Pharmacology, Toxicology and Environmental Health Commons^™

Indomethacin Reduces Splenic Red Pulp Macrophage Populations In Female New Zealand White Rabbits, Thane S. Thurmond

Electronic Theses and Dissertations

In an effort to elucidate the mechanism by which indomethacin (IN) attenuates the stimulatory effect of estradiol (E$\sb2$) on rabbit splenic red pulp macrophages (RPM), thirty-nine female New Zealand White rabbits were divided into 10 groups: ovariectomized (OVX), OVX/IN at 0.1 and 5.0 mg/kg body weight (bw)/day; sham OVX (SOVX), SOVX/IN at 0.1 and 5.0 mg/kg bw/day; OVX/25 mg E2, OVX/25 mg E$\sb2$/IN at 0.1 and 5.0 mg/kg bw/day; intact Control. Quantitative changes in RPM population in response to the treatments were measured using a 0 to 4 histologic grading scale. Estradiol treatment resulted in increased RPM grade when compared …

Altered Platelet Activating Factor Metabolism In Insulin Dependent Diabetes Mellitus, R A. Memon, S A. Saeed, A Jabbar, A Jafri, A H. Gilani, S Saleem, H Akbani

Department of Biological & Biomedical Sciences

Diabetes mellitus is associated with several abnormalities of platelet function. Recent studies have shown that the blood level of platelet activating factor (PAF), a potent inducer of platelet aggregation, is elevated in insulin dependent diabetes mellitus (IDDM) and remains unchanged in non-insulin dependent diabetes mellitus (NIDDM) patients. However, the mechanism of this increase in PAF levels has not been determined. In this study we have measured the activity of plasma PAF acetylhydrolase (an enzyme that regulates PAF levels) and lipoprotein levels in control subjects and diabetic patients. The data presented show that plasma PAF acetylhydrolase activity is significantly decreased in …

การเรียนการสอนเภสัชจลนศาสตร์ จุฬาลงกรณ์มหาวิทยาลัย, เพ็ญศรี ทองนพเนื้อ

The Thai Journal of Pharmaceutical Sciences

No abstract provided.

Comparison Of Piroxicam Flux From Gel Preparations Through Polydimethylsiloxane Membrane And Pig Skin, Panida Vayumhasuwan, Busaba Polpakdee

The Thai Journal of Pharmaceutical Sciences

In vitro permeation behaviors of a synthetic membrane, Silastic™, and a biological membrane, newborn pig skin, were characterized using piroxicam as a model drug. Experiments had been carried out using modified Franz diffusion cells. Various gel bases containing 2% piroxicam were prepared to study the in vitro permeation of the drug. Gelling agents studied were Pluronic F127, Carbopol 940, hydroxypropyl methylcellulose and hydroxyethyl cellulose. Piroxicam fluxes from the four gel bases through SilasticT were correlated with the fluxes through pig skin with the r2 of 0.96. The Carbopol 940 gel base yielded the highest flux. It was, therefore, used for …

การเรียนการสอนเภสัชจลนศาสตร์ จุฬาลงกรณ์มหาวิทยาลัย, เพ็ญศรี ทองนพเนื้อ

The Thai Journal of Pharmaceutical Sciences

No abstract provided.

Comparison Of Piroxicam Flux From Gel Preparations Through Polydimethylsiloxane Membrane And Pig Skin, Panida Vayumhasuwan, Busaba Polpakdee

The Thai Journal of Pharmaceutical Sciences

In vitro permeation behaviors of a synthetic membrane, Silastic™, and a biological membrane, newborn pig skin, were characterized using piroxicam as a model drug. Experiments had been carried out using modified Franz diffusion cells. Various gel bases containing 2% piroxicam were prepared to study the in vitro permeation of the drug. Gelling agents studied were Pluronic F127, Carbopol 940, hydroxypropyl methylcellulose and hydroxyethyl cellulose. Piroxicam fluxes from the four gel bases through SilasticT were correlated with the fluxes through pig skin with the r2 of 0.96. The Carbopol 940 gel base yielded the highest flux. It was, therefore, used for …

Development Of A Stable Topical Indomethacin Solution, Panida Vayumhasuwan, Saranya Tharasawaeng

The Thai Journal of Pharmaceutical Sciences

Each topical indomethacin solution formulated was composed of ethanol, propylene glycol, a surfactant and water. The surfactants studied were polysorbate 80, pluronic F127 and pluronic F68. Predicted shelf lives at an average laboratory room temperature (33 ℃) of the formulations containing 5% polysorbate 80, 5% pluronic F127 and 5% pluronic F68 were 8.23, 7.43 and 5.06 years, respectively. From a statistical point of view, first order rate constants predicted by the Arrhenius equation were not different from those actually obtained at ambient temperature.

