Botany Commons^™

Productivity Of Field Pea (Pisumsativum L.) And Spring Oat (Avena Sativa L.) Grown As Sole And Intercrops Under Different Nitrogen Levels, Chengchou Han, Christopher Borman, Dori Osantowski, Jeremy Wagnitz, Katja Koehler-Cole, Kevin Korus, Evan Sonderegger, Rodrigo Werle, Tara Wood, John L. Lindquist

Department of Agronomy and Horticulture: Faculty Publications

Intercropping cereal grains and legumes has potential as an alternative cool-season forage crop in low-input farming systems. The objectives of this study were to quantify the effects of density, species proportion, and nitrogen (N) supply on the biomass accumulation of field pea and spring oat grown as sole or intercrops. Greenhouse experiments were conducted using an additive series experimental design. Treatments included three fertilizer N rates and 20 density/proportion combinations in a randomized complete block with four replications. Sole plant densities included 2, 3, 4, 5 plants of each species pot-1 (16 cm in diameter, 16 cm in height). The …

Inheritance Of Grain Polyphenol Oxidase (Ppo) Activity In Multiple Wheat (Triticum Aestivum L.) Genetic Backgrounds, Somrudee Nilthong, Robert A. Graybosch, P. Stephen Baenziger

Department of Agronomy and Horticulture: Faculty Publications

Grain polyphenol oxidase (PPO) activity can cause discoloration of wheat (Triticum aestivum L.) food products. Five crosses (PI 117635/Antelope; Fielder/ NW03681; Fielder/Antelope; NW07OR1070/Antelope; NW07OR1066/OR2050272H) were selected to study the genetic inheritance of PPO activity. STS markers, PPO18, PPO29 and STS01, were used to identify lines with putative alleles at the Ppo-A1 and Ppo-D1 loci conditioning low or high PPO activity. ANOVA showed significant genotypic effects on PPO activity (P\0.0001) in all populations. The generations and generation 9 genotype effects were not significant in any population. A putative third (null) genotype at Ppo-A1 (no PCR fragments for PPO18) was discovered …

Identification And Characterization Of Four Missense Mutations In Brown Midrib 12 (Bmr12), The Caffeic O-Methyltranferase (Comt) Of Sorghum, Scott E. Sattler, Nathan A. Palmer, Ana Saballos, Ann M. Greene, Zhanguo Xin, Gautam Sarath, Wilfred Vermerris, Jeffrey F. Pedersen

Department of Agronomy and Horticulture: Faculty Publications

Modifying lignin content and composition are targets to improve bioenergy crops for cellulosic conversion to biofuels. In sorghum and other C4 grasses, the brown midrib mutants have been shown to reduce lignin content and alter its composition. Bmr12 encodes the sorghum caffeic O-methyltransferase, which catalyzes the penultimate step in monolignol biosynthesis. From an EMS-mutagenized TILLING population, four bmr12 mutants were isolated. DNA sequencing identified the four missense mutations in the Bmr12 coding region, which changed evolutionarily conserved amino acids Ala71Val, Pro150Leu, Gly225Asp, and Gly325Ser. The previously characterized bmr12 mutants all contain premature stop codons. These newly identified mutants, along …

Cultivating A Movement: Excerpts From An Oral History Of Organic Farming And Sustainable Agriculture On California’S Central Coast [Book Review], Charles A. Francis

Department of Agronomy and Horticulture: Faculty Publications

Edited by I. Reti and S. Rabkin, 2011. University of California, Santa Cruz, Library, Santa Cruz, California, United States. 299 p., US$19.95, ISBN 9-780972-33431, paper.

