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Articles 781 - 810 of 1074

Full-Text Articles in Medicinal Chemistry and Pharmaceutics

Bioactive Polyphenols In Kinkéliba Tea (Combretum Micranthum) And Their Glucose-Lowering Activities, C. Welch, J. Zhen, E. Bassène, I. Raskin, J.E. Simon, Q. Wu Jul 2020

Bioactive Polyphenols In Kinkéliba Tea (Combretum Micranthum) And Their Glucose-Lowering Activities, C. Welch, J. Zhen, E. Bassène, I. Raskin, J.E. Simon, Q. Wu

Journal of Food and Drug Analysis

Herbal tea kinkéliba prepared from the leaves of Combretum micranthum has been widely consumed in West African countries for its flavor, nutritional and medicinal properties. Under bio-guided screening, the kinkéliba leaves were chemically investigated using various chromatographic and spectrometric methods that led to the identification of thirteen different flavonoid compounds. Further biological tests illustrated that the identified compounds may have synergistic effects to decrease the expression of phosphoenolpyruvate carboxykinase (PEPCK) mRNA and glucose production in an H4IIE hepatoma cell line, indicating its potential use for insulin-resistant diabetes treatment. Further in vivo study on C57BL/6J mice indicates that kinkéliba can lower …

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Effect Of Drying Processes On Prenylflavonoid Content And Antioxidant Activity Of Epimedium Koreanum Nakai, Y. Zhao, S. Chen, Y. Wang, C. Lv, J. Wang, J. Lu Jul 2020

Effect Of Drying Processes On Prenylflavonoid Content And Antioxidant Activity Of Epimedium Koreanum Nakai, Y. Zhao, S. Chen, Y. Wang, C. Lv, J. Wang, J. Lu

Journal of Food and Drug Analysis

Epimedium koreanum Nakai is a famous Chinese herbal medicine for the treatment of impotence, osteoporosis, immune suppression and cardiovascular diseases. Drying is the most common and fundamental procedure in post-harvest processing of E. koreanum, which contributes to the variations of flavonoid content, especially prenylflavonoids, the bioactive components. In present study, effect of drying processes on flavonoid content and antioxidant activity were investigated. High performance liquid chromatography coupled with diode-array detection and electrospray ionization quadrupole time-of-flight tandem mass spectrometry methods were employed. Twenty seven compounds were identified and 11 of them, including eight prenylflavonoids and three other types of flavonoids, were …

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Thyme Essential Oils From Spain: Aromatic Profile Ascertained By Gc–Ms, And Their Antioxidant, Anti-Lipoxygenase And Antimicrobial Activities, A.-B. Cutillas, A. Carrasco, R. Martinez-Gutierrez, V. Tomas, J. Tudela Jul 2020

Thyme Essential Oils From Spain: Aromatic Profile Ascertained By Gc–Ms, And Their Antioxidant, Anti-Lipoxygenase And Antimicrobial Activities, A.-B. Cutillas, A. Carrasco, R. Martinez-Gutierrez, V. Tomas, J. Tudela

Journal of Food and Drug Analysis

Six samples of red thyme (Thymus zygis) and two samples of winter thyme (Thymus hyemalis) essential oils (EOs) were obtained from plants cultivated in south-eastern Spain and extracted by steam distillation. Analysis by gas chromatography coupled with mass spectrometry detection provided the relative (%) and absolute (mM) concentrations. Thymol (30–54%), p-cymene (14–27%) and γ-terpinene (8–28%) were the most abundant components of T. zygis EO, while 1,8-Cineole (3–37%), p-cymene (1–29%), linalool (8–13%) and thymol (0–19%) were the most abundant components in the case of T. hyemalis EO. Enantioselective gas chromatography identified (−)-linalool, (−)-borneol and (+)-limonene as the main enantiomers. Several methods …

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Employing Natural Reagents From Turmeric And Lime For Acetic Acid Determination In Vinegar Sample, S.-A. Supharoek, K. Ponhong, W. Siriangkhawut, K. Grudpan Jul 2020

Employing Natural Reagents From Turmeric And Lime For Acetic Acid Determination In Vinegar Sample, S.-A. Supharoek, K. Ponhong, W. Siriangkhawut, K. Grudpan

