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Articles 481 - 510 of 3150
Full-Text Articles in Life Sciences
Cloning Of Nt.Cviqii Nicking Endonuclease And Its Cognate Methyltransferase: M.Cviqii Methylates Ag Sequence, Siu-Hong Chan, Zhenyu Zhu, David Dunigan, James L. Van Etten, Shuang-Yong Xu
Cloning Of Nt.Cviqii Nicking Endonuclease And Its Cognate Methyltransferase: M.Cviqii Methylates Ag Sequence, Siu-Hong Chan, Zhenyu Zhu, David Dunigan, James L. Van Etten, Shuang-Yong Xu
Department of Plant Pathology: Faculty Publications
Chlorella virus NY-2A has a large, highly methylated dsDNA genome (45% of the cytosines are 5-methylcytosine and 37% of the adenines are N6-methyladenine). Here, we report the cloning, expression, and characterization of the NY-2A-encoded CviQII nicking-modification (N-M) system. The nicking endonuclease, Nt.CviQII, recognizes R ↓ AG (R = A or G, ↓ indicating cleavage site) sequences and cleaves the phosphodiester bond 5′ to the adenosine. Because of the difficulty in cloning and expressing the wild-type Nt.CviQII, C-terminal truncation mutants were generated and full-length Nt.CviQII was reconstructed by intein-mediated peptide ligation. The truncation mutants and the reconstructed full-length Nt.CviQII …
Peroxidase-Dependent Apoplastic Oxidative Burst In Arabidopsis Required For Pathogen Resistance, Laurence V. Bindschedler, Julia Dewdney, Kris A. Blee, Julie M. Stone, Tsuneaki Asai, Julia Plotnikov, Carine Denoux, Tezni Hayes, Chris Gerrish, Dewi R. Davies, Frederick M. Ausubel, G. Paul Bolwell
Peroxidase-Dependent Apoplastic Oxidative Burst In Arabidopsis Required For Pathogen Resistance, Laurence V. Bindschedler, Julia Dewdney, Kris A. Blee, Julie M. Stone, Tsuneaki Asai, Julia Plotnikov, Carine Denoux, Tezni Hayes, Chris Gerrish, Dewi R. Davies, Frederick M. Ausubel, G. Paul Bolwell
Department of Biochemistry: Faculty Publications
The oxidative burst is an early response to pathogen attack leading to the production of reactive oxygen species (ROS) including hydrogen peroxide. Two major mechanisms involving either NADPH oxidases or peroxidases that may exist singly or in combination in different plant species have been proposed for the generation of ROS. We identified an Arabidopsis thaliana azide-sensitive but diphenylene iodonium-insensitive apoplastic oxidative burst that generates H2O2 in response to a Fusarium oxysporum cell-wall preparation. Transgenic Arabidopsis plants expressing an anti-sense cDNA encoding a type III peroxidase, French bean peroxidase type 1 (FBP1) exhibited an impaired oxidative burst and …
Inventory And Monitoring Of Aquatic Bird Species On Lakes Mead And Mohave 2004 - 2006, Joseph Barnes
Inventory And Monitoring Of Aquatic Bird Species On Lakes Mead And Mohave 2004 - 2006, Joseph Barnes
Wildlife Monitoring
This project was completed as part of a task agreements awarded by the National Park Service (NPS), Lake Mead National Recreation Area (LAME), to the Public Lands Institute (PLI) at the University of Nevada, Las Vegas (UNLV). This final report was completed under a task agreement ending on September 30, 2006, and was completed in support of an NPS project funded by the Southern Nevada Public Lands Management Act (PLMA) to inventory and monitor shoreline and near-shore natural resources on Lakes Mead and Mohave (DOI 2005). This report details inventory and monitoring efforts on shoreline and aquatic bird species between …
Ecology, Biodiversity, Management, And Restoration Of Aspen In The Sierra Nevada, Wayne D. Shepperd, Paul C. Rogers, David Burton, Dale L. Bartos
Ecology, Biodiversity, Management, And Restoration Of Aspen In The Sierra Nevada, Wayne D. Shepperd, Paul C. Rogers, David Burton, Dale L. Bartos
Aspen Bibliography
This report was commissioned by the USDA Forest Service Lake Tahoe Basin Management Unit to synthesize existing information on the ecology and management of aspen (Populus tremuloides) in the Sierra Nevada of California and surrounding environs. It summarizes available information on aspen throughout North America from published literature, internal government agency reports, and experienced scientists and managers. The historic distribution, abundance, and ecologic role of aspen in the Sierra Nevada are discussed, along with the reproductive physiology of aspen. Issues that affect aspen health and vigor in the Sierra Nevada and elsewhere are covered, along with methodology for …
