Dairy Science Commons^™

A Molecularly Engineered Antiviral Banana Lectin Inhibits Fusion And Is Efficacious Against Influenza Virus Infection In Vivo, Evelyn M. Covés-Datson, Steven R. King, Maureen Legendre, Auroni Gupta, Susana M. Chan, Emily Gitlin, Vikram V. Kulkarni, Jezreel Pantaleón García, Donald F. Smee, Elke Lipka, Scott E. Evans, E. Bart Tarbet, Akira Ono, David M. Markovitz

Animal, Dairy, and Veterinary Science Faculty Publications

There is a strong need for a new broad-spectrum antiinfluenza therapeutic, as vaccination and existing treatments are only moderately effective. We previously engineered a lectin, H84T banana lectin (H84T), to retain broad-spectrum activity against multiple influenza strains, including pandemic and avian, while largely eliminating the potentially harmful mitogenicity of the parent compound. The amino acid mutation at position 84 from histidine to threonine minimizes the mitogenicity of the wild-type lectin while maintaining antiinfluenza activity in vitro. We now report that in a lethal mouse model H84T is indeed nonmitogenic, and both early and delayed therapeutic administration of H84T intraperitoneally are …

Zika Virus Infection Causes Temporary Paralysis In Adult Mice With Motor Neuron Synaptic Retraction And Evidence For Proximal Peripheral Neuropathy, John D. Morrey, Alexandre L. R. Oliveira, Hong Wang, Katherine Zukor, Mateus Vidigal De Castro, Venkatraman Siddharthan

Animal, Dairy, and Veterinary Science Faculty Publications

Clinical evidence is mounting that Zika virus can contribute to Guillain-Barré syndrome which causes temporary paralysis, yet the mechanism is unknown. We investigated the mechanism of temporary acute flaccid paralysis caused by Zika virus infection in aged interferon αβ-receptor knockout mice used for their susceptibility to infection. Twenty-five to thirty-five percent of mice infected subcutaneously with Zika virus developed motor deficits including acute flaccid paralysis that peaked 8-10 days after viral challenge. These mice recovered within a week. Despite Zika virus infection in the spinal cord, motor neurons were not destroyed. We examined ultrastructures of motor neurons and synapses by …

Docosahexaenoic Acid Consumption Impedes Early Interferon- And Chemokine-Related Gene Expression While Suppressing Silica-Triggered Flaring Of Murine Lupus, Abby D. Benninghoff, Melissa A. Bates, Preeti S. Chauhan, Kathryn A. Wierenga, Kristen N. Gilley, Andrij Holian, Jack R. Harkema, James J. Pestka

Animal, Dairy, and Veterinary Science Faculty Publications

Exposure of lupus-prone female NZBWF1 mice to respirable crystalline silica (cSiO₂), a known human autoimmune trigger, initiates loss of tolerance, rapid progression of autoimmunity, and early onset of glomerulonephritis. We have previously demonstrated that dietary supplementation with the ω-3 polyunsaturated fatty acid docosahexaenoic acid (DHA) suppresses autoimmune pathogenesis and nephritis in this unique model of lupus flaring. In this report, we utilized tissues from prior studies to test the hypothesis that DHA consumption interferes with upregulation of critical genes associated with cSiO₂-triggered murine lupus. A NanoString nCounter platform targeting 770 immune-related genes was used to assess …

Vascular Leak And Hypercytokinemia Associated With Severe Fever With Thrombocytopenia Syndrome Virus Infection In Mice, Jonna B. Westover, Brady T. Hickerson, Arnaud J. Van Wettere, Brett L. Hurst, Jacqueline P. Kurz, Ashley Dagley, Petra Wülfroth, Takashi Komeno, Yousuke Furuta, Thomas Steiner, Brian B. Gowen

