Animal Experimentation and Research Commons^™

Protection For Animals In Biomedical Research, F. L. Thomsen

Laboratory Experiments Collection

Our conclusion from all of this work and study is that not just a small part, but that most of the suffering undergone by laboratory animals in "unnecessary" under the terms of the pain provisions of the Rogers-Javits bill. Granted, it will take some time and effort to bring about the necessary interpretations of these provisions. The Act, when passed, offers us the medium through which to obtain such interpretations.

This unnecessary suffering results mostly from the indifference, and from the inertia and the lack of proper scientific training and technical knowledge, of many of those conducting laboratory animal experiments …

Pig Husbandry Research In Western Australia, N W. Godfrey

Journal of the Department of Agriculture, Western Australia, Series 4

IN the past it has not been possible to undertake research work with pigs on any appreciable scale in Western Australia.

A small herd has been maintained at Denmark Research Station for some years as a demonstration side-line unit, run in conjunction with the dairy herd.

A number of very useful trials have been carried out with the Denmark herd, but its size has limited the comprehensiveness of the type of research work that could be undertaken.

The Transfer Of Hydrogen In The Reduction Of Progesterone, William H. Kersey

Loma Linda University Electronic Theses, Dissertations & Projects

Experimental female rats were given daily injections of synthetic estrogens for six months. The effect on rat ovarian 20α-OH-SDH was not definitive. Diethylstilbestrol and 17α-ethynylestradiol caused a significant drop in activity as compared with controls while mestranol caused a slight increase in activity. A synthetic progestin, medroxyprogesterone, had no apparent effect on the activity of the enzyme.

Rat ovarian 20α-OH-SDH was purified fivefold by ammonium sulfate fractionation and chromatography on DEAE-cellulose.

The homogeneity of the enzyme was checked by means of disc gel electrophoresis. At pH 8.2 no significant contamination appeared in the electrophoretic pattern, but at pH 9.0 five …

Concurrent Infections Of Schistosoma Mansoni And Trypanosoma Equiperdum In Mice, Venus Ee-Siriporn

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this investigation was to determine factors which may influence the survival time of mice infected with Trypanosoma equiperdum and the effects of T. equiperdum on mice infected with Schistosoma mansoni.

In order to maintain experimentally obtained standard hours of survival (88 hrs) in mice inoculated intraperitoneally with 150,000 T. equiperdum, it was found that trypanosomes removed from the donor rat should be kept at 25 C and all counts, dilutions of the organisms, and injection should be completed within 1 hour after removal from the donor host. Survival time of mice is increased as the …

Craniofacial Changes Resulting From Expansion Of The Midpalatal Suture In The Macaca Mulatta Monkey As Revealed By Roentgenographic Analysis, Raymond M. Sugiyama

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to determine the effects of midpalatal suture separation on the craniofacial complex by quantitative roentgenographic analysis.

Ten female Macaca mulatta monkeys were randomly grouped for experimentation. Six animals were paired in groups for two months of treatment, four months of treatment followed by two months of post retention, and six months of treatment. The four remaining monkeys were paired and served as control animals for two and six month periods. Total activation of the expansion appliances in the treated animals was completed over a three week period.

The findings of this study were based …

Forced Separation And Consequent Stability Of The Midpalatal Suture Of The Macaca Mulatta Monkey, Frederick L. Hoffer Jr.

Loma Linda University Electronic Theses, Dissertations & Projects

This study was designed to (1) demonstrate the dimensional changes resulting from opening the suture, (2) test the stability of the repaired suture two months following the removal of the retentive appliance, and (3) demonstrate new bone growth in the opened suture by the use of Tetracycline staining. The animal chosen was the Macaca mulatta monkey.

Intact skulls, sectioned skulls and dental casts were measured following the experiment. The skulls were mounted in clear plastic and sectioned antero-posteriorly for the purposes of study and measurements. The following changes were noted as a result of this experiment:

1. The floor of the …

Oral Bandage Tissue Reaction In Dogs, John Rozema

Loma Linda University Electronic Theses, Dissertations & Projects

This experiment was designed to evaluate an oral surgical dressing which may be used as an aid to healing tissue. The parameters of evaluation were directed toward wound healing enhancement, duration, and stability of the bandage. A histological evaluation of the surgical wounds protected with the bandage as compared to the control wounds was made.

