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Veterinary Medicine

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Articles 2821 - 2850 of 9770

Full-Text Articles in Medicine and Health Sciences

Effects Of Various Toxeat$^{®}$ Concentrations On Growth Performance, Immune Response, Cecal Microflora, And Gut Morphology In Broilers Fed With Aflatoxin Contaminated Diets, Soheila Sarrami, Parvaneh Jafari, Maryam Tajabadi Ebrahimi, Seyed Naser Mousavi, Seyed Abdollah Hosseini Jan 2019

Effects Of Various Toxeat$^{®}$ Concentrations On Growth Performance, Immune Response, Cecal Microflora, And Gut Morphology In Broilers Fed With Aflatoxin Contaminated Diets, Soheila Sarrami, Parvaneh Jafari, Maryam Tajabadi Ebrahimi, Seyed Naser Mousavi, Seyed Abdollah Hosseini

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to investigate the effects of Toxeat$^{®}$, a mycotoxin binder, on functional indices, immune response, intestinal microflora, and gut morphology in broiler chickens fed aflatoxin-contaminated diets. Three hundred Cobb 500 broilers were randomly divided into 6 treatments with 5 replicates, each with 10 birds. The treatments included T1: negative control (NC), T$_{2:}$ T1 + 1 g/ton aflatoxin (positive control: PC), T$_{3:}$ PC + 1 kg/ton Toxeat$^{®}$, T$_{4:}$ PC + 2 kg/ton Toxeat$^{®}$, T$_{5:}$ PC + 3 kg/ton Toxeat$^{®}$, T$_{6:}$ PC + 1 kg/ton Toxeat$^{®}$ without hydrated sodium calcium aluminosilicate. Growth performance, immune system response, and lactic acid …

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Genetic Appraisal Of Growth Traits In Iranian Native Ghezel Sheep Using Random Regression Models, Bashir Ghahri, Sadegh Alijani, Seyed Abbas Rafat, Reza Nabavi, Hossein Daghigh Kia Jan 2019

Genetic Appraisal Of Growth Traits In Iranian Native Ghezel Sheep Using Random Regression Models, Bashir Ghahri, Sadegh Alijani, Seyed Abbas Rafat, Reza Nabavi, Hossein Daghigh Kia

Turkish Journal of Veterinary & Animal Sciences

This research was carried out to estimate the genetic parameters of the body weight traits of the Ghezel sheep using three different random regression models. The used data were 39,288 test-day records collected from 13,378 animals at Ghezel Sheep Breeding Station in Miandoab, West Azerbaijan Province, Iran from 1994 to 2012. Fixed effects in models included type of birth (single, twin) and year of birth (1994-2012). The weight as a function of age in the days of weighting was considered as the fixed regression of orthogonal polynomial. The random effects of the basic model were direct genetic and direct permanent …

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Quality Characteristics And Fatty Acid Profiles Of Bafra, Akkaraman, Andbafra × Akkaraman F$_{1}$ Lamb Meat, Büşra Yaranoğlu, Ceyhan Özbeyaz Jan 2019

Quality Characteristics And Fatty Acid Profiles Of Bafra, Akkaraman, Andbafra × Akkaraman F$_{1}$ Lamb Meat, Büşra Yaranoğlu, Ceyhan Özbeyaz

Turkish Journal of Veterinary & Animal Sciences

The meat quality of Akkaraman, Bafra, and Bafra × Akkaraman F$_{1}$ (BAF$_{1}$) lamb genotypes was determined and then compared at slaughter weights of 34 and 42 kg. At the beginning of the study, 36 male lambs were fed intensively after weaning at approximately 3 months of age. Six animals of each genotype were slaughtered at each slaughter weight and certain meat quality characteristics, namely meat color, pH, cooking loss, tenderness, water holding capacity, and fatty acid profile, were investigated. The pH values of the M. longissimus dorsi (MLD) for the Bafra, Akkaraman, and BAF$_{1}$ genotypes at 24 h after slaughter …

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Evaluation Of The Attitudes Of Veterinary Students Towards Cancer In Animals Using A Cancer Attitude Scale For Animals, İbrahi̇m Kiliç, İbrahi̇m Demi̇rkan, Aysun Çevi̇k Demi̇rkan, Si̇nan Saraçli Jan 2019

