Medicine and Health Sciences Commons^™

Detection Of Genetic Variations Using Rapd Markers In Siberian Huskies Affected With Swimming Puppy Syndrome, Siriwadee Chomdej, Arphaphorn Dokphut, Waranee Pradit, Korakot Nganvongpanit

The Thai Journal of Veterinary Medicine

Swimming puppy syndrome (SPS) is a curious disease found in dogs. The causes and pathology of this disease are still unknown, although heredity is usually considered to be one of the underlying factors. The objective of this study was to investigate the association between genetics and SPS in a Siberian husky model. Four Siberian husky puppies diagnosed with SPS were subjects of this study, while three other healthy Siberian husky puppies served as controls. Blood samples were collected for DNA isolation, using random amplified polymorphic DNA (RAPD) technique with 16 random primers. No genetic variation was found between affected puppies …

Chemical Immobilization Of Bornean Leopard Cats (Prionailurus Bengalensis Borneoensis) With Tiletamine And Zolazepam Under Field Conditions In Borneo, Nájera Fernando, Cediel-Algovia Rafael, Hearn Andrew, Ross Jo, Dench Rosalie, Alcázar Paloma, Nathan Senthivel, Iñaki De Gaspar, Revuelta Luis

The Thai Journal of Veterinary Medicine

Nine wild Bornean leopard cats were anesthetized using a combination of tiletamine and zolazepam (Zoletil©) after being captured in humanely-designed live traps in Sabah, Malaysian Borneo, for the purpose of fitting radio-collars. For five leopard cats (group 1) a single dose of 6.92±1.06 mg/kg of Zoletil© was administered. The mean induction time from the initial Zoletil© dose was 7.9 ± 1.77 minutes, and the mean anesthesia time was 47.2 ± 25.1. For 4 leopard cats (group 2) after an initial mean dose of 6.92±1.06 mg/kg of Zoletil©, it was necessary to administer a second dose (or booster) of Zoletil© (mean …

Investigation Into Bacillus Anthracis Spore In Soil And Analysis Of Environmental Parameters Related To Repeated Anthrax Outbreak In Sirajganj, Bangladesh, Md. Murshidul Ahsan, Mohammad Ferdousur Rahman Khan, Md. Bahanur Rahman, Shah Md. Ziqrul Haq Chowdhury, Md. Shafiullah Parvej, Mueena Jahan, K. H. M. Nazmul Hussain Nazir

The Thai Journal of Veterinary Medicine

The study was conducted for the isolation and detection of Bacillus anthracis spores from soil collected from Sirajganj district (a north-western district of Bangladesh), and to assess the parameters that may relate to the repeated anthrax outbreak. A total of 48 soil samples were collected from the study area during January to November 2012. Endospores were extracted from soil and the Bacillus anthracis was identified using conventional bacteriological, biochemical and sensitivity test against Penicillin-G. The viable B. anthracis spores could be detected from 14 (29.17%) soil samples. Moisture content, pH, calcium and organic carbon contents of the soils were measured …

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

Acute Pulmonary Toxicity Caused By Single Intratracheal Instillation Of Various Doses Of Colloidal Silver Nanoparticles In Mice: Pathological Changes, Particle Bioaccumulation And Metallothionien Protein Expression, Theerayuth Kaewamatawong, Wijit Banlunara, Pattwat Maneewattanapinyo, Chuchaat Thammacharoen, Sanong Ekgasit

The Thai Journal of Veterinary Medicine

To study acute lung toxicity of various doses of colloidal silver nanoparticles (Ag-NPs), mice were intratracheally instilled with 0, 10, 100, 1000 or 10,000 ppm of Ag-NPs. Histopathology, autometallography (AMG) and immunohistochemistry were determined at 1, 3, 7 and 15 days post-exposure.Instillation of 100, 1,000 and 10,000 ppm Ag-NPs produced moderate to severe necrotizing bronchitis and alveolitiswith hypertrophy and hyperplasia of alveolar epithelial cells. The severity of the pulmonary inflammation and damage increased in a dose-dependent manner. Concomitant lamininimmunohistochemical findings generally correlated with pulmonary lesions. Interleukin 1- beta (IL-1β) and tumor necrotic factor-alpha (TNF-α) positive immunostaining were found in the …

