Animal Sciences Commons^™

Light Whole Genome Sequence For Snp Discovery Across Domestic Cat Breeds, James C. Mullikin, Nancy F. Hansen, Lei Shen, Heather Ebling, William F. Donahue, Wei Tao, David J. Saranga, Adrianne Brand, Marc J. Rubenfield, Alice C. Young, Pedro Cruz, Carlos Driscoll, Victor David, Samer W. K. Al-Murrani, Mary F. Locniskar, Mitchell S. Abrahamsen, Stephen J. O'Brien, Douglas R. Smith, Jeffrey A. Brockman

Biology Faculty Articles

Background

The domestic cat has offered enormous genomic potential in the veterinary description of over 250 hereditary disease models as well as the occurrence of several deadly feline viruses (feline leukemia virus -- FeLV, feline coronavirus -- FECV, feline immunodeficiency virus - FIV) that are homologues to human scourges (cancer, SARS, and AIDS respectively). However, to realize this bio-medical potential, a high density single nucleotide polymorphism (SNP) map is required in order to accomplish disease and phenotype association discovery.

Description

To remedy this, we generated 3,178,297 paired fosmid-end Sanger sequence reads from seven cats, and combined these data with the …

State Of The River Report For The Lower St. Johns River Basin, Florida: Water Quality, Fisheries, Aquatic Life, And Contaminants 2010, Environmental Protection Board, City Of Jacksonville, University Of North Florida, Jacksonville University

State of the River Report

No abstract provided.

Dopaminergic Input To The Equine Pituitary: Seasonal And Estradiol Effects, Sarah Case Clavier

LSU Master's Theses

Two experiments were conducted to study the effects of season and estradiol administration on dopaminergic input to the equine adenohypophysis. Experiment 1 began in the spring with 8 mares and 8 stallions and was repeated again in the summer, fall, and winter. Horses were given sulpiride injections of eight incremental doses (0.25 to 32 µg/kg BW). Within each group of 8 horses, half received the sulpiride in an increasing manner, the other half in a decreasing manner. Prolactin concentrations peaked in the first 15 to 30 min in all horses in all seasons. Prolactin areas under the curve increased (P < 0.001) with increasing doses of sulpiride, and were highest (P < 0.05) in March for stallions, but in June for mares. The calculated half-maximum values, which should be proportional to the dopaminergic input to the pituitary, were lowest (P < 0.05) in June and greatest in September. The variation in half-maximum values with season indicated a change in dopaminergic input to the pituitary, with lowest input occurring in June in both sexes. Experiment 2 was designed to determine if the degree of dopaminergic input to the adenohypophysis is altered by estradiol administration. Twelve geldings were used. On day 0, geldings received an i.m. injection of either estradiol cypionate (ECP; n = 6) or vegetable oil (n = 6) at one of two dose volumes (2 vs. 10 mL). On day 6, all geldings received an injection of sulpiride at 0.082 µg/kg BW in saline. Sulpiride injections were repeated every other day with the dose increasing each day, from 0.164 to 100 µg/kg BW. Estradiol pretreatment increased (P < 0.05) the prolactin response to sulpiride at the 2.56 µg/kg dose and higher. However, the half-maximum values for ECP-treated and control geldings did not differ, indicating that the amount of sulpiride needed to counterbalance the amount of dopamine reaching the pituitary was unaltered by estradiol treatment. It was concluded that estradiol likely stimulates prolactin production and secretion after sulpiride directly at the lactotrope level, rather than by decreasing hypothalamic dopamine input to the lactotropes.

Effects Of Varying Levels Of Cottonseed Hulls On Growth And Metabolic Indications Of Rumen Development Of Dairy Calves, Ryan Doescher

LSU Master's Theses

A study was conducted to determine the effects of varying levels of cottonseed hulls on growth and metabolic indications of rumen development of dairy calves. Sixty-four Holstein calves (Heifers, n=40; Bulls, n=24) were randomly assigned to one of four dietary treatments which included calf starters containing no cottonseed hulls (control; C), 10% cottonseed hulls (10% CSH), 15% cottonseed hulls (15% CSH), or 20% cottonseed hulls (20% CSH). Calves were fed their respective treatments beginning on day 6 until day 56 of age. Body weights were measured at birth and biweekly thereafter until day 56 of age. Wither and hip heights …

