Life Sciences Commons^™

Non-Additive Toxicity Of Bi-Metal Mixtures To Fathead Minnows, Natalie Rae Lynch

Master's Theses

Much research has been conducted to assess the toxicity of metals to aquatic organisms. Most of the research has focused on the toxicity of individual metals. Recently, attention has been paid to metal mixture toxicity because metals are usually present as mixtures in contaminated environments. The literature review indicates that metal mixtures may be additive, synergistic, or antagonistic to freshwater species. However, the data is not consistent and is dependent on the metal and organisms. The goal of this research is to use a systematic experimental design to characterize the toxicity of Cu, Zn, Cd and Ni mixtures to Pimephales …

Identification Of Clinical Markers That Predict The Outcomes Of Methicillin-Resistant Staphylococcus Aureus Infections And Identification Of Synergistic Antibiotic Combinations For The Treatment Of These Infections, Virginia Long

Master's Theses

Methicillin-resistant Staphylococcus aureus(MRSA) is a prominent nosocomial infection. Vancomycin is the mainstay of treatment, but it has a high rate of failure. Combination therapies can be used, but synergy is often not tested in clinical laboratories. Physicians are at a disadvantage because they cannot predict MRSA outcomes. The first aim of the study was to identify clinical and demographic markers that could predict infection recurrence or prolonged bacteremia. This was accomplished using retrospective chart review and statistical analysis. The second aim of the study was to identify which combinations, of those commonly used for MRSA treatment at Loyola, demonstrate the …

Elucidating The Transcriptional Regulation Of Col2a1a In Zebrafish, Sonja Dabizljevic

Master's Theses

Cartilage is an important tissue in vertebrates beginning in the early embryo where it lays down the scaffolding for the skeleton, and continuing through adulthood where it makes up joints and intervertebral disks. The major component of cartilage is a filamentous protein known as Collagen type II, alpha 1 (Col2a1). Mutations in col2a1 in humans can lead to multiple congenital disorders and the early onset of joint and retinal deterioration. Our laboratory is interested in understanding the conserved transcriptional regulation of this critical structural gene utilizing the zebrafish, Danio rerio. Previously, we identified a 310 bp regulatory region (R2) 1.7 …

Life Histories, Diets, And Secondary Production Of Odonata Along A Temperature Gradient On The Copper River Delta, Alaska, Nicole Emilie Furlan

Master's Theses

Dragonflies (Odonata: Epiprocta) and damselflies (Odonata: Zygoptera) are a conspicuous aspect of the biota in ponds on southcentral Alaska's Copper River Delta (CRD). Odonate densities, secondary production, and diets were assessed in sixteen ponds classified by delta region (east vs. west) and landscape type (outwash plain (OP) vs uplifted marsh (UM)).

Enallagma boreale (Coenagrionidae) comprised 48.5% of collected odonates. Leucorrhinia hudsonica (Libellulidae) and Aeshna juncea (Aeshnidae) comprised 36.6% and 10.4% of collected odonates, respectively. L. hudsonica densities and secondary production were significantly higher (p<0.001) in west UM ponds than in other pond types. Ostracods (Ostracoda) and water boatmen (Corixidae) dominated west OP A. juncea diets. Midge larvae (Chironomidae) dominated A. juncea diets in remaining pond types, occurring in 68% of foreguts. 27% of A. juncea foreguts demonstrated intraguild predation, and 6% of foreguts demonstrated cannibalism. Foreguts containing threespine stickleback (Gasterosteus aculeatus) revealed A. juncea's apex predator role in CRD ponds.

Reconsidering The Model Of Trim5Α Assembly: The Role Of The Linker2 (L2) Region In Trim5Α Assembly, Laura Johnsen

Master's Theses

The TRIM5α protein from rhesus macaques (TRIM5αrh) exhibits a remarkable ability to potently inhibit infection by Human Immunodeficiency Virus Type-1 (HIV-1). Extensive studies have shown that TRIM5α is capable of self-associating at many levels, eventually leading to the formation of a hexameric assembly that can superimpose on the hexameric lattice of the HIV-1 capsid. The mechanism underlying the self-association of TRIM5α and the molecular determinants of self-association remain to be completely understood. In this study, we show that the Linker 2 (L2) region of TRIM5rh is important for dimerization and higher order self-association, both of which are independent processes. Additionally, …

Characterizing The Mechanisms By Which Community Associated Methicillin-Resistant Staphylococcus Aureus Influences Keratinocyte Innate Immune Responses During Recurrent Infection, Ashley Lynn Larm