Development Of Enzyme-Linked Immunosorbent Assay To Detect Antibodies Against Chicken Infectious Anemia Virus, Niwat Chansiripornchai, Sangkae Pongthanes, Piyarat Chansiripornchai, Wisanu Wanasawaeng

The Thai Journal of Veterinary Medicine

standardized and robust indirect enzyme-linked immunosorbent assay (ELISA) to detect antibodies against chicken infectious anemia virus (CIAV) was developed. Thai CIAV was isolated and used for the entirety of this study. For virus cultivation, a cell density of MDCC-MSB1 cells at 3 x 106 cells/ml and a fetal bovine serum concentration at 10 percent were the most appropriate for Thai CIAV. Then, crude virus of CIAV was prepared using ultracentrifugation technique. To develop the ELISA test, optimal dilution of goat anti-chicken IgG was 1 : 200 and antigen concentration in the ELISA was 1.00 μg/ml, as determined by checkerboard titration. …

What Is Your Diagnosis, Pranee Tuntivanich, Suwicha Chuthatep1

The Thai Journal of Veterinary Medicine

No abstract provided.

Bone Cell Function: A Review, Hoai Nam Nguyen, Naruepon Kampa

The Thai Journal of Veterinary Medicine

Bone is dynamic tissue which is continuously formed and absorbed by its own cells in response to stimuli such as hormones, mechanical loading and growth factors. Understanding the function of bone cells is important, not only in orthopedic field but also in research study involving bone. Bone cells work in harmony to maintain a balance between bone formation and resorption, ultimately to control bone structure and function. Osteoblasts are cells, which contribute to deposition of organic components of bone extracellular matrix. They control recruitment, differentiation and maturation of osteoclasts that participate in resorption activity. In addition, osteoclasts associated with bone …

Short Term Echocardiographic And Clinical Effects Of Ramipril On Dogs With Asymptomatic Degenerative Mitral Valve Disease, Prakit Kohkayasit, Sirilak Surachetpong

The Thai Journal of Veterinary Medicine

Angiotensin converting enzyme (ACE) inhibitors have beneficial effects on degenerative mitral valve disease (DMVD) dogs with stages C and D (ACVIM classification) and on dogs with congestive heart failure. However, ACE inhibitors’ effects on stage B2 or asymptomatic DMVD dogs have still been uncertain. Ramipril is an ACE inhibitor that has lipophilic effects and can suppress ACE in cardiac tissue effectively. We hypothesized that ramipril had beneficial effects on dogs with naturally occurring DMVD in stage B2. Twenty dogs with stage B2 DMVD, weighing between 3-12 kg and being older than 6 years, were recruited into the study. The dogs …

Effects Of Environmental Factors, Ages And Breeds On Semen Characteristics In Thai Indigenous Chickens: A One-Year Study, Pornjit Sonseeda, Thevin Vongpralub, Banyat Laopaiboon

The Thai Journal of Veterinary Medicine

Several reports on the semen characteristics of domestic fowl have indicated that environmental factors, breed and age significantly affect semen quality. There is, however, a paucity of data on effects of environmental factor, breed and age on semen characteristic in Thai indigenous chickens. The aims of the present study were to investigate the effects of annual ambient temperature and humidity, local breeds (i.e. Lueng hang kaow, Pradoo hang dam and Chee), and ages (10 and 18 months) on sperm production and semen quality among Thai native cocks. Thirty-six Thai native cocks were housed under natural environmental conditions, and semen was …

Comparison Of Gentamicin Impregnated Polymethlymetacrylate Bead, Gentamicin Coated Native Calcium Sulfate Bead And Gentamicin Coated High Porous Calcium Sulfate Bead On Osteomyelitis Management In A Rat Model, Chaiyakorn Thitiyanaporn, Pareeya Udomkusonsri, Naris Thengchaisri

