid, Komkrich Teankum, Sanipa Suradhat, Roongroje Thanawongnuwech Dec 2013

Enhancing Specific Antibodies Using A Developed Sub-Unit Pcv2b Vaccination In A Pcv2-Affacted Herd, Suphattra Jittimanee, Suparlark Nuntawan Na Ayudhya, Roongtham Kedkovid, Komkrich Teankum, Sanipa Suradhat, Roongroje Thanawongnuwech

The Thai Journal of Veterinary Medicine

Porcine circovirus type 2 (PCV2) causes multisystemic diseases called porcine circovirus associated disease (PCVAD) in pigs leading to a significant economic loss in the swine industry. The objective of this study was to determine the efficacy of a developed sub-unit PCV2b vaccine using a recombinant truncated capsid protein expressed in Escherichia coli in PCVAD-affected pig farms. Five-week-old conventional pigs were allocated into 2 groups: vaccinated (n = 55) and control (n = 55) groups placed in the same building. The vaccinated pigs were given two shots of a developed vaccine with 2-week interval on day 0 and 14. Serum samples …


Ophthalmology Snapshot, Nalinee Tuntivanich Dec 2013

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.


What Is Your Diagnosis, Pranee Tuntivanich, Suwicha Chuthatep, Rampaipat Tungjitpeanpong Dec 2013

What Is Your Diagnosis, Pranee Tuntivanich, Suwicha Chuthatep, Rampaipat Tungjitpeanpong

The Thai Journal of Veterinary Medicine

No abstract provided.


Safety And Toxicity Evaluation Of Bronopol In Striped Catfish (Pangasianodon Hypophthalmus), Patharapol Piamsomboon, Mintra Lukkana, Janenuj Wongtavatchai Dec 2013

Safety And Toxicity Evaluation Of Bronopol In Striped Catfish (Pangasianodon Hypophthalmus), Patharapol Piamsomboon, Mintra Lukkana, Janenuj Wongtavatchai

The Thai Journal of Veterinary Medicine

The study was performed to evaluate the safety and toxicity of bronopol in striped catfish Pangasianodon hypophthalmus. Bronopol product is licensed in Thailand and Vietnam for control of aquatic fungi and ciliated protozoa in scaleless fish at the daily dosage of 10 mg/l, 2-hour static immersion, for 5 consecutive days. Safe concentrations were evaluated in fish exposed to bronopol at the therapeutic dosage (10 mg/l, 2 hours, 5 consecutive days) and 2 and 3 times overdose (20 and 30 mg/l, 2 hours, 5 consecutive days). Fish treated with 10-30 mg/l bronopol for 2 hours daily for 5 consecutive days appeared …


Molecular Characterization Of Chicken Infectious Anemia Virus Outbreaks During 2008-2011 In Thailand, Wisanu Wanasawaeng, Jirayu Buatong, Supawadee Chaichote, Niwat Chansiripornchai Dec 2013

Molecular Characterization Of Chicken Infectious Anemia Virus Outbreaks During 2008-2011 In Thailand, Wisanu Wanasawaeng, Jirayu Buatong, Supawadee Chaichote, Niwat Chansiripornchai

The Thai Journal of Veterinary Medicine

The present study aimed to characterize chicken infectious anemia virus isolated from various outbreaks in Thailand during 2008-2011, and compare them with other strains from the GenBank database using molecular and bioinformatic techniques. For this purpose, thirteen samples were collected from eight commercial farms affected by chicken infectious anemia outbreak. All were positive by PCR and grouped in 2 clusters with phylogenetic analysis based on nucleotide sequencing, consistent with amino acid sequence analysis of the VP1 gene in the hypervariable region. All of the thirteen strains were closely related to Chinese and Japanese strains with a high percentage of sequence …


Testicular Atrophy And Its Related Changes In Culled Boars: A Pathological Investigation, Komkrich Teankum, Padet Tummaruk, Sawang Kesdangsakonwut, Thiti Antarasena, Sithichok Lacharoj, Jinda Singlor, Annop Kunavongkrit, Roongroje Thanawongnuwech Dec 2013

Testicular Atrophy And Its Related Changes In Culled Boars: A Pathological Investigation, Komkrich Teankum, Padet Tummaruk, Sawang Kesdangsakonwut, Thiti Antarasena, Sithichok Lacharoj, Jinda Singlor, Annop Kunavongkrit, Roongroje Thanawongnuwech

The Thai Journal of Veterinary Medicine

Testicular atrophy is a major cause of culling in boars. Pathogenesis of this change is poorly understood. Therefore, we aimed to investigate pathological changes of the genital tracts of culled boars with testicular atrophy. Twenty-eight genital organs of culled boars collected from slaughter houses in Thailand were pathologically examined. According to testicular weight, the organs were allocated into 4 groups: severe atrophy (n = 12), moderate atrophy (n = 4), mild atrophy (n = 4), and normal control (n = 8). In the severe atrophic group, the mean weight of atrophic testes (175.1 ± 51 g) was lower than those …