Master's Theses

Community associated–methicillin resistant Staphylococcus aureus (CA–MRSA) infection has become a major health concern. In human epidermal keratinocytes, S. aureus is mainly recognized through toll–like receptor 2 (TLR2) and its co–receptor, CD14. We hypothesize that CA–MRSA isolates cause recurrent infections by interrupting TLR2–mediated inflammation in keratinocytes. Recurrent CA–MRSA bacterial culture supernatant exposure to keratinocytes in vitro resulted in significant decreases in pro and anti–inflammatory cytokine and HMGB1 secretion from keratinocytes as assessed by ELISAs. Recurrent CA–MRSA live infection did not result in significant changes in cytokine or HMGB1 secretion, surface receptor expression, or NFκB activation post infection as assessed by ELISA …

History Of Freshwater Invasions In Illinois: Learning From The Past To Inform The Future, Abigail Jacobs

Master's Theses

Rates of introduction and spread of non-native species continue to increase worldwide, with freshwater ecosystems highly impacted. Many non-native species crossed between the Great Lakes and Mississippi watersheds by traveling through waterways in Illinois. I assembled a comprehensive database of occurrences of aquatic non-native species (n=99) in Illinois inland waters. The arrival of non-native species accelerated since 1873. The Great Lakes Basin was the main source of established species into Illinois. From 1990-2012 the dominant vector was unintentional release of established species non-native to North America. Sixty of the 99 introduced species established. Eighteen established species had high or very …

Denitrification In Urban Coastal Environments: A Functional Gene Study, Samantha Denise Lindemann

Master's Theses

Eutrophication is a major anthropogenic stressor on aquatic ecosystems worldwide. Jamaica Bay is located in the southeastern portion of New York City, NY (NYC). Like many other estuaries, Jamaica Bay is impacted by anthropogenic N inputs from various sources. Despite eutrophic conditions, improvements in water quality over the last few decades have prompted government agencies to promote oyster restoration to help reduce anthropogenic N in NYC waters. The effect of eastern oysters on sediment bacterial communities responsible for denitrification in Jamaica Bay was previously unknown. Those samples exposed to oysters were predicted to have high denitrification gene abundances, and thus …

Characterization Of The Female Urinary Microbiota And Their Association With The Female Bladder Uroepithelium, Evann Elizabeth Hilt

Master's Theses

The current clinical dogma assumes that urine is sterile in the absence of clinically relevant infection. However, recent evidence has demonstrated the existence of a female urinary microbiota in women with and without lower urinary tract symptoms. With the knowledge that the lower urinary tract possesses its own unique microbiota, I hypothesize that certain bacterial species of the female microbiota may be the cause or play a role in lower urinary tract syndromes such as overactive bladder syndrome (OAB). About 40-50% of OAB patients do not respond to conventional anti-muscarinic and beta-3 adrenergic agonist drug treatment. One possible explanation for …

An Investigation Of The Role Of Vibrio Vulnificus Rbda And Vibrio Parahaemolyticus Sypa In Biofilm Formation, Cecilia Thompson

Master's Theses

Vibrio vulnificus and Vibrio parahaemolyticus are both bacteria that cause human infection. V. vulnificus has a polysaccharide locus, rbd, responsible for bacterial aggregation, a form of biofilm. This locus is conserved in V. parahaemolyticus and in the non-pathogen V. fischeri.

In V. fischeri, the polysaccharide locus, syp, has been extensively characterized and shown to be important for biofilm formation. In V. fischeri, the first gene, sypA, is critical for biofilm formation. V. fischeri biofilm-proficient strains form wrinkled colonies. In contrast, sypA mutants form smooth colonies, indicating a lack of biofilm formation.

To understand the function of RbdA and SypAVP, proteins …

Land Grabs And Implications On Food Sovereignty And Social Justice In Senegal, Joanna Lafrancesca

Master's Theses

This thesis focuses on the case study of Senegal to examine the implications of large- scale land acquisitions on the livelihoods of small-scale farmers. I investigate the diverse perspectives of market enthusiasts, human rights organizations, peasants, the state, and international financial organizations on large-scale land acquisitions. Based on primary research, I argue that the state of Senegal plays an active role in permitting “land grabs” and that they pose a threat to food sovereignty among Senegalese host communities. Lastly, I argue there needs to be a broader understanding of long-term consequences and risks to insure social justice in areas affected …

Biochemical Assay Optimization And Computational Screening Efforts To Identify Potential Luxs Inhibitors, Keeshia Q. Wang