Cell Extract-Based Reprogramming Of Somatic Cells, Laura Whitney Coley

LSU Master's Theses

The differentiation potential of adult stem cells (ASC) has long been thought to be limited to cell lineages present in the organ from which they are derived; however, several studies have challenged this notion by demonstrating that some ASC exhibit a remarkably high degree of plasticity. Unlike terminally differentiated somatic cells, the less differentiated state of ASC can assume the functional phenotypes and expression profiles of cells unique to other tissues. The expansive repertoire of differentiation potential exhibited by ASC suggests these cells possess characteristics similar to pluripotent cells, including epigenomic regulatory pattern. Therefore, ASC may be better equipped for …

Copper Oxide Wire Particles In Feed Pellets For Controlling Gastrointestinal Nematode Infection In Ewes And Lambs, Sarah Tammy Nicole Orlik

LSU Master's Theses

Gastrointestinal nematode parasites cause extensive damage to small ruminants, and Haemonchus contortus is a major concern to production. In the past, small ruminants were dewormed at regular intervals and control methods were based primarily on the use of anthelmintics. At present, anthelmintic resistance has been reported worldwide and has developed into a serious problem for small ruminant management programs. In view of this, alternate control methods are needed. One alternative method is the use of copper oxide wire particles (COWP). Three independent trials were conducted during Spring, Summer, and Fall of 2008. Two trials evaluated the effect of COWP in …

Pregnancy Rates In Beef Cattle Artifically Inseminated With Frozen-Thawed Aged Beef Semen, David Barry Carwell

LSU Master's Theses

The duration of frozen semen storage had no effect on pregnancy rates (60%, 61% and 61%, respectively) in Angus females inseminated with semen from 25 Angus bulls from Time Frames 1 (1960-1975), 2 (1976-1991) and 3 (1992-2006). Pregnancy rates were not different for beef females inseminated with semen from Time Frames 1, 2 and 3 (55%, 62% and 55%, respectively). There were no differences for total and progressive sperm motility across Time Frames 1 (42% and 29%), Time Frame 2 (51% and 38%) and Time Frame 3 (55% and 41%) and percentage of normal and abnormal sperm from bulls in …

Effects Of Serum Addition To Culture Medium On Gene Expression In Day-7 And Day-14 Bovine Embryos, Jaime Manuel Angulo Campos

LSU Master's Theses

The addition of serum to embryo culture media may alter gene expression and trigger development of Large Offspring Syndrome. The objectives of this study were to determine gene expression levels in embryos cultured in the absence or presence of 5% calf serum and compare these expression patterns to in vivo derived embryos (IVD), and to determine the effects of serum on the length of day-14 embryos. Abattoir derived oocytes were fertilized and cultured in mSOFaa. At 72 hours post-insemination (hpi), embryos were randomly allocated into two treatments: mSOFaa without and with 5% calf serum. Embryos were then cultured to 168 …

Effects Of Meal Timing On Anabolic Hormone Status And Energy Metabolism In Neonatal Dairy Calves, Katherine Claire Simon

LSU Master's Theses

Twelve neonatal Holstein bull calves (38.52 ± 5.87 kg) were fed milk replacer at a fixed or varied meal time to determine the effects on metabolic hormone secretion, average daily intake, growth, and energy metabolism. Body weights were measured every two weeks from birth to 8 weeks. Rumen fluid was collected every two weeks from week 2 through 8. Serial blood collections were conducted every two weeks from week 2 through 8. Blood was collected, beginning at 0530, at 0, 15, 30, 45, 60, 75, 90, 105, 120, 135, and 150 minutes. Plasma was analyzed for ghrelin, leptin, growth hormone …

Laser-Assisted Zona Pellucida Hatching Of Frozen-Thawed In Vivo-Produced Bovine Embryos, Mindy Kaye Chiasson

LSU Master's Theses

Incomplete zona hatching or failure of the zona to rupture compromises post-transfer embryo viability and conceptus development. Assisted hatching prior to the transfer of frozen-thawed bovine embryos has been proposed as a means to increase recipient pregnancy rates. The objective of this study was to determine if laser-assisted hatching would improve in vivo-produced frozen-thawed bovine embryo hatching and pregnancy rates. In Experiment I,II and III frozen direct-transfer embryos received either two a or three symmetrical rents at either 40% or 80% through the outer zona surface using the Hamilton Thorne XYClone® (Hamilton Thorne Biosciences) diode laser at 90% power with …

