Medicine and Health Sciences Commons^™

Central Role Of Vasotocin In The Neuroendocrine Regulation Of Stress Responses And Food Intake In Chickens, Gallus Gallus, Gurueswar Nagarajan

Graduate Theses and Dissertations

It is well known that arginine vasotocin (AVT) in birds is involved in physiological homeostasis such as cardiovascular, osmotic regulation as well as reproductive functions. Pertinent to these physiological functions, AVT immunoreactive (-ir) neurons in the hypothalamus have been found associated with hemorrhage, dehydration, oviposition and other physiological regulation. Evidence, however, suggests that AVT also plays significant roles in modulating behavior, memory, stress, and food intake. This dissertation research addresses the latter two neuroendocrine functions of AVT in detail within the chicken brain. First, the functional role of AVT-ir neurons in conjunction with corticotropin releasing hormone (CRH)-ir neurons in the …

Reduction In Activity By Noxious Chemical Stimulation Is Ameliorated By Immersion In Analgesic Drugs In Zebrafish, Javier Lopez-Luna, Qussay Al-Jubouri, Waleed Al-Nuaimy, Lynne U. Sneddon

Anesthesia and Analgesia Collection

Research has recently demonstrated that larval zebrafish show similar molecular responses to nociception to those of adults. Our study explored whether unprotected larval zebrafish exhibited altered behaviour after exposure to noxious chemicals and screened a range of analgesic drugs to determine their efficacy to reduce these responses. This approach aimed to validate larval zebrafish as a reliable replacement for adults as well as providing a high-throughput means of analysing behavioural responses. Zebrafish at 5 days postfertilization were exposed to known noxious stimuli: acetic acid (0.01%, 0.1% and 0.25%) and citric acid (0.1%, 1% and 5%). The behavioural response of each …

Evaluation Of Six Serological Elisa Kits Available In Italy As Screening Tests For Equine Infectious Anaemia Surveillance, Roberto Nardini, Gian Luca Autorino, Charles J. Issel, Richard F. Cook, Ida Ricci, Raffaele Frontoso, Francesca Rosone, Maria Teresa Scicluna

Maxwell H. Gluck Equine Research Center Faculty Publications

Background: ELISAs are known to have a higher diagnostic sensitivity than the agar gel immunodiffusion (AGID) when employed for serological diagnosis of equine infectious anaemia (EIA). For this purpose, an “in-house” and five commercial ELISAs available in Italy were assessed by the National Reference Centre for EIA for their analytic specificity (Sp); precocity, defined as capability of detecting first antibodies produced during a new infection; precision based on repeatability and reproducibility, estimated from the coefficient of variation (CV); accuracy, estimated from multiple K and relative Sp and sensitivity (Se). Two serum panels, positive for non-equine retroviruses and the most frequent …

The Use Of Papain And Benzyl Isothiocyanate As Anthelmintics For Equine Strongyles, Abigail Maxey

Undergraduate Honors Thesis Projects

Seeds of Carica papaya L. (Caricaceae) are a promising source of investigation for a novel anthelmintic to treat equine strongyle infestation. Strongyles are small redworms that attach to the intestinal wall in the equine. They can cause colic, poor hair coat, poor body condition, and, in severe cases, death. The objective of this study was to determine if benzyl isothiocyanate (BITC) and papain, two compounds found in papaya seeds, could be used to prevent migration of third stage cyathostome larvae (L3) in vitro using larval migration inhibition assays. The assays involved incubating larvae in solution, followed by larval migration through …

Evaluation Of Circulating Mirnas During Late Pregnancy In The Mare, Shavahn C. Loux, Kirsten E. Scoggin, Jason E. Bruemmer, Igor F. Canisso, Mats H. T. Troedsson, Edward L. Squires, Barry A. Ball

Veterinary Science Faculty Publications

MicroRNAs (miRNAs) are small, non-coding RNAs which are produced throughout the body. Individual tissues tend to have a specific expression profile and excrete many of these miRNAs into circulation. These circulating miRNAs may be diagnostically valuable biomarkers for assessing the presence of disease while minimizing invasive testing. In women, numerous circulating miRNAs have been identified which change significantly during pregnancy-related complications (e.g. chorioamnionitis, eclampsia, recurrent pregnancy loss); however, no prior work has been done in this area in the horse. To identify pregnancy-specific miRNAs, we collected serial whole blood samples in pregnant mares at 8, 9, 10 m of gestation …

Out In The Cold: Ingredients In Over-The-Counter Cold Medicines Can Wreak Havod On A Trainer's Livelihood And An Owner's Prized Racehorse, Clara Fenger, Tanya Boulmetis, Thomas Tobin

Maxwell H. Gluck Equine Research Center Faculty Publications

No abstract provided.