The Thai Journal of Veterinary Medicine

Three types of gentamicin beads were compared in a rat osteomyelitis model. The gentamicin beads were prepared in cylinder shape (diameter 2 mm x height 4 mm). Gentamicin impregnated polymethylmethacrylate (GIPMMA) beads were fabricated according to manufacturer’s instructions. Gentamicin coated native calcium sulfate (GNCS) bead and gentamicin coated high porous calcium sulfate (G-HPCS) bead were prepared in laboratory. Osteomyelitis was induced in the rat’s tibias by using methicillin resistance Staphylococcus aureus (MRSA). After 3 weeks of infection, the infected tibias were implanted with GI-PMMA bead, G-NCS bead, G-HPCS bead or sham treatment (control). Radiographic change, white blood cell count and …

Inactivation Of Infectious Bronchitis Virus With Various Kinds Of Disinfectants, Pheemphat Bengtong, Thotsapol Thomrongsuwannakij, Niwat Chansiripornchai

The Thai Journal of Veterinary Medicine

This study aimed to perform an in vitro testing of the efficacy of various kinds of disinfectant on infectious bronchitis virus (IBV). Four kinds of disinfectants including Virusnip, Omnicide, CID2000 and Virkon S were used for the virus inactivation test. After challenge with each strain of IBV for each contact time (30 seconds, 1, 5 and 30 minutes), we recorded number of dead embryo/6 inoculated eggs after incubation. Results showed that there was no significant difference among the contact times except 1:800 Virusnip tested for Tha07 (p < 0.05). For Tha03, there was a significant difference among the disinfectants at 30 seconds and 1 minute of contact times (p < 0.05). For Tha08, there was a significant difference among the disinfectants at 1 minute of contact time (p < 0.05). For Tha09, there was a significant difference among the disinfectants at 5 and 30 minutes of contact times (p < 0.05). For Tha10, there was a significant difference among the disinfectants at 5 minutes of contact time (p < 0.05). Virusnip revealed the ability to inactivate the activity of 9 IBV strains in all exposure times, especially at dilutions of 1 : 100 and 1 : 200.

Discrimination Between Tropical Bed Bug Cimex Hemipterus And Common Bed Bug Cimex Lectularius (Hemiptera: Cimicidae) By Pcr-Rflp, Apiwat Tawatsin, Kittitouch Lorlertthum, Atchara Phumee, Usavadee Thavara, Jotika Boon-Long, Rungfar Boonserm, Padet Siriyasatien

The Thai Journal of Veterinary Medicine

Bed bugs, Cimex hemipterus and Cimex lectularius, are common blood-sucking ectoparasites of human and currently found in many countries around the world. In Thailand, both species have been found mostly in hotels in tourist attraction areas and the insecticide resistance of these insects was also documented. To date, identification of these two bed bug species is based on morphological taxonomy, a technique which requires expertise and in some instance is difficult especially for immature bed bugs or incomplete bed bug samples. In this study, we analyzed the cytochrome c oxidase subunit I (COI) gene of bed bugs, C. hemipterus and …

Viability And Growth Of Preantral Follicles Derived From Cryopreserved Ovarian Tissues Of A Cheetah (Acinonyx Jubatus) Post-Mortem, Grisnarong Wongbandue, Nae Tanpradit, Daraka Thongthainun, Paweena Thuwanut, Kaywalee Chatdarong

The Thai Journal of Veterinary Medicine

This study aimed to investigate freezing effects of ovarian tissues on survival of preantral follicles and observing in vitro growing of preantral follicles retrieved from cryopreserved ovarian cortical tissues of a cheetah post-mortem. After 29-hour cold storage, ovarian cortices were cut into small pieces (2.0 x 2.0 x 1.0 mm3) and allocated to be frozen using a passive cooling container (n = 3 pieces) or vitrification (n=3 pieces). After one year of storage, 43 (10/23) and 21% (12/58) follicles isolated from ovarian tissues cryopreserved using a passive cooling device (slow freezing rate) and vitrification, respectively, were viable (positively stained with …

Endoscopic Evaluation Of Gastric Mucosa To Determine Safety Of Three Chondroprotective Drugs In Healthy Dogs, Korakot Nganvongpanit, Kittipong Kungprathum, Terdsak Yano, Kumpanart Soontornvipart