Evaluation Of The Attitudes Of Veterinary Students Towards Cancer In Animals Using A Cancer Attitude Scale For Animals, İbrahi̇m Kiliç, İbrahi̇m Demi̇rkan, Aysun Çevi̇k Demi̇rkan, Si̇nan Saraçli

Turkish Journal of Veterinary & Animal Sciences

The aim of this descriptive study was to examine the attitudes of veterinary undergraduate students towards cancer in animals with cognitive, affective, and behavioral dimensions by a new cancer attitude scale for animals approach. Questionnaires, as data collection tools, were applied to 205 students training to be veterinary surgeons at Afyon Kocatepe University, Faculty of Veterinary Medicine, who were selected by systematic random sampling method. Validity-reliability analysis and t-test as well as ANOVA were used in addition to descriptive statistics for the data analysis. The students? general attitudes to cancer were (X = 3.65) greater than the midscore, 3, according …

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Determination Of The Causal Relationship Between Beef Prices And The Consumer Price Index In Turkey, Ahmet Cumhur Akin, Mustafa Bahadir Çevri̇mli̇, Mehmet Saltuk Arikan, Mustafa Agah Teki̇ndal Jan 2019

Determination Of The Causal Relationship Between Beef Prices And The Consumer Price Index In Turkey, Ahmet Cumhur Akin, Mustafa Bahadir Çevri̇mli̇, Mehmet Saltuk Arikan, Mustafa Agah Teki̇ndal

Turkish Journal of Veterinary & Animal Sciences

The rising prices of beef meat have recently restricted its accessibility for consumers who need to consume it for a balanced and healthy diet. Speculations have been made about the increase in beef meat prices being one of the major factors underlying the increase in the consumer price index (CPI). In this scope, the causal relationship between the monthly average prices of beef and the rate of year-on-year change in the CPI for the period between January 2005 and February 2018 was analyzed in the study. Since time series data were used, the augmented Dickey?Fuller and Phillips-Perron tests were conducted …

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In Vitro Effect Of Recombinant Human Gonadotropins On Meiotic Competence Of Dog Oocytes, Kamber Demi̇r, Mi̇that Evecen, Ramazan Arici, Seli̇n Yağcioğlu, Nur Ersoy, Ni̇lhan Coşkun, Hatem Atalla, Kemal Ak, Sema Bi̇rler, Serhat Pabuccuoğlu Jan 2019

In Vitro Effect Of Recombinant Human Gonadotropins On Meiotic Competence Of Dog Oocytes, Kamber Demi̇r, Mi̇that Evecen, Ramazan Arici, Seli̇n Yağcioğlu, Nur Ersoy, Ni̇lhan Coşkun, Hatem Atalla, Kemal Ak, Sema Bi̇rler, Serhat Pabuccuoğlu

Turkish Journal of Veterinary & Animal Sciences

The reproductive biology of the domestic dog is unique among mammalian species; because of this, in vitro maturation (IVM) rate is still very low compared with other domestic animals in spite of attempts at improvement. The aim of this study was to consider the use of recombinant human gonadotropins as a replacement for pituitary gonadotropins for IVM of dog oocytes. A total of 845 cumulus-oocyte complexes were used in this study. To determine the effects of human recombinant gonadotropins, maturation medium was supplemented with two different concentrations (0.05 or 0.1 IU/mL) of pituitary (pFSH, pLH) and human recombinant (rhFSH and …

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The Coexistence Of Heart Murmurs And Arrhythmias In An Equine Hospital Population ? A Retrospective Study, Ali̇ Cesur Onmaz, Vehbi̇ Güneş, Alexandra Pavaloiu, René Van Den Hoven Jan 2019

The Coexistence Of Heart Murmurs And Arrhythmias In An Equine Hospital Population ? A Retrospective Study, Ali̇ Cesur Onmaz, Vehbi̇ Güneş, Alexandra Pavaloiu, René Van Den Hoven

Turkish Journal of Veterinary & Animal Sciences

This study was performed to characterize the coexistence of valvar insufficiencies of the mitral, tricuspid, aortic, and pulmonary valves and their possible association with atrial fibrillation in an equine clinic population. During an 11-year period, 348 horses had been examined for cardiac arrhythmias and suspected defects of the cardiac valves at the Vetmeduni Vienna, Austria. The study population included 256 male and 92 female horses. A subpopulation of 197 (57%) horses was identified with at least one valvular disorder. In these horses, mitral valve regurgitation (MR) was reported in 124 animals (63%), aortic valve regurgitation (AR) in 101 (51%), tricuspid …

