Biology Commons^™

In Vivo Structures Of The Helicobacter Pylori Cag Type Iv Secretion System, Yi-Wei Chang, Carrie L. Shaffer, Lee A. Rettberg, Debnath Ghosal, Grant J. Jensen

Veterinary Science Faculty Publications

The type IV secretion system (T4SS) is a versatile nanomachine that translocates diverse effector molecules between microbes and into eukaryotic cells. Here, using electron cryotomography, we reveal the molecular architecture of the Helicobacter pylori cag T4SS. Although most components are unique to H. pylori, the cag T4SS exhibits remarkable architectural similarity to other T4SSs. Our images revealed that, when H. pylori encounters host cells, the bacterium elaborates membranous tubes perforated by lateral ports. Sub-tomogram averaging of the cag T4SS machinery revealed periplasmic densities associated with the outer membrane, a central stalk, and peripheral wing-like densities. Additionally, we resolved pilus-like …

Zika Virus Can Strongly Infect And Disrupt Secondary Organizers In The Ventricular Zone Of The Embryonic Chicken Brain, Ankita Thawani, Devika Sirohi, Richard J. Kuhn, Donna Fekete

Department of Biological Sciences Faculty Publications

Zika virus (ZIKV) is associated with severe neurodeve- lopmental impairments in human fetuses, including microencephaly. Previous reports examining neural progenitor tropism of ZIKV in organoid and animal models did not address whether the virus infects all neural progenitors uniformly. To explore this, ZIKV was injected into the neural tube of 2-day-old chicken embryos, resulting in nonuniform periventricular infec- tion 3 days later. Recurrent foci of intense infection were present at specific signaling centers that influ- ence neuroepithelial patterning at a distance through secretion of morphogens. ZIKV infection reduced transcript levels for 3 morphogens, SHH, BMP7, and FGF8 expressed at the …

Inhibition Of Insulin‐Like Growth Factor 1 Receptor Enhances The Efficacy Of Sorafenib In Inhibiting Hepatocellular Carcinoma Cell Growth And Survival, Fang Wang, Thomas Bank, George Malnassy, Maribel Arteaga, Na Shang, Annika Dalheim, Xianzhong Ding, Scott J. Cotler, Mitchell F. Denning, Michael I. Nishimura, Peter Breslin, Wei Qiu

Biology: Faculty Publications and Other Works

Hepatocellular carcinoma (HCC) is the fifth most common primary cancer and second largest cause of cancer‐related death worldwide. The first‐line oral chemotherapeutic agent sorafenib only increases survival in patients with advanced HCC by less than 3 months. Most patients with advanced HCC have shown limited response rates and survival benefits with sorafenib. Although sorafenib is an inhibitor of multiple kinases, including serine/threonine‐protein kinase c‐Raf, serine/threonine‐protein kinase B‐Raf, vascular endothelial growth factor receptor (VEGFR)‐1, VEGFR‐2, VEGFR‐3, and platelet‐derived growth factor receptor β, HCC cells are able to escape from sorafenib treatment using other pathways that the drug insufficiently inhibits. The aim …

Potential Celecoxib Analogue Precursors Derived Via Aldol Condensation, Danny J, Stanley, Faith M. Peters, Mark T. Blankenbuehler

Celebration of Student Scholarship Poster Sessions Archive

No abstract provided.

