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Full-Text Articles in Biodiversity

Demography Of Whooping Cranes In The Eastern Migratory Population, Sarah J. Converse, Richard P. Urbanek Jan 2010

Demography Of Whooping Cranes In The Eastern Migratory Population, Sarah J. Converse, Richard P. Urbanek

Proceedings of the North American Crane Workshop

The ultimate success of the whooping crane (Grus americana) reintroduction to eastern North America rests on adequate demographic performance of the population. We are undertaking a population viability analysis (PVA) of the eastern migratory population in order to evaluate progress toward the fundamental population objective, to better understand the critical demographic thresholds that must be met to fulfill this objective, and, most importantly, to support management decision-making. The initial phase in the PVA development process involves estimation of demographic parameters to be used in later population modeling phases. Multi-state models provide an appropriate analytic framework for estimation, wherein …


Current Status Of Lesser Sandhill Cranes In Yakutia, Inga Bysykatova, Sergey Sleptsov, Nikolay Vasiliev Jan 2010

Current Status Of Lesser Sandhill Cranes In Yakutia, Inga Bysykatova, Sergey Sleptsov, Nikolay Vasiliev

Proceedings of the North American Crane Workshop

In Yakutia, the sandhill crane (Grus canadensis canadensis) was considered a common bird in 1957 on the Primorie tundra from the Kolyma River to the Alazeya River. In 1980 the area of the species’ supposed breeding grounds within the Kolyma- Indigirka interfluve comprised 34,600 km2. In 1984-85, the breeding grounds extended west to the Sundrun River, with the total area reaching 49,400 km2. At present, the westernmost sandhill crane range is on the tundra along the lower reaches of the Berelekh River. This region joins the higher density Siberian crane (G. leucogeranus) range, so that the …


Summary Of Sandhill Crane Sport Harvest In Canada 1975–2006, Adrianna C. Araya, Kammie L. Kruse, Keith D. Warner Jan 2010

Summary Of Sandhill Crane Sport Harvest In Canada 1975–2006, Adrianna C. Araya, Kammie L. Kruse, Keith D. Warner

Proceedings of the North American Crane Workshop

Knowledge of harvest in all areas where the mid-continent population (MCP) of sandhill cranes (Grus canadensis) occurs is critical to managing the population in a sustainable manner. The harvest of MCP in the U.S. has been well documented; however, the harvest in Canada has received less attention. The Canadian Wildlife Service initiated a National Harvest Survey program in 1967, but all sampling variables were not directly comparable until 1975. In this paper, we summarize crane harvest in Canada during the 1975-2006 hunting seasons for Saskatchewan and Manitoba, the 2 provinces with significant sport hunting harvest of sandhill cranes. …


Demoiselle Cranes On Agricultural Lands In The Ukraine, Yuriy Andryushchenko Jan 2010

Demoiselle Cranes On Agricultural Lands In The Ukraine, Yuriy Andryushchenko

Proceedings of the North American Crane Workshop

In Eurasia, the western range of the demoiselle crane (Anthropoides virgo) includes the southeast of Ukraine. Here the species nests, forms flying and pre-migrating concentrations, and participates in migration. The number of demoiselle cranes in April-June is about 600-700 individuals (200-250 nesting pairs), and toward the end of the year the population can reach 900-1000 individuals. Of cranes observed during 2000-2008 in the Crimea, 42.3% of pairs (n = 151) nested on agricultural fields: 21.5% on virgin land, 16.1% on cropland, and 3.4% on fallow lands. Principal characteristics of the demoiselle crane nesting locations were wavy relief, scarce herbage, …


Possible Competition Between Waterfowl And Sandhill Cranes At Hiwassee Wildlife Refuge, Tennessee, David A. Aborn Jan 2010

Possible Competition Between Waterfowl And Sandhill Cranes At Hiwassee Wildlife Refuge, Tennessee, David A. Aborn