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Comparative Detection Of Bovine Herpesvirus-1 Using Antigen Elisa, Immunohistochemistry And Immunofluorescence Methods In Cattle With Pneumonia, Seli̇m Çomakli, Yavuz Seli̇m Sağlam, Mehmet Özkan Ti̇murkan Jan 2019

Comparative Detection Of Bovine Herpesvirus-1 Using Antigen Elisa, Immunohistochemistry And Immunofluorescence Methods In Cattle With Pneumonia, Seli̇m Çomakli, Yavuz Seli̇m Sağlam, Mehmet Özkan Ti̇murkan

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to detect the prevalence and presence of bovine herpesvirus-1 (BoHV-1) using immunohistochemistry (IHC), immunofluorescence (IF) and antigen enzyme-linked immunosorbent assay (ELISA) in the lung samples of cattle (n = 1023). In addition, three methods were compared using receiver operating characteristic (ROC) and chi-square test ($χ^{2}$ test), and their usability were evaluated in laboratory conditions. Macroscopically, pneumonia was seen in 120 of the lung tissue samples (11.73%). Based on the microscopic examinations, the pneumonia types were classified as catarrhal-suppurative bronchopneumonia (6.7%), fibrinous bronchopneumonia (5%), interstitial pneumonia (84.1%), and granulomatous pneumonia (4.2%). The IHC, IF and antigen ELISA …

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Productive Response To Two Concentrate Allocation Strategies In Dairy Cows Grazing With Restricted Pasture Allowance, Gustavo Bretschneider, Eloy Salado, Alejandra Cuatrin, Juan Mattera, Mariela Pece, Dario Arias, Maira Cuffia, Virginia Dieguez Jan 2019

Productive Response To Two Concentrate Allocation Strategies In Dairy Cows Grazing With Restricted Pasture Allowance, Gustavo Bretschneider, Eloy Salado, Alejandra Cuatrin, Juan Mattera, Mariela Pece, Dario Arias, Maira Cuffia, Virginia Dieguez

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to compare the effects of two concentrate allotment strategies on the performance of dairy cows grazing on restricted pasture and supplemented with conserved forage. Dairy cows fed equal amounts of concentrate (fixed daily rate: 5.5 kg DM/cow) were compared to cows supplemented with concentrate according to their lactation stage as follows: 10.0, 5.0, and 1.5 kg DM/cow daily during early, mid, and late lactation, respectively (average daily dose: 5.5 kg DM/cow). There was a supplementation strategy × lactation stage interaction (P < 0.05) for energy-corrected milk (ECM). For early lactation cows, supplementation at a variable rate increased ECM yield by 14.61% (P < 0.05). There was a supplementation strategy × lactation stage interaction (P < 0.05) for body weight change. At late lactation stage, cows supplemented at a fixed rate gained weight (+ 0.16 kg/day), whereas those supplemented at a variable rate lost weight (- 0.08 kg/day; P < 0.05). No significant effect was found for body condition score. The prioritization of milk production rather than the attenuation of body reserve mobilization suggests that concentrate allowance based on lactation stage may be unsuitable for minimizing the negative energy balance in early lactation cows.

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Hematologic, Biochemical, Genetic, And Histological Biomarkers For The Evaluation Of The Toxic Effects Of Fipronil For Rhamdia Quelen, Ana Carolina Fredianelli, Victor Hugo Pierin, Samara Caroline Uhlig, Luciana Do Amaral Gurgel Galeb, Daniel Carlos Coatti Rocha, Deivid Roni Ribeiro, Amanda Anater, Claudia Turra Pimpão Jan 2019

Hematologic, Biochemical, Genetic, And Histological Biomarkers For The Evaluation Of The Toxic Effects Of Fipronil For Rhamdia Quelen, Ana Carolina Fredianelli, Victor Hugo Pierin, Samara Caroline Uhlig, Luciana Do Amaral Gurgel Galeb, Daniel Carlos Coatti Rocha, Deivid Roni Ribeiro, Amanda Anater, Claudia Turra Pimpão

