Life Sciences Commons^™

Treatment Thresholds For Bollworm (Helicoverpa Zea) In Dual-Gene Bt Cotton, Kristen Carter

All Theses

Dual-gene Bt cotton has reduced the need for insecticide treatments for bollworm, Helicoverpa zea (Boddie), compared with original single-gene Bt technology. Bollgard II¨ (Monsanto, St. Louis, MO) and WideStrike¨ (Dow AgroSciences, Indianapolis, IN), both produce the Cry1Ac protein and a second protein, Cry2Ab or Cry1F, respectively. These dual-gene Bt cottons provide enhanced control of lepidopteran pests, but remain less than 100% effective against bollworm, particularly when population pressure is high. Current recommended treatment thresholds for bollworm on cotton in South Carolina are as follows: treat with insecticides when three or more large larvae are found per 100 plants or when …

Behavioral Changes For African Americans To Improve Health, Embrace Culture, And Minimize Disparities, Dashiel J. Geyen Ed.D., M.P.H.

The Bridge: Interdisciplinary Perspectives on Legal & Social Policy

In spite of significant advances in the prevention, diagnoses, and treatment of most chronic diseases, there is evidence that racial and ethnic people of color tend to receive lower quality of care and less preventive interventions than the majority members of a community. While our health system should improve its efforts at addressing and reducing disproportionality in health care, African Americans can make behavioral changes through diet and exercise that will reduce their susceptibility to chronic illnesses. This article first reviews the prevalence, disparities, and characteristics of chronic diseases impacting the African American population. Second, it discusses culturally connected practices …

Intraspecific Diversity In Sinningia Speciosa (Gesneriaceae: Sinningieae), And Possible Origins Of The Cultivated Florist's Gloxinia, David Zaitlin

Kentucky Tobacco Research and Development Center Faculty Publications

BACKGROUND AND AIMS: The florist's gloxinia is a familiar houseplant in the Gesneriaceae, the botanical family that includes the African violet (Saintpaulia) and other ornamental species. The gloxinia's wild progenitor is Sinningia speciosa (Lodd.) Hiern, a Brazilian endemic. Although it has been cultivated for almost 200 years, little is known about the genetic diversity in S. speciosa, how the wild populations relate to one another or even where the cultivated forms originated. Using available wild collections, preliminary phenetic and phylogenetic investigations were conducted to elucidate the interspecific relationships within S. speciosa and to infer the origins of the cultivars.

METHODOLOGY: …

Biochemical Reconstitution Of Hemorrhagic-Fever Arenavirus Envelope Glycoprotein-Mediated Membrane Fusion, Celestine J. Thomas, Sundaresh Shankar, Hedi E. Casquilho-Gray, Joanne York, Stephen Sprang, Jack H. Nunberg

Biological Sciences Faculty Publications

The membrane-anchored proteins of enveloped viruses form labile spikes on the virion surface, primed to undergo large-scale conformational changes culminating in virus-cell membrane fusion and viral entry. The prefusion form of these envelope glycoproteins thus represents an important molecular target for antiviral intervention. A critical roadblock to this endeavor has been our inability to produce the prefusion envelope glycoprotein trimer for biochemical and structural analysis. Through our studies of the GPC envelope glycoprotein of the hemorrhagic fever arenaviruses, we have shown that GPC is unique among class I viral fusion proteins in that the mature complex retains a stable signal …

Effects Of Length Limits On Sexually Size Dimorphic Fishes, Peter J. Spirk

School of Natural Resources: Dissertations, Theses, and Student Research

Length limits are used by fishery managers as a method to alter size structure of fish populations. Unfortunately, biological differences between fish sexes (i.e., sexual-size dimorphism) may lead to sex-specific rates of recruitment, growth, and mortality. The addition of angler harvest to most aquatic systems likely accentuates differences in sex-specific rates by selectively harvesting the fastest-growing and largest fish from a population. The first objective of this study was to document the extent of sexual-size dimorphism for white bass and walleye at a Nebraska reservoir. Growth rates were similar between male and female white bass although male white bass were …

Proteome Analyses Of Strains Cyanothece Atcc 51142 And Pcc 7822 Of The Diazotrophic Cyanobacterium Cyanothece Sp. Under Culture Conditions Resulting In Enhanced H2 Production., Uma K. Aryal, Stephen J. Callister, Sujata Mishra, Xiaohui Zhang, Janani I. Shutthanandan, Thomas E. Angel, Anil K. Shukla, Matthew E. Monroe, Ronald J. Moore, David W. Koppenhaal, Richard D. Smith, Louis A. Sherman