Proceedings of the North American Crane Workshop

As a result of crop planting for waterfowl, numbers of eastern greater sandhill cranes (Grus canadensis tabida) staging and overwintering at the Hiwassee Wildlife Refuge in eastern Tennessee have sharply increased over the last 30-40 years. Peak numbers of wintering cranes have reached 14,000, and this large increase in crane numbers raises the possibility that they may be competing with waterfowl for food and space. I examined broad-scale changes in waterfowl numbers using Christmas Bird Count data, as well as small-scale changes using observations of waterfowl numbers and locations in relation to cranes on individual days. Preliminary results …


Behavior And Habitat Use Of Greater Sandhill Cranes Wintering In East Tennessee, David A. Aborn Jan 2010

Behavior And Habitat Use Of Greater Sandhill Cranes Wintering In East Tennessee, David A. Aborn

Proceedings of the North American Crane Workshop

An increase of eastern greater sandhill cranes (Grus canadensis tabida) stopping over and wintering at the Hiwassee Wildlife Refuge in east Tennessee has led to overcrowding at the refuge, resulting in crane use of private property. Proper management is needed to prevent the cranes from becoming a nuisance, increase the suitability of the refuge for waterfowl, and reduce the likelihood of a disease outbreak. Observations of cranes revealed that cranes spent 83% of their time foraging, alert, or preening. Sandhill cranes foraged primarily in agricultural and grassy fields, whereas mudflats and shallow water were used for preening and …


First Breeding Records And Historical Status Of Sandhill Cranes In New England, Scott M. Melvin Jan 2010

First Breeding Records And Historical Status Of Sandhill Cranes In New England, Scott M. Melvin

Proceedings of the North American Crane Workshop

Sandhill cranes (Grus canadensis) nested at 4 sites in south-central Maine between 2000 and 2008 and at single sites in western Massachusetts and west-central Vermont in 2007 and 2008, continuing their eastward range expansion. Of 13 nests observed, 5 were in a lacustrine marsh, 2 were in a riverine marsh, and 2 were in beaver-impounded palustrine marshes, all dominated by cattail (Typha spp.); 2 were in lacustrine fen habitat dominated by sedges (Carex spp.), sphagnum, and leatherleaf (Chamaedaphne calyculata); 1 was in a lacustrine fen dominated by slender sedge (Carex lasiocarpa …


Mechanisms Of Habitat Selection Of Reintroduced Whooping Cranes On Their Breeding Range, Kelly J. Maguire, Stanley A. Temple Jan 2010

Mechanisms Of Habitat Selection Of Reintroduced Whooping Cranes On Their Breeding Range, Kelly J. Maguire, Stanley A. Temple

Proceedings of the North American Crane Workshop

We examined several mechanisms that influenced the habitat selection of reintroduced whooping cranes (Grus americana) on their breeding range in the midwestern United States. Visual observations on 56 whooping cranes from 2001 to 2006 provided accurate locations, habitat descriptions, and bird associations. Location information on each bird was mapped to create home range and to describe the habitat. We found evidence that habitat selection in these cranes resulted from multiple mechanisms, including habitat imprinting, philopatry, site tenacity, intra-specific interactions, and environmental stochasticity. The initial home ranges of all cranes contained habitat similar to that in which they were …


Sandhill Crane Migration Chronology And Behavior In Northwestern Texas, Carroll D. Littlefield Jan 2010

Sandhill Crane Migration Chronology And Behavior In Northwestern Texas, Carroll D. Littlefield

Proceedings of the North American Crane Workshop

Migrant lesser sandhill cranes (Grus canadensis canadensis) were monitored during 1990-2000 in northwestern Texas as flocks were arriving in autumn and departing in spring; cranes were counted as they passed over an observation point 23 km north of Sudan, Lamb County, Texas. Mean flock size was 34.5 (SD ± 32.6) in autumn and 58.2 (SD ± 45.0) in spring. Most autumn migrants (69.6%) passed in the afternoon, whereas in spring 94% migrated in the morning (1000-1200 hr). Peak period of autumn passage was in October (65%), and in spring all observations were in February-March with the peak usually …