Turkish Journal of Veterinary & Animal Sciences

This study aimed to determine the lethal concentration 50% (LC$_{50}$) and behavioral, hematological, biochemical, histopathological, and genetic disorders of fipronil for Rhamdia quelen in acute toxicity tests for 96 h. To determine the LC$_{50}$, 42 juveniles were distributed in groups of 0, 0.1, 0.2, 0.3, 0.4, 0.5, and 0.8 mg L$^{-1}$ of fipronil. For other evaluations 36 silver catfish were divided into control, 0.3 mg L$^{-1}$, and 0.4 mg L$^{-1}$ groups. Blood, kidneys, livers, and gills were collected for evaluation. The LC$_{50}$ calculated with probit-type regression was 0.811 mg L$^{-1}$ at 48 h of study. There was total mortality of …

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Microsatellite Panels For Parentage Testing Of Kilis Goats Reared In Turkey, Mahmut Keski̇n, Onur Yilmaz, Zühal Gündüz, Nezi̇h Ata, Sabri̇ Gül, İbrahi̇m Cemal, Orhan Karaca, Süleyman Ercüment Önel Jan 2019

Microsatellite Panels For Parentage Testing Of Kilis Goats Reared In Turkey, Mahmut Keski̇n, Onur Yilmaz, Zühal Gündüz, Nezi̇h Ata, Sabri̇ Gül, İbrahi̇m Cemal, Orhan Karaca, Süleyman Ercüment Önel

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to develop PCR-based suitable microsatellite marker panels for paternity testing and to define pedigree errors in Kilis goats. A total of 137 head of goats were used, consisting of 118 head of kids and 19 head of possible candidate sires. A total of 392 alleles were observed in 22 microsatellite markers. Allele numbers ranged between 12 (SRCRSP7) and 24 (BM1818, INRA0023, and SRCRSP15) with an average of 17.82. The mean value of the effective allele numbers was obtained as 9.44. The overall polymorphic information content value was quite high (0.88). The overall observed (Ho) …

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Phylogenetic Relationships Of Native Turkish Cattle Breeds Using Microsatellite Markers, Yusuf Özşensoy, Ercan Kurar, Müge Doğan, Zafer Bulut, Mehmet Ni̇zamlioğlu, Ayşe Işik, Aysun Çamlidağ, Vahdetti̇n Altunok Jan 2019

Phylogenetic Relationships Of Native Turkish Cattle Breeds Using Microsatellite Markers, Yusuf Özşensoy, Ercan Kurar, Müge Doğan, Zafer Bulut, Mehmet Ni̇zamlioğlu, Ayşe Işik, Aysun Çamlidağ, Vahdetti̇n Altunok

Turkish Journal of Veterinary & Animal Sciences

A total of 20 microsatellite DNA markers were used for genetic characterization and determination of phylogenetic relationships of native cattle breeds of Turkey, including the Anatolian Grey (AG), Anatolian Black (AB), South Anatolian Red (SAR), East Anatolian Red (EAR), Southern Anatolian Yellow (SAY), and Zavot (ZAV). DNA samples were isolated from 271 blood samples using an organic method. Amplified polymerase chain reaction products were separated by capillary electrophoresis and genotypes were determined for 20 microsatellites. A total of 269 different alleles were determined. The lowest (7.80) and highest (10.80) mean allele numbers were observed for the ZAV and SAY populations, …

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A Case Of Ex Vivo Boosted Immune Cell Therapy For Chronic Ulcerative Dermatitis In A Dog, Seulgi Bae Jan 2019

A Case Of Ex Vivo Boosted Immune Cell Therapy For Chronic Ulcerative Dermatitis In A Dog, Seulgi Bae

Turkish Journal of Veterinary & Animal Sciences

A 13-year-old castrated male Shih Tzu was presented with recurrent otitis externa and ulcerative dermatitis. Previous investigations had failed to identify the cause, and the lesion was resistant to treatment. According to the histopathological examination, a tentative diagnosis of chronic ulcerative dermatitis with bacterial infection was made. To treat the patient, immune cell therapy with antibiotics was conducted every 2 weeks, for a total of six sessions. After treatment, the skin lesion resolved. No side effects were observed during therapy. To the best of our knowledge, this is the first case report of the use of immune cell therapy for …

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Influence Of Rearing Conditions And Birth Season On Calf Welfare In The First Month Of Life, Ljiljana Samolovac, Slavca Hristov, Branislav Stankovic, Radojka Maletic, Renata Relic, Zvonko Zlatanovic Jan 2019