เทคโนโลยีทางเภสัชกรรม ตอนที่ 1, ไกรสีห์ อัมพรายน์

The Thai Journal of Pharmaceutical Sciences

No abstract provided.

Development Of Methyl Salicylate Preparations Sontaining Herbal Extracts For Relief Of Local Skin Inflammation. Formulation And In Vivo Evalauation Of Gel And Ointment Dosage Forms In Mice, Parkpoom Tengamnuay, Withaya Janthasoot, Veeraya Rattanachaipipat, Wallapa Rattanachaipipat

The Thai Journal of Pharmaceutical Sciences

Topical methyl salicylate (MS) ointments or analgesic balms have long been used for treatment of minor skin irritations and muscle pains. The products generally contain 15- 50% w/w of MS in an appropriate ointment base. Their pain-relieving action is mainly a result of the counterirritant and rubefacient effects of MS and menthol contained in these preparations. This study attempted to modify the conventional MS ointment in order to enhance its efficacy by including some herbal ingredients with known antiinflammatory/antiedema activities in the formulation. Aescin, chamomile and aloe vera gel extracts were the three natural ingredients selected for inclusion. The modified …

เทคโนโลยีทางเภสัชกรรม ตอนที่ 2, ไกรสีห์ อัมพรายน์

The Thai Journal of Pharmaceutical Sciences

No abstract provided.

Spray Dried Rice Starch For Direct Compression.(แป้งข้าวเจ้าชนิดพ่นแห้งสำหรับใช้ในการตอกยาเม็ดโดยตรง), Ampol Mitrevej, Nuttanan Sinchaipanid, Surachai Palanuphap

The Thai Journal of Pharmaceutical Sciences

A direct compression filler (SDRS) was prepared by spray drying rice starch. Scanning electron microscope revealed that a particle of SDRS was made up entirely of aggregates of rice starch grains, and the shape of SDRS was extremely spherical. Because of...

Phospholipid Microemulsions: Ii. Electronphotomicrographs Of Systems Containing Butanol As A Co-Surfactant.(ฟอสโฟไลปีดไมโครอิมัลชัน: Ii. ภาพถ่ายจากกล้องจุลทรรศน..., Wanchai Sutananta

The Thai Journal of Pharmaceutical Sciences

The electronphotomicrographs of phospholipid microemulsions containing butanol as a co-surfactant were presented. Various structures were found. For example, a definite oil core or a hard sphere type microemulsion was found in a sample containing isoprop...

Subject Index, Author Index(ดัชนีคำสำคัญ, ดัชนีผู้แต่ง), N/A

The Thai Journal of Pharmaceutical Sciences

No abstract provided.

เทคโนโลยีทางเภสัชกรรม ตอนที่ 1, ไกรสีห์ อัมพรายน์

The Thai Journal of Pharmaceutical Sciences

No abstract provided.

The Application Of Powdered Sugar-Tapioca Starch Granules Prepared By Fluidized Bed Granulation Technique In Direct Tableting Process, Poj Kulvanich, Detpon Prechagoon

The Thai Journal of Pharmaceutical Sciences

This report is concerned with the production of granules from powdered sugar-tapioca starch mixtures by fluidized bed granulation technique. The application of such granules as direct compression vehicles in manufacture of tablets products was demonstrated. The mixtures of powdered sugar-tapioca starch in the ratio of 1:1 were employed to prepare the granules using polyvinylpyrrolidone and gelatin as the granulating agents. Successful granulation of sugar-starch mixtures in a fluidized bed granulator were attained to have porous, irregular shape granules with narrow size distribution and low density. The physical properties of granular products using two binders were different. The finished granules were …