Department of Biological Sciences Faculty Publications

Cultures of the cyanobacterial genus Cyanothece have been shown to produce high levels of biohydrogen. These strains are diazotrophic and undergo pronounced diurnal cycles when grown under N₂-fixing conditions in light-dark cycles. We seek to better understand the way in which proteins respond to these diurnal changes, and we performed quantitative proteome analysis of Cyanothecesp. strains ATCC 51142 and PCC 7822 grown under 8 different nutritional conditions. Nitrogenase expression was limited to N₂-fixing conditions, and in the absence of glycerol, nitrogenase gene expression was linked to the dark period. However, glycerol induced expression of nitrogenase during …

Septin Phosphorylation And Coiled-Coil Domains Function In Cell And Septin Ring Morphology In The Filamentous Fungus Ashbya Gossypii, Rebecca A. Meseroll, Patricia Occhipinti, Amy S. Gladfelter

Dartmouth Scholarship

Septins are a class of GTP-binding proteins conserved throughout many eukaryotes. Individual septin subunits associate with one another and assemble into heteromeric complexes that form filaments and higher-order structures in vivo. The mechanisms underlying the assembly and maintenance of higher-order structures in cells remain poorly understood. Septins in several organisms have been shown to be phosphorylated, although precisely how septin phosphorylation may be contributing to the formation of high-order septin structures is unknown. Four of the five septins expressed in the filamentous fungus, Ashbya gossypii, are phosphorylated, and we demonstrate here the diverse roles of these phosphorylation sites …

Comparison Of Three Collection Techniques For Capture Of Coleoptera, With An Emphasis On Saproxylic Species, In Great Smoky Mountains National Park, Usa, Michael L. Ferro, Matthew L. Gimmel, Kyle E. Harms, Christopher E. Carlton

Insecta Mundi

Collection methods and/or habitats sampled influence how many and which species are captured during entomological surveys. Here we compare Coleoptera catches among three survey activities, each using a single collection method, at the same study sites in Great Smoky Mountains National Park, USA. Activities included: short-term flight intercept trapping (FITs); sifting/Berlese funneling of leaf litter and extremely decayed downed coarse woody debris; and using emergence chambers containing coarse woody debris of various decay classes. In total, 2472 adult beetle specimens, representing 217 lowest identifiable taxa within 164 genera and 42 families, were collected during the FIT survey. Each survey activity …

Characterization Of Head Transcriptome And Analysis Of Gene Expression Involved In Caste Differentiation And Aggression In Odontotermes Formosanus (Shiraki), Qiuying Huang, Pengdong Sun, Xuguo Zhou, Chaoliang Lei

Entomology Faculty Publications

Background

The subterranean termite Odontotermes formosanus (Shiraki) is a serious insect pest of trees and dams in China. To date, very little is known about genomic or transcriptomic data for caste differentiation and aggression in O. formosanus. Hence, studies on transcriptome and gene expression profiling are helpful to better understand molecular basis underlying caste differentiation and aggressive behavior in O. formosanus.

Methodology and Principal Findings

Using the Illumina sequencing, we obtained more than 57 million sequencing reads derived from the heads of O. formosanus. These reads were assembled into 116,885 unique sequences (mean size = 536 bp). …

Projected Climate Change Impacts On The Hydrology And Temperature Of Pacific Northwest Rivers, Huan Wu, John S. Kimball, Marketa M. Elsner, Nate Mantua, Robert F. Adler, Jack Arthur Stanford

Biological Sciences Faculty Publications

A dominant river-tracing-based streamflow and temperature (DRTT) model was developed by coupling stream thermal dynamics with a source-sink routing model. The DRTT model was applied using 1/16 degree (similar to 6 km) resolution gridded daily surface meteorology inputs over a similar to 988,000 km(2) Pacific Northwest (PNW) domain to produce regional daily streamflow and temperature simulations from 1996 to 2005. The DRTT results showed favorable performance for simulation of daily stream temperature (mean R-2 = 0.72 and root-mean-square error = 2.35 degrees C) and discharge (mean R-2 = 0.52 and annual relative error 14%) against observations from 12 PNW streams. …

Guam, Guam, Institute Of Child Nutrition. Child Nutrition Archives.