Animal, Dairy, and Veterinary Science Faculty Publications

Severe fever with thrombocytopenia syndrome (SFTS) is an emerging viral hemorrhagic fever (VHF) endemic to China, South Korea, Japan, and Vietnam. Here we characterize the pathogenesis and natural history of disease in IFNAR^-/- mice challenged with the HB29 strain of SFTS virus (SFTSV) and demonstrate hallmark features of VHF such as vascular leak and high concentrations of proinflammatory cytokines in blood and tissues. Treatment with FX06, a natural plasmin digest product of fibrin in clinical development as a treatment for vascular leak, reduced vascular permeability associated with SFTSV infection but did not significantly improve survival outcome. Further studies are …

Function And Transcriptional Regulation Of Bovine Torc2 Gene In Adipocytes: Roles Of C/Ebpγ, Xbp1, Insm1 And Znf263, Rajwali Khan, Sayed Haidar Abbas Raza, Zainaguli Junjvlieke, Wang Xiaoyu, Matthew D. Garcia, Ibrahim Elsaeid Elnour, Wang Hongbao, Zan Linsen

Animal, Dairy, and Veterinary Science Faculty Publications

The TORC2 gene is a member of the transducer of the regulated cyclic adenosine monophosphate (cAMP) response element binding protein gene family, which plays a key role in metabolism and adipogenesis. In the present study, we confirmed the role of TORC2 in bovine preadipocyte proliferation through cell cycle staining flow cytometry, cell counting assay, 5-ethynyl-20 -deoxyuridine staining (EdU), and mRNA and protein expression analysis of proliferation-related marker genes. In addition, Oil red O staining analysis, immunofluorescence of adiponectin, mRNA and protein level expression of lipid related marker genes confirmed the role of TORC2 in the regulation of bovine adipocyte differentiation. …

Basal Diet Determined Long-Term Composition Of The Gut Microbiome And Mouse Phenotype To A Greater Extent Than Fecal Microbiome Transfer From Lean Or Obese Human Donors, Daphne Michelle Rodriguez Jimenez, Abby D. Benninghoff, Niklas D.J. Aardema, Sumira Phatak, Korry J. Hintze

Animal, Dairy, and Veterinary Science Faculty Publications

The Western dietary pattern can alter the gut microbiome and cause obesity and metabolic disorders. To examine the interactions between diet, the microbiome, and obesity, we transplanted gut microbiota from lean or obese human donors into mice fed one of three diets for 22 weeks: (1) a control AIN93G diet; (2) the total Western diet (TWD), which mimics the American diet; or (3) a 45% high-fat diet-induced obesity (DIO) diet. We hypothesized that a fecal microbiome transfer (FMT) from obese donors would lead to an obese phenotype and aberrant glucose metabolism in recipient mice that would be exacerbated by consumption …

Zika Virus Associated Pathology And Antigen Presence In The Testicle In The Absence Of Sexual Transmission During Subacute To Chronic Infection In A Mouse Model, Chad S. Clancy, Arnaud J. Van Wettere, John D. Morrey, Justin G. Julander

Animal, Dairy, and Veterinary Science Faculty Publications

Zika virus (ZIKV) is an arboviral infection that has been shown to be sexually transmitted. The study outlined herein aims to determine if accessory sex glands and epididymal epithelial cells are sources of viral persistence in subacute and chronic ZIKV infection, and if infection of these organs is important in sexual transmission during long-term (chronic) infection. Male interferon type I receptor knockout (Ifnar−/−) mice were challenged with ZIKV and reproductive tissues were harvested 14 and 35 days post infection (DPI) for inoculation studies and 14, 35 and 70 DPI for histopathology. Artificial insemination fluid derived from epididymal …

Incomplete Urethral Duplication Associated With A Dermoid Cyst Within A Vascular Hamartoma In A Female Dog, Chad S. Clancy, Gordon A. Hullinger, Arnaud J. Van Wettere