พิษของยาสมุนไพรตีสาร (แก้ปิตตะสมุฏฐาน), ปราณี ชวลิตธำรง, เอมมนัส อัตตวิชญ์, พัช รักษามั่น, ปราณี จันทเพ็ชร

The Thai Journal of Pharmaceutical Sciences

ยาแผนโบราณตรีสารประกอบด้วยสมุนไพร 3 ชนิดคือ รากเจตมูลเพลิงแดง รากช้าพลู และเถาสะค้านในอัตราส่วนที่ต่างกันแล้วแต่สมุฎฐาน (ปิตตะ วาตะ หรือเสมหะ) จากการศึกษาพิษเฉียบพลันของสารสกัดด้วย 50% เอธานอลของสมุนไพรที่เป็นส่วนผสมของยาแผนโบราณตรีสารในหนูถีบจักรพบว่า สารสกัด เจตมูลเพลิงแดงเมื่อให้ทางปาก และฉีดเข้าใต้ผิวหนัง มีค่า LD เทียบเท่าผงยาแห้งเท่ากับ 23.2 และ 17.8 ก./กก. ตามลำดับ ส่วนสารสกัดสะค้านและสารสกัดข้าพลูเมื่อให้ทางปากหรือฉีดเข้าใต้ผิวหนัง มีค่า LD มากกว่า 40.0 ก./กก. และจากการศึกษาพิษกึ่งเฉียบพลันของยาแผนโบราณตรีสารแก้ปิตตะสมุฏฐาน(สำหรับรักษาอาการป่วยด้วยธาตุไฟในฤดูหนาว)ซึ่งประกอบด้วยรากเจตมูลเพลิงแดง 12 ส่วน รากช้าพลู 4 ส่วน และเถ้าสะค้าน 8 ส่วน ในหนูขาวพันธุ์วิสตาร์ โดยป้อนยาในขนาด 0.36, 2.88 และ 23.04 ก./กก./วัน เป็นระยะเวลา 10 วันหรือคิดเป็น 1, 8 และ 64 เท่าของขนาดที่ใช้ในคน พบว่าในหนูทั้งสองเพศทุกกลุ่มที่ได้รับยามีการเจริญเติบโต และค่าทางโลหิตวิทยาคือค่า ฮีมาโตคริต ค่าจำนวนเม็ดเลือดขาว ค่าจำนวนเกล็ดเลือด ไม่แตกต่างจากกลุ่มควบคุม ส่วนค่าทางชีวเคมีของหนูขาวทุกกลุ่มที่ได้รับยาพบว่ามีค่าครีอาตินินสูงกว่ากลุ่มควบคุมอย่างมีนัยสำคัญ จากการตรวจพยาธิสภาพภายนอกของ อวัยวะต่าง และจุลพยาธิวิทยาของตับ และไต ไม่พบความผิดปกติที่เกิดจากยาตรีสารแก้ปิตตะสมุฏฐาน

Flavonoidal Constituents And Pharmacological Activities Of Glycyrrhiza Species, Shi-Shan Jia, Gui-Fen Qiu, Yuichi Fujii, Fumio Ikegam, Isamu Marakoshi

The Thai Journal of Pharmaceutical Sciences

This review deals with the similarities and differences of flavonoidal constituents isolated from the different parts of several Glycvrrhiza species (licorice plant), including the root and rhizome and the aerial part, with their chemotaxonomic character...

Corydaldine From Stephania Pierrei, Kittisak Likhitwitayawuid, Nijsiri Ruangrungsi

The Thai Journal of Pharmaceutical Sciences

Corydaldine [1] was isolated from the tubers of Stephania pierrei Diels. The complete 3H and unambiguous 13c NMR assignments of 1 were obtained through extensive NMR studies.

Antimicrobial Activity Of Cephalosporins Against Pathogenic Gram-Negative Bacilli(ฤทธิ์ต้านจุลชีพของ Cephalosporins ที่มีต่อเชื้อก่อโรครูปแท่งแกรมลบ), Mali Wirotesangthong, Pintip Pongpech

The Thai Journal of Pharmaceutical Sciences

Antimicrobial activity of cephalosporins against 241 gram-negative bacterial strains isolated from clinical specimens at Siriraj Hospital was determined. The majority (83.3-100%) of all strains of E. coll, Vibrio spp., Proteus spp. and Salmonella spp. we...