Trap-Neuter-Return Programs And The Importance Of Associated Public Awareness, Nicolette Sliwa

Biology: Student Scholarship & Creative Works

The focus of this paper is to describe a typical Trap-Neuter-Return (TNR) program and highlight the major pros and cons surrounding this somewhat controversial method of managing the overwhelming free-roaming cat population. The effects of the species’ overabundance on the environment and human health are also discussed. Citing studies completed on TNR programs around the globe as well as mathematical models, the effectiveness of this method is compared to other, currently used alternatives. Finally, it has been suggested that the general awareness level a neighborhood possesses about the true goals of TNR programs has a potentially significant impact on the …

Ebola Virus - Epidemiology, Diagnosis, And Control: Threat To Humans, Lessons Learnt, And Preparedness Plans - An Update On Its 40 Year's Journey, Raj Kumar Singh, Kuldeep Dhama, Yashpal Singh Malik, Muthannan Andavar Ramakrishnan, Kumaragurubaran Karthik, Rekha Khandia, Ruchi Tiwari, Ashok Munjal, Mani Saminathan, Perumal Arumugam, Sunil Kumar Joshi

Bioelectrics Publications

Ebola virus (EBOV) is an extremely contagious pathogen and causes lethal hemorrhagic fever disease in man and animals. The recently occurred Ebola virus disease (EVD) outbreaks in the West African countries have categorized it as an international health concern. For the virus maintenance and transmission, the non-human primates and reservoir hosts like fruit bats have played a vital role. For curbing the disease timely, we need effective therapeutics/prophylactics, however, in the absence of any approved vaccine, timely diagnosis and monitoring of EBOV remains of utmost importance. The technologically advanced vaccines like a viral-vectored vaccine, DNA vaccine and virus-like particles are …

Growing South Dakota (Spring 2017), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

This issue contains the 2016 South Dakota Agricultural Experiment Station Annual Report.

[Page] 3 Opportunities With Oats SDSU Researchers Strive to Improve Varieties [Page] 6 Stopping Stem Canker Plant Pathologists Making Progress To Keep This Disease At Bay
[Page] 7 Disease Detectives Vet Scientists Researching Another Emerging Virus [Page] 8 Community Support Devastating West River Fire Brings Ag Industry Together
[Page] 10 Research In Progress Dakota Lakes Research Farm Investigates Three Big Ideas
[Page] 14 Water Quality Quest Understanding E. Coli Behavior Aims To Aid Water Quality In Rivers & Streams
[Page] 15 Verifying Ag Land Valuation Researchers Assist In …

Exogenous Lactobacilli Mitigate Microbial Changes Associated With Grain Fermentation (Corn, Oats, And Wheat) By Equine Fecal Microflora Ex Vivo, Brittany E. Harlow, Laurie M. Lawrence, Patricia A. Harris, Glen E. Aiken, Michael D. Flythe

Animal and Food Sciences Faculty Publications

Cereal grains are often included in equine diets. When starch intake exceeds foregut digestion starch will reach the hindgut, impacting microbial ecology. Probiotics (e.g., lactobacilli) are reported to mitigate GI dysbioses in other species. This study was conducted to determine the effect of exogenous lactobacilli on pH and the growth of amylolytic and lactate-utilizing bacteria. Feces were collected from 3 mature geldings fed grass hay with access to pasture. Fecal microbes were harvested by differential centrifugation, washed, and re-suspended in anaerobic media containing ground corn, wheat, or oats at 1.6% (w/v) starch and one of five …

Leaders Of The Pack: Women And The Future Of Veterinary Medicine, Julie Kumble, Donald F. Smith

Purdue University Press Book Previews

Veterinary medicine has undergone sweeping changes in the last few decades. Women now account for 55 percent of the active veterinarians in the field, and nearly 80 percent of veterinary students are women. However, average salaries have dropped as this shift has occurred, and even with women in the vast majority, only 25 percent of leadership roles are held by women.