Journal of Food and Drug Analysis

A simple, rapid and environmentally friendly sequential injection analysis system employing natural extract reagents was developed for the determination of acetic acid following an acid–base reaction in the presence of an indicator. Powdered lime and turmeric were utilized as the natural base and indicator, respectively. Mixing lime and turmeric produced an orange to reddish-brown color solution which absorbed the maximum wavelength at 455 nm, with absorbance decreasing with increasing acetic acid concentration. Influential parameters including lime and turmeric concentrations, reagent and sample aspirated volumes, mixing coil length and dispensing flow rate were investigated and optimized. A standard calibration graph was …

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Use Of Uhplc-Tripleq With Synthetic Standards To Profile Anti-Inflammatory Hydroxycinnamic Acid Amides In Root Barks And Leaves Of Lycium Barbarum, S. Wang, J.H. Suh, W.-L. Hung, X. Zheng, Y. Wang, C.-T. Ho Jul 2020

Use Of Uhplc-Tripleq With Synthetic Standards To Profile Anti-Inflammatory Hydroxycinnamic Acid Amides In Root Barks And Leaves Of Lycium Barbarum, S. Wang, J.H. Suh, W.-L. Hung, X. Zheng, Y. Wang, C.-T. Ho

Journal of Food and Drug Analysis

Hydroxycinnamic acid amides (HCAA) are the secondary metabolites ubiquitously exist in flowering plants, formed by condensation between hydroxycinnamates and mono or polyamines. HCAA species not only serve multiple functions in plant growth and development, but also exert significant positive effects on human health. In this study, we combined organic synthesis and UPHLC-TripleQ-MS/MS specifically targeting at HCAA species. The method was fully validated with respect to specificity, linearity, intra- and inter-day precision and accuracy, limit of detection (LOD), limit of quantification (LOQ), recovery, and reproducibility. We applied this method to identify and quantify HCAAs from the root barks and leaves of …

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Stability-Indicating Spectrofluorimetric Method With Enhanced Sensitivity For Determination Of Vancomycin Hydrochloride In Pharmaceuticals And Spiked Human Plasma: Application To Degradation Kinetics, El-Din M.K. Sharaf, F. Ibrahim, A.K. El-Deen, K. Shimizu Jul 2020

Stability-Indicating Spectrofluorimetric Method With Enhanced Sensitivity For Determination Of Vancomycin Hydrochloride In Pharmaceuticals And Spiked Human Plasma: Application To Degradation Kinetics, El-Din M.K. Sharaf, F. Ibrahim, A.K. El-Deen, K. Shimizu

Journal of Food and Drug Analysis

Based on investigating the relative fluorescence intensity of vancomycin hydrochloride (VCM) in methanol, a simple, highly sensitive, time-saving and specific spectrofluorimetric method was developed and validated. VCM fluorescence was measured at 335 nm when excited at 268 nm. Excellent linearity is obeyed in the concentration range 1–100 ng/mL with a detection limit of 5.94 pg/mL, a quantitation limit of 18.03 pg/mL and a very good correlation coefficient (r = 0.9999). Our method was applied to analyze VCM in pharmaceuticals as well as spiked human plasma. Moreover, VCM stability was studied when exposed to various degradation conditions such as oxidative, alkaline …

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Cytotoxic Protobassic Acid Saponins From The Kernels Of Palaquium Formosanum, H.-Y. Chen, J.-F. Liu, S.-F. Tsai, Y.-L. Lin, S.-S. Lee Jul 2020

Cytotoxic Protobassic Acid Saponins From The Kernels Of Palaquium Formosanum, H.-Y. Chen, J.-F. Liu, S.-F. Tsai, Y.-L. Lin, S.-S. Lee

Journal of Food and Drug Analysis

Bioassay guided fractionation and separation of the EtOH extract of the kernels of Palaquium formosanum against PC-3 cells via Sephadex LH-20 and reverse phase C-18 columns led to the isolation of 13 protobassic saponins. One of these saponins is new and was characterized as 3‴-O-rhamnopyranosyl-arganin C, a bisdesmoside of 16α-hydroxyprotobassic acid at the C-3 and C-28 positions. The structures of these compounds were determined on the basis of 1D NMR ( 1 H, 13 C), 2D NMR ( 1 H– 1 H COSY, HSQC, HMBC, and NOESY), and selectively excited 1D TOCSY spectroscopic analyses and MS data, and comparison with …

