Medicine and Health Sciences Commons^™

Neutrophil-To-Lymphocyte, Monocyte-To-Lymphocyte, And Platelet-To-Lymphocyte Ratio In Acute Hospitalized Patients With Schizophrenia, Apichan Dangrungroj, Mayteewat Chiddaycha

Chulalongkorn Medical Journal

Background: Schizophrenia has been increasingly linked to inflammation. The neutrophil-to-lymphocyte ratio (NLR), monocyte-to-lymphocyte ratio (MLR), and platelet-to-lymphocyte ratio (PLR) have emerged as potential indicators of systemic inflammation.

Objectives: The objectives are to compare NLR, MLR, and PLR between patients with schizophrenia and healthy controls, and to investigate the factors associated with NLR. Methods: This retrospective study included 102 sex- and age-matched patients hospitalized with schizophrenia and control subjects. NLR, MLR, and PLR were calculated from the Complete Blood Count (CBC) results and compared between groups. Sociodemographic data and clinical characteristics were analyzed using multiple linear regression to identify …

Comparison Of Blood And Urinary Ketamine Profiles Among Different Causes Of Death In Thai Postmortem Cases, Supanida Leungsukjaroen, Sunitda Khawthong, Peerayuht Phuangphung

Chulalongkorn Medical Journal

Background: Ketamine is a substance that is commonly abused, especially in young adults. Blood and urinary ketamine profiles are important for the interpretation of causes of death. Objective: To compare blood and urinary ketamine profiles between different causes of death in Thai postmortem cases.

Methods: A cross-sectional study was conducted in 40 Thai postmortem cases whose urine samples tested positive for ketamine. Sex, age, cause of death, manner of death, blood alcohol concentration (BAC), and concomitant drugs found with ketamine in subjects were recorded for each case. Blood and urinary ketamine and its two metabolites (norketamine and dehydronorketamine …

The Comparison Of Lung Shunt Fraction Calculation Using Tc-99m Maa Planar And Spect/Ct Imaging For Y-90 Radioembolization, Nut Noipinit, Thunyaluk Sawatnatee, Benchamat Phromphao, Peangtawan Ratsameechot, Sutida Thiambandit, Tanawat Sontrapornpol

Chulalongkorn Medical Journal

Background: The evaluation of hepatopulmonary blood vessels with Technetium-99m macroaggregated albumin (Tc-99m MAA) scintigraphy before radioembolization with Yttrium-90 (Y-90) microspheres is a test to assess and prevent radiation pneumonitis. It was determined by the lung shunt fraction (LSF) which was calculated from a planar and SPECT/CT images. This study aimed to compare of LSF calculation from Tc-99m MAA planar and SPECT/CT images for Y-90 radioembolization treatment of liver tumors. Methods: A total of 64 patients (42 men and 22 women, mean age 64 ± 12.5 years) who underwent Tc-99m MAA scanning between 2015 and 2022 were enrolled. LSF was calculated …

Prevalence Of Carbapenem-Resistant Klebsiella Pneumoniae In King Chulalongkorn Memorial Hospital, 2019-2023, Napawan Punakabutra, Thanittha Wongkejkit, Nopphon Wuthinantiwong, Tanittha Chatsuwan

Chulalongkorn Medical Journal

Background : Carbapenem-resistant Klebsiella pneumoniae(CR-KP) poses a significant threat to global public health, contributing to high rates of morbidity and mortality.

Objective : The aim of this study was to investigate the prevalence of CR-KP isolated at King Chulalongkorn Memorial Hospital from April 2019 to December 2023, focusing on its distribution across varoius hospital units, including in-patient (IPD), out-patient (OPD), emergency (ER), and intensive care units(ICU).

Methods : A total of 8,983 K. pneumoniae isolates from patients aged > 15 years at King Chulalongkorn Memorial Hospital between April 2019 and December 2023 were studied. The antimicrobial susceptibility to carbapenems was …

Dual Role Of Triphala Extracts In Breast Cancer Therapy: Enhanced Paclitaxel Cytotoxicity By Ethanolic Extract And Cytoprotection By Aqueous Extract, Supamas Charucharana, Poonlarp Cheepsunthorn Ph.D., Chalisa L. Cheepsunthorn Ph.D.