Animal, Dairy, and Veterinary Science Faculty Publications

A seven-year-old spayed female Labrador retriever presented for necropsy following an acute history of thrombocytopenia, anemia, leukocytosis and abdominal effusion. A 2 × 3 × 10 cm, cylindrical to tubular, mottled red-to-tan mass extended from the caudal pelvic cavity caudally and ventrally under the dermis along the caudal aspect of the left pelvic limb adjacent to the semimembranosus and semitendinosus musculature. Histologic examination of the mass revealed a singular central lumen lined by urothelium that multifocally transitioned into non-keratinizing, stratified squamous epithelium associated with few hair follicles and sweat glands. The lumen was surrounded by a dense collagenous stroma containing …

Lack Of Selective Resistance Of Influenza A Virus In Presence Of Host-Targeted Antiviral, Uv-4b, Kelly L. Warfield, Kaitlyn R. Schaaf, Lisa Evans Dewald, Kevin B. Spurgers, Wei Wang, Eric Stavale, Michelle Mendenhall, Meghan H. Shilts, Timothy B. Stockwell, Dale L. Barnard, Urban Ramstedt, Suman R. Das

Animal, Dairy, and Veterinary Science Faculty Publications

Development of antiviral drug resistance is a continuous concern for viruses with high mutation rates such as influenza. The use of antiviral drugs targeting host proteins required for viral replication is less likely to result in the selection of resistant viruses than treating with direct-acting antivirals. The iminosugar UV-4B is a host-targeted glucomimetic that inhibits endoplasmic reticulum α-glucosidase I and II enzymes resulting in improper glycosylation and misfolding of viral glycoproteins. UV-4B has broad-spectrum antiviral activity against diverse viruses including dengue and influenza. To examine the ability of influenza virus to develop resistance against UV-4B, mouse-adapted influenza virus was passaged …

Genome To Phenome: Improving Animal Health, Production, And Well-Being – A New Usda Blueprint For Animal Genome Research 2018–2027, Caird Rexroad, Jeffrey Vallet, Lakshmi Kumar Matukumalli, James Reecy, Derek Bickhart, Harvey Blackburn, Mark Boggess, Hans Cheng, Archie Clutter, Noelle Cockett, Catherine Ernst, Janet E. Fulton, John Liu, Joan Lunney, Holly Neibergs, Et Al.

Animal, Dairy, and Veterinary Science Faculty Publications

In 2008, a consortium led by the Agricultural Research Service (ARS) and the National Institute for Food and Agriculture (NIFA) published the “Blueprint for USDA Efforts in Agricultural Animal Genomics 2008–2017,” which served as a guiding document for research and funding in animal genomics. In the decade that followed, many of the goals set forth in the blueprint were accomplished. However, several other goals require further research. In addition, new topics not covered in the original blueprint, which are the result of emerging technologies, require exploration. To develop a new, updated blueprint, ARS and NIFA, along with scientists in the …

Altered Gene Response To Aflatoxin B1 In The Spleens Of Susceptible And Resistant Turkeys, Kent M. Reed, Kristelle M. Mendoza, Roger A. Coulombe Jr.

Animal, Dairy, and Veterinary Science Faculty Publications

Susceptibility and/or resistance to aflatoxin B₁ (AFB₁) is a threshold trait governed principally by glutathione S transferase (GST)-mediated detoxification. In poultry, domesticated turkeys are highly sensitive to AFB₁, most likely due to dysfunction in hepatic GSTs. In contrast, wild turkeys are comparatively resistant to aflatoxicosis due to the presence of functional hepatic GSTAs and other possible physiological and immunological interactions. The underlying genetic basis for the disparate GST function in turkeys is unknown as are the broader molecular interactions that control the systemic response. This study quantifies the effects of dietary AFB₁ on gene …

Efficacy Of A Ml336 Derivative Against Venezuelan And Eastern Equine Encephalitis Viruses, Colleen B. Jonsson, Xufeng Cao, Jasper Lee, Jon D. Gabbard, Yong-Kyu Chu, Elizabeth A. Fitzpatrick, Justin G. Julander, Dong-Hoon Chung, Jennifer Stabenow, Jennifer E. Golden

