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Full-Text Articles in Forest Sciences

Utilization Of Sucrose During Cocultivation Positively Affects Agrobacterium-Mediatedtransformation Efficiency In Sugar Beet (Beta Vulgaris L.), Songül Gürel, Muhammet Çağri Oğuz, Ferzat Turan, Kemal Kazan, Canan Yüksel Özmen, Ekrem Gürel, Ali̇ Ergül Jan 2019

Utilization Of Sucrose During Cocultivation Positively Affects Agrobacterium-Mediatedtransformation Efficiency In Sugar Beet (Beta Vulgaris L.), Songül Gürel, Muhammet Çağri Oğuz, Ferzat Turan, Kemal Kazan, Canan Yüksel Özmen, Ekrem Gürel, Ali̇ Ergül

Turkish Journal of Agriculture and Forestry

Sugar beet (Beta vulgaris L.) is one of the most important industrial crops throughout world. With the availability of suitable genetic transformation technologies, the yield, quality, and stress tolerance of sugar beet could be improved significantly. However, low transformation efficiencies seriously limit the application of molecular technologies to the genetic improvement of sugar beet. With the aim of improving gene transfer techniques for sugar beet, the effect of different sucrose concentrations during cocultivation on the initial Agrobacterium-mediated transformation efficiencies in sugar beet was tested. To develop an efficient experimental system through which the effect of sucrose could be tested, first, …


Deciphering Global Dna Variations And Embryo Sac Fertility In Autotetraploid Rice Line, Fozia Ghouri, Jiani Zhu, Hang Yu, Jinwen Wu, Faheem Shahzad Baloch, Xiangdong Liu, Muhammad Qasim Shahid Jan 2019

Deciphering Global Dna Variations And Embryo Sac Fertility In Autotetraploid Rice Line, Fozia Ghouri, Jiani Zhu, Hang Yu, Jinwen Wu, Faheem Shahzad Baloch, Xiangdong Liu, Muhammad Qasim Shahid

Turkish Journal of Agriculture and Forestry

Autotetraploid rice is a new germplasm resource obtained by doubling chromosomes through colchicine treatment. There have been many studies on the reproductive characteristics of autotetraploid rice, but little is known about global DNA variations and reasons for low embryo sac fertility in autotetraploid rice. Therefore, an autotetraploid rice line (T446) was used for resequencing and embryo sac fertility was observed. Whole-genome resequencing data revealed 87,229 SNPs and 11,022 InDels in the genome of T446 versus E246 (diploid rice), which had an average of 23.37 SNPs and 2.95 InDels per 100 kb. A total of 17,375 and 17,171 structural variations and …