Novel Regulator Of Vasopressin Secretion: Phoenixin, Silvia Gasparini, Lauren M. Stein, Spencer P. Loewen, Christopher J. Haddock, Jasmine Soo, Alastair V. Ferguson, Grant R. Kolar, Gina L.C. Yosten, Willis K. Samson

Biological Sciences Faculty Research & Creative Works

The newly described hypothalamic peptide, phoenixin, is produced in the hypothalamus and adenohypophysis, where it acts to control reproductive hormone secretion. Both phoenixin and its receptor GPR173 are expressed in the hypothalamic supraoptic (SON) and paraventricular (PVN) nuclei, suggesting additional, nonreproductive effects of the peptide to control vasopressin (AVP) or oxytocin (OT) secretion. Hypothalamo-neurohypophysial explants released AVP but not OT in response to phoenixin. Intracerebroventricular administration of phoenixin into conscious, unrestrained male and female rats significantly increased circulating AVP, but not OT, levels in plasma, and it increased immediate early gene expression in the supraoptic nuclei of male rats. Bath …

Gymnosperms On The Edge, Félix Forest, Justin Moat, Elisabeth Baloch, Neil A. Brummitt, Steve P. Bachman, Steffi Ickert-Bond, Peter M. Hollingsworth, Aaron Liston, Damon P. Little, Sarah Mathews, Hardeep Rai, Catarina Rydin, Dennis W. Stevenson, Philip Thomas, Sven Buerki

Wildland Resources Faculty Publications

Driven by limited resources and a sense of urgency, the prioritization of species for conservation has been a persistent concern in conservation science. Gymnosperms (comprising ginkgo, conifers, cycads, and gnetophytes) are one of the most threatened groups of living organisms, with 40% of the species at high risk of extinction, about twice as many as the most recent estimates for all plants (i.e. 21.4%). This high proportion of species facing extinction highlights the urgent action required to secure their future through an objective prioritization approach. The Evolutionary Distinct and Globally Endangered (EDGE) method rapidly ranks species based on their evolutionary …

Characterizing Putative Algal Bicarbonate Transporters In S. Cerevisiae & E. Coli Systems Using Transgenic Vectors, James J. Mickler

Honors Capstones

No abstract provided.

Gymnosperms On The Edge, Félix Forest, Justin Moat, Elisabeth Baloch, Neil A. Brummitt, Steve P. Bachman, Steffi Ickert-Bond, Peter M. Hollingsworth, Aaron Liston, Damon P. Little, Sarah Mathews, Hardeep Rai, Catarina Rydin, Dennis W. Stevenson, Philip Thomas, Sven Buerki

Biology Faculty Publications and Presentations

Driven by limited resources and a sense of urgency, the prioritization of species for conservation has been a persistent concern in conservation science. Gymnosperms (comprising ginkgo, conifers, cycads, and gnetophytes) are one of the most threatened groups of living organisms, with 40% of the species at high risk of extinction, about twice as many as the most recent estimates for all plants (i.e. 21.4%). This high proportion of species facing extinction highlights the urgent action required to secure their future through an objective prioritization approach. The Evolutionary Distinct and Globally Endangered (EDGE) method rapidly ranks species based on their evolutionary …

Prevalence Of Artemisinin Resistance Associated Single Nucleotide Polymorphisms In Kenyan Isolates, Elizabeth M. Glenn

Biology ETDs

Malaria has had an unspeakable toll on human economy and wellbeing. Every year, there are approximately 212 million malaria infections caused by Plasmodium falciparum leading to 429,000 deaths, the vast majority of which occur in sub-Saharan Africa where the parasite is endemic. Decades of widespread use, and probable misuse, of antimalarial drugs has created extraordinary selective pressure on the parasite and made many formerly effective drugs ineffective. Currently, artemisinin and artemisinin derivatives are the most effective treatment available but resistance to dihydroartemisinin (DHA), the active ingredient, is emerging. Continued monitoring for genetic mutations linked with resistance is vital to global …

Persistent Borrelia Infection In Patients With Ongoing Symptoms Of Lyme Disease, Marianne J. Middelveen, Eva Sapi, Jennie Burke, Katherine Filush, Agustin Franco, Melissa Fesler, Raphael B. Stricker

