Dairy Science Commons^™

Modeling Severe Fever With Thrombocytopenia Syndrome Virus Infection In Golden Syrian Hamsters: Importance Of Stat2 In Preventing Disease And Effective Treatment With Favipiravir, Brian B. Gowen, Jonna B. Westover, Jinxin Miao, Arnaud J. Van Wettere, Johanna D. Rigas, Brady T. Hickerson, Kie-Hoon Jung, Rong Li, Bettina L. Conrad, Skot Nielson, Yousuke Furuta, Zhongde Wang

Animal, Dairy, and Veterinary Science Faculty Publications

Severe fever with thrombocytopenia syndrome (SFTS) is an emerging tick-borne disease endemic in parts of Asia. The etiologic agent, SFTS virus (SFTSV; family Bunyaviridae, genus Phlebovirus) has caused significant morbidity and mortality in China, South Korea, and Japan, with key features of disease being intense fever, thrombocytopenia, and leukopenia. Case fatality rates are estimated to be in the 30% range, and no antivirals or vaccines are approved for use for treatment and prevention of SFTS. There is evidence that in human cells, SFTSV sequesters STAT proteins in replication complexes, thereby inhibiting type I interferon signaling. Here, we demonstrate …

Identification Of Snps Located On Bta 6 And Bta 20 Significantly Associated With Bovine Respiratory Disease In Crossbred Cattle, S. L. Miller, S. Mizell, R. Walker, T. Page, Matthew D. Garcia

Animal, Dairy, and Veterinary Science Faculty Publications

The objective of the present study was to evaluate single nucleotide polymorphisms (SNPs) located in two quantitative trait locus (QTL) regions (BTA 6 and BTA 20) that are associated with bovine respiratory disease (BRD). A population of 323 crossbred steers sired by five purebred sire breeds during 2010-2013 (Angus, Braford, Braunvieh, Charolais, and Simmental) were evaluated for BRD susceptibility during the finishing process at a commercial feedlot. A total of 21 animals representing all sire breeds were affected with BRD at some time during the finishing process over the 4-year period. Although multiple sire breeds were evaluated in the present …

A Novel Benzodiazepine Compound Inhibits Yellow Fever Virus Infection By Specifically Targeting Ns4b Protein, Fang Guo, Shuo Wu, Justin Julander, Julia Ma, Xuexiang Zhang, John Kulp, Andrea Cuconati, Timothy M. Block, Yanming Du, Ju-Tao Guo, Jinhong Chang

Animal, Dairy, and Veterinary Science Faculty Publications

Although a highly effective vaccine is available, the number of yellow fever cases has increased over the past 2 decades, which highlights the pressing need for antiviral therapeutics. In a high-throughput screening campaign, we identified an acetic acid benzodiazepine (BDAA) compound which potently inhibits yellow fever virus (YFV). Interestingly, while treatment of YFV-infected cultures with 2 MBDAA reduced the virion production by greater than 2 logs, the compound was not active against 21 other viruses from 14 different viral families. Selection and genetic analysis of drug-resistant viruses revealed that replacement of the proline at amino acid 219 (P219) of the …

Large Animal Genetic Engineering Summit 2016 Proceedings, Utah State University

Animal, Dairy, and Veterinary Science Faculty Publications

It is our great pleasure to welcome you to the first Large Animal Genetic Engineering (LAGE) Summit. Our objectives for the summit are twofold: 1) to provide an update on new approaches to the genetic modification of large animal species for development of better animal models for biomedical research, biomanufacturing, and improved agricultural production; and 2) to provide a forum for discussions on funding mechanisms and regulations effecting development of genetically engineered large animals. Our first summit is being held in Bethesda, Maryland, to facilitate participation by representatives from various federal agencies who either fund or regulate the production of …

Complete Genome Sequences Of Three Historically Important, Spatiotemporally Distinct, And Genetically Divergent Strains Of Zika Virus: Mr-766, P6-740, And Prvabc-59, Sangim Yun, Byunghak Song, Jordan C. Frank, Justin G. Julander, Irina A. Polejaeva, Christopher J. Davies, Kenneth L. White, Youngmin Lee

Animal, Dairy, and Veterinary Science Faculty Publications

Here, we report the 10,807-nucleotide-long consensus RNA genome sequences of three spatiotemporally distinct and genetically divergent Zika virus strains, with the functionality of their genomic sequences substantiated by reverse genetics: MR-766 (African lineage, Uganda, 1947), P6-740 (Asian lineage, Malaysia, 1966), and PRVABC-59 (Asian lineage-derived American strain, Puerto Rico, 2015).