Theophylline Sustained Release Tablets Prepared From Theophylline Granules Coated With Ethylcellulose Using Fluidized Bed Technique(ยาเม็ดธืโอฟิลินชนิดออกฤทธิ์นานซึ่งเตรียม..., Poj Kulvanich, Sudarat Eiamtrakarn

The Thai Journal of Pharmaceutical Sciences

Theophylline granular cores were prepared from different fillers (microcrystalline cellulose, corn starch, dibasic calcium phosphate and lactose) by wet granulation. The theophylline granules were then coated with ethylcellulose using fluidized bed coati...

การอบรม Effective Drug Management And Rational Drug Use, พัชรินทร์ สุวรรณกูฏ

The Thai Journal of Pharmaceutical Sciences

No abstract provided.

1h And 13c Nmr Assignments Of Eleutherol, Kittisak Likhitwitayawuid, Nijsiri Ruangrungsi, Rawiwun Kaewamatawong

The Thai Journal of Pharmaceutical Sciences

Complete ]H and unequivocal 13c NMR assignments of eleutherol [1] were obtained through extensive NMR studies, including homonuclear COSY, NOE difference, HETCOR and HMBC experiments.

Phase Ii Trial Of Murine Monoclonal Antibody D612 Combined With Recombinant Human Monocyte Colony-Stimulating Factor (Rhm-Csf) In Patients With Metastatic Gastrointestinal Cancer, Mansoor Saleh, Michael Khazaeli, Richard Wheeler, Pat Bucy, Tiepe Liu, Michael Everson, David Mun, Jeff Schlom

Haematology and Oncology, East Africa

In a Phase II study, 14 patients with metastatic gastrointestinal cancer received the mAb D612 (40 mg/m2, days 4, 7, and 11) in combination with recombinant human monocyte colony-stimulating factor [(rhM-CSF) 80 ug/kg/days 1-14]. The combined treatment was well tolerated and resulted in characteristic biological activity associated with each of the agents. Thus, 10 of 14 patients experienced D612-associated secretory diarrhea, which responded to the prostaglandin inhibitor Indomethacin in 5 of 7 patients. rhM-CSF therapy was associated with peripheral mono- cytosis (peak absolute monocyte count, 1444 ±394/mm3) and thrombocytopenia (nadir count, 78 ±10/nim '). Monocyte surface marker analysis revealed a …

Development Of A Stable Topical Indomethacin Solution, Panida Vayumhasuwan, Saranya Tharasawaeng

The Thai Journal of Pharmaceutical Sciences

Each topical indomethacin solution formulated was composed of ethanol, propylene glycol, a surfactant and water. The surfactants studied were polysorbate 80, pluronic F127 and pluronic F68. Predicted shelf lives at an average laboratory room temperature (33 ℃) of the formulations containing 5% polysorbate 80, 5% pluronic F127 and 5% pluronic F68 were 8.23, 7.43 and 5.06 years, respectively. From a statistical point of view, first order rate constants predicted by the Arrhenius equation were not different from those actually obtained at ambient temperature.

Cd16+ Monocytes In Patients With Cancer: Spontaneous Elevation And Pharmacologic Induction By Recombinant Human Macrophage Colony- Stimulating Factor, Mansoor Saleh, Goldman Samuel, Lobuglio Albert, Beall Arthur, Sabio Hernan, Mccord Melissa, Minasian Lori, Alpaugh Katherine, Weiner Louis, Munn David

Haematology and Oncology, East Africa

The small subset of circulating monocytes that express the maturation- associated CD16 antigen has recently been reported to be elevated in patients with bacterial sepsis. We now show that this novel CD16+ monocyte population is also spontaneously expanded in patients with cancer. We studied 14 patients with metastatic gastrointestinal carcinoma enrolled in a clinical trial of recombinant human macrophage colony-stimulating factor (rhMCSF) plus monoclonal antibody D612. We found that before any cytokine treatment, 12 of 14 patients constitutively displayed significant elevations in both the percentage and the absolute number of CD16+ monocytes, as compared with both normal subjects and ill …