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Gallic Acid Inhibits Bladder Cancer Cell Proliferation And Migration Via Regulating Fatty Acid Synthase (Fas), C.-C. Liao, S.-C. Chen, H.-P. Huang, C.-J. Wang Jul 2020

Gallic Acid Inhibits Bladder Cancer Cell Proliferation And Migration Via Regulating Fatty Acid Synthase (Fas), C.-C. Liao, S.-C. Chen, H.-P. Huang, C.-J. Wang

Journal of Food and Drug Analysis

Bladder cancer is known as the world's ninth most prevalent cancer in 2012. New cytotoxic drugs have created considerable progress in the treatment. Gallic acid (GA) has been shown to inhibit carcinogenesis in animal models and various cancer cell lines. The aim of the present study was to evaluate the effect of GA on proliferation and migration inhibition of a bladder cancer cell line. The results showed that GA inhibited fatty acid synthase (FAS) activity and increased ER alpha level of TSGH-8301 bladder cancer cell. GA regulated the cell proliferation via the PI3K/AKT and MAPK/ERK pathway. Immunoprecipitation assay demonstrated that …

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An Investigation Of The Olive Phenols Activity As A Natural Medicine, F. Gökalp Jul 2020

An Investigation Of The Olive Phenols Activity As A Natural Medicine, F. Gökalp

Journal of Food and Drug Analysis

The natural antioxidants of olive oil have phenolic structure and their activities are related to the formation of stable derivatives. In this study, the single components of the phenolic fraction of olive oil (1,4-hydroquinone, Semiquinone and 1,4-benzoquinone) have been studied as theoretical by using DFT (Density functional Theory). The behaviors of phenolic compounds of olive against to the alkyl peroxy radicals were investigated. Our data show that 1,4-benzoquinone is the best electron transfer agent in primary metabolic processes to human life. The frontier orbital gap, namely HOMO (highest occupied molecular orbital)–LUMO (lowest unoccupied molecular orbital) gap is the smallest for …

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A Simple And Robust Quantitative Analysis Of Retinol And Retinyl Palmitate Using A Liquid Chromatographic Isocratic Method, S. Yokota, S. Oshio Jul 2020

A Simple And Robust Quantitative Analysis Of Retinol And Retinyl Palmitate Using A Liquid Chromatographic Isocratic Method, S. Yokota, S. Oshio

Journal of Food and Drug Analysis

Vitamin A is a vital nutritional substances that regulates biological activities including development, but is also associated with disease onset. The extent of vitamin A intake influences the retinoid content in the liver, the most important organ for the storage of vitamin A. Measurement of endogenous retinoid in biological samples is important to understand retinoid homeostasis. Here we present a reliable, highly sensitive, and robust method for the quantification of retinol and retinyl palmitate using a reverse-phase HPLC/UV isocratic method. We determined the impact of chronic dietary vitamin A on retinoid levels in livers of mice fed an AIN-93G semi-purified …

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Antioxidant Activity And Calcium Binding Of Isomeric Hydroxybenzoates, Z. Zhao, M. Vavrusova, L.H. Skibsted Jul 2020

Antioxidant Activity And Calcium Binding Of Isomeric Hydroxybenzoates, Z. Zhao, M. Vavrusova, L.H. Skibsted

Journal of Food and Drug Analysis

The association constant for calcium binding to hydroxybenzoates in aqueous 0.16 M NaCl at 25 °C was found electrochemically to have the value K ass = 280 mol L −1 with ΔH o = −51 kJ mol −1 , ΔS o = −122 J mol −1 K −1 for the 2-isomer (salicylate), K ass = 7 mol L −1 with ΔH o = −39 kJ mol −1 , ΔS o = −116 J mol −1 K −1 for the 3-isomer, and K ass = 8 mol L −1 with ΔH o = −51 kJ mol −1 , ΔS o = …

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Systematic Study On Active Compounds As Antibacterial And Antibiofilm Agent In Aging Onions, K. Sharma, N. Mahato, Y.R. Lee Jul 2020

Systematic Study On Active Compounds As Antibacterial And Antibiofilm Agent In Aging Onions, K. Sharma, N. Mahato, Y.R. Lee