These trends point to gender-based inequality that veterinary medicine, a profession that tilts so heavily toward women, is struggling to address. How will the profession respond? What will this mean for our students and schools? What will it mean …

Leaders Of The Pack: Women And The Future Of Veterinary Medicine, Julie Kumble, Donald Smith

New Directions in the Human-Animal Bond

Veterinary medicine has undergone sweeping changes in the last few decades. Women now account for 55 percent of the active veterinarians in the field, and nearly 80 percent of veterinary students are women. However, average salaries have dropped as this shift has occurred, and even with women in the vast majority, only 25 percent of leadership roles are held by women. These trends point to gender-based inequality that veterinary medicine, a profession that tilts so heavily toward women, is struggling to address. How will the profession respond? What will this mean for our students and schools? What will it mean …

Effects Of Temperature And Diet In Stable Fly (Diptera: Muscidae) Development, Melina Florez-Cuadros

Department of Entomology: Dissertations, Theses, and Student Research

Stable flies are among the most important blood feeding ectoparasites of cattle worldwide. The livestock industry is losing more than $2 billion dollar annually in the United States alone. Moist decaying vegetative material with bacteria supports larval development. Temperature and diet quality are important factors for stable fly development, affecting development rates, adult survival, and size, but how temperature affects adult size and interacts with nutrition is unknown. This study characterizes the relationship of temperature and diet quality in the development of stable flies. Two statistical models were used to analyze the effect of temperature alone (Lysyk, 1998) and temperature-diet …

Exploring Difference In Subgingival Microbial Communities In Dog And Human Periodontal Diseases Using Dgge Technique, Phutsa Sanguansermsri, Kongthawat Chairatvit, Sittiruk Roytrakul, Sombhun Doungudomdacha, Rudee Surarit

The Thai Journal of Veterinary Medicine

Periodontal diseases are the most common infectious diseases of dogs. Up to now, the knowledge about periodontopathic bacteria in dogs remains sparse. The objectives of this study were to apply the Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) technique, cloning and 16S rRNA gene sequencing to the investigation into subgingival plaque bacteria from healthy and periodontitis dogs; and compare DGGE patterns from the subgingival plaque of dogs and humans. Sixty-eight bacterial species were detected from subgingival plaque of 12 Poodles in Thailand and the predominant genus was Porphyromonas (n=11). Porphyromonas gulae and Treponema denticola were the most common microflora in …

Occurrence Of Mastitis And Associated Pathogens With Antibiogram In Animal Population Of Peshawar, Pakistan, Rafiullah Rafiullah, Mirza Ali Khan, Muhammad Shafee, Ali Akbar, Anwar Ali, Muhammad Shoaib, Faiza Ashraf, Naimatullah Khan

The Thai Journal of Veterinary Medicine

Mastitis is a devastating disease of dairy animals in Pakistan that occurs in clinical and subclinical forms. Various bacterial pathogens are associated with mastitis and the susceptibility of these pathogens remains inconsistent to various antibacterial drugs. This study was aimed to assess the prevalence of mastitis in and around the city of Peshawar, Pakistan. A total of 2,791 milk samples (Bovine, Ovine and Caprine) were subjected to White side test and Surf field mastitis test. Positive milk samples for mastitis were cultured on different selective culture media for identification of prevalent bacterial pathogens. Antibiogram of these organisms was evaluated against …

Dna Barcoding Of Chinese Native Chicken Breeds Through Coi Gene, Hongyan Cui, Fahar Ibtisham, Chong Xu, Hanguang Huang, Ying Su

The Thai Journal of Veterinary Medicine

This study was done to investigate the viability and effectiveness of cytochrome c oxidase I (COI) gene in DNA barcoding for identification of Chinese native chickens. Two sequences of the COI gene of 64 chickens of four Chinese native breeds (16 of each breed) were analyzed by amplification and sequencing. Results showed that the bar1 sequence of COI had lower polymorphism compared to bar2. Bar1 had 1 polymorphic site and 2 haplotypes, while bar2 had 4 polymorphic sites and 8 haplotypes. The nucleotide polymorphism (Pi) in bar2 ranged from 0.00102 to 0.00305. The DNA taxonomy of bar2 was consistent with …

