Agricultural Science Commons^™

Purification And Characterization Of Intrauterine Folate-Binding Proteins From Swine, J.L. Vallet, R.K. Christenson, H.G. Klemcke

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Folate-binding proteins (FBP) from Day 60 pseudopregnant uterine flushings and Day 60 allantoic fluid were purified by affinity chromatography on folate-Sepharose followed by G–100 Sephadex chromatography. FBP from uterine flushings had a molecular weight of 20 000; the N-terminal sequence was FNWDHXGKMEPAXKRHFXXXTXLYX, which is 72% identical to bovine milk FBP beginning at amino acid 64. Allantoic fluid FBP had a molecular weight of 30 000; and the N-terminal sequence ARAKTDMLNVXMDAKHHKPKPSXED, which is 68% identical to bovine milk FBP starting at amino acid 4. Scatchard analysis of purified allantoic fluid FBP using [³H]folic acid as ligand indicated a dissociation …

Focus On The Future Agriculture Western Australia's Strategic Directions 1998 - 2008, Agriculture Western Australia

Reports

Strong growth in global demand combined with productivity gains, product innovation and sustainability improvements throughout the 1990s enable Western Australia agriculture to look to the 21st century with confidence.

This optimistic outlook is largely the result of innovation across the agricultural industries based on research, development and extension - activities in which Agriculture Western Australia continues to be the State's leading provider.

Focus on the Future is ambitious, and sends a unequivocal message about our strategic directions and the rationale guiding the agency in allocating resources to those areas which offer the biggest benefits to the Western Australian agriculture, food …

Western Australian Agriculture - Key Achievements 1997-1998, Department Of Primary Industry And Fisheries, Western Australia

Farm Systems and Economics published reports

During the past year the agriculture sector continued to be one of the most innovative and significant contributors to the Western Australian economy. In 1996/97 the value of agricultural exports reached $4.6 billion, having doubled in five years.

Not only does agriculture contribute directly to the State's financial position but farm expenditure is estimated to have a three-fold multiplier effect, thereby having a wide impact on the social well-being of Western Australians.

Increasingly, international trade is being linked to environmental performance. Accordingly, the Government has cemented sustainable farming as a cornerstone of the industry's and Agriculture WA's activities, thereby ensuring …

Plant Hemoglobins, Raul Arrendondo-Peter, Mark S. Hargrove, Jose F. Moran, Gautam Sarath, Robert V. Klucas

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Hbs are ubiquitous proteins in most organisms, including bacteria, protozoans, fungi, plants, and animals (Vinogradov et al., 1993; Bolognesi et al., 1997). The known and predicted roles of Hbs in each organism encompass functions that include the reversible binding of gaseous ligands and the ability to bind other cellular molecules.

Selective And Organotypic Culture Of Intrahepatic Bile Duct Cells From Adult Pig Liver, Neil C. Talbot, Thomas J. Caperna

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Secondary culture of nontransformed bile duct epithelium has been difficult to achieve. STO feeder cell-dependent secondary cultures of adult pig bile duct cells were established from primary cultures of adult pig liver cells. Adult pig hepatocytes exhibited limited or no replication and were lost from the secondary culture at Passage 3 or 4. In contrast, adult pig bile duct cells replicated and were carried for 4-8 passages in secondary culture. A simple method to produce nearly pure pig intrahepatic bile duct cultures was first to freeze a relatively crude liver cell preparation. Upon subsequent thawing, all hepatocytes and most macrophages …

Growth Traits Among Sorghum Genotypes In Response To Aluminum, J. H. Bernal, R. B. Clark

Department of Agronomy and Horticulture: Faculty Publications

Aluminum (Al) has many detrimental effects on plant growth, and shoots and roots are normally affected differently. A study was conducted to determine differences among sorghum [Sorghum bicolor (L.) Moench] genotypes with broad genetic backgrounds for growth traits of plants grown at 0,200,400,600, and 800 uM Al in nutrient solutions (pH 4.0). Genotypes were categorized into "Al-sensitive", "intermediate Al-tolerant", "Al-tolerant", and SC 283 (an Al-tolerant standard). As Al increased, shoot and root dry matter (DM), net main axis root length (NMARL), and total root length (TRL) became lower than controls (0 Al). Aluminum toxicity and/or nutrient deficiency symptoms become …