Journal of Food and Drug Analysis

Quantitative investigation and systematic studies of quercetin, total phenolics, flavonoids, antioxidants, antibacterial and antibiofilm or antibiofouling properties of methanolic extracts of onions obtained from six different varieties have been carried out to explore their relative merits in terms of biological activities of fresh and aging onions. Total phenolic content in the extracts was examined spectrophotometrically using Folin–Ciocalteau's phenol reagent and total antioxidant activity was studied by FRAP and DPPH methods. In vitro antibacterial activity of the extracts was investigated on Gram-negative (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus and Bacillus cereus) respectively, by using a modified Kirby–Bauer disc …

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Compositional Analyses Of White, Brown And Germinated Forms Of Popular Malaysian Rice To Offer Insight Into The Growing Diet-Related Diseases, B. Abubakar, H.M. Yakasai, N. Zawawi, M. Ismail Jul 2020

Compositional Analyses Of White, Brown And Germinated Forms Of Popular Malaysian Rice To Offer Insight Into The Growing Diet-Related Diseases, B. Abubakar, H.M. Yakasai, N. Zawawi, M. Ismail

Journal of Food and Drug Analysis

Diet-related metabolic diseases, and especially obesity, are metabolic disorders with multifactorial aetiologies. Diet has been a cornerstone in both the aetiology and management of this metabolic disorders. Rice, a staple food for over half of the world's population, could be exploited as part of the solution to check this menace which has been skyrocketing in the last decade. The present study investigated nine forms of rice from three widely grown Malaysian rice cultivars for in vitro and in vivo (glycaemic index and load) properties that could translate clinically into a lower predisposition to diet-related diseases. The germinated brown forms of …

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Effects Of Electrode Gap And Electric Current On Chlorine Generation Of Electrolyzed Deep Ocean Water, G.-S.W. Hsu, S.-Y. Hsu Jul 2020

Effects Of Electrode Gap And Electric Current On Chlorine Generation Of Electrolyzed Deep Ocean Water, G.-S.W. Hsu, S.-Y. Hsu

Journal of Food and Drug Analysis

Electrolyzed water is a sustainable disinfectant, which can comply with food safety regulations and is environmental friendly. A two-factor central composite design was adopted for studying the effects of electrode gap and electric current on chlorine generation efficiency of electrolyzed deep ocean water. Deep ocean water was electrolyzed in a glass electrolyzing cell equipped with platinum-plated titanium anode and cathode in a constant-current operation mode. Results showed that current density, chlorine concentration, and electrolyte temperature increased with electric current, while electric efficiency decreased with electric current and electrode gap. An electrode gap of less than 11.7 mm, and a low …

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Characterization Of Key Aroma-Active Compounds In Lychee (Litchi Chinensis Sonn.), S. Feng, M. Huang, J.H. Crane, Y. Wang Jul 2020

Characterization Of Key Aroma-Active Compounds In Lychee (Litchi Chinensis Sonn.), S. Feng, M. Huang, J.H. Crane, Y. Wang

Journal of Food and Drug Analysis

Volatile compounds in ‘Sweetheart’ lychee were examined using gas chromatography-olfactometry/mass spectrometry (GC-O/MS). Solvent assisted flavor evaporation (SAFE) technique was used to identify the aroma-active compounds in lychee. Further characterization of the most important odorants in ‘Sweetheart’ lychee was achieved using aroma extract dilution analysis (AEDA). Thirty-one key aroma-active odorants were identified in the flavor dilution (FD) factor range of 2–1024. Methional (cooked potato) and geraniol (sweet, floral) exhibited the highest FD factors of 1024 and 512, respectively, these were followed by furaneol (sweet, caramel), nerol (floral, sweet), dimethyl trisulfide (DMTS) (preserved vegetable, sulfury), linalool (floral), (E,Z)-2,6 nonadienal (cucumber) and nerolidol …

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Hptlc-Fld-Sers As A Facile And Reliable Screening Tool: Exemplarily Shown With Tyramine In Cheese, L. Wang, X.-M. Xu, Y.-S. Chen, J. Ren, Y.-T. Liu Jul 2020

Hptlc-Fld-Sers As A Facile And Reliable Screening Tool: Exemplarily Shown With Tyramine In Cheese, L. Wang, X.-M. Xu, Y.-S. Chen, J. Ren, Y.-T. Liu