Often the most compelling evidence for success of organic farming comes from the personal stories of farmers. Coupled with reports on the application of science in organics, the practical knowledge of people in the field provides a rich foundation for the ongoing growth of this intriguing sector of the food system. This collection of interviews by the staff of the Regional History Project is one unique activity of the University of California, Santa Cruz library, …

Estimating Construction Costs For A Low-Cost Quonset-Style Greenhouse, David P. Lambe, Stacy A. Adams, Ellen T. Paparozzi

Department of Agronomy and Horticulture: Faculty Publications

Double polyethylene Quonset-style greenhouses offergrowers and farmers a more cost-effective structure than glass- or acrylic-covered structures for growing plants off-season. These greenhouses are especially applicablefor producing off-season high value crops such as fruits and vegetables for local markets. To support this production alternative, this publication contains a list of greenhouse components, the start-up costs associated with these items and an estimate of the number of person hours required to assemble and outfit a 24-foot-by-72-foot double polyethylene, air-inflated greenhouse structure. As there are many options available for building double polyethylene greenhouses, the main purpose of this publication is to identify the …

The ‘Pi 438489b’ By ‘Hamilton’ Snp-Based Genetic Linkage Map Of Soybean [Glycine Max (L.) Merr.] Identified Quantitative Trait Loci That Underlie Seedling Sds Resistance, Kassem My Abdelmajid, Laura Ramos, Leonor Leandro, Gladys Mbofung, D. L. Hyten, Stella K. Kantartzi, Robert L. Grier Iv, Victor N. Njiti, Khalid Meksem

Department of Agronomy and Horticulture: Faculty Publications

Soybeans [Glycine max (L.) Merr.] are susceptible to many diseases including fungal diseases such as soybean sudden death syndrome (SDS). Several studies reported SDS resistance quantitative trait loci (QTL) on the soybean genome using different recombinant inbred line (RIL) populations and low density genetic linkage maps. High density exclusively single nucleotide polymorphisms-based (SNP-based) maps were not yet reported in soybean. The objectives of this study were (1) to construct a high density SNP-based genetic linkage map of soybean using the ‘PI438489B’ by ‘Hamilton’ (PIxH, n=50) recombinant inbred line population, and (2) to map QTL for SDS resistance using this …

Structural Variants In The Soybean Genome Localize To Clusters Of Biotic Stress-Response Genes, Leah K. Mchale, William J. Haun, Wayne W. Xu, Pudota B. Bhaskar, Justin E. Anderson, D. L. Hyten, Daniel J. Gerhardt, Jeffrey A. Jeddeloh, Robert M. Stupar

Department of Agronomy and Horticulture: Faculty Publications

Genome-wide structural and gene content variations are hypothesized to drive important phenotypic variation within a species. Structural and gene content variations were assessed among four soybean (Glycine max) genotypes using array hybridization and targeted resequencing. Many chromosomes exhibited relatively low rates of structural variation (SV) among genotypes. However, several regions exhibited both copy number and presence-absence variation, the most prominent found on chromosomes 3, 6, 7, 16, and 18. Interestingly, the regions most enriched for SV were specifically localized to gene-rich regions that harbor clustered multigene families. The most abundant classes of gene families associated with these regions …

Identification Of Positive Yield Qtl Alleles From Exotic Soybean Germplasm In Two Backcross Populations, K.-S. Kim, B. W. Diers, D. L. Hyten, M. A. Rouf Mian, J. G. Shannon, R. L. Nelson

Department of Agronomy and Horticulture: Faculty Publications

Increasing seed yield is an important breeding goal of soybean [Glycine max (L.) Merr.] improvement efforts. Due to the small number of ancestors and subsequent breeding and selection, the genetic base of current soybean cultivars in North America is narrow. The objective of this study was to map quantitative trait loci (QTL) in two backcross populations developed using soybean plant introductions as donor parents. The first population included 116 BC₂F₃-derived lines developed using ‘‘Elgin’’ as the recurrent parent and PI 436684 as the donor parent (E population). The second population included 93 BC₃F …

Molecular Mapping Of Soybean Rust Resistance In Soybean Accession Pi 561356 And Snp Haplotype Analysis Of The Rpp1 Region In Diverse Germplasm, Ki-Seung Kim, Jair R. Unfried, D. L. Hyten, Reid D. Frederick, Glen L. Hartman, Randall L. Nelson, Qijian Song, Brian W. Diers