Biology and Environmental Science Faculty Publications

Introduction: Lyme disease is a tickborne illness that generates controversy among medical providers and researchers. One of the key topics of debate is the existence of persistent infection with the Lyme spirochete, Borrelia burgdorferi, in patients who have been treated with recommended doses of antibiotics yet remain symptomatic. Persistent spirochetal infection despite antibiotic therapy has recently been demonstrated in non-human primates. We present evidence of persistent Borrelia infection despite antibiotic therapy in patients with ongoing Lyme disease symptoms. Methods: In this pilot study, culture of body fluids and tissues was performed in a randomly selected group of 12 patients with …

Seasonal Shifts In C3 And C4 Resource Use By A Small Mammal Community Under Changing Precipitation Regimes, Alaina Dianne Pershall

Biology ETDs

In light of climate change and projections of increasing temperatures and aridity in the North American southwest, it is essential to understand how consumer populations will respond to changes in the resource landscape. Rainfall varies in timing and intensity and therefore the timing, proportion, phenology, and abundance of C₃ and C₄ plant resources vary seasonally and annually. Here we examine rodent resource use in the Chihuahuan desert and focus on two distinct precipitation pulses in this system, where spring C₃ plants increase production in response to winter rains and C₄ plants respond to summer monsoons. We …

Bioaccumulation Of Zinc In Bermuda Grass (Cynodon Dactylon), Drew Fisher, H. Marie Surratt, Louise Gebauer, Andrew Palaski

Featured Research

Phytoremediation is the plant based process to remove toxins such as heavy metals from the soil. In this experiment, we hypothesized Bermuda grass (Cyndon dactylon) will absorb a detectable level of zinc, a nontoxic metal, from the soil. We grew two separate groups of grass: the control and the experimental, which was watered with zinc acetate. Neither of the groups produced enough yield within the weeks given to the experiment, but if it went according to plan, we would have used flame atomic absorption spectroscopy to evaluate the relative concentrations of zinc. Significant uptake of zinc could reveal a possible …

A Comparison Of Electron Transport Rate, Photosynthetically Active Radiation, Light-Adapted Fluorescence, And Dark-Adapted Fluorescence Between Stipa Pulchra And Pennisetium Setaceum, Sydney Adams, Brandon Chuck, Agatha Heng, Amelia Vento, Stephen D. Davis

Featured Research

The purpose of this investigation was to provide an explanation into how the invasive species Pennisetium setaceum, Fountain Grass, is able to outcompete the native California grassland species Stipa pulchra, Purple Needle Grass. We used a light adapted and a dark adapted fluorometer to measure the photosynthetic radiation (PAR), leaf temperature, alpha (α), light adapted fluorescence (Fv’/Fm’), dark adapted fluorescence (Fv/Fm), and the electron transport rate (ETR) on young Fountain Grass and Purple Needlegrass. After collecting and analyzing the data, we concluded that the dark adapted fluorescence (Fv/Fm) was the only statistically significant measurement where the Fv/Fm of S. pulchra …

Genomic Analysis Of The Novel Mycobacteriophage Bromden, Kaitlyn Lein, Kimberly Paquette, Timbree Pederson, Matthew Switalski, Carter Smiley, Emma Oonk, Darby Baird, Brooke Van Wyk, Lilly Hemesath, Abigail Meder, Madelyn Orndorff, Megan Grimes

17th Annual Celebration of Undergraduate Research and Creative Performance (2018)

Twenty new mycobacteriophages were isolated from soil samples collected around the state of Michigan and other parts of the United States. All phages were capable of infecting Mycobacterium smegmatis and were isolated through either enrichment or direct plating at 32℃. A variety of plaque morphologies were produced based on size, shape, and clarity; both lytic and temperate phages are represented in this collection. The mycobacteriophage, Bromden, was chosen as one of two phages for complete genome sequencing and comparative genomic analyses. The predominant plaque produced by Bromden after 48 hours at 32℃ is 0.5 mm in diameter, circular, and clear. …

Using Δ13c, Δ15n, And Δ2h To Better Understand The Ecology Of Green Sea Turtles, Laura Pagès Barceló

