Life Sciences Commons^™

In Vitro Antimicrobial Efficacy Of Calcium Hydroxides In Root Dentin, Josef W. Lubisich

Loma Linda University Electronic Theses, Dissertations & Projects

Enterococcus faecalis is the most commonly isolated bacteria in failed root canal treatment. Endodontic intracanal medicaments are commonly tested using standardized bovine cylinders infected with bacteria. The literature is not clear on whether calcium hydroxides are able to remove E. faecalis from the dentinal tubules. One reason for this is that there are several varying forms of calcium hydroxide commonly used. The purpose of the present experiment was to determine whether four commonly used calcium hydroxide products were able to reduce E. faecalis from four incremental samples of dentin and to determine if there was a statistically significant difference between …

Newly Identified Vitamin K-Producing Bacteria Isolated From The Neonatal Faecal Flora, Gordon Cooke, John Behan, Mary Costello

Articles

Fat-soluble vitamin K is an essential component of the blood clotting process. Menaquinones are the naturally occurring form of vitamin K identified in bacteria. Lipid extracts were made from three bacteria originally isolated from the human neonatal gut and identified as Enterobacter agglomerans, Serratia marcescens and Enterococcus faecium. Following preparative thin layer chromatography (TLC), the lipid extracts were subjected to liquid chromatography-mass spectrometry (LC-MS) analysis. Peak analysis of the LC-MS data showed that the three bacteria produce various forms of menaquinone.

Gammaherpesvirus Persistence Alters Key Cd8 T-Cell Memory Characteristics And Enhances Antiviral Protection, Joshua J. Obar, Shinichiro Fuse, Erica K. Leung, Sarah C. Bellfy, Edward J. Usherwood

Dartmouth Scholarship

In herpesvirus infections, the virus persists for life but is contained through T-cell-mediated immune surveillance. How this immune surveillance operates is poorly understood. Recent studies of other persistent infections have indicated that virus persistence is associated with functional deficits in the CD8(+) T-cell response. To test whether this is the case in a herpesvirus infection, we used a mutant murine gammaherpesvirus that is defective in its ability to persist in the host. By comparing the immune response to this virus with a revertant virus that can persist, we were able to dissect the changes in the antiviral CD8(+) T-cell response …

Genome Of Horsepox Virus, Gustavo A. Delhon, C. L. Afonso, Z. Lu, L. Zsak, N. T. Sandybaev, U. M. Kerembekova, V. L. Zaitsev, G. F. Kutish, D. L. Rock

Nebraska Center for Virology: Faculty Publications

Here we present the genomic sequence of horsepox virus (HSPV) isolate MNR-76, an orthopoxvirus (OPV) isolated in 1976 from diseased Mongolian horses. The 212-kbp genome contained 7.5-kbp inverted terminal repeats and lacked extensive terminal tandem repetition. HSPV contained 236 open reading frames (ORFs) with similarity to those in other OPVs, with those in the central 100-kbp region most conserved relative to other OPVs. Phylogenetic analysis of the conserved region indicated that HSPV is closely related to sequenced isolates of vaccinia virus (VACV) and rabbitpox virus, clearly grouping together these VACV-like viruses. Fifty-four HSPV ORFs likely represented fragments of 25 orthologous …

Genome Of Invertebrate Iridescent Virus Type 3 (Mosquito Iridescent Virus), Gustavo A. Delhon, Edan R. Tulman, Claudio L. Afonso, Zhiqiang Lu, James J. Becnel, Bettina A. Moser, Gerald F. Kutish, Daniel L. Rock

Nebraska Center for Virology: Faculty Publications

Iridoviruses (IVs) are classified into five genera: Iridovirus and Chloriridovirus, whose members infect invertebrates, and Ranavirus, Lymphocystivirus, and Megalocytivirus, whose members infect vertebrates. Until now, Chloriridovirus was the only IV genus for which a representative and complete genomic sequence was not available. Here, we report the genome sequence and comparative analysis of a field isolate of Invertebrate iridescent virus type 3 (IIV-3), also known as mosquito iridescent virus, currently the sole member of the genus Chloriridovirus. Approximately 20% of the 190-kbp IIV-3 genome was repetitive DNA, with DNA repeats localized in 15 apparently noncoding regions. Of …