Master's Theses

Quorum sensing (QS) is a process of coordination of bacterial gene expression in response to cell population. System two QS is regulated by the small signaling molecule autoinducer-2 (AI-2) and is implicated in the infectious behaviors of various bacterial species. AI-2 is biosynthesized from S-ribosylhomocysteine (SRH) by the enzyme LuxS and induces interspecies cell-to-cell communication. Inhibition of LuxS would therefore inhibit interspecies QS. Herein, a search for novel molecular species that will competitively bind with SRH in the LuxS binding site is performed in silico. Computational screening results are then validated in vitro using an optimized LuxS inhibition …

An Assessment Of Habitat Suitability For Pronghorn Populations Of The Central Valley Region Of California, Virginia Burroughs

Master's Theses

Efforts to reintroduce and maintain populations of pronghorn (Antilocapra americana) to the California Central Valley, specifically the Carrizo Plain National Monument (CPNM) and the Mojave Desert (Antelope Valley) portion of Tejon Ranch, have largely been unsuccessful due to dwindling numbers of translocated animals. The objective of this study was to improve upon previous models for the CPNM using aerial survey data and then apply the model to the Tejon Ranch. Aerial survey data collected from 2000-2010 on the CPNM was used to establish “use” and “non-use” areas in the model. Model variables included vegetation type (forest, shrub, grassland, …

The Behavioral And Physiological Effects Of Long-Distance Translocation On Western Rattlesnakes (Crotalus Oreganus), Kory Hayden Heiken

Master's Theses

Long-distance translocation (LDT), the relocation of an animal outside of its home range, is a popular strategy for mitigating conflict between humans and venomous snakes. While LDT has been demonstrated to prevent a snake’s return to the location of capture, it may result in increased mortality, magnitude and frequency of movements, and activity range sizes. Thus, it has generally been discouraged. However, the effects of LDT on stress physiology and thermoregulation have gone largely unstudied in reptiles. To elucidate these effects, we conducted an experimental LDT on Western Rattlesnakes (Crotalus oreganus) on Vandenberg Air Force base in California. …

Variability Of Practice And Strength Training Periodization: When Theories Collide, Katherine Lauren Streder

Master's Theses

The primary purpose of this study was to examine whether variability of practice enhances neuromuscular adaptations when compared to traditional strength training. The secondary purpose was to investigate whether there was a difference in perception of exertion between the two strength programs. Forty one subjects (23 women and 18 men) were assigned to either the control group or one of two treatment groups by a blocked-random method. Subject's one repetition maximum (1RM) for the kettlebell press and leg press were measured at baseline, after 4 weeks of training, and after 8 weeks of training. The treatment groups completed 8 weeks …

Age, Growth, Mortality, And The Effects Of Trawling On Mortality Of The Longspine Porgy, Stenotomus Caprinus, Cher Marie Newman

Master's Theses

Stenotomus caprinus (Longspine Porgy) is one of the most abundant bycatch species caught in trawl fisheries in the northern Gulf of Mexico (GOM). Biological data of length, weight, and abundance have been collected since 1972 on the Southeast Area Monitoring and Assessment Program (SEAMAP) surveys with a variety of GOM species, including Longspine Porgy, using trawl nets during groundfish surveys. In 2010-2011 five Longspine Porgy were obtained from Groundfish and Pelagic surveys at each station for further analysis. Age distribution was determined through von Bertalanffy length and weight-age models. The samples were separated into two regions of the GOM to …

Assessing Respiration Rates And Nutrient Dynamics Of Aritifical Reef Biofilms And Bacterioplankton In The Mississippi Sound, Lynn Elizabeth Wilking

Master's Theses

Artificial reefs are primarily used to provide a suitable habitat for target fish populations, but the structures can also improve water quality and benefit non-target organisms. Laboratory incubation experiments were conducted in the presence of biofilm on rubble and in its absence to examine bacterial growth, community respiration, and nutrient dynamics at four artificial reef habitats in the Mississippi Sound. Biofilm samples were also collected from settlement plates deployed at each site and were analyzed for 813C and 81sN stable isotope content. Respiration rates were always higher in the presence of biofilm but bacterial abundance often declined over time, and …

The Effects Of 8 Weeks Of Low Dose Supplementation Of Creatine And Sodium Bicarbonate On Exercise Performance, Amanda Jessica Morris

Master's Theses

Short-term (3-7 days), high doses of creatine (20g/d) and/or sodium bicarbonate (0.5g/kg body weight) supplementation increase exercise performance during short term high intensity activities; however, it remains unclear whether long-term, low doses of these supplements have a positive impact on exercise performance. The purpose of this study was to determine the effects of long-term (8 weeks), low dose creatine supplementation on exercise performance, and whether combining creatine and sodium bicarbonate supplementation has an additive effect. Sixty-three healthy, habitually active, adults (28 M, 35 W; 22+2 years; 23+ 3 BMI) were randomly assigned by sex to one of three supplement groups: …