Oral History Project (all interviews)

This oral history interview was conducted with Ignacio Santos, Dina Lorenzo, Jesse Rosario, and DeAnndra Chargualaf from the Guam Department of Education, who were visiting NFSMI for training, on November 29, 2012. In this interview they discuss the challenges and benefits of child nutrition programs on Guam.

Localizing Brain Regions Associated With Female Mate Preference Behavior In A Swordtail, Ryan Y. Wong, Mary E. Ramsey, Molly E. Cummings

Biology Faculty Publications

Female mate choice behavior is a critical component of sexual selection, yet identifying the neural basis of this behavior is largely unresolved. Previous studies have implicated sensory processing and hypothalamic brain regions during female mate choice and there is a conserved network of brain regions (Social Behavior Network, SBN) that underlies sexual behaviors. However, we are only beginning to understand the role this network has in pre-copulatory female mate choice. Using in situ hybridization, we identify brain regions associated with mate preference in female Xiphophorus nigrensis, a swordtail species with a female choice mating system. We measure gene expression in …

The Principal Genetic Determinants For Nasopharyngeal Carcinoma In China Involve The Hla Class I Antigen Recognition Groove, Minzhong Tang, J. A. Lautenberger, Xiaojiang Gao, Efe Sezgin, Sher L. Hendrickson, Jennifer L. Troyer, Victor A. David, Li Guan, Carl Mcintosh, Xiuchan Guo, Yuming Zheng, Jian Liao, Hong Deng, Michael Malasky, Bailey Kessing, Cheryl Winkler, Mary Carrington, Guy De The, Yi Zeng, Stephen J. O'Brien

Biology Faculty Articles

Nasopharyngeal carcinoma (NPC) is an epithelial malignancy facilitated by Epstein-Barr Virus infection. Here we resolve the major genetic influences for NPC incidence using a genome-wide association study (GWAS), independent cohort replication, and high-resolution molecular HLA class I gene typing including 4,055 study participants from the Guangxi Zhuang Autonomous Region and Guangdong province of southern China. We detect and replicate strong association signals involving SNPs, HLA alleles, and amino acid (aa) variants across the major histocompatibility complex-HLA-A, HLA –B, and HLA -C class I genes (P_{HLA-A-aa-site-62}= 7.4×10⁻²⁹; P _{HLA-B-aa-site-116}= 6.5×10⁻¹⁹; P _{HLA-C-aa-site-156} = 6.8×10 …

Divergent Carbon Dynamics Under Climate Change In Forests With Diverse Soils, Tree Species, And Land Use Histories, Robert M. Scheller, Alec M. Kretchun, Steve Van Tuyl, Kenneth L. Clark, Melissa S. Lucash, John Hom

Environmental Science and Management Faculty Publications and Presentations

Accounting for both climate change and natural disturbances—which typically result in greenhouse gas emissions—is necessary to begin managing forest carbon sequestration. Gaining a complete understanding of forest carbon dynamics is, however, challenging in systems characterized by historic over-utilization, diverse soils and tree species, and frequent disturbance. In order to elucidate the cascading effects of potential climate change on such systems, we projected forest carbon dynamics, including soil carbon changes, and shifts in tree species composition as a consequence of wildfires and climate change in the New Jersey pine barrens (NJPB) over the next 100 years. To do so, we used …

Similarities Between Line Fishing And Baited Stereo-Video Estimations Of Length-Frequency: Novel Application Of Kernel Density Estimates, Timothy J. Langlois, Benjamin R. Fitzpatrick, David V. Fairclough, Corey B. Wakefield, Sybrand Alexander Hesp, Dianne L. Mclean, Euan S. Harvey, Jessica J. Meeuwig

Fisheries Research Articles

Age structure data is essential for single species stock assessments but length-frequency data can provide complementary information. In south-western Australia, the majority of these data for exploited species are derived from line caught fish. However, baited remote underwater stereo-video systems (stereo-BRUVS) surveys have also been found to provide accurate length measurements. Given that line fishing tends to be biased towards larger fish, we predicted that, stereo-BRUVS would yield length-frequency data with a smaller mean length and skewed towards smaller fish than that collected by fisheries-independent line fishing. To assess the biases and selectivity of stereo-BRUVS and line fishing we compared …

New Technologies For Whole Wheat Processing: Addressing Milling And Storage Issues, Andres F. Doblado-Maldonado

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Whole wheat flour production and demand has increased dramatically during the last decade due to evidence supporting the benefits of whole grains in the diet. Hence, the food industry has provided a wide variety of new whole grain products. There are unique challenges that accompany whole wheat flour production, especially related to milling and storage. The present thesis provides new strategies on the adaptation of new technologies to overcome whole wheat processing issues. These issues are first discussed in a literature review and then followed by three research studies. In the first study, retail whole wheat flours were evaluated for …