Artificial Burrows And The Deer Mouse: Do Number Of Entrances Influence Use?, Glennis A. Kaufman, Donald W. Kaufman

The Prairie Naturalist

We examined use of artificial burrows by the deer mouse (Peromyscus maniculatus) in mixed grass prairie in north central Kansas. We predicted differential use of burrows, such that the deer mouse would prefer two entrance burrows in non-winter months when predatory snakes were active, but prefer one entrance burrows in winter when cold, windy conditions existed. We installed 20 pairs of artificial burrows (one single entrance and one double entrance) in summer 1988. We examined these burrows on seven dates from autumn 1988 to autumn 1989. All mice (n = 70) used two entrance burrows at least …

Reviews: Natural History Of Javelinas; And Three Dvds On Bats, Louis A. Harveson, Jean Legge

The Prairie Naturalist

Reviews of Javelinas: Collared Peccaries of the Southwest (2006) by Jane Manaster, Texas Tech University Press, Lubbock, Texas, 85 pages (review by Louis A. Harveson) and of The Secret World of Bats (DVD), Building Homes for Bats (DVD), and Kids Discover Bats (DVD) (2005) published by Bat Conservation International (review by Jean Legge).

First Record Of The Least Shrew In Wyoming; And Clostridium Perfingens Type A Enterotoxemia In A Captive Adult White-Tailed Deer, Shauna R. Marquardt, Brian C. Bartels, Cheryl A. Schmidt, Joshua A. Delger, Kevin L. Monteith, Jonathan A. Jenks

The Prairie Naturalist

First Record of the Leaast Shrew in Wyoming by Shauna R. Marquardt, Brian C. Bartels, and Cheryl A. Schmidt; and Clostridium perfingens Type A Enterotoxemia in a Captive Adult White-tailed Deer by Joshua A. Delger , Kevin L. Monteith, and Jonathan A. Jenks.

Correction To Winter Bird Numbers Article

Nebraska Bird Review

Correction to Winter Bird Numbers Article

Editor's Note: We regret that there was an error in the text of the Paul Johnsgard article "Recent Changes in Winter Bird Numbers at Lincoln, Nebraska" published in the March 2006 Nebraska Bird Review (Volume 74 Number 1). The last sentence in the first full paragraph on page 18 should read: "(The House Sparrow is declining annually at a rate of 2.5%, and the House Finch is increasing at an annual rate of 1.15%.)"

Masthead From Nebraska Bird Review September 2006

Nebraska Bird Review

The Nebraska Bird Review is published quarterly by the Nebraska Ornithologists' Union, Inc., as its official journal, and is sent to members not in arrears of dues. Annual subscription rates (on a calendar-year basis only): $15 in the United States, $18 in Canada and $30 in all other countries, payable in advance. Single copies are $4 each, postpaid, in the United States, $5 in Canada, and $8 elsewhere. Send orders for back issues to Mary Lou Pritchard, NOU Librarian, c/o University of Nebraska State Museum, W-436 Nebraska Hall, Lincoln, NE 68588-0514.

Memberships in the NOU (on a calendar-year basis only): …

White-Cheeked Geese In Nebraska, W. Ross Silcock

Nebraska Bird Review

The intent of this paper is to summarize the current state of knowledge regarding "White-cheeked Goose" is used in reference to the various taxa included within the two species Canada Goose (Branta canadensis) and Cackling Goose (B. hutchinsii), as constituted by the American Ornithologist' Union (AOU; 2004). Using culmen measurements, confirmation of both species' and the "expected" subspecies' occurrence in Nebraska was documents, but culmen measurements alone could not confirm the occurrence of any of the "unexpected" subspecies.