Journal of Food and Drug Analysis

The serious cytotoxicity of tyramine attracted marked attention as it induced necrosis of human intestinal cells. This paper presented a novel and facile high performance thin-layer chromatography (HPTLC) method tailored for screening tyramine in cheese. Separation was performed on glass backed silica gel plates, using methanol/ethyl acetate/ammonia (6/4/1 v/v/v) as the mobile phase. Special efforts were focused on optimizing conditions (substrate preparation, laser wavelength, salt types and concentrations) of surface enhanced Raman spectroscopy (SERS) measurements directly on plates after derivatization, which enabled molecule-specific identification of targeted bands. In parallel, fluorescent densitometry (FLD) scanning at 380

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Water-Soluble Polysaccharides From Ulva Intestinalis: Molecular Properties, Structural Elucidation And Immunomodulatory Activities, M. Tabarsa, S. You, E.H. Dabaghian, U. Surayot Jul 2020

Water-Soluble Polysaccharides From Ulva Intestinalis: Molecular Properties, Structural Elucidation And Immunomodulatory Activities, M. Tabarsa, S. You, E.H. Dabaghian, U. Surayot

Journal of Food and Drug Analysis

Water-soluble sulfated polysaccharides extracted from Ulva intestinalis and fractionated using DEAE Sepharose fast flow column to identify their molecular properties and macrophage cells stimulating activities. Crude and fractions (F 1 and F 2 ) were formed of neutral sugars (58.7–74.7%), sulfates (6.2–24.5%), uronic acids (4.9–5.9%) and proteins (3.2–10.4%). Different levels of sugar constituents including rhamnose (30.1–39.1%), glucose (39.0–48.4%), galactose (0.0–15.8%), xylose (8.5–11.3) and arabinose (0.0–5.1%). The molecular weight (M w ) of crude and fractionated polysaccharides ranged from 87.1 × 10 3 to 194.1 × 10 3 (g/mol). Crude polysaccharides were not toxic to RAW264.7 cells and fractions induced cell …

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Relative Content Of Gallic Acid Over 5-Galloylquinic Acid As An Index For The Baking Intensity Of Oolong Teas, M.M.-C. Wang, Y. Yeh, Y.-E. Shih, J.T.-C. Tzen Jul 2020

Relative Content Of Gallic Acid Over 5-Galloylquinic Acid As An Index For The Baking Intensity Of Oolong Teas, M.M.-C. Wang, Y. Yeh, Y.-E. Shih, J.T.-C. Tzen

Journal of Food and Drug Analysis

Phenolic compounds in a series of old oolong teas prepared by baking annually were monitored and compared. The results showed that the relative content of gallic acid over 5-galloylquinic acid was subsequently elevated during this preparatory process. To reveal the effect was mainly resulted from baking or aging, two sets of oolong teas were collected and examined; one set was generated from fresh oolong tea via continually daily baking and the other set was composed of aged oolong teas with no or light baking in the storage period. The relative content of gallic acid over 5-galloylquinic acid was observed to …

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Production Of Antioxidant And Ace-Inhibitory Peptides From Kluyveromyces Marxianus Protein Hydrolysates: Purification And Molecular Docking, M. Mirzaei, S. Mirdamadi, M.R. Ehsani, M. Aminlari Jul 2020

Production Of Antioxidant And Ace-Inhibitory Peptides From Kluyveromyces Marxianus Protein Hydrolysates: Purification And Molecular Docking, M. Mirzaei, S. Mirdamadi, M.R. Ehsani, M. Aminlari

Journal of Food and Drug Analysis

Kluyveromyces marxianus protein hydrolysates were prepared by two different sonicated-enzymatic (trypsin and chymotrypsin) hydrolysis treatments to obtain antioxidant and ACE-inhibitory peptides. Trypsin and chymotrypsin hydrolysates obtained by 5 h, exhibited the highest antioxidant and ACE-inhibitory activities. After fractionation using ultrafiltration and reverse phase high performance liquid chromatography (RP-HPLC) techniques, two new peptides were identified. One fragment (LL-9, MW = 1180 Da) with the amino acid sequence of Leu-Pro-Glu-Ser-Val-His-Leu-Asp-Lys showed significant ACE inhibitory activity (IC 50 = 22.88 μM) while another peptide fragment (VL-9, MW = 1118 Da) with the amino acid sequence of Val-Leu-Ser-Thr-Ser-Phe-Pro-Pro-Lys showed the highest antioxidant and ACE …