Site Selection By Migratory Shorebirds In Oregon Estuaries Over Broad And Fine Spatial Scales, Aileen Kilpatrick Miller

Dissertations and Theses

Many migratory shorebirds rely on estuaries as stop-over sites to refuel during migration, and the loss of stop-over sites is a primary threat to shorebird populations on the West Coast of the United States (e.g. Calidris alpina pacifica, C. mauri). Conservation and research has focused on the largest of these sites; however, smaller estuaries also host thousands of migratory shorebirds. Furthermore, the reasons for site selection are largely unknown. Estuarine inter-tidal microhabitats are non-uniform and both abiotic and biotic factors may serve as predictors of whether an abundance of shorebirds will use a site. I investigated shorebird site selection on …

Omer, Charles Henry, 1865-1937 (Mss 436), Manuscripts & Folklife Archives

Manuscript Collection Finding Aids

Finding aid only for Manuscripts Collection 436. Personal journals of Charles Henry Omer of Union County, Kentucky. In this daily record Omer records information about his farming, family and church activities, the weather, as well as prices for food, timber and farm supplies.

Global Trophic Position Comparison Of Two Dominant Mesopelagic Fish Families (Myctophidae, Stomiidae) Using Amino Acid Nitrogen Isotopic Analyses, C. Anela Choy, Peter C. Davison, Jeffrey C. Drazen, Adrian Flynn, Elizabeth J. Gier, Joel C. Hoffman, Jennifer P. Mcclain-Counts, Todd W. Miller, Brian N. Popp, Steve W. Ross, Tracey Sutton

Marine & Environmental Sciences Faculty Articles

The δ15N values of organisms are commonly used across diverse ecosystems to estimate trophic position and infer trophic connectivity. We undertook a novel cross-basin comparison of trophic position in two ecologically well-characterized and different groups of dominant mid-water fish consumers using amino acid nitrogen isotope compositions. We found that trophic positions estimated from the δ15N values of individual amino acids are nearly uniform within both families of these fishes across five global regions despite great variability in bulk tissue δ15N values. Regional differences in the δ15N values of phenylalanine confirmed that bulk tissue δ15N values reflect region-specific water mass biogeochemistry …

Morphological And Genetic Changes Induced By Excess Zn In Roots Of Medicago Truncatula A17 And A Zn Accumulating Mutant, Ricky W. Lewis, Guiliang Tang, David H. Mcnear

Plant and Soil Sciences Faculty Publications

BACKGROUND: Nutrient fluxes associated with legume-rhizobia symbioses are poorly understood and little is known regarding the influence of abiotic stresses on development and maintenance of N-fixing nodules and root system architecture (RSA). We examined effects of Zn on nodule development and structure, root architecture, and expression of nodulation-related miRNAs in Medicago truncatula and the mutant, raz (requires additional Zn).

FINDINGS: Excess Zn increased root and shoot associated Zn in both genotypes, however, raz plants had lower root associated Zn than WT plants. Roots of raz plants exposed to excess Zn had less volume, surface area, and total length compared to …

The Relationship Between Sleep Deprivation, Food Motivation, And Energy Intake In Normal-Weight And Obese Females, Lora Light Romney

Theses and Dissertations

Objective: Sleep deprivation has been proposed as a potential correlate of obesity, particularly influencing energy intake. Therefore, the purpose of this study was to compare neural indices of attention related to food motivation and energy intake in normal-weight and obese women under two separate sleep conditions: 1) sleep-restricted (<5 hours) and 2) recommended sleep (~8 hours). This study used a combined cross-over and ex post facto design with condition order counter-balanced. Methods: Twenty-two normal-weight (age=30.9±9.5y, BMI=22.0±1.6 kg/m2) and 18 obese (age=29.7±10.7 y, BMI=36.4±5.3 kg/m2) women completed both sleep conditions. To confirm sleep levels, participants recorded sleep quality and quantity via sleep logs and wore a wrist actigraph. Following each condition, participants reported to the laboratory under the same fed state (energy shake ~10% of total daily needs) to verify they followed the sleep protocol. Subsequently, motivation for food was tested using electroencephalogram (EEG); participants completed a computerized passive-viewing task of food and flowers, while event-related brain potentials (ERPs) were recorded. After EEG testing, participants continued their normal routine but recorded all energy intake using weighed food scales. There were no instructions or limitations on dietary intake. Analyses included P300 and LPP amplitudes in response to picture type, total next day energy intake, and energy intake by several periods of the day. Results: Participants averaged 4.7±0.4 hours of sleep during the sleep-restricted condition and 7.7±0.3 hours during the recommended sleep condition (F=1057.02; P<0.0001). There was no group*condition interaction for next day food motivation (P300: F<2.896, P>0.09; LPP: F<2.967, P>0.093). Next day total energy intake also did not differ by group*condition (F=1.81; P=0.187). When participants were pooled, there was no difference in energy intake by sleep condition (F=0.00; P=0.953). However, when participants’ energy intake was analyzed during the lunch period (following testing to 1:30pm) there was a …