Trophoblast Major Histocompatibility Complex Class I Expression Is Associated With Immune-Mediated Rejection Of Bovine Fetuses Produced By Cloning, Heloisa M. Rutigliano, Aaron J. Thomas, Amanda Wilhelm, Benjamin R. Sessions, Brady A. Hicks, Donald H. Schlafer, Kenneth L. White, Christopher J. Davies

Animal, Dairy, and Veterinary Science Faculty Publications

Trophoblast cells from bovine somatic cell nuclear transfer (SCNT) conceptuses express major histocompatibility complex class I (MHC-I) proteins early in gestation, and this may be one cause of the significant first-trimester embryonic mortality observed in these pregnancies. MHC-I homozygous-compatible (n = 9), homozygous-incompatible (n = 8), and heterozygous-incompatible (n = 5) SCNT pregnancies were established. The control group consisted of eight pregnancies produced by artificial insemination. Uterine and placental samples were collected on Day 35 ± 1 of pregnancy, and expression of MHC-I, leukocyte markers, and cytokines were examined by immunohistochemistry. Trophoblast cells from all SCNT pregnancies expressed MHC-I, while …

Prevalence Of Bovine Viral Diarrhea Virus In Bovine Samples From The Intermountain West Of The U.S.A. - Comparison Between Age, Sex, Breed And Diagnostic Methods, David J. Wilson, T. J. Baldwin, E. J. Kelly, A. Van Wettere, G. Hullinger, J. Bunnell

Animal, Dairy, and Veterinary Science Faculty Publications

Prevalence of Bovine viral diarrhea virus (BVDV) (“detected” test results) among all bovine samples tested at the Utah Veterinary Diagnostic Laboratory from 2008 - 2013 was calculated, and results were compared by age, sex, or breed of the cattle and BVDV diagnostic test methods. Necropsies were tested for BVDV when lesions suggestive of infection were identified. Adults, juveniles and most calves were tested by antigen (Ag) capture ELISA, while fetuses and some calves were tested by real-time reverse transcriptase PCR. Cattle originated from Utah and surrounding states. Chi-square analyses were used to test for significant differences in BVDV prevalence between …

Histopathologic Evaluation Of Postmortem Autolytic Changes In Bluegill (Lepomis Macrohirus) And Crappie (Pomoxis Anularis) At Varied Time Intervals And Storage Temperatures, Jami George, Arnaud J. Van Wettere, Blayk B. Michaels, Debbi Crain, Gregory A. Lewbart

Animal, Dairy, and Veterinary Science Faculty Publications

Information is lacking on preserving fish carcasses to minimize postmortem autolysis artifacts when a necropsy cannot be performed immediately. The purpose of this study was to qualitatively identify and score histologic postmortem changes in two species of freshwater fish (bluegill - Lepomis macrochirus; crappie - Pomoxis annularis), at varied time intervals and storage temperatures, to assess the histologic quality of collected samples. A pooled sample of 36 mix sex individuals of healthy bluegill and crappie were euthanized, stored either at room temperature, refrigerated at 4 C, or frozen at 20, and then necropsied at 0, 4, 24, and 48 h …

Feeding A Brown Midrib Corn Silage-Based Diet To Growing Beef Steers Improves Growth Performance And Economic Returns, C. S. Saunders, S. Y. Yang, J.-S. Eun, D. M. Feuz, D. R. Zobell

Animal, Dairy, and Veterinary Science Faculty Publications

A feedlot experiment was performed to determine growth performance, ruminal fermentation characteristics, and economic returns for growing beef steers when fed a brown midrib corn silage-based total mixed ration (BMRT) compared with a conventional corn silage-based total mixed ration (CCST). Twenty-four Angus crossbred steers (initial body weight=258±23.2 kg) in individual pens were used in a completely randomized design (n=12). Intake of dry matter was not different between the treatments. Steers fed the BMRT tended to have greater average daily gain (1.54 vs. 1.42 kg d−1; P=0.09) and gain-to-feed ratio (0.165 vs. 0.146; P=0.07) compared with those fed the CCST. Feeding …

