Medicine and Health Sciences Commons^™

Comparison Of The In Vitro Anticancer Effect Of Habanero Pepper Extract Containing Capsaicin With That Of Pure Capsaicin In Selected Dog Neoplastic Cell Lines, Lukasz Adaszek, Olga Slabczy?Ska, Pawel Lyp, Dagmara Gadomska, Jerzy Zietek, Dorota Rozanska, Maciej Orzelski, Anna Smiech, Marta Staniec, Dorota Krasucka, Stanislaw Winiarczyk

Turkish Journal of Veterinary & Animal Sciences

The aim of this article was to assess the anticancer effectiveness of habanero pepper extract containing capsaicin on two dog cancer lines, DAN (fibroblasts isolated from osteosarcoma) and D-17 (epithelium cells of osteosarcoma obtained from pulmonary metastatic tumors), under in vitro conditions. The experiment was carried out in a suspension of D-17 and DAN line cells with a density of 12 × 105 cells/mL in a culture fluid with 10% of FBS. After 24 h of incubation (37 °C, 5% CO2), the fluid above the culture was removed, and diluted habanero pepper extract was added. The concentrations of capsaicin in …

Identification Of Polymorphism In The Bovine Interleukin-17a Gene And Its Association With Mastitis In Polish Holstein?Friesian Cattle, Magdalena Dusza, Joanna Pokorska, Dominika Ku?Aj, Joanna Makulska

Turkish Journal of Veterinary & Animal Sciences

The main aim of this study was to identify the association between the single nucleotide polymorphism (SNP) in the bovine interleukin-17A (IL-17A) gene and mastitis resistance. Additionally, the connection between the identified polymorphism and the somatic cell score (SCS) was tested. Two hundred and six Polish Holstein?Friesian cows were genotyped using the PCR-RFLP method. One SNP mutation (c.126G>A) was identified within the coding sequence of the analyzed gene. Results of logistic regression analysis displayed no significant association between the IL-17A genotype and clinical mastitis occurrence (Wald chi-square test = 0.74; P = 0.69). The GLM analysis demonstrated that genotype …

The Comparison Of Anesthetic Potency And Toxicity Of 2-Phenoxyethanol And 1-Phenoxy-2-Propanol For Juvenile Common Carp, Ewa Czerniak, Piotr Gomulka, Jakub Dagowski

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate anesthetic potency and acute toxicity of 2-phenoxyethanol (2PE) and 1-phenoxy-2- propanol (PP) for common carp. During efficacy tests, individual phases of anesthesia and recovery, as well as behavior and survival rates, were determined. As effective time for anesthesia and recovery, 3 and 10 min respectively were chosen. Determined efficient concentrations were 600 mg L?1 for 2PE and 415 and 460 mg L ?1 for PP. Acute toxicity tests were performed according to OECD Norm Acute Toxicity Tests for Fish No. 203. To establish the 96-h LC50 of PP and 2PE, juvenile carps …

Comparison Of Igg And Semiquantitative Tests For Evaluation Of Passive Transfer Immunity In Calves, Onur Topal, Hasan Batmaz, Zafer Meci̇toğlu, Ender Uzabaci

Turkish Journal of Veterinary & Animal Sciences

Serum immunoglobulin (IgG) and semiquantitative tests are used for the evaluation of passive transfer immunity (PTI) in calves. We aimed to evaluate PTI in calves by using a Brix refractometer, total protein (TP), gamma-glutamyl transferase (GGT), and glutaraldehyde coagulation test (GCT) on days 1, 3, and 7 after birth; to compare the results with serum IgG; and to evaluate which day these tests are given on will give the best results. The blood samples were collected from 60 Holstein dairy calves on day 0, just after birth, and on the 1st, 3rd, and 7th days after birth. The serum IgG …

Avian Salmonella Enteritidis Serovar Enteritidis With Clpp Deletion Is Attenuated And Highly Immunogenic In Chickens, Xuhua Ran, Zhiyuan Yang, Guagnxi Shao, Xiaobo Wen, Siguo Liu, Hongbo Ni