The surgeries were performed on eight dogs in a double controlled experiment and were in the form of bilateral maxillary gingivectomies and mandibular molar tooth extractions. The surgery sites on the left were covered with the Orahesive Bandage while the right side remained exposed to …

Effects Of Poor Quality Diets With Various Dietary Factors Added And Exposure To Cigarette Smoke On The Voluntary Alcohol Intakes Of Albino Rats, Bonnie Emmerson

Loma Linda University Electronic Theses, Dissertations & Projects

Studies were conducted utilizing albino rats to determine the relationship of voluntary alcohol consumption with various dietary additives and exposure to cigarette smoke in combination with poor quality diets. Male, Sprague-Dawley rats, from weanling age to 100 grams, were given the various experimental diets and a free fluid choice of ten per cent ethanol or tap water. Fluid intakes were recorded and analyzed statistically. Individual variation was common with a generally low level of alcohol consumption when rats were fed a poor quality diet of common United States foods. The addition of caffeinated beverages and/or spices and relish caused increases …

Periosteal Circulation And Its Contribution To The Nutrition Of Compact Bone, Jean Elaine Tassell

Loma Linda University Electronic Theses, Dissertations & Projects

[Abstract Not Included]

Carbon Dioxide Fixation In Schistosoma Mansoni, Clint Earl Carter

Loma Linda University Electronic Theses, Dissertations & Projects

Adults of the blood fluke Schistosoma mansoni were perfused from the hepatic portal system of CF1 female mice sixty to seventy days post infection. The worms were then incubated in Eagle’s minimal essential medium with glutamate and 0.03 mg/100 ml of nonlabelled aspartic acid. To this medium was added 10 μc of either NaH¹⁴0₃, glucose-UC¹⁴ or aspartate-4-C¹⁴. The worms were found to fix carbon from the NaH¹⁴0₃ and to incorporate the glucose and aspartate. The amount of activity recovered in five different chemical fractions after two hours incubation was determined …

Mandibular And Alveolar Growth In Dog Following Infusion Of 5-Fluorouricil, Richard D. Pfeiffer

Loma Linda University Electronic Theses, Dissertations & Projects

The action of a chemotherapeutic agent as a tool in studying its effect upon a primary growth center, the mandibular condyle, was investigated. So far as can be ascertained, no studies have been reported where these growth sites have been eliminated or modified by the use of chemotherapeutic growth inhibitors.

Observation of mandibular and alveolar growth following the infusion into the left external carotid artery of a cancer chemotherapeutic agent, 5-Fluorouricil (Roach-Hoffman), was noted. Comparisons were made between the infused and the uninfused sides of the mandible observing gross growth changes.

Four dogs, aged 75 days, were infused with varying …

The Effect Of Schistosoma Mansoni On Free Amino Acid Levels In Mice, Richard D. Tkachuck

Loma Linda University Electronic Theses, Dissertations & Projects

Free amino acid levels in the blood plasma of mice infected with Schistosoma mansoni Sambon, 1907, were measured at 3, 6, and 9 weeks post-infection with an automatic amino acid analyzer. Significant differences between experimental and control animals occurred at all three time periods. At 3 weeks, asparagine, alanine, citrulline, proline, methionine, and threonine levels all were lower in the infected animals. At 6 weeks, four differences were noted. Alpha-amino-n-butyric acid, ornithine, lysine, and histidine all had lower values in infected animals. At 9 weeks there were 12 differences. Tyrosine, phenylalanine, threonine, serine, proline, citrulline, glycine, alanine, valine, isoleucine, and …

The "Starvation Effect" And Tryptophan Pyrrolase Activity In The Vermilion Mutant Of Drosophila, Ernest E. Shelton Jr.

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The enzyme tryptophan pyrrolase is controlled by the vermilion locus in Drosophila melanogaster and is normally lacking in the vermilion mutants. During this investigation it has been demonstrated that v¹;bw flies have tryptophan pyrrolase activity when grown under near starvation conditions.

During development of the fly there is a peak of enzyme activity in the early third instar soon after the transfer to starvation medium. A second peak of enzyme activity occurs during the pupal stage. The activity in v¹;bw pupae is about 15% of the activity found in cn bw adults.