Influence Of Rearing Conditions And Birth Season On Calf Welfare In The First Month Of Life, Ljiljana Samolovac, Slavca Hristov, Branislav Stankovic, Radojka Maletic, Renata Relic, Zvonko Zlatanovic

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to analyze the influence of farm conditions and season of birth on body mass, respiratory system diseases and diarrhea occurrence, and mortality during the first month of calves? life. The study was conducted on two tie-stall-system farms with intensive milk production and similar nutrition of dairy cows, but with differences in rearing conditions of calves in the first week of life. The calves were tied on lying area with straw as bedding material (farm A) or free in individual boxes with straw bedding (farm B). In the first consumption, they take 1-2 L of …

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The Influence Of Haptoglobin Phenotype On Differential Leukocyte Count In Neonatal Calves, Elena Kalaeva, Olga Zemlyanukhina, Vladislav Kalaev, Kseniya Efimova, Anton Chernitskiy, Nikolay Kaverin, Vladimir Safonov Jan 2019

The Influence Of Haptoglobin Phenotype On Differential Leukocyte Count In Neonatal Calves, Elena Kalaeva, Olga Zemlyanukhina, Vladislav Kalaev, Kseniya Efimova, Anton Chernitskiy, Nikolay Kaverin, Vladimir Safonov

Turkish Journal of Veterinary & Animal Sciences

This research was conducted to study the content and phenotypes of haptoglobin and their correlation to the differential leukocyte count in healthy Red-motley Holstein calves (n = 30). The blood samples from calves were taken through puncture of the jugular vein on days 1, 7, 14, and 28 after birth. Differential leukocyte count was analyzed by manual enumeration. Serum haptoglobin content was determined by spectrophotometry (UV-1700 Shimadzu, Japan). Haptoglobin phenotypes were studied using gel electrophoresis. Serum haptoglobin content in calves on day 1 after birth was 3.8 ± 0.7 g/L, on day 7 was 3.5 ± 0.7 g/L, and on …

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Sequence Characterization Of Full-Length S, Yeganeh Yousefi, Mohammad Reza Bassami, Gholam Ali Kalidari, Mohammad Mehdi Ghahramani Seno Jan 2019

Sequence Characterization Of Full-Length S, Yeganeh Yousefi, Mohammad Reza Bassami, Gholam Ali Kalidari, Mohammad Mehdi Ghahramani Seno

Turkish Journal of Veterinary & Animal Sciences

Infectious bronchitis virus (IBV) causes heavy economic losses in chicken farms worldwide. Like many other viral diseases, vaccination is the gold standard approach in controlling and preventing the spread of IBV. However, IBV is continuously evolving and new strains of this virus emerge that, due to variable antigenic cross-reactivity, may reduce or nullify the protective effect of vaccination programs. Therefore, advance knowledge of the IBV strain(s) circulating in a region will be very useful in deciding which commercial vaccine(s) would be most effective. In this line, we analyzed the nucleotide sequence of the full-length 1$_{1}$ gene of IBV viruses isolated …

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Management Of A Coxiella Burnetii-Infected Sheep Flock After An Outbreak Of Q Fever In Humans, Jana Avbersek, Mateja Pate, Andrej Skibin, Matjaz Ocepek, Brane Krt Jan 2019

Management Of A Coxiella Burnetii-Infected Sheep Flock After An Outbreak Of Q Fever In Humans, Jana Avbersek, Mateja Pate, Andrej Skibin, Matjaz Ocepek, Brane Krt

Turkish Journal of Veterinary & Animal Sciences

Following an outbreak of Q fever in a group of students who contracted the infection during a training course on a sheep farm, a detailed investigation of the sheep flock involved was conducted. Of 478 flock animals, 60 Coxiella burnetii ELISA-positive and 60 ELISA-negative ewes were selected for the trial and divided into four groups. A month after the initial ELISA screening, all ewes in the flock (except the control group) were vaccinated. Sequentially collected blood samples were tested with ELISA and PCR; feces, milk, manure, bedding, and soil were tested with PCR. The immune response to the vaccination was …

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Negative Effects Of Occurrence Of Clinical Mastitis From Calving To End Of The Voluntary Waiting Period On Reproduction In Holstein Cows, Seçki̇n Salar, Oğuz Çalişici, Duygu Çalişici, Doğukan Özen, Ayhan Baştan Jan 2019

