Life Sciences Commons^™

Monitoring Stem Cell Differentiation Using Raman Microspectroscopy: Chondrogenic Differentiation, Towards Cartilage Formation, Francesca Ravera, Esen Efeoglu, Hugh Byrne

Articles

Mesenchymal Stem Cells (MSCs) have the ability to differentiate into chondrocytes, the only cellular components of cartilage and are therefore ideal candidates for cartilage and tissue repair technologies. Chondrocytes are surrounded by cartilage-like extracellular matrix (ECM), a complex network rich in glycosaminoglycans, proteoglycans, and collagen, which, together with a multitude of intracellular signalling molecules, trigger the chondrogenesis and allow the chondroprogenitor to acquire the spherical morphology of the chondrocytes. However, although the mechanisms of the differentiation of MSCs have been extensively explored, it has been difficult to provide a holistic picture of the process, in situ. Raman Micro Spectroscopy (RMS) …

Sure 2021 Undergraduate Science Conference Booklet, Sure Network

Group Reports

The SURE 2021 Conference was the fourth series of Science Undergraduate Research Experience (SURE) Conferences and second online conference, following earlier face to face series in 2018 (with three conferences in Dublin, Athlone and Waterford) and in 2019 (with three conferences in Dublin, Sligo and Carlow). The 2021 virtual online conference was hosted by Waterford Institute of Technology (WIT), TUS:MM (Athlone Institute of Technology) and Dundalk Institute of Technology (Dundalk IT) and featured 23 oral presentations and 48 poster presentations across three thematic streams/break out rooms on zoom. An interactive virtual poster session took place using …

Ankle-Knee Initial Contact Angle And Latency To Maximum Angle Are Affected By Prolonged Run, Sydni Wilhoite, Jessica Mutchler, Barry Munkasy, Li Li

Health Sciences & Kinesiology: Faculty Publications

The initial contact and midstance angles may influence injury risk. Previous literature has not assessed these angles under the influence of new footwear for a non-exhaustive prolonged run or the relationship between the angles. To assess lower extremity kinematic changes and the relationship between kinematic parameters at initial contact and midstance with prolonged running under the influence of different types of footwear. Twelve experienced, recreational runners (6 male; 6 female; 24.8 ± 8.4 years; 70.5 ± 9.3 kg; 174.1 ± 9.7 cm) ran for 31 minutes at a self-selected pace for three testing sessions wearing maximalist, habitual, and minimalist shoes. …

Campus Recreation And Fitness Center Utilization During The Covid-19 Pandemic, Keagan Kiely, William Mase, Bridget F. Melton, Haresh Rochani Dr.

Health Sciences & Kinesiology: Faculty Publications

Background

The impact of the COVID-19 pandemic has far reaching effects for college students' health and physical activity behaviors. This analysis focuses on university student fitness center usage pre and during the COVID-19 pandemic. Researchers hypothesized a reduction in fitness center utilization when comparing Fall 2019 to Fall 2020 utilization rates.

Methods

Patterns of the recreation center and fitness center utilization during the COVID-19 pandemic are compared to pre-pandemic patterns of a matched time period in the previous academic year in an observational study. Overall utilization was evaluated using secondary data from the university’s recreation center data system. Six weeks …

Telehealth Optimization – Role Of Clinical Athletic Trainers In Orthopedic/Sports Medicine Practice, Katie Harbacheck, Laura Grambo, Shaun Keefer, Eric Klein, Christine Boyd, Carolyn Ford-Hemann, Cameron D’Alpe, Steve Frick, Kevin Shea

Clinical Practice in Athletic Training

Background: Prior to COVID-19, telehealth (TH) implementation in most health systems was minimal. The transition to high rates of TH was dramatic in response to COVID-19, and many health systems struggled to develop optimal, efficient TH workflows. Hypothesis/Purpose: The primary purpose of this study was to evaluate the role of clinically integrated athletic trainers (ATs) into the TH transition of a major orthopedic academic center through growth of TH visits as well as physician satisfaction. Methods: Workflows and tip sheets were designed to include considerations of TH visit increases, adoption of appropriate technology platforms and optimal staff models. The rapid …

Stratifying Ischaemic Stroke Patients Across 3 Treatment Windows Using T2 Relaxation Times, Ordinal Regression And Cumulative Probabilities, Bryony Mcgarry, Elizabeth Hunter, Robin Damian, Michael Knight, Philip Clatworthy, George Harston, Keith Muir, Risto Kauppinen, John Kelleher