Turkish Journal of Veterinary & Animal Sciences

Salmonella enterica subsp. enterica serovars are leading causative agents of gastroenteritis in humans and numerous animal species worldwide. The ClpP from Salmonella enterica is responsible for regulation under various stress conditions. We were interested in the effect of ClpP deletion mutation on attenuation of Salmonella enterica subsp. enterica serovar Enteritidis (SE) in chickens. Therefore, SM-6/Δ ClpP , deletion of the ClpP gene from a wild-type SE-6 (SM-6) strain, was generated using the phage &lamda; Red homologous recombination. Compared with SM-6, SM-6/&DeltaClpP represented a retarded growth rate and lower concentration in LB broth with and without 5% NaCl or lower pH …

Isolation Of Multidrug Resistant Coliforms And Their Bacteriophages From Swine Slurry, Adrian Augustyniak, Bartlomiej Grygorcewicz, Pawe? Nawrotek

Turkish Journal of Veterinary & Animal Sciences

Slurry management is a contemporary agricultural problem. Although liquid manure is a valuable fertiliser, it may pose a microbiological threat due to spreading of bacteria resistant to various antibiotics. Fermentation has been proposed as a solution to this problem, although it does not fully eliminate the antibiotic-resistant strains. The presence of gram-negative rods resistant to antimicrobials may cause an uncontrolled transmission of resistance factors and genes in the natural environment. Therefore, a biocontrol system is necessary to prevent this phenomenon. The aim of this study was to detect multidrug-resistant (MDR) bacteria and their lytic bacteriophages in raw swine slurry. For …

Genetic Diversity Of Different Avian Strains Of Pasteurellamultocida Recovered During Four Outbreaks In India, Kumaragurubaran Karthik, Vishwanathan Dillibabu, Ramalingam Mahaprabhu, Ramasamy Bharathi, Kaliyaperumal Manimaran, Kulasekaran Shoba

Turkish Journal of Veterinary & Animal Sciences

Pasteurella multocida causes fowl cholera in avian species and type A is predominantly reported from outbreaks of fowl cholera. Biochemical, serological, and molecular methods are employed for its diagnosis and typing. Utilizing rapid molecular tools like repetitive extragenic palindromic (REP) PCR and enterobacterial repetitive intergenic consensus (ERIC) PCR, P. multocida isolates from four outbreaks (three from Chennai and one from Ahmadabad, India) were characterized and typed to determine their relationships. A total of 36 isolates were recovered from the outbreaks, including one isolate from a parakeet, which was also subjected to characterization by conventional and molecular analysis. All of the …

Simple Hierarchical And General Nonlinear Growth Modeling In Sheep, Mostafa Ghaderi_Jzefrehei, Farjad Rafeie, Mohammad Reza Bahreini Behzadi, Sajad Nazari, Mustafa Dolatabadi, Farhad Samadian, Thomas M R Maxwell, Hamed Amirpour Najafabadi

Turkish Journal of Veterinary & Animal Sciences

Differential equations and advanced statistical models have been used to predict growth phenomena. In the present study, general nonlinear growth functions such as von Bertalanffy, Gompertz, logistic, and Brody, along with hierarchical modeling were applied to investigate the phenotypic growth pattern of Iranian Lori-Bakhtiari sheep. Growth data from 1410 Lori- Bakhtiari lambs were used in the present study. The results showed that the Brody function outperformed the other three nonlinear growth functions. In addition, including hierarchical growth modeling results allowed the adoption of many random effect structures, suggesting that hierarchical growth modeling has a useful role in growth data modeling. …

Scenario Of Porcine Cysticercosis And Human Taeniasis In Maharashtra State, India, Vilas Vaidya, Ashish Paturkar, Ravindra Zende, Mukulesh Gatne, Dhananjay Dighe, Rupesh Waghmare, Shilpa Laxmanrao Moon, Sujata Bhave, Priyanka Jadhav, Nilam Wavhal