Chulalongkorn Medical Journal

Background: Combining antioxidants with chemotherapy is an emerging strategy to reduce chemotoxicity in normal cells. Triphala (TPL), a polyphenol-rich Thai herbal formulation, has shown promise in enhancing chemotherapy efficacy, but its interaction with paclitaxel (PX) in breast cancer treatment remains underexplored.

Objective: This study evaluates the effects of aqueous (TPL-w) and ethanolic (TPL-a) TPL extracts combined with low-dose PX on breast cancer cells and non-tumorigenic mammary epithelial cells.

Methods: The bioactive components of TPL-w and TPL-a were identified using high-performance liquid chromatography (HPLC). Thiazolyl blue tetrazolium bromide (MTT) and chloromethyl 2′,7′-dichlorodihydrofluorescein diacetate (CM-H₂DCFDA) assays assessed cell viability and …

Dengue At Photharam Hospital: One Among Ten Clinical Trial Sites Of The World's First Dengue Vaccine, Anongrat Tiawilai, Chawin Tiawilai, Thawat Tiawilai, Nattachai Srisawat, Umaporn Limothai, Terapong Tantawichien, Usa Thisyakorn

Chulalongkorn Medical Journal

Background: Dengue, a viral disease transmitted by vectors, is widespread worldwide, impacting more than 120 nations. In 2019, a concerning 5.2 million dengue cases were reported, with Asia bearing 70% of the global burden. Half of the global population faces the risk of dengue.

Objectives: The goal of this research was to illustrate the epidemiological pattern of dengue patients admitted to Photharam Hospital in Ratchaburi province, Thailand, one of the ten clinical trial sites for the world's first dengue vaccine.

Methods: Data from dengue patients admitted to Photharam Hospital, a provincial healthcare center in Ratchaburi province, Thailand, covering the period …

Incidence Of Cytogenetic Abnormalities Detected By Fish Analysis In Multiple Myeloma Cases: A Seven-Year Study From A Cytogenetic Laboratory In King Chulalongkorn Memorial Hospital, Thailand (2018–2024), Montakarn Tansatit, Nutcharee Jongpornchai, Suwannee Songchart, Kittipornpan Krajokpap, Hudadini Da-Oh

Chulalongkorn Medical Journal

Background: Multiple myeloma (MM) is a genetically heterogeneous plasma cell malignancy with cytogenetic abnormalities influencing prognosis and treatment outcomes. Fluorescence in situ hybridization (FISH) is crucial for detecting clinically significant abnormalities, including IGH translocations and deletions (e.g., del(17p)), particularly in non-dividing plasma cells. However, cost and accessibility challenges limit comprehensive testing in resource-constrained settings like Thailand. Objectives: To investigate the incidence of cytogenetic abnormalities detected by FISH in MM cases over seven years in a Thai population, highlighting regional trends and barriers to comprehensive testing. Methods: A retrospective analysis was conducted on 360 bone marrow samples from MM patients between …

Short-Term Outcomes Of A Comprehensive Rehabiliation Program With Peripheral Magnetic Stimulation In Polyneuropathy: A Case Report, Tuangtip Surarangsit

Chulalongkorn Medical Journal

Polyneuropathy, a condition affecting the peripheral nervous system, often results in sensory and motor dysfunction in older adults. Treatment options include medications and various physical therapy modalities including Peripheral Magnetic Stimulation (PMS). However, evidence supported treating nerve dysfunction with PMS is limited. In this case, an 84-year-old male with a history of diabetes mellitus and osteoarthritis presented with bilateral lower extremity sensory and motor polyneuropathy, experiencing weakness, sensory deficits, and gait instability. After limited success with conventional treatments, a comprehensive rehabilitation program was implemented over four weeks, integrating PMS, occupational therapy, and advanced gait training. Following the intervention, light touch …

A Tubo-Ovarian Abscess In Aplastic Anemia Presenting As Fever Of Unknown Origin, Rashi Mittal, Saurabh Kumar Gautam, Prasan Kumar Panda, Latika Chawla

Chulalongkorn Medical Journal

This report describes a complex case of a tubo-ovarian abscess (TOA) presenting atypically as fever of unknown origin (FUO) in a young woman initially diagnosed with severe aplastic anemia. The case involved protracted febrile illness, heavy menstrual bleeding, and pancytopenia. Identification of an Escherichia coli tubo-ovarian abscess, rather than tuberculosis, emphasized the need for a thorough evaluation in atypical presentations of TOA. Negative tuberculosis tests highlighted the variety of possible microbial etiologies. The resolution of fever following drainage and tailored antibiotic therapy, along with timely surgical intervention, demonstrated the importance of specific management strategies in such cases. Awareness of the …

Combined Anterior Palatoplasty With Barbed Suspension Pharyngoplasty In The Treatment Of Obstructive Sleep Apnea, Patheemon Thanachaikun, Natamon Charakorn

Chulalongkorn Medical Journal

Background: Surgery is a treatment option for obstructive sleep apnea (OSA) patients for whom positive airway pressure therapy is not chosen. Various palatal surgical techniques have been developed. However, the medical literature has limited accounts concerning the use of combination of these techniques to improved outcomes.