Improved Understanding Of Factors Influencing The Re-Emergence Of Goss's Bacterial Wilt And Blight Of Corn, Craig B. Langemeier

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Goss’s bacterial wilt and blight (Goss’s wilt) is a serious and sometimes severe disease of corn. Goss’s wilt was first identified in Dawson County Nebraska in 1969. Today Goss’s wilt can be found in two countries including the U.S. and Canada, and twelve states including Nebraska, Iowa, North Dakota, South Dakota, Minnesota, Illinois, Indiana, Texas, Kansas, Colorado, Wisconsin and Wyoming. Goss’s wilt was observed in Nebraska throughout the 1970’s, and from the early 1980’s until recently developed only sporadically. Around 2006, a re-emergence of the disease was observed in western Nebraska, northeast Colorado, and southeast Wyoming. Since then, reports of …

Predicting Coral Recruitment In Palau’S Complex Reef Archipelago, Yimnang Golbuu, Eric C. Wolanski, Jacques Wasai Idechong, Steven Victor, Adelle Lukes Isechal, Noelle Wenty Oldiais, David Idip, Robert H. Richmond, Robert Van Woesik

Ocean Engineering and Marine Sciences Faculty Publications

Reproduction and recruitment are key processes that replenish marine populations. Here we use the Palau archipelago, in the western Pacific Ocean, as a case study to examine scales of connectivity and to determine whether an oceanographic model, incorporating the complex reef architecture, is a useful predictor of coral recruitment. We tested the hypothesis that the reefs with the highest retention also had the highest densities of juvenile coral density from 80 field sites. Field comparisons showed a significant correlation between the densities of juvenile Acropora colonies and total larval recruitment derived from the model (i.e., calculated as the sum of …

Pharmacokinetics And Brain Uptake In The Rhesus Monkey Of A Fusion Protein Of Arylsulfatase A And A Monoclonal Antibody Against The Human Insulin Receptor, Ruben J. Boado, Jeff Zhiqiang Lu, Eric Ka-Wai Hui, Rachita K. Sumbria, William M. Pardridge

Pharmacy Faculty Articles and Research

Metachromatic leukodystrophy (MLD) is a lysosomal storage disorder of the brain caused by mutations in the gene encoding the lysosomal sulfatase, arylsulfatase A (ASA). It is not possible to treat the brain in MLD with recombinant ASA, because the enzyme does not cross the blood-brain barrier (BBB). In the present investigation, a BBB-penetrating IgG-ASA fusion protein is engineered and expressed, where the ASA monomer is fused to the carboxyl terminus of each heavy chain of an engineered monoclonal antibody (MAb) against the human insulin receptor (HIR). The HIRMAb crosses the BBB via receptor-mediated transport on the endogenous BBB insulin receptor, …

U.S. Drought Monitor, November 27, 2012, Eric Luebehusen

United States Agricultural Commodities in Drought Archive

Drought map of U.S. for November 27, 2012 (11/27/12) plus: U.S. crop areas experiencing drought (map), Approximate percentage of crop located in drought, by state (bar graph), Percent of crop area located in drought, past 52 weeks (line graph) for: Hay, Cattle, Winter wheat.

