Dairy Science Commons^™

Dermatopathy In Juvenile Angus Cattle Due To Vitamin A Deficiency, Thomas J. Baldwin, Kerry A. Rood, E. Jane Kelly, Jeffery O. Hall

Animal, Dairy, and Veterinary Science Faculty Publications

In juvenile cattle, vitamin A deficiency is reported most commonly as a neurological condition; only rarely are there dermatologic manifestations. In the current study, alopecia, severe epidermal and follicular orthokeratosis, and acanthosis due to hypovitaminosis A are reported in 2 of 32 Angus calves, with a third animal suspected. Affected animals responded to vitamin A supplementation, and no additional calves displayed signs. Vitamin A acts on skin by regulating DNA transcription in keratinocytes, reducing the number of tonofilaments and desmosomes, both involved in cell-to-cell adhesion. Hence, adequate levels of dietary vitamin A are necessary for normal keratinocyte turnover, and deficiencies …

2011 Annual Report, Various Authors

Annual Reports

No abstract provided.

Productivity Studies Utilizing Recombinant Cho Cells In Stirred-Tank Bioreactors: A Comparative Study Between The Pitch-Blade And The Packed-Bed Bioreactor Systems, Taylor Stephen Hatton

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Center for Integrated Biosystems (CIB) at Utah State University proposed to demonstrate the added benefits of bench top stirred-tank bioreactors utilizing the packedbed impeller system in perfusion mode for the production of recombinant proteins. In this study recombinant Chinese Hamster Ovary (CHO) cells were utilized for the production of a secreted protein in two bioreactor types: pitched-blade bioreactor operated in batch mode versus packed-bed bioreactor operated in perfusion mode. Our primary objective was to determine whether the packed-bed bioreactor is superior to a traditional pitched-blade bioreactor for the mass production of cell culture protein products. We hypothesize that the …

In Vitro And In Vivo Comparison Of The Pathogenicity Of Four Influenza Virus Strains, Brett L. Hurst

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Influenza viruses cause between 3 and 5 million cases of respiratory infection each year and are responsible for between 250 and 500 thousand deaths. Two avenues for the treatment and prevention of influenza virus infections are vaccination and antiviral chemotherapy. Prevention is largely accomplished through vaccination. While vaccines remain the preferred method for controlling the spread of influenza, antiviral treatment is important for severe infections caused by new and emerging virus strains. Occasionally new viruses emerge to which the population has no previous immunity. Such was the case when the pandemic H1N1 influenza virus appeared in 2009. When new viruses …

Using Preconditioning Programs As A Management Tool For Value Added Calves, Jessica Crozier, Kerry A. Rood

All Current Publications

This publication discusses using preconditioning programs as a management tool for value added calves.

Low Stress Weaning Alternatives For Cow-Calf Producers: Fenceline-Weaning, Kerry A. Rood

All Current Publications

This publication explains fenceline weaning for calves as a low stress weaning option.

Bovine Viral Diarrhea Beef-Herd Risk Assessment, Kerry A. Rood, C Kim Chapman

All Current Publications

This publication gives insite into understanding where the greatest risk to a herd is, relative to the introduction of bovine viral diarrhea (BVD).

2010 Annual Report, Various Authors

Annual Reports

No abstract provided.

Assessment Of Genome Integrity With Array Cgh Of Cattle Transgenic Cell Lines Produced By Homologous Recombination And Somatic Cell Cloning, G. E. Liu, Y. Hou, J. M. Robl, Y. Kuroiwa, Zhongde Wang

Animal, Dairy, and Veterinary Science Faculty Publications

Transgenic cattle carrying multiple genomic modifications have been produced by serial rounds of somatic cell chromatin transfer (cloning) of sequentially genetically targeted somatic cells. However, cloning efficiency tends to decline with the increase of rounds of cloning. It is possible that multiple rounds of cloning compromise the genome integrity or/and introduce epigenetic errors in the resulting cell lines, rendering a decline in cloning. To test these possibilities, we performed 9 high density array Comparative Genomic Hybridization (CGH) experiments to test the genome integrity in 3 independent bovine transgenic cell lineages generated from genetic modification and cloning. Our plan included the …

Commentary On Immune System Associated Diseases Caused Byviruses: The Role Of Ebv, Dale L. Barnard, J. K. Li

Animal, Dairy, and Veterinary Science Faculty Publications

In her review using the human Epstein–Barr virus (EBV) as an example, Dr. Chen intends to illustrate how host immune systems have evolved in response to pathogens or in many cases co-evolved with pathogens. She discusses why EBV in most people causes what appears to be a self-limiting lymphoproliferative disease (or is it? See her discussion of autoimmune diseases and EBV), but in a very small proportion of individuals the virus causes malignant diseases of severe consequence. She reviews some of the plausible explanations that may include interactions of environmental and host genetic factors resulting in EBV associated malignancies. She …

Phosphorous In Dairy Cattle Diets, Rhonda Miller, Allen Young, Jennifer Major, Lydia Trinca

Agriculture

No abstract provided.