Department of Agronomy and Horticulture: Faculty Publications

Soybean rust (SBR), caused by Phakopsora pachyrhizi Sydow, is one of the most economically important and destructive diseases of soybean [Glycine max (L.) Merr.] and the discovery of novel SBR resistance genes is needed because of virulence diversity in the pathogen. The objectives of this research were to map SBR resistance in plant introduction (PI) 561356 and to identify single nucleotide polymorphism (SNP) haplotypes within the region on soybean chromosome 18 where the SBR resistance gene Rpp1 maps. One-hundred F_2:3 lines derived from a cross between PI 561356 and the susceptible experimental line LD02-4485 were genotyped with genetic …

Advances In Genome Sequencing And Genotyping Technology For Soybean Diversity Analysis, David L. Hyten

Department of Agronomy and Horticulture: Faculty Publications

The completion of a soybean draft whole genome sequence along with advances in sequencing and genotyping technologies are creating a revolution in soybean genomics. The draft whole genome sequence of soybean is allowing researchers to fully take advantage of this new technology and is rapidly changing how soybean germ plasm is mined. Genetic markers such as SNPs can be quickly identified by using next generation sequencing and assayed on a large number of materials using advanced technologies that can genotype tens of thousands of markers on thousands of individuals very rapidly. This ability to qUickly identify and genotype genetic diversity …

High-Resolution Mapping Of Open Chromatin In The Rice Genome, Wenli Zhang, Yufeng Wu, James C. Schnable, Zixian Zeng, Michael Freeling, Gregory E. Crawford, Jiming Jiang

Department of Agronomy and Horticulture: Faculty Publications

Gene expression is controlled by the complex interaction of transcription factors binding to promoters and other regulatory DNA elements. One common characteristic of the genomic regions associated with regulatory proteins is a pronounced sensitivity to DNase I digestion. We generated genome-wide high-resolution maps of DNase I hypersensitive (DH) sites from both seedling and callus tissues of rice (Oryza sativa). Approximately 25% of the DH sites from both tissues were found in putative promoters, indicating that the vast majority of the gene regulatory elements in rice are not located in promoter regions. We found 58% more DH sites in …

Review Of Teaching Children Science: Hands-On Nature Study In North America, 1890-1930 By Sally Kohlstedt, Meena M. Balgopal

The Prairie Naturalist

Many scientists and educators agree that the goal of science education is to prepare students "to know, use and interpret scientific explanations of the natural world," as cited in the National Research Council publication, Taking Science to School: Learning and Teaching Science in Grades K-8 (DuschI et al. 2007). Yet, many science instructors of K-12 and post-secondary students often rely on teacher-telling modes of pedagogy and neglect to engage their students in natural inquiry and scientific study that model the research methods used by scientists. As a result, many young people are not aware of how scientists make discoveries about …

Genetic Structure Of Grass Carp Populations In The Missouri And Mississippi River Basins, Usa, Bobbi M. Adams, Katie N. Bertrand, Michael L. Brown, Donald Auger

The Prairie Naturalist

We provided an early characterization of the genetic structure of the grass carp (Ctenopharyngodon idella) population as it expands its distribution in both the Missouri and Mississippi River basins. Further, we provided initial comparisons of allelic richness at 17 polymorphic microsatellite markers between 56 grass carp from the USA, and six from the Yangtze River in China. The number of alleles per locus ranged from two to eight and size ranges of alleles for fish collected from the invaded and native ranges were similar (P ≤ 0.001; 107–226 bp) to those previously reported in the literature. Distance-based clustering …

Massasauga Repatriation On A Restored Wet Prairie, Francis E. Durbian, Brian N. Lomas, Jeff Briggler, Paul Mckenzie, Tom Nagel

The Prairie Naturalist

The massasauga (Sistrurus catenatus) is a small rattlesnake that occurs from Texas to New York (Schmidt and Davis 1941, Conant and Collins 1991, Szymanski 1998). In Missouri, the Eastern massasauga rattlesnake (EMR; S. c. catenatus), a subspecies of the massasauga, occurs north and east of the Missouri River, is a former candidate for listing under the United States Endangered Species Act (Code of Federal Regulations 64 FR 57534; Szymanski 1998), and is listed as a state endangered species (Missouri Natural Heritage Program 2011). Missouri currently harbors five extant EMR populations (Johnson 2000, Durbian et al., unpublished report, …

Book Review Of Encyclopedia Of Biological Invasions, Edited By Daniel Simberloff And Marcel Rejmanek, Qinfeng Guo

The Prairie Naturalist

Book review of Encyclopedia of Biological Invasions, edited by Daniel Simberloff and Marcel Rejmanek.