The Thai Journal of Veterinary Medicine

This randomized, double-blind, placebo-controlled study investigated the effects of glucosamine/chondroitin sulfate, chondroitin sulfate, and diacerein on the gastric mucosa of dogs, and on clinical signs of vomiting and diarrhea. Twenty-one healthy adult dogs were included in the study, and were randomly assigned to seven treatment groups, 3 dogs per group. The effect of those medicines on the gastric mucosa was evaluated by endoscopy on day 3 and day 14, and compared with pre-treatment. Clinical signs, including vomiting and diarrhea, were recorded every day during the study period. The results showed a non-significant effect of glucosamine/chondroitin sulfate, chondroitin sulfate, and diacerein …

Buccal Swab As A Source Of Noninvasive Technique For Genomic Dna Collection In Felidae, Janjira Phavaphutanon, Sudtisa Laopium, Kawin Nanklang, Kaikanoke Sirinarumitr, Kornchai Kornkaewrat, Anuchai Pinyopummin, Jitrakorn Viriyarumpa, Piyawan Suthanmapinunt, Narathip Vorawattanatham

The Thai Journal of Veterinary Medicine

This study was conducted to determine whether buccal cells obtained by swabbing could be a DNA source for genetic analysis, and to compare two preservative media, distilled water and Tris-EDTA (TE), pH 8.0. Buccal cell samples were collected from 30 domestic cats (Felis catus), 4 captive fishing cats (Prionailurus viverrinus) and 8 captive tigers (Panthera tigris). The total concentration of DNA and purity were not statistically different between the preservative media. All samples could be graded as a minimum as high quality DNA (DNA found with A260/A280 ratio >2.0 or < 1.8). In addition, the quality of DNA extracted from both media was successfully amplified for PCR. However, when compared to whole blood samples, total DNA concentration from buccal swab was significantly lower (p < 0.05) .Despite the decrease in DNA yield, buccal swab could be an alternative source for obtaining DNA for genetic analyses. This non-invasive, simple and inexpensive technique eases genetic studies in this family especially in domestic cat from which whole blood samples are difficult to collect.

Ecg Quiz, Chollada Buranakarl, Suwanakiet Sawangkoon, Anusak Kijtawornrat, Thanusorn Phakhawambodee

The Thai Journal of Veterinary Medicine

No abstract provided.

Structural, Antigenic, And Evolutionary Characterizations Of The Envelope Protein Of Newly Emerging Duck Tembusu Virus, Kexoang Yu, Zhi-Zhang Sheng, Bing Huang, Xiuli Ma, Yufeng Li, Xiaoyuam Yuan, Zhuoming Qin, Dan Wang, Suvobrata Chakravarty, Feng Li, Minxun Song, Huaichang Sun

Chemistry and Biochemistry Faculty Publications

Since the first reported cases of ducks infected with a previously unknown flavivirus in eastern China in April 2010, the virus, provisionally designated Duck Tembusu Virus (DTMUV), has spread widely in domestic ducks in China and caused significant economic losses to poultry industry. In this study, we examined in detail structural, antigenic, and evolutionary properties of envelope (E) proteins of six DTMUV isolates spanning 2010–2012, each being isolated from individual farms with different geographical locations where disease outbreaks were documented. Structural analysis showed that E proteins of DTMUV and its closely related flavivirus (Japanese Encephalitis Virus) shared a conserved array …

Osmotic Stress, Not Aldose Reductase Activity, Directly Induces Growth Factors And Mapk Signaling Changes During Sugar Cataract Formation, Peng Zhang, Kuiyi Xing, James Randazzo, Karen Blessing, Marjorie F. Lou, Peter Kador

School of Veterinary and Biomedical Sciences: Faculty Publications

In sugar cataract formation in rats, aldose reductase (AR) actitvity is not only linked to lenticular sorbitol (diabetic) or galactitol (galactosemic) formation but also to signal transduction changes, cytotoxic signals and activation of apoptosis. Using both in vitro and in vivo techniques, the interrelationship between AR activity, polyol (sorbitol and galactitol) formation, osmotic stress, growth factor induction, and cell signaling changes have been investigated. For in vitro studies, lenses from Sprague Dawley rats were cultured for up to 48 hrs in TC-199-bicarbonate media containing either 30 mM fructose (control), or 30 mM glucose or galctose with/without the aldose reductase inhibitors …