Reproductive Control In Elephant: A Tool For Population And Aggression Management, Chaleamchat Somgird, Janine L. Brown, Chatchote Thitaram

The Thai Journal of Veterinary Medicine

Although Asian elephant is listed among the endangered species, the number of populations is over the carrying capacity in some areas, resulting in human-elephant conflict, as well as African elephants. High aggression associated with musth and female reproductive pathology are observed in captive elephants. Thus, population and aggression management through reproductive control is an alternative method for mitigating these problems. This article reviews methods of reproductive control in both Asian and African elephants with an overview of male and female reproductive physiology. Hormonal control and immunocontraception, i.e. porcine zona pellucida and gonadotropin releasing hormone (GnRH), are described for the control …

Improved Growth Performance By Type 2 Porcine Reproductive And Respiratory Syndrome Virus (Prrsv)-Based Modified Live Vaccine In A Herd With Concurrent Circulation Of Type 1 And Type 2 Prrsv, Ikjae Kang, Hei Suk Kang, Jiwoon Jeong, Changhoon Park, Seeun Kim, Kyuhyung Choi, Su-Jin Park, Sung-Min Hwang, Beomseok Oh, Sung-Hoon Kim, Byunghak Kang, Chanhee Chae

The Thai Journal of Veterinary Medicine

The objective of this study was to evaluate type 2 porcine reproductive and respiratory syndrome virus (PRRSV)-based modified live vaccine in a herd with concurrent circulation of type 1 and type 2 PRRSV. Type 2 PRRSV-based modified live vaccine improved average daily gain by 48.24 grams/pig/day (631.17 grams/pig/day in the vaccinated group vs 582.93 grams/pig/day in the unvaccinated group; P < 0.05). Pathological examination indicated that the vaccination effectively reduced microscopic lung lesions compared with the control animals. The protection of this vaccine against type 1 and type 2 PRRSV provides clinical control of co-infection with both genotypes under field conditions.

Sequencing Characterization And Expression Analysis Of Interferon Gamma (Ifn-Γ) In Rusa Deer (Rusa Timorensis) And Thai Cervidae, Sirinart Chaichanathong, Worawidh Wajjwalku, Manakorn Sukmak, Boripat Siriaroonrat, Tarasak Nipanunt, Nikorn Thongtip

The Thai Journal of Veterinary Medicine

To date, the interferon gamma (IFN-γ) assay has become an important diagnostic tool which is applied alternatively to define infection of intracellular pathogens. In this study, the molecular characteristics of IFN-γ in Rusa deer (Rusa timorensis), Hog deer (Axis porcinus), Sambar deer (Rusa unicolor) and Eld’s deer (Rucervus eldii) were determined. Among the cervid family, the analysis of IFN-γ cDNA sequences indicated 5 polymorphism nucleotide positions. Deduced IFN-γ amino acid compositions among the cervid group were negligibly different (98-100% homology). Preliminary quantification of IFN-γ mRNA expression in the cervidae by reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) was demonstrated. …

Localized Histiocytic Sarcoma In A Captive Capybara (Hydrochoerus Hydrochaeris), Saralee Srivorakul, Kittikorn Boonsri, Thewarach Vechmanus, Pinich Boonthong, M. Gerard O’Sullivan, Kidsadagon Pringproa

The Thai Journal of Veterinary Medicine

An irregular, subcutaneous mass (2x3x2 cm) located between the first and second digits of the left hindlimb of a nine-year-old, male, captive capybara (Hydrochoerus hydrochaeris) was biopsied. Microscopically, the mass scattered throughout the dermis. The neoplastic cells were arranged in solid-sheet patterns and comprised of pleomorphic histiocytic cells with pleomorphic nuclei and abundant eosinophillic cytoplasm. Mitotic figures were 2-3 cells/HPF. Numerous, multinucleated, giant cells, up to 10 nuclei/cells, were also prominently observed and found scattered throughout the mass. Immunohistochemically, the neoplastic cells were strongly positive for vimentin and ionized calcium-binding adaptor molecule 1 (Iba-1), while immunoreactivity for cytokeratin was negative. …

What Is Your Diagnosis?, Chutimon Thanaboonnipat, Nan Choisunirachon

The Thai Journal of Veterinary Medicine

No abstract provided.