Negative Effects Of Occurrence Of Clinical Mastitis From Calving To End Of The Voluntary Waiting Period On Reproduction In Holstein Cows, Seçki̇n Salar, Oğuz Çalişici, Duygu Çalişici, Doğukan Özen, Ayhan Baştan

Turkish Journal of Veterinary & Animal Sciences

The objective was to evaluate the effects of clinical mastitis (CM) on reproductive performance in high-milk?producing cows before the service period. The data of Holstein Friesian cows (n = 550) in a commercial farm was retrospectively evaluated. The diagnosis of CM was made by the presence of visible abnormalities in milk or symptoms of inflammation of one or more of the quarters. Cows were checked to detect CM prior to each milking. Calving to first service interval (CFSI), calving to conception interval (CCI), and services per conception (SC) were evaluated as reproductive parameters. Calving to first service interval (83.68 ± …

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Formation Of Local Protection Of The Respiratory Tract In Holstein Calves, Yuriy Alekhin, Maksim Zhukov, Valentina Morgunova, Galina Chusova, Irina Klementeva Jan 2019

Formation Of Local Protection Of The Respiratory Tract In Holstein Calves, Yuriy Alekhin, Maksim Zhukov, Valentina Morgunova, Galina Chusova, Irina Klementeva

Turkish Journal of Veterinary & Animal Sciences

The processes of formation of the local protection of the respiratory tract in nasal secretion samples of Holstein calves from birth to 6 months of age that were born from clinically healthy cows were examined. Only clinically healthy animals were involved in the experiment. Nasal secretion samples were collected with the help of a sterile foam tampon with a fixing thread in order to assess local immunity in animals at the age of 1, 5, 10, 30, 60, 90, 120, 150, and 180 days. Total protein, mucin, lysozyme activity, and alkaline phosphatase (ALP) were determined using commercially available methods. The …

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The Effect Of Laying Period On Egg Quality Traits And Chemical Composition Of Lindovskaya (Linda) Geese Reared Under Breeder Conditions, Mehmet Sari, Kadi̇r Emre Buğdayci, Aykut Asim Akbaş, Mustafa Saatci, Mustafa Numan Oğuz Jan 2019

The Effect Of Laying Period On Egg Quality Traits And Chemical Composition Of Lindovskaya (Linda) Geese Reared Under Breeder Conditions, Mehmet Sari, Kadi̇r Emre Buğdayci, Aykut Asim Akbaş, Mustafa Saatci, Mustafa Numan Oğuz

Turkish Journal of Veterinary & Animal Sciences

The study was conducted to determine the effect of laying period on egg quality traits as well as the chemical composition of Lindovskaya geese. The egg quality traits were examined by randomly taking a total of 90 eggs on the 45th ± 5, 60th ± 5, and 75th ± 5 days of the laying period from 3-year-old female Lindovskaya geese. It was determined that the effect of the laying period on external quality traits of eggs was statistically nonsignificant (P > 0.05). The effect of laying period on internal quality traits including height, diameter, and index of the yolk and albumen …

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Neutralizing Antibody Titers Against Field Strains Of Bovine Viral Diarrhea Virus After Vaccination With Three Commercial Vaccines, Gi̇zem Alpay, Kadi̇r Yeşi̇lbağ Jan 2019

Neutralizing Antibody Titers Against Field Strains Of Bovine Viral Diarrhea Virus After Vaccination With Three Commercial Vaccines, Gi̇zem Alpay, Kadi̇r Yeşi̇lbağ

Turkish Journal of Veterinary & Animal Sciences

Modified killed vaccines against bovine viral diarrhea virus (BVDV) are used worldwide. In the present study, the cross-neutralization antibody responses against field strains from seven subgenotypes of BVDV-1 and one subgenotype of BVDV-2 were evaluated using sera obtained by three inactivated commercial vaccines. One vaccine contained both BVDV-1 and BVDV-2 strains, while the others contained only BVDV-1a. Three vaccine groups, each containing five calves, were vaccinated two times with 30-day intervals. The antibody titers were evaluated by virus neutralization assay. The monovalent vaccine induced the highest antibody titers. Significant levels of neutralizing antibody titers were maintained up to the last …

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Histomorphological And Histochemical Structure Of The Midgut And Hindgut Of The Caucasian Honey Bee (Apis Mellifera Caucasia), Ahmet Ceylan, Sedat Sevi̇n, Özge Özgenç Jan 2019