Mimicry Epitope From Ehrlichia Canis For Interphotoreceptor Retinoid-Binding Protein 201–216 Prevents Autoimmune Uveoretinitis By Acting As Altered Peptide Ligand, Arunakumar Gangaplara, Chandirasegaran Massilamany, David Steffen, Jay Reddy

Jay Reddy Publications

We report here identification of novel mimicry epitopes for interphotoreceptor retinoid-binding protein (IRBP) 201–216, a candidate ocular antigen that causes experimental autoimmune uveoretinitis (EAU) in A/J mice. One mimicry epitope from Ehrlichia canis (EHC), designated EHC 44–59, induced cross-reactive T cells for IRBP 201–216 capable of producing T helper (Th)1 and Th17 cytokines, but failed to induce EAU in A/J mice. In addition, animals first primed with suboptimal doses of IRBP 201–216 and subsequently immunized with EHC 44–59 did not develop EAU; rather, the mimicry epitope prevented the disease induced by IRBP 201–216. However, alteration in the composition of EHC …

Analysis Of Unannotated Equine Transcripts Identified By Mrna Sequencing, Stephen J. Coleman, Zheng Zeng, Matthew S. Hestand, Jinze Liu, James N. Macleod

Veterinary Science Faculty Publications

Sequencing of equine mRNA (RNA-seq) identified 428 putative transcripts which do not map to any previously annotated or predicted horse genes. Most of these encode the equine homologs of known protein-coding genes described in other species, yet the potential exists to identify novel and perhaps equine-specific gene structures. A set of 36 transcripts were prioritized for further study by filtering for levels of expression (depth of RNA-seq read coverage), distance from annotated features in the equine genome, the number of putative exons, and patterns of gene expression between tissues. From these, four were selected for further investigation based on predicted …

A Joint Matrix Completion And Filtering Model For Influenza Serological Data Integration., Xiao-Tong Yuan, Tong Zhang, Xiu-Feng Wan

CVM Publications

Antigenic characterization based on serological data, such as Hemagglutination Inhibition (HI) assay, is one of the routine procedures for influenza vaccine strain selection. In many cases, it would be impossible to measure all pairwise antigenic correlations between testing antigens and reference antisera in each individual experiment. Thus, we have to combine and integrate the HI tables from a number of individual experiments. Measurements from different experiments may be inconsistent due to different experimental conditions. Consequently we will observe a matrix with missing data and possibly inconsistent measurements. In this paper, we develop a new mathematical model, which we refer to …

Heterogeneous Nuclear Ribonucleoprotein K Supports Vesicular Stomatitis Virus Replication By Regulating Cell Survival And Cellular Gene Expression, Phat X. Dinh, Anshuman Das, Rodrigo Franco, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

The heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a member of the family of hnRNPs and was recently shown in a genome-wide small interfering RNA (siRNA) screen to support vesicular stomatitis virus (VSV) growth. To decipher the role of hnRNP K in VSV infection, we conducted studies which suggest that the protein is required for VSV spreading. Virus binding to cells, entry, and nucleocapsid uncoating steps were not adversely affected in the absence of hnRNP K, whereas viral genome transcription and replication were reduced slightly. These results indicate that hnRNP K is likely involved in virus assembly and/or release from …

Growing South Dakota (Summer 2013), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Fish, Field & Forest: Natural Resource Management Research Covers the Gamut
[Page] 5 Profiles In Leadership: David Willis; Combined Department Creates Interdisciplinary Opportunities
[Page] 6 Research On the Range: Cottonwood Field Station Has Provided Research Data for 106 Years
[Page] 8 Prairie Oasis: Natural Diversity Showcased at Oak Lake Field Station
[Page] 9 Special Section: Investigating in Agriculture & Biological Sciences at SDSU: An Update On the College of Ag’s Enhanced Facilities and Continuing Efforts Important to Helping Address Future Agricultural Challenges and Opportunities.[Page] 13 South Dakota 4-H Update: Skill-a-thons, New Exhibit Hall Planned
[Page] 14 After The …