Relationship Of Temperature-Humidity Index With Milk Production And Feed Intake Of Holstein-Frisian Cows In Different Year Seasons, Tibor Könyves, Nebojša Zlatković, Nurgin Memiši, Dragomir Lukač, Nikola Puvača, Milan Stojšin, Andras Halász, Branislav Miščević

The Thai Journal of Veterinary Medicine

The objective of this study was to assess the relationship of temperature-humidity index (THI) with milk production, feed intake and feed efficiency of Holstein-Frisian cows in different seasons of the year. Five hundred and sixty three cows were monitored in spring, 557 cows in summer, 594 cows in autumn and 567 cows in winter, for a total period of two years. In contrast to the spring, autumn and winter periods, the summer period was characterized by heat stress conditions. Average T and THI exceeded the 25°C and 72 critical points, respectively, on all, 90% and 93% of test days for …

Antiviral Activity Of Thymus Vulgaris And Nepeta Cataria Hydrosols Against Porcine Reproductive And Respiratory Syndrome Virus, Kraijak Kaewprom, Yu-Hsin Chen, Chuen-Fu Lin, Ming-Tang Chiou, Chao-Nan Lin

The Thai Journal of Veterinary Medicine

Porcine reproductive and respiratory syndrome (PRRS) is characterized by reproductive failure of sows and respiratory problems of nursery and growing pigs. Current management strategies mainly focus on preventing PRRS virus (PRRSV) infection through vaccination. However, the currently available modified live vaccines are not sufficient to eradicate the virus and do not provide complete immunity from infection. In search of agents that may be proven clinically effective against North American PRRSV (NA-PRRSV or US-PRRSV) infection, 42 natural herb hydrosols were purified and subjected to screening of their antiviral activity against two US-PRRSV strains in MARC-145 cells. Based on results obtained from …

Presence Of Enterobacteria Producing Extended-Spectrum Β-Lactamases And/Or Carbapenemases In Animals, Humans And Environment In India, Fateh Singh, S.D. Hirpurkar, Sanjay Shakya, Nidhi Rawat, Prashant Devangan, Foziya Farzeen Khan, Sunil Kumar Bhandekar

The Thai Journal of Veterinary Medicine

The objective of this study was to isolate antimicrobial resistant bacteria from animals, humans and environment, and to characterize them based on phenotypic attributes of resistance. A total of 521 samples were collected from animals (284), humans (69) and environment (168) which resulted in isolation of 615 non-duplicate resistant isolates (to either amoxycillin-clavulanate, ceftazidime, cefixime, meropenem, or imipenem). Antimicrobial susceptibility test (AST) revealed the highest rate of resistance to cefixime (49.3%) followed by ceftazidime (45%), meropenem (38.1%), amoxicillin-clavulanate (35.3%), and imipenem (13%). Identification recorded Escherichia coli (54.6%), Klebsiella spp. (17%), Citrobacter spp. (7.5%), Enterobacter spp. (6.7%), Acinetobacter spp. (3.0%), Serratia …

Molecular Characteristics Of Vp2 Gene From Wild-Type Infectious Bursal Disease Viruses (Ibdvs) In Saudi Arabia, Mahmoud H A Mohamed, Mahmoud M. Ismail, Ibrahim M. El-Sabagh, Abdul-Rahman S. Al-Ankari

The Thai Journal of Veterinary Medicine

Infectious bursal disease (IBD) is caused by a virus related to the family Birnaviridae. The virus is a worldwide pathogen and causes immunosuppression and great losses in broiler flocks. In this study, molecular characterization of two Saudi Arabian IBDVs (IBD01/14/SA and IBD02/14/SA) was carried out by reverse transcriptase polymerase chain reaction (RT-PCR) and partial sequence of hypervariable region of VP2 gene. Nucleotide sequences of 740-bp PCR product of the Saudi Arabian isolates were compared with the corresponding nucleotide sequence of 56 reported IBDVs representing different pathotypes from different continents. Phylogenetic analysis showed that the Saudi Arabian isolates were split from …