Music Therapy, Winston J. Craig
Pseudomonas SyringaeHrpj Is A Type Iii Secreted Protein That Is Required For Plant Pathogenesis, Injection Of Effectors,And Secretion Of The Hrpz1 Harpin, Zheng Qing Fu, Ming Guo, James R. Alfano
Pseudomonas SyringaeHrpj Is A Type Iii Secreted Protein That Is Required For Plant Pathogenesis, Injection Of Effectors,And Secretion Of The Hrpz1 Harpin, Zheng Qing Fu, Ming Guo, James R. Alfano
Center for Plant Science Innovation: Faculty Publications
The bacterial plant pathogen Pseudomonas syringae requires a type III protein secretion system (TTSS) to cause disease. The P. syringae TTSS is encoded by the hrp-hrc gene cluster. One of the genes within this cluster, hrpJ, encodes a protein with weak similarity to YopN, a type III secreted protein from the animal pathogenic Yersinia species. Here, we show that HrpJ is secreted in culture and translocated into plant cells by the P. syringae pv. tomato DC3000 TTSS. A DC3000 hrpJ mutant, UNL140, was greatly reduced in its ability to cause disease symptoms and multiply in Arabidopsis thaliana …
Biologic Cycling Of Silica Across A Grassland Bioclimosequence, S. W. Blecker, Rebecca L. Mcculley, O. A. Chadwick, E. F. Kelly
Biologic Cycling Of Silica Across A Grassland Bioclimosequence, S. W. Blecker, Rebecca L. Mcculley, O. A. Chadwick, E. F. Kelly
Plant and Soil Sciences Faculty Publications
The dynamics of biologic Si cycling in grassland ecosystems are largely unknown and likely to impact mineral weathering rates regionally and diatom productivity globally; key regulatory processes in the global Si cycle are closely tied to the global carbon cycle. Across a bioclimatic sequence spanning major grassland ecosystems in the Great Plains, soil biogenic silica depth distributions are similar to that of soil organic carbon; however, unlike soil organic carbon, quantities of soil biogenic silica decrease with increasing precipitation, despite an increase in annual biogenic inputs through litterfall across the same gradient. Though comprising only 1–3% of the total Si …
Utilization Of Sea Scallop (Placopecten Magellanicus Gmelin) Microsatellite Markers For Phylogenetic Applications In Bay Scallops (Argopecten Irradians Lamarck), Lilliana E.J. Kim, James J. Campanella, Paul Ax Bologna
Utilization Of Sea Scallop (Placopecten Magellanicus Gmelin) Microsatellite Markers For Phylogenetic Applications In Bay Scallops (Argopecten Irradians Lamarck), Lilliana E.J. Kim, James J. Campanella, Paul Ax Bologna
Department of Biology Faculty Scholarship and Creative Works
In previous research, microsatellite markers have been used to geographically and phylogenetically characterize and identify populations of sea scallops (Placopecten magellanicus Gmelin). Given that sea scallops are a closely related species to bay scallops (Argopecten irradians Lamarck), we have investigated whether the flanking DNA sequences of sea scallop microsatellite loci have been conserved in the bay scallop. We found that in all seven sets of published sea scallop PCR primers (Pma130, -132, -135, -180, -200, -212, and -275) that were tested with bay scallop DNA, there was sequence conservation and amplification of microsatellite regions. Our initial phylogenetic analyses, …
Optimal Movement Variability: A New Theoretical Perspective For Neurologic Physical Therapy, Nikolaos Stergiou, Regina T. Harbourne, James T. Cavanaugh
Optimal Movement Variability: A New Theoretical Perspective For Neurologic Physical Therapy, Nikolaos Stergiou, Regina T. Harbourne, James T. Cavanaugh
Journal Articles
Variability is a natural and important feature of human movement. Using existing theoretical frameworks as a foundation, we propose a new model to explain movement variability as it relates to motor learning and health. We contend that mature motor skills and healthy states are associated with an optimal amount of movement variability. This variability also has form and is characterized by a chaotic structure. Less than optimal movement variability characterizes biological systems that are overly rigid and unchanging, whereas greater than optimal variability characterizes systems that are noisy and unstable. Both situations characterize systems that are less adaptable to perturbations, …