Stopover Decisions Of Migratory Shorebirds: An Assessment Of Habitat Use, Food Availability, Behavior And Phenology, Ryan Stutzman

School of Natural Resources: Dissertations, Theses, and Student Research

Habitat loss and alteration from land use change, species invasion, and more recently, climate change has reduced biodiversity and ecosystem function worldwide. Habitat decisions have important implications to individual fitness as well as population dynamics and community structure. Resource limitation, predation, competition, and unfavorable abiotic conditions all have the potential to influence survival and future reproductive potential. Understanding how changes to ecosystem structure and function impact species and populations of conservation concern is essential for conservation delivery to be effective. Similar to many migratory species, shorebird populations are declining worldwide and declines may be related to the loss of important …

The Evolution Of Host Specificity In The Vertebrate Gut Symbiont Lactobacillus Reuteri, Steven Frese

Department of Food Science and Technology: Dissertations, Theses, and Student Research

The vertebrate gut is home to one of the densest populations of life on Earth. This microbial community has a profound effect on host health, nutrition, development, behavior, and evolution. However, very little is known about how these microbes have evolved with their vertebrate hosts, how and whether they select hosts or how they remain associated with their hosts. Recent work identified Lactobacillus reuteri as an organism that is composed of host-specific sub-populations, each population associated with a different host animal. Representatives from each host-associated population were tested for their ability to colonize gnotobiotic mice, which only rodent strains could …

Late Embryogenesis Abundant Proteins Protect Human Hepatoma Cells During Acute Desiccation, Shumin Li, Nilay Chakraborty, Apurva Borcar, Michael A. Menze, Mehmet Toner, Steven C. Hand

Faculty Research & Creative Activity

Expression of late embryogenesis abundant (LEA) proteins is highly correlated with desiccation tolerance in anhydrobiotic animals, selected land plants, and bacteria. Genes encoding two LEA proteins, one localized to the cytoplasm/nucleus (AfrLEA2) and one targeted to mitochondria (AfrLEA3m), were stably transfected into human HepG2 cells. A trehalose transporterwas used for intracellular loading of this disaccharide. Cellswere rapidly and uniformly desiccated to low water content (<0.12 g H2O/g dry weight) with a recently developed spin-drying technique. Immediately on rehydration, control cells without LEA proteins or trehalose exhibited 0% membrane integrity, comparedwith 98% in cells loaded with trehalose and expressing AfrLEA2 or AfrLEA3m; surprisingly, AfrLEA3m without trehalose conferred 94% protection. Cell proliferation across 7 d showed an 18-fold increase for cells dried with AfrLEA3m and trehalose, compared with 27-fold for nondried controls. LEA proteins dramatically enhance desiccation tolerance in mammalian cells and offer the opportunity for engineering biostability in the dried state.

Late Embryogenesis Abundant Proteins Protect Human Hepatoma Cells During Acute Desiccation., Shumin Li, Nilay Chakraborty, Apurva Borcar, Michael Menze, Mehmet Toner, Steven Hand

Faculty and Staff Scholarship

Expression of late embryogenesis abundant (LEA) proteins is highly correlated with desiccation tolerance in anhydrobiotic animals, selected land plants, and bacteria. Genes encoding two LEA proteins, one localized to the cytoplasm/nucleus (AfrLEA2) and one targeted to mitochondria (AfrLEA3m), were stably transfected into human HepG2 cells. A trehalose transporterwas used for intracellular loading of this disaccharide. Cellswere rapidly and uniformly desiccated to low water content (< 0.12 g H2O/g dry weight) with a recently developed spin-drying technique. Immediately on rehydration, control cells without LEA proteins or trehalose exhibited 0% membrane integrity, comparedwith 98% in cells loaded with trehalose and expressing AfrLEA2 or AfrLEA3m; surprisingly, AfrLEA3m without trehalose conferred 94% protection. Cell proliferation across 7 d showed an 18-fold increase for cells dried with AfrLEA3m and trehalose, compared with 27-fold for nondried controls. LEA proteins dramatically enhance desiccation tolerance in mammalian cells and offer the opportunity for engineering biostability in the dried state.

Sinusoidal Endothelial Dysfunction In Non-Alcoholic Fatty Liver Disease., Sandhya Lakshmi Gopalakrishnan

Department of Biochemistry: Dissertations, Theses, and Student Research

Non-alcoholic fatty liver disease (NAFLD) is an asymptomatic increasingly common disorder that affects liver metabolism and is often the precursor for liver pathologies such as fibrosis, cirrhosis and hepato-cellular carcinoma. The liver sinusoidal endothelial cells act as a liver sieve by allowing macromolecules and chylomicrons to traverse through their fenestrations (sieve plates) to hepatocytes. Since liver sinusoidal endothelial cells (LSEC) regulate serum derived macromolecular exposure to hepatocytes, we asked what role LSEC could play in the pathogenesis of NAFLD. To investigate the early events of NAFLD we used a rat model (Sprague-Dawley) in which animals were maintained on standard and …