TAXONOMY

As many as 10 subspecies of White-cheeked Goose were described by the AOU (1957), five of which ( …

Generic Changes In United States Issini (Hemiptera, Fulgoroidea, Issidae), Vladimir M. Gnezdilov, Lois B. O'Brien

Insecta Mundi

Three new genera of the family Issidae are described for species formerly included in the genus Hysteropterum sensulato: Balduza Gnezdilov and O’Brien, gen. n. (type species: Hysteropterum unum Ball 1910), Stilbometopius Gnezdilov and O’Brien, gen. n. (type species: Issus auroreus Uhler 1876), Abolloptera Gnezdilov and O’Brien, gen. n. (type species: Hysteropterum bistriatum Caldwell 1945),. The genus Tylanira Ball 1936 is redescribed. The male genitalia of Tylanira bifurca Ball 1936 is described and illustrated. Tylana ustulata Uhler 1876 is transfered to Tylanira.Hysteropterum bufo Van Duzee 1923 is transfered to Balduza gen. n. and the male genitalia of the species is …

The Beetle Fauna Of Dominica, Lesser Antilles (Insecta: Coleoptera): Diversity And Distribution, Stewart B. Peck

Insecta Mundi

The beetle fauna of the island of Dominica is summarized. It is presently known to contain 269 genera, and 361 species (in 42 families), of which 347 are named at a species level. Of these, 62 species are endemic to the island. The other naturally occurring species number 262, and another 23 species are of such wide distribution that they have probably been accidentally introduced and distributed, at least in part, by human activities. Undoubtedly, the actual numbers of species on Dominica are many times higher than now reported. This highlights the poor level of knowledge of the beetles of …

Current Status And 25 Year Trends For Soil Acidity, Fertility And Salinity In The Coastal Catchments Of The Peel-Harvey, Robert Summers, David Weaver

All other publications

The current status and trends of soil analyses in the coastal catchment of the Peel Harvey estuary were developed from historical soil data (1982 to 1991) combined with a renewed sampling to greater depth (1 m). This report encompasses the data collected from the first year of the project. Soil phosphorus content was found to be high but has dropped slightly since 1991. Soil pH is very low and is likely to be limiting production but have risen slightly since 1991. Soil potassium content is generally so low that it may be limiting production especially to the west of the …

Co-Variation In Soil Biodiversity And Biogeochemistry In Northern And Southern Victoria Land, Antarctica, Byron J. Adams, J. E. Barrett, Ross A. Virginia, Diana H. Wall, S. C. Cary, A. L. Hacker, J. M. Aislabie

Faculty Publications

Data from six sites in Victoria Land (72-77degrees S) investigating co-variation in soil communities (microbial and invertebrate) with biogeochemical properties show the influence of soil properties on habitat suitability varied among local landscapes as well as across climate gradients. Species richness of metazoan invertebrates (Nematoda, Tardigrada and Rotifera) was similar to previous descriptions in this region, though identification of three cryptic nematode species of Eudorylaimus through DNA analysis contributed to the understanding of controls over habitat preferences for individual species. Denaturing Gradient Gel Electrophoresis profiles revealed unexpectedly high diversity of bacteria. Distribution of distinct bacterial communities was associated with specific …

Detection Of Proteins By Two-Photon Excitation Of Native Fluorescence, Li Li

Theses and Dissertations

Proteins are of primary importance to the structure and function of all living cells. Study of proteins relies on the ability to separate a complex mixture so that individual proteins can be more easily processed by other techniques. Since protein samples often exist at low concentration in a small volume, the trend in chemical analysis is toward micro total analysis systems (µTAS) or lab-on-a-chip devices. Among µTAS separation methods, the relatively new electric field gradient focusing (EFGF) technique has shown potential. It focuses and separates analytes based on their electrophoretic migration in an opposing hydrodynamic flow. The detection principles that …

Structural Inference In Transition Measurement Error Models For Longitudinal Data, Wenqin Pan, Xihong Lin, Donglin Zeng

Harvard University Biostatistics Working Paper Series

No abstract provided.

Estimation In Semiparametric Transition Measurement Error Models For Longitudinal Data, Wenqin Pan, Donglin Zeng, Xihong Lin

Harvard University Biostatistics Working Paper Series

No abstract provided.