Animal, Dairy, and Veterinary Science Faculty Publications

Currently, there are no licensed human vaccines or antivirals for treatment of or prevention from infection with encephalitic alphaviruses. Because epidemics are sporadic and unpredictable, and endemic disease is common but rarely diagnosed, it is difficult to identify all populations requiring vaccination; thus, an effective post-exposure treatment method is needed to interrupt ongoing outbreaks. To address this public health need, we have continued development of ML336 to deliver a molecule with prophylactic and therapeutic potential that could be relevant for use in natural epidemics or deliberate release scenario for Venezuelan equine encephalitis virus (VEEV). We report findings from in vitro …

Mapping Of Dynamic Transcriptome Changes Associated With Silica-Triggered Autoimmune Pathogenesis In The Lupus-Prone Nzbwf1 Mouse, Melissa A. Bates, Abby D. Benninghoff, Kristen N. Gilley, Andrij Holian, Jack R. Harkema, James J. Pestka

Animal, Dairy, and Veterinary Science Faculty Publications

Crystalline silica (cSiO₂) is a widely recognized environmental trigger of autoimmune disease. In the lupus-prone female NZBWF1 mouse, airway exposure to cSiO₂ triggers pulmonary ectopic lymphoid neogenesis, systemic autoantibody elevation, and glomerulonephritis. Here we tested the hypothesis that upregulation of adaptive immune function genes in the lung precedes cSiO₂-triggering of autoimmune disease in this model. The study include three groups of mice, as follows: (1) necropsied 1 d after a single intranasal instillation of 1 mg cSiO₂ or vehicle, (2) necropsied 1 d after four weekly single instillations of 1 mg cSiO₂ or …

Generation Of H7n9-Specific Human Polyclonal Antibodies From A Transchromosomic Goat (Caprine) System, Hua Wu, Zhiqiang Fan, Michelle Brandsrud, Qinggang Meng, Molly Bobbitt, Misha Regouski, Rusty Stott, Alexis Sweat, Jackelyn Crabtree, Robert J. Hogan, Ralph A. Tripp, Zhongde Wang, Irina A. Polejaeva, Eddie J. Sullivan

Animal, Dairy, and Veterinary Science Faculty Publications

To address the unmet needs for human polyclonal antibodies both as therapeutics and diagnostic reagents, building upon our previously established transchromosomic (Tc) cattle platform, we report herein the development of a Tc goat system expressing human polyclonal antibodies in their sera. In the Tc goat system, a human artificial chromosome (HAC) comprising the entire human immunoglobulin (Ig) gene repertoire in the germline configuration was introduced into the genetic makeup of the domestic goat. We achieved this by transferring the HAC into goat fetal fibroblast cells followed by somatic cell nuclear transfer for Tc goat production. Gene and protein expression analyses …

Human Polyclonal Antibodies Produced From Transchromosomal Bovine Provides Prophylactic And Therapeutic Protections Against Zika Virus Infection In Stat2 Ko Syrian Hamsters, Venkatraman Siddharthan, Jinxin Miao, Arnaud J. Van Wettere, Rong Li, Hua Wu, Eddie Sullivan, Jinan Jiao, Jay W. Hooper, David Safronetz, John D. Morrey, Justin G. Julander, Zhongde Wang

Animal, Dairy, and Veterinary Science Faculty Publications

Zika virus (ZIKV) infection can cause severe congenital diseases, such as microcephaly, ocular defects and arthrogryposis in fetuses, and Guillain–Barré syndrome in adults. Efficacious therapeutic treatments for infected patients, as well as prophylactic treatments to prevent new infections are needed for combating ZIKV infection. Here, we report that ZIKV-specific human polyclonal antibodies (SAB-155), elicited in transchromosomal bovine (TcB), provide significant protection from infection by ZIKV in STAT2 knockout (KO) golden Syrian hamsters both prophylactically and therapeutically. These antibodies also prevent testicular lesions in this hamster model. Our data indicate that antibody-mediated immunotherapy is effective in treating ZIKV infection. Because suitable …

Differential Transcriptome Responses To Aflatoxin B1 In The Cecal Tonsil Of Susceptible And Resistant Turkeys, Kent M. Reed, Kristelle M. Mendoza, Roger A. Coulombe Jr.