In v …

The Fecal Shedding Of Listeria Monocytogenes By Conventional And "Germ Free" Mice, Margaret Joyce Ramsay

Loma Linda University Electronic Theses, Dissertations & Projects

A series of experiments involving Listeria monocytogenes was undertaken to study first the fecal shedding of Listeria by conventional mice challenged intraperitoneally with Listeria and second the fecal shedding and ability to survive of germ free mice with similar challenge. In addition, mites infesting Listeria infected mice were cultured for their possible contamination by Listeria.

Conventional mice were challenged intraperitoneally with a single near LD₅₀ dose of Listeria. Fecal Listeria were detected by collecting specimens from each mouse daily for the first 4 days and every second day thereafter. The feces was held at 7°C for 14 …

How Can We Best Help Laboratory Animals Now?, Oliver Evans

Laws and Legislation Collection

No abstract provided.

Ixodid Ticks: Possible Vectors Of Tuberculosis, Ya. A. Blagodarnyy, I. N. Blekhman, M. P. Yukunin

United States Navy: Publications

"From these tests, we established that artificially and naturally fed ticks are susceptible to the infective source and preserve tuberculosis mycobacteria in the body for a long period."

Antibiotics And The Wasting Disease In Neonatally Thymectomized Rats, Douglas M. Grignon

Loma Linda University Electronic Theses, Dissertations & Projects

The administration of the antibiotic, Chloramphenicol, to pregnant rats just prior to, and for four days after delivery, diminishes the incidence of the so-called "Wasting Disease" in neonatally thymectomized rats.

A total of 50 rats were divided into the following groups:

1. Thymectomy only---nonmedicated 17 rats
2. Thymectomy only---medicated 12 rats
3. Thymectomy-adrenalectomy---nonmedicated 5 rats
4. Thymectomy-adrenalectomy---medicated 8 rats
5. Controls---nonmedicated 4 rats
6. Controls---medicated 4 rats

Chloramphenicol was administered in the drinking water to the mothers of groups 2, 4 and 6 for about 2 days prior to delivery and 4 days after. On the day of birth or within three days after birth, the …

Sheep Breeding Problems In W.A. : The Industry's View, Stanley M. Dennis

Journal of the Department of Agriculture, Western Australia, Series 4

IN 1963 a short questionnaire was distributed to farmers and pastoralists seeking information about sheep breeding problems and lamb losses.

This was a fore-runner to the Department of Agriculture survey into the causes of abortion and lamb mortality which is reported elsewhere in this issue.

How To Identify Twin-Born Lambs, G T. Halpin, P. Nelson

Journal of the Department of Agriculture, Western Australia, Series 4

IN the past few years it has become widely accepted that the ability to produce twin or multiple lambs is a heritable characteristic.

This means that breeding from sheep born as twins should give more multiple births and higher lambing percentages than breeding from sheep born as single lambs.

Kimberley Research Station Progress Report, 1964 : Insect Pests And Insecticides, K T. Richards

Journal of the Department of Agriculture, Western Australia, Series 4

FOR the past four years, detailed studies have been carried out in regard to the identification, status, bionomics, and control of insects and mite pests attacking the various crops grown in the Ord River Irrigation Area.

Dead Lambs : A Dead Loss, Stanley M. Dennis

Journal of the Department of Agriculture, Western Australia, Series 4

During the past year the Animal Health Laboratory has continued its survey into the causes of abortion in sheep and perinatal lamb losses.

This article records the results of this work.

LAMBS and specimens from lambs were submitted to the Animal Health Laboratory from 229 properties situated in 121 areas in this survey during 1964.

Post-mortem examination was carried out on 1,422 lambs and specimens from 61 lambs were also examined.