Envelope Determinants Of Equine Lentiviral Vaccine Protection, Jodi K. Craigo, Corin Ezzelarab, Sheila J. Cook, Chong Liu, David W. Horohov, Charles J. Issel, Ronald C. Montelaro

Veterinary Science Faculty Publications

Lentiviral envelope (Env) antigenic variation and associated immune evasion present major obstacles to vaccine development. The concept that Env is a critical determinant for vaccine efficacy is well accepted, however defined correlates of protection associated with Env variation have yet to be determined. We reported an attenuated equine infectious anemia virus (EIAV) vaccine study that directly examined the effect of lentiviral Env sequence variation on vaccine efficacy. The study identified a significant, inverse, linear correlation between vaccine efficacy and increasing divergence of the challenge virus Env gp90 protein compared to the vaccine virus gp90. The report demonstrated approximately 100% protection …

Tricarboxylic Acid Cycle And One-Carbon Metabolism Pathways Are Important In Edwardsiella Ictaluri Virulence., Neeti Dahal, Hossam Abdelhamed, Jingjun Lu, Attila Karsi, Mark L. Lawrence

CVM Publications

Edwardsiella ictaluri is a Gram-negative facultative intracellular pathogen causing enteric septicemia of channel catfish (ESC). The disease causes considerable economic losses in the commercial catfish industry in the United States. Although antibiotics are used as feed additive, vaccination is a better alternative for prevention of the disease. Here we report the development and characterization of novel live attenuated E. ictaluri mutants. To accomplish this, several tricarboxylic acid cycle (sdhC, mdh, and frdA) and one-carbon metabolism genes (gcvP and glyA) were deleted in wild type E. ictaluri strain 93-146 by allelic exchange. Following bioluminescence tagging of the E. ictaluri ?sdhC, ?mdh, …

The 23rd Annual Research Conference Abstract Booklet, Mulu Aderie Alemu, Nikki Lynn Rogers

University of Gondar Research Conferences

Staff members, postgraduate and senior undergraduate students of the University, invited guests and speakers participated in the conference. The annual conference of the University is meant to share experiences in research activities among juniors and seniors, staff and students, and invited guests. It is also meant to motivate students and young faculty to engage in research and also to initiate and strengthen interdisciplinary collaborations. The findings of the studies and the resulting recommendations are expected to be used in solving the diverse societal problems we have been facing.

Research activities at the University of Gondar are primarily aimed at solving …

Indices Of Myocardial Contractility, Anusak Kijtawornrat

The Thai Journal of Veterinary Medicine

Myocardial contractility is defined as the velocity of cycling of heavy meromyosin cross-bridges. Contractility may be defined also as the velocity of myocardial fiber shortening or change in ventricular dimension during contraction when the fiber shortens or the ventricle contracts against no load. In the clinic; however, myocardial contractility may be estimated by some parameters of systolic function adjusted for the degree of stretch on the fibers (the preload) just before contraction begins and the hindrance to ejection of blood (the afterload). Excitation-contraction coupling is a physicochemical process during which electrophysiological changes (initiated by depolarization of the cell) translate to …

Probable Pig To Duck Transmission Of The Pandemic H1n1 2009 (Ph1n1) And Its Reassortant In Commingling Experimental Condition, Nataya Charoenvisal, Juthatip Keawcharoen, Donruethai Sreta, Siriporn Tantawet, Suphattra Jittimanee, Jirapat Arunorat, Korakrit Poonsuk, Alongkorn Amonsin, Roongroje Thanawongnuwech

The Thai Journal of Veterinary Medicine

Pandemic H1N1 2009 (pH1N1) virus is considered as a low pathogenic influenza virus, however, it rapidly spread among humans and was finally found in the swine population of 6 continents. Interspecies transmissions among different animal species are of interest. In this study, sentinel ducks were commingled with pH1N1 or pandemic H1N1 reassortment virus (rH1N1) inoculated pigs in separate groups. According to the results, both studied viruses were able to cross-species transmit to a few sentinel ducks with mild or no clinical signs. Viral shedding measured by a modified real time RT-PCR detection from the oropharyngeal and cloacal swabs were also …