Species introductions and consequent biotic invasions and homogenization are major components of global change that are drawing increasing concern and various levels of actions and reactions around the world. Invasion ecology has advanced rapidly during the last few decades, and the discipline is now increasingly integrated with the social and economic sciences. A better understanding of the invasion process and its effects is thus clearly needed. For basic research, invasion biology offers fascinating and sometimes unique opportunities for testing certain ecological or evolutionary theories and …

Notes On The Distribution Of Eastern Woodrats And Hispid Cotton Rats In South-Central Nebraska, Heather D. Wills, Keith Geluso, Eric J. Smits, Joseph T. Springer, Wesley E. Newton

The Prairie Naturalist

The eastern woodrat (Neotoma floridana) and hispid cotton rat (Sigmodon hispidus) reach distributional limits in southern Nebraska (Jones 1964, Farney 1975). In the last half-century, both species have expanded their distributional ranges in the region (Kugler and Geluso 2009, Wright et al. 2010). Herein, we report new localities of occurrence for both species that extend known distributional boundaries in south-central Nebraska beyond those reported by Kugler and Geluso (2009) and Wright et al. (2010).

Associated with other studies, we captured small mammals in Sherman live-traps in south-central Nebraska. Voucher specimens were deposited in collections at the …

Comparison Of Fish Communities In Recently Constructed Side-Channel Chutes With The Main Stem Missouri River, Kasey Whiteman, Vincent H. Travnichek, Darrick L. Garner, Brandon Eder, Kirk Steffensen

The Prairie Naturalist

Two United States Army Corp of Engineers- (USACE) funded projects were conducted from 2006 to 2008 along the Missouri River to monitor fish communities in recently constructed side-channel chutes and to monitor pallid sturgeon (Scaphirhynchus albus) and the associated fish assemblage in the main stem Missouri River. Data from both monitoring projects were compared to evaluate fish assemblages among four mitigated habitats (e.g., constructed side-channel chutes) and the main-stem Missouri River. Chutes had a greater overall number of species (n = 59) and higher species richness (Margalef’s index = 5.81), but richness was not different (F1, 4 = …

Patterns Of Age-0 Gizzard Shad Abundance And Food Habits In A Nebraska Irrigation Reservoir, Christopher L. Sullivan, Casey W. Schoenebeck, Keith D. Koupal, W. Wyatt Hoback, Brian C. Peterson

The Prairie Naturalist

Gizzard shad (Dorosoma cepedianum) are prolific spawners that can influence reservoir communities. Larval gizzard shad may compete with larval recreational fish for zooplankton resources. Therefore, it is necessary to determine larval gizzard shad dynamics and food habits to better understand their potential for competition with larval recreational fish. Our study examined age-0 gizzard shad abundance in Harlan County Reservoir during late spring/summer from 2002–2010 and food habits and prey electivity of age-0 gizzard shad during late spring/summer 2008 and 2009. The annual peak age-0 gizzard shad density 3 from 2002–2010 ranged from 50 to 380/100 m , which …

Northern Harrier Hatches Mallard Nest, Jeffery W. Stackhouse, Benjamin A. Geaumont

The Prairie Naturalist

The northern harrier (Circus cyaneus) is a widespread raptor commonly found nesting throughout the Northern Great Plains. Northern harriers forage primarily on small mammals and passerines with the males providing the majority of prey to nestlings until 14–28 days of age (Redpath et al. 2006, Vukovich and Ritchison 2006). Although the genus Circus is known to predate eggs from nest of many nesting birds (Hiraldo et al. 1975, Donaszar et al. 1996, Opermanis et al. 2000), few have been recorded commandeering nests of different species (Laine 1928, Fleskes 1992). In June 2010, we observed a northern harrier that …

Late Seasonal Captures Of The Plains Pocket Mouse (Perognathus Flavescens) In Iowa, Hans W. Otto, Jeremy A. White

