Life Sciences Commons^™

A Cross-Ecoregion Evaluation Of Nitrogen Fixation And Denitrification In Streams And Rivers Of The United States Of America, Amy Marcarelli, Erin K. Eberhard, Michelle Kelly, Kevin Nevorski

Michigan Tech Publications

It is typically assumed that dinitrogen (N2) fixation and denitrification are mutually exclusive processes in riverine ecosystems because N2 fixation is favored in high light, low nitrogen (N) environments but denitrification is favored under anoxic, high N conditions. Yet recent work in marine and lake ecosystems has demonstrated that N2 fixation can happen under high N conditions and in sediments, challenging this assumption. We conducted a cross-ecoregion study to test the hypothesis that N2 fixation and denitrification would co-occur in streams and rivers across a range of reactive N concentrations. Between 2017 and 2019, we sampled 30 streams in 13 …

Β-Hydroxy-Β-Methylbutyrate Supplementation Promotes Antitumor Immunity In An Obesity Responsive Mouse Model Of Pancreatic Ductal Adenocarcinoma, Michael F. Coleman, Kristyn A. Liu, Alexander J. Pfeil, Suhas K. Etigunta, Xiaohu Tang, Salvador Fabela, Et. Al.

Michigan Tech Publications

Pancreatic cancer (PDAC) is a deadly disease, exacerbated by obesity, which lacks effective therapeutic interventions. Most PDAC has a limited response to immune- and chemotherapy. Treating PDAC is made additionally challenging by the rapid emergence of muscle wasting and cachexia, which predict poor response to several therapies. We have found that dietary supplementation with β-hydroxy-β-methylbutyrate promotes immunosurveillance in PDAC tumors and protects muscle. This dietary supplement has the potential to be an important adjuvant in PDAC therapy, opening the doors to immunotherapy response.

Volume 4, Chapter 1-17: Aquatic And Wet Marchantiophyta, Order Blasiales, Janice M. Glime

Bryophyte Ecology Subchapters

No abstract provided.

Earthworm And Soil Data For Ottawa National Forest, Blair Orr

Michigan Tech Research Data

Two types of analysis were run.

I. Probability of finding L. terrestris in habitat types of the Ottawa National Forests.

Abstract: Parts of the Ottawa National Forest (ONF) provide suitable habitat for invasive earthworms. Extensive earthworm invasion is a relatively recent event on the ONF and this study captures the current state of the earthworm invasion through a four-stage invasive species distribution model (iSDM). The random distribution of earthworms indicates early colonization by earthworms which is moderated by habitat (forest type, soil group, and drainage class). CART modeling was used to determine probability of earthworm invasion. The CART model had …

Genome-Wide Snp Identification In Fraxinus Linking Genetic Characteristics To Tolerance Of Agrilus Planipennis, Cecelia E. Hale, Mark A. Jordan, Gloria Iriarte, Kirk Broders, Andrew J. Storer, Vamsi J. Nalam, Jordan M. Marshall

Michigan Tech Publications

Ash (Fraxinus spp.) is one of the most widely distributed tree genera in North America. Populations of ash in the United States and Canada have been decimated by the introduced pest Agrilus planipennis (Coleoptera: Buprestidae; emerald ash borer), having negative impacts on both forest ecosystems and economic interests. The majority of trees succumb to attack by A. planipennis, but some trees have been found to be tolerant to infestation despite years of exposure. Restriction site-associated DNA (RAD) sequencing was used to sequence ash individuals, both tolerant and susceptible to A. planipennis attack, in order to identify single nucleotide polymorphism (SNP) …

Adsorption Of Perfluorooctanoic Acid (Pfoa) And Perfluorooctanesulfonic Acid (Pfos) By Aluminum-Based Drinking Water Treatment Residuals, Zhiming Zhang, Dibyendu Sarkar, Rupali Datta, Yang Deng