Combined Control Of Striga Hermonthica And Stemborers By Maize-Desmodium Spp. Intercrops, Zeyaur R. Khan, John A. Pickett, Lester J. Wadhams, Ahmed Hassanali, Charles A.O. Midega
Combined Control Of Striga Hermonthica And Stemborers By Maize-Desmodium Spp. Intercrops, Zeyaur R. Khan, John A. Pickett, Lester J. Wadhams, Ahmed Hassanali, Charles A.O. Midega
All Peer-Reviewed Publications
The African witchweed (Striga spp.) and lepidopteran stemborers are two major biotic constraints to the efficient production of maize in sub-Saharan Africa. Previous studies had shown the value of intercropping maize with Desmodium uncinatum in the control of both pests. The current study was conducted to assess the potential role of other Desmodium spp., adapted to different agro-ecologies, in combined control of both pests in Kenya. Treatments consisted of intercropped plots of a Striga hermonthica- and stemborer-susceptible maize variety and one Desmodium sp. or cowpea, with a maize monocrop plot included as a control. S. hermonthica counts and stemborer damage …
Semantic Interoperability Of Web Services - Challenges And Experiences, Meenakshi Nagarajan, Kunal Verma, Amit P. Sheth, John A. Miller, Jonathan Lathem
Semantic Interoperability Of Web Services - Challenges And Experiences, Meenakshi Nagarajan, Kunal Verma, Amit P. Sheth, John A. Miller, Jonathan Lathem
Kno.e.sis Publications
With the rising popularity of Web services, both academia and industry have invested considerably in Web service description standards, discovery, and composition techniques. The standards based approach utilized by Web services has supported interoperability at the syntax level. However, issues of structural and semantic heterogeneity between messages exchanged by Web services are far more complex and crucial to interoperability. It is for these reasons that we recognize the value that schema/data mappings bring to Web service descriptions. In this paper, we examine challenges to interoperability; classify the types of heterogeneities that can occur between interacting services and present a possible …
Optimal Adaptation In Web Processes With Coordination Constraints, Kunal Verma, Prashant Doshi, Karthik Gomadam, John A. Miller, Amit P. Sheth
Optimal Adaptation In Web Processes With Coordination Constraints, Kunal Verma, Prashant Doshi, Karthik Gomadam, John A. Miller, Amit P. Sheth
Kno.e.sis Publications
We present methods for optimally adapting Web processes to exogenous events while preserving inter-service constraints that necessitate coordination. For example, in a supply chain process, orders placed by a manufacturer may get delayed in arriving. In response to this event, the manufacturer has the choice of either waiting out the delay or changing the supplier. Additionally, there may be compatibility constraints between the different orders, thereby introducing the problem of coordination between them if the manufacturer chooses to change the suppliers. We focus on formulating the decision making models of the managers, who must adapt to external events while satisfying …
Flexible Querying Of Xml Documents, Krishnaprasad Thirunarayan, Trivikram Immaneni
Flexible Querying Of Xml Documents, Krishnaprasad Thirunarayan, Trivikram Immaneni
Kno.e.sis Publications
Text search engines are inadequate for indexing and searching XML documents because they ignore metadata and aggregation structure implicit in the XML documents. On the other hand, the query languages supported by specialized XML search engines are very complex. In this paper, we present a simple yet flexible query language, and develop its semantics to enable intuitively appealing extraction of relevant fragments of information while simultaneously falling back on retrieval through plain text search if necessary. We also present a simple yet robust relevance ranking for heterogeneous document-centric XML.