Animal, Dairy, and Veterinary Science Faculty Publications

The nearly-ubiquitous food and feed-borne mycotoxin aflatoxin B₁ (AFB₁) is carcinogenic and mutagenic, posing a food safety threat to humans and animals. One of the most susceptible animal species known and thus a good model for characterizing toxicological pathways, is the domesticated turkey (DT), a condition likely due, at least in part, to deficient hepatic AFB₁-detoxifying alpha-class glutathione S-transferases (GSTAs). Conversely, wild turkeys (Eastern wild, EW) are relatively resistant to the hepatotoxic, hepatocarcinogenic and immunosuppressive effects of AFB₁ owing to functional gene expression and presence of functional hepatic GSTAs. This study was designed to …

A Potent Lassa Virus Antiviral Targets An Arenavirus Virulence Determinant, Ikenna G. Madu, Megan Files, Dima N. Gharaibeh, Amy L. Moore, Kieh-Hoon Jung, Brian B. Gowen, Dongcheng Dai, Kevin F. Jones, Shanthakumar R. Tyavanagimatt, James R. Burgeson, Marcus J. Korth, Kristin M. Bedard, Shawn P. Iadonato, Sean M. Amberg

Animal, Dairy, and Veterinary Science Faculty Publications

Arenaviruses are a significant cause of hemorrhagic fever, an often-fatal disease for which there is no approved antiviral therapy. Lassa fever in particular generates high morbidity and mortality in West Africa, where the disease is endemic, and a recent outbreak in Nigeria was larger and more geographically diverse than usual. We are developing LHF-535, a small-molecule viral entry inhibitor that targets the arenavirus envelope glycoprotein, as a therapeutic candidate for Lassa fever and other hemorrhagic fevers of arenavirus origin. Using a lentiviral pseudotype infectivity assay, we determined that LHF-535 had sub-nanomolar potency against the viral envelope glycoproteins from all Lassa …

Pathogenesis Of Rift Valley Fever Virus Aerosol Infection In Stat2 Knockout Hamsters, Brady T. Hickerson, Jonna B. Westover, Arnaud J. Van Wettere, Johanna D. Rigas, Jinxin Miao, Bettina L. Conrad, Neil E. Motter, Zhongde Wang, Brian B. Gowen

Animal, Dairy, and Veterinary Science Faculty Publications

Rift Valley fever virus (RVFV) is an emerging pathogen capable of causing severe disease in livestock and humans and can be transmitted by multiple routes including aerosol exposure. Several animal models have been developed to gain insight into the pathogenesis associated with aerosolized RVFV infection, but work with these models is restricted to high containment biosafety level (BSL) laboratories limiting their use for antiviral and vaccine development studies. Here, we report on a new RVFV inhalation infection model in STAT2 KO hamsters exposed to aerosolized MP-12 vaccine virus by nose-only inhalation that enables a more accurate delivery and measurement of …

Coitus-Free Sexual Transmission Of Zika Virus In A Mouse Model, Chad S. Clancy, Arnaud J. Van Wettere, John D. Morrey, Justin G. Julander