In Situ Regulation Of Cytosolic Phospolipase A₂, Beverly A. Rzigalinski

Theses and Dissertations in Biomedical Sciences

The 85 kDa cytosolic phospholipase A₂ (cPLA₂) is an agonist-responsive effector for intracellular signal transduction through the arachidonate cascade. In vitro studies have demonstrated that this enzyme is regulated by sub-micromolar calcium and is specific for arachidonate as the sn-2 fatty acyl group of phospholipid substrates. However, very little data is available regarding in situ mechanisms which govern the activity of cPLA₂. The primarily objective of these studies was to develop an in situ system for the study of cPLA₂, and investigate mobilization of arachidonate during signal transduction events.

Dimethylsulfoxide differentiation of the …

Cerebral Microcirculatory Effects Of Maturation, Scott R. Elliott

Loma Linda University Electronic Theses, Dissertations & Projects

The present studies demonstrate that in the second (2B) and fourth (4B) branches of newborn and adult sheep middle cerebral arteries, 5-HT2 serotonergic receptors mediate contractile responses to serotonin and that alpha-1 adrenergic receptors mediate contractile responses to norepinephrine. In addition, tissue sensitivity to 5-HT and NE decreases significantly with maturation, but does not vary with branch order. These age-related changes were associated with a decrease in affinity and maximum response in 2B and 4B segments of 5-HT and NE contracted arteries, respectively. Norepinephrine and 5-HT occupancy at the pD₂ rose significantly in 2B segments, but did not change …

Antithrombotic Activity Of Clove Oil, Shaikh A. Saeed, A H. Gilani

Department of Biological & Biomedical Sciences

Clove oil inhibited human platelet aggregation induced by arachidonic acid (AA), platelet-activating factor (PAF) or collagen. Clove oil was a more effective inhibitor for aggregation induced by AA and PAF (IC50:4 and 6 microM respectively) than collagen (IC50: 132 microM). The in vivo experiments in rabbits showed that clove oil (50-100 mg/kg) afforded 100% protection against PAF (11 mg/kg, i.v.) and 70% protection against AA (2.0 mg/kg, i.v.)-induced thrombosis and shock due to pulmonary platelet thrombosis. It also inhibited thromboxane-A2 and 12-HETE production by human platelets incubated with [C14] AA. These results are indicative that clove oil is inhibitory of …

Characterization Of The Vasoactivity Of Tachykinins In Isolated Rat Kidney: Functional Studies And In Vitro Receptor Autoradiography, Yuejin Chen

Electronic Theses and Dissertations

Although tachykinins have potent vascular actions, their effect on renal resistance blood vessels is currently unknown. The vasoactive properties of tachykinins and related analogs were assessed in isolated perfused rat kidney. At a basal perfusion pressure (PP) of 75 $\pm$ 6 mm Hg (n = 5), bolus injections of substance P (SP) had no significant vasoactive effect. Following a sustained increase in baseline PP (134 $\pm$ 10 mm Hg) produced by phenylephrine (1 $\mu$M), SP evoked a dose-dependent increase in PP. The largest dose of SP increased PP by 60 $\pm$ 5 mm Hg. The vasoconstrictor response to SP was …

Denervation Supersensitivity Of The Rat Vas Deferens: A Role For Protein Kinase C, Sonny T. Abraham

Electronic Theses and Dissertations

A role for protein kinase C (PKC) in the denervation-induced supersensitivity of the rat vas deferens was investigated. Chronic, surgical denervation of the rat vas deferens (up to 8 days) resulted in tissues that produced enhanced contractile responses to norepinephrine (NE) in isolated organ baths. Single challenges of NE (10 $\mu$M) produced 0.6 $\pm$ 0.1 g of maximal tension in the control vas whereas in the paired, denervated tissue 2.2 $\pm$ 0.3 g of tension was recorded (n = 6). Cumulative concentration-effect curves to NE produced in the denervated vas deferens were shifted 18-fold to the left of the control …