Effect Of Antimicrobials And Sodium Replacement Agents On The Survival Of Pathogenic Bacteria In Low Sodium Low-Moisture Part-Skim (Lmps) Mozzarella Cheese, Tiffany M J Taylor

Master's Theses

Recent increases in chronic cardiovascular diseases, such as hypertension, have put pressure on the food industry to reduce sodium levels. Dairy products, though full of vital nutrients, are perceived as being high in sodium. However, the reduction of salt in dairy products could potentially alter the microbial stability, as well as cause unfavorable changes in flavor. In order to reduce the sodium level, while maintaining acceptable flavor and microbial stability, salt replacers and alternative antimicrobial agents may need to be introduced into the food matrix. To identify potential antimicrobials for use in reduced sodium dairy products, this study evaluated the …

Lipid Production By Microalgae Treating Municipal Wastewater, James Edward Kelley

Master's Theses

Microalgae hold much promise as a feedstock in liquid biofuel production. Lipid content of microalgae cells range from 30-80% dry weight of biomass. It is projected that microalgae can produce between 1,000-6,500 gallons/acre/year of oil. Currently, production of industrial algae operates in open raceway ponds that use minimal capital and energy inputs to culture algae. Raceway ponds can also be used to grow microalgae from municipal waste streams. Although high biomass productivity can be achieved in these systems, there remains a large production gap between large volumes of biomass cultivation and high lipid content from microalgae cells. Low lipid content …

Protective Effects Of Sphingomyelin Against Uv Photodamage In Human Keratinocytes, Kathleen De Guzman

Master's Theses

Ultraviolet (UV) radiation has been demonstrated in numerous studies to be a major risk factor for non-melanoma skin cancer development. Despite the emergence of current UV-preventative strategies, such as sunscreens and skin-protective clothing, the incidence of non-melanoma skin cancer has continued to rise. This has encouraged investigations on alternative methods for UV prevention. In particular, bovine milk sphingomyelin has been studied for its potential in protecting human skin against UV photodamage. While the previous studies have suggested that sphingomyelin exhibits UV-protective properties in a human skin equivalent model, the exact mechanisms behind sphingomyelin’s photoprotective effects are yet unknown.

This thesis …

The Influence Of Hydrogeomorphology, Soil Redox Conditions, And Salinity On The Spatial Zoning Of Saltgrass, Salt Rush, And Cattails In Scotts Creek Marsh, Swanton Pacific Ranch, Ca, Mark D. Gormley

Master's Theses

Scotts Creek Marsh (SCM) is a small coastal wetland ecosystem in Davenport, CA. The vegetation of SCM is dominated by three halophytic zones comprised of saltgrass, salt rush, cattails. The objectives of the study were (i) to investigate the variables that influence the zoning of the three dominant halophyte communities in SCM and (ii) to the test the effectiveness of Indicator of Reduction in Soil (IRIS) tubes to indicate the reduction of S. The study examined the following parameters from April 6 to July 21, 2013: (i) the HGM of Scotts Creek Marsh, (ii) soil oxidation and reduction (redox) conditions, …

Conjugated Linoleic Acids Alter Body Composition Differently According To Physiological Age In Moulard Ducks, Jeff Fesler, Daniel Peterson

Master's Theses

Conjugated linoleic acids (CLA) have been shown to have remarkable yet inconsistent metabolic effects in mice, rats, hamsters, chickens, cattle, and humans. In particular, effects on lipogenesis vary with tissue, physiological state and specie. In this study we tested the hypothesis that CLA would differentially affect ducks of the same genetic background but of differing age. Growing (7 wk) and maintenance (11 wk) Moulard ducks were grouped by age and fed a standard diet supplemented with either 5% soybean oil (control) or 5% CLA isomer mixture. Animals were harvested after 3 weeks or 6 weeks for assessment of body composition …

Determining Parentage And The Effects Of Relatedness On Play Partner Preference In Belding's Ground Squirrels, Jessica Weidenbach

Master's Theses

I investigated 1) multiple paternity in Belding’s ground squirrels (Urocitellus beldingi) and 2) social partner preferences in juvenile U. beldingi. Prior work with blood allozymes demonstrated multiple paternity in U. beldingi litters. I evaluated paternity using microsatellite DNA analysis, which is more accurate than examining blood allozymes. My results indicate that multiple paternity in U. beldingi is more extensive than previously shown, occurring in about 90% of litters with more than one juvenile, and averaging 2.95 fathers in multiply sired litters. I also evaluated the hypothesis that play and other social behavior promotes bonding among juvenile female …