Determination Of Antimicrobial Susceptibility, Antimicrobial Resistance Genes And In Vivo Testing Of Antimicrobial Susceptibility Of Avibacterium Paragallinarum, Tippayaporn Noonkhokhetkong, Kridda Chukiatsiri, Jiroj Sasipreeyajan, Niwat Chansiripornchai Dec 2013

Determination Of Antimicrobial Susceptibility, Antimicrobial Resistance Genes And In Vivo Testing Of Antimicrobial Susceptibility Of Avibacterium Paragallinarum, Tippayaporn Noonkhokhetkong, Kridda Chukiatsiri, Jiroj Sasipreeyajan, Niwat Chansiripornchai

The Thai Journal of Veterinary Medicine

Avibacterium paragallinarum is the causative agent of infectious coryza, an acute upper respiratory disease in chickens. The aim of this study was to characterize the antimicrobial susceptibility, resistance genes and the in vivo testing of antimicrobial susceptibility in chickens. Eighteen A. paragallinarum isolated in Thailand were tested for antimicrobial susceptibility by broth microdilution method. A high prevalence of resistance MIC pattern to oxytetracycline, doxycycline, streptomycin, ciprofloxacin, erythromycin and sulfamethoxazole-trimethoprim was found. Thirteen isolates showed multiple antimicrobial drug resistance (66.7%). Resistance genes of tet(A), tet(B), tet(M), erm(A), erm(B), blaROB-1 and sul2 were found and the resistance genes of tet(A), tet(B) and …


Efficacy And Safety Of Different Live Mycoplasma Gallisepticum Vaccines In Layer Chickens, Somsak Pakpinyo, Suwarak Wanaratana, Kreangkrai Sangthongdang, Marcelo Paniago Dec 2013

Efficacy And Safety Of Different Live Mycoplasma Gallisepticum Vaccines In Layer Chickens, Somsak Pakpinyo, Suwarak Wanaratana, Kreangkrai Sangthongdang, Marcelo Paniago

The Thai Journal of Veterinary Medicine

The efficacy and safety of different live Mycoplasma gallisepticum (MG) vaccines were determined. One hundred and fifty six-week-old female layer chickens were equally divided into 6 groups. Groups 1-6 received F (two groups), 6/85 and ts-11 strains, positive and negative control, respectively. At 8 weeks old, 5 birds/group were sacrificed and gross thoracic airsac lesion scores (GTALS) and microscopic tracheal lesion scores (MTLS) were blindly evaluated. At 12 weeks old, groups 1-5 were challenged through an eye dropping with approximately 1x106 CFU of MG Thai isolated. At 13 and 14 weeks old, 10 birds/group were swabbed in the palatine fissure …


Screening And Characterization Of Lactic Acid Bacteria From Animal Faeces For Probiotic Properties, Tanatip Thamacharoensuk, Nuttha Thongchul, Malai Taweechotipatr, Vasana Tolieng, Kentaro Kodama, Somboon Tanasupawat Dec 2013

Screening And Characterization Of Lactic Acid Bacteria From Animal Faeces For Probiotic Properties, Tanatip Thamacharoensuk, Nuttha Thongchul, Malai Taweechotipatr, Vasana Tolieng, Kentaro Kodama, Somboon Tanasupawat

The Thai Journal of Veterinary Medicine

Twenty-two bacterial isolates from wild elephant faeces and 4 isolates from buffalo faeces in Thailand were investigated for probiotic properties and their lactic acid production. On the basis of their phenotypic characteristic clustering and 16S rRNA gene sequence analysis, the isolates were divided into seven groups. Group I (10 isolates) was identified as Enterococcus hirae, group II (4 isolates) as E. avium, group III (3 isolates) as Pediococcus pentosaceus , IV (3 isoaltes) as P. acidilactici, group V (3 isolates) as Lactobacillus pentosus, group VI (1 isolate) as Lactococcus garvieae and group VII (2 isolates) as Weissella paramesenteroides. All of …


Milk Production And Mammary Extraction Of Nutrients By Late Lactating Cross-Bred Saanen Goats Supplemented With Betaine In Diet, Nungnuch Saipin, Somchai Chanpongsang, Narongsak Chaiyabutr Dec 2013

Milk Production And Mammary Extraction Of Nutrients By Late Lactating Cross-Bred Saanen Goats Supplemented With Betaine In Diet, Nungnuch Saipin, Somchai Chanpongsang, Narongsak Chaiyabutr

The Thai Journal of Veterinary Medicine

The objective of this study was to investigate the effect of supplementary betaine in diet on milk secretion, milk composition, mammary extraction of nutrients and relevant parameters in late lactating cross-bred Saanen goats. Ten, multiparous, non-pregnant cross-bred Saanen goats of approximately 10 weeks postpartum were used for the study. The animals were divided into two groups of five animals each. Animals in the experimental group received diet containing 4 g betaine per kg of concentrate diet for four weeks, while the control group received similar concentrate diet without betaine as concurrent control. Results showed that milk yields of betaine supplemented …