Animal, Dairy, and Veterinary Science Faculty Publications

Zika virus (ZIKV) is an arboviral infection that may be sexually transmitted. The present study aims to determine if accessory sex glands are a potential source of infectious virus and important in sexual transmission. Male interferon type I receptor knockout (Ifnar^−/−) mice were challenged subcutaneously with a Puerto Rican ZIKV isolate. Reproductive tissues were harvested seven days after viral challenge and artificial insemination fluid derived from epididymis or homogenized accessory sex glands (seminal plasma) was obtained. Naïve interferon type I and II receptor knockout (AG129) females were pretreated with progesterone, and inoculated intravaginally with either epididymal flush or …

A Novel Cancer Syndrome Caused By Kcnq1-Deficiency In The Golden Syrian Hamster, Rong Li, Jinxin Miao, Alexandru-Flaviu Tabaran, M. Gerard O'Sullivan, Kyle J. Anderson, Patricia M. Scott, Zhongde Wang, Robert T. Cormier

Animal, Dairy, and Veterinary Science Faculty Publications

BACKGROUND: The golden Syrian hamster is an emerging model organism. To optimize its use, our group has made the first genetically engineered hamsters. One of the first genes that we investigated is KCNQ1 which encodes for the KCNQ1 potassium channel and also has been implicated as a tumor suppressor gene.

MATERIALS AND METHODS: We generated KCNQ1 knockout (KO) hamsters by CRISPR/Cas9­-mediated gene targeting and investigated the effects of KCNQ1­-deficiency on tumorigenesis.

RESULTS: By 70 days of age seven of the eight homozygous KCNQ1 KOs used in this study began showing signs of distress, and on necropsy six of the seven …

Intramammary Infusion Of Casein Hydrolysate For Involution Of Single Mastitic Mammary Quarters Elevating Cow-Level Somatic Cell Count, Justine Britten, Kerry A. Rood, David J. Wilson

Animal, Dairy, and Veterinary Science Faculty Publications

Mastitis in a single quarter can cause high somatic cell counts (SCC), clinical mastitis, and death in dairy cows. Currently, management of these mastitic quarters presents a problem for the dairy industry. Casein hydrolysate (CH) is an intramammary (IMM) infusion treatment reported to induce mammary involution. The primary objectives of this study were to investigate whether IMM CH treatment of single high SCC quarters, followed by cessation of quarter milk production for the remainder of lactation, was effective in reducing cow–level SCC and whether that quarter resumed milk production following calving. Three treatment groups were used: CH, non-hydrolyzed casein (NHC), …

Early Events In Japanese Encephalitis Virus Infection: Viral Entry, Sang-Im Yun, Young-Min Lee

Animal, Dairy, and Veterinary Science Faculty Publications

Japanese encephalitis virus (JEV), a mosquito-borne zoonotic flavivirus, is an enveloped positive-strand RNA virus that can cause a spectrum of clinical manifestations, ranging from mild febrile illness to severe neuroinvasive disease. Today, several killed and live vaccines are available in different parts of the globe for use in humans to prevent JEV-induced diseases, yet no antivirals are available to treat JEV-associated diseases. Despite the progress made in vaccine research and development, JEV is still a major public health problem in southern, eastern, and southeastern Asia, as well as northern Oceania, with the potential to become an emerging global pathogen. In …

Functional Genomics And Immunologic Tools: The Impact Of Viral And Host Genetic Variations On The Outcome Of Zika Virus Infection, Sang-Im Yun, Byung-Hak Song, Jordan C. Frank, Justin G. Julander, Aaron L. Olsen, Irina A. Polejaeva, Christopher J. Davies, Kenneth L. White, Young-Min Lee

Animal, Dairy, and Veterinary Science Faculty Publications

Zika virus (ZIKV) causes no-to-mild symptoms or severe neurological disorders. To investigate the importance of viral and host genetic variations in determining ZIKV infection outcomes, we created three full-length infectious cDNA clones as bacterial artificial chromosomes for each of three spatiotemporally distinct and genetically divergent ZIKVs: MR-766 (Uganda, 1947), P6-740 (Malaysia, 1966), and PRVABC-59 (Puerto Rico, 2015). Using the three molecularly cloned ZIKVs, together with 13 ZIKV region-specific polyclonal antibodies covering nearly the entire viral protein-coding region, we made three conceptual advances: (i) We created a comprehensive genome-wide portrait of ZIKV gene products and their related species, with several previously …

