Life Sciences Commons^™

Alloscorpiops Wongpromi Sp. N. From Thailand And Laos (Scorpiones: Euscorpiidae: Scorpiopinae), František Kovařík, Michael E. Soleglad, Ondřej Košulič

Euscorpius

Alloscorpiops wongpromi sp. n. from Thailand and Laos is described and compared with all other species of the genus Alloscorpiops Vachon, 1980. A key is presented to all species of the genus. A. wongpromi sp. n. is characterized mainly by a higher number of trichobothria on the patella, which has 33–37 external and 21–22 ventral trichobothria, and a dark-colored telson. The subgenus Laoscorpiops Lourenço, 2013 is synonymized with the nominotypical subgenus, because it was based on misinterpretation of the trichobothrial pattern, with two ventral accessory trichobothria on the chela regarded as part of the external Eb series.

Response Of Male Centruroides Vittatus (Scorpiones: Buthidae) To Aerial And Substrate-Borne Chemical Signals, Steffany B. Steinmetz, Karen C. Bost, Douglas D. Gaffin

Euscorpius

Chemical signaling promotes mate location throughout numerous animal taxa. In this study we investigated the possibility that striped scorpions, Centruroides vittatus, use chemical signaling in the form of odor plumes or substrate-borne deposits as communication channels. A Y-shaped arena was constructed to test scorpions’ use of air-borne chemical cues in the detection of potential mates. A second, circular behavioral choice chamber was used to test male scorpions’ responses to female deposits by direct substrate contact. Male scorpions showed no tendency to move toward the female in tests of air-borne chemical transmission but they did demonstrate behavior associated with chemical …

Scorpiofauna Of Kashan (Esfahan Province, Iran) (Arachnida: Scorpiones), Valerio Vignoli, František Kovařík, Pierangelo Crucitti

Euscorpius

This work contains the results of the zoological expedition of the Società Romana di Scienze Naturali to Kashan, in Iran. Four days of intensive field research provided interesting data on the community structure of scorpions of Kashan. Thirty-two scorpion specimens, all belonging to the family Buthidae, are listed and ecological notes are included. Iranobuthus krali Kovařík, 1997, Kraepelinia palpator (Birula, 1903) and Polisius persicus Fet, Capes & Sissom, 2001 were found for the first time in the examined localities. The species collected belong to different ecomorpho-types, characteristic for the particular geographic position of Kashan. We report presence of two species …

A Powerful New Light Source For Ultraviolet Detection Of Scorpions In The Field, Graeme Lowe, Steven R. Kutcher, Doug Edwards

Euscorpius

We describe a new portable solid-state ultraviolet (UV) light that should greatly improve the ease and effectiveness of fluorescent detection of scorpions in the field. The design is based on an array of InGaN light emitting diodes (LEDs). It is in-expensive, lightweight, simple to implement, energetically efficient and mechanically rugged. Scorpion cuticular fluorescence excited by an LED array is 13-fold brighter than excitation by previously available fluorescent tube lamps drawing the same current from the battery.

Smokejumper Obituary: Roberts, Ralph B. (Missoula 1955), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

A Comparison Of Infaunal Community Structure Between Pre- And Post Construction Sampling Of Artificial Fdot Rock- Pile Reefs In Broward County, Florida, Joan Lorraine Guerra

HCNSO Student Theses and Dissertations

Artificial reefs (ARs) are generally created with the intention of increasing local fish populations, biodiversity and corresponding habitat productivity, most often in support of commercial and recreational uses (e.g., diving and fishing). Numerous studies have investigated the communities that develop on artificial reefs. By contrast, far less research has focused on the surrounding infaunal communities, which represent critical trophic resources for many species that populate both natural and artificial reefs, and which may be affected both by AR deployment and the organisms that subsequently recruit to them. This study compared infaunal organism abundances at four sites between the inner and …

Super-Killers: Environmental Isolates That Antagonize Pathogenic Vibrio, Abigail Joy

Honors Projects

Vibrio are members of a bacterial group that thrive in diverse aquatic environments including on the surface of aquatic animals, free-living in the water column, and in association with suspended particles. The total Vibrio counts in the coastal ocean ranges from 10³-10⁵ per milliliter of water depending on seasons and water temperature. Although many different species of Vibrio persist in the water column, pathogenic strains, such as Vibrio cholerae and Vibrio parahaemolyticus are absent or rare in marine environments. We hypothesize that the low abundance of these pathogenic species may be due to interspecific competition among environmental …