Histomorphological And Histochemical Structure Of The Midgut And Hindgut Of The Caucasian Honey Bee (Apis Mellifera Caucasia), Ahmet Ceylan, Sedat Sevi̇n, Özge Özgenç

Turkish Journal of Veterinary & Animal Sciences

The Caucasian honey bee (Apis mellifera caucasia subspecies of Apis mellifera L.) is one of the most common and prominent honey bee breeds in Turkey. The morphometry of endemic honey bee breeds has been extensively studied, but little attention has been given to the microscopic morphology of these bees. The aim of the current study was to describe for the first time the histomorphology of the midgut and hindgut of the Caucasian honey bee in Turkey. A total of 20 local Caucasian adult honey bee workers were sampled for histomorphological and histochemical analysis. The midgut epithelium consisted of epithelial cells …

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Development Of Spermatogonial Stem Cell Niche And Immunoexpression Of Vimentin Filaments In The Testes Of Prenatal And Postnatal Indian Buffalo (Bubalus Bubalis), Ashritha Quanisha Carlo, Devendra Pathak, Opinder Singh, Neelam Bansal Jan 2019

Development Of Spermatogonial Stem Cell Niche And Immunoexpression Of Vimentin Filaments In The Testes Of Prenatal And Postnatal Indian Buffalo (Bubalus Bubalis), Ashritha Quanisha Carlo, Devendra Pathak, Opinder Singh, Neelam Bansal

Turkish Journal of Veterinary & Animal Sciences

In this study, we established the components of the buffalo spermatogonial stem cell (SSC) niche in prenatal (38.5-280 days of gestation), neonatal, prepubertal, and adult testes histochemically and immunohistochemically. Immunohistochemically, the changes in vimentin expression were evaluated. During prenatal life, the niche of primordial germ cells was formed by the surrounding mesenchymal cells and fibroblasts. The basement membrane (BM) was not organized at this stage. At 7.6 cm crown-rump length, testicular cords with a thin BM were seen. At the late gestational period, testicular cords with well-organized BM were seen. At the neonatal stage, the testicular cords were lined with …

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Phenotypic Characterization And Differentiation Of Mesenchymal Stem Cells Originating From Adipose Tissue, Ece Çerçi̇, Hati̇ce Erdost Jan 2019

Phenotypic Characterization And Differentiation Of Mesenchymal Stem Cells Originating From Adipose Tissue, Ece Çerçi̇, Hati̇ce Erdost

Turkish Journal of Veterinary & Animal Sciences

Stem cells are known as undifferentiated cells that are capable of divisions and self-renewal over an extended period. Adipose tissue-derived mesenchymal stem cells (ADSCs) are known to differentiate into many different organ and tissue cells. The purpose of this study was to characterize adipose tissue-derived mesenchymal stem cells in detail based on the expression status of certain surface markers and differentiation stages for clinical and laboratory applications. The migrating phase and mitotic property of the cells from adipose tissue were assessed under a microscope. Cell counting was performed during the first, second, and third passages. The cells were differentiated into …

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Akt Expression And Lactose Synthesis During Heat Exposure In Saanen Goat, Nungnuch Saipin Jan 2019

Akt Expression And Lactose Synthesis During Heat Exposure In Saanen Goat, Nungnuch Saipin

Chulalongkorn University Theses and Dissertations (Chula ETD)

In tropical countries, high ambient temperature (HTa) is a fundamental factor that cause a decline in the mammary gland function in dairy animals. Dairy animals fed under HTa decreased milk yield (MY) as well as milk compositions. The current study focuses on the effect of HTa to decrease milk synthesis from natural and in vitro HTa condition. The first experiment was performed to investigate the effect of HTa from the natural condition in early lactating Saanen goat, 2-4 weeks of postpartum (2 PP-4 PP). Five animals were investigated during the winter season as control ambient condition. Six animals as the …

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Studies Of Beta-Cardiotoxin From King Cobra Venom On Rat Cardiac Functions: Effect On Isolated Single Cardiomyocyte, Tuchakorn Lertwanakarn Jan 2019

Studies Of Beta-Cardiotoxin From King Cobra Venom On Rat Cardiac Functions: Effect On Isolated Single Cardiomyocyte, Tuchakorn Lertwanakarn

Chulalongkorn University Theses and Dissertations (Chula ETD)