Characterization Of Mmp37p, A Saccharomyces Cerevisiae Mitochondrial Matrix Protein With A Role In Mitochondrial Protein Import, Michelle R. Gallas, Mary Kathryn Dienhart, Rosemary A. Stuart, Roy M. Long
Characterization Of Mmp37p, A Saccharomyces Cerevisiae Mitochondrial Matrix Protein With A Role In Mitochondrial Protein Import, Michelle R. Gallas, Mary Kathryn Dienhart, Rosemary A. Stuart, Roy M. Long
Biological Sciences Faculty Research and Publications
Many mitochondrial proteins are encoded by nuclear genes and after translation in the cytoplasm are imported via translocases in the outer and inner membranes, the TOM and TIM complexes, respectively. Here, we report the characterization of the mitochondrial protein, Mmp37p (YGR046w) and demonstrate its involvement in the process of protein import into mitochondria. Haploid cells deleted of MMP37 are viable but display a temperature-sensitive growth phenotype and are inviable in the absence of mitochondrial DNA. Mmp37p is located in the mitochondrial matrix where it is peripherally associated with the inner membrane. We show that Mmp37p has a role in the …
Censusing And Measuring Lianas: A Quantitative Comparison Of The Common Methods, Stefan A. Schnitzer, Saara J. Dewalt, Jérôme Chave
Censusing And Measuring Lianas: A Quantitative Comparison Of The Common Methods, Stefan A. Schnitzer, Saara J. Dewalt, Jérôme Chave
Biological Sciences Faculty Research and Publications
Lianas contribute to many aspects of tropical forest diversity and dynamics, and interest in liana ecology has grown substantially in recent years. Methods to census lianas and estimate biomass, however, differ among studies, possibly hindering attempts to compare liana communities. At Nouragues Research Station (French Guiana), we tested the extent to which liana abundance, basal area, and estimated biomass differed depending on stem diameter measurement location, inclusion of ramets, inclusion of lianas rooted within versus passing through the plot, and plot shape. We found that the mean per plot abundance and basal area of lianas were significantly greater when lianas …
Macronuclear Genome Sequence Of The Ciliate Tetrahymena Thermophila, A Model Eukaryote, Jonathan A. Eisen, Robert S. Coyne, Martin Wu, Dongying Wu, Mathangi Thiagarajan, Jennifer R. Wortman, Jonathan H. Badger, Qinghu Ren, Paolo Amedeo, Kristie M. Jones, Luke J. Tallon, Arthur L. Delcher, Steven L. Salzberg, Joana C. Silva, Brian J. Haas, William H. Majoros, Maryam Farzad, Jane M. Carlton, Roger K. Smith, Jyoti Garg, Ronald E. Pearlman, Kathleen M. Karrer, Lei Sun, Gerard Manning, Nels C. Elde, Aaron P. Turkewitz, David J. Asai, David E. Wilkes, Yufeng Wang, Hong Cai, Kathleen Collins, B. Andrew Stewart, Suzanne R. Lee, Katarzyna Wilamowska, Zasha Weinberg, Walter L. Ruzzo, Dorota Wloga, Jacek Gaertig, Joseph Frankel, Che-Chia Tsao, Martin A. Gorovsky, Patrick J. Keeling, Ross F. Waller, Nicola J. Patron, J. Michael Cherry, Nicholas A. Stover, Cynthia J. Krieger, Christina Del Toro, Hilary F. Ryder, Sondra C. Williamson, Rebecca A. Barbeau, Eileen P. Hamilton, Eduardo Orias