Phosphorous In Dairy Cattle Diets, Rhonda Miller, Allen Young, Jennifer Major, Lydia Trinca

All Current Publications

No abstract provided.

Development Of A New Tacaribearenavirus Infection Model And Its Use To Explore Antiviral Activity Of A Novelaristeromycin Analog, Brian B. Gowen, M. H. Wong, D. Larson, W. Ye, K. H. Jung, E. J. Sefing, Ramona T. Skirpstunas, Donald F. Smee, John D. Morrey, S. W. Schneller

Animal, Dairy, and Veterinary Science Faculty Publications

Background

A growing number of arenaviruses can cause a devastating viral hemorrhagic fever (VHF) syndrome. They pose a public health threat as emerging viruses and because of their potential use as bioterror agents. All of the highly pathogenic New World arenaviruses (NWA) phylogenetically segregate into clade B and require maximum biosafety containment facilities for their study. Tacaribe virus (TCRV) is a nonpathogenic member of clade B that is closely related to the VHF arenaviruses at the amino acid level. Despite this relatedness, TCRV lacks the ability to antagonize the host interferon (IFN) response, which likely contributes to its inability to …

Induction Of Ip-10 By Sars-Cov Infection Of Calu-3cells And Balb/C Mice, Y. Kumaki, C. W. Day, K. W. Bailey, M. H. Wong, M. K. Wandersee, R. Madsen, J. S. Madsen, N. M. Nelson, J. D. Hoopes, J. D. Woolcott, Z. T. Mclean, L. M. Blatt, A. M. Salazar, Dale L. Barnard

Animal, Dairy, and Veterinary Science Faculty Publications

Destruction of the architectural and subsequently the functional integrity of the lung following pulmonary viral infections is attributable to both the extent of pathogen replication and to the host-generated inflammation associated with the recruitment of immune responses. The presence of antigenically disparate pulmonary viruses and the emergence of novel viruses assures the recurrence of lung damage with infection and resolution of each primary viral infection. Thus, there is a need to develop safe broad spectrum immunoprophylactic strategies capable of enhancing protective immune responses in the lung but which limits immune-mediated lung damage. The immunoprophylactic strategy described here utilizes a protein …

Making And Keeping Your Cattle Herd Safe From Bovine Viral Diarrhea (Bvd), Kerry A. Rood, C Kim Chapman, Allen Young

All Current Publications

This publication provides steps for keeping cattle herds safe from Bovine Viral Diarrhea (BVD).

18th Biennial Cheese Industry Conference, Various Authors

Cheese Industry Conference

No abstract provided.

Isolation And Characterization Of Different Aggregates Of Lipid From Bovine Milk, Ankur Jhanwar

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Bovine milk fat globules naturally vary from less than 0.2 µm to 15 µm in diameter. Milk has at least two distinct distributions of fat globules. While the majority (~90%) of globules in milk are of the smaller distribution (average diameter of 0.4 µm), virtually all the fat is carried in the larger globules (average diameter 3.5 µm). This distribution suggests some compositional and/or functional significance might exist between the two populations of fat globules, which may be related to origin of these globules in the lactating cell.

Milk fat globules have a unique structure, composed of a core droplet …

2008 Annual Report, Various Authors

Annual Reports

No abstract provided.