Beta-cardiotoxin (ß-CTX), a novel protein isolated from the King cobra (Ophiophagus hannah) venom has previously been proposed as a beta-blocker candidate. However, cellular mechanisms of ß-CTX on cardiomyocyte is unknown. This study aimed to evaluate the impact of ß-CTX on isolated rat cardiomyocyte function and the cardiac myofibrillar activity, and to explore involvement of ß-adrenergic receptor (ß-AR) signaling pathway. ß-CTX was isolated and purified using two-step chromatographic method and confirmed by N-terminal sequencer. The function of ß-CTX on cardiomyocyte was compared to propranolol in 3 conditions, basal state, with isoproterenol (ISO), and with forskolin (FSK). ß-CTX exhibited more potency than …

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The Left Ventricular Functions And Heart Rate Variability In Dogs With Pulmonic Valvular Stenosis, Ploypanut Trikhun Jan 2019

The Left Ventricular Functions And Heart Rate Variability In Dogs With Pulmonic Valvular Stenosis, Ploypanut Trikhun

Chulalongkorn University Theses and Dissertations (Chula ETD)

Pulmonic valvular stenosis (PS) is one of the most common congenital heart diseases in dogs that can lead to the cardiac maladaptation. Also, PS can affect the cardiac autonomic nervous system (ANS). However, clinical information regarding to systolic function and cardiac ANS alteration in PS dogs had not been fully elucidated. The objectives of this study were to evaluate cardiac electrical property, left ventricular (LV) function, and to assess cardiac ANS from heart rate variability (HRV) analysis in PS dogs compared with healthy dogs. The dogs in this study were divided into 2 groups, PS dogs (n=13) and healthy control …

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Genetic And Pathogenic Characterizations Of Newly Emerged Duck Tembusu Virus Isolated In Thailand, Patchareeporn Ninvilai Jan 2019

Genetic And Pathogenic Characterizations Of Newly Emerged Duck Tembusu Virus Isolated In Thailand, Patchareeporn Ninvilai

Chulalongkorn University Theses and Dissertations (Chula ETD)

Duck Tembusu virus (DTMUV), a mosquito-borne Flavivirus, caused an emerging disease in ducks in Asia since 2010, resulting in significant economic loss in duck producing industry. After the initial outbreak in 2013, DTUMV cases have been continuously detected in several duck farms in Thailand. Thus, the disease surveillance and the genetic characterization of the Thai DTMUV are necessary. Therefore, the first objective of this dissertation was to investigate the genetic characteristic of DTMUVs circulating in Thailand. Our retrospective study demonstrated the presence of DTMUV in the Thai ducks since 2007. Phylogenetic analysis of the polyprotein gene sequence revealed that the …

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Single Fixed-Time Artificial Insemination With Fresh Or Frozen Thawed Semen In Pig, Pachara Pearodwong Jan 2019

Single Fixed-Time Artificial Insemination With Fresh Or Frozen Thawed Semen In Pig, Pachara Pearodwong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Single fixed-time AI is the breeding protocol inseminating the sows once with either fresh or frozen-thawed (FT) semen at fixed time after ovulation induction without estrus detection. To develop the success single fixed-time AI protocol in Thailand, factors affecting the efficacy of single fixed-time AI were evaluated. Additionally, we also evaluated the effect of using lecithin in cryopreservation extender on FT semen qualities in order to find the possibility to perform single fixed-time AI using sperm preserved in lecithin based extender. It was found that administration of 10 ug buserelin at 72 h after weaning decreased weaning-to-ovulation interval compared to …

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Development Of An Indirect Elisa To Detect Antibodies Against Actinobacillus Suis Using Boiled Whole Cell Antigen, Siriluck Juntautsa Jan 2019

Development Of An Indirect Elisa To Detect Antibodies Against Actinobacillus Suis Using Boiled Whole Cell Antigen, Siriluck Juntautsa

Chulalongkorn University Theses and Dissertations (Chula ETD)

Actinobacillus suis is considered an opportunistic pathogen in swine that can cause disease in animals of all ages, especially in high health status herds. The objective of this study was to developed an indirect enzyme-linked immunosorbent assay (ELISA) using boiled whole cell antigen for the detection of antibody against A. suis. The selected herd has clinical problems associated with A. suis infection. In addition, this herd was not received any Actinobacillus pleuropneumoniae (APP) vaccine nor diagnosed with APP infection before the study. The isolation of A. suis was recovered from lung of the infected pig in the selected herd. Moreover, …

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