Macronuclear Genome Sequence Of The Ciliate Tetrahymena Thermophila, A Model Eukaryote, Jonathan A. Eisen, Robert S. Coyne, Martin Wu, Dongying Wu, Mathangi Thiagarajan, Jennifer R. Wortman, Jonathan H. Badger, Qinghu Ren, Paolo Amedeo, Kristie M. Jones, Luke J. Tallon, Arthur L. Delcher, Steven L. Salzberg, Joana C. Silva, Brian J. Haas, William H. Majoros, Maryam Farzad, Jane M. Carlton, Roger K. Smith, Jyoti Garg, Ronald E. Pearlman, Kathleen M. Karrer, Lei Sun, Gerard Manning, Nels C. Elde, Aaron P. Turkewitz, David J. Asai, David E. Wilkes, Yufeng Wang, Hong Cai, Kathleen Collins, B. Andrew Stewart, Suzanne R. Lee, Katarzyna Wilamowska, Zasha Weinberg, Walter L. Ruzzo, Dorota Wloga, Jacek Gaertig, Joseph Frankel, Che-Chia Tsao, Martin A. Gorovsky, Patrick J. Keeling, Ross F. Waller, Nicola J. Patron, J. Michael Cherry, Nicholas A. Stover, Cynthia J. Krieger, Christina Del Toro, Hilary F. Ryder, Sondra C. Williamson, Rebecca A. Barbeau, Eileen P. Hamilton, Eduardo Orias
Biological Sciences Faculty Research and Publications
The ciliate Tetrahymena thermophila is a model organism for molecular and cellular biology. Like other ciliates, this species has separate germline and soma functions that are embodied by distinct nuclei within a single cell. The germline-like micronucleus (MIC) has its genome held in reserve for sexual reproduction. The soma-like macronucleus (MAC), which possesses a genome processed from that of the MIC, is the center of gene expression and does not directly contribute DNA to sexual progeny. We report here the shotgun sequencing, assembly, and analysis of the MAC genome of T. thermophila, which is approximately 104 Mb in length and …
Level 1an - Diagonal Knit Dishcloth - 101, Debra Proctor
Level 1an - Diagonal Knit Dishcloth - 101, Debra Proctor
All Current Publications
Cotton yarn is a good yarn to use for a dishcloth. It is absorbent, stronger wet than dry, machine washable and can be bleached if needed. These dishcloths are easy and fun to make. Make several and give them as gifts.
Level 1an - Double Yarn Knit Slippers - 102, Debra Proctor, Kristine Saunders
Level 1an - Double Yarn Knit Slippers - 102, Debra Proctor, Kristine Saunders
All Current Publications
This is a simple basic slipper for knitters just learning how to knit. Two strands of worsted weight yarn are used to make the slippers more durable (and warm).
Fitness, Friendship, And Fun: University Sponsored Community Pe Program, Wendi Wilcox Stanley, George M. De Marco, Lloyd L. Laubach, Corinne M. Daprano
Fitness, Friendship, And Fun: University Sponsored Community Pe Program, Wendi Wilcox Stanley, George M. De Marco, Lloyd L. Laubach, Corinne M. Daprano
Health and Sport Science Faculty Publications
This paper describes a University-sponsored community physical education program and the feedback received about it from teachers, children, and the college students who oversaw it. The program, called Fitness, Friendship, and Fun, was staffed by 65 first-year student interns from the University of Dayton; four graduate assistants; and two university professors. It began with 65 male and female elementary students, primarily from the fifth and sixth grades at a nearby elementary school.