Inducible Bronchus-Associated Lymphoid Tissue Elicited By A Protein Cage Nanoparticle Enhances Protectionin Mice Against Diverse Respiratory Viruses, J. A. Wiley, L. E. Richert, S. D. Swain, A. Harmsen, Dale L. Barnard, T. D. Randall, M. Jutila, T. Douglas, C. Broomell, M. Young, A. Harmsen

Animal, Dairy, and Veterinary Science Faculty Publications

Destruction of the architectural and subsequently the functional integrity of the lung following pulmonary viral infections is attributable to both the extent of pathogen replication and to the host-generated inflammation associated with the recruitment of immune responses. The presence of antigenically disparate pulmonary viruses and the emergence of novel viruses assures the recurrence of lung damage with infection and resolution of each primary viral infection. Thus, there is a need to develop safe broad spectrum immunoprophylactic strategies capable of enhancing protective immune responses in the lung but which limits immune-mediated lung damage. The immunoprophylactic strategy described here utilizes a protein …

Transcriptional Reprogramming Of Gene Expression In Bovine Somatic Cell Chromatin Transfer Embryos, N. Rodriguez-Osorio, Zhongde Wang, G. P. Page, J. M. Robl, E. Memili

Animal, Dairy, and Veterinary Science Faculty Publications

Background Successful reprogramming of a somatic genome to produce a healthy clone by somatic cells nuclear transfer (SCNT) is a rare event and the mechanisms involved in this process are poorly defined. When serial or successive rounds of cloning are performed, blastocyst and full term development rates decline even further with the increasing rounds of cloning. Identifying the "cumulative errors" could reveal the epigenetic reprogramming blocks in animal cloning. Results Bovine clones from up to four generations of successive cloning were produced by chromatin transfer (CT). Using Affymetrix bovine microarrays we determined that the transcriptomes of blastocysts derived from the …

A New Mouse-Adapted Strain Of Sars-Cov As A Lethal Model Forevaluating Antiviral Agents In Vitro And In Vivo, C. W. Day, R. Baric, S. X. Cai, M. Frieman, Y. Kumaki, John D. Morrey, Donald F. Smee, Dale L. Barnard

Animal, Dairy, and Veterinary Science Faculty Publications

Severe acute respiratory syndrome (SARS) is a highly lethal emerging disease caused by coronavirus SARS-CoV. New lethal animal models for SARS were needed to facilitate antiviral research. We adapted and characterized a new strain of SARS-CoV (strain v2163) that was highly lethal in 5–6 week old BALB/c mice. It had nine mutations affecting 10 amino acid residues. Strain v2163 increased IL-1α, IL-6, MIP-1α, MCP-1, and RANTES in mice, and high IL-6 expression correlated with mortality. The infection largely mimicked human disease, but lung pathology lacked hyaline membrane formation. In vitro efficacy against v2163 was shown with known inhihibitors of SARSCoV …

A Recombinant,Infectious Human Parainfluenza Virus Type 3 Expressing The Enhanced Green Fluorescentprotein For Use In High-Throughput Antiviral Assays, J. P. Roth, J. K. Li, Donald F. Smee, John D. Morrey, Dale L. Barnard

Animal, Dairy, and Veterinary Science Faculty Publications

The ability to rescue an infectious, recombinant, negative-stranded, RNA virus from a cDNA clone, has led to new opportunities for measuring viral replication from a viral expressed reporter gene. In this study, the enhanced green fluorescent protein (EGFP) gene was inserted into the human parainfluenza virus type 3 (HPIV-3) antigenome and a recombinant, infectious virus was rescued. Maximum EGFP expression levels, measured by fluorescence, were seen at day 3. Comparison of a three-day, viral expressed EGFP fluorescence assay to a seven-day, neutral red assay, based on complete cell destruction in virus infected MA-104 cells, yielded Z′-factor values of 0.83 and …

Animal Models For The Study Of Influenza Pathogenesis And Therapy, Dale L. Barnard

Animal, Dairy, and Veterinary Science Faculty Publications

Influenza A viruses causes a variety of illnesses in humans. The most common infection, seasonal influenza, is usually a mild, self-limited febrile syndrome, but it can be more severe in infants, the elderly, and immunodeficient persons, in whom it can progress to severe viral pneumonitis or be complicated by bacterial superinfection, leading to pneumonia and sepsis. Seasonal influenza also occasionally results in neurologic complications. Rarely, viruses that have spread from wild birds to domestic poultry can infect humans; such “avian influenza” can range in severity from mild conjunctivitis through the rapidly lethal disease seen in persons infected with the H5N1 …

Antiviral Activities Andphosphorylation Of 5-Halo-2'-Deoxyuridines And N-Methanocarbathymidine In Cells Infectedwith Vaccinia Virus, Donald F. Smee, D. E. Humphreys, B. L. Hurst, Dale L. Barnard

Animal, Dairy, and Veterinary Science Faculty Publications

The Antipoxviral activities and phosphorylation of N-methanocarbathymidine ([N]-MCT) and four 5-halo-2'-deoxyuridines, namely 5-flouro- (FdU), 5-chloro- (CldU), 5-bromo- (BrdU), and 5-iodo- (IdU) derivatives were explored.