The Prairie Naturalist

The plains pocket mouse, Perognathus flavescens, is a nocturnal granivore that inhabits friable soils in sparsely vegetated areas (Monk and Jones 1996). In the United States, the distribution of the plains pocket mouse extends from Arizona, New Mexico, and Texas through the Great Plains to North Dakota and Minnesota (Hall 1981). This species reaches its easternmost limits in Iowa, where it has been documented from a limited number of grassland sites in eastern, central, and western parts of the state (Bowles 1975, Wilson et al. 1996). Due to loss of grassland habitat, the plains pocket mouse is currently listed …

Review Of Sandhill And Whooping Cranes: Ancient Voices Over America's Wetlands By Paul Johnsgard, Jane E. Austin

The Prairie Naturalist

Paul Johnsgard has long been captivated by wild cranes and their unique vocalizations, courtship dances, and wide-ranging migrations. As a scientist and an admirer, Johnsgard has watched their migrations and behaviors for decades as hundreds of thousands of cranes staged each spring by the central Platte River, not far from his home in Lincoln, Nebraska. As an artist, he has skillfully captured their courtship dances and other behaviors in his exceptional line drawings. And, as an author, he has written extensively on their ecology in three earlier books: Cranes of the World (1983), Those of the Gray Wind: The Sandhill …

Depth And Littoral Habitat Association Of Age-0 Yellow Perch In Two South Dakota Glacial Lakes, Daniel J. Dembkowski, Melissa R. Wuellner, David W. Willis

The Prairie Naturalist

Yellow perch (Perca flavescens) are a recreationally important species and represent a key ecological component of glacial lake littoral fish assemblages (Stone 1996, Blackwell et al. 1999). Research has shown a generalized pattern of juvenile (age-0) yellow perch spatial distribution wherein larvae hatch in near-shore areas, migrate to limnetic areas where they remain for approximately 40 d, and then return to demersal behaviors and within near-shore littoral habitats (Noble 1975, Whiteside et al. 1985). However, anomalous distribution and habitat use by age-0 yellow perch has been observed in South Dakota glacial lakes (Fisher and Willis 1997) and the …

Book Review: Effects Of Climate Change On Birds, Edited By Anders Pape Meller, Wolfgang Fiedler, And Peter Berthold, Brian J. Olsen

The Prairie Naturalist

Book review of Effects of Climate Change on Birds, edited by Anders Pape Meller, Wolfgang Fiedler, and Peter Berthold.

Climate scientists from across the globe predict vast changes during the next century in the planet's temperatures, precipitation, storm intensities, fire regimes, hydrologic cycles, and atmospheric, water, and soil chemistries. The changes will be global, but their effects will be felt locally everywhere. To find a scientifically and socially engaging bellwether of these events, we need to look only through the lens of avian biology. Birds are found from pole to pole; their movements connect continents. They are loud, colorful …

Modeling Parental Provisioning By Red-Winged Blackbirds In North Dakota, George M. Linz, Richard S. Sawin, Mark W. Lutman, William J. Bleier

The Prairie Naturalist

Male red-winged blackbirds (Agelaius phoeniceus) exhibit a difference in nest provisioning rates along an east-west gradient in North America. North Dakota is located in the center of North America and harbors a large population of breeding red-winged blackbirds (RWBL). This location provided an opportunity to compare provisioning rates in the central U.S. with those reported for the eastern and western populations. We placed video cameras at RWBL nests to record male and female feeding trips. Thirty-four nests were located on territories with original males and 30 were on territories where a replacement (floater) male had taken over a …

Manuscript Content: Where Does It Belong?, Christopher N. Jacques

The Prairie Naturalist

One of the most common issues we address during content editing of papers for The Prairie Naturalist (journal) is the appropriate placement of content in the text body. Based on my experience with the editorial process, content placement also is one of the issues that authors are most resistant to suggestions or do not fully understand why we are so persistent about it (Thompson 2010). One of the primary objectives of scientific writing is to concisely and accurately disseminate information. Scientific papers are structured to help both the author and reader accomplish this objective. The material that belongs in each …