Conference papers

Unknown onset time is a common contraindication for anti-thrombolytic treatment of ischaemic stroke. T2 relaxation-based signal changes within the lesion can identify patients within or beyond the 4.5-hour intravenous thrombolysis treatment-window. However, now that intra-arterial thrombolysis is recommended between 4.5 and 6 hours from symptom onset and mechanical thrombectomy is considered safe between 6 and 24 hours, there are three treatment-windows to consider. Here we show a cumulative ordinal regression model, incorporating the T2 relaxation time, predicts the probabilities of a patient being within one of the three treatment-windows and is more accurate than signal intensity changes from T2 weighted …

Parents' Attitudes And Beliefs Towards Dietary Behaviors Of Children With Autism Aged 5-13 Years, Subashree Narayanan

UNF Graduate Theses and Dissertations

Autism is a neurodevelopmental disability that may affect nutritional management of children with autism. Parent’s attitudes and their behavior towards healthy eating reflects on the child’s eating habits and thus, their nutritional health. This study aimed to describe the diet quality of children with autism using the Healthy Eating Index (HEI) scores. It also aimed to assess the relationship between parent’s perceived barriers and self-efficacy and children’s dietary behavior and dietary intake (age 5-13 years). The relationship was assessed using 2 questionnaires, Nutrition and Health Awareness and BAMBI questionnaire for parent’s attitudes and beliefs and dietary behavior, respectively. Dietary intake …

Therapeutic Potential Of Mitophagy-Inducing Microflora Metabolite, Urolithin A For Alzheimer’S Disease, Dona Pamoda W. Jayatunga, Eugene Hone, Harjot Khaira, Taciana Lunelli, Harjinder Singh, Gilles J. Guillemin, Binosha Fernando, Manohar L. Garg, Giuseppe Verdile, Ralph N. Martins

Research outputs 2014 to 2021

Mitochondrial dysfunction including deficits of mitophagy is seen in aging and neuro-degenerative disorders including Alzheimer’s disease (AD). Apart from traditionally targeting amyloid beta (Aβ), the main culprit in AD brains, other approaches include investigating impaired mitochondrial pathways for potential therapeutic benefits against AD. Thus, a future therapy for AD may focus on novel candidates that enhance optimal mitochondrial integrity and turnover. Bi-oactive food components, known as nutraceuticals, may serve as such agents to combat AD. Uro-lithin A is an intestinal microbe-derived metabolite of a class of polyphenols, ellagitannins (ETs). Urolithin A is known to exert many health benefits. Its antioxidant, …

2021 Weed Science Research Report – Gold Section, Ndsu Weed Science, Dept. Of Plant Sciences

Plant Sciences Weed Science Reports

The Weed Science team at North Dakota State University conduct field research in the State of North Dakota. This specific document reports on field research conducted in 2021. It includes work on sugarbeets and the management of several weeds in North Dakota.

Native Seed Scoop, Winter 2021, University Of Northern Iowa. Tallgrass Prairie Center.

Native Seed Scoop

Contents:

--- Fourth Public Meeting - An Assessment of Native Seed Needs and Capacities
--- Native Seed Stakeholder Meeting(s) 2021
--- Native Seed News and Info
--- Production Topic - Seed Cleaning
--- Species Spotlight
--- Other news . . .

A Review Of The Molecular Mediators Of Host-Baculovirus Interactions In Lepidoptera, Olivia Crouse

Honors Program Theses

Iowa farmlands occupy approximately 85% of its 35.7 million acres, and Iowa leads the United States in corn and soybean production, with an estimated 2.58 billion and 502 million bushels of corn and soybean produced in 2019, respectively. This successful trend in annual yields has span forty plus years and been consequential to the simultaneous implementation of agricultural- and science-based practices. Genetically modified corn and soybean crop varieties express genes from Bacillus thuringiensis (Bt) which encode insecticidal proteins that protect, with high specificity, against insect pests. While researchers continue to study the molecular mechanism(s) of Bt extensively, alternative environmentally safe …

Bacillus Bacteriophage Q11 May Have A 5' Covalently Attached Protein, Emily Cornelius