Cytokine Gene Expression In The Maternal-Fetal Interface In Somatic Cell Nuclear Transfer Pregnancies In Small Ruminants, Heloisa M. Rutigliano, Amanda Wilhelm, Justin Hall, Bi Shi, Qinggang Meng, Rusty Stott, Tom D. Bunch, Kenneth L. White, Christopher J. Davies, Irina A. Polejaeva

Animal, Dairy, and Veterinary Science Faculty Publications

The present retrospective study investigates pregnancy rates, incidence of pregnancy losses and large offspring syndrome (LOS), and immune-related gene expression of sheep and goat somatic cell nuclear transfer (SCNT) pregnancies. We hypothesized that significantly higher pregnancy losses observed in sheep SCNT pregnancies compared to goats are due to the increased amounts of T-helper 1 cytokines and pro-inflammatory mediators at the maternal-fetal interface. Sheep and goat SCNT pregnancies were generated using the same procedure. Control pregnancies were established by natural breeding. Although SCNT pregnancy rates at 45 days were similar in both species, pregnancy losses between 45 and 60 days and …

The Broad-Spectrum Antiviral Favipiravir Protects Guinea Pigs From Lethal Lassa Virus Infection Post-Disease Onset, David Safronetz, Kyle Rosenke, Cynthia Martellaro, Heinz Feldmann, Jonna B. Westover, Brian B. Gowen, Atsushi Okumura, Yousuke Furuta, Takashi Komeno, Greg Saturday, Joan Geisbert, Thomas W. Geisbert

Animal, Dairy, and Veterinary Science Faculty Publications

With up to 500,000 infections annually, Lassa virus (LASV), the cause of Lassa fever, is one of the most prevalent etiological agents of viral hemorrhagic fever (VHF) in humans. LASV is endemic in several West African countries with sporadic cases and prolonged outbreaks observed most commonly in Sierra Leone, Liberia, Guinea and Nigeria. Additionally several cases of Lassa fever have been imported into North America, Europe and Asia making LASV a global threat to public health. Despite this, currently no approved therapeutic or vaccine exists to treat or prevent LASV infections. Here, using a passaged strain of LASV that is …

Rift Valley Fever Virus Infection In Golden Syrian Hamsters, Dionna Scharton, Arnaud J. Van Wettere, Kevin W. Bailey, Zachary Vest, Jonna B. Westover, Venkatraman Siddharthan, Brian B. Gowen

Animal, Dairy, and Veterinary Science Faculty Publications

Rift Valley fever virus (RVFV) is a formidable pathogen that causes severe disease and abortion in a variety of livestock species and a range of disease in humans that includes hemorrhagic fever, fulminant hepatitis, encephalitis and blindness. The natural transmission cycle involves mosquito vectors, but exposure can also occur through contact with infected fluids and tissues. The lack of approved antiviral therapies and vaccines for human use underlies the importance of small animal models for proof-of-concept efficacy studies. Several mouse and rat models of RVFV infection have been well characterized and provide useful systems for the study of certain aspects …

Investigating The Filled Gel Model In Cheddar Cheese Through Use Of Sephadex Beads, L. M. Barden, J. A. Osborne, Donald J. Mcmahon, E. A. Foegeding

Nutrition, Dietetics, and Food Sciences Faculty Publications

Cheese can be modeled as a filled gel whereby milkfat globules are dispersed in a casein gel network. We determined the filler effects using Sephadex beads (GE Healthcare Life Sciences, Pittsburgh, PA) as a model filler particle. Ideally, such a model could be used to test novel filler particles to replace milkfat in low-fat cheese. Low-filler (6% particles), reduced-filler (16%), and full-filler (33%) cheeses were produced using either Sephadex beads of varying sizes (20 to 150 μm diameter) or milkfat. Small- and large-strain rheological tests were run on each treatment at 8, 12, and 18 wk after cheese manufacturing. Differences …

Characterization Of Brd4 During Mammalian Post-Meiotic Sperm Development, Jessica M. Bryant, Greg Donahue, Xiaoshi Wang, Mirella Meyer-Ficca, Lacey J. Luense, Angela H. Weller, Marisa S. Bartolomei, Gerd A. Blobel, Ralph G. Meyer, Benjamin A. Garcia, Shelley L. Berger