Population Status And Geographic Distribution Of Greater Sandhill Cranes In The Mid-Continent Population, Gary L. Krapu, David A. Brandt Jan 2010

Population Status And Geographic Distribution Of Greater Sandhill Cranes In The Mid-Continent Population, Gary L. Krapu, David A. Brandt

Proceedings of the North American Crane Workshop

Number and geographic distribution of greater sandhill cranes (Grus canadensis tabida) in the mid-continent population (MCP) of sandhill cranes were evaluated. G. c. tabida comprised 7 of 133 (5.3%) individuals of 3 subspecies which projects to 31,579 ± 11,661 (SE) individuals in an estimated spring MCP of 600,000 cranes. From a platform transmitting terminal (PTT)-marked sample representative of the geographic distribution of G. c. tabida, 10 of 13 (77%) settled during the breeding season in east-central Canada/Minnesota, including 4 in northwestern Minnesota, 4 in Manitoba (2 at sites near the Minnesota border), and 2 in Ontario. Three …


Migration Patterns And Movements Of Sandhill Cranes Wintering In Central And Southwestern Louisiana, Sammy L. King, Aaron R. Pierce, Kent R. Hersey, Nicholas Winstead Jan 2010

Migration Patterns And Movements Of Sandhill Cranes Wintering In Central And Southwestern Louisiana, Sammy L. King, Aaron R. Pierce, Kent R. Hersey, Nicholas Winstead

Proceedings of the North American Crane Workshop

In this study we trapped wintering sandhill cranes (Grus canadensis) in Louisiana and fitted them with satellite transmitters to determine their migration routes. Four of the 6 sandhill cranes with validated locations and a terminus point used the Central Flyway for spring migration; 2 of these 4 (the only 2 for which we have data) also used the Central Flyway for fall migration. Two of the 6 birds used the Mississippi Flyway for spring migration. The results of this study suggest that reintroduced whooping cranes (G. americana) that intermix and migrate with sandhill cranes that winter …


Countability Of Sandhill Cranes In Aerial Surveys, Douglas H. Johnson, John W. Solberg, Courtney L. Amundson Jan 2010

Countability Of Sandhill Cranes In Aerial Surveys, Douglas H. Johnson, John W. Solberg, Courtney L. Amundson

Proceedings of the North American Crane Workshop

Aerial surveys are used to monitor populations of many wildlife species, including sandhill cranes (Grus canadensis). In addition to the usual problems of detectability (involving both availability and perceptibility), aerial surveys of concentrated animals are subject to countability issues; from a rapidly moving aircraft, observers cannot count or accurately estimate the number of animals in a large group. Calibration is sometimes performed in an effort to adjust aerial counts for incomplete detectability and countability by calculating the ratio of animals actually in a group to the number in the group estimated from the aircraft. Here we explore alternative, …


Aerial Survey Techniques For Breeding Whooping Cranes, Brian W. Johns Jan 2010

Aerial Survey Techniques For Breeding Whooping Cranes, Brian W. Johns

Proceedings of the North American Crane Workshop

Since the discovery of nesting whooping cranes in Wood Buffalo National Park, the Canadian Wildlife Service has conducted aerial surveys to monitor the population. Aerial survey techniques have varied over the years; however, they have generally followed the techniques used by the author since 1991. The technique involves flying a combination of circular flights and transects over known nesting territories and similar looking marshes likely to contain breeding whooping cranes. These aerial surveys account for nearly 100% of the breeding whooping cranes each year.