Objective: The aim of this study is to assess the efficacy of a combined anterior palatoplasty (AP) and barbed suspension pharyngoplasty (BSP) in treating adult OSA.

Methods: Data of OSA patients who underwent the combined AP with BSP between July 2020 to May 2024 were retrospectively reviewed. The surgical outcomes, including apnea-hypopnea index (AHI), …

The Factors Affecting The Distribution Of Scorpions (Arachnida: Scorpions) In Iran, Hassan Nasirian

Chulalongkorn Medical Journal

Background: The distribution of scorpions is influenced by various factors that determine their presence and abundance in different regions. Understanding these factors is crucial for comprehending scorpion ecology and predicting their potential impact on ecosystems. Objective: These subtopics aim to provide an overview of the distribution of scorpions within Iran and the factors affecting their distribution, highlighting their ecological preferences and adaptations to different regions.

Methods: Forty-nine documents were collected from well-known indexing services, including Elsevier, Google Scholar, PubMed, ScienceDirect, Scopus, Springer, and Web of Science. After carefully studying the documents, they were classified into specific topics and discussed.

Results: …

Job Strain And Associated Factors Among Medical School Employees In Thailand: A Cross-Sectional Study, Jakarin Apisuttisin, Pornchai Sithisarankul, Jate Ratanachina

Chulalongkorn Medical Journal

Introduction: Mental health issues are rising among workers, often linked to job strain. Medical schools, with their diverse workforce including not only healthcare workers and educators but also support staff, are in demanding work environments and are susceptible to job strain. This study aimed to investigate the prevalence and associated factors of job strain among support staff at a medical school in Bangkok, Thailand.

Methods: A cross-sectional study of 721 support staff employed at a medical school in Bangkok, Thailand was conducted between May and July 2024. The questionnaire consisted of three sections including demographic data, work-related factors, and the …

Clinical Factors Predicting The Need For Biopsy In Children With Cervical Lymphadenopathy, Pornchai Achatsachat, Nimmita Srisan, Prapapan Rajatapiti, Katawaetee Decharun, Somboon Reukvibunsi, Paisarn Vejchapipat

Chulalongkorn Medical Journal

Background: Lymphadenopathy in children is often a diagnostic challenge for clinicians. It may be one symptom of many diseases including granulomatous diseases and malignancy. The cervical region is a commonly involved area in peripheral lymphadenopathy. Surgical biopsy often provides a definitive diagnosis but occasionally unnecessary in some cases.

Objectives: The objective was to describe children with cervical lymphadenopathy who underwent surgical biopsies and to determine clinical factors that can predict the likelihood of lymph nodes needing further management (LNFM) including granulomatous and malignant lymph nodes. Methods: Data of 87 children with cervical lymphadenopathy undergoing surgical biopsy were retrospectively reviewed. Descriptive …

The Advanced Strategies Of Human And Donor Milk Fortification And Its Effect On Long-Term Outcome In Preterm Babies, Ashwini Lakra, Mahaveer Singh Lakra, Ishani Arora, Heena Bhandekar, Amar Taksande, Roshan Prasad, Mayur Wanjari

Chulalongkorn Medical Journal

Poor growth in intrauterine and extrauterine life and inadequate protein supplementation in babies during the first weeks of premature babies are linked to long-term growth failure and poor brain development. The calorie requirements of premature newborns cannot always be fulfilled, even with routine and traditional fortification. This is because traditional fortification procedures rely on the presumed well-known composition of human milk rather than the calculated one. The protein content of mothers' milk of preterm babies varies, decreases with breastfeeding duration, and differs with donor human milk being preserved in milk banks for a long duration. Newer fortification techniques are needed …

Appropriate International Normalized Ratio Follow-Up Interval In Patients With Atrial Fibrillation Taking Warfarin, Komsing Methavigul, Jirayu Sutasanasuang

Chulalongkorn Medical Journal

Background: The international normalized ratio (INR) is recommended to be followed up every four-week intervals in patients with atrial fibrillation (AF) taking warfarin. However, data about the appropriate INR follow-up interval are lacking in those patients.