Heritable Epigenetic Variation Among Maize Inbreds, Steve R. Eichten, Ruth A. Swanson, James C. Schnable, Amanda J. Waters, Peter J. Hermanson, Sanzhen Liu, Cheng-Ting Yeh, Yi Jia, Karla Gendler, Michael Freeling, Patrick S. Schnable, Matthew W. Vaughn, Nathan M. Springer

Department of Agronomy and Horticulture: Faculty Publications

Epigenetic variation describes heritable differences that are not attributable to changes in DNA sequence. There is the potential for pure epigenetic variation that occurs in the absence of any genetic change or for more complex situations that involve both genetic and epigenetic differences. Methylation of cytosine residues provides one mechanism for the inheritance of epigenetic information. A genome-wide profiling of DNA methylation in two different genotypes of Zea mays (ssp. mays), an organism with a complex genome of interspersed genes and repetitive elements, allowed the identification and characterization of examples of natural epigenetic variation. The distribution of DNA methylation …

Effects Of Herbicides And Grazing On Floristic Quality Of Native Tallgrass Pastures In Eastern South Dakota And Southwestern Minnesota, Alexander J. Smart, Matthew J. Nelson, Peter J. Bauman, Gary E. Larson

Great Plains Research: A Journal of Natural and Social Sciences (through 2013)

Historic herbicide use and grazing have influenced natural diversity and quality of native pasturelands in the Great Plains. Floristic quality assessments are useful to assist agencies in prioritizing conservation practices to enhance native grasslands. The objective of this study was to determine the effects of past land-use practices on the floristic quality of remnant native pastures in eastern South Dakota and southwestern Minnesota. Floristic quality assessments were conducted on 30 native pastures and categorized by past management practices (herbicide application and grazing intensity). Mean coefficient of conservatism (C) and floristic quality index (FQI) were calculated for each site~Results showed that …

Use Of Natural Variation Reveals Core Genes In The Transcriptome Of Iron-Deficient Arabidopsis Thaliana Roots, Ricardo J. Stein, Brian M. Waters

Department of Agronomy and Horticulture: Faculty Publications

Iron (Fe) is an essential mineral micronutrient for plants and animals. Plants respond to Fe deficiency by increasing root uptake capacity. Identification of gene networks for Fe uptake and homeostasis could result in improved crop growth and nutritional value. Previous studies have used microarrays to identify a large number of genes regulated by Fe deficiency in roots of three Arabidopsis ecotypes. However, a large proportion of these genes may be involved in secondary or genotype-influenced responses rather than in a universal role in Fe uptake or homeostasis. Here we show that a small percentage of the Fe deficiency transcriptome of …

Miscanthus 3 Giganteus Productivity: The Effects Of Management In Different Environments, Matt Maughan, German Bollero, D.K. Lee, Robert Darmody, Stacy Bonos, Laura Cortese, James Murphy, Roch E. Gaussoin, Matthew Sousek, David Williams, Linda Williams, Fernando Miguez, Thomas Voigt

Department of Agronomy and Horticulture: Faculty Publications

Miscanthus 9 giganteus is a C4 perennial grass that shows great potential as a high-yielding biomass crop. Scant research has been published that reports M. 9 giganteus growth and biomass yields in different environments in the United States. This study investigated the establishment success, plant growth, and dry biomass yield of M. 9 giganteus during its first three seasons at four locations (Urbana, IL; Lexington, KY; Mead, NE; Adelphia, NJ) in the United States. Three nitrogen rates (0, 60, and 120 kg ha -1) were applied at each location each year. Good survival of M. 9 giganteus during its first …

Effects Of Species, Water, And Nitrogen On Competition Among Three Prairie Grasses, Jerry L. Weatherford, Randall W. Myster

The Prairie Naturalist

We conducted an experiment to investigate effects of species, water (W), and nitrogen (N) on competition among little bluestem (Schizachyrium scoparium), sideoats grama (Bouteloua curtipendula), and indiangrass (Sorghastrum nutans). All biomass parameters and the root:shoot ratio of little bluestem were reduced by the presence off 1 of 2 other species, and its shoot biomass and total biomass were both increased by addition of N. Root and shoot biomass of sideoats grama were reduced by the presence of indiangrass and its total biomass was reduced by the presence of itself, whereas its shoot biomass was increased by addition of W at …