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Exploration Of Wedelia Chinensis Leaf-Assisted Silver Nanoparticles For Antioxidant, Antibacterial And In Vitro Cytotoxic Applications, Das M. Paul, Livingstone J. Rebecca, P. Veluswamy, J. Das Jul 2020

Exploration Of Wedelia Chinensis Leaf-Assisted Silver Nanoparticles For Antioxidant, Antibacterial And In Vitro Cytotoxic Applications, Das M. Paul, Livingstone J. Rebecca, P. Veluswamy, J. Das

Journal of Food and Drug Analysis

Green synthetic route of silver nanoparticles (AgNPs) has already been proved to be an advantageous over other physico-chemical approaches due to its simplicity, cost effectiveness, ecofriendly and nontoxicity. In this finding, aqueous Wedelia chinensis leaf extract (WLE) mediated synthesis of AgNPs was approached. Surface plasmon resonance (SPR) band at 408 nm preliminary indicated the formation of AgNPs, while TEM and XRD characterization confirmed the formation of spherically shaped and crystalline AgNPs with an average size of 31.68 nm, respectively. The plausible biomolecules in the aqueous leaf extract responsible for the reduction and stabilization of AgNPs were identified by FTIR analysis …

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Characteristics Of Fucose-Containing Polysaccharides From Submerged Fermentation Of Agaricus Blazei Murill, H.-T. Wang, L.-C. Yang, H.-C. Yu, M.-L. Chen, H.-J. Wang, T.-J. Lu Jul 2020

Characteristics Of Fucose-Containing Polysaccharides From Submerged Fermentation Of Agaricus Blazei Murill, H.-T. Wang, L.-C. Yang, H.-C. Yu, M.-L. Chen, H.-J. Wang, T.-J. Lu

Journal of Food and Drug Analysis

Fucose is one of important residues of recognition pattern for many immune cells. In this study, we characterized bioactive fucose-containing acidic polysaccharides from submerged fermentation of Agaricus blazei Murill. We obtained the polysaccharides through a cell-based activity-guided strategy, and used carbohydrate recognition monoclonal antibodies based Enzyme-Linked Immuno Sorbent Assay (ELISA) along with methylation and NMR analyses to investigate the structural characteristics of the polysaccharides. The polysaccharides had Mw of 3.5 × 10 5 Da. The major sugars were L-fucose, L-arabinose, D-galactose, D-xylose, and D-galacturonic acid in the molar ratio of 6.4, 15.5, 28.5, 14.7, and 25.0% with a small amount …

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Determination Of Total Mercury In Seafood By Ion-Selective Electrodes Based On A Thiol Functionalized Ionic Liquid, J. Miao, X. Wang, Y. Fan, J. Li, L. Zhang, G. Hu, C. He, C. Jin Jul 2020

Determination Of Total Mercury In Seafood By Ion-Selective Electrodes Based On A Thiol Functionalized Ionic Liquid, J. Miao, X. Wang, Y. Fan, J. Li, L. Zhang, G. Hu, C. He, C. Jin

Journal of Food and Drug Analysis

A mercury(II) ion-selective electrode with an ionic liquid (IL), 1-methyl-2-butylthioimidazolium bis(trifluoromethanesulphonyl)imide ([C 1 C 4 Sim]NTf 2 ) as active material was constructed. Parameters affecting the performance of the electrodes such as the dosages of the IL and carbon nanotubes and the aqueous pH values were investigated. Experimental results indicated that the optimal composition of the electrode filling material was 47.6% [C 1 C 4 Sim]NTf 2 , 47.6% tetrabutylphosphonium bis(trifluoromethanesulphonyl)imide (TBPNTf 2 ) and 4.8% carboxylic multi-walled carbon nanotubes (MWCNTs-COOH). Under the selected conditions, the proposed electrodes showed a good linear response in the concentration range of 10 −10 …

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Analysis Of Veterinary Drug Residue Monitoring Results For Commercial Livestock Products In Taiwan Between 2011 And 2015, H.-C. Lee, C.-M. Chen, J.-T. Wei, H.-Y. Chiu Jul 2020

Analysis Of Veterinary Drug Residue Monitoring Results For Commercial Livestock Products In Taiwan Between 2011 And 2015, H.-C. Lee, C.-M. Chen, J.-T. Wei, H.-Y. Chiu