Atrazine, Deethylatrazine, And Deisopropylatrazine Persistence Measured In Groundwater In Situ Under Low-Oxygen Conditions, Sharon Papiernik, Roy Spalding

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The degradation of atrazine [2-chloro-4-(ethylamino)-6-isopropylamino-1,3,5-triazine], deethylatrazine [DEA; 2-amino-4-chloro-6-(isopropylamino)-1,3,5-triazine], and deisopropylatrazine [DIA; 2-amino-4-chloro-6-(ethylamino)-1,3,5-triazine] was assessed under limited oxygen conditions using in situ microcosms. Denitrification was induced in a shallow sand and gravel aquifer to measure the potential for degradation of atrazine, DEA, and DIA under low-O2 conditions. The dissolved oxygen content decreased from 7-8 mg/L to ≤1 mg/L within 4 days and remained ≤ mg/L for the remainder of the 45-day experiment. Atrazine, DEA, and DIA concentrations (normalized to the bromide concentration at each sampling time to account for dilution) did not show a significant decrease with time, indicating that these …

Technique For Rlem Screening In Glasshouse, Krzysztof S. Gajda

Grain and other field crops published reports

The redlegged earth mite (RLEM) Halotydeus destructor (Tucker) (Acarina: Penthaleidae) is an introduced, major pest of crops and pastures across south Australia. RLEM adversely effect pasture by destroying legume seedlings in germinating pastures and reducing pasture production and seed yield. One of the ways to reduce the effect of RLEM is by breeding legume cultivars with RLEM resistance. This involves screening of many hundreds of potentially resistant plants and lines in a short time. In WA, at Agriculture Western Australia, South Perth, a screening program for resistance to RLEM in subterranean clover (Trifolium subterranean) and annual medics (Medicago ssp.) has …

Evidence Of Antibiosis Resistance To The Spotted Stemborer, Chilo Partellus (Swinhoe) In A Maize (Zea Mays L.) Population, C. J.M. Mutinda, K. N. Saxena, S. O. Ajala, P. O. Aviecho

All Peer-Reviewed Publications

S2 line and S2 test-cross recurrent selection procedures were initiated to improve the resistance of a maize (Zen mays L.) population to the spotted stemborer, Chllo partclltis (Swinhoe) (Lepidoptera: Pyralidae). Thirty-five selections developed from the population were evaluated for resistance to the stemborer in western Kenya. Resistance was rated according to leaf feeding (LF), stem tunnelling (ST) and deadheart formation (DH) following artificial infestation with first-instar C. pnrtcUus. Larval establishment and growth were also assessed. The reduction in foliar damage ratings, percentage stem length tunnelled, and percentage of plants showing deadheart after one cycle of recurrent selection were 4.38 %, …

Morphological Study Of Cultivated Cowpea Vigna Unguiculata (L.) Walp. Importance Of Ovule Number And Definition Of Cv Gr Melanophthalmus, Rémy Stéphane Pasquet

All Peer-Reviewed Publications

More than 400 cultivated cowpea accessions were studied using 29 morphophysiological characters. The analysis of data contrasts evolved cultivars and more primitive cultivars (and wild forms) according to seed size on the one hand and early and late flowering (under inductive conditions) on the other hand. The latter character is markedly correlated with ovule number. Photosensitive and early-flowering photoindependent cultivars have 11-17 ovules per pod and late flowering photoindependent cultivars have 16-25 ovules per pod. The analysis leads to assigning the accessions studied to the following different cultigroups: cv gr Unguiculata, cv gr Sesquipedalis, cv gr Biflora and cv gr …