Immunogenicity And Protection After Vaccination With A Modified Vaccinia Virus Ankara-Vectored Yellow Fever Vaccine In The Hamster Model, Justin G. Julander, Marco Testori, Cédric Cheminay, Ariane Volkmann

Animal, Dairy, and Veterinary Science Faculty Publications

The highly efficacious live-attenuated 17D yellow fever (YF) vaccine is occasionally associated with rare life-threatening adverse events. Modified vaccinia virus Ankara (MVA), a non-replicating poxvirus, has been used as a vaccine platform to safely deliver various antigens. A MVA-based YF vaccine (MVA-BN-YF) was tested with and without a non-mineral oil adjuvant in a hamster model of lethal YF disease and protective efficacy of this vaccine was compared with the 17D vaccine. The vaccine candidate MVA-BN-YF generated a protective response in hamsters infected with YFV that was comparable to protection by the live 17D vaccine. Similar levels of neutralizing antibody were …

A Non-Recoverable Hybridoma Limits The Production Of Monoclonal Antibodies Against Bovine Trophoblast Non-Classical Nc3*00101 Protein, Parveen Parasar, Amanda Wilhelm, William C. Davis, Kenneth L. White, Christopher J. Davies

Animal, Dairy, and Veterinary Science Faculty Publications

The non-classical MHC class I (MHC-Ib) proteins are important modulators of immune system during pregnancy favoring survival of the fetus. Contrary to ubiquitously expressed classical MHC-I proteins, MHC-Ib proteins are oligomorphic, and expressed in specific cell/tissue types thus minimizing maternal immune-mediated rejection of fetal-allograft and a successful pregnancy. A unique characteristic of MHC-Ib glycoproteins is expression of surface and soluble isoforms due to alternative splicing phenomenon. Bovine fetal trophoblast cells, during the third trimester of pregnancy, express non-classical bovine leukocyte antigen class Ib (BoLA-Ib) antigens. BoLA-NC3*00101, is a non-classical class I allele from cattle AH11 …

Urine Volume And Nitrogen Excretion Are Altered By Feeding Birdsfoot Trefoil Compared With Alfalfa In Lactating Dairy Cows, Mohammad Ghelichkhan, Jong-Su Eun, Rachael G. Christensen, Rusty D. Stott, Jennifer W. Macadam

Animal, Dairy, and Veterinary Science Faculty Publications

Legumes that contain condensed tannins may have lower ruminal protein degradation than alfalfa. The present study investigated the effects of feeding birdsfoot trefoil (Lotus corniculatus L.) hay on lactational performance and N utilization and excretion. Eight multiparous Holstein cows in midlactation (150 ± 22.3 d-in-milk) were randomly assigned to 2 treatments [alfalfa hay-based total mixed ration (AHT) or birdsfoot trefoil hay-based total mixed ration (BHT)] in a crossover design with 2 experimental periods. Each experimental period lasted 17 d (14 d of adaptation and 3 d of sampling and total collection). Hays comprised approximately 50% …

Consequences Of In Utero Exposure To Zika Virus In Offspring Of Ag129 Mice, Justin G. Julander, Venkatraman Siddharthan, Albert H. Park, Elizabeth S. Preston, Pranav Mathur, Michael Bertolio, Hong Wang, Katherine Zukor, Arnaud J. Van Wettere, Donal G. Sinex, John D. Morrey