Journal of Food and Drug Analysis

Antibiotics have been widely used in the treatment of livestock diseases. However, the emergence of issues related to drug resistance prompted governments to enact a series of laws regulating the use of antibiotics in livestock. Following control of the problem of drug resistant bacteria, public attention has shifted to the recurring incidence of human health and safety issues caused by residual veterinary drugs in livestock products. To guarantee the safety and hygiene of meat, milk, and eggs from food-producing animals, governments and relevant agencies established laws and regulations for the use of veterinary drugs. It is, therefore, necessary to monitor …

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A Validated Capillary Electrophoretic Method For The Determination Of Indacaterol And Its Application To A Pharmaceutical Preparation, T. Güray, M. Tunçel, U.D. Uysal Jul 2020

A Validated Capillary Electrophoretic Method For The Determination Of Indacaterol And Its Application To A Pharmaceutical Preparation, T. Güray, M. Tunçel, U.D. Uysal

Journal of Food and Drug Analysis

Indacaterol is a new inhaled ultra-long acting β 2 -agonist. It has been recently approved in the European Union for the treatment of chronic obstructive pulmonary disease. This paper reports, for the first time, a method for the determination and validation of Indacaterol (IND) using an internal standard in capsules. Capillary electrophoretic separation was performed on an uncoated fused-silica capillary (50 cm effective length, 75 μm i.d.) and background electrolyte composed of 20 mmol L −1 of sodium tetraborate buffer, 15% (v/v) methanol (pH = 10.0) with the application of 20 kV of potential; 10 s at 5 × 10 …

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Sialoglycoprotein Isolated From Eggs Of Carassius Auratus Promotes Fracture Healing In Osteoporotic Mice, F. Wang, L. Han, X. Wang, Y. Li, Y. Zhu, J. Wang, C. Xue Jul 2020

Sialoglycoprotein Isolated From Eggs Of Carassius Auratus Promotes Fracture Healing In Osteoporotic Mice, F. Wang, L. Han, X. Wang, Y. Li, Y. Zhu, J. Wang, C. Xue

Journal of Food and Drug Analysis

In this study, open tibial fracture surgery was performed on mice with ovariectomy induced osteoporosis to investigate the effect of a treatment with sialoglycoprotein isolated from Carassius auratus eggs (Ca-SGP) on fracture healing. Dynamic histological analysis showed that Ca-SGP promoted the generation of cartilage callus on day 5 post-surgery, then facilitated the transformation of the cartilage callus to bony callus on days 11 and 24 post-surgery, and enhanced the remodeling of bony callus on 35 day post-surgery. Moreover, Ca-SGP significantly decreased the secretion of TNF-α and IL-1β in serum on day 5 post-surgery, thus inhibiting the negative spread of the …

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Pharmacokinetics, Bioavailability, Tissue Distribution And Excretion Of Tangeretin In Rat, W.-L. Hung, W.-S. Chang, W.-C. Lu, G.-J. Wei, Y. Wang, C.-T. Ho, L.S. Hwang Jul 2020

Pharmacokinetics, Bioavailability, Tissue Distribution And Excretion Of Tangeretin In Rat, W.-L. Hung, W.-S. Chang, W.-C. Lu, G.-J. Wei, Y. Wang, C.-T. Ho, L.S. Hwang

Journal of Food and Drug Analysis

Tangeretin, 4′,5,6,7,8-pentamethoxyflavone, is one of the major polymethoxyflavones (PMFs) existing in citrus fruits, particularly in the peels of sweet oranges and mandarins. Tangeretin has been reported to possess several beneficial bioactivities including anti-inflammatory, anti-proliferative and neuroprotective effects. To achieve a thorough understanding of the biological actions of tangeretin in vivo, our current study is designed to investigate the pharmacokinetics, bioavailability, distribution and excretion of tangeretin in rats. After oral administration of 50 mg/kg bw tangeretin to rats, the C max , T max and t 1/2 were 0.87 ± 0.33 μg/mL, 340.00 ± 48.99 min and 342.43 ± 71.27 min, …

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Methodology For Sample Preparation And Size Measurement Of Commercial Zno Nanoparticles, P.-J. Lu, W.-E. Fu, S.-C. Huang, C.-Y. Lin, M.-L. Ho, Y.-P. Chen, H.-F. Cheng Jul 2020