Inactivation Of Soybean Peroxidase During Sodium Azide Oxidation: A Comparative Study, Mohamed Yehia Zakaria Aboul Eish

Dissertations and Theses @ UNI

Azide ion is a mechanism-based inactivator of a number of peroxidases. However, there are often variations in the manner in which inactivation occurs. Soybean Peroxidase (SBP) is found in the plant's seed coat. Unlike many other peroxidases, a single enzyme rather than several isozymes are produced thus making more simple the purification and characterization of the enzyme. In this research it was shown that, as with other peroxidases, SBP is inactivated by azide during turnover. During azide oxidation SBP forms a ferrous-NO intermediate. In this respect, SBP is similar to the lignin peroxidases from Phanerochaete chrysosporium which also form ferrous-NO …

Silvopasture: An Agroforestry Practice, Ned B. Klopfenstein, W. J. Rietveld, Richard C. Carman, Terry R. Clason, Steven H. Sharrow, Gene Garrett, Bruce Anderson

Agroforestry Notes (USDA-NAC)

Although some form of silvopasture management has been practiced for centuries, silvopasture as an agroforestry practice is specifically designed and managed for the production of trees, tree products, forage, and livestock. Silvopasture results when forage crops are deliberately introduced or enhanced in a timber production system, or timber crops are deliberately introduced or enhanced in a forage production system. As a silvopasture, timber and pasture are managed as a single integrated system.

Silvopastoral systems are designed to produce a high-value timber component, while providing short-term cash flow from the livestock component. The interactions among timber, forage, and livestock are managed …

Forest Farming: An Agroforestry Practice, Mary Ellen Dix, Deborah B. Hill, Louise E. Buck, W. J. Rietveld

Agroforestry Notes (USDA-NAC)

Most public and private forest lands of North America have been modified to varying degrees from years of human activity. Certain high-value ‘non-timber forest products’ have been over-exploited and are difficult to find. Forest farming practices can be used by private enterprise to grow desirable non-timber forest products on private lands, to supplement family income, and to allow biodiversity to reestablish within forests.

Biología Silvopastoril, Steven H. Sharrow

Agroforestry Notes (USDA-NAC)

La producción forestal y la ganadería son dos usos de tierras comunes en muchos lugares del mundo que no tienen riego. El pastoreo forestal es una práctica tradicional en la mayor parte de América del Norte. Aproximadamente 25% del total de las tierras de los EE. UU., son pastoreadas. Los bosques jovenes y los bosques maduros con una copa o dosel poco denso, pueden proveer una cantidad considerable de forraje al ganado. Un itinerario de pastoreo hará más beneficioso el uso de la vegetación que crece bajo la copa de los árboles. De no ser así la vegetación competirá con …

The Biology Of Silvopastoralism, Steven H. Sharrow

Agroforestry Notes (USDA-NAC)

Forestry and livestock grazing are common competing uses for non-irrigated lands around the world. Forest grazing is a traditional practice in most of North America. Approximately 25 percent of all US forest land is grazed by livestock. Young forests and mature open-canopied forests can provide considerable amounts of forage for livestock. Prescription grazing makes beneficial use of ground vegetation that would otherwise compete with trees for soil moisture and nutrients, harbor gnawing rodents, and pose a fire hazard. Pasture grasses and legumes are sometimes seeded into newly harvested forest areas to reduce erosion, to provide high-quality food for deer and …

Growing Degree-Days: One Equation, Two Interpretations, Gregory S. Mcmaster, Wallace Wilhelm

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Heat units, expressed in growing degree-days (GDD), are frequently used to describe the timing of biological processes. The basic equation used is GDD = [(T_MAX + T_MIN)/2] - T_BASE where T_MAX and T_MIN are daily maximum and minimum air temperature, respectively, and T_BASE is the base temperature. Two methods of interpreting this equation for calculating GDD are: (1) if the daily mean temperature is less than the base, it is set equal to the base temperature, or (2) if T_MAX or T_MIN < T_BASE they are reset equal to T …