Honors Program Theses

Bacteriophages (phages) are viruses that specifically parasitize on bacteria and are found almost everywhere. Phages Phi 29 and Q11 are both small phages found in soil that attack Bacillus hosts. Previous Phi 29 studies verified a covalently bonded protein at the 5’ end of its double-stranded linear DNA. Q11 may have a similar 5’ terminal protein, which blocks complete sequencing of that DNA region. Because Q11 and phi29 are both small short-tailed phages that infect similar hosts, it could be possible the proteins are structurally similar. In previous work, I have attempted to use restriction endonuclease and exonuclease enzyme digests …

The Effects Of Fluoride On Macrophage Thp-1 Cell Apoptosis, Jessica Heims

Honors Program Theses

Fluoride is an earthly mineral that occurs naturally both in the sedimentary earth as well as in the human body. The main biological function of fluoride in the human body is to enhance bone structure, as well as prevent the decay of teeth. This mineral has been used by dentists and public health officials to artificially introduce an extra layer of protection through the means of fluoride-enriched public water supplies and toothpastes. By using extra levels of fluoride, dentists have been able to help patients prevent future cavities and dental diseases. Although most dentists highly encourage strategic consumption of these …

Evaluation Of Archeal Growth On Complex Media And Antibiotic Regimes, Melanie Miller

Honors Program Theses

The first Archaea that were studied by scientists came from extreme environments such as fissures at the bottom of oceans where hydrothermal vents formed. It was perhaps surprising that later sampling of environmental DNA revealed Archaea inhabit normal environments as well, for example they are globally distributed in soils. These mesophilic soil species were only recently grown in laboratory pure cultures. In this study, we will expand on this work by feeding the community of soil microbes with various dilutions of tryptic soy broth and other complex media in combination with an antibiotic regime. This will allow us to compare …

Examining The Effects Of Netropsin On The Mobility Of Dna A-Tracts Using Capillary Electrophoresis, Jillian Miller

Honors Program Theses

A-tracts are sequences of repeated adenine bases that cause a natural curvature of DNA. The biological function of A-tracts is not fully understood. Research into how A-tract sequences affect mobility in an electric field contributes more knowledge in this area. For this research project, the electrophoretic mobility of multiple double-stranded DNA (dsDNA) fragments of the same length, with varying shapes due to the amount and orientation of A-tracts along the helix axis, was studied using capillary electrophoresis under varying conditions with the small molecule netropsin. Previous semesters of research showed a change in mobility due to shape of three fragments …

Optimizing Dna Extraction From Microorganisms Living In Wind Cave, Allison Warming

Honors Program Theses

Wind Cave National Park in South Dakota is home to many creatures, from the giant buffalo to microscopic single-celled organism. This study focused on the single-celled organisms living in Wind Cave. A total of 81 samples were collected and grown on culture media. The goal of this project was to determine which commercially available kit would yield the highest amount of purified DNA from the samples cultured from the cave. Following the protocols provided by the manufacturer, a control sample as well as samples from three plates had their DNA isolated and purified using seven commercially available DNA purification kits. …

Landscape Genetics Of Iowa's Threatened Black Redhorse, Morganne C. Borsh

Honors Program Theses

The objectives of this study are to:

a. Characterize the genetic diversity of populations of Black Redhorse in Iowa

b. Determine the level of connectivity between populations in Iowa

Overall, this project aims to determine the degree of fragmentation of Black Redhorse populations to better direct conservation strategies. This study utilizes genome-wide Single Nucleotide Polymorphisms (SNPs) to assess the population genetic structure of the Black Redhorse across its range in northeast Iowa.

The Search For Life In Extreme Environments: Measuring Diversity And Quantity Of Archaea In Wind Cave National Park, Nicole Geerdes

Honors Program Theses

Archaea have the potential to play an essential role in their environment at the base of the food chain. In particular, most of the known species in the phylum Thaumarchaea are autotrophs that can fix their own carbon using energy from ammonia oxidation, that is, they produce their own organic molecules from carbon dioxide by burning nitrogen-based fertilizer. Since their discovery, it has been difficult to learn more about these microbes because they do not grow well in pure laboratory culture. Therefore, much of what is known about them comes from analyzing environmental DNA samples. This allowed researchers to discover …

A Descriptive Analysis Of Intuitive Eating On The Uni Campus, Natalee Jeanne Lyons

Honors Program Theses

Over the course of this project, we will send out a cross-sectional survey to undergraduate students living on UNI’s campus. Included in the survey are questions regarding nutritional campus climate, student’s food preferences and eating habits, as well as the complete second edition of the Intuitive Eating Scale (Tylka & Kroon Van Diest, 2013).