Animal, Dairy, and Veterinary Science Faculty Publications

During spermiogenesis, the post-meiotic phase of mammalian spermatogenesis, transcription is progressively repressed as nuclei of haploid spermatids are compacted through a dramatic chromatin reorganization involving hyper-acetylation and replacement of most histones with protamines. Although BRDT functions in transcription and histone removal in spermatids, it is unknown whether other BET family proteins play a role. Immunofluorescence of spermatogenic cells revealed BRD4 in a ring around the nuclei of spermatids containing hyper-acetylated histones. The ring lies directly adjacent to the acroplaxome, the cytoskeletal base of the acrosome, previously linked to chromatin reorganization. The BRD4 ring does not form in acrosomal mutant mice. …

Pathology In Practice - Fascioliasis In A Dahl Sheep, David J. Wilson, T. J. Baldwin, Kerry A. Rood

Animal, Dairy, and Veterinary Science Faculty Publications

In early fall 2012, a 48-kg (106-lb) sexually intact male Dahl ram in fair body condition was found dead; the owner observed no clinical signs prior to death. The ram was part of a flock of 50 game animals used for high-fence hunting that was moved among pastures in Idaho, Utah, and Montana. The ram was housed primarily on flood-irrigated pasture in common with approximately 170 captive Rocky Mountain elk (Cervus canadensis). The owner reported that 12 other sheep had died over the past year without observed clinical signs, but none were submitted for postmortem examination. Both sheep and elk …

Dairy Herd - Level Prevalence Of Johne's Disease And Bvd In The Intermountain West Of The U.S.A. And Farm Management Practices And Characteristics For Test-Positive Herds, David J. Wilson, Kerry A. Rood, C. Whitehouse, J. Bunnell, G. M. Goodell, T. M. Byrem

Animal, Dairy, and Veterinary Science Faculty Publications

Herd-level prevalence of Mycobacterium avium subsp. paratuberculosis (MAP), causative agent of Johne’s disease (JD) and Bovine Viral Diarrhea (BVD) virus were estimated on dairy farms in Utah. Duplicate milks were collected at 3-4 day intervals on 5 dates from each bulk tank on participating farms. Samples were tested at separate laboratories for BVD (real-time, RT-PCR) and for JD/MAP (ELISA and qPCR). 151/209 (72%) eligible dairy farms participated. Farms detected positive were: 58 JD (38%) and 14 BVD (9%); 5 farms had both diseases. Follow up visited farms’ (n=22) means, medians: 778,420 milking cows; 20,052 lbs, 20,311 lbs 305d milk; 175,545/ml, …

Efficient Gene Targeting In Golden Syrian Hamsters By The Crispr/Cas9 System, Zhiqiang Fan, Wei Li, Sang R. Lee, Qinggang Meng, Tom D. Bunch, Kenneth L. White, Zhongde Wang, Il-Keun Kong

Animal, Dairy, and Veterinary Science Faculty Publications

The golden Syrian hamster is the model of choice or the only rodent model for studying many human diseases. However, the lack of gene targeting tools in hamsters severely limits their use in biomedical research. Here, we report the first successful application of the CRISPR/Cas9 system to efficiently conduct gene targeting in hamsters. We designed five synthetic single-guide RNAs (sgRNAs)—three for targeting the coding sequences for different functional domains of the hamster STAT2 protein, one for KCNQ1, and one for PPP1R12C—and demonstrated that the CRISPR/Cas9 system is highly efficient in introducing site-specific mutations in hamster somatic cells. We …

2013 Annual Report, Various Authors

Annual Reports

No abstract provided.