Objective: We aimed to investigate the appropriate INR follow-up intervals in AF patients receiving warfarin between 6-week and 12-week intervals of follow-up appointments.

Methods: We retrospectively enrolled patients with AF taking warfarin at the Central Chest Institute of Thailand between January 2017 to May 2023. The primary outcome was time in therapeutic range (TTR) in each group of follow-up interval and secondary outcome …

Antioxidant And Chemotherapeutic Synergy: Triphala Enhances Doxorubicin Cytotoxicity In Breast Cancer Cells And Reduces Toxicity In Non-Tumorigenic Cells, Supamas Charucharana, Poonlarp Cheepsunthorn Ph.D., Chalisa Louicharoen Cheepsunthorn Ph.D.

Chulalongkorn Medical Journal

Background: The combining of antioxidants with chemotherapy is increasingly explored to minimize toxicity in non-cancerous cells during breast cancer treatment. Triphala (TPL), a Thai herbal compound rich in antioxidants, shows potential as a complementary candidate for breast cancer chemotherapy.

Objective: This study investigated the combined effects of TPL and low-dose doxorubicin (DOX) on human breast cancer and non-tumorigenic mammary epithelial cells.

Methods: TPL’s bioactive compounds were analyzed via high-performance liquid chromatography (HPLC). Cell viability and reactive oxygen species (ROS) levels were assessed in breast cancer (MDA-MB-231, MCF-7) and epithelial (MCF-10A) cells using thiazolyl blue tetrazolium bromide (MTT) and chloromethyl 2′,7′-dichlorodihydrofluorescein …

Blood Tnf-Α And Gabra-1 Levels In Individuals With Poor Ovarian Response Underwent In Vitro Fertilization Program, Gadsara Ruangprachum, Paweena Thuwanut, Wisan Sereepapong, Vorasuk Shotelersuk, Siraprapa Tongkobpetch, Porntip Sirayapiwat

Chulalongkorn Medical Journal

Background: Poor ovarian response (POR) to ovarian stimulation during in vitro fertilization (IVF) program remains a critical issue contributing to decreased ovarian reserve and cancellation of IVF cycles. Nevertheless, the complete etiology of PRO condition remains inadequately understood.

Objectives: This research primarily aimed to compare levels of blood pro-inflammatory cytokine (TNF-α) and apoptotic marker (GABRA-1) between those exhibiting normal ovarian response (NOR) and those with poor ovarian response during IVF program.

Methods: A cross-sectional study involving whole blood collection from 25 participants was conducted (15 individuals in NOR group and 10 in POR group). Relative gene expression levels of blood …

Reliability And Validity Of Thai Version Genital Appearance Satisfaction (Gas) Questionnaire, Suvit Bunyavejchevin, Thanawat Sangnucktham, Purim Ruanphoo

Chulalongkorn Medical Journal

Background: GAS questionnaire is constructed to measure feelings towards women’ own genitals in a broader spectrum. Thai language GAS questionnaire will be useful for clinical and research purpose.

Objectives: The aim of this study was study the reliability and validate the Thai version GAS questionnaire.

Materials and methods: Ninety Thai women attending the gynecologic outpatient clinic at King Chulalongkorn Memorial hospital were recruited between July 2017 to June 2018 in this study. Ten participants dropped out because they did not answer the second questionnaire. The original English-version of GAS questionnaire was translated into Thai version and backward translated …

Quality Control Of Clinical Protocols Of Ct Using The Accreditation Phantom, Walaiporn Suksancharoen, Apawadee Chakrapong, Sakultala Ruenjit, Anchali Krisanachinda

Chulalongkorn Medical Journal

Background: The daily quality control of the Computed Tomography system consists of the measurement of the accuracy of CT Number and the artifact evaluation. The annual quality control includes the clinical protocol review. The CT radiologist is responsible for ensuring that all Quality Assurance (QA) requirements are met. The clinical team reviews and manages the CT protocol to deliver the appropriate radiation dose to the patient for each examination.

Objectives: To verify CT Number Accuracy, to review clinical protocols and to verify that the low-contrast performance of clinical protocols is adequate for diagnosis.