Turkish Journal of Veterinary & Animal Sciences

Cysticercosis/taeniasis is one of the neglected parasitic zoonoses in developing countries like India. A study was conducted for a period of 7 years from 2010 to 2017 during which 13,596 pig and 1238 human samples were screened to learn the exact scenario of cysticercosis/taeniasis in the different regions of Maharashtra State. Prevalence of cysticercosis/taeniasis in pigs and humans was recorded using various diagnostic methods such as meat inspection and serological methods involving ELISA. The overall prevalence of cysticercosis in pigs was found to be 0.88% by postmortem examination and 0.9% by PCR assay, whereas prevalence of taeniasis in humans was …

Cadmium Affects The Development Of Somites In Chick Embryos(Gallus Gallus Domesticus) Under In Vitro Conditions, Samira Kazemi, Naser Mahdavi Shahri, Roya Lari, Fatemeh Behnam Rassouli

Turkish Journal of Veterinary & Animal Sciences

Cadmium is one of the most toxic metals that has high environmental stability and can hardly be converted into substances of low toxicity. In the present study, the effects of cadmium on the development of somites of chick embryos under in vitro conditions were investigated. First, fertilized eggs were incubated until they reached the stage of 15?20 somites. The embryos were then separated from the yolk by using the window technique. The somites were separated by insulin needles under a stereomicroscope. The separated somites were then transferred to Dulbecco?s modified Eagle?s medium (DMEM) stock culture and treated with different concentrations …

Bilateral Radial Carpal Bone Luxation And Its Treatment In A Dog, Didar Aydin Kaya

Turkish Journal of Veterinary & Animal Sciences

Radial carpal bone luxation is rarely encountered in dogs. A literature review did not reveal any case of bilateral radial carpal bone luxation or its treatment. In the current case study, the process and result of the treatment of a dog with bilateral radial carpal bone luxation, brought to the İstanbul University Faculty of Veterinary Medicine?s Department of Surgery is presented. Treatment of luxation was achieved by using screws and cerclage wire. The long-term follow-up of this patient was limited to only 2 months since the dog was not brought back to the clinic.

The Effect Of Daily Milk Production On The Milk Composition And Energymanagement Indicators In Holstein?Friesian And Simmental Cows, Krzysztof Mlynek, Beata Gowiska, Ewa Salomonczyk, Jolanta Tkaczuk, Wojciech Stys

Turkish Journal of Veterinary & Animal Sciences

The present study investigated the effect of daily milk production on the content of crucial milk components, body condition, and the level of energy management parameters in the blood and milk in Holstein?Friesian (HF) and Simmental (SIM) cows. Within each breed, the animals were divided into two groups: the HF-L group was HF cows with daily milk production of ≤31 kg and the HF-H group was those with daily milk production of >31 kg. The SIM-L was SIM cows with daily milk production of ≤27 kg and the SIM-H group was those with daily milk production of >27 kg. The …

He Scenario Tree Epidemiological Model In Estimation Effects Of B. Melitensis Rev 1 Vaccination On Disease Prevalence, Sabina Seric Haracic, Nihad Fejzic, Ermin Saljic, Dzenita Hadzijunuzovic Alagic, Mo Salman

Turkish Journal of Veterinary & Animal Sciences

minant brucellosis. The stochastic scenario tree model was used to simulate vaccination and surveillance implementation during 8 years. Outputs were annual proportions of true positive, true negative, false positive, and false negative flocks. Vaccination coverage was described by the Pert distribution with average 70% and min and max 50% and 80%, respectively. Effects of risk factors (high prevalence-areas and transhumance) were considered. The model was separately simulated for three levels of initial average flock prevalence (2%, 5%, and 10%). In the following years, flock prevalence arising from the fitted distribution of the false negative flocks from the previous year increased …

Evaluation Of Biochemical Parameters And Oxidative Stress In Sheep Naturally Infected With Dicrocoelium Dendriticum And Hydatid Cysts, Mi̇yase Çinar, Meral Aydeni̇zöz, Sami̇ Gökpinar, Güzi̇n Çamkerten