Michigan Tech Publications

Per- and polyfluoroalkyl substances (PFAS) represent a family of emerging persistent organic pollutants. Cost-effective remediation of PFAS contamination via chemical or biochemical degradation is challenging due to their extremely high stability. This study reports the removal of two representative PFAS species, perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS), from water by adsorption using aluminum-based water treatment residuals (Al-WTR), a non-hazardous waste generated during the process of drinking water treatment by alum salts. Rapid adsorption of PFOA and PFOS onto Al-WTR followed a pseudo 2nd order kinetic pattern. Lower pH facilitated the adsorption process with a faster adsorption rate and greater …

Water Chemistry And Aquatic Vegetation Data From Les Cheneaux Islands, Northern Lake Huron, Michigan, Usa, 2016-2018, Colin Brooks, Amy Marcarelli, Casey Huckins, Amanda Grimm

Michigan Tech Research Data

Remote sensing approaches that could identify species of submerged aquatic vegetation (SAV) and measure their extent in lake littoral zones would greatly enhance their study and management, especially if they can provide faster or more accurate results than traditional field methods. Remote sensing with multispectral sensors can provide this capability, but SAV identification with this technology must address the challenges of light extinction in aquatic environments where chlorophyll, dissolved organic carbon, and suspended minerals can affect water clarity and the strength of the sensed light signal. Here, we present environmental data collected to support a study using an unmanned aerial …

The Future Of Wildlife Conservation Funding: What Options Do U.S. College Students Support?, Lincoln R. Larson, Markus Nils Peterson, Richard Von Furstenberg, Victoria R. Vayer, Kangjae Jerry Lee, Daniel Y. Choi, Matt Kelly, Richelle Winkler, Et. Al

Michigan Tech Publications

Insufficient funding is a major impediment to conservation efforts around the world. In the United States, a decline in hunting participation threatens sustainability of the “user-pay, public benefit” model that has supported wildlife conservation for nearly 100 years, forcing wildlife management agencies to contemplate alternative funding strategies. We investigated support for potential funding options among diverse college students, a rapidly expanding and politically active voting bloc with a potentially powerful influence on the future of conservation. From 2018 to 2020, we surveyed 17,203 undergraduate students at public universities across 22 states. Students preferred innovative approaches to conservation funding, with 72% …

Vulnerability Of Street Trees In Upper Midwest Cities To Climate Change, Leslie A. Brandt, Gary R. Johnson, Eric A. North, Jack Faje, Annamarie Rutledge

Michigan Tech Publications

Urban trees play an important role in helping cities adapt to climate change, but also are vulnerable to changes in climate themselves. We developed an approach for assessing vulnerability of urban tree species and cultivars commonly planted in cities in the United States Upper Midwest to current and projected climate change through the end of the 21st century. One hundred seventy-eight tree species were evaluated for their adaptive capacity to a suite of current and future-projected climate and urban stressors using a weighted scoring system based on an extensive literature review. These scores were then evaluated and adjusted by leading …

Tragic Trade-Offs Accompany Carnivore Coexistence In The Modern World, Jeremy T. Bruskotter, John A. Vucetich, Sophie L. Gilbert, Neil H. Carter, Kelly A. George

Michigan Tech Publications

Two vital policy aims—biodiversity conservation and food production—are increasingly in conflict. Efforts to evaluate trade-offs between agriculture and conservation have shaped scholarly discourse around two broad strategies to agricultural production that seek to either “share” land with biodiversity or “spare” land from agriculture. However, efforts to negotiate these trade-offs are challenged by rising concern for the welfare of individual animals, both wild and domestic. We use recent efforts to “coexist” with large carnivores to illustrate how sharing and sparing strategies both create tragic, and often unacknowledged trade-offs between livestock production and carnivore conservation. We conclude the best means of conserving …

Can Deliberative Democracy Favor A Flourishing Relationship Between Humans And Carnivores?, John A. Vucetich, Jeremy T. Bruskotter, David W. Macdonald

Michigan Tech Publications

There is considerable interest in improving participatory governance in decision-making processes for the conservation of biodiversity and management of conflicts between humans and wildlife. Among the various modes of participatory governance, deliberative democracy has received virtually no attention for decisions focused on conserving biodiversity. This is surprising given that deliberative democracy is an important branch of democratic theory and is associated with decision-making processes that have been successfully applied to a wide range of complicated decisions across diverse cultural settings. Moreover, deliberative democracy has several distinctive properties that would seem to make it well-suited for many conservation decisions. First, deliberative …

Flea The Flyentist: What Is An Invasive Species?, Molly Fitzgerald

Open Access Books

No abstract provided.