Biology ETDs

Many green sea turtle populations are slowly recuperating from a recent severe decline due to anthropogenic factors including human consumption and mortality related to the fishing industry. Despite being charismatic animals that have been extensively studied, there is still a limited understanding of their feeding strategies and diet plasticity. This research explores the use of hydrogen isotopes in marine ecosystems to better understand green sea turtle ecology. This study is presented in two chapters: I first examined the trophic discrimination factor (D²H_NET) for hydrogen isotope (d²H) as a tool to correct hydrogen isotope data …

Observations On The Reproductive Biology Of The Darter Fish Etheostoma Kennicotti In Response To Gill Parasite Infections, Zeina Sleiman, Corinne Peacher, Joy Garcia

Research Horizons Day Posters

No abstract provided.

Cytotoxicity Of Plant Extracts On Mcf-7 And Hs578t Breast Cell Lines, Brianne Brazell

Research Horizons Day Posters

No abstract provided.

Testing Viability Of Oligonucleotide Primers On Microsatellite Markers Amplified By Polymerase Chain Reaction On Dermacentor Variabilis Samples From The Bulloch County Region Of Georgia, Usa, Danielle Souza

Honors College Theses

Dermacentor variabilis, more commonly known as the wood tick, or the American dog tick, can be found all over North America. D. variabilis is a common tick in Bulloch county, Georgia, USA, and little is known about the population structure of this population of the species. Microsatellite markers are able to locate sections of the genome and can aid in estimating relatedness among individuals in a population. Six different oligonucleotide primer pairs were published for D. variabilis, however, published and non-published works indicated that not all of those primer pairs were working equally well for PCR. This study is …

The Successful Exploitation Of Urban Environments By The Golden Silk Spider, Nephila Clavipes (Araneae, Nephilidae), Jake Ripp, Omar T. Eldakar, Andrew C. Gallup, Paul T. Arena

Biology Faculty Articles

Urbanization typically leads to habitat destruction producing negative effects for native species, but some species may exploit these settings. This concept was investigated in the golden silk spider (Nephila clavipes), a large, formidable spider that commonly inhabits forest edges as well as open spaces in urban environments throughout its vast geographic range. Here, we compared variation of N. clavipes success as measured by body size, web size and web positioning along an urban–rural gradient in southern Florida. From morphological measurements collected in the field, urban spiders had 60% longer legs and 35% longer bodies than both park and …

2018 Senior Research Symposium, Otterbein University Department Of Biology & Earth Science

Biology and Earth Science Student Scholarship

This is the schedule for the 2018 Senior Research Symposium and the abstracts from each presentation.

High Male Sexual Investment As A Driver Of Extinction In Fossil Ostracods, Maria João Fernandes Martins, T. Markham Puckett, Rowan Lockwood, John P. Swaddle, Gene Hunt

Faculty Publications

Sexual selection favours traits that confer advantages in the competition for mates. In many cases, such traits are costly to produce and maintain, because the costs help to enforce the honesty of these signals and cues. Some evolutionary models predict that sexual selection also produces costs at the population level, which could limit the ability of populations to adapt to changing conditions and thus increase the risk of extinction. Other models, however, suggest that sexual selection should increase rates of adaptation and enhance the removal of deleterious mutations, thus protecting populations against extinction. Resolving the conflict between these models is …

De Novo Assembly Of The Burying Beetle Nicrophorus Orbicollis (Coleoptera: Silphidae) Transcriptome Across Developmental Stages With Identification Of Key Immune Transcripts, Harim Won, Thomas T. Schulze, Emalie J. Clement, Gabrielle F. Watson, Sean M. Watson, Rosalie C. Warner, Elizabeth A. M. Ramler, Elias J. Witte, Mark A. Schoenbeck, Claudia M. Rauter, Paul H. Davis