Whooping Crane Migrational Habitat Use Of The Platte River, Nebraska, 2001–2006, Shay Howlin, Clayton Derby, Dale Strickland Jan 2010

Whooping Crane Migrational Habitat Use Of The Platte River, Nebraska, 2001–2006, Shay Howlin, Clayton Derby, Dale Strickland

Proceedings of the North American Crane Workshop

The Platte River Endangered Species Partnership monitored whooping crane (Grus americana) habitat use along the Platte River between Chapman and Lexington, Nebraska during 11 migration seasons from 2001 to 2006. Daily aerial surveys took place in the morning from 21 March to 29 April in the spring and from 9 October to 10 November in the fall. Decoy detection trials were conducted during each of the 11 survey seasons to calculate actual sample inclusion probabilities for crane groups detected during monitoring flights. The detectability model found significant differences in detectability among strata (upland or channel), contractor, and altitude …


Mississippi Sandhill Crane Conservation Update 2006–2008, Scott G. Hereford, Lauren E. Billodeaux Jan 2010

Mississippi Sandhill Crane Conservation Update 2006–2008, Scott G. Hereford, Lauren E. Billodeaux

Proceedings of the North American Crane Workshop

The Mississippi sandhill crane (MSC, Grus canadensis pulla) is an endangered non-migratory subspecies found in the wild only on and near the Mississippi Sandhill Crane National Wildlife Refuge (MSCNWR) in Jackson County in southeastern Mississippi (Gee and Hereford 1995). The MSC is adapted to the wet pine savannas and open pinelands of the Southeastern Outer Gulf Coastal Plain. By the 1970s, their numbers had decreased to 30-40 individuals including only 5-6 nesting pairs. The firemaintained savannas, once a dominant feature of the landscape, were converted to pine plantations and human development or had degraded to overgrown pine scrub. The …


Development Of The Whooping Crane Tracking Project Geographic Information System, Martha Tacha, Andy Bishop, Justin Brei Jan 2010

Development Of The Whooping Crane Tracking Project Geographic Information System, Martha Tacha, Andy Bishop, Justin Brei

Proceedings of the North American Crane Workshop

The highest losses in the Aransas-Wood Buffalo whooping crane population occur during migration. Conservation and recovery of the endangered whooping crane requires understanding of migration patterns to identify important stopover areas and potential sources of mortality or disturbance. We converted the Cooperative Whooping Crane Tracking Project database, containing more than 3 decades of data on whooping crane sightings, to a geographic information system (GIS) to allow coarse scale spatial analyses of whooping crane migration patterns in the United States portion of the Central Flyway. At this writing, the geodatabase contains point data for 1,981 confirmed whooping crane sightings through the …


A Retrospective Serological Survey For Infectious Bursal Disease Virus In Free-Ranging Sandhill Cranes In South-Central Wisconsin, Richard R. Sim, Barry K. Hartup Jan 2010

A Retrospective Serological Survey For Infectious Bursal Disease Virus In Free-Ranging Sandhill Cranes In South-Central Wisconsin, Richard R. Sim, Barry K. Hartup

Proceedings of the North American Crane Workshop

A retrospective serosurvey of resident sandhill cranes (Grus candensis pratensis) in Florida suggested Infectious Bursal Disease Virus (IBDV, family Birnaviridae) may have been enzootic there as early as 1992, with 13-63% seroprevalence among age classes and wide geographic distribution of seropositive cranes. The purpose of our study was to describe the prevalence of antibodies to IBDV serotype 2 in a local population of greater sandhill cranes (G. c. tabida) near Briggsville, Wisconsin, that overlapped with resident Florida birds in winter during the same time period. Blood samples were collected June through October between 1996 and 1999 …


Summary Of Sandhill Crane Hunting Seasons In Kansas 1993–2007, David E. Sharp, Helen M. Hands, James A. Dubovsky, John E. Cornely Jan 2010

Summary Of Sandhill Crane Hunting Seasons In Kansas 1993–2007, David E. Sharp, Helen M. Hands, James A. Dubovsky, John E. Cornely