Field Evaluation Of Herbicides On Small Fruit, Vegetable, And Ornamental Crops, 1996, Ron Talbert, Lance A. Schmidt, Nilda R. Burgos, Jennifer A. Johnson, Joe K. Curless, Jason K. Norsworthy

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

The Prospect For An Asparagus Industry In Northern Western Australia, John Bonnardeaux

Horticulture published reports

Asparagus grown in temperate regions of southern Australia is available, fresh, between August and March. For the remainder of the year, asparagus is not grown in Australia on a significant scale.

Recent work in Thailand, Peru, Kununurra (northern-western Australia) and Katherine (Northern Territory) has indicated that good quality asparagus can be produced in tropical and subtropical climates. Furthermore, research has shown the timing of the harvest can be manipulated by withholding irrigation and fern cutting. In an environment such as the Ord River Irrigation Area (ORIA) it is possible to harvest asparagus at any time of the year.

This report …

Herbicide Evaluation In Arkansas Rice, 1996, Eric Webster, Ron Talbert, Ford Baldwin, David Gealy, Tomilea Dillon, Jason Norsworthy, Lance Schmidt, Dwayne Beaty

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

The Chemical Composition And Nutritive Value Of Australian Pulses, S. Sipas, J. B. Mackintosh, D. S. Petterson

Books & book chapters

Grain legumes are the harvested seed of leguminous crops, typically peas, beans and their close relatives within the Fabaceae. Another term for many of these crops is pulses. In Australia, generally grain legumes are referred to as pulses. The term pulse is derived from the Latin puls meaning that the seed or grain can be made into a thick soup or pottage. The term pulse is most commonly associated with the food legumes whereas grain legumes are mostly associated with the feed industry. The terms are interchangeable, but with few exceptions (notably chickpeas, lentils and mung beans), the majority of …

Conservation Compliance Credit For Winter Wheat Fall Biomass Production And Implications For Grain Yield, Gregory S. Mcmaster, Wallace Wilhelm

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Producers participating in federal farm and conservation programs must reduce potential erodibility below certain thresholds on lands classified as highly erodible. The Natural Resource Conservation Service (NRCS) will credit producer s in Colorado for the quantity of green winter wheat (Triticum aestivum L.) biomass at the beginning of the winter period towards compliance in reducing erosion. Unfortunately, few data exist on fall winter wheat biomass production and fall production varies widely based on many site-specific factors at planting and during the fall, and can be expensive to document. To address these problems, a crop simulation model called SHOOTGRO …

B.R. Wells Rice Research Studies 1996, R. J. Norman, T. H. Johnston

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

Proceedings Of The Global Conference On Ergot Of Sorghum, Carlos R. Casela, Jeffery A. Dahlberg

INTSORMIL Impacts and Bulletins

In June 1996, several of us had the opportunity to see sorghum ergot in Brazil and the damage that the disease can do. We saw severe damage in seed production plots and witnessed the pain that seed producers had to go through to deal with this new problem. There was a wide spread scare in the sorghum community in the Americas because of the infamous reputation of the disease in causing damage in seed production fields. As a silver lining, we also observed first hand, the excellent research program that the Brazilians had in place. It became apparent that all …

Bulletin 4328 - Soil Testing For Vegetable Production On The Coastal Plain, Ian Mcpharlin, Murray Hegney

Bulletins 4000 -

Vegetable growers need to use fertiliser efficiently. Under-fertilising can reduce yield, which results in economic losses. Over-fertilising can reduce yield and contribute to nitrogen and phosphorus pollution of ground and surface waters. Concerns have been expressed that fertilisers applied to horticultural crops contribute to the phosphorus pollution of the rivers and estuaries of the west and south coast of Western Australia.