Is The Anti-Psychotic, 10-(3-(Dimethylamino)Propyl)Phenothiazine (Promazine), A Dangerous Drug With Which To Treatsars Infections? Efficacy Of Promazine On Sars-Cov Replication In A Mouse Model, Dale L. Barnard, C. W. Day, K. Bailey, M. Heiner, R. Montgomery, L. Lauridsen, K. H. Jung, P. K. Chan, J. K. Li, R. W. Sidwell

Animal, Dairy, and Veterinary Science Faculty Publications

Phenothiazine and derivatives were tested for inhibition of SARS-CoV replication. Phenothiazine slightly inhibited SARS-CoV replication in a neutral red (NR) uptake assay. Adding a propylamino group to give promazine reduced virus yields (VYR assay) with an EC90 = 8.3 ± 2.8 μM, but without selectivity. Various substitutions in the basic phenothiazine structure did not promote efficacy. Phenazine ethosulfate was the most potent compound by VYR assay (EC90 = 6.1 ± 4.3 μM). All compounds were toxic (IC50 = 6.6–74.5 μM) except for phenoxathiin (IC50 = 858 ± 208 μM) and 10-(alpha-diethylamino-propionyl) phenothiazine·HCl (IC50 = 195 ± 71.2 μM). Consequently, none …

Youth Competitors Gain Benefits From Horse Show Judges' Training Program, Pat Evans, S. Mckendrick, J. Wesley, J. Smith

Animal, Dairy, and Veterinary Science Faculty Publications

This article details how youth benefit from a horse show judges’ training program. While judging is subjective, with training, judges can be more consistent in their placings, with the better horses and/or riders being placed at the top of the class. With qualified judges, youth can be more confident that they will be evaluated in a fair and consistent manner during each show all season long.

Interferon Alfacon 1 Inhibits Sars-Covinfection In Human Bronchial Epithelial Calu-3 Cells, Y. Kumaki, C. W. Day, M. K. Wandersee, B. P. Schow, J. S. Madsen, D. Grant, J. P. Roth, Donald F. Smee, L. M. Blatt, Dale L. Barnard

Animal, Dairy, and Veterinary Science Faculty Publications

The primary targets for SARS-CoV infection are the epithelial cells in the respiratory and intestinal tract. The angiotensin-converting enzyme 2 (ACE-2) has been identified as a functional receptor for SARS-CoV. ACE-2 has been shown to be expressed at the apical domain of polarized Calu-3 cells. In this report, interferon alfacon 1 was examined for inhibitory activities against SARS-CoV on human lung carcinoma epithelial Calu-3 cell line and the other three African green monkey kidney epithelial cell lines. Interferon alfacon 1 demonstrated significant antiviral activity in neutral red uptake assay and virus yield reduction assay. The data might provide an important …

Investigating The Role Of Transcription Factor Ap-2�� In Reproduction, Anne Rachelle Howlett

Undergraduate Honors Capstone Projects

The proper formation of gametes is critical for the propagation of species and for the fertility of the individual. The molecular pathways involved in gamete formation remains elusive, therefore, identification of genes involved is an important prerequisite to further our understanding of reproduction. This research will improve infertility treatments and prevention methods in animals and humans. We created mutants that lacked AP-2�� transcription factor after the mouse had entered meiosis and initiated folliculogenesis. The AP-2�� mutants were still fertile and phenotypically similar to normal mice that expressed AP-2�� transcription factor. We also produced mutants that lacked AP-2�� transcription factor during …

2006 Annual Report, Various Authors

Annual Reports

No abstract provided.

Enhancing In Vitro Degradation Of Alfalfa Hay And Corn Silage Using Feed Enzymes, Jong-Su Eun, K. A. Beauchemin

Animal, Dairy, and Veterinary Science Faculty Publications

A series of in vitro fermentation experiments was performed to assess the effects of 4 feed enzyme products (FE) that varied in enzymatic activities on the degradation of alfalfa hay and corn silage. The FE contained a range of endoglucanase, exoglucanase, xylanase, and protease activities, and a range of dose rates (DR) was used. The objective of the study was to identify effective formulations and optimum DR, and to establish if combining FE would further improve fiber degradation. For alfalfa hay, quadratic increases in gas production and degradation of dry matter (DM) and fiber were observed for all FE, with …