Selection Of Reference Genes For Rt-Qpcr Analysis In A Predatory Biological Control Agent, Coleomegilla Maculata (Coleoptera: Coccinellidae), Chunxiao Yang, Huipeng Pan, Jeffrey Edward Noland, Deyong Zhang, Zhanhong Zhang, Yong Liu, Xuguo Zhou

Entomology Faculty Publications

Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a reliable technique for quantifying gene expression across various biological processes, of which requires a set of suited reference genes to normalize the expression data. Coleomegilla maculata (Coleoptera: Coccinellidae), is one of the most extensively used biological control agents in the field to manage arthropod pest species. In this study, expression profiles of 16 housekeeping genes selected from C. maculata were cloned and investigated. The performance of these candidates as endogenous controls under specific experimental conditions was evaluated by dedicated algorithms, including geNorm, Normfinder, BestKeeper, and ΔCt method. In addition, …

Genomic Legacy Of The African Cheetah, Acinonyx Jubatus, Pavel Dobrynin, Shiping Liu, Gaik Tamazian, Zijun Xiong, Andrey A. Yurchenko, Ksenia Krasheninnikova, Sergey Kliver, A. Schmidt-Kunzel, Klaus-Peter Koepfli, Warren E. Johnson, Lukas F. K. Kuderna, Raquel Garcia-Perez, Marc De Manuel, Ricardo Godinez, Aleksey Komissarov, Alexey Makunin, Vladimir Brukhin, Weilin Qiu, Long Zhou, Fang Li, Jian Yi, Carlos A. Driscoll, Agostinho Antunes, T. K. Oleksyk, Eduardo Eizirik, Polina Perelman, Melody E. Roelke, David E. Wildt, Mark Diekhans, Tomas Marques-Bonet, Laurie Marker, Jong Bhak, Jun Wang, Guojie Zhang, Stephen J. O'Brien

Biology Faculty Articles

Background

Patterns of genetic and genomic variance are informative in inferring population history for human, model species and endangered populations.

Results

Here the genome sequence of wild-born African cheetahs reveals extreme genomic depletion in SNV incidence, SNV density, SNVs of coding genes, MHC class I and II genes, and mitochondrial DNA SNVs. Cheetah genomes are on average 95 % homozygous compared to the genomes of the outbred domestic cat (24.08 % homozygous), Virunga Mountain Gorilla (78.12 %), inbred Abyssinian cat (62.63 %), Tasmanian devil, domestic dog and other mammalian species. Demographic estimators impute two ancestral population bottlenecks: one >100,000 years …

Possible Synergistic Effects Of Thymol And Nicotine Against Crithidia Bombi Parasitism In Bumble Bees, Olivia Masi Biller, Lynn S. Adler, Rebecca E. Irwin, Caitlin Mcallister, Evan C. Palmer-Young

Dartmouth Scholarship

Floral nectar contains secondary compounds with antimicrobial properties that can affect not only plant-pollinator interactions, but also interactions between pollinators and their parasites. Although recent work has shown that consumption of plant secondary compounds can reduce pollinator parasite loads, little is known about the effects of dosage or compound combinations. We used the generalist pollinator Bombus impatiens and its obligate gut parasite Crithidia bombi to study the effects of nectar chemistry on host-parasite interactions. In two experiments we tested (1) whether the secondary compounds thymol and nicotine act synergistically to reduce parasitism, and (2) whether dietary thymol concentration affects parasite …

Chemo-Enzymatic Synthesis Of Site-Specific Isotopically Labeled Nucleotides For Use In Nmr Resonance Assignment, Dynamics And Structural Characterizations, Andrew P. Longhini, Regan M. Leblanc, Owen Becette, Carolina Salguero, Christoph H. Wunderlich, Bruce A. Johnson, Victoria M. D'Souza, Christoph Kreutz, T. Kwaku Dayle

Advanced Science Research Center

Stable isotope labeling is central to NMR studies of nucleic acids. Development of methods that incorporate labels at specific atomic positions within each nucleotide promises to expand the size range of RNAs that can be studied by NMR. Using recombinantly expressed enzymes and chemically synthesized ribose and nucleobase, we have developed an inexpensive, rapid chemo-enzymatic method to label ATP and GTP site specifically and in high yields of up to 90%.We incorporated these nucleotides into RNAs with sizes ranging from 27 to 59 nucleotides using in vitro transcription: A-Site (27 nt), the iron responsive elements (29 nt), a fluoride riboswitch …

Exploration Of The Range Overlap Between Cynomys Leucurus And Cynomys Ludovicianus, Kenneth D. Choi