Corrigendum: Bel1-Like Homeodomain Protein Blh6a Is A Negative Regulator Of Cald5h2 In Sinapyl Alcohol Monolignol Biosynthesis In Poplar (Front. Plant Sci., (2021), 12, (695223), 10.3389/Fpls.2021.695223), Qiao Wang, Xinren Dai, Hongying Pang, Yanxia Cheng, Xiong Huang, Hui Li, Xiaojing Yan, Fachuang Lu, Hairong Wei, Ronald R. Sederoff, Quanzi Li

Michigan Tech Publications

When originally published, the article title contained a typographical error. The correct gene name should be “CAld5H2” instead of “CAl5H2” as originally published. The correct title is “BEL1-like Homeodomain Protein BLH6a is a Negative Regulator of CAld5H2 in Sinapyl Alcohol Monolignol Biosynthesis in Poplar.” The authors state that this does not change the scientific conclusions of the article in any way. The original article has been updated.

Body Size, Trophic Position, And The Coupling Of Different Energy Pathways Across A Saltmarsh Landscape, Friedrich W. Keppeler, Jill A. Olin, Paola C. López-Duarte, Michael J. Polito, Linda M. Hooper-Bùi, Sabrina S. Taylor, Nancy N. Rabalais, F. Joel Fodrie, Brian J. Roberts, R. Eugene Turner, Charles W. Martin, Olaf P. Jensen

Michigan Tech Publications

Body size is considered an important structuring mechanism of food webs because consumers are usually larger and more mobile than their prey and may couple energy among habitats. We explored the links among trophic position (TP), body size, and the coupling of different energy channels (phytoplankton and C4-marsh plants) in a saltmarsh landscape in the northern Gulf of Mexico—a dynamic system considered weakly shaped by biotic interactions. Body size was positively associated with TP, and this relationship was stronger in the phytoplankton pathway vs. the C4-marsh pathway. There was a gradual increase in the coupling of phytoplankton and C4-marsh plants …

The Third Generation Of Pan-Canadian Wetland Map At 10 M Resolution Using Multisource Earth Observation Data On Cloud Computing Platform, Masoud Mahdianpari, Brian Brisco, Jean Granger, Fariba Mohammadimanesh, Bahram Salehi, Saeid Homayouni, Laura Bourgeau-Chavez

Michigan Tech Publications

Development of the Canadian Wetland Inventory Map (CWIM) has thus far proceeded over two generations, reporting the extent and location of bog, fen, swamp, marsh, and water wetlands across the country with increasing accuracy. Each generation of this training inventory has improved the previous results by including additional reference wetland data and focusing on processing at the scale of ecozone, which represent ecologically distinct regions of Canada. The first and second generations attained relatively highly accurate results with an average approaching 86% though some overestimated wetland extents, particularly of the swamp class. The current research represents a third refinement of …

Incorporation Of Biochar To Improve Mechanical, Thermal And Electrical Properties Of Polymer Composites, Chinmoyee Das, Sandeep Tamrakar, Alper Kiziltas, Xinfeng Xie

Michigan Tech Publications

The strive for utilization of green fillers in polymer composite has increased focus on application of natural biomass-based fillers. Biochar has garnered a lot of attention as a filler material and has the potential to replace conventionally used inorganic mineral fillers. Biochar is a carbon rich product obtained from thermochemical conversion of biomass in nitrogen environment. In this review, current studies dealing with incorporation of biochar in polymer matrices as a reinforcement and conductive filler were addressed. Each study mentioned here is nuanced, while addressing the same goal of utilization of biochar as a filler. In this review paper, an …