The Interictal State In Epilepsy And Behavior, Daniel Tice Barkmeier

Wayne State University Dissertations

Epilepsy is one of the most common neurological diseases, affecting up to 1% of the world population. Epilepsy remains poorly understood and there are currently no medications to cure it. Patients with epilepsy have both seizures as well as another type of abnormal activity between seizures, known as interictal spikes. Interictal spikes have thus far been poorly researched, yet growing evidence supports an important role for them in epilepsy. In this project, we first show the high variability between reviewers in marking interictal spikes on intracranial EEG, and then develop and test an automated detection method to solve this problem. …

Promiscuous Mating In Feral Pigs (Sus Scrofa) From Texas, Usa, Johanna Delgado-Acevedo, Angeline Zamorano, Randy W. Deyoung, Tyler A. Campbell, David G. Hewitt, David B. Long

United States Department of Agriculture Wildlife Services: Staff Publications

Context. Feral pigs represent a significant threat to agriculture and ecosystems and are disease reservoirs for pathogens affecting humans, livestock and other wildlife. Information on the behavioural ecology of feral pigs might increase the efficiency and effectiveness of management strategies.

Aims. We assessed the frequency of promiscuous mating in relation to oestrous synchrony in feral pigs from southern Texas, USA, an agroecosystem with a widespread and well established population of feral pigs. An association between multiple paternity of single litters and synchrony of oestrous may indicate alternative mating strategies, such as mateguarding.

Methods. We collected gravid sows at …

Shorebird Migration In Western Kentucky: Phenology, Habitat Use, And Possible Effects Of Prey Availability, Nicole Ann Ranalli

Online Theses and Dissertations

Staging areas along the coasts provide reliable food resources and shorebirds may use the same stopover locations every year. However, shorebirds use sites opportunistically in the interior of North America due to the transient nature of many habitats. Little is known, however, about the use of wetlands by migrating shorebirds in Kentucky. During 2004 and 2005, I examined the phenology of migration and habitat use of shorebirds using stopover habitats in Kentucky, and also examined possible relationships between prey availability and habitat selection by migrating shorebirds. To mitigate wetland loss, the Kentucky Department of Fish and Wildlife Resources (KDFWR) constructed …

Department Of Fisheries Annual Report To The Parliament 2009/10, Government Of Western Australian Department Of Fisheries

Department of Fisheries Annual Reports

This annual report provides an overview of the Department and its operations for the financial year ended 30 June 2010. It has been prepared in accordance with the requirements of the Financial Management Act 2006, the Fish Resources Management Act 1994 and the Public Sector Commission’s Annual reporting framework – 2009-10 reporting year.

Migration Of Northern Yellowstone Elk: Implications Of Spatial Structuring, P. J. White, Kelly M. Proffitt, L. David Mech, Shaney B. Evans, Julie A. Cunningham, Kenneth L. Hamlin

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Migration can enhance survival and recruitment of mammals by increasing access to higher-quality forage or reducing predation risk, or both. We used telemetry locations collected from 140 adult female elk during 2000– 2003 and 2007–2008 to identify factors influencing the migration of northern Yellowstone elk. Elk wintered in 2 semidistinct herd segments and migrated 10–140 km to at least 12 summer areas in Yellowstone National Park (YNP) and nearby areas of Montana. Spring migrations were delayed after winters with increased snow pack, with earlier migration in years with earlier vegetation green-up. Elk wintering at lower elevations outside YNP migrated an …

Quantitative Pcr Assay For Mycobacterium Pseudoshottsii And Mycobacterium Shottsii And Application To Environmental Samples And Fishes From The Chesapeake Bay, D. T. Gauthier, K. S. Reece, J. Xiao, M. W. Rhodes, H. I. Kator, R. J. Latour, C. F. Bonzek, J. M. Hoenig, W. K. Vogelbein

Biological Sciences Faculty Publications

Striped bass (Morone saxatilis) in the Chesapeake Bay are currently experiencing a very high prevalence of mycobacteriosis associated with newly described Mycobacterium species, Mycobacterium pseudoshottsii and M. shottsii. The ecology of these mycobacteria outside the striped bass host is currently unknown. In this work, we developed quantitative real-time PCR assays for M. pseudoshottsii and M. shottsii and applied these assays to DNA extracts from Chesapeake Bay water and sediment samples, as well as to tissues from two dominant prey of striped bass, Atlantic menhaden (Brevoortia tyrannus) and bay anchovy (Anchoa mitchilli). Mycobacterium …

Morphological Differences Among Eyeless Amphipods In The Genus Stygobromus Dwelling In Different Subterranean Habitats, David C. Culver, John R. Holsinger, Mary C. Christman, Tanja Pipan