Expression Of The Primary Carbohydrate Component Of The Bordetella Bronchiseptica Biofilm Matrix Is Dependent On Growth Phase But Independent Of Bvg Regulation, Yasuhiko Irie, Andrew Preston, Ming H. Yuk
Expression Of The Primary Carbohydrate Component Of The Bordetella Bronchiseptica Biofilm Matrix Is Dependent On Growth Phase But Independent Of Bvg Regulation, Yasuhiko Irie, Andrew Preston, Ming H. Yuk
Biology Faculty Publications
We previously showed that the Bvg virulence control system regulates biofilm formation in Bordetella bronchiseptica (Y. Irie, S. Mattoo, and M. H. Yuk, J. Bacteriol. 186:5692-5698, 2004). Analyses of the extracellular components of B. bronchiseptica biofilm matrix revealed that the major sugar component in the matrix was xylose, and linkage analysis indicated a majority of it to be in a 4-linked polymeric form. The production of xylose was independent of Bvg regulation but instead was dependent on bacterial growth phase. In addition, N-acetyl-glucosamine in the matrix was found to be important for the initial development of the biofilm. These …
Secondary Metabolic Gene Cluster Silencing In Aspergillus Nidulans, J. W. Bok, D. Noordermeer, Shubha Kale Ireland, N. P. Keller
Secondary Metabolic Gene Cluster Silencing In Aspergillus Nidulans, J. W. Bok, D. Noordermeer, Shubha Kale Ireland, N. P. Keller
Faculty and Staff Publications
In contrast to most primary metabolism genes, the genes involved in secondary metabolism and certain nutrient utilization pathways are clustered in fungi. Recently a nuclear protein, LaeA, was found to be required for the transcription of several secondary metabolite gene clusters in Aspergillus nidulans. Here we show that LaeA regulation does not extend to nutrient utilization or the spoC1 sporulation clusters. One of the secondary metabolite clusters regulated by LaeA contains the positive regulatory (i.e. aflR) and biosynthetic genes required for biosynthesis of sterigmatocystin (ST), a carcinogenic toxin. Analysis of ST gene cluster expression indicates LaeA regulation of the cluster …
An ∼140-Kb Deletion Associated With Feline Spinal Muscular Atrophy Implies An Essential Lix1 Function For Motor Neuron Survival, John C. Fyfe, Marilyn Menotti-Raymond, Victor A. David, Lars Brichta, Alejandro A. Schaffer, R. Agarwala, William J. Murphy, William J. Wedemeyer, Brittany L. Gregory, Bethany G. Buzzell, Meghan C. Drummond, Brunhilde Wirth, Stephen J. O'Brien
An ∼140-Kb Deletion Associated With Feline Spinal Muscular Atrophy Implies An Essential Lix1 Function For Motor Neuron Survival, John C. Fyfe, Marilyn Menotti-Raymond, Victor A. David, Lars Brichta, Alejandro A. Schaffer, R. Agarwala, William J. Murphy, William J. Wedemeyer, Brittany L. Gregory, Bethany G. Buzzell, Meghan C. Drummond, Brunhilde Wirth, Stephen J. O'Brien
Biology Faculty Articles
The leading genetic cause of infant mortality is spinal muscular atrophy (SMA), a clinically and genetically heterogeneous group of disorders. Previously we described a domestic cat model of autosomal recessive, juvenile-onset SMA similar to human SMA type III. Here we report results of a whole-genome scan for linkage in the feline SMA pedigree using recently developed species-specific and comparative mapping resources. We identified a novel SMA gene candidate, LIX1, in an ~140-kb deletion on feline chromosome A1q in a region of conserved synteny to human chromosome 5q15. Though LIX1 function is unknown, the predicted secondary structure is compatible with …
Cd80 And Cd86 Control Antiviral Cd8+ T-Cell Function And Immune Surveillance Of Murine Gammaherpesvirus 68, Shinichiro Fuse, Joshua J. Obar, Sarah Bellfy, Erica K. Leung, Weijun Zhang, Edward J. Usherwood
Cd80 And Cd86 Control Antiviral Cd8+ T-Cell Function And Immune Surveillance Of Murine Gammaherpesvirus 68, Shinichiro Fuse, Joshua J. Obar, Sarah Bellfy, Erica K. Leung, Weijun Zhang, Edward J. Usherwood
Dartmouth Scholarship