Improved Forest Management As A Natural Climate Solution: A Review, Lilli Kaarakka, Meredith Cornett, Grant Domke, Todd A. Ontl, Laura E. Dee

Michigan Tech Publications

Natural climate solutions (NCS), a set of land management, conservation and restoration practices aimed at mitigating climate change, have been introduced as cost-effective strategies to increase carbon (C) sequestration in terrestrial ecosystems. Improved forest management (IFM) has been identified as one NCS for working forests with substantial climate change mitigation potential. However, there is a disconnect between the policy and carbon markets context and the scientific evidence for verifiable C benefits. Further, forest soil C—the largest forest C pool—has largely been excluded from current forest management guidelines and has not been included in the IFM discourse. Herein, we assess the …

Coarse Woody Debris Decomposition Assessment Tool: Model Validation And Application, Zhaohua Dai, Carl C. Trettin, Andrew Burton, Martin Jurgensen, Deborah S. Page-Dumroese, Brian T. Forschler, Jonathan S. Schilling, Daniel L. Lindner

Michigan Tech Publications

Coarse woody debris (CWD) is a significant component of the forest biomass pool; hence a model is warranted to predict CWD decomposition and its role in forest carbon (C) and nutrient cycling under varying management and climatic conditions. A process-based model, CWDDAT (Coarse Woody Debris Decomposition Assessment Tool) was calibrated and validated using data from the FACE (Free Air Carbon Dioxide Enrichment) Wood Decomposition Experiment utilizing pine (Pinus taeda), aspen (Populous tremuloides) and birch (Betula papyrifera) on nine Experimental Forests (EF) covering a range of climate, hydrology, and soil conditions across the continental USA. The model predictions were evaluated against …

The Motion Of Trees In The Wind: A Data Synthesis, Toby D. Jackson, Sarab Sethi, Ebba Dellwik, Nikolas Angelou, Amanda Bunce, Tim Van Emmerik, Mark Rudnicki, Et. Al.

Michigan Tech Publications

Interactions between wind and trees control energy exchanges between the atmosphere and forest canopies. This energy exchange can lead to the widespread damage of trees, and wind is a key disturbance agent in many of the world's forests. However, most research on this topic has focused on conifer plantations, where risk management is economically important, rather than broadleaf forests, which dominate the forest carbon cycle. This study brings together tree motion time-series data to systematically evaluate the factors influencing tree responses to wind loading, including data from both broadleaf and coniferous trees in forests and open environments. We found that …

Activation Of Orexin System Stimulates Camkii Expression, Yuanyuan Fan, Enshe Jiang, Huanjia Gao, Jeremy Bigalke, Bojun Chen, Chunxiu Yu, Qinghui Chen, Zhiying Shan

Michigan Tech Publications

Hyperactivity of the orexin system within the paraventricular nucleus (PVN) has been shown to contribute to increased sympathetic nerve activity (SNA) and blood pressure (BP) in rodent animals. However, the underlying molecular mechanisms remain unclear. Here, we test the hypothesis that orexin system activation stimulates calcium/calmodulin-dependent kinase II (CaMKII) expression and activation, and stimulation of CaMKII expressing PVN neurons increases SNA and BP. Real-time PCR and/or western blot were carried out to test the effect of orexin-A administration on CaMKII expression in the PVN of normal Sprague Dawley (SD) rats and orexin receptor 1 (OX1R) expressing PC12 cells. Immunostaining was …

A Systems Biology Approach Identifies A Regulator, Bplerf1, Of Cold Tolerance In Betula Platyphylla, Kaiwen Lv, Wenqi Wu, Hairong Wei, Guifeng Liu