Methods: American College of Radiology (ACR) CT …

Exploring The Therapeutic Potential Of Trigonella Foenum-Graecum Extract-Loaded Transferosomal Gel In Freund's Complete Adjuvant-Induced Rat Models., Vandana Singh Ms., Hema Arya Ms., Bhavana Singh Dr., Koushal Dhamija Mr., Preeti Singh Dr., Gautam Kumar Dr.

Chulalongkorn Medical Journal

Abstract:

Background: Rheumatoid arthritis (RA) is a debilitating autoimmune disorder characterized by inflammation and joint damage.

Objective: This study aimed to evaluate the antiarthritic activity of Trigonella foenum-graecum (TFG) Transferosomal gel in a Freund’s adjuvant-induced arthritic rat model and compare it with Diclofenac gel, a standard anti-inflammatory drug.

Methods: Arthritis was induced in rats by injecting a 0.1 mL (1mg/ml) CFA suspension into the left hind foot in the subplantar region, allowing them to develop arthritis for 21 days.

Results: Arthritis induction led to a significant increase in paw volume, indicative of inflammation. Treatment with both TFG-transferosomal gel and Diclofenac …

Dengue Cases In Hanoi, Vietnam: Unseasonal Surge Foreshadows Increased Incidence That Demands Organized Preventive Action, Andrew W. Taylor-Robinson

Chulalongkorn Medical Journal

Letter to the Editor - Abstract not required.

The Giant Omphalocele Contenting A Surprised Congenital Segmental Intestinal Dilatation, A Case Report And Review Literature., Sirima Liukitithara

Chulalongkorn Medical Journal

This report described the characteristics and management of a rare condition, a segmental dilated small intestine, which has a mimic-liver appearance in giant omphalocele. After the dilated intestine was resected and end-to-end anastomosis was performed, the abdomen could be closed simultaneously without immediate complications. We reviewed the literature on congenital segmental intestinal dilatation. Although the nature of this condition is still unknown, all reports showed the same excellent operative outcome. Finally, awareness of congenital segmental intestinal dilatation in giant omphalocele leads to proper management.

Treatment Gaps In Preventive Treatment-Eligible Migraine Patients: A Hospital-Based Study In Thailand, Thanin Asawavichienjinda

Chulalongkorn Medical Journal

Background: Preventive treatment of migraine is necessary for patients with frequent migraine attacks to avoid migraine progression and medication overuse. In the literature, there have been a few studies, and all of them have demonstrated the low prevalence of preventive-eligible migraine patients having received migraine preventive treatments.

Objective: This study aimed to investigate the prevalence of using preventive migraine treatments in treatment-eligible migraine patients in Thailand[HI1] .

Methods: A hospital registry-based cross-sectional study was conducted in migraine patients between January 2007 and June 2022 at the Chulalongkorn Comprehensive Headache Center, King Chulalongkorn Memorial Hospital, Thailand. Data collected at the …

Association Of Socio-Demographic Profile And The Underlying Risk Factors Of Hypertension Among The Locals Of Prosperidad, Agusan Del Sur, Philippines, Florence Jhun F. Almadin

Chulalongkorn Medical Journal

Background: Hypertension is a leading risk factor for cardiovascular diseases, including heart disease and stroke, which are significant causes of morbidity and mortality worldwide. Its prevalence varies across regions and is influenced by genetic, environmental, and lifestyle factors.

Objectives: This study aimed to determine the socio-demographic profile, the prevalence of hypertension, and the risk factors and associate these with the prevalence of hypertension in Prosperidad, Agusan del Sur.

Methods: Data was gathered via a survey of respondents aged 25-75 from November 2022. Multivariate logistic regression was used to determine the association between the sociodemographic profile and the risk factors for …

Relationship Between Parathyroid Hormone Level And Parathyroid Gland Size And Numbers In Renal Hyperparathyroidism, Thanattha Intrarak, Netsiri Damrongpisutikul, Tunchanok Paprad

Chulalongkorn Medical Journal

Background: Serum parathyroid hormone (PTH) is the gold standard for the diagnosis of renal hyperparathyroidism. Persistent hyperparathyroidism in chronic kidney disease patients results in hyperplasia of the parathyroid glands. Therefore, parathyroid gland size may be an alternative marker to assess the severity of hyperparathyroidism.