The interactions between CD80 and CD86 on antigen-presenting cells and CD28 on T cells serve as an important costimulatory signal in the activation of T cells. Although the simplistic two-signal hypothesis has been challenged in recent years by the identification of different costimulators, this classical pathway has been shown to significantly impact antiviral humoral and cellular immune responses. How the CD80/CD86-CD28 pathway affects the control of chronic or latent infections has been less well characterized. In this study, we investigated its role in antiviral immune responses against murine gammaherpesvirus 68 (MHV-68) and immune surveillance using CD80/CD86−/− mice. In the …
Influence Of Optical Cues From Conspecific Females On Escalation Decisions During Male-Male Interactions Of Jumping Spiders, Fiona R. Cross, Robert R. Jackson, Simon D. Pollard, Mark W. Walker
Influence Of Optical Cues From Conspecific Females On Escalation Decisions During Male-Male Interactions Of Jumping Spiders, Fiona R. Cross, Robert R. Jackson, Simon D. Pollard, Mark W. Walker
All Peer-Reviewed Publications
An important prediction from game theory is that the value of a resource influences the level to which male-male conflict escalates. An earlier experimental study showed that males of Hypoblemum albovittatum, a common salticid in New Zealand, can discern by sight alone whether a female is in the vicinity and that, having detected a female's presence, males escalate (i.e., adopt behaviour that is likely to put them at greater risk of injury). Seeing a bare moving cork, a conspecific male or a prey item also elicits escalation, but not to the level elicited by seeing a conspecific female. Here the …
Distribution, Morphology, And Genetic Affinities Of Dwarf Embedded Fucus Populations From The Northwest Atlantic Ocean, Arthur C. Mathieson, Clinton J. Dawes, Aaron L. Wallace, Anita S. Klein
Distribution, Morphology, And Genetic Affinities Of Dwarf Embedded Fucus Populations From The Northwest Atlantic Ocean, Arthur C. Mathieson, Clinton J. Dawes, Aaron L. Wallace, Anita S. Klein
New Hampshire Agricultural Experiment Station Publications
Dwarf embedded Fucus populations in the Northwest Atlantic Ocean are restricted to the upper intertidal zone in sandy salt marsh environments; they lack holdfasts and are from attached parental populations of F. spiralis or F. spiralis x F. vesiculosus hybrids after breakage and entanglement with halophytic marsh grasses. Dwarf forms are dichotomously branched, flat, and have a mean overall length and width of 20.3 and 1.3 mm, respectively. Thus, they are longer than Irish (mean 9.3 mm) and Alaskan (mean 15.0 mm) populations identified as F cottonii. Reciprocal transplants of different Fucus taxa in a Maine salt marsh confirm that …
Inducible Yeast System For Viral Rna Recombination Reveals Requirement For An Rna Replication Signal On Both Parental Rnas, Hernan Garcia-Ruiz, Paul Ahlquist
Inducible Yeast System For Viral Rna Recombination Reveals Requirement For An Rna Replication Signal On Both Parental Rnas, Hernan Garcia-Ruiz, Paul Ahlquist
Department of Plant Pathology: Faculty Publications
To facilitate RNA recombination studies, we tested whether Saccharomyces cerevisiae, which supports brome mosaic virus (BMV) replication, also supports BMV RNA recombination. Yeast strains expressing BMV RNA replication proteins 1a and 2apol were engineered to transiently coexpress two independently inducible, overlapping, nonreplicating derivatives of BMV genomic RNA3. B33' lacked the coat protein gene and negative-strand RNA promoter. B35' lacked the positive-strand RNA promoter and had the coat gene replaced by the selectable URA3 gene. After 12 to 72 h of induction, B33' and B35' transcription was repressed and Ura+ yeast cells were selected. All Ura+ cells …
Synergism Of Gingipains In Endothelial Cell Adhesion Disruption And Apoptosis, Shaun Michellee Sheets
Synergism Of Gingipains In Endothelial Cell Adhesion Disruption And Apoptosis, Shaun Michellee Sheets