Turkish Journal of Veterinary & Animal Sciences

The purpose of the study was to investigate the lipid peroxidation and antioxidant status as well as biochemical profile in sheep naturally infected with Dicrocoelium dendriticum and hydatid cysts. Ten sheep naturally infected with D. dendriticum, 10 sheep naturally infected with hydatid cysts, and 10 healthy sheep were used. The blood and visceral organs of sheep brought to a slaughterhouse in the region of Kırıkkale were checked for D. dendriticum and hydatid cysts. Plasma total protein, albumin, total bilirubin, total cholesterol, Ca, Mg, inorganic P, Cu, Zn, vitamin A, vitamin C, vitamin E, ß-carotene, AST, ALT, GGT, ALP, erythrocyte MDA …

The Protective Effects Of Chrysin And Flunixin Meglumine Against Excess Copper In Male Rats, Pinar Tatli Seven, Burcu Gül Baykalir, İsmai̇l Seven, Tuba Parlak Ak, Neşe Başak Türkmen, Hakan Güllüoğlu

Turkish Journal of Veterinary & Animal Sciences

Thirty-six Sprague Dawley male rats were housed in individual cages and randomly divided into six groups: control, copper sulfate (Cu; 500 ppm body weight (BW)/day), flunixin meglumine (FM; 2.2 ppm BW/day), chrysin (chrysin; 50 ppm BW/day), copper sulfate + FM (Cu + FM; 500 ppm BW/day of copper sulfate and 2.2 ppm BW/day of FM), and copper sulfate + chrysin (Cu + Chrysin; 500 ppm BW/day of copper sulfate and 50 ppm BW/day of chrysin). Feed intake (FI) in the Cu + Chrysin group significantly increased in comparison with that of the Cu group (P < 0.01). Cu excess significantly increased malondialdehyde, indicating oxidative stress. Chrysin and FM administration significantly decreased malondialdehyde levels and increased the superoxide dismutase and catalase activities in the liver and kidney tissues (P < 0.001). Serum TNF-α levels were significantly lower in the Cu + FM and Cu + Chrysin groups in comparison to the Cu group (P < 0.001). It was seen that FM and chrysin treatments alleviated degeneration, necrosis, and apoptosis in the liver and kidney tissues of the Cu-treated rats. Chrysin appeared to ameliorate the adverse effects on FI and liver and kidney tissues by scavenging where the free radicals are located and increasing the activity of antioxidants.

Complications Of Ultrasound-Guided Cystocentesis In Companion Animals: 21 Cases (2005-2016), Sabrina Manfredi, Cibele Carvalho, Paolo Fonti, Giacomo Gnudi, Francesca Miduri, Martina Fabbi, Eleonora Daga, Manuela Ravera, Simona Morabito, Edoardo Auriemma, Ilaria Parroccini, Mattia Bonazzi, Antonella Volta

Turkish Journal of Veterinary & Animal Sciences

Complications of cystocentesis in dogs and cats are seldom reported. The aim of this study was to describe clinical and ultrasonographic features of complications after diagnostic cystocentesis. Medical records of animals that underwent ultrasound-guided diagnostic cystocentesis (UGDC) were retrospectively reviewed to describe adverse events. The inclusion criteria were: ultrasound report describing the complication, signalment, clinical history, urine culture, and surgical exploration or necroscopic findings if present. Images of ultrasonographic examination were reviewed. Complications were classified as minor and major. Minor complications were defined as self-limited events; major complications were those that led to prolonged hospitalization and required intensive therapy or …

Molecular Determination Of Methicillin Resistance Meca And Virulence Coa Genes In Staphylococcus Aureus From Pyogenic Clinical Cases Of Companion Animals In India, Ritika Yadav, Amit Kumar, Vinod Singh, Jayshree Singh, Sharad Kumar Yadav

Turkish Journal of Veterinary & Animal Sciences

Out of 100 pyogenic clinical cases of companion animals, 40 samples revealed Staphylococcus aureus confirmed by genomic amplification of 280-bp amplicons of the nuc gene. The presence of resistance and virulence determinants was confirmed for the genomic and plasmid mec, van, and coa genes. Out of 40 isolates of S. aureus, 23 isolates revealed amplification of 533-bp mecA genes on genomic DNA. These included 4, 14, and 5 isolates of cattle, buffalo, and dogs, respectively. On amplification for the coa gene, 19 isolates revealed amplicons of variable size and number on genomic DNA. The numbers of coa-positive isolates were 4, …