Honors Theses

The ranges of two species of prairie dogs, Cynomys leucurus and Cynomys ludovicianus, overlap in a narrow area in the western Great Plains in Wyoming. We used GIS software to map their distributions and infer land cover preferences within and on either side of the zone of overlap. In general, both species used habitats in proportion to their availability and did not display strong selection of their expected vegetation types (based on the literature). However, both species of prairie dogs were overrepresented in areas of human settlements and crops, which may indicate that these species prefer human-modified habitats, or perhaps …

L3 Auditory Interneuron Responses To Dual-Frequency Calling Songs In Female Crickets (Acheta Domesticus), Jeong Bin Lee

Honors Theses

Female crickets exhibit positive phonotaxis in response to calling songs with specific carrier frequencies (4-5 kHz), syllable periods (SP; 50-70ms), and other parameters. Among a number of ascending prothoracic auditory neurons identified, only L3 has been proposed to be involved in SP selective phonotaxis. L3 responds with selective decrement to 5 kHz stimulus with attractive SPs. Furthermore, L3 is highly tuned to 16 kHz auditory stimulus, which has been correlated with aversive phonotactic behaviors. L3’s cellular responses to simultaneous 16 kHz and 5 kHz stimuli were examined through extracellular recording, thereby further establishing the connection between L3’s selective decrement and …

Using Hexoskin Wearable Technology To Obtain Body Metrics In A Trail Hiking Setting, Jeffrey Montes, Tori M. Stone, Jacob W. Manning, Damon Mccune, Debra K. Tacad, Jack C. Young, Mark Debeliso Phd, James W. Navalta

International Journal of Exercise Science

International Journal of Exercise Science 8(4): 425-430, 2015. Use of wearable technology to obtain various body metrics appears to be a trending phenomenon. However there is very little literature supporting the notion that these apparatuses can be used for research purposes in the field. The purpose of this study was to utilize Hexoskin wearable technology shirts (HxS) to obtain data in a pilot study using a trail hiking situation. Ten individuals (male, n = 4, female n = 6) volunteered to participate. On the first day, volunteers completed two approximately flat trail hikes at a self-preferred pace with a 15-minute …

Mesobuthus Eupeus (C.L. Koch, 1839) (Scorpiones: Buthidae) In Turkey, Ayşegül Karataş, Ahmet Karataş

Euscorpius

Distribution of Mesobuthus eupeus (C.L. Koch, 1839) (Scorpiones: Buthidae) in Turkey is investigated and new distributional records are given. In addition, data on the variation of the number of pectinal teeth, and some ecological features of M. eupeus are provided.

Proceedings Of The 3rd Scorpiology Symposium (American Arachnological Society, 28th Annual Meeting), Victor Fet, Michael E. Soleglad

Euscorpius

Table of Contents

Victor Fet and Douglas D. Gaffin. Preface p. i

Karen C. Bost and Douglas D. Gaffin. Sand scorpion home burrow navigation in the laboratory p. 1

Douglas D. Gaffin and Mark E. Walvoord. Scorpion peg sensilla: are they the same or are they different? p. 7

J. Zachary Porterfield, Douglas D. Gaffin, Caitlin Porterfield and Curtis Johnston. Screening for scorpions: A non-invasive approach to tracking the movements of arachnids in sand p. 17

Tsunemi Yamashita. Surface activity, biomass, and phenology of the striped scorpion, Centruroides vittatus (Scorpiones: Buthidae) in Arkansas, USA p. 25

C. Neal McReynolds. Temporal …

Prey Capture Behavior In The East African Scorpions Parabuthus Leiosoma (Ehrenberg, 1828) And P. Pallidus Pocock, 1895 (Scorpiones: Buthidae), Jan O. Rein

Euscorpius

Prey capture behavior in Parabuthus leiosoma (Ehrenberg, 1828) and P. pallidus Pocock, 1895 was studied in the laboratory. The behavioral components involved in prey capture were identified and an ethogram is presented. The occurrence of the different prey capture components are analyzed and discussed.