Proceedings of the North American Crane Workshop

The mid-continent population of sandhill cranes (Grus canadensis) is the largest population of cranes in North America. Hunting seasons for sandhill cranes were closed in 1916 and gradually resumed in Mexico (1940), the United States (1961), and Canada (1964). As knowledge of the biology of cranes and experience with hunting seasons increased, areas in which hunting was permitted expanded, and by 1992 all but 2 states (Nebraska and Kansas) in the U.S. portion of the Central Flyway conducted sport hunting seasons for this population. In 1993 Kansas resumed hunting and initial seasons were limited to specified geographic areas of …


Proceedings Of The Eleventh North American Crane Workshop: Preface And Contents Jan 2010

Proceedings Of The Eleventh North American Crane Workshop: Preface And Contents

Proceedings of the North American Crane Workshop

The North American Crane Working Group (NACWG) is an organization of professional biologists, aviculturists, land managers, and other interested individuals dedicated to the conservation of cranes and their habitats in North America. Our group meets approximately every 3 years to exchange new information on research and conservation of sandhill and whooping cranes and occasionally reports from abroad. Our meeting in Wisconsin Dells, Wisconsin, 23-27 September 2008, was no exception. The workshop was ably organized by Tom Hoffman, the scientific program was well-constructed by Jane Austin, and George Archibald and Richard Urbanek helped to bring together a memorable field day in …


Wintering Sandhill Crane Distribution And Habitat Use Patterns At Bosque Del Apache National Wildlife Refuge, New Mexico, Tandi L. Perkins, Leigh H. Fredrickson Jan 2010

Wintering Sandhill Crane Distribution And Habitat Use Patterns At Bosque Del Apache National Wildlife Refuge, New Mexico, Tandi L. Perkins, Leigh H. Fredrickson

Proceedings of the North American Crane Workshop

The single most important factor regulating sandhill crane (Grus canadensis) populations is their ability to carry out annual life cycle events while responding to changing habitat availability and distribution across local, regional, and continental landscapes. Wetland and cropland resource availability and distribution across the landscape have become increasingly unpredictable. Recent changes in farming practices, urbanization, and prevailing drought conditions in New Mexico have transformed and reduced dynamic, heterogeneous landscapes into scattered fragments of the original setting. Concerns are increasing for the cumulative influence of these often irreversible actions in providing the type of resources needed by cranes at …


Prolonged Intensive Dominance Behavior Between Gray Wolves, Canis Lupus, L. David Mech, H. Dean Cluff Jan 2010

Prolonged Intensive Dominance Behavior Between Gray Wolves, Canis Lupus, L. David Mech, H. Dean Cluff

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Dominance is one of the most pervasive and important behaviors among wolves in a pack, yet its significance in free-ranging packs has been little studied. Insights into a behavior can often be gained by examining unusual examples of it. In the High Arctic near Eureka, Nunavut, Canada, we videotaped and described an unusually prolonged and intensive behavioral bout between an adult male Gray Wolf (Canis lupus) and a male member of his pack, thought to be a maturing son.With tail raised, the adult approached a male pack mate about 50 m from us and pinned and straddled this …


Biodiversity Assessment Of The Fishes Of Saba Bank Atoll, Netherlands Antilles, Jeffrey T. Williams, Kent E. Carpenter, James L. Van Tassell, Paul Hoetjes, Wes Toller, Peter Etnoyer, Michael Smith Jan 2010

Biodiversity Assessment Of The Fishes Of Saba Bank Atoll, Netherlands Antilles, Jeffrey T. Williams, Kent E. Carpenter, James L. Van Tassell, Paul Hoetjes, Wes Toller, Peter Etnoyer, Michael Smith

Biological Sciences Faculty Publications

Biodiversity surveys were conducted on Saba Bank, Netherlands Antilles, to assess ichthyofaunal richness and to compare with published surveys of other Caribbean localities. The primary objective was to estimate the total species richness of the Saba Bank ichthyofauna. A variety of sampling techniques was utilized to survey the fish species of both the visually accessible megafauna and the camouflaged and small-sized species comprising the cryptic ichthyofauna. Based on results presented herein, the number of species known on Saba Bank is increased from 42 previously known species to 270 species. Expected species-accumulation curves demonstrate that the current estimate of species richness …