Assessment Of Tumor-Induced Pain And C-Reactive Protein Levels In Dogs With Caninetransmissible Venereal Tumors, Waqas Ahmad, Muti-Ur- Rehman, Aftab Ahmad Anjum, Gulbeena Saleem, Ubaid Ur Rehman Zia, Muhammad Azeem, Fahad Akram

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the impact of tumor volume on generalized behavior, pain perception, and serum C-reactive protein (CRP) in dogs with canine transmissible venereal tumors (CTVTs). Pain scoring was performed using the Glasgow Composite Measure Pain Scale Short-Form. Cytomorphology of the tumors was studied through fine-needle aspiration and histopathology. Molecular identification of CTVTs was carried out using PCR assays based on LINE-1/c-myc. Hematological profiles including complete blood count and CRP were analyzed using an automatic hematology analyzer and immunoturbidimetric assays, respectively. Chi-square and regression analysis statistics were used for data analysis. Results of the study …

Uterine Prolapse In A Pregnant Cat, Zeynep Günay Uçmak, Meli̇h Uçmak, Ali̇ Can Çeti̇n, Çağatay Tek

Turkish Journal of Veterinary & Animal Sciences

A 3-year-old, 2.5-kg British shorthair cat with dystocia and protrusion of a mass through the vulva was presented to the Obstetrics and Gynaecology Clinic, Faculty of Veterinary Medicine, İstanbul University. Upon clinical examination, a complete uterine prolapse was diagnosed. Because of a blood blister and oedema in the uterine horns, ovariohysterectomy treatment was decided. During laparotomy, one foetus was detected in the cranial portion of the left uterine horn. After manual reduction and intraabdominal withdrawal of the prolapsed uterus, a caesarean section was performed to deliver the foetus. The last litter and the queen were discharged from the clinic in …

Molecular Characterization Of The Nematode Heterakis Gallinarum (Ascaridida: Heterakidae) Infecting Domestic Chickens (Gallus Gallus Domesticus) In Tunisia, Nabil Amor, Sarra Farjallah, Osama Badri Mohammed, Abdulaziz Alagaili, Lilia Bahri

Turkish Journal of Veterinary & Animal Sciences

Heterakis gallinarum is one of the most recurrently diagnosed nematodes within the gastrointestinal tract of galliform birds. In the present study, we investigated the genetic diversity of 96 H. gallinarum collected from free-range chickens from different localities in Tunisia. We assessed phylogeny and genetic variability using the internal transcribed spacers (ITS) of the nuclear ribosomal DNA and the mitochondrial cytochrome c oxidase subunit I gene. Haplotype and nucleotide diversities indicate that H. gallinarum is a species with extremely low genetic diversity. Based on the networks and phylogenetic trees, there was strong support for the absence of significant geographical structuring among …

Feeding Value Of Rice Gluten Meal As An Alternate Protein Source In Broiler Chickens, Manzoor A. Wani, Pramod Kumar Tyagi, Nasir Akbar Mir, Rupjyoti Hazarika, Sajad A. Sheikh, Praveen Kumar Tyagi, O. P. Dinani, Asit Baran Mandal

Turkish Journal of Veterinary & Animal Sciences

he objective of this study was to investigate the effect of feeding rice gluten meal (RGM) on broiler chicken performance, nutrient utilization, intestine morphometry, and cost economics of broiler chicken production. Two hundred forty day-old CARIBRO-vishal variety of broiler chickens were randomly divided into six treatment groups having five replicates with eight birds in each. The formulated diets, containing different levels of RGM (0.0%, 5.0%, 7.5%, 10.0%, 12.5%, and 15.0%) as soybean replacement, were fed to birds for 42 days. In general, the results revealed no significant effect of RGM feeding on average daily gain and feed conversion ratio, but …