Oxidative Stress In Avian Embyros, Toshi Tsunekage

Dissertations

Oxidative stress has been implicated in mediating trade-offs in the evolution of life histories. Oxidative stress results from an imbalance in the production of free radicals and an organism’s antioxidant defenses. Higher metabolic rates associated with more rapid growth and shorter development periods may increase oxidative stress and accumulated cellular damage in embryonic tissues. In my dissertation I explored oxidative stress and antioxidant defenses in avian embryos. I measured levels of oxidative stress in tissues of different stage embryos of Japanese quail (Coturnix japonica), and tested if patterns of lipid peroxidation could be explained by changes in the developing embryo’s …

Development And Evaluation Of A Multi-Year Fractional Surface Water Data Set Derived From Active/Passive Microwave Remote Sensing Data, Ronny Schroeder, Kyle C. Mcdonald, Bruce D. Chapman, Katherine Jensen, Erika Podest, Zachary D. Tessler, Theodore J. Bohn, Reiner Zimmermann

Publications and Research

The sensitivity of Earth’s wetlands to observed shifts in global precipitation and temperature patterns and their ability to produce large quantities of methane gas are key global change questions. We present a microwave satellite-based approach for mapping fractional surface water (FW) globally at 25-km resolution. The approach employs a land cover-supported, atmospherically-corrected dynamic mixture model applied to 20+ years (1992–2013) of combined, daily, passive/active microwave remote sensing data. The resulting product, known as Surface WAter Microwave Product Series (SWAMPS), shows strong microwave sensitivity to sub-grid scale open water and inundated wetlands comprising open plant canopies. SWAMPS’ FW compares favorably (R2 …

Genetic Modification Of Human Mesenchymal Stem Cells Helps To Reduce Adiposity And Improve Glucose Tolerance In An Obese Diabetic Mouse Model., Sabyasachi Sen, Cleyton C Domingues, Carol Rouphael, Cyril Chou, Chul Kim, Nagendra Yadava

Medicine Faculty Publications

INTRODUCTION: Human mesenchymal stem cells (MSCs) are multipotent cells that can differentiate into fat, muscle, bone and cartilage cells. Exposure of subcutaneous abdominal adipose tissue derived AD-MSCs to high glucose (HG) leads to superoxide accumulation and up-regulation of inflammatory molecules. Our aim was to inquire how HG exposure affects MSCs differentiation and whether the mechanism is reversible.

METHODS: We exposed human adipose tissue derived MSCs to HG (25 mM) and compared it to normal glucose (NG, 5.5 mM) exposed cells at 7, 10 and 14 days. We examined mitochondrial superoxide accumulation (Mitosox-Red), cellular oxygen consumption rate (OCR, Seahorse) and gene …

Smokejumper Obituary: Fleming, John L. (Missoula 1949), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Connectivity Of Coastal And Oceanic Ecosystems: Pelagic Habitat Use By Juvenile Reef Fishes In The Gulf Of Mexico, Katie Bowen

HCNSO Student Theses and Dissertations

The assemblage structure, abundance, biomass, and vertical distribution of juvenile reef fishes in the offshore pelagic habitat of the northern Gulf of Mexico are described as part of the NOAA-supported Offshore Nekton Sampling and Analysis Program. The results presented here are from a 3-month, continuous sampling series in 2011 in which discrete depth strata from 0 to 1500 m were sampled using a 10-m² MOCNESS midwater trawl. This is the first study to examine pelagic juvenile reef fish distributions across the entire oceanic northern Gulf of Mexico seaward of the continental shelf break after the Deepwater Horizon Oil Spill. …

Linker Histone H1 And H3k56 Acetylation Are Antagonistic Regulators Of Nucleosome Dynamics, Morgan Bernier, Yi Luo, Kingsley C. Nwokelo, Michelle Goodwin, Sarah J. Dreher, Pei Zhang, Mark R. Parthun, Yvonne N. Fondufe-Mittendorf, Jennifer J. Ottesen, Michael G. Poirier

Molecular and Cellular Biochemistry Faculty Publications

H1 linker histones are highly abundant proteins that compact nucleosomes and chromatin to regulate DNA accessibility and transcription. However, the mechanisms that target H1 regulation to specific regions of eukaryotic genomes are unknown. Here we report fluorescence measurements of human H1 regulation of nucleosome dynamics and transcription factor (TF) binding within nucleosomes. H1 does not block TF binding, instead it suppresses nucleosome unwrapping to reduce DNA accessibility within H1-bound nucleosomes. We then investigated H1 regulation by H3K56 and H3K122 acetylation, two transcriptional activating histone post translational modifications (PTMs). Only H3K56 acetylation, which increases nucleosome unwrapping, abolishes H1.0 reduction of TF …

Mobile Broadband In Nebraska, Charlotte Narjes, Rebecca J. Vogt, Cullen Robbins

Cornhusker Economics

Mobile Broadband Coverage in Nebraska

• Is mobile broadband coverage available throughout Nebraska?
• Is there a difference between rural and urban areas with respect to mobile wireless speed?
• Should mobile wireless coverage be classified as broadband?

