Life Sciences Commons^™

Working Trees For Water Quality

Working Trees (USDA-NAC)

Water is a precious national resource. Often, human activities degrade the quality of the water in the streams, lakes, estuaries, wetlands, and aquifiers on which we depend. Pollutants from agricultural and urban sources have made many of our waters unsuitable for swimming and fishing. Excessive sedimentation, pesticides, and fertilizers are harming fish and other aquatic life. Changes in land use also have had a dramatic effect on floodwater damage and frequency. Both surface and subsurfaceAgroforestry drinking water supplies are being impacted by human activities.

Water quality is the end result of the individual actions of all the “neighbors” in a …

Smokejumper Obituary: Parker, John E. (Redding 1967), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Kir/Hla Pleiotropism: Protection Against Both Hiv And Opportunistic Infections, Ying Qi, Maureen P. Martin, Xiaojiang Gao, Lisa Jacobson, James J. Goedert, Susan Buchbinder, Gregory D. Kirk, Stephen J. O'Brien, John Trowsdale, Mary Carrington

Biology Faculty Articles

The compound genotype KIR3DS1/HLA-B Bw4-80I, which presumably favors natural killer cell activation, has been implicated in protection against HIV disease. We show that this genotype confers dual protection over the course of HIV disease; early direct containment of HIV viral load, and late specific defense against opportunistic infections, but not AIDS-related malignancies. The double protection of KIR3DS1/Bw4-80I in an etiologically complex disease such as AIDS, along with the disease specificity of its effects is conceptually novel and underscores the intricacy of host immunogenetics against HIV/AIDS.

Perchlorate And Nitrate Remediation Efficiency And Microbial Diversity In A Containerized Wetland Bioreactor, Paula Krauter, Bill Daily Jr., Valerie Dibley, Holly Pinkart, Tina Legler

All Faculty Scholarship for the College of the Sciences

We have developed a method to remove perchlorate (14–27 μg/L) and nitrate (48 mg/L) from contaminated groundwater using a wetland bioreactor. The bioreactor has operated continuously in a remote field location for more than 2 yr with a stable ecosystem of indigenous organisms. This study assesses the bioreactor for long-term perchlorate and nitrate remediation by evaluating influent and effluent groundwater for oxidation-reduction conditions and nitrate and perchlorate concentrations. Total community DNA was extracted and purified from 10-g sediment samples retrieved from vertical coring of the bioreactor during winter. Analysis by denaturing gradient gel electrophoresis of short, 16S rDNA, polymerase-chain-reaction products …

Interview With Ted Morris, Dayton Meat Company, 2006 (Audio), Ted Morris

All Sustainability History Project Oral Histories

Interview of Ted Morris by Matt Witke on August 17th, 2006.

The interview index is available for download.

Phyla Lanceolata (Michx.) Greene, Gordon C. Tucker

Specimens by Name

No abstract provided.

Selenium Metabolism In Trypanosoma: Characterization Of Selenoproteomes And Identification Of A Kinetoplastida-Specific Selenoprotein, Alexey V. Lobanov, Stephan Gromer, Gustavo Salinas, Vadim Gladyshev

Vadim Gladyshev Publications

Proteins containing the 21st amino acid selenocysteine (Sec) are present in the three domains of life. However, within lower eukaryotes, particularly parasitic protists, the dependence on the trace element selenium is variable as many organisms lost the ability to utilize Sec. Herein, we analyzed the genomes of Trypanosoma and Leishmania for the presence of genes coding for Sec-containing proteins. The selenoproteomes of these flagellated protozoa have three selenoproteins, including distant homologs of mammalian SelK and SelT, and a novel multidomain selenoprotein designated SelTryp. In SelK and SelTryp, Sec is near the C-terminus, and in all three selenoproteins, it is within …

Retrotranspositions In Orthologous Regions Of Closely Related Grass Species, Chunguang Du, Zuzana Swigoňová, Joachim Messing

Department of Biology Faculty Scholarship and Creative Works

Background: Retrotransposons are commonly occurring eukaryotic transposable elements (TEs). Among these, long terminal repeat (LTR) retrotransposons are the most abundant TEs and can comprise 50-90% of the genome in higher plants. By comparing the orthologous chromosomal regions of closely related species, the effects of TEs on the evolution of plant genomes can be studied in detail. Results: Here, we compared the composition and organization of TEs within five orthologous chromosomal regions among three grass species: maize, sorghum, and rice. We identified a total of 132 full or fragmented LTR retrotransposons in these regions. As a percentage of the total cumulative …

The Effects Of Prescribed Fire On The Herbaceous Layer In The Southern Appalachian Mountains., Michael Lee Zimmerman

Electronic Theses and Dissertations

Prescribed fire in the southern Appalachians is a frequently used and controversial forest management practice. Research is limited on the effects of prescribed fire in the mesic southern Appalachians, where many of Tennessee's rare and regionally endemic plant species occur. This study examined the effects of prescribed fire on the herbaceous layer. Field work was conducted on six previously burned sites within the Cherokee National Forest in northeast Tennessee. Complimentary non-burned sites were selected based on similarity of physical characteristics and forest structure. The numbers of herbaceous species and individuals and the total numbers of species and individuals were determined …