Michigan Tech Publications

Cold is an abiotic stress that can greatly affect the growth and survival of plants. Here, we reported that an AP2/ERF family gene, BplERF1, isolated from Betula platyphylla played a contributing role in cold stress tolerance. Overexpression of BplERF1 in B. platyphylla transgenic lines enhanced cold stress tolerance by increasing the scavenging capability and reducing H2O2 and malondialdehyde (MDA) content in transgenic plants. Construction of BplERF-mediated multilayered hierarchical gene regulatory network (ML-hGRN), using Top-down GGM algorithm and the transcriptomic data of BplERF1 overexpression lines, led to the identification of five candidate target genes of BplERF1 which include MPK20, …

Bel1-Like Homeodomain Protein Blh6a Is A Negative Regulator Of Cal5h2 In Sinapyl Alcohol Monolignol Biosynthesis In Poplar, Qiao Wang, Xinren Dai, Hongying Pang, Yanxia Cheng, Xiong Huang, Hui Li, Hairong Wei, Et. Al.

Michigan Tech Publications

Lignin is one of the major components of xylem cell walls in tree stems. The lignin in the wood of most flowering plants (dicotyledonous angiosperms) is typically polymerized from three monolignol precursors, coniferyl alcohol, sinapyl alcohol, and p-coumaroyl alcohol, resulting in guaiacyl (G), syringyl (S), and hydroxyphenyl (H) subunits, respectively. In this study, we focus on the transcriptional regulation of a coniferaldehyde 5-hydroxylase (CAld5H2) gene, which encodes a key enzyme for sinapyl alcohol biosynthesis. We carried out a yeast one-hybrid (Y1H) screen to identify candidate upstream transcription factors (TFs) regulating CAld5H2. We obtained 12 upstream TFs …

Successful Management Of Invasive Rats Across A Fragmented Landscape, Sarah K. Barney, Devin R. Leopold, Kainana Francisco, David J. Flaspohler, Tadashi Fukami, Christian P. Giardina, Daniel S. Gruner, Jessie L. Knowlton, William C. Pitt, Erin E. Wilson Rankin

Michigan Tech Publications

Summary Introduced mammalian predators are responsible for the decline and extinction of many native species, with rats (genus Rattus) being among the most widespread and damaging invaders worldwide. In a naturally fragmented landscape, we demonstrate the multi-year effectiveness of snap traps in the removal of Rattus rattus and Rattus exulans from lava-surrounded forest fragments ranging in size from 10 ha. Relative to other studies, we observed low levels of fragment recolonization. Larger rats were the first to be trapped, with the average size of trapped rats decreasing over time. Rat removal led to distinct shifts in the foraging height and …

Nutrient Limitation Of Algae And Macrophytes In Streams: Integrating Laboratory Bioassays, Field Experiments, And Field Data, Christopher A. Mebane, Andrew M. Ray, Amy Marcarelli

Michigan Tech Publications

Successful eutrophication control strategies need to address the limiting nutrient. We conducted a battery of laboratory and in situ nutrient-limitation tests with waters collected from 9 streams in an agricultural region of the upper Snake River basin, Idaho, USA. Laboratory tests used the green alga Raphidocelis subcapitata, the macrophyte Lemna minor (duckweed) with native epiphytes, and in situ nutrient-limitation tests of periphyton were conducted with nutrient-diffusing substrates (NDS). In the duckweed/epiphyte test, P saturation occurred when concentrations reached about 100 μg/L. Chlorophyll a in epiphytic periphyton was stimulated at low P additions and by about 100 μg/L P, epiphytic periphyton …

Chromosome-Level Genome Assembly Of A Regenerable Maize Inbred Line A188, Guifang Lin, Cheng He, Jun Zheng, Dal Hoe Koo, Ha Le, Huakun Zheng, Hairong Wei, Et. Al.