A Taxonomic Revision Of Orthotrichaceae (Musci) With Contributions To The Biosystematics Of Macromitrium Brid. In Malaysia., Yong Kien Thai Jan 2010

A Taxonomic Revision Of Orthotrichaceae (Musci) With Contributions To The Biosystematics Of Macromitrium Brid. In Malaysia., Yong Kien Thai

Student Works (2010-2019)

Macromitrium (Orthotrichaceae) is a large genus variously divided into infrageneric groups by different authors, resulting in a complex classification which has not included many Malaysian species. This study reviews the taxonomy of Malaysian taxa and extent of morphological variation in relation to the type group. A molecular phylogenetic study was carried out to determine if Malaysian Macromitrium species represented a homogeneous taxonomic grouping. A cladistic analysis of morphological characteristics was attempted for identifying well-defined groups and their key character states. Molecular studies included Maximum Parsimony and Bayesian Inference analyses of two chloroplast DNA regions (rps4 gene and trnL-trnF interspecific region) …


Taxonomy And Biogeography Of Sundaland Gardenia (Rubiaceae)., Low Yee Wen Jan 2010

Taxonomy And Biogeography Of Sundaland Gardenia (Rubiaceae)., Low Yee Wen

Student Works (2010-2019)

In Southeast Asia, the genus Gardenia J.Ellis (Rubiaccae) is poorly understood and documented due to a lack of country revisions, contentious species delimitations and accumulation of poorly identified material in herbaria. This study reviewed the taxonomic history and position of the genus, and revised the taxonomy of Sundaland species (including those of Sumatra. the Malay Peninsula, Java_ Borneo, Palawan and adjacent islands). It includes a survey and cladistic study of morphological characteristics, and phylogenetic ana lyses using two chloroplast regions (atpB-rbcL and trnT-F) and the nuclear ITS region of representative species from Sundaland and adjoining regions. Twelve species are native …


Butterfly Response To Floral Resources At A Heterogeneous Prairie Biofuel Production Site, Benjamin J. Hoksch, Mark C. Myers Jan 2010

Butterfly Response To Floral Resources At A Heterogeneous Prairie Biofuel Production Site, Benjamin J. Hoksch, Mark C. Myers

Undergraduate Student Work

In the Midwestern USA, current biofuel production systems rely on high input monoculture crops that do little to support biodiversity or protect soil and water resources. The University of Northern Iowa’s Tallgrass Prairie Center is investigating the feasibility of cultivating and harvesting diverse mixes of native prairie vegetation as a sustainable biofuel. In 2009, we established 48 research plots seeded with one of four treatments: 1) switchgrass monoculture, 2) warm-season grass mix (5 species), 3) biomass mix (16 species of grasses and forbs), or 4) prairie mix (32 species of grasses and forbs). We hypothesized that more diverse plant communities …


Increased Weed Diversity, Density And Above-Ground Biomass In Long-Term Organic Crop Rotations, Samuel E. Wortman, John L. Lindquist, Milton J. Haar, Charles A. Francis Jan 2010

Increased Weed Diversity, Density And Above-Ground Biomass In Long-Term Organic Crop Rotations, Samuel E. Wortman, John L. Lindquist, Milton J. Haar, Charles A. Francis

Department of Agronomy and Horticulture: Faculty Publications

While weed management is consistently a top priority among farmers, there is also growing concern for the conservation of biodiversity. Maintaining diverse weed communities below bioeconomic thresholds may provide ecosystem services for the crop and the surrounding ecosystem. This study was conducted to determine if weed diversity, density and biomass differ within and among organic and conventional crop rotations. In 2007 and 2008, we sampled weed communities in four long-term crop rotations near Mead, Nebraska, United States using seedbank analyses (elutriation and greenhouse emergence) and above-ground biomass sampling. Two conventional crop rotations consisted of a corn (Zea mays) …