Identifying Chromosome Rearrangements In The Allopolyploid Brassica Napus Using Pyrosequencing, Alexandra R. Barbella

Master's Theses

Allopolyploids form through the hybridization of two or more diploid genomes. A challenge to reproduction in allopolyploids is that pairing can occur between homologous chromosomes or homeologous chromosomes (i.e.different subgenomes.). Crossover between homeologous chromosomes can result in chromosome rearrangements that lower fertility and overall fitness. Rearrangements can alter the dosage of either entire chromosomes or just parts of chromosomes. Understanding the frequency and extent of rearrangements will help to explain the evolution and genome stabilization of agriculturally important allopolyploid species. Pyrosequencing is a useful tool in the study dosage changes in allopolyploids because it allows quantification of the relative contribution …

Effect Of Warm-Up Activity On Vertical Ground Reaction Forces In Basketball Players During Drop Jump Landings, Jacob Wesley Hinkel-Lipsker

Master's Theses

ABSTRACT

Effect of Warm-Up Activity on Vertical Ground Reaction Forces in Basketball Players During Drop Jump Landings

Jacob Hinkel-Lipsker

Purpose: The purpose of this study was to examine the effect of certain warm-up activities on vertical ground reaction forces (GRF_v) during a drop jump landing. Methods: Eight women and twelve men (mean age 22.6 ± 1.82 years) completed three warm-up activities on three separate days in a counterbalanced order: a passive stretching warm-up, a dynamic warm-up, and a no warm-up control. After completing each activity, participants were asked to perform eight drop jump landings on a force platform. …

Hcmv Promotes Breast Cancer Metastasis: Impacts Of Cmvil-10 In The Tumor Microenvironment, Robin Bishop

Master's Theses

Human cytomegalovirus (HCMV) is a highly species-specific, common human pathogen. While a large majority of people are infected with HCMV worldwide, infection is typically asymptomatic in immune competent individuals. To enhance virus persistence and avoid immune detection by the host, HCMV exploits the strategy of encoding homologs of cellular cytokines. One factor produced by HCMV-infected cells is the viral cytokine cmvIL-10, which acts through the cellular IL-10 receptor to induce downstream cell signaling. In instances where HCMV-infected cells infiltrate the tumor microenvironment, the presence of cmvIL-10 in may initiate events that promote a more invasive tumor phenotype. Cancer cells that …

The Effects Of Microfluidization And Homogenization On The Composition And Structure Of Liposomal Aggregates From Whey Buttermilk And Commercial Buttermilk, Tracey Mai T. Nguyen

Master's Theses

Milk derived ingredients from the production of cheese and butter can be used as vehicles for nutrients. Buttermilk is a nutritious product of milk that comes from the churning of cream into butter. One of the advantages of buttermilk is that it is enriched in milk fat globule components, such as phospholipids and forms emulsions with fat when treated with high shear. The objective of this work was to explore the effects of shear on regular buttermilk and whey buttermilk in terms of liposomal aggregate size and chemical composition. The effects of microfluidization at 2000 psi and homogenization at 2000 …

An Insight Into The Microbial Diversity And Expression Of Cysteine Protease Inhibitors (Cystatin) In Rickettsia Parkeri Infected Amblyomma Maculatum, Khemraj Budachetri

Master's Theses

Amblyomma maculatum (Gulf Coast tick) is an emerging tick species of public health significance in United States. It is a competent vector of Rickettsia parkeri, an etiological agent of a human rickettsiosis. In this study, we investigated the spotted fever group of rickettsial diversity in A. maculatum based on rickettsial ompA gene PCR. Our results showed A. maculatum harbors R. parkeri, R. amblyommii, and R. endosymbiont of A. maculatum. While only R. parkeri was detected in female salivary glands which suggest its ability to traffic from midgut to salivary glands via hemocoel. The presence of R. parkeri was further …

Characterizing The Persistence Of Sewage Enterococci In Mississippi Coastal Waters, Kimberly Anne-Marie Lewis

Master's Theses

Enterococci are microbiological indicators of marine recreation water quality.
Their reliability as fecal indicators is questioned as they are shown to persist in the environment. Multiple laboratory studies on their persistence have been done but few under natural environmental conditions. The purpose of this study was to investigate how long sewage enterococci and enterococcal DNA persist in beach water and to determine whether there is a difference in the genetic diversity and hardiness of sewage vs. environmental isolates. To study persistence, sewage was diluted with beach water, placed in microcosms, and deployed at a beach site in Longbeach and Pass …