The Effect Of Varying Protein Intake On Trichinella Spiralis Infection In Mice, Clare K. Kwan

Loma Linda University Electronic Theses, Dissertations & Projects

Three hundred Swiss Webster albino mice three weeks of age were placed on low (8%), medium (24%), high (48%) protein levels and a commercial laboratory animal feed, Purina Chow for various lengths of time: (1) simultaneously with infection, and four weeks thereafter, (2) three weeks before infection and throughout the four-week infection period, and (3) six weeks prior to infection and during the infection period. The protein source was casein. Sixty of the 300 mice used served as noninfected controls, and the other 240 were each infected with 400 washed, suspended Trichinella spiralis larvae obtained from a rat by the …

The Effect Of Mulesing And Tailstripping At Lamb Marking On Subsequent Lamb Growth And Incidence Of Fly Strike, R J. Lightfoot, W. L. Mcgarry

Journal of the Department of Agriculture, Western Australia, Series 4

THE modified mules and tailstrip operation has been shown to be highly effective in reducing the incidence of crutch strike in sheep.

In Western Australia the operation is usually performed on ewe weaners or hoggets immediately after either crutching or shearing.

Pig Research Projects For W.A, W J O Wilkie

Journal of the Department of Agriculture, Western Australia, Series 4

The Medina Pig Research Unit now being developed by the Department of Agriculture will be an important addition to pig research facilities in Australia.

The primary function of the unit will be to serve the needs of the pig industry in Western Australia.

In this article, Animal Husbandry Officer W. J. Wilkie, B.V.Sc, discusses some aspects of research to be carried out.

Multi-Timer For Milking Studies, G W R Scott

Journal of the Department of Agriculture, Western Australia, Series 4

Recent research into milking machine operation has proved that the belief of old-time hand-milkers that fast milking was good milking was soundly based.

In current practice the emphasis is on convenient shed and yard design and systematic routine in handling the cows. This permits a high throughput of cows and a high output of milk per labour unit.

The multi-timer designed and built by Mr. G. W. R. Scott and described in this article will prove most valuable for pinpointing where time is lost.

Flock Testing : Weighing, Sampling And Measurement Procedures For Fleece Measurement, H G. Neil, R. J. Lightfoot

Journal of the Department of Agriculture, Western Australia, Series 4

An outline of shearing-shed procedure for the collection of greasy fleece weight records and sampling of the fleece for detailed measurement by the Department of Agriculture Flock Testing Service for Merino stud breeders.

A Study Of Regeneration Of Peripheral Nerve In The Rat, James C. Jay

Loma Linda University Electronic Theses, Dissertations & Projects

Due to improvements in fixation and embedding techniques a restudy of regenerating peripheral nerve was thought advisable in order to better understand and describe the processes which take place.

Femoral nerves of white Wistar rats were used. The lesions were made by either crushing or severing the nerve. After a period of regeneration, varying from 12 hours to six days, the wound was reopened and the injured portion fixed in situ with 1% osmium tetroxide. The tissues were then removed, dehydrated, embedded and sectioned. The sections were stained and studied with a modified RCA EMU2A electron microscope.

Observations revealed that …

Quantitative Evaluation Of Streptococcal Antibodies By A Serum Inhibition Fluorescent Antibody Tube Method, Charles Glenn Nichols

Loma Linda University Electronic Theses, Dissertations & Projects

The development and description of a quantitative method of evaluating streptococcal antibodies by inhibition of fluorescent staining is reported. Suspensions of Streptococcus pyogenes group A, treated with methyl alcohol to reduce auto-fluorescence, were used as antigen. The degree of inhibition of uptake of fluorescent labeled antibody produced by the presence of antibodies in the immune test sera was measured after elution of the labeled antibody. Comparisons were made with controls using normal sera containing no inhibiting antibodies. A method of reporting the results in terms of titer is described.

Streptococcal antibodies were demonstrated quantitatively by this inhibition test in sera …

Lambing Trials At Abydos Research Station, H Suijdendorp

Journal of the Department of Agriculture, Western Australia, Series 4

ABYDOS Research Station is halfway between Port Hedland and Wittenoom Gorge. I t is in country of granitic origin that is largely covered with spinifex (mainly Triodia pungens) and associated species.

The results of a recent lambing trial at Abydos are discussed below:

Field Day : Poultry Research : A Progress Report : Poultry Research Station, Wembley, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

AT the fourth field day held at the Department of Agriculture's Poultry Research Station at Wembley recently, about 150 visitors, most of them commercial poultry farmers, inspected the station and saw the progress of the comprehensive research programme being carried out by officers of the Department's Poultry Branch.