Ecology Of Mayfly (Insecta : Ephemeroptera) Nymphs In Three Streams Of Ulu Gombak Forest Reserve, Peninsular Malaysia., Sree Gayathree Selvantharan Jan 2010

Ecology Of Mayfly (Insecta : Ephemeroptera) Nymphs In Three Streams Of Ulu Gombak Forest Reserve, Peninsular Malaysia., Sree Gayathree Selvantharan

Student Works (2010-2019)

Three sampling stations with various environmental quality gradients in Ulu Gombak Forest Reserve, Selangor, Peninsular Malaysia were selected in this study: Sungai Gombak, Anak Sungai Gombak and Sungai Batu 19. A total of six genera of ephemeropteran nymphs were found from the mentioned streams: Baetis sp. (Baetidae) (232), Thalerosphyrus sp. (Heptageniidae) (925), Campsoneuria sp. (Heptageniidae) (1432), Neurocaenis sp. (Tricorythidae) (471), Platybaetis sp. (Baetidae) (12) and Prosopistoma sp. (Prosopistomatidae) (1). The most dominant family of ephemeropteran nymphs is Heptageniidae while Neurocaenis sp. (Tricorythidae) were the only nymphs that showed significant difference in abundance between all three streams (p=0.05) using student t-test. …


Isolation And Characterization Of Microsatellite Loci From Wild Striped Snakehead (Channa Striata)., Yasmeen Sameer Mahmoud Salah Jan 2010

Isolation And Characterization Of Microsatellite Loci From Wild Striped Snakehead (Channa Striata)., Yasmeen Sameer Mahmoud Salah

Student Works (2010-2019)

Channa striata (better known as Haruan in Malaysia), is a freshwater species that is well known as a protein source and also for its pharmaceutical value among wounded patients. Unfortunately stocks have declined over the past few years, thus an in-depth study is needed to rule out genetic irregularities that might be causing the slow extinction of the species. This study was conducted for the purpose of studying the population diversity and genetic structure of Channa striata (striped snakehead) in seven different locations around the peninsular of Malaysia. Microsatellite markers were isolated and identified using Random Amplified Microsatellite (RAM) primers. …


Isolation And Characterization Of Actinobacteria From Soil Samples Of Signy Island, Antarctica., Pan Shing Yi Jan 2010

Isolation And Characterization Of Actinobacteria From Soil Samples Of Signy Island, Antarctica., Pan Shing Yi

Student Works (2010-2019)

In this study, ninety five actinobacterial isolates were cultured from seven soils samples collected on Signy Island, maritime Antarctica. Nine different isolation media were used and the isolates plates were incubated at 15ºC for up to four months. SCN agar serves as the better media for the isolation of actinobacteria. Presence of actinobacteria in the soil samples was also detected using PCR with actinobacteria specific primers. The actinobacterial isolates were characterized based on morphology (Gram-stain, coverslip examination, colour grouping), analysis of DAP isomers, antibacterial activities and by molecular methods (ARDRA, analysis of 16S rRNA sequence and detection of NRPS gene). …


Application Of Solid-Phase Microextraction For The Analysis Of Polycyclic Aromatic Hydrocarbons In Hyophila Involuta As A Natural Biosensor., Elizabeth Fong Yew Mei Jan 2010

Application Of Solid-Phase Microextraction For The Analysis Of Polycyclic Aromatic Hydrocarbons In Hyophila Involuta As A Natural Biosensor., Elizabeth Fong Yew Mei

Student Works (2010-2019)

This work describes a simple and rapid extraction method by using solid-phase microextraction (SPME) technique for the determination of polycyclic aromatic hydrocarbons (PAHs) from mosses of Hyophila involuta which is found commonly and abundantly throughout Peninsular Malaysia. Mosses have been chosen in our study since they are effective bioindicators as they take up nutrients and pollutants directly from the atmosphere and they have no root system. These properties make them ideal for evaluating pollutant levels in the air over a monitored period of time. This study has been developed and optimized based on the extraction of the analytes of interest …