Animal Experimentation and Research Commons^™

Differential Strain-Dependent Ovarian And Metabolic Responses In A Mouse Model Of Pcos, April K. Binder, Danielle L. Peecher, Amanda J. Qvigstad, Silvia D. Gutierrez, Jennifer Magaña, David B. Banks, Kenneth S. Korach

Biology Faculty Scholarship

Several mouse models have been developed to study polycystic ovarian syndrome (PCOS), a leading cause of infertility in women. Treatment of mice with dihydrotestosterone (DHT) for 90-days causes ovarian and metabolic phenotypes similar to women with PCOS. We used this 90-day DHT treatment paradigm to investigate the variable incidence and heterogeneity in two inbred mouse strains, NOD/ShiLtJ and 129S1/SvlmJ. NOD mice naturally develop type 1 diabetes, and recent meta-analysis found increased androgen excess and PCOS in women with type 1 diabetes. 129S1 mice are commonly used in genetic manipulations. Both NOD and 129S1 DHT treated mice had early vaginal opening, …

Comparison Of Percutaneous Vs Oral Infection Of Hamsters With The Hookworm Ancylostoma Ceylanicum: Parasite Development, Pathology And Primary Immune Response, Richard D. Bungiro, Lisa M. Harrison, Blaise Dondji, Michael Cappello

Biology Faculty Scholarship

Background

Hundreds of millions of people in poor countries continue to suffer from disease caused by bloodfeeding hookworms. While mice and rats are not reliably permissive hosts for any human hookworm species, adult Golden Syrian hamsters are fully permissive for the human and animal pathogen Ancylostoma ceylanicum. Similar to humans, hamsters may be infected with A. ceylanicum third-stage larvae orally or percutaneously. Oral infection typically leads to consistent worm yields in hamsters but may not accurately reflect the clinical and immunological manifestations of human infection resulting from skin penetration.

Methodology/Principal findings

In this study we compared host responses …

Effect Of Group Size On The Activity Budget Of Two Captive Chimpanzees (Pan Troglodytes), Kaeley Sullins

All Master's Theses

Captive facilities housing chimpanzees are required to provide adequate care and provisions such as dietary, social, and environmental enrichment to promote the psychological well-being of the apes in their care. Chimpanzees are social creatures and changes in groups as well as relocation to a facility with new social partners, can impact each individual chimpanzee’s welfare. By tracking each chimpanzee’s activity budgets, managers can assess welfare and make improvements or adjustments if necessary. I looked at the activity budgets of two captive chimpanzees after the death of a group member and the two chimpanzees’ subsequent relocation to a novel, more socially …

Cold Tolerance, Temperature Mediated Discontinuous Gas Exchange, And Emergence Of The Blue Orchard Mason Bee (Osmia Lignaria), Logan Kral

All Master's Theses

The relationship between low temperatures, emergence and supercooling point of Osmia lignaria were the subject of this study. One hundred sixty-eight bees were subjected to 5 pre-wintering temperature treatments (two constant temperature controls - 22^oC, 14^oC, one of which with and one without a 12h photoperiod, and three 12h:12h thermoperiod treatments – 14:10^oC, 14:5^oC, and 14:0^oC) and were then evaluated in terms of emergence time and post-emergence vigor. An additional 70 bees were tested for metabolic rate and discontinuous gas exchange in response to test temperature conditions. An additional sample …

Genetic Analysis Of Putative Familial Relationships In A Captive Chimpanzee (Pan Troglodytes) Population, Renato Robledo, Joseph G. Lorenz, Jeanne Beck, James Else, Patrick Bender

Anthropology and Museum Studies Faculty Scholarship

Twelve autosomal dinucleotide repeat loci were analyzed in chimpanzees genomes by DNA amplification using primers designed for analysis of human loci. The markers span the entire length of human chromosomes 21 and 22. Nine markers were polymorphic in chimpanzee as well, with a somewhat comparable level of polymorphism and allele size range. Even in the presence of very limited information and in spite of missing samples, it was possible to reconstruct a complex pedigree and to provide molecular data that corroborate family relationships that were deduced from cage history and behavioral data. The conclusions were further supported by mitochondrial DNA …

State-Dependent Learning As A Function Of The Temporal Relationship Between Noncontingent Footshock And Electroconvulsive Shock, T. Scott Shutt

All Master's Theses

Rats were given a noncontingent footshock followed at various intervals by electroconvulsive shock. Twentyfour hours later they were trained on a non-shock passive avoidance task and tested for retention 72 hours later. When the interval between NCFS and ECS was short the animals showed an amnesia which reduced as the interval was lengthened. An interval of .5 seconds produced the most pronounced amnesia and intervals greater than 10 seconds produced virtually no amnesia. The results were consistent with a state dependent retrieval failure hypothesis.

The Cathartic Effects Of Aggressive Responses Directed Toward An Inanimate Object, Timothy William Creston

All Master's Theses

This study was intended to test the catharsis of aggression hypothesis which in part holds that aggressive responses directed towards an inanimate object will have a tension reducing effect and result in a lowering of residual hostility.

The Effects Of Cs Habituation And Duration Of The Directional Modification Of The Cardiac Response In The Guinea Pig (Cavia Procellus), Ray Charles Larson

All Master's Theses

The present experiment examined the effects of CS habituation and two different levels of CS duration (60 versus 6 sec) on the directional modification of the conditioned cardiac response in the guinea pig. The results indicated that the only reaction to the CS, regardless of prior habituation or CS duration, was a decelerative conditioned HR response. However, habituation to a CS prior to classical aversive conditioning was found to attenuate the magnitude of the bradycardia CR that developed. The bradycardia CR was discussed in terms of being a cardiac component of the orienting response.

Sub-Aversive Response Contingent Foot Shock As A Positive Reinforcer, Robert Lea Fulwiler

All Master's Theses

Forty-eight rats were divided into 8 groups; four were maintained under normal conditions and the other four under sensory deprivation and tested at o, 3, 6, and 9 days after condition institution. The response was placing the head through a hole in the operant chamber and the stimulus (0, 1.2, 4, or 12 Vac) was contingent upon the response. Analysis ot variance disclosed significant differences (p<.01) between the deprived and the non-deprived groups at days 6 and 9; and a significant interaction between deprivation condition and time of test. No differences were shown between the stimulus intensities indicating that the stimulus did not have a reinforcing effect.

Chelonian Brain-Membranes, Brain-Bladder, Metapore And Metaplexus, John P. Munson

Biology Faculty Scholarship

The chelonian brain is not too small to be studied macroscopically. It is easily removed from the skull. Minute surface details and the cell structure can be easily observed.