Ghrelin Concentrations In Milk And Plasma Of Dairy Cows During Early Lactation, Sameer M. Alhojaily

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Ghrelin is a hormone produced mainly by the cells lining the gastric mucosa. Ghrelin was first extracted from human and rat stomachs, and identified as an endogenous stimulator of growth hormone release. Ghrelin is synthesized and produced in several tissues, but the gastric mucosa remains the major source of circulating ghrelin. Besides growth hormone release, ghrelin stimulates appetite and plays some major roles in different organs. In several studies, ghrelin was described as a hormone with multiple functions and diverse biological actions. Ghrelin exists in two major forms, active ghrelin and inactive ghrelin, and only the active from binds to …

Physiological Impacts And Lactational Performance Of Dairy Cows Fed Brown Midrib Corn Silage During Dry Period Through Early To Midlactation, Alexandra Windley Kelley

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Developing solutions to the metabolic stress experienced by cows during the transition period is very important because it can negatively influence lactational performance. The objectives were to: 1) compare physiological changes through body weight (BW) and concentrations of non-esterified fatty acids (NEFA) and β-hydroxybutyric acid (BHBA) and 2) evaluate feed intake, milk production, and energy balance (EB) of cows fed brown midrib corn silage (BMRCS)-based diets when compared with conventional corn silage (CCS)-based diets during the transition. At 4 wk prior to parturition, 40 dry multiparous Holstein cows …

Halogeton (H. Glomeratus) Poisoning In Cattle: Case Report, Kerry A. Rood, Kip E. Panter, Dale R. Gardner, Bryan L. Stegelmeier, Jeffery O. Hall

Animal, Dairy, and Veterinary Science Faculty Publications

Historically, the most significant losses from halogeton poisoning have been reported in sheep, with multiple catastrophic deaths documented. While recorded death losses in cattle from halogeton poisoning are less common than in sheep, recent cases, including 2 reported here, and anecdotal reports from other ranchers suggest that the impact of halogeton losses in cattle herds in the western United States is much more widespread than originally thought. Halogeton may accumulate up to 30% oxalates; a small amount of the plant (300 g) is enough to cause death in sheep. Oxalates precipitate calcium from the blood, resulting in hypocalcemia, formation of …

Triple Immunoglobulin Gene Knockout Transchromosomic (Tc) Cattle: Bovine Lambda Cluster Deletion And Its Effect On Fully Human Polyclonal Antibody Production, H. Matsushita, A. Sano, H. Wu, J. Jiao, P. Kasinathan, E. J. Sullivan, Zhongde Wang, K. Kuroiwa

Animal, Dairy, and Veterinary Science Faculty Publications

Towards the goal of producing fully human polyclonal antibodies (hpAbs or hIgGs) in transchromosomic (Tc) cattle, we previously reported that Tc cattle carrying a human artificial chromosome (HAC) comprising the entire unrearranged human immunoglobulin (Ig) heavy-chain (hIGH), kappa-chain (hIGK), and lambda-chain (hIGL) germline loci produced physiological levels of hIgGs when both of the bovine immunoglobulin mu heavy-chains, bIGHM and bIGHML1, were homozygously inactivated (bIGHM-/-, bIGHML1-/-; double knockouts or DKO). However, because endogenous bovine immunoglobulin light chain loci are still intact, the light chains are produced both from the hIGK and hIGL genomic loci on the HAC and from the endogenous …

Activating The Expression Of Human K-Rasg12d Stimulates Oncogenic Transformation In Transgenic Goat Fetal Fibroblast Cells, J. Gong, Zhongde Wang, I. Polejaeva, R. Salgia, C. Kao, T. Chen, L. Chen

Animal, Dairy, and Veterinary Science Faculty Publications

Humane use of preclinical large animal cancer models plays a critical role in understanding cancer biology and developing therapeutic treatments. Among the large animal candidates, goats have great potentials as sustainable sources for large animal cancer model development. Goats are easier to handle and cheaper to raise. The genome of the goats has been sequenced recently. It has been known that goats develop skin, adrenal cortex, breast and other types of cancers. Technically, goats are subject to somatic cell nuclear transfer more efficiently and exhibit better viability through the cloning process. Towards the development of a goat cancer model, we …

Johne's Disease, Mycoplasma And Bvd In Utah - Bulk Tank Milk Testing And Comparison To Previous Regional Prevalence And Individual Herd Results Over Time, David J. Wilson, Kerry A. Rood, J. Bunnell, C. Whitehouse, G. M. Goodell, T. M. Byrem