Biology Faculty Publications

Burying beetles (Nicrophorus spp.) are among the relatively few insects that provide parental care while not belonging to the eusocial insects such as ants or bees. This behavior incurs energy costs as evidenced by immune deficits and shorter life-spans in reproducing beetles. In the absence of an assembled transcriptome, relatively little is known concerning the molecular biology of these beetles. This work details the assembly and analysis of the Nicrophorus orbicollis transcriptome at multiple developmental stages. RNA-Seq reads were obtained by next-generation sequencing and the transcriptome was assembled using the Trinity assembler. Validation of the assembly was performed by functional …

The Effects Of Soil Suffused With Cool-Fire Ash On The Germination And Early Growth Of Radish Seedlings, Ryan Dalforno, Braden Ritchey, Jay Ogle

Featured Research

In this study, the effects of cool fire ash in soil with varying amounts of water were observed in terms of the germination and early growth of garden radish (Raphanus raphanistrum subsp. sativus) seedlings. Measurements regarding plant growth, field capacity, and seedling leaf quantum yield were taken and compared between four (4) trios of pots separately containing radish seedlings planted in ashy and frequently-watered soil (A_w), ashy and infrequently-watered soil (A_d), normal unmodified garden soil that was watered frequently (N_w) and normal soil that was watered infrequently (N_d). While the …

A Comparison Of Fluorescence And Etr Between Malosma Laurina And Rhus Integrifolia, Talia Danze, Jennifer Ipe, Viridiana Hernandez-Lopez, Stephen D. Davis, Talia Cao

Featured Research

Malosma laurina and Rhus integrifolia are both native species to the Santa Monica Mountains and belong to the same family, Anacardiaceae. The two natives have lived alongside each other but in recent years M. laurina has been heavily affected by the prolonged drought. The Malosma laurina population in the Santa Monica Mountains has withstood wildfires and droughts, and has remained relatively stable and healthy up until recently. A recent Pepperdine graduate published her findings explaining the high levels of dieback in Malosma laurina and attributed it to the fungus, B. dothidea. We hypothesized that Rhus integrifolia would have higher fluorescence …

A Comparison Of Electron Transport Rate, Photosynthetically Active Radiation, Light-Adapted Fluorescence, And Dark-Adapted Fluorescence Between Stipa Pulchra And Pennisetium Setaceum, Sydney Adams, Brandon Chuck, Agatha Heng, Amelia Vento, Stephen D. Davis

Featured Research

The purpose of this investigation was to provide an explanation into how the invasive species Pennisetium setaceum, Fountain Grass, is able to outcompete the native California grassland species Stipa pulchra, Purple Needle Grass. We used a light adapted and a dark adapted fluorometer to measure the photosynthetic radiation (PAR), leaf temperature, alpha (α), light adapted fluorescence (Fv’/Fm’), dark adapted fluorescence (Fv/Fm), and the electron transport rate (ETR) on young Fountain Grass and Purple Needlegrass. After collecting and analyzing the data, we concluded that the dark adapted fluorescence (Fv/Fm) was the only statistically significant measurement where the Fv/Fm of …

A Comparison Of Electron Transport Rate, Photosynthetically Active Radiation, Light-Adapted Fluorescence, And Dark-Adapted Fluorescence Between Stipa Pulchra And Pennisetium Setaceum, Sydney Adams, Brandon Chuck, Agatha Heng, Amelia Vento, Stephen D. Davis

Featured Research

The purpose of this investigation was to provide an explanation into how the invasive species Pennisetium setaceum, Fountain Grass, is able to outcompete the native California grassland species Stipa pulchra, Purple Needle Grass. We used a light adapted and a dark adapted fluorometer to measure the photosynthetic radiation (PAR), leaf temperature, alpha (α), light adapted fluorescence (Fv’/Fm’), dark adapted fluorescence (Fv/Fm), and the electron transport rate (ETR) on young Fountain Grass and Purple Needlegrass. After collecting and analyzing the data, we concluded that the dark adapted fluorescence (Fv/Fm) was the only statistically significant measurement where the Fv/Fm of S. pulchra …