Objective: The aim of our study is to investigate the relationship between serum PTH levels and the size and number of parathyroid glands in patients with renal hyperparathyroidism.

Methods: We retrospectively identified patients with renal hyperparathyroidism who underwent focused ultrasound of the parathyroid glands at our institution between August 1st, 2010, and June 30th, 2019. The …

Reliability And Validation Of Thai Version Of Cosmetic Procedure Screening (Cops) Questionnaire In Thai Women, Suvit Bunyavejchevin, Thanawat Sangnucktham, Purim Ruanphoo

Chulalongkorn Medical Journal

Background: Body dysmorphic syndrome is the psychiatric disorder that can be dissatisfied with the cosmetic surgery outcome and disapproval.

Objectives: The aim of this study was to study the reliability and validate the Thai version COPS questionnaire.

Materials and methods: Fifty six Thai women attending the gynecologic outpatient clinic at King Chulalongkorn Memorial hospital were recruited between July 2017 to June 2018 in this study. Four participants dropped out because they did not answer the second questionnaire. The original English-version of COPS was translated into Thai version and backward translated by another linguist. Then the content of questionnaire …

Comparison Of Efficacy Between Thai-Made Replacement Parts (Smart Litho Plus) And Standard Electromagnetic Shock Wave Systems For Renal Stone Treatment, Supoj Ratchanon, Patranuch Noppakulsati

Chulalongkorn Medical Journal

Abstract

Background: Urinary stones are one of the most common urological problems in Thailand. Extracorporeal shock wave lithotripsy (ESWL) is one of the most frequently used treatments due to its noninvasive nature and widespread availability. However, the effectiveness of lithotripters with power sources produced in Thailand (Smart Litho Plus) compared with that of standard machines is poorly established. Therefore, we aim to compare the efficacy of Thai-made replacement parts with that of standard electromagnetic shock wave systems for the treatment of renal stones.

Methods: We conducted a multicenter retrospective cohort study involving 121 patients with renal stones across three sites …

Effects Of Progressive Neuromuscular Control Training On Functional Mobility In Stroke With Bilateral Oa Knee, Sachi Rajeev Jain, Sandeep Shinde "Dr.", Shamika Shamsundar Baraskar

Chulalongkorn Medical Journal

Introduction- A cerebrovascular accident or stroke is the fourth major cause of mortality and the fifth major condition leading to disability in India [1]. Musculoskeletal disabilities such as osteoarthritis limit functional mobility and interfere with rehabilitation. While conventional exercises do not target improving neuromuscular or sensorimotor function, research on neuromuscular exercises in patients with degenerative pathology of the knee has been done; however, research on the effects of these exercises in stroke survivors with osteoarthritis of the both knees have not been done.

Method- 100 participants, aged 45 to 75, were distributed equally for this case control study: …

Proactive Use Of Perturbation Training On Balance And Posture Control In Geriatric Rehabilitation- An Experimental Study, Reema Joshi, Kinjal Shah

Chulalongkorn Medical Journal

Background:

Ageing is a complex process involving a range of variables that interact to cause deterioration of various physiological functions.

Objectives:

Study attempts to find effect of Perturbation training on postural control upon Centre of Gravity (COG) sway velocity in older individuals using modified-Clinical Test of Sensory Integration on Balance (mCTSIB) component on NeuroCom Balance master® and Timed up and go(TUG) test.

Methods:

An experimental study, employing a pre-post intervention model, involved 30 individuals aged 65-85 years with mild to moderate fall risk was conducted at Tertiary Hospital, Pune from August 2022 to January 2023. Perturbation Training was given …

Phytochemical Profiling And Antioxidant Potential Of Methanol Extract Of Vitis Vinifera L Seeds., Sindhuja Lakshmi, Kesavaram Padmavathy Dr, Thirunavukarasu Umaarasu Dr, Dhandapani Prabu Dr

Chulalongkorn Medical Journal

Background: Grapes are the most widely consumed fruit around the world. Grape seed comprises a group of polyphenols are known to have a broad pharmacological and therapeutic activities. The grape seed extract was known to have counteract against oxidative stress by its free radical scavenging activity.

Objectives: To identify the major phytocompounds of grape seed methanol extract and to evaluate its antioxidant activity

Methods: In this study, the methanol extract of the grape seed was subjected to Gas Chromatography-Mass Spectrometry to identify the major phytochemical compounds and further antioxidant activity was also evaluated by DPPH method using AOAC (Association of …