Animal, Dairy, and Veterinary Science Faculty Publications

Dairy herd-level prevalence of Mycobacterium avium subsp. paratuberculosis (MAP), causative agent of Johne’s disease (JD), Mycoplasma spp., and Bovine Viral Diarrhea (BVD) virus were estimated in Utah and surrounding states and compared to previous surveillance results. Milk was collected at 3-4 day intervals on 5 dates (duplicate samples) from each bulk tank on participating farms, samples analyzed separately. One frozen sample was shipped to a laboratory for JD/MAP testing with ELISA and real-time PCR, the other paired sample was shipped to another laboratory for mycoplasma and BVD testing. Mycoplasma was cultured on modified Hayflick medium, standard methods; BVD testing was …

Determination Of The Expression Patterns Of Bovine Non-Classical Major Histocompatibility Complex (Mhc) Class I Proteins, Parveen Parasar

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This project was funded by the United States of Department of Agriculture (USDA), which funds research aimed at improving production and animal health. The aim of this study was to advance knowledge of maternal immune tolerance to the fetus and mechanisms bovine non-classical MHC class I proteins employ to interact with immune cells and render them inert towards the fetus.

A fetus is a tissue graft inside the mother’s uterus yet must be accepted by the mother to maintain a successful pregnancy. Reproductive insufficiency and pregnancy failure are major causes of production loss in cattle, especially in cloned animals. Knowledge …

2012 Annual Report, Various Authors

Annual Reports

No abstract provided.

Regulation And Expression Of Nanog, Oct4, And Sox2 In The Bovine Blastocyst Following Somatic Cell Nuclear Transfer, Justin Scott Hall

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Animal, Dairy, and Veterinary Sciences Department (ADVS) and the Center for Integrated Biosystems (CIB) at Utah State University are studying various molecular mechanisms involved in the animal cloning process. This study involves the extensive network of people, facilities, equipment, and funding already associated with the CIB and ADVS joint project.

Cloning involves many molecular challenges that for the most part have become roadblocks for the normal development of the fetus. The mechanisms necessary to transform an adult cell into a competent stem cell that can then transform and develop into a healthy organism are poorly understood. Some of these …

Analysis Of Single Nucleotide Polymorphism Panels For Bovine Dna Identification, Kimberly A. Blanchard

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Single nucleotide polymorphisms (SNPs) have great power in resolving questions of unknown parentage on ranches or dairies where errors could have been made in pedigree record keeping or when such records were simply not kept. Currently a panel of 88 SNPs based on a panel originally created by USDA-MARC is commercially available from the company Fluidigm^®. Our objective was to determine whether or not the number of SNPs could be reduced to form a smaller, more cost-efficient parentage-testing panel. A smaller panel would be beneficial to farmers and researchers alike in the reduction of time spent running and …

The Micronutrient Profile Of The Typical American Diet Enhances Colorectal Carcinogenesis, Stephany Del Carmen Perez Monsanto

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The typical Western dietary pattern is characterized by the consumption of energy-dense, nutrient-poor foods and has been linked to increased risk of colorectal cancer (CRC). Our research group previously developed the total Western diet (TWD) that emulates typical human dietary intakes of macro- (carbohydrates, proteins, and fats) and micronutrients (vitamins and minerals) on an energy density basis for rodents. In the present study, we sought to determine the impact of TWD on biomarkers of metabolic syndrome and obesity in comparison to a commercial 45% fat diet used for models of diet-induced obesity (DIO diet) and the standard basal AIN93G diet, …

Physiological Level Production Of Antigen-Specific Human Immunoglobulin In Cloned Transchromosomic Cattle, A. Sano, H. Matsushita, H. Wu, J. Jiao, P. Kasinathan, E. J. Sullivan, Z. Wang, Y. Kuroiwa

Animal, Dairy, and Veterinary Science Faculty Publications

Therapeutic human polyclonal antibodies (hpAbs) derived from pooled plasma from human donors are Food and Drug Administration approved biologics used in the treatment of a variety of human diseases. Powered by the natural diversity of immune response, hpAbs are effective in treating diseases caused by complex or quickly-evolving antigens such as viruses. We previously showed that transchromosomic (Tc) cattle carrying a human artificial chromosome (HAC) comprising the entire unrearranged human immunoglobulin heavy-chain (hIGH) and kappa-chain (hIGK) germline loci (named as κHAC) are capable of producing functional hpAbs when both of the bovine immunoglobulin mu heavy-chains, bIGHM and bIGHML1, are homozygously …