Soil testing is a technique that can be used by growers to make better fertiliser decisions. When soil tests show high levels of nutrients, growers can reduce fertiliser application for the current crop. This should enable growers to maintain …

Pathogenicity Of Escherichia Coli O157:H7 In The Intestines Of Neonatal Calves, Evelyn A. Dean-Nystrom, Brad T. Bosworth, William C. Cray Jr., Harley W. Moon

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Cattle are an important reservoir of Shiga toxin-producing enterohemorrhagic Escherichia coli (EHEC) O157:H7 strains, foodborne pathogens that cause hemorrhagic colitis and hemolytic uremic syndrome in humans. EHEC O157:H7 strains are not pathogenic in calves >3 weeks old. Our objective was to determine if EHEC O157:H7 strains are pathogenic in neonatal calves. Calves(A/E) lesions in both the large and small intestines by 18 h postinoculation. The severity of diarrhea and inflammation, and also the frequency and extent of A/E lesions, increased by 3 days postinoculation. We conclude that EHEC O157:H7 strains are pathogenic in neonatal calves. The neonatal calf model is …

Proceedings Of The 24th Annual Meeting, Southern Soybean Disease Workers (March 15-16, 1997, Fort Walton Beach, Florida), Robert P. Mulrooney, Gabe Sciumbato, Steve Koenning, John Russin, Glenn G. Hammes

Southern Soybean Disease Workers: Conference Proceedings

Contents

Business session

Soybean Disease Loss Estimate for the Southern United States during 1996. Compiled by PW Pratt

Treasurer report. GG Hammes

SSDW Committee chairmen for 1996-1997

Graduate student papers

Characterization of Races of Phytophthora sojae in Arkansas and Their Effects on Commonly Grown Cultivars. TA Jackson, TL Kirkpatrick, and JC Rupe

Induction of Defense Related Proteins During Compatible and Incompatible Soybean-Cercospora sojina Interactions. WA Baker, J Qiu, CB Lawrence, S Tuzun, and DB Weaver

Contributed papers

Performance and Cyst Nematode Resistance of Selected Soybean Cultivars in North Carolina Production Fields. SR Koenning and KR Barker

Distribution and Diversity …

Bulletin 4333 - Furrow Sowing On Water Repellent Soils, Paul Blackwell, Grant Morrow

Bulletins 4000 -

This Bulletin discusses the benefits that furrow sowing brings to crop growth and production on water repellent sand, based on research and farm observations in Western Australian. By understanding the benefits and risks of several key processes, producers can better evaluate the potential role of furrow sowing in combating water repellency. This is true whether one is using direct drilling, no-till (narrow points or discs) or zero till (single, double or triple disc openers) methods.

A Guide To Mechanical Rangeland Regeneration, J. Addison

Bulletins 4000 -

The main objective of this Bulletin is to describe mechanical methods of promoting rangeland regeneration and how to determine which is the most appropriate to use on any particular site. Mechanical regeneration methods are not 'quick fix' but may be used to advantage in some rangeland regeneration strategies.

An Evaluation Of Mango (Mangifera Indica L.) Cultivars And Their Commercial Suitability For The Kimberley, P. R. Johnson, D. M. Robinson

Horticulture published reports

An evaluation of 74 mango cultivars was conducted from 1983 to 1995 in the Ord River Irrigation Area (ORIA) on Cockatoo sand. The aim to determine their suitability for commercial production and establish the basis for a mango industry in the Northern tropics of Western Australia. Measurements were made on yield, harvest times, flowering times, brix, fruit quality, growth rates and market acceptability. Results have indicated that cultivars, other than Kensington Pride, most commercially suitable in the ORIA are Banana for early cropping in mid September, Irwin for high regular yields and good market acceptability, Haden for excellent market acceptability, …

Riparian Buffers For Agricultural Land, Michael Dosskey, Richard C. Schultz, Thomas M. Isenhart

Agroforestry Notes (USDA-NAC)

Purpose:

• Define what a riparian buffer is

• Describe what benefits a riparian buffer can provide in an agricultural landscape

• Identify situations where installing a riparian buffer should be considered