Isolation Of Arcobacter Spp. From Domestic Ducks And Geese And Identification Of The Recovered Isolates By Using Molecular Method, Eli̇f Çeli̇k, Ali̇ye Gülmez Sağlam, Özgür Çelebi̇, Sali̇h Otlu

Turkish Journal of Veterinary & Animal Sciences

This study aimed to investigate the role of healthy domestic geese and ducks as Arcobacter carriers. A total of 599 samples, including 330 cloacal swab and 116 fecal samples from geese, and 153 cloacal swab samples from ducks raised on family farms were analysed. For this purpose, the membrane filtration method was applied. In this study, Arcobacter spp. were isolated at rates of 16.7% and 12.93% from cloacal swab and stool samples of geese, respectively, and 26.14% from cloacal swab samples of ducks. Obtained isolates were identified by multiplex PCR (m-PCR) as A. cryaerophilus, A. butzleri, and A. skirrowii. Seasonal …

Vibrio Parahaemolyticus Atcc 17802 Inactivation By Using Methylcellulose Films Containing Encapsulated Bacteriophages, Seli̇n Kalkan

Turkish Journal of Veterinary & Animal Sciences

In the present study, it was aimed to inactivate Vibrio parahaemolyticus ATCC 17802, which is an important human pathogen, by making use of edible methylcellulose films containing encapsulated bacteriophages. For this purpose, the bacteriophages used as biocontrol agents specific for V. parahaemolyticus ATCC 17802 were isolated from raw bonito fishes (Sarda sarda) and then purified, and finally the stabilities of isolated bacteriophages were determined. The pure bacteriophage filtrates were encapsulated with sodium alginate by employing an extrusion method and the edible methylcellulose films were prepared. The bacteriophage stabilities of the prepared films were analyzed on the 0th, 4th, 7th, and …

The First Case Of Traumatic Myiasis Caused By Musca Domestica In A Dog In Konya, Turkey, Bi̇lal Di̇k, Ceylan İlhan, Onur Ceylan, Elgi̇n Orçum Uzunlu

Turkish Journal of Veterinary & Animal Sciences

A case of traumatic myiasis caused by the Musca domestica (Linnaeus 1758) housefly was detected in a dog in Konya, Turkey. A 4-year-old Kangal breed dog injured in a traffic accident was brought to the surgery clinic of the Faculty of Veterinary Medicine at Selçuk University in Konya, Turkey for treatment. Several dipterous larvae were collected from the wound of the dog, placed in a petri dish with 70% ethanol, and sent to the parasitology laboratory for identification. The larvae were examined macroscopically. The specimens were identified as third-instar larvae of M. domestica. This is the first report of traumatic …

Nonsurgical Treatment Involving A Contact Lens And Hyperosmotic Solution For Acute Bullous Keratopathy In A Cat, Sukjong Yoo, Saejong Yoo, Hwi Yool Kim

Turkish Journal of Veterinary & Animal Sciences

A 4-year-old castrated male Korean domestic shorthair cat was referred with bilateral corneal edema. Focal and generalized acute bullous keratopathy in the oculus dexter and oculus sinister, respectively, were diagnosed by ophthalmologic examinations. Intensive topical treatment was administered to both eyes. A therapeutic soft contact lens was inserted in the oculus sinister as a corneal bandage instead of surgical treatment. The patient achieved complete recovery at 44 days after treatment. A therapeutic soft contact lens and an intensive regimen involving a topical hyperosmotic solution successfully treated acute bullous keratopathy in a feline patient.

Feeding Dynamics And Performance Of Beetal Does In Relation To Body Condition Score At The Time Of Mating, Arpan Sharma, Sandeep Kaswan, S. Siva Kumar, Jaspal Singh Lamba