A Comparison Of Electron Transport Rate, Photosynthetically Active Radiation, Light-Adapted Fluorescence, And Dark-Adapted Fluorescence Between Stipa Pulchra And Pennisetium Setaceum, Sydney Adams, Brandon Chuck, Agatha Heng, Amelia Vento, Stephen D. Davis

Featured Research

The purpose of this investigation was to provide an explanation into how the invasive species Pennisetium setaceum, Fountain Grass, is able to outcompete the native California grassland species Stipa pulchra, Purple Needle Grass. We used a light adapted and a dark adapted fluorometer to measure the photosynthetic radiation (PAR), leaf temperature, alpha (α), light adapted fluorescence (Fv’/Fm’), dark adapted fluorescence (Fv/Fm), and the electron transport rate (ETR) on young Fountain Grass and Purple Needlegrass. After collecting and analyzing the data, we concluded that the dark adapted fluorescence (Fv/Fm) was the only statistically significant measurement where the Fv/Fm of S. pulchra …

Skeletal, Cardiac, And Respiratory Muscle Function And Histopathology In The P448lneo- Mouse Model Of Fkrp-Deficient Muscular Dystrophy., Qing Yu, Melissa Morales, Ning Li, Alexander G Fritz, Ren Ruobing, Anthony Blaeser, Ershia Francois, Qi-Long Lu, Kanneboyina Nagaraju, Christopher F Spurney

Genomics and Precision Medicine Faculty Publications

BACKGROUND: Fukutin-related protein (FKRP) mutations are the most common cause of dystroglycanopathies known to cause both limb girdle and congenital muscular dystrophy. The P448Lneo- mouse model has a knock-in mutation in the FKRP gene and develops skeletal, respiratory, and cardiac muscle disease.

METHODS: We studied the natural history of the P448Lneo- mouse model over 9 months and the effects of twice weekly treadmill running. Forelimb and hindlimb grip strength (Columbus Instruments) and overall activity (Omnitech Electronics) assessed skeletal muscle function. Echocardiography was performed using VisualSonics Vevo 770 (FujiFilm VisualSonics). Plethysmography was performed using whole body system (ADInstruments). Histological evaluations included …

Tgf-Β2 In Human Milk Research: Exploration Of A New Field Methodology And New Findings Of Biosimilar Tgf-Β2 In Non-Human Milk, Chlöe A. Sweetman

USF Tampa Graduate Theses and Dissertations

Objectives: There are three aims for this thesis: the first is to develop a field and laboratory protocol for the storage and analysis of transforming growth factor–beta 2 (TGF-β2) in human breastmilk; second, to validate this protocol and the immunoassay used to assess this new method; and lastly, to explore the ramifications of biosimilar TGF-β2 across multiple milks on human health, growth, and immunity through the review of laboratory findings and previous literature.

Rational: Little anthropological research has been done on TGF-β2 in human milk. Anthropology as a discipline is well positioned to provide insight into TGF-β2, combining biocultural, evolutionary, …

The Effects Of The Omega-3 Fatty Acid Decosahexaenoic Acid On Intracellular Myo-Inositol Levels And The Ino1 Gene Expression In Saccharomyces Cerevisiae, Taejun Ok

Honors Theses

Bipolar disorder is a mental disorder portrayed with repeated episodes of mania and depression. Its current treatments often entail inefficacy and negative side effects. From previous clinical research, omega-3-fatty acids were suggested to be effective in the amelioration of bipolar disorder. Such an effect is hypothetically attributed to their ability to alter the level of myo-inositol, a molecule that valproate and lithium are known to deplete out. Thus, the goal of this study is to determine the effects of the omega-3 decosahexaenoic acid (DHA) on myo-inositol levels and the expression of the myo-inositol synthase gene INO1. Yeast cells were cultured …