Dissolved Gallium In The Northwest Pacific And The South And Central Atlantic Oceans: Implications For Aeolian Fe Input And A Reconsideration Of Profiles, Alan M. Shiller, Gautam Raj Bairamadgi

Faculty Publications

[1] The distribution of dissolved gallium, a less-reactive analogue of aluminum, has the potential to reveal information about the averaged dust input to the surface ocean and to complement studies using aluminum as a tracer. New data are presented here on the distribution of dissolved Ga, including six profiles in the south and central Atlantic as well as seven shallow and two deep profiles from the northwest Pacific. The Atlantic data allow for an estimate of Ga in Antarctic Bottom Water ( similar to 25 - 30 pmol kg(-1)) and show reasonably conservative behavior in deep waters. In the northwest …

Seasonal Territoriality In The Carolina Wren, Thryothorus Ludovicianus, To Visual And Vocal Stimuli., Mark Allen Dunaway

Electronic Theses and Dissertations

Carolina Wrens, Thryothorus ludovicianus, are permanent residents throughout their range. They form pair bonds at a young age, maintain these bonds for multiple years, and defend feeding/breeding territories year round. Male Carolina Wrens use songs in territorial defense and have been shown to countersing regularly to both neighbors and intruders. They use various song characteristics to determine the location of another bird and whether its territory has been invaded.

Wrens often approach playbacks silently in apparent searching behavior. In some cases, birds will investigate the playback but fail to countersing. I wanted to determine whether or not wrens would …

Latex Of Sciadopitys Verticillata (Thunb.) Siebold And Zuccarini: Antibiotic Properties, Phytochemistry, And Inhibition Of Adventitious Rooting Of Stem Cuttings., David Ira Yates

Electronic Theses and Dissertations

Sciadopitys verticillata was subjected to three propagation treatments designed to inhibit coagulation of its latex-like sap at the cut ends of the stem cuttings. Twenty-four hour soaking in water prior to rooting hormone application significantly enhanced production of adventitious roots. Old wood stem cuttings from shade-grown trees rooted at higher proportions than stem cuttings collected from sun-grown trees. Height, age, and place of origin of the source trees were not important factors in successful rooting. Antibacterial activity against some human pathogens and soil bacterial species was detected in latex application trials but the antibiotic activity was not related to the …

Determination Of Cholesterol In Foods By Flow Injection Analysis With Perroxyoxalate Chemiluminescence., Abisake Feleke

Electronic Theses and Dissertations

Cholesterol is an important biological molecule with many important functions. However, high serum cholesterol is a health hazard. Thus analysis of cholesterol is important and many analytical techniques have been developed. The objectives of the proposed research are to develop an economical, rapid method for the determination of total cholesterol with good selectivity and enhanced sensitivity. For evaluation of proposed flow injection analysis with peroxyoxalate chemiluminescence (FIA-POCL) method, figures of merit such as accuracy, precision, and linear dynamic range will be assessed. The proposed procedure was then applied to the determination of total cholesterol in foods. The procedure was linear …

Smokejumper Obituary: Schmidt, Carl H. (North Cascades 1948), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Interview With Oscar Lozano, Dayton Meat Company, 2006 (Audio), Oscar Lozano

All Sustainability History Project Oral Histories

Interview of Oscar Lozano by Brad Schuler on August 14th, 2006.

The interview index is available for download.

Characterization Of Alternative Cytosolic Forms And Cellular Targets Of Mouse Mitochondrial Thioredoxin Reductase, Anton A. Turanov, Dan Su, Vadim N. Gladyshev

Vadim Gladyshev Publications

Thioredoxin reductase (TR) and thioredoxin (Trx) define a major cellular redox system that maintains cysteine residues in numerous proteins in the reduced state. Both cytosolic (TR1 and Trx1) and mitochondrial (TR3 and Trx2) enzymes are essential in mammals, but the function of the mitochondrial system is less understood. In this study, we characterized subcellular localization of three TR3 forms that are generated by alternative first exon splicing and that differ in their N-terminal sequences. Only one of these forms resides in mitochondria, whereas the two other isoforms are cytosolic. Consistent with this finding, TR3 did not have catalytic preferences for …

Supplementary Material For: Secis Elements In The Coding Regions Of Selenoprotein Transcripts Are Functional In Higher Eukaryotes, Heiko Mix, Alexey V. Lobanov, Vadim Gladyshev

Vadim Gladyshev Publications

Figure S1. Nucleotide sequence alignment of GPx4 sequences.
For: M. musculus, H. sapiens, G. gallus, A. thaliana, Fowlpox virus, and Canarypox virus

Figure S2. Alignment of GPx4 SECIS elements.
For: H. sapiens, M. musculus, G. gallus, Fowlpox, Canarypox, and MCV

Supplementary Table S1. Viral genome sequences used in a computational analysis
From Abelson murine leukemia virus through Zygosaccharomyces bailii virus Z