Life Sciences Commons^™

Comparative Analysis Of Prostate Cancer Gene Regulatory Networks Via Hub Type Variation, Pegah Khosravi, Vahid H. Gazestani, Mohammad Akbarzadeh, Samira Mirkhalaf, Mehdi Sadeghi, Bahram Goliaei

Publications and Research

Background

Prostate cancer is one of the most widespread cancers in men and is fundamentally a genetic disease. Identifying regulators in cancer using novel systems biology approaches will potentially lead to new insight into this disease. It was sought to address this by inferring gene regulatory networks (GRNs). Moreover, dynamical analysis of GRNs can explain how regulators change among different conditions, such as cancer subtypes.

Methods

In our approach, independent gene regulatory networks from each prostate state were reconstructed using one of the current state-of-art reverse engineering approaches. Next, crucial genes involved in this cancer were highlighted by analyzing each …

Histopathology And Oxidative Stress Analysis Of Concomitant Misoprostol And Celecoxib Administration, Derek E. Murrell, James W. Denham, Sam Harirforoosh

Pharmacy Faculty Articles and Research

Nonsteroidal anti-inflammatory drugs (NSAIDs), non-selective or selective inhibitors of cyclooxygenase (COX-1 and -2), reduce pain and inflammation associated with arthritic diseases. Celecoxib, a COX-2-selective inhibitor providing decreased gastric injury relative to on-selective NSAIDs, is commonly prescribed. Misoprostol, a prostaglandin analog, supplements NSAID-inhibited prostaglandin levels. As concomitant celecoxib and misoprostol administration has been shown to intensify renal adverse effects, this article examined the influence of concomitant administration on hepatic histopathology, oxidative stress, and celecoxib concentration. On days 1 and 2, rat groups (n = 6) were gavaged twice daily (two groups with vehicle and two groups with 100 μg/kg misoprostol). From …

Detection And Tracking Of T Cells In Time-Lapse Imaging, Cody Arbuckle, Milton L. Greenberg, Erik J. Linstead

Mathematics, Physics, and Computer Science Faculty Articles and Research

The effective classification and tracking of cells obtained from modern staining techniques has significant limitations due to the necessity of having to train and utilize a human expert in the field who must manually identify each cell in each slide. Often times these slides are filled with noise cells that are not of particular interest to the researcher. The use of computational methods has the ability to effectively and efficiently enhance image quality, as well as identify and track target cell types over large data sets. Here we present a computational approach to the in vitro tracking of T cells …

A Conservative Isoleucine To Leucine Mutation Causes Major Rearrangements And Cold Sensitivity In Klentaq1 Dna Polymerase, Eugene Y. Wu, Amanda R. Walsh, Emma C. Materne, Emily P. Hiltner, Bryan Zielinski, Bill R. Miller Iii, Lily Mawby, Carol A. Parish, Wayne M. Barnes, Milko B. Kermekchiev

Biology Faculty Publications

Assembly of polymerase chain reactions at room temperature can sometimes lead to low yields or unintentional products due to mispriming. Mutation of isoleucine 707 to leucine in DNA polymerase I from Thermus aquaticus substantially decreases its activity at room temperature without compromising its ability to amplify DNA. To understand why a conservative change to the enzyme over 20 Å from the active site can have a large impact on its activity at low temperature, we solved the X-ray crystal structure of the large (5′-to-3′ exonuclease-deleted) fragment of Taq DNA polymerase containing the cold-sensitive mutation in the ternary (E–DNA–ddNTP) and binary …

Ph Dependent Antibiotic Resistance Of An Alkaliphilic, Halotolerant Bacterium Isolated From Soap Lake, Washington, Tiffany Charlynn Edwards

Masters Theses

"Soap Lake, located in Washington State, is a meromictic, soda lake. Many bacterial isolates retrieved from Soap Lake have been noted to possess resistance to multiple antibiotics. A likely explanation for the wide range of antibacterial resistance exhibited by these strains is due to the impact of high alkalinity on the antibiotics themselves and not due to the presence of antibiotic resistance genes. The aim of our study was to determine if select antibiotics are effective against Halomonas eurialkalitoleranis, a bacterium capable of growth over a wide range of neutral to alkaline pH values, to investigate the influence of alkalinity …

Detecting Diesel Particulate Matter Using Real Time Monitoring Under The Influence Of An Exhaust Fan System, Yasir Helal Alghamdi

Masters Theses

"Diesel Particulate Matter (DPM) is a complex mixture of diesel exhaust gas that consists of carbon, ash, metallic abrasion particles, sulfates and silicates. The diesel soot particle includes a solid core made of elemental carbon, and organic carbon compound attached to the surface of the soot particle. The main source of DPM is diesel exhaust gas. The National Institute for Occupational Safety and Health (NIOSH) and the Environmental Protection Agency (EPA) have determined that DPM is the source of most of the emissions of carbon monoxide, carbon dioxide, oxide of nitrogen, and hydrocarbons in underground coal, metal and non metal …

Healing Of Bone Defects In A Rodent Calvarial Defect Model Using Strong Porous Bioactive Glass (13-93) Scaffolds, Yinan Lin

Masters Theses

"The main objective of this project was to evaluate the capacity of strong porous silicate (13-93) bioactive glass scaffolds prepared by a robocasting technique to regenerate bone and stimulate angiogenesis in a rat calvarial defect model. The scaffolds were created with the same grid-like microstructure but in a variety of formulations: (i) as-fabricated, (ii) pretreated in an aqueous phosphate solution to convert the glass surface to hydroxyapatite, (iii) loaded with bone morphogenetic protein-2 (BMP2) (1 µg per scaffold), and (iv) doped with copper (0.4-2.0 wt. % CuO). When compared to the as-fabricated scaffolds, the pretreated scaffolds enhanced bone regeneration at …

Extreme Tolerance And Developmental Buffering Of Uv-C Induced Dna Damage In Embryos Of The Annual Killifish Austrofundulus Limnaeus, Josiah Tad Wagner, Jason E. Podrabsky

Biology Faculty Publications and Presentations

Free-living aquatic embryos are often at risk of exposure to ultraviolet radiation (UV-R). Successful completion of embryonic development depends on efficient removal of DNA lesions, and thus many aquatic embryos have mechanisms to reverse DNA lesions induced by UV-R. However, little is known of how embryos that are able to enter embryonic dormancy may respond to UV-R exposure and subsequent DNA damage. Embryos of the annual killifish Austrofundulus limnaeus are unique among vertebrates because their normal embryonic development includes (1) a complete dispersion of embryonic blastomeres prior to formation of the definitive embryonic axis, and (2) entry into a state …

Effects Of Sex And Mycorrhizal Fungi On Gas Exchange In The Dioecious Salt Marsh Grass Distichlis Spicata, Kassandra Reuss-Schmidt, Todd N. Rosenstiel, Sally R. Rogers, Allie G. Simpson, Sarah M. Eppley

Biology Faculty Publications and Presentations

Premise of research. In dioecious plant species, males and females often differ in physiology, and mycorrhizal fungal relationships are likely to influence these differences. However, few data are available on the potential role of mycorrhizal fungi in altering sex-specific physiology and population sex ratios of dioecious plant species.

Methodology. In this study, we measured leaf gas exchange in a multifactorial greenhouse experiment with and without mycorrhizal fungal additions and under field conditions in Distichlis spicata, a dioecious C4 salt marsh grass, displaying extreme spatial sex ratio variation.

Pivotal results. We found a significant interaction between gas exchange, plant sex, and …