Turkish Journal of Veterinary & Animal Sciences

The body condition of does at the time of mating may affect feeding patterns and production performance during forthcoming lactation. For investigation, 40 Beetal does available for breeding were utilized, and their body condition score (BCS) was estimated using the visual-cum-palpation method with a 9-point scale (scale of 1=5 with increments of 0.5 point). The available goats were divided into 3 groups: higher BCS (T$_{≥3.0}$; n =11), moderate BCS (T$_{2.5}$; n = 8), and lower BCS (T$_{≤2.0}$; n = 21) and maintained in 2 random groups (20 each). Beetal goats with higher BCS at breeding time spent significantly (P < 0.01) more time feeding comfortably and less time feeding with difficulty than moderate and lower BCS goats. T2.5 and T$_{≤2.0}$ goats struggled (displacement and pushing) more at feeders, which included T$_{≤2.0}$ goats eating from the ground for more time (P < 0.01) than the other groups. T$_{≥3.0}$ goats had more agonistic interactions (threatening and hitting) toward moderate and lower BCS goats at feeders. Goats with higher BCS at mating had significantly (P < 0.05) higher milk yield during subsequent lactation; however, the composition of the milk did not differ among the groups. Mean disease incidence rate and number of days of disease per goat were significantly (P < 0.01) lesser in the T$_{≥3.0}$ group than other groups. It was concluded that Beetal goats should not have less than 3.0 BCS at the time of mating for better performance and health status.

Morphological Diagnosis Of Johne?S Disease: A Case Report In Cattle, Siddharth Gautam, Chitra Joshi, Amol Gurav, Chandrakanta Jana, Anil Kumar Sharma

Turkish Journal of Veterinary & Animal Sciences

The present case report deals with the morphological (gross and histopathological) and histochemical characterization of Johne?s disease in a cow. The carcass was presented in a hidebound condition with sunken eyes and with a history of persistent and nonresponsive diarrhea. Necropsy revealed generalized gelatinization of the visceral and peripheral fat. Thorough gross examination unveiled major pathological changes in the digestive tract. The gallbladder was distended, with severely thickened walls and blood mixed with bile. The small intestine was severely congested and the mesenteric lymph nodes were enlarged. The opening of the small intestine showed marked thickening of the intestinal walls, …

The First-Time Isolation Of Brucella Canis From Two Aborted Bitches In A Kennel In Turkey, Ahmet Murat Sayteki̇n, Mustafa Sencer Karagül, Buket Altuntaş, Gülseren Yildiz Öz, Gülnaz Meci̇toğlu, Özge Yilmaz

Turkish Journal of Veterinary & Animal Sciences

Brucella canis is defined as the specific agent that is responsible for canine brucellosis. In this study, B. canis was isolated and identified from the clinical materials belonging to 2 dogs kept in a breeding kennel in Turkey and brought to the researchers? clinic with abortion complaints. With this study, for the first time ever, B. canis isolation and identification was made from dogs that aborted in a kennel. This study emphasizes

Evaluation Of A Field-Deployable Reverse Transcription-Insulated Isothermal Pcr For Rapid And Sensitive On-Site Detection Of Zika Virus, Mariano Carossino, Yanqiu Li, Pei-Yu A. Lee, Chuan-Fu Tsai, Pin-Hsing Chou, Dennis Williams, Ashley Skillman, R. Frank Cook, Grayson Brown, Hsiao-Fen G. Chang, Hwa-Tang T. Wang, Udeni B. R. Balasuriya

Maxwell H. Gluck Equine Research Center Faculty Publications

Background: The recent emergence of Zika virus (ZIKV) in Brazil and its precipitous expansion throughout the Americas has highlighted the urgent need for a rapid and reliable on-site diagnostic assay suitable for viral detection. Such point-of-need (PON), low-cost diagnostics are essential for ZIKV control in vulnerable areas with limited resources.

Methods: We developed and evaluated a ZIKV-specific field-deployable RT-iiPCR reagent set targeting the E gene for rapid detection of ZIKV in ZIKV-spiked human and mosquito specimens, and compared its performance to the Center for Disease Control and Prevention (CDC) and Pan American Health Organization (PAHO) RT-qPCR assays targeting the E …

An In-Depth Look At Stall Contamination: A Total Of 28 Substances Were Identified In Charles Town Ship-In Stalls As A Mix Of Human Medications And Recreational Substances With Some Actual Equine Medications, Clara Fenger, Maria Catignani, Jake Machin, Thomas Tobin

Maxwell H. Gluck Equine Research Center Faculty Publications

No abstract provided.