Treatment Outcomes Of Canine Nasal Tumor Irradiation, Thitsana Ingkasri, Wuttiwong Theeraphun, Waraporn Aumarm

The Thai Journal of Veterinary Medicine

This study aimed to determine the treatment response of canine nasal tumor treated with only linear accelerated radiation therapy (LINAC) using volumetric measurement criteria at Kasetsart University Veterinary Teaching Hospital between May 2013 and August 2015. Medical records of the patients including age, breed, sex, histopathological diagnosis, clinical stage, types of radiation therapy and complications of treatment were recorded. All 20 dogs were irradiated with total dose ranging from 36 to 54 Gray (Gy). Computed tomography was performed with all dogs before and three months after treatment in order to evaluate tumor volume change. Results revealed that median survival time …

Relationships Between Transferrin And Transferrin Receptor (Tfr) Expression In Dogs With Malignant Oronasal Tumors, Sekkarin Ploypetch, Anudep Rungsipipat, Prapruddee Piyaviriyakul, Nan Choisunirachon, Pussadee Makoom, Chanin Kalpravidh

The Thai Journal of Veterinary Medicine

Malignant cells require a large amount of iron for cell growth. Transferrin transfers iron from blood to cells by reacting with transferrin receptor (TfR). In humans, transferrin and transferrin receptor correlate with oral squamous cell carcinoma (OSCC); however, the association with canine oral tumor has not been studied. This study aimed to investigate saliva and serum transferrin and tissue transferrin receptor in canine OSCC and oral melanoma (OM). Saliva and serum transferrin samples were collected pre- and 14 days post-operation, compared with healthy control and evaluated by a sandwich ELISA assay. Serum transferrin concentration in the experimental group both before …

Effects Of Ivabradine On Left Ventricular Function In Dogs With Congestive Heart Failure, Sajika Sri-Ngam, Soontaree Petchdee

The Thai Journal of Veterinary Medicine

Ivabradine is an essential drug administered in current standard therapy for human heart failure and is expected to be beneficial to congestive heart failure dog. Ivabradine is a selective If Channel inhibitor. It reduces cardiac pacemaker activity and slows heart rate. There are a few reports of ivabradine in veterinary applications. The aim of this study was to investigate the effects of ivabradine on left ventricular function in dogs with congestive heart failure. Thirteen client-owned dogs with systolic and/or diastolic heart failure were included in this study. The dogs were assigned to two groups: (i) the active control group, given …

Effects Of The Higher Parity And Litter Size On Piglet Birth Weight And Survival Rate Of Later Born Piglets, Supatee Kitkha, Alongkot Boonsoongnern, Nattavut Ratanavanichrojn, Pichai Jirawattanapong, Anuchai Pinyopummin

The Thai Journal of Veterinary Medicine

The objectives of this study were to evaluate the effects of higher parity and litter size (LS) on piglet birth weight (BW) and to compare the BW and survival rate of piglets born in different birth orders (BO). The study was conducted at a commercial pig farm in Thailand. Data from 230 sows and 3,500 piglets were analyzed including parity, litter size (LS), average birth weight (AvBW), coefficient of variation of piglets’ BW (%CV), standard deviation of piglets’ BW (SD), percentage of small piglets (%Small), individual piglet’s BW, piglet birth order (BO; 1-10, 11-16, 17-25), sex and characteristics at birth …

Effect Of Tlr4 Antagonist On Immune Responses Of Bovine Endometrial Cells, Sroisuda Chotimanukul, Theerawat Swangchan-Uthai, Junpen Suwimonteerabutr, Mongkol Techakumphu

The Thai Journal of Veterinary Medicine

Bovine endometritis is one of the most common reproductive diseases and causes important economic losses. Lipopolysaccharide (LPS) from Gram-negative bacteria is recognized by Toll-like receptor 4 (TLR4). Binding of LPS and TLR4 mediates the expression of proinflammatory cytokines and chemokines. This study aimed to access the effect of TLR4 antagonist (RsLPS) on the immune responses of bovine endometrial cells. Primary culture of bovine endometrial cells were challenged with LPS (100 ng/ml) after treated with and without RsLPS (5000 ng/ml). Endometrial cells without RsLPS and LPS treatment served as control. The mRNA expression of TLR4, CD14, TNF, IL1B and IL8 were …