This survey will then be analyzed utilizing SPSS software to draw conclusions regarding this target population (IBM Corp, 2016). Upon completion of this data analysis, our research 2 team hopes to address the following questions: What factors influence the eating behaviors of college students living on …

Identification Of Fungi And Their Secondary Metabolites From Wind Cave, Kyle Biscoglia

Honors Program Theses

Eighteen fungal samples were isolated from samples of water collected in Wind Cave, South Dakota. Ten of the samples underwent chemical extraction, a growth inhibition test, and were analyzed using proton nuclear magnetic resonance spectroscopy and liquid chromatography-mass spectroscopy. Structural analysis and identification of one secondary metabolite that demonstrated antimicrobial growth inhibition was performed. All eighteen of the fungal samples were able to be identified to the genus level using the ITS region of their DNA, however confident identification of the species level was more difficult to attain just looking at one portion of the sequence.

Consultation Summary For Proposed Declared Pest Rates 2021/2022, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity published reports

In accordance with the Biosecurity and Agriculture Management (Declared Pest Account) Regulations 2014, the Minister for Agriculture and Food carries out consultation on Declared Pest Rates, to be considered prior to the rates being determined and gazetted.

This document is the Department of Primary Industries and Regional Development's response to the submissions received.

Pericardial Fluid Proteomic Label-Free Quantification Of Differentially Expressed Proteins In Ischemic Heart Disease Patients With Systolic Dysfunction By Nano-Lc-Esi-Ms/Ms Analysis, Junaid Ullah, Satwat Hashmi, Arslan Ali, Faisal Khan, Shahid Ahmed Sami, Nageeb Basir, Syedah Saira Bokhari, Hasanat Sharif, Hesham R. El-Seedi, Syed Ghulam Musharraf

Department of Biological & Biomedical Sciences

Left ventricular systolic dysfunction (LVSD) is common in patients with pre-existing ischemic heart disease (IHD) and myocardial infarction. An untargeted proteomic approach is used to improve the understanding of the molecular mechanisms associated with LVSD and to find out potential proteomic signatures in pericardial fluid. The pericardial fluid of IHD (n = 45) patients was grouped into two categories according to the left ventricular ejection fraction, LVEF ≥45 (n = 33) and LVEF <45 (n = 12), and analyzed by using nano-liquid chromatography–mass spectrometry (nano-LC-MS/MS) technique. The nano-LC-MS/MS analysis resulted in the identification of 709 pericardial fluid (PF) proteins …

Changes In The Vertical Stratification Of Neotropical Nymphalidae At Forest Edges In Relation To Light And Temperature Conditions, Brian Koji Oye

University of the Pacific Theses and Dissertations

The composition of neotropical fruit-feeding nymphalid butterflies assemblages often varies by location and stratum within a forest. Recent work has shown that vertical stratification in particular may serve as barrier to gene flow, indicating the potential role vertical stratification may play in evolution. At forest edges, the distinction between assemblages has been described to decrease, with species considered to be canopy specialists descending to the forest understory. The similarity in light conditions between the canopy and understory strata at edges or disturbed habitat is hypothesized to be responsible for this phenomenon. We conducted a study using standardized sampling to document …

Influences On Conifer Drought Responses In Northern California, Wallis L. Robinson

Cal Poly Humboldt theses and projects

California is experiencing increasingly severe and prolonged droughts, which are contributing to changes in tree stress and forest mortality. Many factors affect a tree’s drought response, including competition, climate, and site and tree characteristics. Northern California provides a suitable venue to explore the effects of these factors, as it spans a variety of site conditions and includes habitat for conifers with different adaptations and requirements. This study used annual ¹³C discrimination and growth metrics to assess differences in drought resistance and resilience in conifers adapted to coastal and montane ranges at both wet and dry sites, as well as …

The Effects Of Turn Amplitude And Spatial Frequency On The Energetic Cost Of Running, Adam B. Grimmitt