Animal, Dairy, and Veterinary Science Faculty Publications

Zika virus (ZIKV) can cause various diseases in offspring after congenital infection. The purpose of this study was to identify disease phenotypes in pups exposed to ZIKV in utero. Female interferon-α/β, -γ receptor knockout mice (AG129) were infected intraperitoneally with ZIKV 7.5 days’ post coitus (dpc). Viral RNA, antigen and infectious virus were detected in some, but not all, maternal and fetal tissues at various times during gestation. Fetuses of infected dams had significant intrauterine growth restriction (IUGR), which was more pronounced as females neared parturition. Pups born to infected dams were significantly smaller and had significantly shortened skull …

Large Animal Genetic Engineering Summit 2018 Proceedings, Utah State University

Animal, Dairy, and Veterinary Science Faculty Publications

It is our great pleasure to welcome you to the second Large Animal Genetic Engineering (LAGE) Summit in beautiful Park City, Utah. We hope that you enjoy the meeting and take advantage of your free time to get out and see what Park City has to offer. Our objectives for the summit are twofold: (1) to provide updates on genetic engineering technologies, including genome editing, and their biomedical and agricultural applications in academia and industry; and (2) to engage federal agencies, industry representatives and academic researchers in a policy development forum. We hope that this year’s meeting will build on …

Characterization Of An N-Terminal Non-Core Domain Of Rag1 Gene Disrupted Syrian Hamster Model Generated By Crispr Cas9, Jinxin Miao, Baoling Ying, Rong Li, Ann E. Tollefson, Jacqueline F. Spencer, William S.M. Wold, Seok-Hwan Song, Il-Keun Kong, Karoly Toth, Yaohe Wang, Zhongde Wang

Animal, Dairy, and Veterinary Science Faculty Publications

The accumulating evidence demonstrates that Syrian hamsters have advantages as models for various diseases. To develop a Syrian hamster (Mesocricetus auratus) model of human immunodeficiency caused by RAG1 gene mutations, we employed the CRISPR/Cas9 system and introduced an 86-nucleotide frameshift deletion in the hamster RAG1 gene encoding part of the N-terminal non-core domain of RAG1. Histological and immunohistochemical analyses demonstrated that these hamsters (referred herein as RAG1-86nt hamsters) had atrophic spleen and thymus, and developed significantly less white pulp and were almost completely devoid of splenic lymphoid follicles. The RAG1-nt86 hamsters had barely detectable CD3+ and CD4+ T cells. The …

Fetal Demise And Failed Antibody Therapy During Zika Virus Infection Of Pregnant Macaques, Diogo M. Magnani, Thomas F. Rogers, Nicholas J. Maness, Nathan D. Grubaugh, Nathan Beutler, Varian K. Bailey, Lucas Gonzalez-Nieto, Martin J. Gutman, Núria Pedreño-Lopez, Jaclyn M. Kwal, Michael J. Ricciardi, Tereance A. Myers, Justin G. Julander, Rudolf P. Bohm, Margaret H. Gilbert, Faith Schiro, Pyone P. Aye, Robert V. Blair, Mauricio A. Martins, Kathrine P. Falkenstien, Amitinder Kaur, Christine L. Curry, Esper G. Kallas, Ronald C. Desrosiers, Pascal J. Goldschmidt-Clermont, Stephen S. Whitehead, Kristian G. Andersen, Myrna C. Bonaldo, Andrew A. Lackner, Antonito T. Panganiban, Dennis R. Burton, David I. Watkins

Animal, Dairy, and Veterinary Science Faculty Publications

Zika virus (ZIKV) infection of pregnant women is associated with pathologic complications of fetal development. Here, we infect pregnant rhesus macaques (Macaca mulatta) with a minimally passaged ZIKV isolate from Rio de Janeiro, where a high rate of fetal development complications was observed. The infection of pregnant macaques with this virus results in maternal viremia, virus crossing into the amniotic fluid (AF), and in utero fetal deaths. We also treated three additional ZIKV-infected pregnant macaques with a cocktail of ZIKV-neutralizing human monoclonal antibodies (nmAbs) at peak viremia. While the nmAbs can be effective in clearing the virus from …