Biological Sciences Faculty Publications

The amphipod genus Stygobromus occurs in a variety of subterranean habitats in North America, including caves, phreatic (groundwater) lakes, and superficial subterranean habitats (seeps and epikarst). The habitats share the absence of light but differ in other features, such as pore size of the habitat, available food, and degree of seasonality. Measurements of body size, antennal size, and antennal segment number of type specimens were compared for 56 species occurring in the eastern United States. Except for differences in body size, differences among species in the four different habitats were not significant. Body size was related to relative pore size …

Spawning-Related Movements Of Barred Sand Bass Paralabrax Nebulifer, In Southern California: Interpretations From Two Decades Of Historical Tag And Recapture Data, E. T. Jarvis, Christi Linardich, C. F. Valle

Biological Sciences Faculty Publications

During the 1960s and 1990s, the California Department of Fish and Game tagged 8,634 barred sand bass in southern California, and 972 fish (11%) were recaptured. Tag returns suggest barred sand bass are transient aggregate spawners that form spawning aggregations consisting of both resident and migrant individuals. Spawning residency at a historic spawning location was estimated by the frequency of returns over time; most same-year returns (82%, n  =  141) were recaptured within a 7 to 35-day period. The maximum recapture distance was 92 km. The average (± SD) non-spawning season recapture distance from peak spawning season tagging locations was …

Conservation Status And Habitat Use Of The West Virginia Spring Salamander (Gyrinophilus Subterraneus) And Spring Salamander (G. Porphyriticus) In General Davis Cave, Greenbrier Co., West Virginia, Matthew L. Niemiller, Michael S. Osbourn, Dante B. Fenolio, Thomas K. Pauley, Brian T. Miller, John R. Holsinger

Biological Sciences Faculty Publications

The West Virginia Spring Salamander (Gyrinophilus subterraneus) is one of four obligate cave-dwelling species of plethodontid salamanders found east of the Mississippi River in the United States. This species is endemic to a single cave system; General Davis Cave, in Greenbrier Co., West Virginia, where it is syntopic with the closely-related Spring Salamander (G. porphyriticus). Accordingly, the West Virginia Spring Salamander is a species of critical conservation concern. Because of it's conservation status and lack of data regarding the ecology and life history, particularly about population trends, we present data on relative abundance of and habitat …

In The News, Joe N. Caudell

Human–Wildlife Interactions

No abstract provided.

The Soap Box Wild Horses And Blm Management Issues: What To Do With 30,000 Symbols Of The American West, James T. Smith

Human–Wildlife Interactions

No abstract provided.

Relative Habitat- And Browse-Use Of Native Desert Mule Deer And Exotic Oryx In The Greater San Andres Mountains, New Mexico, Brock D. Hoenes, Louis C. Bender

Human–Wildlife Interactions

Introduced oryx (Oryx gazella gazella) have expanded into the San Andres Mountains of south-central New Mexico, but little is known of concurrent habitat used by oryx and desert mule deer (Odocoileus hemionus crooki); the latter in New Mexico is a species of special concern that has declined significantly since the introduction of oryx. We used fecal-pellet and browse surveys in combination with presence modeling to identify differences in relative use of habitat types, distribution, and browsing of highly palatable, highly preferred (hereafter, key) plant species during 2004 to 2006 to assess the potential for direct competition …

Estimating Relative Distribution Of Raccoons, Opossums, Skunks, And Foxes Using Animal Control Data, Christine Klinkowski-Clark, Michael J. Kutilek, John O. Matson, Paula Messina, Kevin Earley, Shannon M. Bros-Seemann

Human–Wildlife Interactions

We used indices of animal control reports per capita and areas of land covers to assess the relative habitat-use of raccoons (Procyon lotor), opossums (Didelphis virginiana), skunks (Spilogale putorius and Mephitis mephitis), and foxes (Vulpes vulpes and Urocyon cinereoargenteus). We used confirmed (hereafter, verified) calls made to Brevard Animal Services, Florida, and assessed potential human demographic influences associated with unconfirmed (hereafter, unverified) reports where it was uncertain whether or not an animal was present. To estimate habitat use, we performed quadrat sampling using a geographic information system (GIS) and obtained areas of …

Landowner Attitudes Toward Elk Management In The Pine Ridge Region Of North-Western Nebraska, R. Daniel Crank, Scott E. Hygnstrom, Kit M. Hams