Nonparametric Regression Using Local Kernel Estimating Equations For Correlated Failure Time Data, Zhangsheng Yu, Xihong Lin

Harvard University Biostatistics Working Paper Series

No abstract provided.

Causal Inference In Hybrid Intervention Trials Involving Treatment Choice, Qi Long, Rod Little, Xihong Lin

Harvard University Biostatistics Working Paper Series

No abstract provided.

A Comparison Of Methods For Estimating The Causal Effect Of A Treatment In Randomized Clinical Trials Subject To Noncompliance, Rod Little, Qi Long, Xihong Lin

Harvard University Biostatistics Working Paper Series

No abstract provided.

Gene Order Data From A Model Amphibian (Ambystoma): New Perspectives On Vertebrate Genome Structure And Evolution, Jeramiah J. Smith, S. Randal Voss

Biology Faculty Publications

BACKGROUND: Because amphibians arise from a branch of the vertebrate evolutionary tree that is juxtaposed between fishes and amniotes, they provide important comparative perspective for reconstructing character changes that have occurred during vertebrate evolution. Here, we report the first comparative study of vertebrate genome structure that includes a representative amphibian. We used 491 transcribed sequences from a salamander (Ambystoma) genetic map and whole genome assemblies for human, mouse, rat, dog, chicken, zebrafish, and the freshwater pufferfish Tetraodon nigroviridis to compare gene orders and rearrangement rates.

RESULTS: Ambystoma has experienced a rate of genome rearrangement that is substantially lower than mammalian …

Smokejumper Obituary: Harnish Jr., John L. (Cave Junction 1945), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Eye On Estuaries: Nh Tourist, The Bluefish, Soon To Depart Bay, Dave Kellam

PREP Reports & Publications

No abstract provided.

Smokejumper Obituary: Dysart, Charles (Missoula 1953), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Smokejumper Obituary: Crane, Thomas Donald (Fairbanks 1962), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Group Additive Regression Models For Genomic Data Analysis, Yihui Luan, Hongzhe Li

UPenn Biostatistics Working Papers

One important problem in genomic research is to identify genomic features such as gene expression data or DNA single nucleotide polymorphisms (SNPs) that are related to clinical phenotypes. Often these genomic data can be naturally divided into biologically meaningful groups such as genes belonging to the same pathways or SNPs within genes. In this paper, we propose group additive regression models and a group gradient descent boosting procedure for identifying groups of genomic features that are related to clinical phenotypes. Our simulation results show that by dividing the variables into appropriate groups, we can obtain better identification of the group …

Construction Of A Col11a1 Transgene Vector, Cameron Mckell Beck

Theses and Dissertations

Background: Cartilage disorders affect millions of people in the United States alone, with effects ranging from poor skeletal development and joint pain to shortened lifespan and perinatal lethality. Many of these disorders have their root in defects of collagen, type XI collagen being among the most important. A mouse model of such a type XI collagen defect is the chondrodysplasia (cho) mutant. Mice homozygous for this null mutation in the Col11a1 gene do not express the α1 chain of type XI collagen. This results in a functional knockout of type XI collagen, leading to insufficient skeletal development and perinatal lethality. …

Morella Cerifera Small, Gerry Moore

Specimens by Name

No abstract provided.

The Effects Of Wearing A Cooling Vest During The Warm-Up On Long Distance Interval Training, Andrew Robert Tegeder

Theses and Dissertations

Purpose: Reducing body temperature before exercise is called precooling. Past research suggests that reducing body core temperature (Tc) slightly can result in improved running performance. This study evaluated the effects that warming up, while wearing a cooling vest prior to an interval workout, had on Tc, and interval time in long-distance runners. Methods: Nineteen healthy male collegiate cross-country runners were recruited for this study. Each subject warmed up and exercised under two different conditions: (a) an experimental condition in which subjects wore a Nike PreCool® ice vest during warm-up and (b) a control condition. Subjects performed a warm-up followed by …