Loma Linda University Electronic Theses, Dissertations & Projects
The presence of Porphyromonas gingivalisin the periodontal pocket and the high levels of gingipain activity detected in gingival crevicular fluid could implicate a role for gingipains in the destruction of the highly vascular periodontal tissue. To explore the effects of these proteases on endothelial cells, we exposed bovine coronary artery endothelial cells (BCAEC) and human microvascular endothelial cells (HMVEC) to gingipain-active extracellular protein preparations and/or purified gingipains from P. gingivalis. Treated cells exhibited a rapid loss of cell adhesion properties that was followed by apoptotic cell death. Cleavage of N- and VE-cadherin and integrin (31 was observed in immunoblots …
Assessment Of Resistance In Multi Drug Resistant Tuberculosis Patients, Seema Irfan, Qaiser Hassan, Rumina Hasan
Assessment Of Resistance In Multi Drug Resistant Tuberculosis Patients, Seema Irfan, Qaiser Hassan, Rumina Hasan
Department of Pathology and Laboratory Medicine
Objective: To study MDR-TB isolates and to identify primary and secondary resistance at microbiology laboratory Aga Khan University, Karachi, Pakistan.
Methods: All samples positive for Mycobacterium tuberculosis (MTB) received during January - September 2004 were reviewed for drug resistance pattern as well as for history of previous antituberculous drugs exposure.
Results: Out of 216 Mycobacterium tuberculosis cultures, 138 (64%) showed resistance to one or more agents. Multi drug resistance (MDR) was observed in 102 (47%) isolates. Of 138 drug resistant isolates; primary resistance to any one or more agent was noted in 31(39%) and secondary (acquired) resistance in 107 …
Microbial Growth And Biofilm Formation In Geologic Media Is Detected With Complex Conductivity Measurements, Caroline A. Davis, Estella A. Atekwana, Eliot A. Atekwana, Lee D. Slater, Silvia Rossbach, Melanie R. Mormile
Microbial Growth And Biofilm Formation In Geologic Media Is Detected With Complex Conductivity Measurements, Caroline A. Davis, Estella A. Atekwana, Eliot A. Atekwana, Lee D. Slater, Silvia Rossbach, Melanie R. Mormile
Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works
Complex conductivity measurements (0.1-1000 Hz) were obtained from biostimulated sand-packed columns to investigate the effect of microbial growth and biofilm formation on the electrical properties of porous media. Microbial growth was verified by direct microbial counts, pH measurements, and environmental scanning electron microscope imaging. Peaks in imaginary (interfacial) conductivity in the biostimulated columns were coincident with peaks in the microbial cell concentrations extracted from sands. However, the real conductivity component showed no discernible relationship to microbial cell concentration. We suggest that the observed dynamic changes in the imaginary conductivity (σ″) arise from the growth and attachment of microbial cells and …
Statistical Approach To Background Subtraction For Production Of High-Quality Silhouettes For Human Gait Recognition, Jennifer J. Samler
Statistical Approach To Background Subtraction For Production Of High-Quality Silhouettes For Human Gait Recognition, Jennifer J. Samler
Theses and Dissertations
This thesis uses a background subtraction to produce high-quality silhouettes for use in human identification by human gait recognition, an identification method which does not require contact with an individual and which can be done from a distance. A statistical method which reduces the noise level is employed resulting in cleaner silhouettes which facilitate identification. The thesis starts with gathering video data of individuals walking normally across a background scene. From there the video is converted into a sequence of images that are stored as joint photographic experts group (jpeg) files. The background is subtracted from each image using a …