Cal Poly Humboldt theses and projects

Compared to road running, those who run off-road face many unique challenges that impact their running mechanics and energetics. Current literature fails to address how the turns encountered on off-road paths might influence running performance. Therefore, we aimed to investigate the effects of turn angle and frequency on the energetic cost of running. Twelve young adult runners (21±3 years) who run ≥ 30 miles/week and who can run < 22 min for 5 kilometers participated in this study. All subject performed four trials: one straight path trial and three turning paths trials with combined spatial frequencies (ξ- cycles per meter) and amplitudes (meters) of 0.083SF:4AMP, 0.167SF:4AMP, and 0.167SF:2AMP, respectively. Using breath by breath portable gas analysis, we measured V̇O2 and V̇CO2 in the last 2 minutes of each 6-minute trial to determine metabolic power (Watt/kg). Statistical analysis utilized a repeated measures analysis of variance (ANOVA) and a post-hoc paired-sampled T-test to evaluate differences in energetic costs between paths. Compared to a virtually straight path, runners on average consumed 14% and 27% more metabolic energy on a path with turns of 0.167:2 (p=.002) and 0.167:4 (p

Effects Of Large Wood Restoration On Coho Salmon In A Northern California Watershed: A Before-After-Control-Impact Experiment, Natalie B. Okun

Cal Poly Humboldt theses and projects

Substantial time, money, and effort are invested in river and stream restoration projects to aid in the recovery of imperiled salmonid populations, but there is little evidence that these efforts have had lasting positive impacts on juvenile fish growth and survival. To assess the effectiveness of large woody debris (LWD) restoration, which is one of the most common restoration practices, I evaluated the growth and survival response of endangered Central California Coast coho salmon (Oncorhynchus kisutch) in a paired watershed before-after impact-control (BACI) study. To determine if LWD supplementation influenced coho salmon growth and survival, two neighboring, similar …

Effects Of A Dual-Task Paradigm On Tandem Gait Performance, Courtney Angela Perry

Cal Poly Humboldt theses and projects

In concussion management, a variety of cognitive and motor control tests, like the tandem gait, have been used for assessing the presence of a concussion injury at the sideline and through recovery. More recently, research suggests that introducing a secondary cognitive retention task during tandem gait (i.e., dual task) may provide a better assessment of concussion. The purpose of this study was to determine if a tandem gait test paired with a 6-digit retention is a valid and reliable tool. Participants completed three trials of both the single-task (ST) and dual-task (DT) tandem gait tests, and their average (MEAN) and …

Evaluating The Effects Of Residual Broiler Litter And Copper In Soil On Hemp (Cannabis Sativa L.) Fertility And Cannabinoid Production, Sarah E. Forden

Murray State Theses and Dissertations

This study encases two separate experiments observing hemp (Cannabis sativa L.) and soil fertility with the use of broiler litter and copper. The long-term broiler litter research plots were established in 1997 and the copper fertility research plots were not established until 2009. Both experiments use a randomized complete lock design with the broiler litter experiment composed of four replications and eight treatments at the following rates: 0, 1, 2, 3, 4, 5, 6, and 7 tons BL * ac^-1. The copper experiment was designed with four replications and three treatments at the following rates: 0, 10, …

Earthworm Differences Among Mound Positions In Native Prairies In The Ozark Highlands, Carlee D. Hensley, Kristofor R. Brye, Mary C. Savin, Lisa S. Wood, Edward E. Gbur

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Mounded, tallgrass prairie ecosystems are a unique, endangered ecosystem due to severe disturbance for agricultural production and/or urban expansion. Limited research exists on earthworm (Lumbricus terrestris) dynamics in mounded, tallgrass prairies, particularly in the Ozark Highlands region of northwestern Arkansas. The objectives of this study were to evaluate the effects of soil moisture regime (SMR), landscape position (LP), and sample date on earthworm densities in the top 20 cm of soil in two mounded, native prairies in the Ozark Highlands region of northwestern Arkansas. Juvenile and total earthworm densities were five times greater (P < .05) in the udic- and aquic-inter-mound combinations than any other SMR-LP combination in October 2018 and May 2019, respectively. Adult earthworm densities were two times greater (P < .05) in the aquic-inter-mound at Searles Prairie than any other SMR-LP combination in either mounded prairie. Juvenile earthworm densities were three times greater (P < .05) in May 2019 than in May 2018 at Chesney Prairie, but adult and total earthworm density did not change over the same period of time. Combined across all data, juvenile and total earthworm densities were positively correlated (P …