Methodology For Sample Preparation And Size Measurement Of Commercial Zno Nanoparticles, P.-J. Lu, W.-E. Fu, S.-C. Huang, C.-Y. Lin, M.-L. Ho, Y.-P. Chen, H.-F. Cheng

Journal of Food and Drug Analysis

This study discusses the strategies on sample preparation to acquire images with sufficient quality for size characterization by scanning electron microscope (SEM) using two commercial ZnO nanoparticles of different surface properties as a demonstration. The central idea is that micrometer sized aggregates of ZnO in powdered forms need to firstly be broken down to nanosized particles through an appropriate process to generate nanoparticle dispersion before being deposited on a flat surface for SEM observation. Analytical tools such as contact angle, dynamic light scattering and zeta potential have been utilized to optimize the procedure for sample preparation and to check the …

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Standards And Labeling Of Milk Fat And Spread Products In Different Countries, C.-L. Lee, H.-L. Liao, W.-C. Lee, C.-K. Hsu, F.-C. Hsueh, J.-Q. Pan, C.-H. Chu, C.-T. Wei, M.-J. Chen Jul 2020

Standards And Labeling Of Milk Fat And Spread Products In Different Countries, C.-L. Lee, H.-L. Liao, W.-C. Lee, C.-K. Hsu, F.-C. Hsueh, J.-Q. Pan, C.-H. Chu, C.-T. Wei, M.-J. Chen

Journal of Food and Drug Analysis

In the present paper, we provide comprehensive information related to labeling claims and standards of identity of milk fat and spread products. By reviewing the standards and regulations for dairy and non-dairy fat products, Codex Alimentarius and several other countries have clearly specified these products and set the requirements for use of the product name to prevent misleading or confusing the consumers. Generally, for the milkfat products, the fat in the products should be exclusively from milk and/or the products obtained from milk. The milkfat contents of these products should be no less than 10%. Whereas, the blends or blended …

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Determination Of Ractopamine And Salbutamol In Pig Hair By Liquid Chromatography Tandem Mass Spectrometry, K.-C. Chang, Y.-T. Chang, C.-E. Tsai Jul 2020

Determination Of Ractopamine And Salbutamol In Pig Hair By Liquid Chromatography Tandem Mass Spectrometry, K.-C. Chang, Y.-T. Chang, C.-E. Tsai

Journal of Food and Drug Analysis

A liquid chromatography tandem mass spectrometric method was developed for the determination of two β-agonists (ractopamine and salbutamol) in pig hair samples. An isotope of ractopamine-d5 or salbutamol-d6 as an internal standard was used to carry out quantitative analysis. Concentrated sodium hydroxide was used to pretreat hair samples and then purified by the solid phase extraction (SPE) procedure. The extracted solution was evaporated and reconstituted for injection in the instrument with electrospray ionization (ESI) operating in a positive multiple-reaction-monitoring (MRM) mode. Ractopamine and salbutamol separation were performed on C18 analytical column under gradient condition. The internal standard calibration curve was …

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The Integration Of Multi-Platform Ms-Based Metabolomics And Multivariate Analysis For The Geographical Origin Discrimination Of Oryza Sativa L., D.K. Lim, C. Mo, J.H. Lee, N.P. Long, Z. Dong, J. Li, J. Lim, S.W. Kwon Jul 2020

The Integration Of Multi-Platform Ms-Based Metabolomics And Multivariate Analysis For The Geographical Origin Discrimination Of Oryza Sativa L., D.K. Lim, C. Mo, J.H. Lee, N.P. Long, Z. Dong, J. Li, J. Lim, S.W. Kwon

Journal of Food and Drug Analysis

For the authentication of white rice from different geographical origins, the selection of outstanding discrimination markers is essential. In this study, 80 commercial white rice samples were collected from local markets of Korea and China and discriminated by mass spectrometry-based untargeted metabolomics approaches. Additionally, the potential markers that belong to sugars & sugar alcohols, fatty acids, and phospholipids were examined using several multivariate analyses to measure their discrimination efficiencies. Unsupervised analyses, including principal component analysis and k-means clustering demonstrated the potential of the geographical classification of white rice between Korea and China by fatty acids and phospholipids. In addition, the …

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