Michigan Tech Publications

Background: The maize inbred line A188 is an attractive model for elucidation of gene function and improvement due to its high embryogenic capacity and many contrasting traits to the first maize reference genome, B73, and other elite lines. The lack of a genome assembly of A188 limits its use as a model for functional studies. Results: Here, we present a chromosome-level genome assembly of A188 using long reads and optical maps. Comparison of A188 with B73 using both whole-genome alignments and read depths from sequencing reads identify approximately 1.1 Gb of syntenic sequences as well as extensive structural variation, including …

Scientific Access Into Mercer Subglacial Lake: Scientific Objectives, Drilling Operations And Initial Observations, John C. Priscu, Jonas Kalin, John Winans, Timothy Campbell, Matthew R. Siegfried, Mark Skidmore, Trista J. Vick‐Majors, Et. Al.

Michigan Tech Publications

The Subglacial Antarctic Lakes Scientific Access (SALSA) Project accessed Mercer Subglacial Lake using environmentally clean hot-water drilling to examine interactions among ice, water, sediment, rock, microbes and carbon reservoirs within the lake water column and underlying sediments. A ∼0.4 m diameter borehole was melted through 1087 m of ice and maintained over ∼10 days, allowing observation of ice properties and collection of water and sediment with various tools. Over this period, SALSA collected: 60 L of lake water and 10 L of deep borehole water; microbes >0.2 μm in diameter from in situ filtration of ∼100 L of lake water; …

Coarse Woody Debris Decomposition Assessment Tool: Model Development And Sensitivity Analysis, Zhaohua Dai, Carl C. Trettin, Andrew Burton, Martin Jurgensen, Deborah S. Page-Dumroese, Brian T. Forschler, Jonathan S. Schilling, Daniel L. Lindner

Michigan Tech Publications

Coarse woody debris (CWD) is an important component in forests, hosting a variety of organisms that have critical roles in nutrient cycling and carbon (C) storage. We developed a process-based model using literature, field observations, and expert knowledge to assess woody debris decomposition in forests and the movement of wood C into the soil and atmosphere. The sensitivity analysis was conducted against the primary ecological drivers (wood properties and ambient conditions) used as model inputs. The analysis used eighty-nine climate datasets from North America, from tropical (14.2° N) to boreal (65.0° N) zones, with large ranges in annual mean temperature …

Transcriptome-Wide Identification And Characterization Of Micrornas In Diverse Phases Of Wood Formation In Populus Trichocarpa, Ruiqi Wang, Mengxuan Reng, Shuanghui Tian, Cong Liu, He Cheng, Yingying Liu, Hairong Wei, Et. Al.

Michigan Tech Publications

We applied miRNA expression profiling method to Populus trichocarpa stems of the three developmental stages, primary stem (PS), transitional stem (TS), and secondary stem (SS), to investigate miRNA species and their regulation on lignocellulosic synthesis and related processes. We obtained 892, 872, and 882 known miRNAs and 1727, 1723, and 1597 novel miRNAs, from PS, TS, and SS, respectively. Comparisons of these miRNA species among different developmental stages led to the identification of 114, 306, and 152 differentially expressed miRNAs (DE-miRNAs), which had 921, 2639, and 2042 candidate target genes (CTGs) in the three respective stages of the same order. …

Volume 4, Chapter 1-18: Aquatic And Wet Marchantiophyta, Order Lunulariales, Janice M. Glime

Bryophyte Ecology Subchapters

No abstract provided.

The Diverse Roles Of Cytokinins In Regulating Leaf Development, Wenqi Wu, Kang Du, Xiangyang Kang, Hairong Wei

Michigan Tech Publications

Leaves provide energy for plants, and consequently for animals, through photosynthesis. Despite their important functions, plant leaf developmental processes and their underlying mechanisms have not been well characterized. Here, we provide a holistic description of leaf developmental processes that is centered on cytokinins and their signaling functions. Cytokinins maintain the growth potential (pluripotency) of shoot apical meristems, which provide stem cells for the generation of leaf primordia during the initial stage of leaf formation; cytokinins and auxins, as well as their interaction, determine the phyllotaxis pattern. The activities of cytokinins in various regions of the leaf, especially at the margins, …