These are some of the questions that were answered by an August 2015 Nebraska Public Service Commission (PSC) report. The study, which began in October 2013, was part of the Nebraska Broadband Initiative efforts to facilitate the integration of broadband and information technology into state and local economics.

A Comparison Of Gastrointestinal Bacterial Population Between Indoor Cats And Outdoor Cats, Farhana Binti Ikmal Hisham

Honors Theses

This study used fecal samples from four indoor cats and four indoor-outdoor cats in order to identify the microbial communities in the gut of cats. This information was then used to compare the microbial phyla between both groups for any differences. Total microbial DNA was isolated from each fecal sample, and the 16S rRNA gene was sequenced using Illumina MiSeq high throughput method. The sequences were identified using the bioinformatics program mothur. The results show that indoor cats had a more diverse microbial community as compared to outdoor cats. Indoor cat samples had 26% more microbial species, and eight more …

A Review Of The Genus Isometrus Ehrenberg, 1828 (Scorpiones: Buthidae) With Descriptions Of Four New Species From Asia And Australia, František Kovařík

Euscorpius

Four new species of the genus Isometrus are described: Isometrus (Reddyanus) bilyi from Australia, I. (R.) petrzelkai from Vietnam and Thailand, I. (R.) problematicus from India and I. (R.) khammamensis from India. Keys are presented to all species of both subgenera of Isometrus. Lectotypes are designated for I. (I.) thurstoni Pocock, 1893 and I. (R.) assamensis Oates, 1888. I. (I.) sankariensis Tikader & Bastawade, 1983 is synonymized with I. (I.) thurstoni Pocock, 1893. First time records include I. (I.) thurstoni Pocock, …

High-Level Systematics And Phylogeny Of The Extant Scorpions (Scorpiones: Orthosterni), Michael E. Soleglad, Victor Fet

Euscorpius

A number of authors (e. g. Birula, 1917a, 1917b; Mello-Leitão, 1945; Stockwell, 1989) addressed above-level systematics of extant scorpions, and accepted the grouping of scorpion families in several superfamilies. At the same time, Kjellesvig-Waering (1986) classified all extant scorpions under the same superfamily, Scorpionoidea. Sissom (1990) and Fet et al. (2000) did not list any superfamilies, considering the systematic situation above family (and often at the family level as well) unresolved. Most recently, Lourenço (2000a) listed six superfamilies, largely following the unpublished but important study of Stockwell (1989). The goal of this paper is to address scorpion systematics and phylogeny …

The Scorpion Sternum: Structure And Phylogeny (Scorpiones: Orthosterni), Michael E. Soleglad, Victor Fet

Euscorpius

The structure of the sternum of all major Recent scorpion groups is analyzed in detail. Based on this analysis, two fundamental sternum types are identified, described and illustrated, type 1 and type 2. These sternum types are distinguished by criteria based on external and internal structural features. The sternum types described herein are offered as a replacement for the various characterizations used throughout the last 140 years which emphasize only gross overall shape and proportions. Phylogenetic and taxonomic ramifications of these new sternal types are discussed. The Carboniferous fossil scorpion Palaeopisthacanthus schucherti Petrunkevitch is assigned to sternum type 1 …

Oregon Wine Board Meeting Minutes December 8, 2015, Oregon Wine Board

Oregon Wine Board Documents

These meeting minutes list individuals in attendance at the December 8, 2015 Oregon Wine Board (OWB) meeting, held at the Hotel Vintage in Portland, Oregon. The Board went into Executive Session prior to calling the meeting to order. The finance, education, and marketing committees provided reports. The Board discussed funding grant proposals from the 11^th International Terroir Congress and the Oregon Wine History Archive. The Board also discussed nominations for industry awards and elected a new slate of Directors to chair OWB's Executive Committee in 2016. The meeting lasted 4 hours 13 minutes (including the time spent in Executive …

Design And Testing Of Novel Anthrax Vaccines Utilizing A Tobacco Mosaic Virus Expression System, Ryan C. Mccomb

KGI Theses and Dissertations

Anthrax is a potentially fatal disease caused by the bacteria Bacillus anthracis. Infection and disease occur after spores gain entry into the body, germinate into vegetative bacteria, and produce toxin. Bacillus anthracis spores have been engineered as bioweapons and have been used repeatedly in warfare and terrorism to inflict casualties in military and civilian populations. Currently, only one vaccine has been approved for prevention of anthrax in the United States. This vaccine is an undefined product that is difficult to produce, requires a long vaccination schedule, and is reactogenic. Efforts to make an improved anthrax vaccine are being pursued. …