Human–Wildlife Interactions

Little is known about attitudes of landowners toward elk (Cervus elaphus) on privately-owned land. We mailed questionnaires to agricultural landowners in the Pine Ridge region of northwestern Nebraska in both 1995 and 1997 to determine attitudes toward elk populations and management of elk. Fifty-six percent (n = 214) of respondents in 1995 and 57% (n = 461) in 1997 were in favor of free-ranging elk. Motivation for those in favor of elk was utilitarian (opportunity to view and hunt elk), ecological (return of a native species), and economic (benefits from increased tourism and leased land for elk hunting). …

Effects Of Aquaculture On Migration And Movement Patterns Of Double-Crested Cormorants, D. Tommy King, Bradley F. Blackwell, Brian S. Dorr, Jerrold L. Belant

Human–Wildlife Interactions

We analyzed 10,620 recovery records for double-crested cormorants (Phalacrocorax auritus) banded as nestlings from 1923 to 2006 to determine the population’s age structure, migration routes, dispersal patterns, and the possible influence of the expansion of the aquaculture industry in the southeastern United States on these population characteristics. Ninety-nine percent of the birds were banded during June to August, and 78% were banded as pre-fledged birds. Cormorants banded in the interior region of the United States comprised 91% of all birds banded from 1955 to 2006; these birds wintered primarily in the Lower Mississippi Valley and the northern Gulf …

Testing Fladry As A Nonlethal Management Tool For Wolves And Coyotes In Michigan, Sarah J. Davidson-Nelson, Thomas M. Gehring

Human–Wildlife Interactions

Several forms of nonlethal management exist, but fi eld testing is problematic, and few such techniques have been tested on free-ranging wolves (Canis lupus) or other predators. We tested fladry in the eastern Upper Peninsula of Michigan during the summers of 2004 and 2005 on treatment farms and control farms. Wolf visitation inside pastures, compared to those outside pastures, was less on fladry-protected farms (U = 45, n = 7, P = 0.004); whereas, we found no difference in wolf visitation inside and outside of pastures on control farms (U = 30, n = 7, P …

Habitat Correlates Of Jaguar Kill-Sites Of Cattle In Northeastern Sonora, Mexico, Octavio C. Rosas-Rosas, Louis C. Bender, Raul Valdez

Human–Wildlife Interactions

Predation on cattle by the endangered jaguar (Panthera onca) can be a serious ecological and economic conflict. We investigated habitat characteristics of kill sites of cattle in Sonora, Mexico, from 1999 to 2004 to see whether habitat management or cattle distribution could be used as effective nonlethal methods to limit predation. Kill-sites were positively associated with oak, semitropical thornscrub, and xeric thornscrub vegetation types, whereas they were negatively associated with upland mesquite. Sites of cattle kills were also positively associated with proximity to permanent water sources and roads. A model including these relationships fi t kill locations well …

Commentary Euthanasia Methods In Field Settings For Wildlife Damage Management, Timothy J. Julien, Stephen M. Vantassel, Scott R. Groepper, Scott E. Hygnstrom

Human–Wildlife Interactions

No abstract provided.

Movements, Habitat Selection, Associations, And Survival Of Giant Canada Goose Broods In Central Tennessee, Eric M. Dunton, Daniel L. Combs

Human–Wildlife Interactions

The brood-rearing period in giant Canada geese (Branta canadensis maxima) is one of the least-studied areas of goose ecology. We monitored 32 broods in Putnam County, Tennessee, from the time of hatching through fledging (i.e., when the goslings gained the ability to fly) and from fledging until broods left the brood-rearing areas during the spring and summer of 2003. We conducted a fixed-kernel, home-range analysis for each brood using the Animal Movement Extension in ArcView® 3.3 GIS (ESRI, Redlands, Calif.) software and calculated 95% and 50% utilization distributions (UD) for each brood. We classified 25 broods as sedentary …

Nest-Site Selection And Nesting Ecology Of Giant Canada Geese In Central Tennessee, Jason S. Carbaugh, Daniel L. Combs, Eric M. Dunton

Human–Wildlife Interactions

Little information is available on giant Canada goose (Branta canadensis maxima) nest-site selection on isolated nesting ponds. We monitored 46 island and 72 shoreline nests in the Upper Cumberland (UC) region of central Tennessee during 2002 and 2003. We measured 6 habitat variables at nesting ponds and randomly-selected non-nesting ponds. We used logistic regression to determine which habitat variables were important in nest-site selection. Presence of an island was the most important variable, but it was excluded from the final analysis because of quasi-separation (i.e., geese nested on all known islands in the study area). Geese that nested …